ATA10372000A - Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen - Google Patents

Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen

Info

Publication number
ATA10372000A
ATA10372000A AT0103700A AT10372000A ATA10372000A AT A10372000 A ATA10372000 A AT A10372000A AT 0103700 A AT0103700 A AT 0103700A AT 10372000 A AT10372000 A AT 10372000A AT A10372000 A ATA10372000 A AT A10372000A
Authority
AT
Austria
Prior art keywords
vector
expression
selection
peptide sequences
randomized peptide
Prior art date
Application number
AT0103700A
Other languages
English (en)
Other versions
AT410217B (de
Original Assignee
Cistem Biotechnologies Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistem Biotechnologies Gmbh filed Critical Cistem Biotechnologies Gmbh
Priority to AT0103700A priority Critical patent/AT410217B/de
Priority to AU2001274097A priority patent/AU2001274097A1/en
Priority to US10/297,969 priority patent/US20040110281A1/en
Priority to PCT/EP2001/006617 priority patent/WO2001096554A1/en
Priority to EP01940566A priority patent/EP1290157A1/de
Publication of ATA10372000A publication Critical patent/ATA10372000A/de
Application granted granted Critical
Publication of AT410217B publication Critical patent/AT410217B/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT0103700A 2000-06-15 2000-06-15 Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen AT410217B (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT0103700A AT410217B (de) 2000-06-15 2000-06-15 Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen
AU2001274097A AU2001274097A1 (en) 2000-06-15 2001-06-12 A vector and a method for expression and selection of random peptide sequences
US10/297,969 US20040110281A1 (en) 2000-06-15 2001-06-12 Vector and a method for expression and selection of random peptide sequences
PCT/EP2001/006617 WO2001096554A1 (en) 2000-06-15 2001-06-12 A vector and a method for expression and selection of random peptide sequences
EP01940566A EP1290157A1 (de) 2000-06-15 2001-06-12 Vektor und methode zur expression und selektion von zufälligen peptidsequenzen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0103700A AT410217B (de) 2000-06-15 2000-06-15 Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen

Publications (2)

Publication Number Publication Date
ATA10372000A true ATA10372000A (de) 2002-07-15
AT410217B AT410217B (de) 2003-03-25

Family

ID=3684449

Family Applications (1)

Application Number Title Priority Date Filing Date
AT0103700A AT410217B (de) 2000-06-15 2000-06-15 Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen

Country Status (5)

Country Link
US (1) US20040110281A1 (de)
EP (1) EP1290157A1 (de)
AT (1) AT410217B (de)
AU (1) AU2001274097A1 (de)
WO (1) WO2001096554A1 (de)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0109428B1 (de) * 1982-05-25 1988-04-06 Brandeis University Klonierungsvektoren die Indikatorgene enthalten, welche durch eine Änderung des Leserasters inaktiviert worden sind
US4503142A (en) * 1982-06-25 1985-03-05 Litton Bionetics, Inc. Open reading frame vectors
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
WO1992022657A1 (en) * 1991-06-13 1992-12-23 Board Of Regents, The University Of Texas System Antigen-enzyme conjugate expression and detection system
US5733731A (en) * 1991-10-16 1998-03-31 Affymax Technologies N.V. Peptide library and screening method
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
JP2879303B2 (ja) * 1993-01-14 1999-04-05 佑 本庶 ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
BR9407211A (pt) * 1993-07-30 1996-09-17 Pfizer Genes codificadores do complexo de desidrogenase de alfa- cetoácidos de cadeia ramificada derivados de streptomyces avermitilis
FR2724183B1 (fr) * 1994-09-02 1997-04-11 Pasteur Institut Vecteurs de criblage et/ou d'expression fonctionnels chez les mycobacteries - utilisation pour l'identification et l'expression de polypeptides exportes

Also Published As

Publication number Publication date
US20040110281A1 (en) 2004-06-10
AU2001274097A1 (en) 2001-12-24
WO2001096554A1 (en) 2001-12-20
AT410217B (de) 2003-03-25
EP1290157A1 (de) 2003-03-12

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Legal Events

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