AR120644A1 - Variante de aminotransferasa de aminoácidos de cadena ramificada y procedimiento de producción de leucina usando la misma - Google Patents

Variante de aminotransferasa de aminoácidos de cadena ramificada y procedimiento de producción de leucina usando la misma

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Publication number
AR120644A1
AR120644A1 ARP200103331A ARP200103331A AR120644A1 AR 120644 A1 AR120644 A1 AR 120644A1 AR P200103331 A ARP200103331 A AR P200103331A AR P200103331 A ARP200103331 A AR P200103331A AR 120644 A1 AR120644 A1 AR 120644A1
Authority
AR
Argentina
Prior art keywords
variant
aminotransferase
chain amino
amino acid
same
Prior art date
Application number
ARP200103331A
Other languages
English (en)
Inventor
Hyun Bae
- Kim Ju Eun Jung
Min Ji Baek
Ji Hye Lee
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR120644A1 publication Critical patent/AR120644A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01042—Branched-chain-amino-acid transaminase (2.6.1.42)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La presente se refiere a una variante de aminotransferasa de aminoácidos de cadena ramificada y a un procedimiento de producción de leucina usando la misma. Reivindicación 1: Una variante de aminotransferasa de aminoácidos de cadena ramificada, en la que un aminoácido valina (V) en la posición 156 de un extremo N-terminal de una secuencia de aminoácidos de SEQ ID NÚM.: 1 está sustituido con otro aminoácido. Reivindicación 2: La variante de aminotransferasa de aminoácidos de cadena ramificada de la reivindicación 1, en la que la valina en la posición 156 está sustituida con alanina.
ARP200103331A 2019-12-06 2020-12-01 Variante de aminotransferasa de aminoácidos de cadena ramificada y procedimiento de producción de leucina usando la misma AR120644A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020190161716A KR102143964B1 (ko) 2019-12-06 2019-12-06 신규한 분지쇄 아미노산 아미노트랜스퍼라제 변이체 및 이를 이용한 류신 생산방법

Publications (1)

Publication Number Publication Date
AR120644A1 true AR120644A1 (es) 2022-03-09

Family

ID=72038972

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP200103331A AR120644A1 (es) 2019-12-06 2020-12-01 Variante de aminotransferasa de aminoácidos de cadena ramificada y procedimiento de producción de leucina usando la misma

Country Status (11)

Country Link
US (1) US20220411832A1 (es)
EP (1) EP4047085A4 (es)
JP (1) JP7378621B2 (es)
KR (1) KR102143964B1 (es)
CN (1) CN115038787B (es)
AR (1) AR120644A1 (es)
AU (1) AU2020398425B2 (es)
BR (1) BR112022011021A2 (es)
MX (1) MX2022006827A (es)
WO (1) WO2021112469A1 (es)
ZA (1) ZA202206109B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102143964B1 (ko) * 2019-12-06 2020-08-12 씨제이제일제당 주식회사 신규한 분지쇄 아미노산 아미노트랜스퍼라제 변이체 및 이를 이용한 류신 생산방법
KR102470602B1 (ko) * 2020-12-11 2022-11-25 씨제이제일제당 주식회사 신규한 분지 연쇄 아미노산 아미노트렌스퍼라아제 변이체 및 이를 이용한 이소류신 생산 방법
KR102495918B1 (ko) 2021-01-26 2023-02-06 씨제이제일제당 주식회사 aroG 알돌라아제 (Phospho-2-dehydro-3-deoxyheptonate aldolase) 변이체 및 이를 이용한 분지쇄 아미노산 생산 방법
KR102527096B1 (ko) 2021-02-01 2023-04-28 씨제이제일제당 주식회사 프리페네이트 탈수 효소 (Prephenate dehydratase) 변이체 및 이를 이용한 분지쇄 아미노산 생산 방법
KR102527102B1 (ko) 2021-03-05 2023-04-28 씨제이제일제당 주식회사 이소프로필말레이트 신타제 변이체 및 이를 이용한 l-류신의 생산 방법

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3636722A1 (de) * 1986-10-29 1988-05-05 Hoechst Ag Klonierung und verwendung des transaminase-gens ilve
DE10063314A1 (de) * 2000-12-20 2002-07-04 Degussa Neue für das ilvE-Gen kodierende Nukleotidsequenzen
EP1239041B1 (en) * 2001-02-13 2006-04-19 Ajinomoto Co., Inc. Method for producing L-amino acid using bacteria belonging to the genus Escherichia
RU2243260C2 (ru) 2002-06-25 2004-12-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-лейцина (варианты), штамм escherichia coli 505/pacyc-tyr b - продуцент l-лейцина
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
KR20120098235A (ko) * 2011-02-28 2012-09-05 이화여자대학교 산학협력단 비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법
CN102286505B (zh) * 2011-05-26 2013-04-03 江南大学 用于发酵生产l-缬氨酸的重组dna、菌株及方法
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
KR101796830B1 (ko) 2015-08-25 2017-11-13 씨제이제일제당 (주) Ｌ-류신 생산능을 가지는 미생물 및 이를 이용한 ｌ-류신의 제조방법
ES2907694T3 (es) 2016-08-31 2022-04-26 Cj Cheiljedang Corp Nuevo promotor y uso del mismo
KR102055874B1 (ko) * 2016-12-28 2019-12-13 씨제이제일제당 주식회사 신규한 이소프로필말레이트 신타제 변이체 및 이를 이용한 l-류신의 생산 방법
CN109402034A (zh) 2018-10-26 2019-03-01 江南大学 只产一种支链氨基酸的重组菌及其应用
CN109294966A (zh) 2018-10-26 2019-02-01 江南大学 一种高产l-亮氨酸的谷氨酸棒杆菌重组菌及其构建方法
KR102143964B1 (ko) * 2019-12-06 2020-08-12 씨제이제일제당 주식회사 신규한 분지쇄 아미노산 아미노트랜스퍼라제 변이체 및 이를 이용한 류신 생산방법

Also Published As

Publication number Publication date
ZA202206109B (en) 2023-04-26
EP4047085A4 (en) 2022-12-28
MX2022006827A (es) 2022-07-12
AU2020398425A1 (en) 2022-06-09
WO2021112469A1 (ko) 2021-06-10
CN115038787A (zh) 2022-09-09
AU2020398425B2 (en) 2024-07-11
JP7378621B2 (ja) 2023-11-13
KR102143964B1 (ko) 2020-08-12
BR112022011021A2 (pt) 2022-08-16
EP4047085A1 (en) 2022-08-24
JP2023503077A (ja) 2023-01-26
CN115038787B (zh) 2024-04-12
US20220411832A1 (en) 2022-12-29

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