WO1999046396A3 - Glyphosate as a gametocide - Google Patents

Glyphosate as a gametocide Download PDF

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Publication number
WO1999046396A3
WO1999046396A3 PCT/US1999/005126 US9905126W WO9946396A3 WO 1999046396 A3 WO1999046396 A3 WO 1999046396A3 US 9905126 W US9905126 W US 9905126W WO 9946396 A3 WO9946396 A3 WO 9946396A3
Authority
WO
WIPO (PCT)
Prior art keywords
glyphosate
gametocide
plants containing
dna molecule
glyphosate tolerance
Prior art date
Application number
PCT/US1999/005126
Other languages
French (fr)
Other versions
WO1999046396A2 (en
Inventor
Sherri M Brown
Michael Ernest Fromm
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Priority to EP99911240A priority Critical patent/EP1062354A2/en
Priority to AU29929/99A priority patent/AU2992999A/en
Priority to BR9908631-0A priority patent/BR9908631A/en
Publication of WO1999046396A2 publication Critical patent/WO1999046396A2/en
Publication of WO1999046396A3 publication Critical patent/WO1999046396A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Glyphosate is used as a highly selective gametocide on plants containing in their genome a first DNA molecule which confers constitutive glyphosate tolerance and a second DNA molecule which inhibits said glyphosate tolerance specifically in male reproductive tissue. Plants containing the first and second DNA molecules are rendered male sterile by exposure to glyphosate. The methods and compositions of the invention are advantageous for use in the generation of hybrid seed, for restricting outcrossing, and for prolonging flower life.
PCT/US1999/005126 1998-03-09 1999-03-09 Glyphosate as a gametocide WO1999046396A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99911240A EP1062354A2 (en) 1998-03-09 1999-03-09 Glyphosate as a gametocide
AU29929/99A AU2992999A (en) 1998-03-09 1999-03-09 Glyphosate as a gametocide
BR9908631-0A BR9908631A (en) 1998-03-09 1999-03-09 Glyphosate as a gameticide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7727798P 1998-03-09 1998-03-09
US60/077,277 1998-03-09

Publications (2)

Publication Number Publication Date
WO1999046396A2 WO1999046396A2 (en) 1999-09-16
WO1999046396A3 true WO1999046396A3 (en) 1999-10-21

Family

ID=22137136

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/005126 WO1999046396A2 (en) 1998-03-09 1999-03-09 Glyphosate as a gametocide

Country Status (6)

Country Link
EP (1) EP1062354A2 (en)
CN (1) CN1300324A (en)
AR (1) AR016185A1 (en)
AU (1) AU2992999A (en)
BR (1) BR9908631A (en)
WO (1) WO1999046396A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9139838B2 (en) 2011-07-01 2015-09-22 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
US9212370B2 (en) 2004-12-21 2015-12-15 Monsanto Technology Llc Recombinant DNA constructs and methods for controlling gene expression

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6838593B2 (en) 2000-07-13 2005-01-04 Pioneer Hi-Bred Int'l Inc. Austin responsive promoter sequences and methods of using the same
EP1174512A1 (en) * 2000-07-17 2002-01-23 Peter Stamp Seed composition and method for reducing or preventing the release of genetically manipulated pollen
US6646186B1 (en) 2000-07-26 2003-11-11 Stine Seed Farm Inc. Hybrid soybeans and methods of production
CZ300073B6 (en) 2000-09-29 2009-01-21 Monsanto Technology Llc Method of improving glyphosate tolerance in a wheat plant, cell of such plant, DNA thereof, a pair of DNA molecules functioning as primers, method of detecting thereof, method of breeding a glyphosate tolerant trait into wheat plants and DNA detectio
AR031645A1 (en) * 2000-12-07 2003-09-24 D & Pl Technology Holding Corp MALE STERILITY IN DICOTILEDONEAS PLANTS, INDICATED BY GLYPHOSATE
US7230168B2 (en) 2001-12-20 2007-06-12 The Curators Of The University Of Missouri Reversible male sterility in transgenic plants by expression of cytokinin oxidase
DE10163163A1 (en) 2001-12-20 2003-07-03 Basf Ag Process for the production of highly functional, hyperbranched polyester by enzymatic esterification
EP2278017B1 (en) 2002-02-26 2015-03-25 Syngenta Limited A method of selectively producing male or female sterile plants
EP2298921B1 (en) 2003-02-12 2016-12-21 Monsanto Technology LLC Cotton event mon 88913 and compositions and methods for detection thereof
WO2004082366A2 (en) * 2003-03-19 2004-09-30 Regents Of The University Of Minnesota Methods to confer enhanced floral properties to plants
WO2007047016A2 (en) 2005-10-13 2007-04-26 Monsanto Technology, Llc Methods for producing hybrid seed
ZA200803234B (en) * 2005-10-13 2009-01-28 Monsanto Technology Llc Methods for producing hybrid seed
BRPI0719815A2 (en) * 2006-10-03 2014-05-20 Monsanto Technology Llc METHODS FOR PRODUCTION OF HYBRID CORN SEED AND COMPOSITIONS PRODUCED FROM THE SAME
EP2074227A4 (en) 2006-10-12 2010-03-10 Monsanto Technology Llc Plant micrornas and methods of use thereof
MX358629B (en) 2009-11-23 2018-08-28 Monsanto Technology Llc Transgenic maize event mon 87427 and the relative development scale.
CN103222423B (en) * 2013-05-14 2014-11-12 安徽省农业科学院水稻研究所 Rice hybrid breeding material as well as cultivation method and application thereof
BR112018001192B1 (en) 2015-07-22 2024-02-06 Monsanto Technology Llc RECOMBINANT DNA MOLECULE, METHOD FOR SELECTIVELY REGULATING THE EXPRESSION OF A PROTEIN IN A MALE REPRODUCTIVE TISSUE OF A TRANSGENIC PLANT, AND USE OF A PLANT OR SEED COMPRISING SAID RECOMBINANT DNA MOLECULE
CN112080501B (en) * 2020-09-10 2021-11-23 隆平生物技术(海南)有限公司 Recombinant promoter, gene expression cassette and application of recombinant promoter and gene expression cassette in plant breeding

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0329308A2 (en) * 1988-02-03 1989-08-23 Pioneer Hi-Bred International, Inc. Antisense gene systems of pollination control for hybrid seed production
WO1992004449A1 (en) * 1990-08-31 1992-03-19 Monsanto Company Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0329308A2 (en) * 1988-02-03 1989-08-23 Pioneer Hi-Bred International, Inc. Antisense gene systems of pollination control for hybrid seed production
WO1992004449A1 (en) * 1990-08-31 1992-03-19 Monsanto Company Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A PE AZOLA-VAZQUEZ ET AL: "Expresson of hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate", PLANT CELL REPORTS, vol. 14, 1 January 1995 (1995-01-01), pages 482 - 487, XP002096051, ISSN: 0721-7714 *
BARRY G ET AL: "INHIBITORS OF AMINO ACID BIOSYNTHESIS: STRATEGIES FOR IMPARTING GLYPHOSATE TOLERANCE TO CROP PLANTS", CURRENT TOPICS IN PLANT PHYSIOLOGY, 1 January 1992 (1992-01-01), pages 139 - 145, XP000566269, ISSN: 1057-6576 *
ZHOU H ET AL: "GLYPHOSATE-TOLERANT CP4 AND GOX GENES AS A SELECTABLE MARKER IN WHEAT TRANSFORMATION", PLANT CELL REPORTS, vol. 15, no. 3/04, 1 December 1995 (1995-12-01), pages 159 - 163, XP000578832, ISSN: 0721-7714 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9212370B2 (en) 2004-12-21 2015-12-15 Monsanto Technology Llc Recombinant DNA constructs and methods for controlling gene expression
US9139838B2 (en) 2011-07-01 2015-09-22 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression

Also Published As

Publication number Publication date
AU2992999A (en) 1999-09-27
WO1999046396A2 (en) 1999-09-16
BR9908631A (en) 2000-11-14
EP1062354A2 (en) 2000-12-27
AR016185A1 (en) 2001-06-20
CN1300324A (en) 2001-06-20

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