ATE364433T1 - Verfahren zur erfassung von sequenzänderungen in makromoleküle durch temperaturgradienten - Google Patents

Verfahren zur erfassung von sequenzänderungen in makromoleküle durch temperaturgradienten

Info

Publication number
ATE364433T1
ATE364433T1 AT02255175T AT02255175T ATE364433T1 AT E364433 T1 ATE364433 T1 AT E364433T1 AT 02255175 T AT02255175 T AT 02255175T AT 02255175 T AT02255175 T AT 02255175T AT E364433 T1 ATE364433 T1 AT E364433T1
Authority
AT
Austria
Prior art keywords
macromolecules
temperature gradients
sequence changes
detecting sequence
difference
Prior art date
Application number
AT02255175T
Other languages
English (en)
Inventor
Reinhold E Gerber
Richard G Hatch
Original Assignee
Varian Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Varian Inc filed Critical Varian Inc
Application granted granted Critical
Publication of ATE364433T1 publication Critical patent/ATE364433T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/161—Temperature conditioning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/366—Ion-pair, e.g. ion-pair reversed phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT02255175T 2001-08-01 2002-07-24 Verfahren zur erfassung von sequenzänderungen in makromoleküle durch temperaturgradienten ATE364433T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/921,267 US6486309B1 (en) 2001-08-01 2001-08-01 Method of detecting sequence variations in macromolecules by thermal gradiant

Publications (1)

Publication Number Publication Date
ATE364433T1 true ATE364433T1 (de) 2007-07-15

Family

ID=25445180

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02255175T ATE364433T1 (de) 2001-08-01 2002-07-24 Verfahren zur erfassung von sequenzänderungen in makromoleküle durch temperaturgradienten

Country Status (4)

Country Link
US (1) US6486309B1 (de)
EP (1) EP1281422B1 (de)
AT (1) ATE364433T1 (de)
DE (1) DE60220598D1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061410A1 (en) * 2000-09-01 2002-08-08 Spectrumedix Corporation System and method for temperature gradient capillary electrophoresis
CA2425413A1 (en) * 2000-10-11 2002-04-18 Spectrumedix Corporation System and method for determining the presence of methylated cytosines in polynucleotides
EP1451372A1 (de) * 2001-10-18 2004-09-01 Spectrumedix Corporation System und verfahren zur kapillarelektrophorese mit temperaturgradienten
US6872530B2 (en) * 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
WO2004007752A2 (en) * 2002-07-15 2004-01-22 Spectrumedix Llc System and method for determining known dna variants with temperature gradient electrophoresis
US20040178070A1 (en) * 2002-07-16 2004-09-16 Zhaowei Liu Method and system for comparative genomics for organisms using temperature gradient electrophoresis
US7125492B2 (en) * 2003-07-17 2006-10-24 Agilent Technologies, Inc. Additives for reversed-phase HPLC mobile phases
US7303879B2 (en) * 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
WO2005019801A2 (en) * 2003-08-20 2005-03-03 Isis Pharmaceuticals, Inc. Method for rapid identification of molecules with functional activity towards biomolecular targets
US20120171773A1 (en) * 2009-05-29 2012-07-05 Waters Technologies Corporation Temperature Control Of Enrichment And Separation Columns In Chromatography

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795976A (en) 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
CA2308314A1 (en) * 1997-10-30 1999-05-14 Transgenomic, Inc. Method for high resolution liquid chromatographic separation of polynucleotides
US6265226B1 (en) * 1998-04-03 2001-07-24 Symyx Technologies, Inc. Automated sampling methods for rapid characterization of polymers
AU2001245650A1 (en) * 2000-03-10 2001-09-24 Ana-Gen Technologies, Inc. Mutation detection using denaturing gradients

Also Published As

Publication number Publication date
EP1281422B1 (de) 2007-06-13
DE60220598D1 (de) 2007-07-26
EP1281422A1 (de) 2003-02-05
US6486309B1 (en) 2002-11-26

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Legal Events

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