ATE341645T1 - Verfahren für die reverse transkription - Google Patents

Verfahren für die reverse transkription

Info

Publication number
ATE341645T1
ATE341645T1 AT98918516T AT98918516T ATE341645T1 AT E341645 T1 ATE341645 T1 AT E341645T1 AT 98918516 T AT98918516 T AT 98918516T AT 98918516 T AT98918516 T AT 98918516T AT E341645 T1 ATE341645 T1 AT E341645T1
Authority
AT
Austria
Prior art keywords
reverse transcription
dependent
synthesis
improving
full
Prior art date
Application number
AT98918516T
Other languages
English (en)
Inventor
Jerome J Jendrisak
Original Assignee
Epict Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epict Technologies Corp filed Critical Epict Technologies Corp
Application granted granted Critical
Publication of ATE341645T1 publication Critical patent/ATE341645T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gyroscopes (AREA)
  • Photovoltaic Devices (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
AT98918516T 1997-04-21 1998-04-15 Verfahren für die reverse transkription ATE341645T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/840,474 US6030814A (en) 1997-04-21 1997-04-21 Reverse transcription method

Publications (1)

Publication Number Publication Date
ATE341645T1 true ATE341645T1 (de) 2006-10-15

Family

ID=25282475

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98918516T ATE341645T1 (de) 1997-04-21 1998-04-15 Verfahren für die reverse transkription

Country Status (9)

Country Link
US (1) US6030814A (de)
EP (1) EP0977891B1 (de)
JP (1) JP2000513585A (de)
CN (1) CN1261406A (de)
AT (1) ATE341645T1 (de)
AU (1) AU743907B2 (de)
CA (1) CA2291855C (de)
DE (1) DE69836076T2 (de)
WO (1) WO1998048053A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19920611A1 (de) * 1999-05-05 2000-11-09 Roche Diagnostics Gmbh Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
EP1576188B1 (de) 2002-11-21 2008-10-15 Epicentre Technologies Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
DE602004029560D1 (de) * 2003-03-07 2010-11-25 Rubicon Genomics Inc Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden
TW200506052A (en) * 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
JP2009506788A (ja) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド 核酸の等温増幅のための方法、組成物及びキット
US20080064071A1 (en) * 2006-07-25 2008-03-13 Hogrefe Holly H Zwitterionic detergents for the storage and use of DNA polymerases
WO2009006438A2 (en) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Copy dna and sense rna
EP2705153A1 (de) 2011-05-02 2014-03-12 Pioneer Hi-Bred International, Inc. Screening von bakterieller mrna für die entdeckung neuer pestizid-kodierender nukleinsäuremoleküle
CN113234802A (zh) * 2021-06-04 2021-08-10 翌圣生物科技(上海)股份有限公司 泊洛沙姆在提高逆转录酶效率或提高逆转录酶抑制剂耐受性中的应用及添加剂混合物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5322770A (en) * 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
DE69030386T2 (de) * 1989-12-22 1997-10-09 F. Hoffmann-La Roche Ag, Basel Bei hoher temperatur aktive reverse transkriptasen
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5545539A (en) * 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification

Also Published As

Publication number Publication date
AU7142398A (en) 1998-11-13
EP0977891B1 (de) 2006-10-04
JP2000513585A (ja) 2000-10-17
US6030814A (en) 2000-02-29
AU743907B2 (en) 2002-02-07
EP0977891A1 (de) 2000-02-09
WO1998048053A1 (en) 1998-10-29
DE69836076T2 (de) 2007-04-19
CN1261406A (zh) 2000-07-26
DE69836076D1 (de) 2006-11-16
CA2291855C (en) 2005-01-11
CA2291855A1 (en) 1998-10-29

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Legal Events

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