ATE264921T1 - Verfahren zur auffindung von dephosphorylierung phosphorylierte threonin oder serin durch phosphataseaktivität - Google Patents

Verfahren zur auffindung von dephosphorylierung phosphorylierte threonin oder serin durch phosphataseaktivität

Info

Publication number
ATE264921T1
ATE264921T1 AT00122707T AT00122707T ATE264921T1 AT E264921 T1 ATE264921 T1 AT E264921T1 AT 00122707 T AT00122707 T AT 00122707T AT 00122707 T AT00122707 T AT 00122707T AT E264921 T1 ATE264921 T1 AT E264921T1
Authority
AT
Austria
Prior art keywords
serine
peptide
protein
phosphatase activity
dephosphorylation
Prior art date
Application number
AT00122707T
Other languages
English (en)
Inventor
Joachim Dr Kraemer
Christiane Peiker
Karsten Dr Henco
Original Assignee
Evotec Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Ag filed Critical Evotec Ag
Application granted granted Critical
Publication of ATE264921T1 publication Critical patent/ATE264921T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00122707T 2000-10-18 2000-10-18 Verfahren zur auffindung von dephosphorylierung phosphorylierte threonin oder serin durch phosphataseaktivität ATE264921T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00122707A EP1199370B1 (de) 2000-10-18 2000-10-18 Verfahren zur Auffindung von Dephosphorylierung phosphorylierte Threonin oder Serin durch Phosphataseaktivität

Publications (1)

Publication Number Publication Date
ATE264921T1 true ATE264921T1 (de) 2004-05-15

Family

ID=8170123

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00122707T ATE264921T1 (de) 2000-10-18 2000-10-18 Verfahren zur auffindung von dephosphorylierung phosphorylierte threonin oder serin durch phosphataseaktivität

Country Status (3)

Country Link
EP (1) EP1199370B1 (de)
AT (1) ATE264921T1 (de)
DE (1) DE60010086T2 (de)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010461A1 (en) * 1991-11-12 1993-05-27 Promega Corporation Non-radioactive enzyme assay
US5814507A (en) * 1996-05-24 1998-09-29 Genentech, Inc. κ/μ-like protein tyrosine phosphatase, PTP λ
EP0874052A3 (de) * 1997-04-22 1999-02-24 BIOPHARM GESELLSCHAFT ZUR BIOTECHNOLOGISCHEN ENTWICKLUNG VON PHARMAKA mbH Menschliche Proteinphosphatase kodierende Nukleinsäure
ATE233325T1 (de) * 1997-12-05 2003-03-15 Upjohn Co Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase

Also Published As

Publication number Publication date
DE60010086T2 (de) 2005-04-28
EP1199370A1 (de) 2002-04-24
EP1199370B1 (de) 2004-04-21
DE60010086D1 (de) 2004-05-27

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Legal Events

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