AR096314A1 - Métodos para la producción y/o purificación de hormonas - Google Patents
Métodos para la producción y/o purificación de hormonasInfo
- Publication number
- AR096314A1 AR096314A1 ARP140101955A ARP140101955A AR096314A1 AR 096314 A1 AR096314 A1 AR 096314A1 AR P140101955 A ARP140101955 A AR P140101955A AR P140101955 A ARP140101955 A AR P140101955A AR 096314 A1 AR096314 A1 AR 096314A1
- Authority
- AR
- Argentina
- Prior art keywords
- hormones
- purification
- hormone
- production
- recombinant
- Prior art date
Links
- 229940088597 hormone Drugs 0.000 title abstract 6
- 239000005556 hormone Substances 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 238000000746 purification Methods 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 abstract 2
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 abstract 2
- 230000003115 biocidal effect Effects 0.000 abstract 2
- 229940028334 follicle stimulating hormone Drugs 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 230000004071 biological effect Effects 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 238000012761 co-transfection Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 238000004811 liquid chromatography Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000002684 recombinant hormone Substances 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
- 238000001542 size-exclusion chromatography Methods 0.000 abstract 1
- 238000000825 ultraviolet detection Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 238000001262 western blot Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Reproductive Health (AREA)
- Endocrinology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Genetics & Genomics (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Gynecology & Obstetrics (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Pregnancy & Childbirth (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere a métodos para producción y/o purificación de hormonas, particularmente la hormona folículo estimulante (FSH), de origen humano o animal, utilizando una plataforma de células humanas, así como los productos así obtenidos y sus usos. Reivindicación 3: Método para la producción de hormonas que comprende las siguientes etapas: 1) extracción de ARN total de una muestra de tejido; 2) síntesis de ADNc mediante transcripción inversa a partir del ARN obtenido en la etapa 1; 3) amplificación de los fragmentos de ADNc correspondientes a las cadenas (a y b) completas; 4) clonado de cada uno de los fragmentos amplificados (a y b) en un vector de expresión para células de mamífero; 5) co-transfección de ambos vectores que contienen las cadenas a y b, junto con un vector que contiene un gen de resistencia a antibiótico en una línea celular de células de riñón embrionario humano (HEK) o células derivadas; 6) selección de clones de células recombinantes mediante su resistencia al antibiótico; 7) detección opcional de la expresión de la proteína recombinante mediante Western Blotting y ensayo de la actividad biológica in vitro e in vivo; 8) purificación opcional de la hormona obtenida. Reivindicación 10: Método para la purificación de hormonas que comprende someter el sobrenadante de cultivo que contiene la hormona recombinante a las siguientes etapas: a) cromatografía de afinidad por colorante, b) cromatografía de intercambio iónico, c) cromatografía de exclusión por tamaño. Reivindicación 22: Método para análisis de hormonas directamente en un medio de cultivo celular que comprende una etapa de cromatografía líquida con detección de UV (254 nm) y columna C₁₈, en donde las corridas son llevadas a cabo con una fase móvil de metanol, etanol y agua, caudal de 1 ml/min, temperatura de horno 40ºC y volumen de inyección de muestra de 20 ml.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/BR2013/000165 WO2014183175A1 (en) | 2013-05-16 | 2013-05-16 | Methods for production and/or purification of hormones |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR096314A1 true AR096314A1 (es) | 2015-12-23 |
Family
ID=51897526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP140101955A AR096314A1 (es) | 2013-05-16 | 2014-05-15 | Métodos para la producción y/o purificación de hormonas |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR096314A1 (es) |
| BR (1) | BR112015028737B1 (es) |
| WO (1) | WO2014183175A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR102015032660B1 (pt) * | 2015-12-28 | 2019-05-28 | Ouro Fino Saúde Animal Participações S.A. | PROCESSO DE PRODUÇÃO DE UMA GONADOTROFINA CORIÔNICA EQUINA RECOMBINANTE (reCG): COMPOSIÇÃO VETERINÁRIA E USO |
| CN108531508B (zh) * | 2018-03-20 | 2021-07-16 | 西北民族大学 | 一种绵羊促卵泡激素基因重组表达制备方法 |
| CL2018001347A1 (es) | 2018-05-18 | 2019-03-15 | Centro De Biotecnologia Y Biomedicina Spa 94% | Hormona folículo estimulante bovina recombinante, composición que la comprende y método para inducir superovulación y sincronización del celo en bovinos utilizando dicha hormona |
| BR102019004147A2 (pt) * | 2019-02-28 | 2020-10-06 | Ouro Fino Saude Animal Participacoes S.A. | Gonadotrofina coriônica equina recombinante (recg) biologicamente ativa e processo para obtenção da mesma, composição veterinária e uso |
| CN109970871A (zh) * | 2019-04-28 | 2019-07-05 | 广州威生医药科技有限公司 | 长效重组fsh融合蛋白、其制备方法及其在母猪定时输精中的应用 |
| CN114591414A (zh) * | 2022-03-24 | 2022-06-07 | 江西浩然生物制药有限公司 | 一种人绒毛膜促性腺激素的制备方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4840896A (en) * | 1983-11-02 | 1989-06-20 | Integrated Genetics, Inc. | Heteropolymeric protein |
| US6737515B2 (en) * | 1993-11-19 | 2004-05-18 | Washington University | Follicle stimulating hormone-glycosylation analogs |
| UA90664C2 (ru) * | 2003-12-22 | 2010-05-25 | Ейрес Трейдінг С.А. | Способ очистки fsh |
| IL162258A (en) * | 2004-05-31 | 2010-11-30 | Merck Serono Sa | Method for improving homogeneity and/or secretion of a recombinant protein |
| CA2625978A1 (en) * | 2005-12-09 | 2007-06-14 | Ares Trading S.A. | Method for purifying fsh or a fsh mutant |
| JP2009273427A (ja) * | 2008-05-16 | 2009-11-26 | Jcr Pharmaceuticals Co Ltd | 組換え体ヒトｆｓｈの製造方法 |
-
2013
- 2013-05-16 WO PCT/BR2013/000165 patent/WO2014183175A1/en active Application Filing
- 2013-05-16 BR BR112015028737-9A patent/BR112015028737B1/pt active IP Right Grant
-
2014
- 2014-05-15 AR ARP140101955A patent/AR096314A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014183175A1 (en) | 2014-11-20 |
| BR112015028737B1 (pt) | 2022-05-10 |
| BR112015028737A2 (pt) | 2018-01-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |