AR085619A2 - Un kit de reactivo para detectar un analito en una muestra - Google Patents

Un kit de reactivo para detectar un analito en una muestra

Info

Publication number
AR085619A2
AR085619A2 ARP120100712A AR085619A2 AR 085619 A2 AR085619 A2 AR 085619A2 AR P120100712 A ARP120100712 A AR P120100712A AR 085619 A2 AR085619 A2 AR 085619A2
Authority
AR
Argentina
Prior art keywords
nucleic acid
reaction
click
alkyne
functional group
Prior art date
Application number
Other languages
English (en)
Original Assignee
Basf Ag
Univ Muenchen L Maximilians
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Univ Muenchen L Maximilians filed Critical Basf Ag
Publication of AR085619A2 publication Critical patent/AR085619A2/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Un kit de reactivo para detectar un analito en una muestra que comprende: (a) un compuesto funcionalizado que comprende: (i) al menos un grupo funcional Click seleccionado de los grupos alquino y azida que es un primer compañero de reacción para una reacción Click, dicha reacción Click es una reacción de cicloadición (3 + 2) entre grupos azida y alquino que resulta en la formación de un anillo 1,2,3-triazol, y (ii) un bloque de construcción de ácido nucleico o análogo de ácido nucleico, en donde el grupo funcional se encuentra unido a una nucleobase, (b) un segundo compañero de reacción para dicha reacción Click que comprende el grupo azida o alquino complementario, en donde el segundo compañero de reacción además comprende un grupo marcador o un grupo precursor de un grupo marcador, y (c) opcionalmente un reactivo que forma marcadores, capaz de convertir grupos precursores marcadores a grupos marcadores.Reivindicación 3: Uso del kit de reactivo de acuerdo con la reivindicación 1, caracterizado porque el compuesto funcionalizado es un compuesto de fórmula (1): C-S-N     (1) en donde C es un grupo funcional Click seleccionado del los grupos alquino y azida que es un primer compañero de reacción para una reacción Click, dicha reacción Click es una reacción de cicloadición (3 + 2) entre grupos azida y alquino que resulta en la formación de un anillo 1,2,3-triazol; S es un espaciador; y, N es un bloque de construcción de ácido nucleico o análogo de ácido nucleico, en donde el grupo funcional Click se encuentra unido a una nucleobase, en donde el compuesto de fórmula (1) forma un producto de asociación con el analito a ser detectado. Reivindicación 15: Un ácido nucleico o una molécula de un análogo de ácido nucleico caracterizado porque tiene incorporada en la misma al menos un compuesto de la fórmula (1) de acuerdo a cualquiera de las reivindicaciones 3 a 14, en donde el grupo funcional es un grupo alquino unido a las posiciones 5 y 6, preferentemente a la posición 5, de una nucleobase de pirimidina, o a las posiciones 7 y 8, preferentemente a la posición 7 de una nucleobase de purina, y en donde la molécula de ácido nucleico o de análogo de ácido nucleico es capaz de hibridizarse con un ácido nucleico complementario. Reivindicación 17: Un método para sintetizar un ácido nucleico o una molécula de un análogo de ácido nucleico que es capaz de de hibridizarse con un ácido nucleico complementario, caracterizado porque comprende incorporar un nucleótido o un bloque de construcción de un análogo de nucleótido que comprende un compuesto de fórmula (1) como se define en cualquiera de las reivindicaciones 4 a 14, en donde el grupo funcional es un grupo alquino unido a las posiciones 5 y 6, preferentemente a la posición 5, de una nucleobase de pirimidina, o a las posiciones 7 y 8, preferentemente a la posición 7 de una nucleobase de purina, dentro de una molécula de ácido nucleico o análogo de ácido nucleico. Reivindicaron 22: Uso del kit de reactivo de la reivindicación 1, caracterizado porque es para aplicaciones de agricultura. Reivindicación 27: Uso de cualquier a de las reivindicaciones 22 a 26, caracterizado porque es para detectar y/o diferenciar organismos o cepas genéticamente modificadas, por ejemplo, organismos o cepas de hongos, insectos o plantas.
ARP120100712 2005-05-02 2012-03-05 Un kit de reactivo para detectar un analito en una muestra AR085619A2 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US67678505P 2005-05-02 2005-05-02
EP05009618 2005-05-02
US75010005P 2005-12-14 2005-12-14
EP05027370 2005-12-14

Publications (1)

Publication Number Publication Date
AR085619A2 true AR085619A2 (es) 2013-10-16

Family

ID=36922226

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120100712 AR085619A2 (es) 2005-05-02 2012-03-05 Un kit de reactivo para detectar un analito en una muestra

Country Status (4)

Country Link
US (4) US20120107943A1 (es)
KR (1) KR101335218B1 (es)
AR (1) AR085619A2 (es)
HR (1) HRP20100659T1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3591069A3 (en) 2005-10-27 2020-03-04 The President and Fellows of Harvard College Methods and compositions for labeling nucleic acids
CN103454268B (zh) * 2013-04-19 2016-02-24 南京工业大学 一种基于点击反应的还原糖定量检测方法
WO2019067635A1 (en) 2017-09-27 2019-04-04 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING
CN112147335A (zh) * 2019-06-28 2020-12-29 深圳市帝迈生物技术有限公司 一种基于点击化学的标记配体组合物、试剂盒及系统

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EP3591069A3 (en) 2005-10-27 2020-03-04 The President and Fellows of Harvard College Methods and compositions for labeling nucleic acids
JP5646172B2 (ja) 2006-10-31 2014-12-24 ベースクリック ゲーエムベーハー リポーター分子の製造のためのクリックケミストリー

Also Published As

Publication number Publication date
US20120190838A1 (en) 2012-07-26
US20120157327A1 (en) 2012-06-21
US20120172582A1 (en) 2012-07-05
HRP20100659T1 (hr) 2011-01-31
KR101335218B1 (ko) 2013-12-12
US9005892B2 (en) 2015-04-14
KR20080011314A (ko) 2008-02-01
US20120107943A1 (en) 2012-05-03

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