AR016185A1 - METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS - Google Patents

METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS

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Publication number
AR016185A1
AR016185A1 ARP990101003A AR016185A1 AR 016185 A1 AR016185 A1 AR 016185A1 AR P990101003 A ARP990101003 A AR P990101003A AR 016185 A1 AR016185 A1 AR 016185A1
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AR
Argentina
Prior art keywords
plants
seeds
rna sequence
plant cells
dna
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Application number
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Spanish (es)
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Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR016185A1 publication Critical patent/AR016185A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Agronomy & Crop Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Comprende un método para producir plantas con esterilidad masculina, caracterizada porque comprende los pasos de: insertar en el genoma de una célulavegetal: una primera molécula de ADN que comprende, ligado operativamente en la orientacion 5 a 3; unprimer promotor que funciona en células vegetalescausando la produccion de una primera secuencia de ARN; una primera secuencia de ADN que codifica una proteína que produce tolerancia al glifosato; una primeraregion no traducida 3 que funciona en células vegetales causando la polialdenilacion del extremo 3 de la primera secuencia de ARN; y una segunda molécula deADN que comprende, ligado operativamente en la orientacion 5 a 3; un segundo promotor que funciona en células vegetales causando la produccion de una segundasecuencia de ARN en un tejido reproductor masculino; una segunda secuencia de ADN que codifica una segunda secuencia de ARN que es complementaria de la primerasecuencia de ARN; una segunda region no traducida 3 que funciona encélulas vegetales causando la poliadenilacion del extremo 3 de la segunda secuencia deARN; obtener células vegetales transformadas que contienen la primera y segunda molécula de ADN; regenerrar plantas transformadas a partir de las célulasvegetales transformadas; y exponer las plantas transformadas a glifosato. Las plantas que contienen la primera y la segunda molécula de ADN se vuelvenmasculinos estériles por exposicion a glifosato. Los métodos y las composiciones de la invencion resultan ventajosos para su uso en la generacion de semillashíbridas, para restringir el cruzamiento con especies relacionadas y para prolongar la vida de las flores.It comprises a method for producing plants with male sterility, characterized in that it comprises the steps of: inserting into the genome of a vegetative cell: a first DNA molecule that comprises, operatively linked in orientation 5 to 3; a first promoter that works in plant cells causing the production of a first RNA sequence; a first DNA sequence that encodes a protein that produces glyphosate tolerance; a first untranslated region 3 that functions in plant cells causing polyaldenylation of end 3 of the first RNA sequence; and a second DNA molecule comprising, operably linked in the 5 to 3 orientation; a second promoter that works in plant cells causing the production of a second RNA sequence in a male reproductive tissue; a second DNA sequence encoding a second RNA sequence that is complementary to the first RNA sequence; a second untranslated region 3 that operates plant cells causing polyadenylation of end 3 of the second RNA sequence; obtain transformed plant cells that contain the first and second DNA molecules; regenerate transformed plants from transformed vegetable cells; and expose the transformed plants to glyphosate. Plants that contain the first and second DNA molecules become sterile by exposure to glyphosate. The methods and compositions of the invention are advantageous for use in the generation of hybrid seeds, to restrict crossbreeding with related species and to prolong the life of the flowers.

ARP990101003 1998-03-09 1999-03-09 METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS AR016185A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7727798P 1998-03-09 1998-03-09

Publications (1)

Publication Number Publication Date
AR016185A1 true AR016185A1 (en) 2001-06-20

Family

ID=22137136

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990101003 AR016185A1 (en) 1998-03-09 1999-03-09 METHOD FOR PRODUCING PLANTS WITH MALE STERILITY; SUCH PLANTS; VEGETABLE CELLS THAT CONTAIN IN THEIR GENOMA A FIRST DNA MOLECULA, METODOPARA TO PRODUCE HYBRID SEEDS, SUCH SEEDS AND THE PLANTS ACHIEVED OF THESE, AND TRANSGENIC PLANTS AND SEEDS WITH THE CURRENT VEGETABLE CELLS

Country Status (6)

Country Link
EP (1) EP1062354A2 (en)
CN (1) CN1300324A (en)
AR (1) AR016185A1 (en)
AU (1) AU2992999A (en)
BR (1) BR9908631A (en)
WO (1) WO1999046396A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001273454A1 (en) 2000-07-13 2002-01-30 Pioneer Hi-Bred International, Inc. ZmaXIG1 polynucleotides and methods of use
EP1174512A1 (en) * 2000-07-17 2002-01-23 Peter Stamp Seed composition and method for reducing or preventing the release of genetically manipulated pollen
US6646186B1 (en) 2000-07-26 2003-11-11 Stine Seed Farm Inc. Hybrid soybeans and methods of production
CA2420406C (en) 2000-09-29 2014-12-09 Monsanto Technology Llc Glyphosate tolerant wheat plant 33391 and compositions and methods for detection thereof
WO2002045510A2 (en) * 2000-12-07 2002-06-13 D & Pl Technology Holding Corporation Glyphosate-induced male sterility in dicots
DE10163163A1 (en) 2001-12-20 2003-07-03 Basf Ag Process for the production of highly functional, hyperbranched polyester by enzymatic esterification
US7230168B2 (en) 2001-12-20 2007-06-12 The Curators Of The University Of Missouri Reversible male sterility in transgenic plants by expression of cytokinin oxidase
US7939709B2 (en) 2002-02-26 2011-05-10 Syngenta Limited Method for selectively producing male or female sterile plants
ES2382804T3 (en) 2003-02-12 2012-06-13 Monsanto Technology Llc MON 88913 cotton event and compositions and procedures for its detection
WO2004082366A2 (en) * 2003-03-19 2004-09-30 Regents Of The University Of Minnesota Methods to confer enhanced floral properties to plants
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
ZA200803234B (en) * 2005-10-13 2009-01-28 Monsanto Technology Llc Methods for producing hybrid seed
EP3339441A1 (en) 2005-10-13 2018-06-27 Monsanto Technology LLC Methods for producing hybrid seed
EP2059601A2 (en) * 2006-10-03 2009-05-20 Monsanto Technology, LLC Methods for hybrid corn seed production and compositions produced therefrom
EP2985353A1 (en) 2006-10-12 2016-02-17 Monsanto Technology LLC Plant micrornas and methods of use thereof
EP3144391A3 (en) 2009-11-23 2017-06-21 Monsanto Technology LLC Transgenic maize event mon 87427 and the relative development scale
PE20141518A1 (en) 2011-07-01 2014-11-17 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR THE SELECTIVE REGULATION OF PROTEIN EXPRESSION
CN103222423B (en) * 2013-05-14 2014-11-12 安徽省农业科学院水稻研究所 Rice hybrid breeding material as well as cultivation method and application thereof
PT3324732T (en) 2015-07-22 2023-02-14 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
CN112080501B (en) * 2020-09-10 2021-11-23 隆平生物技术(海南)有限公司 Recombinant promoter, gene expression cassette and application of recombinant promoter and gene expression cassette in plant breeding

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ227835A (en) * 1988-02-03 1992-09-25 Paladin Hybrids Inc Antisense gene systems of pollination control for hybrid seed production
AU655945B2 (en) * 1990-08-31 1995-01-19 Monsanto Technology Llc Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases

Also Published As

Publication number Publication date
WO1999046396A3 (en) 1999-10-21
WO1999046396A2 (en) 1999-09-16
AU2992999A (en) 1999-09-27
CN1300324A (en) 2001-06-20
BR9908631A (en) 2000-11-14
EP1062354A2 (en) 2000-12-27

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