KR960023059A - Method of mass production of bovine growth hormone - Google Patents

Method of mass production of bovine growth hormone Download PDF

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Publication number
KR960023059A
KR960023059A KR1019940040025A KR19940040025A KR960023059A KR 960023059 A KR960023059 A KR 960023059A KR 1019940040025 A KR1019940040025 A KR 1019940040025A KR 19940040025 A KR19940040025 A KR 19940040025A KR 960023059 A KR960023059 A KR 960023059A
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KR
South Korea
Prior art keywords
growth hormone
bovine growth
expression vector
gene
coli
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Application number
KR1019940040025A
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Korean (ko)
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KR100225511B1 (en
Inventor
김천형
조중명
Original Assignee
성재갑
주식회사 Lg 화학
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 성재갑, 주식회사 Lg 화학 filed Critical 성재갑
Priority to KR1019940040025A priority Critical patent/KR100225511B1/en
Priority to NZ280764A priority patent/NZ280764A/en
Priority to CO95062000A priority patent/CO4480064A1/en
Priority to ZA9511030A priority patent/ZA9511030B/en
Priority to BG100264A priority patent/BG64565B1/en
Priority to AU40768/95A priority patent/AU713218B2/en
Priority to JP34263295A priority patent/JP3234478B2/en
Priority to BR9600009A priority patent/BR9600009A/en
Priority to AR33489496A priority patent/AR000606A1/en
Priority to TW085106241A priority patent/TW505694B/en
Publication of KR960023059A publication Critical patent/KR960023059A/en
Application granted granted Critical
Publication of KR100225511B1 publication Critical patent/KR100225511B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 5'-말단부분의 염기서열이 그에 코드되는 아미노산 서열의 변화없이 변형된 소 성장호르몬 유전자와 상기 유전자의 3'-말단에 연결된 trpA 전이 터미네이터를 포함함을 특징으로 하는 소 성장 호르몬 발현 벡터, 이에 의해 형질전환된 대장균 및 이를 이용하여 높은 수율로 소 성장 호르몬을 제조하는 방법에 관한 것이다.The present invention provides a bovine growth hormone expression comprising a bovine growth hormone gene modified at 5′-terminal sequence without modification of the amino acid sequence encoded therein and a trpA transfer terminator linked to the 3′-end of the gene. A vector, thereby transformed E. coli and a method for producing bovine growth hormone in high yield using the same.

Description

소 성장 호르몬의 대량 생산 방법Method of mass production of bovine growth hormone

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 소 성장 호르몬 유전자의 5' 말단 부분과 이를 변형시킨 염기 서열을 비교한 것이고,1 is a comparison of the 5 'terminal portion of the bovine growth hormone gene with the nucleotide sequence modified therefrom.

제2도는 소 성장 호르몬 유전자의 5'말단에 변형된 유전자 염기 서열을, 3'말단 뒤에 trpA 전이 터미네이터를 접합하는 전략 및 이를 포함하는 발현 벡터의 제조과정을 도시한 것이다.Figure 2 shows a strategy for conjugating the trpA transition terminator after the modified gene base sequence at the 5 'end of the bovine growth hormone gene 3' end, and the manufacturing process of the expression vector comprising the same.

Claims (7)

5'-말단부분의 염기서열이 그에 코드되는 아미노산 서열의 변화없이 변형된 소 성장 호르몬 유전자와 상기 유전자의 3'-말단에 연결된 trpA 전이 터미네이터를 포함함을 특징으로 하는 소 성장 호르몬 발현 벡터.A bovine growth hormone expression vector comprising a bovine growth hormone gene modified at 5′-terminal sequence without a change in the amino acid sequence encoded therein and a trpA transition terminator linked to the 3′-end of the gene. 제1항에 있어서, 상기 소 성장 호르몬 유전자의 5'-말단 부분이 다음의 염기 서열을 가짐을 특징으로 하는 발현 벡터:The expression vector of claim 1, wherein the 5′-terminal portion of the bovine growth hormone gene has the following nucleotide sequence: 5'-GCT TTT CCG GCT ATG TCT CTA TCT GGC CTA TTC GCA AAT GCC GTT CTT CGA GCT CAG CAT CTT CAT CAS CTG GCT-3'5'- GCT TTT CCG GCT ATG TCT CTA TCT GGC CTA TTC GCA AAT GCC GTT CTT CGA GCT CAG CAT CTT CAT CAS CTG GCT-3 ' (상기에서 ATG는 소 성장 호르몬 유전자의 번역 개시 코돈을 가리킨다.)(ATG above refers to the translation initiation codon of the bovine growth hormone gene.) 제1항 또는 제2항에 있어서, 상기 trpA 전이 터미네이터가 하기 염기 서열을 가짐을 특징으로 하는 발현 벡터:The expression vector according to claim 1 or 2, wherein the trpA transition terminator has the following nucleotide sequence: 5'-AGCCCGCCTAATGAGCGGGCTTTTTTTT-3'5'-AGCCCGCCTAATGAGCGGGCTTTTTTTT-3 ' 제3항에 있어서, 발현 벡터 ptrp 3H BGHRAN.The expression vector ptrp 3H BGHRAN of claim 3. 제1항 내지 제4항 중 어느 한 항의 발현 벡터로 형질전환된 대장균.E. coli transformed with the expression vector of any one of claims 1 to 4. 제5항에 있어서, 벡터 ptrp 3H BGHRAN에 의해 형질전환된 대장균 W3110(KCTC 0143BP).The E. coli W3110 (KCTC 0143BP) transformed by the vector ptrp 3H BGHRAN. 제5항 또는 제6항의 대장균을 배양한 다음, 소 성장 호르몬을 분리함을 특징으로 하는 소 성장 호르몬의 제조 방법.A method for producing bovine growth hormone, characterized in that after culturing E. coli of claim 5 or 6, bovine growth hormone is isolated. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940040025A 1994-12-30 1994-12-30 Process for the mass production of bovine growth hormone KR100225511B1 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
KR1019940040025A KR100225511B1 (en) 1994-12-30 1994-12-30 Process for the mass production of bovine growth hormone
NZ280764A NZ280764A (en) 1994-12-30 1995-12-21 Expression vector comprising bovine growth hormone gene modified at the 5'end region without changing the encoded protein sequence and a trpa transcription terminator
ZA9511030A ZA9511030B (en) 1994-12-30 1995-12-28 Process for the mass production of bovine growth hormone
BG100264A BG64565B1 (en) 1994-12-30 1995-12-28 Vector for bovine growth hormone expression and method for its mass production
CO95062000A CO4480064A1 (en) 1994-12-30 1995-12-28 PROCESS FOR THE MASS PRODUCTION OF GROWTH HORMONES IN CATTLE
AU40768/95A AU713218B2 (en) 1994-12-30 1995-12-28 Process for the mass production of bovine growth hormone
JP34263295A JP3234478B2 (en) 1994-12-30 1995-12-28 Mass production of bovine growth hormone
BR9600009A BR9600009A (en) 1994-12-30 1996-01-02 Expression vector for bovine growth hormone transformed entamoeba coli cell and process for mass production of bovine growth hormone
AR33489496A AR000606A1 (en) 1994-12-30 1996-01-02 An expression vector for a bovine growth hormone cellulose e.coli transformed with said vector and a process for the mass production of the hormone by using an e.coli transformant
TW085106241A TW505694B (en) 1994-12-30 1996-05-25 Process for the mass production of bovine growth hormone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940040025A KR100225511B1 (en) 1994-12-30 1994-12-30 Process for the mass production of bovine growth hormone

Publications (2)

Publication Number Publication Date
KR960023059A true KR960023059A (en) 1996-07-18
KR100225511B1 KR100225511B1 (en) 1999-10-15

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KR1019940040025A KR100225511B1 (en) 1994-12-30 1994-12-30 Process for the mass production of bovine growth hormone

Country Status (10)

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JP (1) JP3234478B2 (en)
KR (1) KR100225511B1 (en)
AR (1) AR000606A1 (en)
AU (1) AU713218B2 (en)
BG (1) BG64565B1 (en)
BR (1) BR9600009A (en)
CO (1) CO4480064A1 (en)
NZ (1) NZ280764A (en)
TW (1) TW505694B (en)
ZA (1) ZA9511030B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182318A (en) * 2018-08-16 2019-01-11 湖北大学 A method of utilizing pBV220 expression vector high efficient expression tryptophan synthetase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0712318B2 (en) * 1986-12-31 1995-02-15 株式会社ラッキー Method for producing bovine growth hormone using synthetic gene
DE4101736A1 (en) * 1991-01-22 1992-07-23 Gruenenthal Gmbh NEW PLASMINOGENACTIVATORS USEFUL POLYPEPTIDES, DAFUER CODING PLASMIDS AND METHOD FOR THE PRODUCTION THEREOF AND THEIR USE

Also Published As

Publication number Publication date
BG64565B1 (en) 2005-07-29
TW505694B (en) 2002-10-11
NZ280764A (en) 1997-02-24
JP3234478B2 (en) 2001-12-04
BG100264A (en) 1996-12-31
AU713218B2 (en) 1999-11-25
KR100225511B1 (en) 1999-10-15
JPH08228787A (en) 1996-09-10
AR000606A1 (en) 1997-07-10
CO4480064A1 (en) 1997-07-09
ZA9511030B (en) 1996-07-09
BR9600009A (en) 1998-01-21
AU4076895A (en) 1996-07-11

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