AR068224A1 - Composicion para separar espermatozoides de una muestra de semen y metodos para separar espermatozoides a partir de una muestra de semen de un animal no humano - Google Patents

Composicion para separar espermatozoides de una muestra de semen y metodos para separar espermatozoides a partir de una muestra de semen de un animal no humano

Info

Publication number
AR068224A1
AR068224A1 ARP080103878A ARP080103878A AR068224A1 AR 068224 A1 AR068224 A1 AR 068224A1 AR P080103878 A ARP080103878 A AR P080103878A AR P080103878 A ARP080103878 A AR P080103878A AR 068224 A1 AR068224 A1 AR 068224A1
Authority
AR
Argentina
Prior art keywords
sperm
semen sample
composition
semen
spermatozoids
Prior art date
Application number
ARP080103878A
Other languages
English (en)
Original Assignee
Morrell Jane
Rodriguez Martinez Heriberto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morrell Jane, Rodriguez Martinez Heriberto filed Critical Morrell Jane
Publication of AR068224A1 publication Critical patent/AR068224A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Composiciones y métodos utiles para procesar semen animal para usar en la reproduccion asistida. Una composicion para separar los espermatozoides de una muestra de semen que comprende al menos una sal de un metal alcalino y/o un metal alcalino térreo, EDTA, un amortiguador zwitterrionico, partículas de sílice recubiertas con silano y agua, donde dicha composicion tiene un pH de 7,0-7,35 y una osmolaridad de 300-345 mOsm. Además, un método para preparar espermatozoides a partir de una muestra de semen de un animal no humano, que comprende el paso de separar los espermatozoides de los otros constituyentes del semen por centrifugacion a través de una sola capa de una formulacion coloidal. Preferiblemente, la formulacion coloidal es la composicion mencionada. Un método para separar una subpoblacion de esperma de interés de una muestra de semen de un animal no humano, que comprende los pasos de proveer un gradiente de densidad que comprende al menos dos capas de la composicion, donde cada capa tiene una densidad diferente; separar las subpoblaciones de esperma en la muestra de semen por centrifugacion a través del gradiente de densidad; y seleccionar la subpoblacion de esperma de interés. Finalmente se proveen espermatozoides preparados con los métodos de la presente y con el uso de dichos espermatozoides en inseminacion artificial, fertilizacion in vitro o inyeccion intracitoplásmica de esperma.
ARP080103878A 2007-09-07 2008-09-05 Composicion para separar espermatozoides de una muestra de semen y metodos para separar espermatozoides a partir de una muestra de semen de un animal no humano AR068224A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0701999 2007-09-07

Publications (1)

Publication Number Publication Date
AR068224A1 true AR068224A1 (es) 2009-11-11

Family

ID=40429131

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080103878A AR068224A1 (es) 2007-09-07 2008-09-05 Composicion para separar espermatozoides de una muestra de semen y metodos para separar espermatozoides a partir de una muestra de semen de un animal no humano

Country Status (9)

Country Link
US (1) US8939887B2 (es)
EP (1) EP2198015B1 (es)
AR (1) AR068224A1 (es)
BR (1) BRPI0816375B1 (es)
CA (1) CA2698988C (es)
ES (1) ES2656799T3 (es)
SA (1) SA08290560B1 (es)
SE (1) SE535788C2 (es)
WO (1) WO2009031970A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102620974A (zh) * 2012-04-06 2012-08-01 重庆大学 一种从天然水体悬浮物中富集纯化藻类细胞的方法
CN105287045B (zh) * 2015-10-15 2017-01-04 燕海峰 一种可用于种鸡场人工输精的精液稀释方法
CN111607559A (zh) * 2020-05-29 2020-09-01 太东(镇江)生物科技有限公司 一种精子分离液及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840502A (en) * 1994-08-31 1998-11-24 Activated Cell Therapy, Inc. Methods for enriching specific cell-types by density gradient centrifugation
JP4421681B2 (ja) * 1995-10-19 2010-02-24 バイオ‐オリジン エルエルシー 生殖細胞および胚の生存および機能を改善する方法および組成物
US7674576B2 (en) * 2001-01-12 2010-03-09 Abs Corporation Semen extender composition and methods for manufacturing and using
AU2002318154B2 (en) * 2001-05-21 2007-10-04 Safety Art, Llc Method and apparatus for reducing pathogens in a biological sample
GB0905677D0 (en) * 2009-04-01 2009-05-20 Yes Syzygy Ltd Intimate lubrication kit

Also Published As

Publication number Publication date
EP2198015B1 (en) 2017-10-25
BRPI0816375B1 (pt) 2019-07-16
SE0950143L (sv) 2009-03-11
WO2009031970A1 (en) 2009-03-12
SA08290560B1 (ar) 2012-02-22
CA2698988C (en) 2017-01-10
US20110004051A1 (en) 2011-01-06
SE535788C2 (sv) 2012-12-18
BRPI0816375A2 (pt) 2015-02-24
US8939887B2 (en) 2015-01-27
ES2656799T3 (es) 2018-02-28
CA2698988A1 (en) 2009-03-12
EP2198015A4 (en) 2011-01-19
EP2198015A1 (en) 2010-06-23

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