AR000606A1 - An expression vector for a bovine growth hormone cellulose e.coli transformed with said vector and a process for the mass production of the hormone by using an e.coli transformant - Google Patents

An expression vector for a bovine growth hormone cellulose e.coli transformed with said vector and a process for the mass production of the hormone by using an e.coli transformant

Info

Publication number
AR000606A1
AR000606A1 AR33489496A AR33489496A AR000606A1 AR 000606 A1 AR000606 A1 AR 000606A1 AR 33489496 A AR33489496 A AR 33489496A AR 33489496 A AR33489496 A AR 33489496A AR 000606 A1 AR000606 A1 AR 000606A1
Authority
AR
Argentina
Prior art keywords
hormone
vector
coli
mass production
bovine growth
Prior art date
Application number
AR33489496A
Other languages
Spanish (es)
Original Assignee
Lg Chemical Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lg Chemical Ltd filed Critical Lg Chemical Ltd
Publication of AR000606A1 publication Critical patent/AR000606A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un vector expresión que comprende un gen de una hormona de crecimiento bovino (BGH), en donde la secuencia de nucleótido en la región de terminal5 es cambiada sin cambiar la secuencia de aminoácido codificada en la misma, y un terminador detran scripción trpA insertado luego del terminal 3 del gen BGHmodificado es utilizado para transformar una célula de E.Coli para la producción en masa de BGH.An expression vector comprising a bovine growth hormone (BGH) gene, wherein the nucleotide sequence in the terminal5 region is changed without changing the amino acid sequence encoded therein, and a trpA detranscription terminator inserted after the Terminal 3 of the modified BGH gene is used to transform an E.Coli cell for the mass production of BGH.

AR33489496A 1994-12-30 1996-01-02 An expression vector for a bovine growth hormone cellulose e.coli transformed with said vector and a process for the mass production of the hormone by using an e.coli transformant AR000606A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940040025A KR100225511B1 (en) 1994-12-30 1994-12-30 Process for the mass production of bovine growth hormone

Publications (1)

Publication Number Publication Date
AR000606A1 true AR000606A1 (en) 1997-07-10

Family

ID=36951464

Family Applications (1)

Application Number Title Priority Date Filing Date
AR33489496A AR000606A1 (en) 1994-12-30 1996-01-02 An expression vector for a bovine growth hormone cellulose e.coli transformed with said vector and a process for the mass production of the hormone by using an e.coli transformant

Country Status (10)

Country Link
JP (1) JP3234478B2 (en)
KR (1) KR100225511B1 (en)
AR (1) AR000606A1 (en)
AU (1) AU713218B2 (en)
BG (1) BG64565B1 (en)
BR (1) BR9600009A (en)
CO (1) CO4480064A1 (en)
NZ (1) NZ280764A (en)
TW (1) TW505694B (en)
ZA (1) ZA9511030B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182318A (en) * 2018-08-16 2019-01-11 湖北大学 A method of utilizing pBV220 expression vector high efficient expression tryptophan synthetase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0712318B2 (en) * 1986-12-31 1995-02-15 株式会社ラッキー Method for producing bovine growth hormone using synthetic gene
DE4101736A1 (en) * 1991-01-22 1992-07-23 Gruenenthal Gmbh NEW PLASMINOGENACTIVATORS USEFUL POLYPEPTIDES, DAFUER CODING PLASMIDS AND METHOD FOR THE PRODUCTION THEREOF AND THEIR USE

Also Published As

Publication number Publication date
AU4076895A (en) 1996-07-11
JP3234478B2 (en) 2001-12-04
NZ280764A (en) 1997-02-24
ZA9511030B (en) 1996-07-09
CO4480064A1 (en) 1997-07-09
AU713218B2 (en) 1999-11-25
BG64565B1 (en) 2005-07-29
JPH08228787A (en) 1996-09-10
BG100264A (en) 1996-12-31
TW505694B (en) 2002-10-11
KR960023059A (en) 1996-07-18
KR100225511B1 (en) 1999-10-15
BR9600009A (en) 1998-01-21

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