AU2001286009A1 - Method for massive directed mutagenesis - Google Patents
Method for massive directed mutagenesisInfo
- Publication number
- AU2001286009A1 AU2001286009A1 AU2001286009A AU8600901A AU2001286009A1 AU 2001286009 A1 AU2001286009 A1 AU 2001286009A1 AU 2001286009 A AU2001286009 A AU 2001286009A AU 8600901 A AU8600901 A AU 8600901A AU 2001286009 A1 AU2001286009 A1 AU 2001286009A1
- Authority
- AU
- Australia
- Prior art keywords
- massive
- directed mutagenesis
- mutagenesis
- concerns
- directed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000002703 mutagenesis Methods 0.000 title abstract 4
- 231100000350 mutagenesis Toxicity 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000015572 biosynthetic process Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Ecology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Gyroscopes (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The invention concerns the field of molecular biology and more particularly that of mutagenesis. It concerns a method of high-rate directed mutagenesis, that is the formation of numerous directed mutants in reduced time and with reduced number of steps. Said method is therefore referred to as massive mutagenesis.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0010962A FR2813314B1 (en) | 2000-08-25 | 2000-08-25 | MASSIVE DIRECTED MUTAGENESIS PROCESS |
FR0010962 | 2000-08-25 | ||
PCT/FR2001/002666 WO2002016606A2 (en) | 2000-08-25 | 2001-08-24 | Method for massive directed mutagenesis |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001286009A1 true AU2001286009A1 (en) | 2002-03-04 |
Family
ID=8853747
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001286009A Abandoned AU2001286009A1 (en) | 2000-08-25 | 2001-08-24 | Method for massive directed mutagenesis |
Country Status (11)
Country | Link |
---|---|
US (1) | US7202086B2 (en) |
EP (1) | EP1311670B1 (en) |
JP (1) | JP5068917B2 (en) |
AT (1) | ATE354653T1 (en) |
AU (1) | AU2001286009A1 (en) |
CA (1) | CA2420721C (en) |
DE (1) | DE60126786T2 (en) |
DK (1) | DK1311670T3 (en) |
ES (1) | ES2282285T3 (en) |
FR (1) | FR2813314B1 (en) |
WO (1) | WO2002016606A2 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US7838219B2 (en) * | 2001-02-02 | 2010-11-23 | Novici Biotech Llc | Method of increasing complementarity in a heteroduplex |
US20050153283A1 (en) * | 2002-08-08 | 2005-07-14 | Padgett Hal S. | Method of increasing complementarity in a heteroduplex |
US20040142433A1 (en) * | 2001-02-02 | 2004-07-22 | Padgett Hal S. | Polynucleotide sequence variants |
EP1358322B1 (en) * | 2001-02-02 | 2009-10-28 | Large Scale Biology Corporation | A method of increasing complementarity in a heteroduplex polynucleotide |
US7582423B2 (en) | 2001-02-02 | 2009-09-01 | Novici Biotech Llc | Population of polynucleotide sequence variants |
FR2823219B1 (en) * | 2001-04-10 | 2003-07-04 | Pasteur Institut | MUTANTS OF DESOXYCYTIDINE KINASE WITH ENLARGED ENZYMATIC ACTIVITY |
US7647184B2 (en) | 2001-08-27 | 2010-01-12 | Hanall Pharmaceuticals, Co. Ltd | High throughput directed evolution by rational mutagenesis |
US7078211B2 (en) | 2002-02-01 | 2006-07-18 | Large Scale Biology Corporation | Nucleic acid molecules encoding endonucleases and methods of use thereof |
DE60332358D1 (en) | 2002-09-09 | 2010-06-10 | Hanall Pharmaceutical Co Ltd | PROTEASE-RESISTANT MODIFIED INTERFERON ALPHA POLYPEPTIDE |
EP1544296B8 (en) * | 2003-12-18 | 2009-08-05 | Biomethodes | Method for massive directed mutagenesis |
FR2883885B1 (en) * | 2005-04-05 | 2010-12-10 | Biomethodes | RECOMBINATION IN VITRO CIRCULAR |
FR2886943B1 (en) | 2005-06-10 | 2007-09-07 | Biomethodes Sa | METHOD OF SELECTING STABLE PROTEINS UNDER STANDARD PHYSICO-CHEMICAL CONDITIONS |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
FR2897068B1 (en) * | 2006-02-03 | 2008-04-18 | Biomethodes Sa | IMPROVED KANAMYCINE KINASE II AND USES THEREOF |
US20100260704A1 (en) * | 2006-03-08 | 2010-10-14 | Biomethodes | Human interferon-gamma (infgamma) variants |
MX353478B (en) * | 2007-07-31 | 2018-01-15 | Bp Corp North America Inc Star | Tailored multi-site combinatorial assembly. |
EP3514175A1 (en) | 2012-12-17 | 2019-07-24 | Laboratoire Français du Fractionnement et des Biotechnologies | Use of monoclonal antibodies for the treatment of inflammation and bacterial infections |
WO2015011285A2 (en) | 2013-07-26 | 2015-01-29 | Biomethodes | Novel variant trichoderma reesei endoglucanases |
WO2019126211A1 (en) * | 2017-12-20 | 2019-06-27 | Basf Se | Gene site saturation mutagenesis (gssm) method |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4960707A (en) * | 1987-08-17 | 1990-10-02 | Associated Universities, Inc. | Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae |
ES2078518T3 (en) * | 1990-04-05 | 1995-12-16 | Roberto Crea | COMPLETE DISPLACEMENT MUTAGENESIS. |
WO1993020096A1 (en) * | 1991-05-08 | 1993-10-14 | Stratagene | Oligonucleotide libraries useful for producing primers |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5830696A (en) * | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
DE69934088T2 (en) * | 1998-01-09 | 2007-06-21 | University Of Utah Research Foundation, Salt Lake City | METHOD FOR THE IN VITRO AMPLIFICATION OF CIRCULAR DNA |
US6368861B1 (en) * | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
-
2000
- 2000-08-25 FR FR0010962A patent/FR2813314B1/en not_active Expired - Lifetime
-
2001
- 2001-08-24 EP EP01965350A patent/EP1311670B1/en not_active Expired - Lifetime
- 2001-08-24 ES ES01965350T patent/ES2282285T3/en not_active Expired - Lifetime
- 2001-08-24 JP JP2002522277A patent/JP5068917B2/en not_active Expired - Fee Related
- 2001-08-24 DK DK01965350T patent/DK1311670T3/en active
- 2001-08-24 CA CA2420721A patent/CA2420721C/en not_active Expired - Fee Related
- 2001-08-24 WO PCT/FR2001/002666 patent/WO2002016606A2/en active IP Right Grant
- 2001-08-24 AT AT01965350T patent/ATE354653T1/en active
- 2001-08-24 US US10/362,576 patent/US7202086B2/en not_active Expired - Fee Related
- 2001-08-24 AU AU2001286009A patent/AU2001286009A1/en not_active Abandoned
- 2001-08-24 DE DE60126786T patent/DE60126786T2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60126786D1 (en) | 2007-04-05 |
FR2813314B1 (en) | 2004-05-07 |
FR2813314A1 (en) | 2002-03-01 |
JP5068917B2 (en) | 2012-11-07 |
WO2002016606A2 (en) | 2002-02-28 |
JP2004507243A (en) | 2004-03-11 |
DE60126786T2 (en) | 2007-11-08 |
WO2002016606A3 (en) | 2002-06-27 |
ATE354653T1 (en) | 2007-03-15 |
EP1311670A2 (en) | 2003-05-21 |
US7202086B2 (en) | 2007-04-10 |
ES2282285T3 (en) | 2007-10-16 |
US20040048268A1 (en) | 2004-03-11 |
DK1311670T3 (en) | 2007-06-18 |
CA2420721C (en) | 2013-11-12 |
EP1311670B1 (en) | 2007-02-21 |
CA2420721A1 (en) | 2002-02-28 |
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