ATE215696T1 - Chemilumineszenz-assay mittels energietransfer - Google Patents

Chemilumineszenz-assay mittels energietransfer

Info

Publication number
ATE215696T1
ATE215696T1 AT95906704T AT95906704T ATE215696T1 AT E215696 T1 ATE215696 T1 AT E215696T1 AT 95906704 T AT95906704 T AT 95906704T AT 95906704 T AT95906704 T AT 95906704T AT E215696 T1 ATE215696 T1 AT E215696T1
Authority
AT
Austria
Prior art keywords
energy transfer
chemiluminescence assay
attophos
biopolymer
assays
Prior art date
Application number
AT95906704T
Other languages
English (en)
Inventor
Irena Bronstein
Brooks Edwards
John Voyta
Original Assignee
Tropix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tropix Inc filed Critical Tropix Inc
Application granted granted Critical
Publication of ATE215696T1 publication Critical patent/ATE215696T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D321/00—Heterocyclic compounds containing rings having two oxygen atoms as the only ring hetero atoms, not provided for by groups C07D317/00 - C07D319/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95906704T 1993-12-23 1994-12-23 Chemilumineszenz-assay mittels energietransfer ATE215696T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17227793A 1993-12-23 1993-12-23
PCT/US1994/014866 WO1995017672A1 (en) 1993-12-23 1994-12-23 Chemiluminescent energy transfer assays

Publications (1)

Publication Number Publication Date
ATE215696T1 true ATE215696T1 (de) 2002-04-15

Family

ID=22627031

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95906704T ATE215696T1 (de) 1993-12-23 1994-12-23 Chemilumineszenz-assay mittels energietransfer

Country Status (8)

Country Link
US (3) US5753436A (de)
EP (1) EP0736174B1 (de)
JP (1) JPH09507571A (de)
AT (1) ATE215696T1 (de)
AU (1) AU1518195A (de)
CA (1) CA2179726A1 (de)
DE (1) DE69430322T2 (de)
WO (1) WO1995017672A1 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0275260B1 (de) 1986-07-24 1993-11-18 Tropix, Inc. Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen
US6022964A (en) 1989-07-17 2000-02-08 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
AU704940B2 (en) * 1995-02-13 1999-05-06 Applied Biosystems, Llc Chemiluminescent energy transfer assays
US5679803A (en) * 1995-10-25 1997-10-21 Tropix, Inc. 1,2 chemiluminescent dioxetanes of improved performance
CA2283434A1 (en) 1997-03-07 1998-09-11 Tropix, Inc. Protease inhibitor assay
WO2000006164A1 (en) * 1998-07-28 2000-02-10 Tropix, Inc. Benzothiazole dioxetanes
US6660529B2 (en) 1998-07-28 2003-12-09 Pe Corporation Heteroaryl substituted benzothiazole dioxetanes
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
CA2370520A1 (en) * 1999-04-16 2000-10-26 Komandoor Elayavalli Achyuthan Viral detection method using viral encoded enzymes and chemiluminescent substrates
US6686171B2 (en) * 1999-05-10 2004-02-03 Tropix, Inc. Competitive chemiluminescent assay for cyclic nucleotide monophosphates
US7160996B1 (en) 1999-06-09 2007-01-09 Bioresearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
WO2000075378A1 (en) * 1999-06-09 2000-12-14 Biosearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
US7368296B2 (en) * 2002-01-17 2008-05-06 Applied Biosystems Solid phases optimized for chemiluminescent detection
US7276343B2 (en) * 2002-06-24 2007-10-02 Fujirebio Inc. Chemiluminescence enhancer
US20040259182A1 (en) * 2003-06-17 2004-12-23 Brooks Edwards Arrays for chemiluminescent assays, methods of making the arrays and methods of detecting chemiluminescent emissions on solid supports
WO2005017191A2 (en) * 2003-06-23 2005-02-24 Nlcpharma, Inc. Enzymatic diagnostic test for sars and other viral diseases
US20050019778A1 (en) * 2003-07-17 2005-01-27 Voyta John C. Sequential generation of multiple chemiluminescent signals on solid supports
US20050026151A1 (en) * 2003-07-17 2005-02-03 Voyta John C. Simultaneous generation of multiple chemiluminescent signals on solid supports
US20050272108A1 (en) * 2004-05-21 2005-12-08 Bhanu Kalra Stabilized two component system for chemiluminescent assay in immunodiagnostics
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
EP1776581B1 (de) 2004-07-19 2015-05-06 ProteinSimple Verfahren zum Nachweis von Proteinen
US7879575B2 (en) * 2005-04-27 2011-02-01 The Trustees Of The University Of Pennsylvania Nanostructures that provide a modified nanoenvironment for the enhancement of luminescence
WO2006116686A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanostructure enhanced luminescent devices
WO2006116687A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanoassays
US20070037174A1 (en) * 2005-08-12 2007-02-15 Rampal Jang B Chemiluminescent generated fluorescent labeling
WO2007035864A2 (en) * 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
WO2007142314A1 (ja) * 2006-06-08 2007-12-13 Fujirebio Inc. 発光増幅剤
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
US20150093757A1 (en) 2013-09-27 2015-04-02 Protein Simple Methods. apparatus and systems for detection of total protein using capillary electrophoresis
WO2017123970A1 (en) 2016-01-13 2017-07-20 ProteinSimple Systems and methods for capillary electrophoresis, isoelectric point, and molecular weight analysis
US11782023B2 (en) 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
CN113841051A (zh) 2019-05-21 2021-12-24 瑞泽恩制药公司 识别和定量宿主细胞蛋白的方法
US11420202B1 (en) 2021-08-31 2022-08-23 ProteinSimple Systems and methods for fractionation and collection of analytes in a sample
US20230075533A1 (en) 2021-09-03 2023-03-09 ProteinSimple Methods and systems for analysis of samples containing particles used for gene delivery

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983779A (en) * 1986-07-17 1991-01-08 The Board Of Governors Of Wayne State University Process for the preparation of vinyl ethers
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US5112960A (en) * 1989-07-17 1992-05-12 Bronstein Irena Y Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes
CA1340590C (en) * 1986-07-24 1999-06-08 John C. Voyta Chemiluminescence enhancement
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
US4956477A (en) * 1987-12-31 1990-09-11 Tropix, Inc. Synthesis of 1,2-dioxetanes
US5220005A (en) * 1986-07-24 1993-06-15 Tropix, Inc. Substituted adamantyl dioxetanes
US4978614A (en) * 1988-10-26 1990-12-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US5639907A (en) 1986-07-24 1997-06-17 Tropix, Inc. Synthesis of 1,2-dioxetanes and intermediates therefor
US5177241A (en) 1987-12-31 1993-01-05 Bronstein Irena Y Synthesis of 1,2-dioxetanes and intermediates therefor
US5538847A (en) * 1989-07-17 1996-07-23 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
US5756770A (en) 1987-12-31 1998-05-26 Tropix, Inc. Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor
US5625077A (en) 1987-12-31 1997-04-29 Tropix, Inc. 1,2-dioxetanes
US5089630A (en) * 1987-12-31 1992-02-18 Bronstein Irena Y Dioxetanes for use in assays
US5679802A (en) 1989-07-17 1997-10-21 Tropix, Inc. 1,2-dioxetanes useful in chemiluminescent immunoassays
US5856522A (en) 1995-05-04 1999-01-05 Tropix, Inc. Method of using synthesis of 1,2-dioxetanes and kits therefore
US5582980A (en) * 1989-07-17 1996-12-10 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
US4952707A (en) * 1988-06-30 1990-08-28 Tropix, Inc. Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
JP2984282B2 (ja) * 1989-06-27 1999-11-29 トロピックス・インコーポレーテッド エンハンサーを用いる化学発光測定法
US5362916A (en) 1989-09-06 1994-11-08 Tropix, Inc. Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts
US5225584A (en) 1989-09-06 1993-07-06 Tropix, Inc. Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor
US5208148A (en) * 1990-12-07 1993-05-04 Molecular Probes, Inc. Lipophilic fluorescent glycosidase substrates
US5336596A (en) * 1991-12-23 1994-08-09 Tropix, Inc. Membrane for chemiluminescent blotting applications
US5679803A (en) * 1995-10-25 1997-10-21 Tropix, Inc. 1,2 chemiluminescent dioxetanes of improved performance

Also Published As

Publication number Publication date
CA2179726A1 (en) 1995-06-29
US5849495A (en) 1998-12-15
US6287767B1 (en) 2001-09-11
EP0736174A1 (de) 1996-10-09
WO1995017672A1 (en) 1995-06-29
JPH09507571A (ja) 1997-07-29
EP0736174A4 (de) 1999-07-28
AU1518195A (en) 1995-07-10
DE69430322D1 (de) 2002-05-08
US5753436A (en) 1998-05-19
EP0736174B1 (de) 2002-04-03
DE69430322T2 (de) 2002-11-14

Similar Documents

Publication Publication Date Title
ATE215696T1 (de) Chemilumineszenz-assay mittels energietransfer
AU3664589A (en) Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
AU582293B2 (en) Heterogeneous specific binding assay employing an aggregatable binding reagent
DE69432709T2 (de) Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen
ATE212722T1 (de) Verbesserte emission bei chemilumineszenzassays
NZ306051A (en) Testing using electrochemiluminescence
NO864709L (no) Gramnegativ bakteriuri-paavisning.
CA2205135A1 (en) Novel 1,2-dioxetane compounds with haloalkoxy groups, methods of preparation and use
NO941781L (no) Ikke-radioaktiv immunanalyse
DE69528375D1 (de) Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung
GR910300015T1 (en) Chemiluminescent 1,2-dioxetane compounds
EP0256932A3 (en) Use of oxidase enzyme systems in chemiluminescent assays
CA2146669A1 (en) Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes
ES8507692A1 (es) Un procedimiento para preparar un trazador para uso en ensayo no isotopico
KR920004839A (ko) 1종 이상의 분석물을 검출하기 위한 동시 검정법
DE69527850T2 (de) Bioluminiscente lyophilisierte bakterien-reagenz zur giftstoff nachweit
AU7958091A (en) Method for detecting and/or assaying hormones and antibodies for use in said method
FI906044A (fi) Buffrad tvaettmedelskomposition oloesliggoerande komposition, testmedel och anvaendningsfoerfarande.
AU2002245206A1 (en) Dendritic chemiluminescent substrates
ES2070129T3 (es) Ensayo de aglutinacion.
IL96115A0 (en) Enzymatic assay and assay kits for detecting and/or measuring the activation of cells
ES2076221T3 (es) Derivados geminales de difenilo y su uso en inmunoensayos.
ES539598A1 (es) Un procedimiento para preparar un trazador cromogeno a base de fluoresceinamina conjugada con l-tiroxina
ES2070715A1 (es) Substratos de aminopeptidasas como sustancias de utilidad diagnosticas.
GB2335038A (en) Double label immunoassay