AR014061A1 - Polipeptido aislado que presenta actividad enzimatica udp-glucosa deshidrogenasa, proteina de fusion que comprende dicho polipeptido, moleculade acidos nucleicos aislada, vector de expresion, celula huesped que comprende dicho vector de expresion, metodo para producir almidon carboxilado en una - Google Patents

Polipeptido aislado que presenta actividad enzimatica udp-glucosa deshidrogenasa, proteina de fusion que comprende dicho polipeptido, moleculade acidos nucleicos aislada, vector de expresion, celula huesped que comprende dicho vector de expresion, metodo para producir almidon carboxilado en una

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Publication number
AR014061A1
AR014061A1 ARP980106277A ARP980106277A AR014061A1 AR 014061 A1 AR014061 A1 AR 014061A1 AR P980106277 A ARP980106277 A AR P980106277A AR P980106277 A ARP980106277 A AR P980106277A AR 014061 A1 AR014061 A1 AR 014061A1
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AR
Argentina
Prior art keywords
plant
polypeptide
amino acid
udp
udpgdh
Prior art date
Application number
ARP980106277A
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English (en)
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Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of AR014061A1 publication Critical patent/AR014061A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un polipéptido aislado que presenta actividad enzimática UDP-glucosa deshidrogenasa (UDPGdH), donde dicho polipéptido posee una secuencia deaminoácidos seleccionado del grupo formado por (a) una secuencia de aminoácidos que posee por lo menos un 80% de identidad con la secuencia deaminoácidos de la SEQ ID N*: 2 o SEQ ID N*: 4, con la condicion de que dicha secuencia de aminoácidos no sea codificada por el gen UDPGdH de soja cuyasecuencia de nucleotidos tiene el N* de Accesode GenBank U53418 y (b) un fragmento funcional de (a). La invencion atane a moléculas de ácido nucleicoque comprenden dichas secuencias y formas mutantes codificadas de UDPGdH donde los residuos de cisteína 272 han sido sustituidos porresiduos de otroaminoácido. Estas encimas mutantes mayormente catalizan la conversion de UDP-D-glucosa en UDP-6-aldehído-D-glucosa como producto final. La invencionprovee vectores que comprenden dichas secuencias de nucleicos, proteínas defusion que comprenden dichos polipéptidos y células huésped que contienendichos vectores. Otro asunto de la invencion son el método para producir una planta que exprese una UDPGdH y una planta transgénica. Además otros asuntosson el método para producir almidon carboxilado en una planta, el método para producir almidon aldehídico en una planta, el método para obtener unapreparacion de almidon vegetal, el método para producir células vegetales, el método para incrementar la biosíntesis degomas en semillas, el método paraincrementar capacidad de extraccion de almidon en procesos de molienda humeda de maíz, el método para incrementar el valor nutricional de plantas o desemillas, el método para incrementar la resistencia de tallos ypecíolos de plantas, el método para incrementar la resistencia a insectos y patogenos
ARP980106277A 1997-12-10 1998-12-10 Polipeptido aislado que presenta actividad enzimatica udp-glucosa deshidrogenasa, proteina de fusion que comprende dicho polipeptido, moleculade acidos nucleicos aislada, vector de expresion, celula huesped que comprende dicho vector de expresion, metodo para producir almidon carboxilado en una AR014061A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/987,367 US6399859B1 (en) 1997-12-10 1997-12-10 Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof

Publications (1)

Publication Number Publication Date
AR014061A1 true AR014061A1 (es) 2001-01-31

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ARP980106277A AR014061A1 (es) 1997-12-10 1998-12-10 Polipeptido aislado que presenta actividad enzimatica udp-glucosa deshidrogenasa, proteina de fusion que comprende dicho polipeptido, moleculade acidos nucleicos aislada, vector de expresion, celula huesped que comprende dicho vector de expresion, metodo para producir almidon carboxilado en una

Country Status (7)

Country Link
US (2) US6399859B1 (es)
EP (1) EP1038005A2 (es)
AR (1) AR014061A1 (es)
AU (1) AU2086199A (es)
CA (1) CA2313446A1 (es)
WO (1) WO1999029875A2 (es)
ZA (1) ZA9811355B (es)

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AU1368199A (en) 1997-10-30 1999-05-24 Board Of Regents Of The University And Community College System Of Nevada, The The uridine diphosphate glucose dehydrogenase gene and its role in altering plant growth and plant characteristics

Also Published As

Publication number Publication date
US20040025203A1 (en) 2004-02-05
AU2086199A (en) 1999-06-28
EP1038005A2 (en) 2000-09-27
ZA9811355B (en) 1999-06-10
WO1999029875A3 (en) 1999-08-12
US7098381B2 (en) 2006-08-29
CA2313446A1 (en) 1999-06-17
US6399859B1 (en) 2002-06-04
WO1999029875A2 (en) 1999-06-17

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