AR124635A1 - Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas - Google Patents
Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculasInfo
- Publication number
- AR124635A1 AR124635A1 ARP210102859A ARP210102859A AR124635A1 AR 124635 A1 AR124635 A1 AR 124635A1 AR P210102859 A ARP210102859 A AR P210102859A AR P210102859 A ARP210102859 A AR P210102859A AR 124635 A1 AR124635 A1 AR 124635A1
- Authority
- AR
- Argentina
- Prior art keywords
- polyanionic
- impurity
- buffer
- sample
- protein
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 title 1
- 229920002554 vinyl polymer Polymers 0.000 title 1
- 239000012535 impurity Substances 0.000 abstract 12
- 239000003112 inhibitor Substances 0.000 abstract 7
- 239000000872 buffer Substances 0.000 abstract 6
- 238000000034 method Methods 0.000 abstract 6
- 239000012460 protein solution Substances 0.000 abstract 6
- 239000007853 buffer solution Substances 0.000 abstract 5
- 150000001450 anions Chemical class 0.000 abstract 4
- 239000012895 dilution Substances 0.000 abstract 4
- 238000010790 dilution Methods 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 238000002944 PCR assay Methods 0.000 abstract 3
- 239000012530 fluid Substances 0.000 abstract 3
- 230000003139 buffering effect Effects 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 238000011002 quantification Methods 0.000 abstract 2
- 239000011347 resin Substances 0.000 abstract 2
- 229920005989 resin Polymers 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 238000007792 addition Methods 0.000 abstract 1
- 238000005349 anion exchange Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/02—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using precipitation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/137—Concentration of a component of medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/146—Concentration of target or template
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
- Peptides Or Proteins (AREA)
Abstract
Reivindicación 1: Un método para la cuantificación de un inhibidor polianiónico de la PCR en una muestra que comprende: a) Preparar una serie de diluciones de una muestra que comprende al menos cuatro miembros; b) Realizar adiciones a cada miembro de la serie de diluciones de una cantidad constante de un ADN molde distinguible del ADN de la célula hospedadora; c) Realizar un ensayo de PCR en cada miembro de la serie de diluciones y en la cantidad constante del ADN molde en ausencia de cualquier muestra; d) Generar una curva patrón del inhibidor polianiónico; e) Comparar los resultados del ensayo de PCR de la serie de diluciones con los resultados del ensayo de PCR de la cantidad constante del ADN molde en ausencia de cualquier muestra; y f) Identificar la concentración de inhibidor polianiónico de la PCR en la muestra. Reivindicación 9: Un método para retirar un inhibidor polianiónico de la PCR de una solución de tampón que comprende: a) preparar una solución de tampón de una especie tamponante ácida, una especie tamponante básica o una combinación de las mismas; b) poner en contacto la solución de tampón con una resina de modo mixto; y c) separar la solución de tampón de la impureza polianiónica, retirando de este modo la impureza polianiónica de la solución de tampón. Reivindicación 28: Un método para retirar una impureza polianiónica de tampón de una solución de proteínas que comprende: a) ajustar el pH de una solución de proteínas que contiene una impureza aniónica de tampón a un pH inferior al punto isoeléctrico de la proteína en no más de 4 unidades de pH; b) poner en contacto la solución de proteínas con un medio de intercambio aniónico; y c) separar la proteína de la impureza aniónica de tampón. Reivindicación 29: Un método para retirar una impureza polianiónica de tampón de una solución de proteínas que comprende: a) ajustar el pH de una solución de proteínas que contiene una impureza aniónica de tampón a un pH inferior al punto isoeléctrico de la proteína en no más de 4 unidades de pH; b) poner en contacto la solución de proteínas con una resina de modo mixto; y c) separar la proteína de la impureza aniónica de tampón. Reivindicación 33: Un método para la cuantificación de un inhibidor polianiónico de la PCR en una muestra que comprende: (a) poner en contacto una muestra que comprende un inhibidor polianiónico de la PCR con al menos una alícuota de un compuesto policatiónico; (b) añadir un colorante indicador polianiónico en una cantidad suficiente para detectar la forma libre del colorante; y (c) cuantificar el inhibidor polianiónico de la PCR basándose en la cantidad de compuesto policatiónico necesaria para detectar la forma libre del colorante indicador polianiónico. Reivindicación 34: Un método de retirada de una impureza polianiónica en una muestra que comprende: (a) poner en contacto un fluido que comprende una impureza polianiónica con un contraión policatiónico; y (b) separar el fluido de la impureza polianiónica que forma complejo con el contraión policatiónico, retirando de este modo la impureza polianiónica del fluido.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063093120P | 2020-10-16 | 2020-10-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR124635A1 true AR124635A1 (es) | 2023-04-19 |
Family
ID=78536635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP210102859A AR124635A1 (es) | 2020-10-16 | 2021-10-15 | Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20240026432A1 (es) |
| EP (1) | EP4229219A2 (es) |
| JP (1) | JP2023548671A (es) |
| KR (1) | KR20230088396A (es) |
| CN (1) | CN116368239A (es) |
| AR (1) | AR124635A1 (es) |
| AU (1) | AU2021360600A1 (es) |
| CA (1) | CA3193579A1 (es) |
| CL (1) | CL2023001077A1 (es) |
| IL (1) | IL301207A (es) |
| MX (1) | MX2023004408A (es) |
| TW (1) | TW202229858A (es) |
| WO (1) | WO2022081939A2 (es) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6319494B1 (en) | 1990-12-14 | 2001-11-20 | Cell Genesys, Inc. | Chimeric chains for receptor-associated signal transduction pathways |
| IL104570A0 (en) | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| WO2013068107A1 (en) * | 2011-11-07 | 2013-05-16 | Qiagen Gmbh | Lysis method and lysis composition |
| CA3130451A1 (en) * | 2019-02-28 | 2020-09-03 | Day Zero Diagnostics, Inc. | An improved method of preparing clinical samples for nucleic acid amplification |
-
2021
- 2021-10-15 WO PCT/US2021/055117 patent/WO2022081939A2/en active Application Filing
- 2021-10-15 TW TW110138310A patent/TW202229858A/zh unknown
- 2021-10-15 AR ARP210102859A patent/AR124635A1/es unknown
- 2021-10-15 IL IL301207A patent/IL301207A/en unknown
- 2021-10-15 CN CN202180069952.7A patent/CN116368239A/zh active Pending
- 2021-10-15 US US18/029,995 patent/US20240026432A1/en active Pending
- 2021-10-15 CA CA3193579A patent/CA3193579A1/en active Pending
- 2021-10-15 EP EP21805765.1A patent/EP4229219A2/en active Pending
- 2021-10-15 AU AU2021360600A patent/AU2021360600A1/en active Pending
- 2021-10-15 MX MX2023004408A patent/MX2023004408A/es unknown
- 2021-10-15 JP JP2023522437A patent/JP2023548671A/ja active Pending
- 2021-10-15 KR KR1020237015868A patent/KR20230088396A/ko active Search and Examination
-
2023
- 2023-04-14 CL CL2023001077A patent/CL2023001077A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA3193579A1 (en) | 2022-04-21 |
| IL301207A (en) | 2023-05-01 |
| MX2023004408A (es) | 2023-05-04 |
| JP2023548671A (ja) | 2023-11-20 |
| AU2021360600A1 (en) | 2023-03-16 |
| US20240026432A1 (en) | 2024-01-25 |
| TW202229858A (zh) | 2022-08-01 |
| EP4229219A2 (en) | 2023-08-23 |
| CN116368239A (zh) | 2023-06-30 |
| CL2023001077A1 (es) | 2023-12-01 |
| WO2022081939A2 (en) | 2022-04-21 |
| KR20230088396A (ko) | 2023-06-19 |
| WO2022081939A3 (en) | 2022-06-02 |
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