ATE407199T1 - Pullulanase variante mit vorbestimmten eigenschaften und verfahren zur deren herstellung - Google Patents

Pullulanase variante mit vorbestimmten eigenschaften und verfahren zur deren herstellung

Info

Publication number
ATE407199T1
ATE407199T1 AT01901118T AT01901118T ATE407199T1 AT E407199 T1 ATE407199 T1 AT E407199T1 AT 01901118 T AT01901118 T AT 01901118T AT 01901118 T AT01901118 T AT 01901118T AT E407199 T1 ATE407199 T1 AT E407199T1
Authority
AT
Austria
Prior art keywords
production
predetermined properties
pullulanase variant
pullulanase
variant
Prior art date
Application number
AT01901118T
Other languages
English (en)
Inventor
Allan Svendsen
Carsten Andersen
Borchert Torben Vedel
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE407199T1 publication Critical patent/ATE407199T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
AT01901118T 2000-01-12 2001-01-12 Pullulanase variante mit vorbestimmten eigenschaften und verfahren zur deren herstellung ATE407199T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200000045 2000-01-12
US09/514,599 US6350599B1 (en) 2000-01-12 2000-02-28 Pullulanase variants and methods for preparing such variants with predetermined properties

Publications (1)

Publication Number Publication Date
ATE407199T1 true ATE407199T1 (de) 2008-09-15

Family

ID=8158928

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01901118T ATE407199T1 (de) 2000-01-12 2001-01-12 Pullulanase variante mit vorbestimmten eigenschaften und verfahren zur deren herstellung

Country Status (5)

Country Link
US (4) US6350599B1 (de)
EP (1) EP2042593A3 (de)
AR (1) AR028198A1 (de)
AT (1) ATE407199T1 (de)
DE (1) DE60135631D1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265197B1 (en) * 1998-07-02 2001-07-24 Novozymes A/S Krogshoejvej Starch debranching enzymes
JP2007521823A (ja) 2004-02-13 2007-08-09 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
HUE056192T2 (hu) 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok
WO2016106013A1 (en) 2014-12-23 2016-06-30 E. I. Du Pont De Nemours And Company Enzymatically produced cellulose
CN107532155B (zh) 2015-02-04 2019-06-25 南京百斯杰生物工程有限公司 截短普鲁兰酶及其生产方法和应用方法
CN113667662A (zh) * 2021-07-05 2021-11-19 南京纽田科技有限公司 高性能淀粉脱支酶嵌合体菌种构建及其生产方法和应用
CN114934035B (zh) * 2022-04-06 2023-03-28 江西省科学院微生物研究所(江西省流域生态研究所) 一种淀粉降解能力提高的嗜热酸性iii型普鲁兰水解酶突变体及其制备方法和应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335208A (en) 1980-03-11 1982-06-15 Novo Industri A/S Saccharification of starch hydrolysates
US4335206A (en) * 1981-02-19 1982-06-15 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Apparatus and process for microbial detection and enumeration
US4560651A (en) * 1981-04-20 1985-12-24 Novo Industri A/S Debranching enzyme product, preparation and use thereof
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
ATE118545T1 (de) 1990-05-09 1995-03-15 Novo Nordisk As Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
ES2121786T3 (es) 1990-09-13 1998-12-16 Novo Nordisk As Variantes de lipasa.
US5231017A (en) 1991-05-17 1993-07-27 Solvay Enzymes, Inc. Process for producing ethanol
JPH0723783A (ja) 1991-07-15 1995-01-27 Q P Corp 耐熱性プルラナーゼ遺伝子ｄｎａ、これを含むプラスミドおよび微生物
DE69325981T2 (de) 1992-12-28 2000-02-03 Genencor Int Pullulanase, Mikroorganismen die sie produzieren, Verfahren zur Herstellung und ihre Anwendung
US5965442A (en) * 1993-11-12 1999-10-12 Nec Corporation Method of altering enzymes and a novel neopullulanase
JP4047379B2 (ja) 1995-02-03 2008-02-13 ノボザイムス アクティーゼルスカブ 予め決められた性質を有するα−アミラーゼ変異体のデザイン方法
KR19980702782A (ko) 1995-03-09 1998-08-05 혼 마가렛 에이. 녹말 액화 방법
JP3025625B2 (ja) 1995-05-10 2000-03-27 花王株式会社 アルカリα−アミラーゼ活性を有するアルカリプルラナーゼ遺伝子
AU6655196A (en) 1995-08-11 1997-03-12 Novo Nordisk A/S Novel lipolytic enzymes
JPH09271385A (ja) * 1996-04-01 1997-10-21 Amano Pharmaceut Co Ltd 新規ｄｎａ断片、これらを保有するプラスミド及び組換え微生物並びにこれを用いる枝切り酵素の製造法
EP0977833A1 (de) * 1997-02-28 2000-02-09 Novo Nordisk A/S Laccasemutanten.
BR9815520A (pt) 1997-05-06 2001-07-31 Du Pont Fragmento de ácido nucleico isolado, gene quimérico, célula hospedeira transformada, método de alteração do nìvel de expressão de uma pululanase de milho, método de obtenção de um fragmento de ácido nucleico e produto
EP1002061A1 (de) * 1997-07-04 2000-05-24 Novo Nordisk A/S Varianten der 6 endo-1,4-beta-glukanase und diese enthaldende reinigungszusammensetzungen
CN1163597C (zh) 1997-10-30 2004-08-25 诺维信公司 α-淀粉酶突变体
US6300115B1 (en) * 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
AU4897199A (en) 1998-07-02 2000-01-24 Novozymes A/S Starch debranching enzymes
US6265197B1 (en) * 1998-07-02 2001-07-24 Novozymes A/S Krogshoejvej Starch debranching enzymes

Also Published As

Publication number Publication date
AR028198A1 (es) 2003-04-30
EP2042593A3 (de) 2009-06-24
US20090075356A1 (en) 2009-03-19
EP2042593A2 (de) 2009-04-01
US7906306B2 (en) 2011-03-15
US20110129877A1 (en) 2011-06-02
US6350599B1 (en) 2002-02-26
US20040048247A1 (en) 2004-03-11
DE60135631D1 (de) 2008-10-16

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