AR105620A1 - Variantes de quimosina - Google Patents

Variantes de quimosina

Info

Publication number
AR105620A1
AR105620A1 ARP160101858A ARP160101858A AR105620A1 AR 105620 A1 AR105620 A1 AR 105620A1 AR P160101858 A ARP160101858 A AR P160101858A AR P160101858 A ARP160101858 A AR P160101858A AR 105620 A1 AR105620 A1 AR 105620A1
Authority
AR
Argentina
Prior art keywords
isolated
casein
chymosin polypeptide
chymosin
polypeptide variant
Prior art date
Application number
ARP160101858A
Other languages
English (en)
Inventor
Fodor Hansen Enikoe
Lund Martin
Jaeckel Christian
Maarten Van Den Brink Johannes
Jeppesen Iben
Riisberg Lone
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen As filed Critical Chr Hansen As
Publication of AR105620A1 publication Critical patent/AR105620A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/061—Addition of, or treatment with, microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Asimismo se provee un método para producir una variante de polipéptido quimosina y un método para producir un producto alimenticio a base de leche. Reivindicación 1: Una variante de polipéptido de quimosina aislada, caracterizada porque: (a) la variante de polipéptido de quimosina aislada tiene una actividad coagulante específica (IMCU/mg de proteína total) que es al menos un 70% de la actividad coagulante específica del polipéptido de quimosina de camello aislada caracterizado por la SEQ ID Nº 4; y (b) la variante de polipéptido de quimosina aislada escinde la b-caseína con una frecuencia inferior al 50% de la frecuencia de la escisión de b-caseína del polipéptido de quimosina aislada caracterizado por la SEQ ID Nº 4, donde la escisión de b-caseína se determina cuantificando los péptidos de b-caseína obtenidos por incubación de leche descremada con la variante de quimosina o la quimosina de camello, donde la cuantificación se lleva a cabo mediante RP-HPLC acoplada a un espectrómetro de masa ESI-Q-TOF.
ARP160101858A 2015-06-22 2016-06-22 Variantes de quimosina AR105620A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15173099 2015-06-22

Publications (1)

Publication Number Publication Date
AR105620A1 true AR105620A1 (es) 2017-10-25

Family

ID=53496438

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160101858A AR105620A1 (es) 2015-06-22 2016-06-22 Variantes de quimosina

Country Status (13)

Country Link
US (1) US11174473B2 (es)
EP (2) EP3310914B1 (es)
JP (1) JP6856551B2 (es)
KR (1) KR102612929B1 (es)
CN (1) CN107849550B (es)
AR (1) AR105620A1 (es)
AU (1) AU2016282825B2 (es)
BR (1) BR112017027536A2 (es)
CA (1) CA2989488A1 (es)
DK (1) DK3310914T3 (es)
MX (1) MX2017016623A (es)
RU (1) RU2740317C2 (es)
WO (1) WO2016207214A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2855674T5 (pl) 2012-05-25 2023-03-27 Chr. Hansen A/S Warianty chymozyny o ulepszonych właściwościach ścinania mleka
CN111944791A (zh) 2014-02-26 2020-11-17 科·汉森有限公司 具有提高的凝乳特性的凝乳酶变体
EP3310914B1 (en) 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
EP3457860A1 (en) * 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
WO2023001832A1 (en) 2021-07-20 2023-01-26 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
WO2023194285A2 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549390A (en) * 1968-09-09 1970-12-22 Miles Lab Milk-clotting enzyme product and process therefor
FI841288A (fi) 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant-dna-kod foer uttryckning av polypeptid.
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
ES2354775T5 (es) 2000-11-06 2021-12-23 Chr Hansen As Método de producción de quimosina no bovina y uso de la misma
RU2296160C2 (ru) * 2001-02-23 2007-03-27 ДСМ Ай Пи ЭССЕТС Б.В. Новые гены, кодирующие новые протеолитические ферменты
KR101132555B1 (ko) 2002-10-02 2012-04-02 리전츠 오브 더 유니버시티 오브 캘리포니아 변경된 특이성을 갖는 프로테아제의 제조 및 선별 방법
AU2004254352A1 (en) 2003-06-27 2005-01-13 Roberto Crea Look-through mutagenesis
EP1745068A4 (en) 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
WO2010110464A1 (en) * 2009-03-24 2010-09-30 Meito Sangyo Co., Ltd. Improved type milk-clotting protease derived from a microorganism
US10167463B2 (en) 2012-05-03 2019-01-01 Dsm Ip Assets B.V. Modified chymosin polypeptides
PL2855674T5 (pl) * 2012-05-25 2023-03-27 Chr. Hansen A/S Warianty chymozyny o ulepszonych właściwościach ścinania mleka
KR20150062717A (ko) * 2013-11-29 2015-06-08 롯데푸드 주식회사 발효 크림치즈 제조방법
CN111944791A (zh) * 2014-02-26 2020-11-17 科·汉森有限公司 具有提高的凝乳特性的凝乳酶变体
KR20170115614A (ko) 2015-02-10 2017-10-17 시에이치알. 한센 에이/에스 개선된 응유 특성을 갖는 키모신 배합물
EP3310914B1 (en) 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
EP3344049A1 (en) 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3457860A1 (en) * 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN109688825A (zh) 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体

Also Published As

Publication number Publication date
CN107849550B (zh) 2021-09-03
KR20180021098A (ko) 2018-02-28
CA2989488A1 (en) 2016-12-29
BR112017027536A2 (pt) 2018-09-11
RU2017144572A (ru) 2019-07-22
CN107849550A (zh) 2018-03-27
JP2018525974A (ja) 2018-09-13
WO2016207214A1 (en) 2016-12-29
AU2016282825B2 (en) 2022-11-17
DK3310914T3 (da) 2022-06-20
RU2740317C2 (ru) 2021-01-13
AU2016282825A1 (en) 2018-01-04
KR102612929B1 (ko) 2023-12-12
MX2017016623A (es) 2018-05-15
EP3310914B1 (en) 2022-04-06
US20180187179A1 (en) 2018-07-05
EP4079849A1 (en) 2022-10-26
EP3310914A1 (en) 2018-04-25
US11174473B2 (en) 2021-11-16
RU2017144572A3 (es) 2020-01-27
JP6856551B2 (ja) 2021-04-07

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