AR065746A1 - Constructos de adn de repeticion en tandem, productores de proteinas que atacan virus, hongos y bacterias patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales para que los mencionados agentes patogenos hagan su replicacion en las plantas - Google Patents
Constructos de adn de repeticion en tandem, productores de proteinas que atacan virus, hongos y bacterias patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales para que los mencionados agentes patogenos hagan su replicacion en las plantasInfo
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- AR065746A1 AR065746A1 ARP080102218A ARP080102218A AR065746A1 AR 065746 A1 AR065746 A1 AR 065746A1 AR P080102218 A ARP080102218 A AR P080102218A AR P080102218 A ARP080102218 A AR P080102218A AR 065746 A1 AR065746 A1 AR 065746A1
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- trs
- zfp
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
- C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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Abstract
Métodos y composiciones referidas al tratamiento, al control o la inhibicion de Geminivirus, mediante la reduccion o la inhibicion del crecimiento de los Geminivirus (GV) que emplean un compuesto que tiene la estructura siguiente (1), o una sal farmacéuticamente aceptables de ese compuesto, caracterizado porque a R1, R2, R3 y R4 se seleccionan del grupo consistente en un grupo carboxilo, un grupo metilo, un grupo etilo, un grupo propilo, un grupo isopropilo, un grupo butilo, un grupo isobutilo, un grupo butilo secundario, un grupo butilo terciario, un grupo pentilo, un grupo isopentilo, un grupo neopentilo, fluor, cloro, bromo, yodo y hidrogeno. Brinda varias clases de compuestos antivirosicos que se puede utilizar o desintegrar geminivirus, tales como los geminivirus africanos del mosaico de la casava (ACMV) y los geminivirus del Este de África del mosaico de la casava (EAMGV), a través del ataque del dominio de dedos de zinc [Zn2+]-[CCHC] o de variaciones de él, el tal dominio estando presente en la nucelocápside u otras ZFP virosicas. A los agentes antivirosicos se los diseno para usárselos en plantas infectadas por virus patogenos. Estos agentes antivirosicos comprenden el Ácido Picolínico (PA) y derivados de él. Los agentes pueden ligar de manera coordinada el Zn2+ a la proteína del virus y expulsar el Zn2+ de la ZFP o se pueden ligar al Zn2+ y formar un complejo ternario con la ZFP [PA-Zn2+-ZFP] virosica. Además, también se desarrollo un sistema disenado para detectar e identificar derivados antivirosicos de ácido picolínico contra los GV, basado sobre la capacidad de estos derivados para reaccionar e interrumpir los motivos de CCHC (o una variacion de ellos) en la ZFP de los GV. Los agentes y métodos antivirosicos que se describe en el presente que comprenden el empleo de ácido picolínico, ácido fusárico y derivados de estos agregados en forma exogena, también se pueden emplear para prevenir, controlar o eliminar cualquier otro virus de plantas que contenga ZFP esenciales en el proteoma o que utilice las ZFP de la célula hospedante para la activacion transcripcional. Además, se da a conocer una tecnología de Secuencia Repetida de Tandem (TRS), denotada como método de Constructo de TRS, para la produccion de plantas singénicas con resistencia aumentada a los virus. Esta tecnología incorpora, en el genoma de la planta, secuencias de ADN estables repetidas en tandem, que codifican enzimas que pueden producir PA o derivados de él. La produccion aumentada de PA endogeno por la Carboxilasa de Ácido Picolínico, inducida por un promotor de proteína virosica por TRS que está presente en el Constructo de TRS, puede inactivar e interrumpir la ZFP virosicas necesarias para la replicacion de los virus. Las plantas singénicas en las que se introdujo el Constructo de TRS para conferir resistencia a los virus son genéticamente estables y fértiles. Las plantas singénicas con TRS producen flores y semillas que se puede volver a plantar para producir plantas singénicas con TRS que son resistentes a los virus. Por anadidura, las plantas singénicas con TRS que obedecen al presente están sujetas a las presiones evolutivas del ambiente, tal como si fueran la contraparte de tipo silvestre. Más aun; dado que no contienen agentes agregados en forma exogena o endogena, son comestibles sin que haya que hacerles más modificaciones.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/051,060 US20090241221A1 (en) | 2008-03-19 | 2008-03-19 | Tandem reapeat dna constructs producing proteins that attack plant pathogenic viruses, fungi, and bacteria by disrupting transcription factors essential for replication thereof in plants |
Publications (1)
Publication Number | Publication Date |
---|---|
AR065746A1 true AR065746A1 (es) | 2009-07-01 |
Family
ID=40974305
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP080102218A AR065746A1 (es) | 2008-03-19 | 2008-05-27 | Constructos de adn de repeticion en tandem, productores de proteinas que atacan virus, hongos y bacterias patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales para que los mencionados agentes patogenos hagan su replicacion en las plantas |
Country Status (2)
Country | Link |
---|---|
US (1) | US20090241221A1 (es) |
AR (1) | AR065746A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113077840B (zh) * | 2021-04-21 | 2023-01-31 | 四川大学 | 基于药效团与alpha-碳特征的金属酶活性位点对比方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5243041A (en) * | 1991-08-22 | 1993-09-07 | Fernandez Pol Jose A | DNA vector with isolated CDNA gene encoding metallopanstimulin |
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2008
- 2008-03-19 US US12/051,060 patent/US20090241221A1/en not_active Abandoned
- 2008-05-27 AR ARP080102218A patent/AR065746A1/es unknown
Also Published As
Publication number | Publication date |
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US20090241221A1 (en) | 2009-09-24 |
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