ATE341645T1 - Verfahren für die reverse transkription - Google Patents

Verfahren für die reverse transkription

Info

Publication number
ATE341645T1
ATE341645T1 AT98918516T AT98918516T ATE341645T1 AT E341645 T1 ATE341645 T1 AT E341645T1 AT 98918516 T AT98918516 T AT 98918516T AT 98918516 T AT98918516 T AT 98918516T AT E341645 T1 ATE341645 T1 AT E341645T1
Authority
AT
Austria
Prior art keywords
reverse transcription
dependent
synthesis
improving
full
Prior art date
Application number
AT98918516T
Other languages
English (en)
Inventor
Jerome J Jendrisak
Original Assignee
Epict Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epict Technologies Corp filed Critical Epict Technologies Corp
Application granted granted Critical
Publication of ATE341645T1 publication Critical patent/ATE341645T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gyroscopes (AREA)
  • Photovoltaic Devices (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
AT98918516T 1997-04-21 1998-04-15 Verfahren für die reverse transkription ATE341645T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/840,474 US6030814A (en) 1997-04-21 1997-04-21 Reverse transcription method

Publications (1)

Publication Number Publication Date
ATE341645T1 true ATE341645T1 (de) 2006-10-15

Family

ID=25282475

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98918516T ATE341645T1 (de) 1997-04-21 1998-04-15 Verfahren für die reverse transkription

Country Status (9)

Country Link
US (1) US6030814A (de)
EP (1) EP0977891B1 (de)
JP (1) JP2000513585A (de)
CN (1) CN1261406A (de)
AT (1) ATE341645T1 (de)
AU (1) AU743907B2 (de)
CA (1) CA2291855C (de)
DE (1) DE69836076T2 (de)
WO (1) WO1998048053A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19920611A1 (de) * 1999-05-05 2000-11-09 Roche Diagnostics Gmbh Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
EP1436385A4 (de) * 2001-09-14 2005-12-14 Invitrogen Corp Dna-polymerasen und mutanten davon
WO2004048594A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
DK2374900T3 (en) * 2003-03-07 2016-10-17 Rubicon Genomics Inc Polynucleotides for amplification and analysis of the total genomic and total transcription libraries generated by a DNA polymerization
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
TW200506052A (en) * 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
AU2006287548A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
WO2008013885A2 (en) * 2006-07-25 2008-01-31 Stratagene California Zwitterionic detergents for the storage and use of dna polymerases
CA2707436C (en) 2007-06-29 2014-01-28 Epicentre Technologies Corporation Copy dna and sense rna
US20120283111A1 (en) 2011-05-02 2012-11-08 Pioneer Hi-Bred International, Inc. Bacterial mRNA screen strategy for novel pesticide-encoding nucleic acid molecule discovery
CN113234802A (zh) * 2021-06-04 2021-08-10 翌圣生物科技(上海)股份有限公司 泊洛沙姆在提高逆转录酶效率或提高逆转录酶抑制剂耐受性中的应用及添加剂混合物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5322770A (en) * 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
CA2071213C (en) * 1989-12-22 2000-05-02 David H. Gelfand High temperature reverse transcriptases
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5545539A (en) * 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification

Also Published As

Publication number Publication date
CA2291855A1 (en) 1998-10-29
DE69836076T2 (de) 2007-04-19
EP0977891B1 (de) 2006-10-04
AU7142398A (en) 1998-11-13
US6030814A (en) 2000-02-29
AU743907B2 (en) 2002-02-07
WO1998048053A1 (en) 1998-10-29
CA2291855C (en) 2005-01-11
CN1261406A (zh) 2000-07-26
EP0977891A1 (de) 2000-02-09
JP2000513585A (ja) 2000-10-17
DE69836076D1 (de) 2006-11-16

Similar Documents

Publication Publication Date Title
ATE341645T1 (de) Verfahren für die reverse transkription
ATE370254T1 (de) Verfahren zur herstellung hochreinen tantals für zerstäubungstargets
MXPA04001127A (es) Ensayo de enlazamiento competitivo para la identificacion de inhibidores de polimerasa vhc.
AU1408900A (en) Method for synthesizing cdna
ATE452888T1 (de) Trans-9,10-dehydroepothilon c und d, analoga davon und verfahren zu deren herstellung
DE50303174D1 (de) Verfahren und Vorrichtung zur Herstellung von fliessfähigem Metallschaum
DE68911215T2 (de) Verfahren zur Herstellung von hochantikorrosiven, oberflächenbehandelten Stahlplatten.
DE60018902D1 (de) Verfahren zur herstellung von nitroxymethylphenylestern von aspirinderivaten
DE60227335D1 (de) Kontinuierliches Verfahren zur Modifizierung von Stärke und Stärkederivate mittels Verzweigungsenzyme
NL185635B (nl) Werkwijze voor de vervaardiging van een geluidsstaaf voor percussie-muziekinstrumenten.
DE502004001985D1 (de) Verfahren zur Herstellung von expandiertem Graphit
MXPA02011762A (es) Proceso para la preparacion de compuestos metales de transicion y su empleo para la polimerizacion de olefinas.
ATE217551T1 (de) Verfahren zum herstellen von nickel-titan- hohlprofilen
DE60106057D1 (de) Verfahren zur herstellung von baccatin iii-derivativen
ATE417131T1 (de) Verfahren zur herstellung von metallschaumkörpern auf basis von zink
ATE359252T1 (de) Verfahren zur herstellung von olefinen
DE60011463D1 (de) Verfahren zur herstellung von 24(s)-hydroxyvitamin d2
PT1064265E (pt) Sintese de 3-amino-2-cloro-4-metilpiridina a partir de malononitrilo e acetona
ATE347609T1 (de) Enzymatisches verfahren zur herstellung von organofluorverbindungen
DE60014330D1 (de) Verfahren zur herstellung von 1,4,7,10-tetraazacyclododecan
DE69735131D1 (de) Für die gdp-fucose-pyrophosphorylase kodierende nukleinsäure
MXPA04004526A (es) N-metil-homocisteinas, su uso y metodo para su produccion.
DE69416325D1 (de) Enzymatisches verfahren zur herstellung von morphin-6-glucuroniden oder substituierten morphin-6-glucuroniden
ID24516A (id) Proses penyalutan elastomer-elastomer silikon
WO2003016483A3 (en) METHOD OF LABELLING cRNAs FOR PROBING OLIGO-BASED MICROARRAYS

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties