AR111507A1 - Vacuna recombinante contra helmintos producida en pichia pastoris y procesos de producción y purificación de proteína como vacuna contra helmintos - Google Patents

Vacuna recombinante contra helmintos producida en pichia pastoris y procesos de producción y purificación de proteína como vacuna contra helmintos

Info

Publication number
AR111507A1
AR111507A1 ARP180100137A ARP180100137A AR111507A1 AR 111507 A1 AR111507 A1 AR 111507A1 AR P180100137 A ARP180100137 A AR P180100137A AR P180100137 A ARP180100137 A AR P180100137A AR 111507 A1 AR111507 A1 AR 111507A1
Authority
AR
Argentina
Prior art keywords
helmintos
vaccine against
pichia pastoris
production
protein
Prior art date
Application number
ARP180100137A
Other languages
English (en)
Inventor
Miriam Tendler
Ramos Celso Raul Romero
Andrew J G Simpson
Original Assignee
Miriam Tendler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miriam Tendler filed Critical Miriam Tendler
Publication of AR111507A1 publication Critical patent/AR111507A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C07K14/43559—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from trematodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0003—Invertebrate antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/10—Anthelmintics
    • A61P33/12—Schistosomicides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se relaciona con la producción de proteínas en forma recombinante utilizando un gen sintético para la alta expresión de la proteína en Pichia pastoris. Concretamente se describe la producción de la proteína Sm14 del Schistosoma mansoni en forma recombinante, donde se creó un gen sintético para la alta expresión de esta proteína, y con él se obtuvo la clonación bajo control de dos tipos de promotores de Pichia pastoris: promotor inducible por metanol (AOX1) y promotor constitutivo (GAP). Con estas construcciones se manipularon genéticamente cepas de Pichia pastoris para producir, eficazmente, el antígeno vacunal Sm14. También se perfeccionó el proceso de producción y purificación de esta proteína a partir de células de P. pastoris, pudiendo estar disponible para su producción a escala industrial.
ARP180100137A 2017-01-23 2018-01-22 Vacuna recombinante contra helmintos producida en pichia pastoris y procesos de producción y purificación de proteína como vacuna contra helmintos AR111507A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR102017001309-0A BR102017001309A2 (pt) 2017-01-23 2017-01-23 vacina recombinante para helmintos em pichia pastoris, e, processos de produção e purificação de proteína como vacina para helmintos

Publications (1)

Publication Number Publication Date
AR111507A1 true AR111507A1 (es) 2019-07-24

Family

ID=62907480

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180100137A AR111507A1 (es) 2017-01-23 2018-01-22 Vacuna recombinante contra helmintos producida en pichia pastoris y procesos de producción y purificación de proteína como vacuna contra helmintos

Country Status (9)

Country Link
US (2) US20200010515A1 (es)
EP (1) EP3572514A4 (es)
AR (1) AR111507A1 (es)
AU (1) AU2018210684B2 (es)
BR (1) BR102017001309A2 (es)
CA (1) CA3050981A1 (es)
CO (1) CO2019008755A2 (es)
MX (1) MX2019008681A (es)
WO (1) WO2018132882A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102021012953A2 (pt) 2021-06-29 2023-01-10 Fabp Biotech Desenvolvimento Em Biotecnologia Ltda. Composição de vacina veterinária contra helmintos, método para tratamento e prevenção de infecção causada por helmintos e uso

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0303266B8 (pt) * 2003-01-31 2021-05-25 Fundacao Oswaldo Cruz proteína recombinante sm14, composição imunogênica, e,kit de diagnóstico.
BRPI1005855B1 (pt) * 2010-09-17 2020-04-22 Fundação Oswaldo Cruz processos de produção e purificação de proteina sml 4 recombinante em pichia pastoris

Also Published As

Publication number Publication date
AU2018210684A1 (en) 2019-09-12
BR102017001309A2 (pt) 2018-08-14
EP3572514A4 (en) 2020-12-02
CO2019008755A2 (es) 2020-01-17
EP3572514A1 (en) 2019-11-27
US20240262876A1 (en) 2024-08-08
WO2018132882A1 (pt) 2018-07-26
MX2019008681A (es) 2019-12-09
US20200010515A1 (en) 2020-01-09
CA3050981A1 (en) 2018-07-26
AU2018210684B2 (en) 2024-06-20

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