AR124635A1 - Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas - Google Patents

Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas

Info

Publication number
AR124635A1
AR124635A1 ARP210102859A ARP210102859A AR124635A1 AR 124635 A1 AR124635 A1 AR 124635A1 AR P210102859 A ARP210102859 A AR P210102859A AR P210102859 A ARP210102859 A AR P210102859A AR 124635 A1 AR124635 A1 AR 124635A1
Authority
AR
Argentina
Prior art keywords
polyanionic
impurity
buffer
sample
protein
Prior art date
Application number
ARP210102859A
Other languages
English (en)
Inventor
Neil Soice
Scott Kuhns
Andrew Csordas
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AR124635A1 publication Critical patent/AR124635A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/02—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/146—Concentration of target or template
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Un método para la cuantificación de un inhibidor polianiónico de la PCR en una muestra que comprende: a) Preparar una serie de diluciones de una muestra que comprende al menos cuatro miembros; b) Realizar adiciones a cada miembro de la serie de diluciones de una cantidad constante de un ADN molde distinguible del ADN de la célula hospedadora; c) Realizar un ensayo de PCR en cada miembro de la serie de diluciones y en la cantidad constante del ADN molde en ausencia de cualquier muestra; d) Generar una curva patrón del inhibidor polianiónico; e) Comparar los resultados del ensayo de PCR de la serie de diluciones con los resultados del ensayo de PCR de la cantidad constante del ADN molde en ausencia de cualquier muestra; y f) Identificar la concentración de inhibidor polianiónico de la PCR en la muestra. Reivindicación 9: Un método para retirar un inhibidor polianiónico de la PCR de una solución de tampón que comprende: a) preparar una solución de tampón de una especie tamponante ácida, una especie tamponante básica o una combinación de las mismas; b) poner en contacto la solución de tampón con una resina de modo mixto; y c) separar la solución de tampón de la impureza polianiónica, retirando de este modo la impureza polianiónica de la solución de tampón. Reivindicación 28: Un método para retirar una impureza polianiónica de tampón de una solución de proteínas que comprende: a) ajustar el pH de una solución de proteínas que contiene una impureza aniónica de tampón a un pH inferior al punto isoeléctrico de la proteína en no más de 4 unidades de pH; b) poner en contacto la solución de proteínas con un medio de intercambio aniónico; y c) separar la proteína de la impureza aniónica de tampón. Reivindicación 29: Un método para retirar una impureza polianiónica de tampón de una solución de proteínas que comprende: a) ajustar el pH de una solución de proteínas que contiene una impureza aniónica de tampón a un pH inferior al punto isoeléctrico de la proteína en no más de 4 unidades de pH; b) poner en contacto la solución de proteínas con una resina de modo mixto; y c) separar la proteína de la impureza aniónica de tampón. Reivindicación 33: Un método para la cuantificación de un inhibidor polianiónico de la PCR en una muestra que comprende: (a) poner en contacto una muestra que comprende un inhibidor polianiónico de la PCR con al menos una alícuota de un compuesto policatiónico; (b) añadir un colorante indicador polianiónico en una cantidad suficiente para detectar la forma libre del colorante; y (c) cuantificar el inhibidor polianiónico de la PCR basándose en la cantidad de compuesto policatiónico necesaria para detectar la forma libre del colorante indicador polianiónico. Reivindicación 34: Un método de retirada de una impureza polianiónica en una muestra que comprende: (a) poner en contacto un fluido que comprende una impureza polianiónica con un contraión policatiónico; y (b) separar el fluido de la impureza polianiónica que forma complejo con el contraión policatiónico, retirando de este modo la impureza polianiónica del fluido.
ARP210102859A 2020-10-16 2021-10-15 Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas AR124635A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202063093120P 2020-10-16 2020-10-16

Publications (1)

Publication Number Publication Date
AR124635A1 true AR124635A1 (es) 2023-04-19

Family

ID=78536635

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ARP210102859A AR124635A1 (es) 2020-10-16 2021-10-15 Detección de sulfonato de polivinilo y retirada de composiciones de biomoléculas

Country Status (13)

Country Link
US (1) US20240026432A1 (es)
EP (1) EP4229219A2 (es)
JP (1) JP2023548671A (es)
KR (1) KR20230088396A (es)
CN (1) CN116368239A (es)
AR (1) AR124635A1 (es)
AU (1) AU2021360600A1 (es)
CA (1) CA3193579A1 (es)
CL (5) CL2023001077A1 (es)
IL (1) IL301207A (es)
MX (1) MX2023004408A (es)
TW (1) TW202229858A (es)
WO (1) WO2022081939A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL324973A (en) 2023-06-20 2026-01-01 Amgen Inc Chromatography methods and reuse of chromatography media
CN120064520B (zh) * 2025-04-28 2025-08-01 四川科伦博泰生物医药股份有限公司 一种生物样品中抗生素的检测方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319494B1 (en) 1990-12-14 2001-11-20 Cell Genesys, Inc. Chimeric chains for receptor-associated signal transduction pathways
IL104570A0 (en) 1992-03-18 1993-05-13 Yeda Res & Dev Chimeric genes and cells transformed therewith
EP2776577B1 (en) * 2011-11-07 2017-01-11 Qiagen GmbH Lysis method and lysis composition
DE102017222295B4 (de) * 2017-12-08 2019-12-05 Axagarius Gmbh & Co. Kg Kits und Verfahren zur Entfernung von Verunreinigungen aus einer Nukleinsäure enthaltenden Probe
EP3931347A4 (en) * 2019-02-28 2022-11-30 Day Zero Diagnostics, Inc. IMPROVED PROCEDURE FOR PREPARATION OF CLINICAL SAMPLES FOR NUCLEIC ACID AMPLIFICATION

Also Published As

Publication number Publication date
KR20230088396A (ko) 2023-06-19
CL2025003676A1 (es) 2026-01-30
CN116368239A (zh) 2023-06-30
TW202229858A (zh) 2022-08-01
WO2022081939A3 (en) 2022-06-02
CL2025000946A1 (es) 2025-06-23
IL301207A (en) 2023-05-01
AU2021360600A1 (en) 2023-03-16
WO2022081939A2 (en) 2022-04-21
MX2023004408A (es) 2023-05-04
CL2025003680A1 (es) 2026-01-09
CA3193579A1 (en) 2022-04-21
CL2025003677A1 (es) 2026-01-30
JP2023548671A (ja) 2023-11-20
US20240026432A1 (en) 2024-01-25
CL2023001077A1 (es) 2023-12-01
EP4229219A2 (en) 2023-08-23

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