AR045420A1 - MODULATION OF CYTOKININ ACTIVITY IN PLANTS - Google Patents

MODULATION OF CYTOKININ ACTIVITY IN PLANTS

Info

Publication number
AR045420A1
AR045420A1 ARP040101154A ARP040101154A AR045420A1 AR 045420 A1 AR045420 A1 AR 045420A1 AR P040101154 A ARP040101154 A AR P040101154A AR P040101154 A ARP040101154 A AR P040101154A AR 045420 A1 AR045420 A1 AR 045420A1
Authority
AR
Argentina
Prior art keywords
plant
seeds
expression
heterologous nucleotide
plants
Prior art date
Application number
ARP040101154A
Other languages
Spanish (es)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR045420A1 publication Critical patent/AR045420A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se relaciona en general con el campo de la biología molecular vegetal. Más específicamente, se relaciona con métodos y reactivos para la expresión regulada en el tiempo y/o en el espacio de los genes que afectan los niveles metabólicamente eficaces de las citoquininas en plantas, en particular en semillas y en el tejido reproductor femenino relacionado. Ser relaciona además con plantas transgénicas que presentan mayores niveles de expresión de citoquininas, donde dichas plantas transgénicas exhiben características que son de utilidad, tal como mayor tamaño de las semillas, menor cantidad de abortos de los granos más jóvenes, mayor establecimiento de semillas bajo condiciones ambientales desfavorables o estabilidad del rendimiento. Se provee composiciones y métodos para regular la expresión de secuencias de nucleótidos heterólogos en una planta. Las composiciones comprenden nuevas secuencias de nucleótidos correspondientes a promotores con preferencia por semillas, conocidos como eep1 y eep2. Se provee asimismo un método para expresar una secuencia de nucleótidos heteróloga en una planta usando las secuencias promotoras que se describen en la presente. El método comprende transformar una célula vegetal para que contenga una secuencia de nucleótidos heteróloga ligada operativamente a uno de los promotores del presente y regenerar una planta transformada de forma estable a partir de la célula vegetal transformada.It is related in general to the field of plant molecular biology. More specifically, it relates to methods and reagents for the expression regulated in time and / or in the space of genes that affect the metabolically effective levels of cytokinins in plants, particularly in seeds and in the related female reproductive tissue. Being also relates to transgenic plants that have higher levels of cytokinin expression, where these transgenic plants exhibit characteristics that are useful, such as larger seed sizes, less abortion of younger grains, greater establishment of seeds under conditions Unfavorable environmental or performance stability. Compositions and methods are provided to regulate the expression of heterologous nucleotide sequences in a plant. The compositions comprise new nucleotide sequences corresponding to promoters with preference for seeds, known as eep1 and eep2. A method is also provided to express a heterologous nucleotide sequence in a plant using the promoter sequences described herein. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operatively linked to one of the promoters of the present and regenerating a plant stably transformed from the transformed plant cell.

ARP040101154A 2003-04-04 2004-04-06 MODULATION OF CYTOKININ ACTIVITY IN PLANTS AR045420A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US46071803P 2003-04-04 2003-04-04

Publications (1)

Publication Number Publication Date
AR045420A1 true AR045420A1 (en) 2005-10-26

Family

ID=33159802

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040101154A AR045420A1 (en) 2003-04-04 2004-04-06 MODULATION OF CYTOKININ ACTIVITY IN PLANTS

Country Status (9)

Country Link
EP (1) EP1613754A2 (en)
CN (3) CN101220358A (en)
AR (1) AR045420A1 (en)
AU (1) AU2004227360B2 (en)
BR (1) BRPI0409178A (en)
CA (1) CA2521497C (en)
NZ (1) NZ543070A (en)
WO (1) WO2004090143A2 (en)
ZA (1) ZA200508006B (en)

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Also Published As

Publication number Publication date
ZA200508006B (en) 2007-01-31
EP1613754A2 (en) 2006-01-11
CA2521497A1 (en) 2004-10-21
CA2521497C (en) 2012-11-27
WO2004090143A2 (en) 2004-10-21
CN101220357A (en) 2008-07-16
AU2004227360A1 (en) 2004-10-21
AU2004227360B2 (en) 2009-05-28
WO2004090143A3 (en) 2005-05-12
CN1798843A (en) 2006-07-05
BRPI0409178A (en) 2006-05-02
CN101220358A (en) 2008-07-16
NZ543070A (en) 2008-10-31

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