AR013989A1 - Fragmento aislado de acidos nucleicos que codifica enzimas que regulan la biosintesis y utilizacion de amonoacidos ramificados en semillas y plantas, ungen quimerico, una celula huesped transformada, un polipeptido, un metodo para alterar el nivel de expresion de dichas enzimas, metodos de obtencion - Google Patents

Fragmento aislado de acidos nucleicos que codifica enzimas que regulan la biosintesis y utilizacion de amonoacidos ramificados en semillas y plantas, ungen quimerico, una celula huesped transformada, un polipeptido, un metodo para alterar el nivel de expresion de dichas enzimas, metodos de obtencion

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Publication number
AR013989A1
AR013989A1 ARP980105410A ARP980105410A AR013989A1 AR 013989 A1 AR013989 A1 AR 013989A1 AR P980105410 A ARP980105410 A AR P980105410A AR P980105410 A ARP980105410 A AR P980105410A AR 013989 A1 AR013989 A1 AR 013989A1
Authority
AR
Argentina
Prior art keywords
biosynthesis
enzymes
nucleic acids
ammonocacies
chemeric
Prior art date
Application number
ARP980105410A
Other languages
English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR013989A1 publication Critical patent/AR013989A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1096Transferases (2.) transferring nitrogenous groups (2.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/948Microorganisms using viruses or cell lines

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se revelan fragmentos aislados de ácidos nucleicos que codifican enzimas vegetales involucradas en la bioséntesis de los aminoácidos ramificados y desus porciones. Estas enzimas son dihidroxiácido deshidratasa, distintas isoenzimas de la aminotransferasa de aminoácidos de cadena ramificada, lasubunidad leuC de la 3-isopropilmalato deshidratasa y la subunidad leuD de la 3-isopropilmalato deshidratasa. También se describen genes quiméricos quecontienen los fragmentos de ácidos nucleicos antes mencionados y células huesped transformadas con estos genes quiméricos. Los polipéptidos codificadospor estos fragmentos de ácidos nucleicos y los métodos para allterar su nivel de expresion, también se incluyen. Asimismo se describen composiciones quecomprenden dicho polinucleotido y dicho polipéptido. Se revelan también, los métodos para obtener polinucleotidos que codifican enzimas vegetalesinvolucradas en la biosíntesis de los aminoácidos ramificados y finalmente un método para evaluar la capacidad de un compuesto para inhibir la actividad deuna enzima vegetal, involucrada en la biosíntesis de los aminoácidos ramificados.
ARP980105410A 1997-10-28 1998-10-28 Fragmento aislado de acidos nucleicos que codifica enzimas que regulan la biosintesis y utilizacion de amonoacidos ramificados en semillas y plantas, ungen quimerico, una celula huesped transformada, un polipeptido, un metodo para alterar el nivel de expresion de dichas enzimas, metodos de obtencion AR013989A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6342397P 1997-10-28 1997-10-28

Publications (1)

Publication Number Publication Date
AR013989A1 true AR013989A1 (es) 2001-01-31

Family

ID=22049097

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980105410A AR013989A1 (es) 1997-10-28 1998-10-28 Fragmento aislado de acidos nucleicos que codifica enzimas que regulan la biosintesis y utilizacion de amonoacidos ramificados en semillas y plantas, ungen quimerico, una celula huesped transformada, un polipeptido, un metodo para alterar el nivel de expresion de dichas enzimas, metodos de obtencion

Country Status (6)

Country Link
US (2) US6451581B1 (es)
EP (1) EP1025210A2 (es)
AR (1) AR013989A1 (es)
AU (1) AU1190999A (es)
BR (1) BR9815228A (es)
WO (1) WO1999021880A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3783777B2 (ja) * 2002-09-20 2006-06-07 独立行政法人農業生物資源研究所 新たな機能を持つジベレリン2−酸化酵素遺伝子およびその利用
US7150745B2 (en) * 2004-01-09 2006-12-19 Barrx Medical, Inc. Devices and methods for treatment of luminal tissue
CA2617734A1 (en) * 2005-08-12 2007-02-22 Suntory Limited Dihydroxy-acid dehydratase gene and use thereof
US20140216118A1 (en) 2011-06-14 2014-08-07 Synthon Biopharmaceuticals B.V. Compositions and Methods for Making and Biocontaining Auxotrophic Transgenic Plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2696190B1 (fr) 1992-09-25 1994-12-09 Agronomique Inst Nat Rech Acide nucléique codant pour une alpha-acétolactate synthase et ses applications.

Also Published As

Publication number Publication date
WO1999021880A2 (en) 1999-05-06
US6451581B1 (en) 2002-09-17
BR9815228A (pt) 2001-01-02
AU1190999A (en) 1999-05-17
WO1999021880A3 (en) 1999-11-25
US6803223B2 (en) 2004-10-12
US20020102715A1 (en) 2002-08-01
EP1025210A2 (en) 2000-08-09

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