ATE354653T1 - Verfahren zur ortspezifischen massenmutagenese - Google Patents

Verfahren zur ortspezifischen massenmutagenese

Info

Publication number
ATE354653T1
ATE354653T1 AT01965350T AT01965350T ATE354653T1 AT E354653 T1 ATE354653 T1 AT E354653T1 AT 01965350 T AT01965350 T AT 01965350T AT 01965350 T AT01965350 T AT 01965350T AT E354653 T1 ATE354653 T1 AT E354653T1
Authority
AT
Austria
Prior art keywords
mutagenesis
site
specific mass
concerns
directed
Prior art date
Application number
AT01965350T
Other languages
English (en)
Inventor
Marc Delcourt
Stephane Blesa
Original Assignee
Biomethodes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes filed Critical Biomethodes
Application granted granted Critical
Publication of ATE354653T1 publication Critical patent/ATE354653T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
AT01965350T 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese ATE354653T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0010962A FR2813314B1 (fr) 2000-08-25 2000-08-25 Procede de mutagenese dirigee massive

Publications (1)

Publication Number Publication Date
ATE354653T1 true ATE354653T1 (de) 2007-03-15

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01965350T ATE354653T1 (de) 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese

Country Status (11)

Country Link
US (1) US7202086B2 (de)
EP (1) EP1311670B1 (de)
JP (1) JP5068917B2 (de)
AT (1) ATE354653T1 (de)
AU (1) AU2001286009A1 (de)
CA (1) CA2420721C (de)
DE (1) DE60126786T2 (de)
DK (1) DK1311670T3 (de)
ES (1) ES2282285T3 (de)
FR (1) FR2813314B1 (de)
WO (1) WO2002016606A2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
DE60234159D1 (de) * 2001-02-02 2009-12-10 Large Scale Biology Corp Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
CA2498319A1 (en) 2002-09-09 2004-03-18 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
DE602004020570D1 (de) * 2003-12-18 2009-05-28 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
FR2883885B1 (fr) * 2005-04-05 2010-12-10 Biomethodes Recombinaison in vitro circulaire
FR2886943B1 (fr) 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (fr) * 2006-02-03 2008-04-18 Biomethodes Sa Kanamycine kinase ii amelioree et utilisations de celle-ci
WO2007101949A2 (fr) * 2006-03-08 2007-09-13 Biomethodes Variants de l'interferon- gamma humain (infgamma)
EP3031919B1 (de) * 2007-07-31 2018-09-19 BASF Enzymes LLC Massgeschneiderte kombinatorische mehrfachstellen-konstruktion
EP2931749B1 (de) 2012-12-17 2019-04-24 Laboratoire Francais du Fractionnement et des Biotechnologies Societe Anonyme Verwendung monoklonaler antikörper zur behandlung von entzündungen und bakteriellen infektionen
WO2015011285A2 (en) 2013-07-26 2015-01-29 Biomethodes Novel variant trichoderma reesei endoglucanases
WO2019126211A1 (en) * 2017-12-20 2019-06-27 Basf Se Gene site saturation mutagenesis (gssm) method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
EP0527809B1 (de) * 1990-04-05 1995-08-16 CREA, Roberto "walk-through"-mutagenese
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6620597B1 (en) * 1998-01-09 2003-09-16 University Of Utah Research Foundation Method for in vitro amplification of circular DNA
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Also Published As

Publication number Publication date
WO2002016606A3 (fr) 2002-06-27
EP1311670B1 (de) 2007-02-21
DK1311670T3 (da) 2007-06-18
DE60126786D1 (de) 2007-04-05
US20040048268A1 (en) 2004-03-11
WO2002016606A2 (fr) 2002-02-28
FR2813314A1 (fr) 2002-03-01
CA2420721C (fr) 2013-11-12
JP5068917B2 (ja) 2012-11-07
US7202086B2 (en) 2007-04-10
AU2001286009A1 (en) 2002-03-04
JP2004507243A (ja) 2004-03-11
FR2813314B1 (fr) 2004-05-07
EP1311670A2 (de) 2003-05-21
DE60126786T2 (de) 2007-11-08
ES2282285T3 (es) 2007-10-16
CA2420721A1 (fr) 2002-02-28

Similar Documents

Publication Publication Date Title
ATE354653T1 (de) Verfahren zur ortspezifischen massenmutagenese
ATE535141T1 (de) Verfahren zur modifikation von pflanzlicher biomasse
TW200515878A (en) Flavored smokeless tobacco and methods of making
MXPA03005573A (es) Inyector y metodo para la introduccion prolongada de reactivos dentro del plasma.
SE0300924D0 (sv) A method to provide a triple well in an epitaxially based CMOS or BiCMOS process
AU2001248175A1 (en) Method and product for improved fossil fuel combustion
AU2001269087A1 (en) Method for selecting particles
IL175728A0 (en) Sesquiterpene synthases from patchouli
AP2004003033A0 (en) Method and composition for improving fuel combustion.
ATE288758T1 (de) Verfahren zur herstellung von polymorphen des clarithromycins
DE60213803D1 (en) Happier mapping
HK1071698A1 (en) Autologous t-cell vaccines materials and method
DE502005007004D1 (de) Hartkern-mantelgeschoss mit leuchtsatz und verfahren zu seiner herstellung
EA200600459A1 (ru) Способ получения цитотоксических лимфоцитов
HK1056292A1 (en) Method of culturing zooplankton, apparatus for culturing by the same, and culture obtained by the same.
EP1550736A4 (de) Aufgekohltes und abgeschrecktes element und verfahren zu seiner herstellung
MY141859A (en) Method for producing gamma-carboxylated proteins
ATE361334T1 (de) Verfahren zur herstellung von hochreinem polycarbonat
ATE363490T1 (de) Methode zur produktion monoklonaler antikörper
UA83203C2 (uk) Тіазол-(бі)циклоалкілкарбоксаніліди, засіб на їх основі та застосування для боротьби з небажаними мікроорганізмами
AU2003247599A1 (en) Methods of modulating cytokinin related processes in a plant using b3 domain proteins
DE60042424D1 (de) Majonäse-ähnliche lebensmittel sowie verfahren zur herstellung derselben
WO2002061611A3 (de) Verfahren zur darstellung von einem internetobjekt
ATE422976T1 (de) Befestigungseinsätze mit selektiv entfernbaren zapfen
ATE522604T1 (de) Verfahren zur herstellung von proteinen durch elektropulsierung

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1311670

Country of ref document: EP