ATE267267T1 - Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion - Google Patents

Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion

Info

Publication number
ATE267267T1
ATE267267T1 AT98929076T AT98929076T ATE267267T1 AT E267267 T1 ATE267267 T1 AT E267267T1 AT 98929076 T AT98929076 T AT 98929076T AT 98929076 T AT98929076 T AT 98929076T AT E267267 T1 ATE267267 T1 AT E267267T1
Authority
AT
Austria
Prior art keywords
gene expression
chain reaction
polymerase chain
reverse transcriptase
transcriptase polymerase
Prior art date
Application number
AT98929076T
Other languages
English (en)
Inventor
James C Willey
Erin L Crawford
Jeffery P Demuth
Clara M Jackson
David A Weaver
Original Assignee
Ohio Med College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio Med College filed Critical Ohio Med College
Application granted granted Critical
Publication of ATE267267T1 publication Critical patent/ATE267267T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT98929076T 1997-06-16 1998-06-15 Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion ATE267267T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/876,766 US5876978A (en) 1993-04-06 1997-06-16 Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
PCT/US1998/012420 WO1998058083A2 (en) 1997-06-16 1998-06-15 Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction

Publications (1)

Publication Number Publication Date
ATE267267T1 true ATE267267T1 (de) 2004-06-15

Family

ID=25368530

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98929076T ATE267267T1 (de) 1997-06-16 1998-06-15 Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion

Country Status (10)

Country Link
US (1) US5876978A (de)
EP (1) EP0998581B1 (de)
JP (2) JP2001519672A (de)
CN (1) CN1152965C (de)
AT (1) ATE267267T1 (de)
AU (1) AU8072898A (de)
CA (2) CA2482307C (de)
DE (1) DE69824004T2 (de)
RU (1) RU2190019C2 (de)
WO (1) WO1998058083A2 (de)

Families Citing this family (112)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258543B1 (en) * 1996-05-02 2001-07-10 Xtrana, Inc. Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor
EP0910666B1 (de) * 1996-05-02 2006-08-09 Applera Corporation Quantifizierung von rna-transkripten mittels genomischer dna als internen standard der amplifizierungsreaktion
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US6054276A (en) * 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
DE19906169A1 (de) * 1999-02-08 2000-08-10 Bioinside Ges Fuer Biodiagnost Testkit und Verfahren zum quantitativen Nachweis von gentechnisch veränderter DNS in Lebensmitteln mittels fluoreszenzgekoppelter PCR
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6618679B2 (en) 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
EP1172445A1 (de) * 2000-07-14 2002-01-16 Praenadia GmbH Verfahren zur direkten genetischen Analyse von Zielzellen anhand Fluoreszentensonden
US6984522B2 (en) * 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
US20080194022A1 (en) * 2000-08-03 2008-08-14 Clarke Michael F Isolation and use of solid tumor stem cells
US8044259B2 (en) 2000-08-03 2011-10-25 The Regents Of The University Of Michigan Determining the capability of a test compound to affect solid tumor stem cells
CA2431170C (en) * 2000-12-12 2011-07-26 Aisin Seiki Kabushiki Kaisha Method of analyzing expression of gene
AU2002250285A1 (en) * 2001-03-14 2002-09-24 Medical College Of Ohio Method for quantitative measurement of gene expression for identifying individuals at risk for bronchogenic carcinoma
US7229774B2 (en) 2001-08-02 2007-06-12 Regents Of The University Of Michigan Expression profile of prostate cancer
EP1953244A1 (de) 2002-01-09 2008-08-06 Nakamura, Yusuke Krebsprofile
CN100516211C (zh) 2002-02-20 2009-07-22 希森美康株式会社 用于持家基因mRNA检测的核酸扩增用引物和使用该引物的检查方法
US20030186246A1 (en) * 2002-03-28 2003-10-02 Willey James C. Multiplex standardized reverse transcriptase-polymerase chain reacton method for assessment of gene expression in small biological samples
US8722331B2 (en) * 2002-03-28 2014-05-13 University Of Toledo Method for selecting a treatment for non-small cell lung cancer using gene expression profiles
CN1182256C (zh) * 2002-08-09 2004-12-29 周国华 基因表达量比较分析法
EP3000899A1 (de) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
CN1316036C (zh) * 2003-01-06 2007-05-16 徐定邦 一种以聚合酶链式反应为基础的基因定量方法
AU2004256425A1 (en) * 2003-06-09 2005-01-20 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
CA2528572C (en) 2003-06-10 2020-08-25 The Trustees Of Boston University Gene expression analysis of airway epithelial cells for diagnosing lung cancer
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
US7332280B2 (en) * 2003-10-14 2008-02-19 Ronald Levy Classification of patients having diffuse large B-cell lymphoma based upon gene expression
CA2554779A1 (en) * 2004-02-03 2005-08-18 The Regents Of The University Of Michigan Compositions and methods for characterizing, regulating, diagnosing, and treating cancer
EP1721014B1 (de) * 2004-02-18 2013-07-17 Trustees Of Boston University Verfahren zum nachweis und zur quantifizierung seltener mutationen
EP2208797A3 (de) 2004-03-01 2010-11-24 Applied Biosystems, LLC Verfahren, Zusammensetzungen und Kits für Polynukleotidamplifikation
EP1753873A4 (de) 2004-03-05 2008-11-05 Ohio Med College Verfahren und zusammensetzungen zur beurteilung von nukleinsäuren und allelen
CN100460520C (zh) * 2004-04-05 2009-02-11 周国华 采用碱基序列测定法比较同一基因在不同来源中的表达量的方法
CN1294279C (zh) * 2004-04-05 2007-01-10 周国华 采用碱基序列测定法比较同一基因在不同来源中的表达量的方法
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
JP2006014723A (ja) * 2004-06-01 2006-01-19 Sumitomo Chemical Co Ltd コモンマーモセット由来のグリセルアルデヒド３リン酸デヒドロゲナーゼ遺伝子及びその利用
US7498315B2 (en) 2004-06-01 2009-03-03 Pronai Therapeutics, Inc. Methods and compositions for the inhibition of gene expression
WO2005118872A2 (en) * 2004-06-01 2005-12-15 The Regents Of The University Of Michigan Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
JP2006042804A (ja) * 2004-07-09 2006-02-16 Sumitomo Chemical Co Ltd コモンマーモセット由来の１８ｓリボソームｒｎａ遺伝子及びその利用
US20080286766A1 (en) * 2004-07-09 2008-11-20 Guohua Zhou Comparing Method for Expression Amount of the Same Gene from Different Sources by Base Sequence Measurement
AU2005280528B2 (en) * 2004-07-30 2010-12-23 Adeza Biomedical Corporation Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin
ES2530057T3 (es) * 2005-01-21 2015-02-26 Gene Express, Inc. Composiciones y procedimientos de uso de mezclas estandarizadas
EP3211093A1 (de) 2005-04-14 2017-08-30 The Trustees of Boston University Diagnose für lungenerkrankungen mittels klassenvorhersage
CN102539734B (zh) 2005-05-12 2016-02-03 清华大学 核仁素辅助的癌症诊断与治疗方法
US7439024B2 (en) * 2005-06-01 2008-10-21 The United States Of America As Represented By The Department Of Veterans Affairs Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
EP1907858A4 (de) * 2005-06-13 2009-04-08 Univ Michigan Zusammensetzungen und verfahren zur behandlung und diagnose von krebs
US20070099209A1 (en) * 2005-06-13 2007-05-03 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
US20070092892A1 (en) * 2005-09-02 2007-04-26 Willey James C Methods and compositions for identifying biomarkers useful in diagnosis and/or treatment of biological states
US8652467B2 (en) * 2005-10-14 2014-02-18 The Regents Of The University Of Michigan Dek protein compositions and methods of using the same
PL2481756T3 (pl) 2005-10-31 2018-02-28 Oncomed Pharmaceuticals, Inc. Kompozycje i sposoby diagnozowania i leczenia nowotworu
US7723477B2 (en) * 2005-10-31 2010-05-25 Oncomed Pharmaceuticals, Inc. Compositions and methods for inhibiting Wnt-dependent solid tumor cell growth
WO2007053648A2 (en) * 2005-10-31 2007-05-10 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
WO2008121102A2 (en) * 2006-02-21 2008-10-09 The Regents Of The University Of Michigan Hedgehog signaling pathway antagonist cancer treatment
CA2645310A1 (en) 2006-03-09 2007-09-13 The Trustees Of Boston University Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells
BRPI0709282B8 (pt) 2006-03-29 2021-05-25 Merial Ltd vacina contra estreptococos
WO2008030605A2 (en) * 2006-09-08 2008-03-13 The Regents Of The University Of Michigan Herv group ii viruses in lymphoma and cancer
WO2008045952A2 (en) * 2006-10-10 2008-04-17 The Regents Of The University Of Michigan Photoreceptor precursor cells
WO2008092002A2 (en) 2007-01-24 2008-07-31 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing pancreatic cancer
US10745701B2 (en) 2007-06-28 2020-08-18 The Trustees Of Princeton University Methods of identifying and treating poor-prognosis cancers
US20090324596A1 (en) 2008-06-30 2009-12-31 The Trustees Of Princeton University Methods of identifying and treating poor-prognosis cancers
EP3424529A1 (de) 2007-07-02 2019-01-09 Oncomed Pharmaceuticals, Inc. Zusammensetzungen und verfahren zur behandlung und diagnose von krebs
US8765368B2 (en) * 2007-09-17 2014-07-01 The University Of Toledo Cancer risk biomarker
CA2700200A1 (en) 2007-09-19 2009-03-26 The Trustees Of Boston University Identification of novel pathways for drug development for lung disease
RU2375458C2 (ru) * 2007-12-25 2009-12-10 Сергей Феликсович Дрозд Способ определения числа копий трансгена в образце днк
WO2009091556A2 (en) * 2008-01-17 2009-07-23 The General Hospital Corporation Diagnostic methods and kits using fibroblast growth factor-23
NZ592338A (en) 2008-09-26 2012-11-30 Oncomed Pharm Inc Frizzled-binding agents and uses thereof
EP2356462B1 (de) 2008-11-11 2017-01-04 The Regents of the University of Michigan Anti-cxcr1-zusammensetzungen und verfahren
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US8362318B2 (en) 2008-12-18 2013-01-29 Board Of Trustees Of Michigan State University Enzyme directed oil biosynthesis in microalgae
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
JP6078339B2 (ja) 2009-05-07 2017-02-08 ベラサイト インコーポレイテッド 甲状腺状態の診断のための方法および組成物
EP2963128A1 (de) 2009-07-10 2016-01-06 The Regents of the University of Michigan Zusammensetzungen und verfahren zur diagnose und behandlung von makuladegeneration
CA2768391C (en) 2009-07-21 2016-09-06 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
WO2011025540A1 (en) 2009-08-28 2011-03-03 Bellweather Farms Chemical induction of lactation
WO2011082253A2 (en) 2009-12-30 2011-07-07 Board Of Trustees Of Michigan State University A method to produce acetyldiacylglycerols (ac-tags) by expression ofan acetyltransferase gene isolated from euonymus alatus (burning bush)
TWI535445B (zh) 2010-01-12 2016-06-01 安可美德藥物股份有限公司 Ｗｎｔ拮抗劑及治療和篩選方法
US8574832B2 (en) 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
AU2011235904B2 (en) 2010-04-01 2015-10-08 Oncomed Pharmaceuticals, Inc. Frizzled-binding agents and uses thereof
JP2013523154A (ja) 2010-04-06 2013-06-17 マサチューセッツ・インスティトュート・オブ・テクノロジー 転写物測定値数が減少した、遺伝子発現プロファイリング
US9125931B2 (en) 2010-04-06 2015-09-08 Massachusetts Institute Of Technology Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers
EP2407242A1 (de) 2010-07-13 2012-01-18 Dublin City University Direkte Klonanalyse und Auswahltechnologie
CA2858526C (en) 2010-12-07 2020-06-30 Northwestern University Molecular targets for als and related disorders
US9657290B2 (en) 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
DK2909339T3 (en) * 2012-10-18 2018-08-13 Idexx Lab Inc Kits comprehensive controls for nucleic acid amplification
US9266959B2 (en) 2012-10-23 2016-02-23 Oncomed Pharmaceuticals, Inc. Methods of treating neuroendocrine tumors using frizzled-binding agents
US9944973B2 (en) 2012-11-26 2018-04-17 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
CA3043574C (en) 2012-11-26 2023-09-19 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
US9359444B2 (en) 2013-02-04 2016-06-07 Oncomed Pharmaceuticals Inc. Methods and monitoring of treatment with a Wnt pathway inhibitor
US10526655B2 (en) 2013-03-14 2020-01-07 Veracyte, Inc. Methods for evaluating COPD status
US9168300B2 (en) 2013-03-14 2015-10-27 Oncomed Pharmaceuticals, Inc. MET-binding agents and uses thereof
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
US10392629B2 (en) 2014-01-17 2019-08-27 Board Of Trustees Of Michigan State University Increased caloric and nutritional content of plant biomass
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
US20170335396A1 (en) 2014-11-05 2017-11-23 Veracyte, Inc. Systems and methods of diagnosing idiopathic pulmonary fibrosis on transbronchial biopsies using machine learning and high dimensional transcriptional data
EP3037545A1 (de) 2014-12-23 2016-06-29 The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin DNS-Methylierungstest auf Prostatakrebs
US10526600B2 (en) 2015-02-22 2020-01-07 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
WO2017201302A1 (en) 2016-05-18 2017-11-23 The University Of Chicago Btk mutation and ibrutinib resistance
US10927417B2 (en) 2016-07-08 2021-02-23 Trustees Of Boston University Gene expression-based biomarker for the detection and monitoring of bronchial premalignant lesions
WO2018089953A1 (en) 2016-11-14 2018-05-17 Orca Biosystems, Inc. Methods and apparatuses for sorting target particles
AU2018372906B2 (en) 2017-11-22 2025-04-03 The Regents Of The University Of Michigan Compositions and methods for treating cancer
US12235273B2 (en) 2018-05-04 2025-02-25 Abbott Laboratories HBV diagnostic, prognostic, and therapeutic methods and products
WO2020102513A1 (en) 2018-11-14 2020-05-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating cancer

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643765A (en) * 1993-04-06 1997-07-01 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
EP0636413B1 (de) * 1993-07-28 2001-11-14 PE Corporation (NY) Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung

Also Published As

Publication number Publication date
RU2190019C2 (ru) 2002-09-27
CA2294470A1 (en) 1998-12-23
CN1265155A (zh) 2000-08-30
EP0998581B1 (de) 2004-05-19
CA2294470C (en) 2005-09-06
WO1998058083A3 (en) 1999-03-11
EP0998581A2 (de) 2000-05-10
WO1998058083A2 (en) 1998-12-23
CN1152965C (zh) 2004-06-09
CA2482307A1 (en) 1998-12-23
US5876978A (en) 1999-03-02
DE69824004T2 (de) 2005-06-02
CA2482307C (en) 2009-05-12
JP2005095179A (ja) 2005-04-14
JP2001519672A (ja) 2001-10-23
AU8072898A (en) 1999-01-04
DE69824004D1 (de) 2004-06-24

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