AR012445A1 - Metodo de produccion de una planta transgenica y de cultivo; acido nucleico aislado, enriquecido, libre, de celulas y/o recombinante de utilidad para laproduccion de dicha planta y celula vegetal que lo comprende. - Google Patents

Metodo de produccion de una planta transgenica y de cultivo; acido nucleico aislado, enriquecido, libre, de celulas y/o recombinante de utilidad para laproduccion de dicha planta y celula vegetal que lo comprende.

Info

Publication number
AR012445A1
AR012445A1 ARP980101750A ARP980101750A AR012445A1 AR 012445 A1 AR012445 A1 AR 012445A1 AR P980101750 A ARP980101750 A AR P980101750A AR P980101750 A ARP980101750 A AR P980101750A AR 012445 A1 AR012445 A1 AR 012445A1
Authority
AR
Argentina
Prior art keywords
plant
ferritin
transgenic plant
transgenic
biotic
Prior art date
Application number
ARP980101750A
Other languages
English (en)
Original Assignee
British Tech Group
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by British Tech Group filed Critical British Tech Group
Publication of AR012445A1 publication Critical patent/AR012445A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un método de produccion de una planta transgénica que comprende trasnformar una célula de una planta de origen con un ácido nucleico codificado paraexpresar una ferritina de modo tal que la planta o una parte de la misma derivada de dicha célula sobreproduzca la ferritina suficiente como paraproveer una reduccion significativa en la necrotizacion y/o reduccion en la pérdida permanente de la intesidad fotosintética cuando se la exponga a unesfuerzo biotico o abiotico con relacion a laplanta de origen. La ferritina posee homología de por lo menos 90% en la secuencia de aminoácidos con la ID deSEC. Nro 2. Las partes de la planta transgénica obtenida demuestran una significativa reduccion en la necrotizacion cuando se lasexpone a un esfuerzoabiotico o biotico y la intensidad de las reacciones fotosintéticas en las hojas de la planta transgénica no disminuye en más de 10% luego de untratamiento de 70 horas con 10 omega M de paraquat, o bien no disminuye. Laplanta transgénica posee también una resistencia mejorada contra el tratamientocon ácido furásico y/o las infecciones de origen viral y/o bacteriano y/o fungal y la ferritina se expresa ectopicamente en los tejidos vegetales de laplanta trasngénica. La proteína se expresa bajo el control de un promotor que comprende al promotor genético de subunidades pequenas ribulosa-1,5-bifosfatocarboxilasa y la ferritina se encuentra truncada en la region de tránsito N-terminal. Un método de produccion de un cultivo que comprende la obtencion deuna planta transgénica mediante el método de acuerdo con lo descripto y su desarrollo bajo condiciones sujetas a esfuerzo abiotico o biotico y dichoesfuerzo biotico se provee mediante la exposicion a virus, bacterias, hongos y/o insectos, o bien mediante una elevada intensidad lumínica, radiacion UV-B,
ARP980101750A 1997-04-16 1998-04-16 Metodo de produccion de una planta transgenica y de cultivo; acido nucleico aislado, enriquecido, libre, de celulas y/o recombinante de utilidad para laproduccion de dicha planta y celula vegetal que lo comprende. AR012445A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU9700762A HUP9700762A3 (en) 1997-04-16 1997-04-16 Transgenic stressresistant plants overexpressing iron-binding ferritin protein

Publications (1)

Publication Number Publication Date
AR012445A1 true AR012445A1 (es) 2000-10-18

Family

ID=89575031

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980101750A AR012445A1 (es) 1997-04-16 1998-04-16 Metodo de produccion de una planta transgenica y de cultivo; acido nucleico aislado, enriquecido, libre, de celulas y/o recombinante de utilidad para laproduccion de dicha planta y celula vegetal que lo comprende.

Country Status (14)

Country Link
US (2) US6563019B1 (es)
EP (1) EP0975779A1 (es)
JP (1) JP2001519671A (es)
CN (1) CN1252839A (es)
AR (1) AR012445A1 (es)
AU (1) AU750821B2 (es)
BR (1) BR9808439A (es)
CA (1) CA2283750A1 (es)
EE (1) EE9900447A (es)
HU (1) HUP9700762A3 (es)
IL (1) IL131580A0 (es)
NZ (1) NZ337463A (es)
WO (1) WO1998046775A1 (es)
ZA (1) ZA983196B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3331367B2 (ja) * 1997-03-11 2002-10-07 独立行政法人農業生物資源研究所 細胞死抑制遺伝子が導入されたストレス抵抗性植物およびその作出方法
GB9917642D0 (en) * 1999-07-27 1999-09-29 Zeneca Ltd Improvements in or relating to organic compounds
GB0021879D0 (en) * 2000-09-06 2000-10-18 Univ Edinburgh Plant resistance gene
CA2437819A1 (en) * 2001-02-14 2002-08-22 Ventria Bioscience Feed additive compositions and methods
JP4312012B2 (ja) 2003-09-12 2009-08-12 トヨタ自動車株式会社 パラコート（登録商標）耐性遺伝子並びに維管束及びトライコーム特異的プロモーター
US7772464B2 (en) * 2005-05-20 2010-08-10 Cal/West Seeds Agronomically adapted alfalfa plants with high levels of somatic embryogenesis
WO2007141808A2 (en) * 2006-06-08 2007-12-13 Niranjan Chakraborty Extra-cellular matrix localized ferritin for iron uptake, storage, and stress tolerance
US8163977B2 (en) 2006-06-08 2012-04-24 Niranjan Chakraborty Extra-cellular matrix localized ferritin for iron uptake, storage, and stress tolerance
CN112457382B (zh) * 2020-12-14 2021-12-07 南京林业大学 一种紫花苜蓿植物铁蛋白的表达纯化方法及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2256856T3 (es) 1995-04-06 2006-07-16 Seminis Vegetable Seeds, Inc. Proceso de seccion de celulas transgenicas.
JPH09140384A (ja) * 1995-11-20 1997-06-03 Netsutairin Saisei Gijutsu Kenkyu Kumiai 酸性土壌で誘導される植物のタンパク質遺伝子
JPH09201190A (ja) * 1996-01-23 1997-08-05 Central Res Inst Of Electric Power Ind 分子育種法による高鉄含量植物およびその作出方法

Also Published As

Publication number Publication date
CN1252839A (zh) 2000-05-10
AU7062098A (en) 1998-11-11
JP2001519671A (ja) 2001-10-23
CA2283750A1 (en) 1998-10-22
US20010039670A1 (en) 2001-11-08
HUP9700762A2 (hu) 1999-02-01
EP0975779A1 (en) 2000-02-02
EE9900447A (et) 2000-04-17
WO1998046775A1 (en) 1998-10-22
AU750821B2 (en) 2002-08-01
IL131580A0 (en) 2001-01-28
ZA983196B (en) 1999-10-18
HUP9700762A3 (en) 2000-08-28
BR9808439A (pt) 2000-05-23
HU9700762D0 (en) 1997-05-28
US6563019B1 (en) 2003-05-13
NZ337463A (en) 2001-03-30

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