ATE280773T1 - Verfahren zur befestigung von oligonukleotiden auf ein substrat - Google Patents
Verfahren zur befestigung von oligonukleotiden auf ein substratInfo
- Publication number
- ATE280773T1 ATE280773T1 AT00955877T AT00955877T ATE280773T1 AT E280773 T1 ATE280773 T1 AT E280773T1 AT 00955877 T AT00955877 T AT 00955877T AT 00955877 T AT00955877 T AT 00955877T AT E280773 T1 ATE280773 T1 AT E280773T1
- Authority
- AT
- Austria
- Prior art keywords
- substrate
- oligonucleotide primer
- containing multiple
- glass
- glass substrate
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 8
- 239000000758 substrate Substances 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 239000011521 glass Substances 0.000 abstract 6
- JUIKUQOUMZUFQT-UHFFFAOYSA-N 2-bromoacetamide Chemical compound NC(=O)CBr JUIKUQOUMZUFQT-UHFFFAOYSA-N 0.000 abstract 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 abstract 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 abstract 2
- 229910000077 silane Inorganic materials 0.000 abstract 2
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 abstract 1
- 125000004103 aminoalkyl group Chemical group 0.000 abstract 1
- 238000004873 anchoring Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 125000002228 disulfide group Chemical group 0.000 abstract 1
- 229920000592 inorganic polymer Polymers 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 238000005935 nucleophilic addition reaction Methods 0.000 abstract 1
- 229920000620 organic polymer Polymers 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 125000003396 thiol group Chemical group [H]S* 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Structural Engineering (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/388,702 US6528319B1 (en) | 1999-09-02 | 1999-09-02 | Method for anchoring oligonucleotides to a substrate |
| PCT/US2000/023255 WO2001016152A2 (en) | 1999-09-02 | 2000-08-24 | Method for anchoring oligonucleotides to a substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE280773T1 true ATE280773T1 (de) | 2004-11-15 |
Family
ID=23535172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00955877T ATE280773T1 (de) | 1999-09-02 | 2000-08-24 | Verfahren zur befestigung von oligonukleotiden auf ein substrat |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6528319B1 (de) |
| EP (1) | EP1210356B1 (de) |
| AT (1) | ATE280773T1 (de) |
| AU (1) | AU6800400A (de) |
| CA (1) | CA2381761A1 (de) |
| DE (1) | DE60015368T2 (de) |
| WO (1) | WO2001016152A2 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020076832A1 (en) * | 2000-06-02 | 2002-06-20 | Pirrung Michael C. | Method of attaching a biopolymer to a solid support |
| WO2003029494A1 (en) * | 2001-10-03 | 2003-04-10 | Micrologix Biotech Inc. | Attachment of thiophosphate tethered oligonucleotides to a solid surface |
| AU2002363537A1 (en) * | 2001-11-07 | 2003-05-19 | Integrigen, Inc. | Method for generation of modular polynucleotides using solid supports |
| DE10159904A1 (de) * | 2001-12-06 | 2003-07-03 | Adnagen Ag | Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür |
| US20050074781A1 (en) * | 2003-10-02 | 2005-04-07 | Herbert von Schroeder | Nucleic acid braided J-probes |
| GB0326073D0 (en) | 2003-11-07 | 2003-12-10 | Solexa Ltd | Improvements in or relating to polynucleotide arrays |
| US7781187B2 (en) | 2005-12-30 | 2010-08-24 | Corning Incorporated | Fluorescent dyes |
| KR101556798B1 (ko) | 2006-10-05 | 2015-10-01 | 메사츄세츠 인스티튜트 어브 테크놀로지 | 고성능 분석을 위한 다중기능성 인코딩된 입자 |
| US9476101B2 (en) * | 2010-06-07 | 2016-10-25 | Firefly Bioworks, Inc. | Scanning multifunctional particles |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| US5237016A (en) * | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
| US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
| SE501439C2 (sv) | 1993-06-22 | 1995-02-13 | Pharmacia Lkb Biotech | Sätt och anordning för analys av polynukleotidsekvenser |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5770365A (en) | 1995-08-25 | 1998-06-23 | Tm Technologies, Inc. | Nucleic acid capture moieties |
| US5770151A (en) | 1996-06-05 | 1998-06-23 | Molecular Dynamics, Inc. | High-speed liquid deposition device for biological molecule array formation |
| US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
-
1999
- 1999-09-02 US US09/388,702 patent/US6528319B1/en not_active Expired - Fee Related
-
2000
- 2000-08-24 DE DE60015368T patent/DE60015368T2/de not_active Expired - Fee Related
- 2000-08-24 AU AU68004/00A patent/AU6800400A/en not_active Abandoned
- 2000-08-24 CA CA002381761A patent/CA2381761A1/en not_active Abandoned
- 2000-08-24 AT AT00955877T patent/ATE280773T1/de not_active IP Right Cessation
- 2000-08-24 WO PCT/US2000/023255 patent/WO2001016152A2/en not_active Ceased
- 2000-08-24 EP EP00955877A patent/EP1210356B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6528319B1 (en) | 2003-03-04 |
| EP1210356A2 (de) | 2002-06-05 |
| EP1210356B1 (de) | 2004-10-27 |
| AU6800400A (en) | 2001-03-26 |
| WO2001016152A2 (en) | 2001-03-08 |
| DE60015368T2 (de) | 2006-02-02 |
| CA2381761A1 (en) | 2001-03-08 |
| DE60015368D1 (de) | 2004-12-02 |
| WO2001016152A3 (en) | 2001-07-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |