US20240042427A1 - Specimen container and centrifugation method for separating serum or plasma from whole blood therewith - Google Patents
Specimen container and centrifugation method for separating serum or plasma from whole blood therewith Download PDFInfo
- Publication number
- US20240042427A1 US20240042427A1 US18/323,830 US202318323830A US2024042427A1 US 20240042427 A1 US20240042427 A1 US 20240042427A1 US 202318323830 A US202318323830 A US 202318323830A US 2024042427 A1 US2024042427 A1 US 2024042427A1
- Authority
- US
- United States
- Prior art keywords
- specimen
- cap
- tube
- reservoir
- specimen tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002966 serum Anatomy 0.000 title claims abstract description 65
- 210000004369 blood Anatomy 0.000 title claims abstract description 51
- 239000008280 blood Substances 0.000 title claims abstract description 51
- 238000005119 centrifugation Methods 0.000 title claims description 26
- 210000000601 blood cell Anatomy 0.000 claims abstract description 66
- 210000002381 plasma Anatomy 0.000 claims abstract description 64
- 230000009974 thixotropic effect Effects 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 13
- 239000007787 solid Substances 0.000 description 23
- 230000005484 gravity Effects 0.000 description 12
- 238000002405 diagnostic procedure Methods 0.000 description 8
- 230000007246 mechanism Effects 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 230000004888 barrier function Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052744 lithium Inorganic materials 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
- A61B5/154—Devices using pre-evacuated means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
Definitions
- the present invention relates to a device and method for separating serum or plasma from whole blood.
- Serum can be separated from whole blood by first allowing the blood to clot and then centrifuging the specimen to move all the blood cells to the bottom of the specimen tube.
- Plasma can be separated from whole blood by first mixing the whole blood with an anticoagulant such as lithium heparin or potassium EDTA and then centrifuging the specimen to move all the blood cells to the bottom of the specimen tube.
- centrifugation When using centrifugation to separate serum or plasma from whole blood, it is common to use a specimen tube that contains a thixotropic gel which has a greater specific gravity than serum or plasma, but lower specific gravity than blood cells. During centrifugation, this gel migrates above the blood cells while staying below the serum or plasma. The function of the gel is to provide a barrier between the serum or plasma and the blood cells so that the serum or plasma do not remix with the blood cells after centrifugation.
- the term “dead volume” refers to the amount of unusable sample left in the specimen tube after the maximum amount of sample has been extracted.
- the pipette must be kept a safe depth above the blood cells or gel separator to ensure it does not make contact with the blood cells or the gel separator.
- the serum or plasma which is above the blood cells or the gel separator yet below the pipette tip is unusable as it won't be extracted in the pipette.
- dead volume may not be problematic when the amount of serum or plasma available is significantly greater than the amount required by the diagnostic tests. However, as the amount of serum or plasma required by the diagnostic tests approaches the amount of serum or plasma available, dead volume becomes a greater concern. This is particularly important when using small volume sample collection technologies or in pediatric samples where the amount of blood able to be drawn is more limited.
- one common technique to reduce the dead volume left when pipetting from a centrifuged specimen container is to pour the serum or plasma out of the centrifuged specimen tube, into a secondary specimen tube. Because the secondary specimen tube does not contain blood cells or gel separator, a pipette can safely dive to the bottom of the specimen tube and the serum or plasma can be extracted with a low dead volume. While this technique results in a low dead volume, there are several significant disadvantages. This technique consumes an additional specimen container, which results in added material costs. The step of pouring serum or plasma increases labor costs and introduces risk of human error. There is also a risk of specimen mix-up if the new specimen tube is not properly labeled.
- the present invention uses a cap with a reservoir, such that blood cells are packed into the cap when a specimen tube is centrifuged with the capped end away from the axis of centrifugation. When the cap is removed, the blood cells are also removed, so that the serum or plasma is left in the specimen tube where it can be readily extracted by a pipette which is able to reach all the way to the bottom of the specimen tube minimizing the dead volume.
- FIG. 1 A- 1 C show a cross-sectional view of the specimen tube and cap according to the invention.
- FIG. 2 shows a cross-sectional view of the specimen container according to the invention.
- FIG. 3 A- 3 I depicts the methodology for separating serum or plasma from blood cells according to the invention.
- FIGS. 4 A and 4 B depicts a preferred embodiment of the specimen container according to the present invention.
- FIG. 1 depicts a specimen tube, often called a test tube, in which a blood specimen is collected according to the present invention.
- the specimen tube 1 has a closed end 3 , open end 4 and lateral wall(s) 2 .
- the open end 4 enables a liquid specimen to be inserted into the specimen tube 1 .
- the closed end 3 is shown in a preferred embodiment with a round bottom.
- Specimen tubes having a round or conical bottom are preferred as they minimize dead volume when pipetting from the bottom of the specimen tube. While specimen tubes with round or conical bottoms are preferred, the bottom of the specimen tube can be any shape.
- FIG. 1 B depicts a cap 10 to secure onto a specimen tube such as that shown in FIG. 1 A .
- the cap 10 has an open end 12 , a closed end 13 and a lateral wall 14 .
- the closed end 13 is closed by a surface 15 .
- the cap 10 has a reservoir 16 which is formed when the cap 10 and specimen tube 1 are secured to formed a specimen container comprising the specimen tube and the cap, as shown in FIG. 2 , where the volume of the reservoir 16 is defined as the maximum amount of liquid that can be contained in the cap 10 without coming into contact with the specimen tube 1 .
- the volume of the reservoir 16 should be equal to or greater than the volume of the blood cells and other solids to be separated from the serum or plasma in the blood specimen. This will enable to the blood cells and other solids to be removed when the cap 10 is removed from the specimen tube 1 .
- the cap is sized so that volume of the reservoir is equal to or greater than the volume of whole blood in the specimen tube. This design will decrease or eliminate the pressure on the seal between the specimen tube and the cap during centrifugation.
- the specimen tube 1 and cap 10 have a mechanism by which the open end 4 of the specimen tube 1 may be secured with the open end 12 of the cap 10 to form a specimen container, such that the blood specimen is contained within the specimen tube 1 and the cap 10 .
- the mechanism for securing the specimen tube 1 and the cap 10 can be any mechanism desired as long as the specimen tube and cap are secured to create a leak free fitting. It is important that the contents of the specimen container do not leak when the specimen container is oriented in any direction.
- Such mechanisms for securing the specimen tube to the cap can include an engineered fit or interference fit.
- a preferred engineered fit is a threaded connection 7 where the specimen tube and the cap both have screw threads that work to connect the specimen tube and the cap together as shown in the figures.
- any other engineered fit can be used that creates a leak free locking mechanism.
- an interference fit can be used such that the specimen tube and the cap are secured by friction after the open end of the specimen tube and open end of the cap are pushed together.
- the cap can be a plug that is secured onto the open end of the specimen tube.
- the joint between the specimen tube and the cap can be sealed using a gasket.
- the mechanism to secure the specimen tube and cap together is not limited to any particular mechanism and shall include any mechanism whereby the specimen tube and cap can be secured together to create a leak free specimen container.
- FIGS. 3 A- 3 I depict an embodiment of the inventive method to separate serum or plasma from a blood specimen and works as follows:
- FIG. 3 A an empty specimen tube 20 having a closed end 21 , open end 22 and lateral wall(s) 23 is shown.
- FIG. 3 B shows the specimen tube 20 filled with a whole blood specimen 24 which was inserted through open end 22 .
- a cap 30 such as that shown in FIG. 1 B , is secured to the open end 22 of the specimen tube 20 .
- a specimen tube that is enclosed with a cap is referred to herein as a specimen container.
- the cap has an open end 32 , a closed end 33 and a lateral wall 34 .
- the cap 30 contains a separator 31 .
- a separator is a device that is put into a specimen tube (or alternatively, into a cap) in order to ensure that after the specimen tube is centrifuged, there is a physical layer separating the serum or plasma from the blood cells and other solids.
- the separator is chosen such that it has a higher specific gravity than serum and/or plasma and lower specific gravity than blood cells and other solids in the blood to be removed.
- the serum or plasma is separated from blood cells and any other solids in the blood by migrating closer to the axis of centrifugation because it has lower specific gravity.
- the blood cells (and other solids) migrate further away from the axis of centrifugation because they have higher specific gravity.
- the separator migrates to a level between the serum or plasma and the blood cells (and other solids), because it has intermediate specific gravity.
- the primary function of the separator is to maintain the purity of the serum or plasma by 1) preventing the serum or plasma from remixing with the blood cells, and 2) preventing the serum or plasma from becoming contaminated by the blood cells as they degrade.
- a preferred separator is a thixotropic gel. This is a hydrophobic gel which is initially solid, but becomes liquefied during centrifugation so that it can migrate to form a layer between the serum or plasma and the blood cells (and other solids).
- a preferred thixotropic gel is a polyester based formulation, however any thixotropic gel can be used. Other nonlimiting examples are a mixture of silicon fluid and a hydrophobic powdered silica or a mixture of a hydrocarbon polymer and a powdered silica.
- Another preferred embodiment uses a thixotropic gel which is UV-curable in order to improve the strength of the barrier that is formed between the serum or plasma and the blood cells and other solids.
- Alternative types of separators include mechanical separators (e.g. elastomer barriers such as used in BD Barricor technology) and filter-based separators.
- the separator 31 is a thixotropic gel.
- the specimen container is centrifuged while oriented such that the closed end 33 of the cap 30 is further away from the axis of centrifugation 38 than the closed end 21 of the specimen tube 20 .
- blood cells 40 and any other solids that have a greater specific gravity than serum or plasma migrate to the reservoir 35 .
- FIG. 3 E shows the specimen container after it is centrifuged while oriented (in this case shown in an inverted orientation) so that the blood cells 40 migrate toward the closed end 33 of the cap 30 and into the reservoir 35 .
- the thixotropic gel separator 31 migrates above the blood cells 40 and forms a layer between the serum or plasma 42 and the blood cells 40 .
- the serum or plasma 42 migrates above the thixotropic gel separator 31 .
- the specimen container is orientated upright with the cap 30 above the specimen tube 20 such that gravity moves the serum or plasma 42 to the closed end 21 of the specimen tube 20 .
- the thixotropic gel separator 31 remains in the cap 30 of the specimen container and keeps the blood cells 40 trapped within the cap 30 .
- the cap 30 is removed from the specimen tube 20 .
- the blood cells 40 and thixotropic gel separator 31 are also removed because they are contained within in the reservoir 35 .
- the serum or plasma 42 is retained in the specimen tube 20 .
- a pipette 50 is inserted into the specimen tube 20 to extract the serum or plasma 42 . Because there are no blood cells and no separator in the specimen tube, the pipette is able to safely descend to the bottom of the specimen tube to extract the serum or plasma without risk of contacting any of the separator or blood cells.
- the pipette has extracted nearly all of the serum or plasma, while leaving a very small unusable dead volume.
- the conventional method of separating serum or plasma from whole blood leaves the blood cells and separator at the bottom of the specimen tube, which results in significantly higher dead volume because the pipette must keep a safe distance from the separator and blood cells to ensure there is no contact.
- the invention achieves having a significantly smaller dead volume, without incurring the added cost and risk of pouring the serum or plasma into a secondary specimen tube.
- cap design which includes a reservoir large enough to contain the blood cells from the specimen along with any other solids or separator to be removed.
- the advantage of containing and capturing blood cells, other solids and any separator within the cap is that when the cap is removed, the blood cells and any other solids or separator are removed with the cap, leaving only serum or plasma in the specimen tube to be pipetted.
- Another important element of the present invention is the technique of centrifuging the specimen container while oriented with the closed end of the cap further away from the axis of centrifugation than the closed end of the specimen tube. Centrifuging the specimen container with the closed end of the cap further way from the axis of centrifugation than the closed end of the specimen tube captures the blood cells, other solids and any separator in the reservoir in the cap. This enables the blood cells, other solids and any separator to be removed from the specimen when the cap is removed after centrifugation leaving just the serum or plasma in the specimen tube to be extracted for testing.
- FIGS. 4 A and 4 B depict a preferred embodiment of the present invention.
- FIG. 4 A depicts a sample container 50 with cap 51 secured to sample tube 52 .
- the cap 51 is secured by threaded connection 53 which secures to the internal threading of the sample tube 52 .
- the cap 51 is structured such that the reservoir 58 extends into the sample tube 52 .
- the sample container 50 contains a whole blood specimen 54 and a thixotropic gel 55 as a separator.
- FIG. 4 A depicts the sample container prior to centrifugation.
- FIG. 4 B depicts the sample container after centrifugation and shows the blood cells and other solids 56 separated from the serum or plasma 57 by the thixotropic gel 55 which acts as a separator.
- the sample container was centrifuged with the closed end 61 of the cap 51 further away from the axis of centrifugation 38 than the closed end 62 of the specimen tube 52 so that the components of greater specific gravity (i.e. blood cells and any other solids to be removed from the whole blood specimen) moved to the closed end 61 of the cap 51 .
- the thixotropic gel 55 separator which has a specific gravity intermediate to that of the blood cells and serum or plasma forms a layer between the blood cells and any other solids to be removed and the serum or plasma.
- the cap 51 can then be removed leaving only serum or plasma contained in the specimen tube.
- FIG. 1 A shows a cylindrical specimen tube as is typically used to collect blood specimens, however the specimen tube need not be cylindrical in shape.
- the invention could apply to specimen tubes with any shape desired.
- FIGS. 1 A-B , 2 , 3 A-I, and 4 A-B do not show the dimensions of the specimen container. Due to the applicability to small sample volumes, the size of the specimen container is preferably a micro-sample tube in the range of 0.1 mL to 2.0 mL. However, the invention is also applicable to larger conventional specimen tubes in the range of 2.0 mL to 10.0 mL or greater. The invention is not specific to any particular dimension of specimen container and can be applied to specimen tubes of any size.
- FIG. 1 A shows the use of a specimen tube having a round closed end. This is a preferred embodiment because this geometry minimizes the dead volume for a conventional pipette.
- the invention is not specific to any particular shape of specimen tube or specimen container and can be applied to specimen tubes or specimen containers of any shape.
- FIG. 3 B shows whole blood added to the specimen container while there is no cap on the specimen container.
- An alternate approach would be to add blood to the specimen container while the cap is attached.
- the closed end of the cap would contain a pierceable material such that a cannula can be inserted through the closed end of the cap to insert a blood specimen into the specimen container.
- the pierceable material would need to be such that it will reseal so that the specimen container does not leak the blood specimen contained within.
- cap surface 15 in FIG. 1 C or at least part thereof would be made up a material capable of being punctured with a needle. The material should be self-sealable such that after it is punctured with a needle, it will reseal so that the specimen does not leak out of the cap.
- This embodiment shall also embody any configuration wherein the closed end of the cap can be unsealed and resealed such that a cannula can insert blood through the closed end of the cap.
- the volume inside the specimen container is evacuated. This causes the blood specimen to be pulled into the specimen container by air pressure.
- the figures show the specimen container and cap having a screw-type connection in order to connect the cap to the top end of the specimen container.
- a screw-type connection is the preferred embodiment as it provides the most secure seal for centrifugation.
- the cap may be attached to the specimen container by a variety of methods. A few examples are provided but the invention should not be limited to the examples and should include any method for securing the cap to the specimen container.
- a stopper-type connection may be used, but extra sealing pressure may be required to be applied during centrifugation.
- a gasket between the tube and the cap may be useful in such an embodiment to improve the seal.
- the cap may be physically tethered to the specimen container so that they are always connected. Once the blood specimen is centrifuged to separate the serum or plasma from the blood cells and other solids, the cap should be able to be opened such that a pipette is able to be inserted into the specimen tube to pipette the serum or plasma or such that the serum or plasma can be poured out into a separate container such as a different specimen tube.
- An alternative embodiment is to integrate the cap into another device.
- a blood collection device with a threaded opening could act as the cap for a specimen tube.
- This blood collection device could have a dual function where it puts blood into the specimen tube and also acts as the cap.
- An alternative embodiment is to have a single device which functions as multiple caps.
- a single plastic device with multiple threaded openings could act as the cap for multiple specimen tubes, with each threaded opening having its own reservoir. What is essential is that each threaded opening, which acts as a cap, contains a reservoir large enough to hold the blood cells and any other solids or separator.
- An alternative embodiment is to have a single device which functions as multiple specimen tubes.
- a multi-well plate could act as multiple specimen tubes, with each well in the plate capable of being secured by a cap which contains a reservoir.
- FIGS. 3 A- 3 I, 4 A and 4 B include the use of a thixotropic gel as a separator gel. This design is preferred as the gel provides a reliable barrier to prevent the blood cells from remixing with the serum or plasma. An alternative is to not use any separator and rely on the centrifugation to pack the blood cells tightly enough in the reservoir such that they remain in the cap after centrifugation and when the cap is removed.
- FIG. 3 C shows the separator (e.g. a thixotropic gel) initially contained in the cap.
- This embodiment is preferred because it allows the specially designed cap to be used with commodity specimen tubes which do not contain a separator (e.g. a thixotropic gel).
- An alternative embodiment is to have the separator gel (e.g. a thixotropic gel) initially contained in the specimen tube.
- FIG. 3 E shows that the size of the reservoir has been designed such that the maximum volume it can hold is the volume of blood cells plus the volume of the separator gel. This minimizes the size of the cap while still ensuring that the blood cells and separator gel will be fully contained within the cap.
- the cap must be of sufficient size so that the reservoir has a volume sufficient to hold the volume of blood cells plus the volume of any other solids such as the separator.
- An alternative embodiment is to size the reservoir such that its volume is equal to or greater than the specimen tube's volume. This would ensure that during centrifugation there is no pressure on the seal between the specimen tube and the cap.
- a clot activator such as silica may be used.
- an anticoagulant such as lithium heparin or potassium EDTA can be used. This invention is not limited to the use of any particular additive.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Clinical Laboratory Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Thermal Sciences (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Centrifugal Separators (AREA)
Abstract
Description
- This application is a continuation of U.S. patent application Ser. No. 17/082,759, filed Oct. 28, 2020, which is a continuation of U.S. patent application Ser. No. 16/061,309, filed Jun. 11, 2018, (now U.S. Pat. No. 10,870,110) which is a U.S. National Phase application, filed under U.S.C. § 371(c), of International Application No. PCT/US2016/066236, filed Dec. 12, 2016, which claims priority of U.S. Provisional Appl. No. 62/266,433, filed Dec. 11, 2015, each of which i-s are incorporated by reference herein in their entireties.
- The present invention relates to a device and method for separating serum or plasma from whole blood.
- Many in vitro diagnostic testing systems and protocols require the use of blood specimens that are free of blood cells in order to perform a diagnostic test. These diagnostic tests either use serum or plasma which is separated from whole blood using techniques such as centrifugation or filtration.
- Serum can be separated from whole blood by first allowing the blood to clot and then centrifuging the specimen to move all the blood cells to the bottom of the specimen tube. Plasma can be separated from whole blood by first mixing the whole blood with an anticoagulant such as lithium heparin or potassium EDTA and then centrifuging the specimen to move all the blood cells to the bottom of the specimen tube.
- When using centrifugation to separate serum or plasma from whole blood, it is common to use a specimen tube that contains a thixotropic gel which has a greater specific gravity than serum or plasma, but lower specific gravity than blood cells. During centrifugation, this gel migrates above the blood cells while staying below the serum or plasma. The function of the gel is to provide a barrier between the serum or plasma and the blood cells so that the serum or plasma do not remix with the blood cells after centrifugation.
- One difficulty of using serum or plasma from centrifuged specimen tubes is that it is difficult to extract serum or plasma from the specimen tube without leaving a large dead volume. The term “dead volume” refers to the amount of unusable sample left in the specimen tube after the maximum amount of sample has been extracted. When using an automated or manual pipette to extract sample from a centrifuged specimen tube, there is a risk that the pipette will make contact with either the blood cells or the gel separator. If this occurs, the sample may be disturbed remixing the blood cells and serum or plasma, the pipette may get clogged, or the pipette may not extract pure serum or plasma. To avoid these risks, the pipette must be kept a safe depth above the blood cells or gel separator to ensure it does not make contact with the blood cells or the gel separator. For an automated pipette, this means that the pipette depth is controlled such that the pipette tip keeps a safe distance from the blood cells or the gel separator. For a manual pipette, this means that the user exercises caution to keep the pipette a safe distance from the blood cells or the gel separator. The serum or plasma which is above the blood cells or the gel separator yet below the pipette tip is unusable as it won't be extracted in the pipette.
- Leaving a large dead volume may not be problematic when the amount of serum or plasma available is significantly greater than the amount required by the diagnostic tests. However, as the amount of serum or plasma required by the diagnostic tests approaches the amount of serum or plasma available, dead volume becomes a greater concern. This is particularly important when using small volume sample collection technologies or in pediatric samples where the amount of blood able to be drawn is more limited.
- In cases where it is not possible to collect larger amounts of a blood specimen, one common technique to reduce the dead volume left when pipetting from a centrifuged specimen container is to pour the serum or plasma out of the centrifuged specimen tube, into a secondary specimen tube. Because the secondary specimen tube does not contain blood cells or gel separator, a pipette can safely dive to the bottom of the specimen tube and the serum or plasma can be extracted with a low dead volume. While this technique results in a low dead volume, there are several significant disadvantages. This technique consumes an additional specimen container, which results in added material costs. The step of pouring serum or plasma increases labor costs and introduces risk of human error. There is also a risk of specimen mix-up if the new specimen tube is not properly labeled.
- In order to, inter alia, make a blood draw less invasive and decrease the costs of the running diagnostic tests, many companies are currently developing specimen collection and processing techniques based on smaller sample volumes than are collected by most labs today. In order to effectively run diagnostic tests using serum or plasma collected in small volumes, approaches are needed to minimize the loss of serum or plasma due to dead volume. This invention enables diagnostic laboratories to effectively run diagnostic tests using smaller blood specimens or run more tests with the same volume of blood specimen.
- Provided herein is a device for separating serum or plasma from blood cells in a whole blood specimen. The present invention uses a cap with a reservoir, such that blood cells are packed into the cap when a specimen tube is centrifuged with the capped end away from the axis of centrifugation. When the cap is removed, the blood cells are also removed, so that the serum or plasma is left in the specimen tube where it can be readily extracted by a pipette which is able to reach all the way to the bottom of the specimen tube minimizing the dead volume.
-
FIG. 1A-1C show a cross-sectional view of the specimen tube and cap according to the invention. -
FIG. 2 shows a cross-sectional view of the specimen container according to the invention. -
FIG. 3A-3I depicts the methodology for separating serum or plasma from blood cells according to the invention. -
FIGS. 4A and 4B depicts a preferred embodiment of the specimen container according to the present invention. - Various terms relating to aspects of the description are used throughout the specification and claims. Such terms are to be given their ordinary meaning in the art unless otherwise indicated. Other specifically defined terms are to be construed in a manner consistent with the definitions provided herein.
- As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise.
- The term “about” as used herein when referring to a measurable value such as an amount and the like, is meant to encompass variations of up to ±30% from the specified value, as such variations are appropriate to perform the disclosed methods. Unless otherwise indicated, all numbers expressing quantities of properties such as volume and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention.
-
FIG. 1 depicts a specimen tube, often called a test tube, in which a blood specimen is collected according to the present invention. Thespecimen tube 1 has a closedend 3,open end 4 and lateral wall(s) 2. Theopen end 4 enables a liquid specimen to be inserted into thespecimen tube 1. The closedend 3 is shown in a preferred embodiment with a round bottom. Specimen tubes having a round or conical bottom are preferred as they minimize dead volume when pipetting from the bottom of the specimen tube. While specimen tubes with round or conical bottoms are preferred, the bottom of the specimen tube can be any shape. -
FIG. 1B depicts acap 10 to secure onto a specimen tube such as that shown inFIG. 1A . Thecap 10 has anopen end 12, aclosed end 13 and alateral wall 14. Theclosed end 13 is closed by asurface 15. Thecap 10 has areservoir 16 which is formed when thecap 10 andspecimen tube 1 are secured to formed a specimen container comprising the specimen tube and the cap, as shown inFIG. 2 , where the volume of thereservoir 16 is defined as the maximum amount of liquid that can be contained in thecap 10 without coming into contact with thespecimen tube 1. In order to remove the blood cells or other solids in the blood from the serum or plasma in accordance with the present invention, the volume of thereservoir 16 should be equal to or greater than the volume of the blood cells and other solids to be separated from the serum or plasma in the blood specimen. This will enable to the blood cells and other solids to be removed when thecap 10 is removed from thespecimen tube 1. In another embodiment, the cap is sized so that volume of the reservoir is equal to or greater than the volume of whole blood in the specimen tube. This design will decrease or eliminate the pressure on the seal between the specimen tube and the cap during centrifugation. - The
specimen tube 1 and cap 10 have a mechanism by which theopen end 4 of thespecimen tube 1 may be secured with theopen end 12 of thecap 10 to form a specimen container, such that the blood specimen is contained within thespecimen tube 1 and thecap 10. The mechanism for securing thespecimen tube 1 and thecap 10 can be any mechanism desired as long as the specimen tube and cap are secured to create a leak free fitting. It is important that the contents of the specimen container do not leak when the specimen container is oriented in any direction. Such mechanisms for securing the specimen tube to the cap can include an engineered fit or interference fit. A preferred engineered fit is a threadedconnection 7 where the specimen tube and the cap both have screw threads that work to connect the specimen tube and the cap together as shown in the figures. Any other engineered fit can be used that creates a leak free locking mechanism. Alternatively, an interference fit can be used such that the specimen tube and the cap are secured by friction after the open end of the specimen tube and open end of the cap are pushed together. For example, the cap can be a plug that is secured onto the open end of the specimen tube. Optionally, the joint between the specimen tube and the cap can be sealed using a gasket. The mechanism to secure the specimen tube and cap together is not limited to any particular mechanism and shall include any mechanism whereby the specimen tube and cap can be secured together to create a leak free specimen container. -
FIGS. 3A-3I depict an embodiment of the inventive method to separate serum or plasma from a blood specimen and works as follows: - In
FIG. 3A anempty specimen tube 20 having aclosed end 21,open end 22 and lateral wall(s) 23 is shown.FIG. 3B shows thespecimen tube 20 filled with awhole blood specimen 24 which was inserted throughopen end 22. InFIG. 3C , acap 30, such as that shown inFIG. 1B , is secured to theopen end 22 of thespecimen tube 20. A specimen tube that is enclosed with a cap is referred to herein as a specimen container. The cap has anopen end 32, aclosed end 33 and alateral wall 34. Thecap 30 contains aseparator 31. - For purposes of this invention, a separator is a device that is put into a specimen tube (or alternatively, into a cap) in order to ensure that after the specimen tube is centrifuged, there is a physical layer separating the serum or plasma from the blood cells and other solids. The separator is chosen such that it has a higher specific gravity than serum and/or plasma and lower specific gravity than blood cells and other solids in the blood to be removed. During centrifugation, the serum or plasma is separated from blood cells and any other solids in the blood by migrating closer to the axis of centrifugation because it has lower specific gravity. The blood cells (and other solids) migrate further away from the axis of centrifugation because they have higher specific gravity. The separator migrates to a level between the serum or plasma and the blood cells (and other solids), because it has intermediate specific gravity. The primary function of the separator is to maintain the purity of the serum or plasma by 1) preventing the serum or plasma from remixing with the blood cells, and 2) preventing the serum or plasma from becoming contaminated by the blood cells as they degrade.
- A preferred separator is a thixotropic gel. This is a hydrophobic gel which is initially solid, but becomes liquefied during centrifugation so that it can migrate to form a layer between the serum or plasma and the blood cells (and other solids). A preferred thixotropic gel is a polyester based formulation, however any thixotropic gel can be used. Other nonlimiting examples are a mixture of silicon fluid and a hydrophobic powdered silica or a mixture of a hydrocarbon polymer and a powdered silica. Another preferred embodiment uses a thixotropic gel which is UV-curable in order to improve the strength of the barrier that is formed between the serum or plasma and the blood cells and other solids. Alternative types of separators include mechanical separators (e.g. elastomer barriers such as used in BD Barricor technology) and filter-based separators.
- In the preferred embodiment shown in
FIG. 3A-3I , theseparator 31 is a thixotropic gel. InFIG. 3D , the specimen container is centrifuged while oriented such that theclosed end 33 of thecap 30 is further away from the axis ofcentrifugation 38 than theclosed end 21 of thespecimen tube 20. During centrifugation,blood cells 40 and any other solids that have a greater specific gravity than serum or plasma migrate to thereservoir 35.FIG. 3E shows the specimen container after it is centrifuged while oriented (in this case shown in an inverted orientation) so that theblood cells 40 migrate toward theclosed end 33 of thecap 30 and into thereservoir 35. Due to the difference in specific gravity, thethixotropic gel separator 31 migrates above theblood cells 40 and forms a layer between the serum orplasma 42 and theblood cells 40. The serum orplasma 42 migrates above thethixotropic gel separator 31. In Figure F, the specimen container is orientated upright with thecap 30 above thespecimen tube 20 such that gravity moves the serum orplasma 42 to theclosed end 21 of thespecimen tube 20. Thethixotropic gel separator 31 remains in thecap 30 of the specimen container and keeps theblood cells 40 trapped within thecap 30. InFIG. 3G , thecap 30 is removed from thespecimen tube 20. Theblood cells 40 andthixotropic gel separator 31 are also removed because they are contained within in thereservoir 35. The serum orplasma 42 is retained in thespecimen tube 20. InFIG. 3H , apipette 50 is inserted into thespecimen tube 20 to extract the serum orplasma 42. Because there are no blood cells and no separator in the specimen tube, the pipette is able to safely descend to the bottom of the specimen tube to extract the serum or plasma without risk of contacting any of the separator or blood cells. InFIG. 3I , the pipette has extracted nearly all of the serum or plasma, while leaving a very small unusable dead volume. The conventional method of separating serum or plasma from whole blood leaves the blood cells and separator at the bottom of the specimen tube, which results in significantly higher dead volume because the pipette must keep a safe distance from the separator and blood cells to ensure there is no contact. The invention achieves having a significantly smaller dead volume, without incurring the added cost and risk of pouring the serum or plasma into a secondary specimen tube. - An important element of the present invention is the cap design, which includes a reservoir large enough to contain the blood cells from the specimen along with any other solids or separator to be removed. The advantage of containing and capturing blood cells, other solids and any separator within the cap is that when the cap is removed, the blood cells and any other solids or separator are removed with the cap, leaving only serum or plasma in the specimen tube to be pipetted.
- Another important element of the present invention is the technique of centrifuging the specimen container while oriented with the closed end of the cap further away from the axis of centrifugation than the closed end of the specimen tube. Centrifuging the specimen container with the closed end of the cap further way from the axis of centrifugation than the closed end of the specimen tube captures the blood cells, other solids and any separator in the reservoir in the cap. This enables the blood cells, other solids and any separator to be removed from the specimen when the cap is removed after centrifugation leaving just the serum or plasma in the specimen tube to be extracted for testing.
-
FIGS. 4A and 4B depict a preferred embodiment of the present invention.FIG. 4A depicts asample container 50 withcap 51 secured to sampletube 52. Thecap 51 is secured by threadedconnection 53 which secures to the internal threading of thesample tube 52. In this embodiment, thecap 51 is structured such that thereservoir 58 extends into thesample tube 52. Thesample container 50 contains awhole blood specimen 54 and athixotropic gel 55 as a separator.FIG. 4A depicts the sample container prior to centrifugation.FIG. 4B depicts the sample container after centrifugation and shows the blood cells andother solids 56 separated from the serum orplasma 57 by thethixotropic gel 55 which acts as a separator. The sample container was centrifuged with theclosed end 61 of thecap 51 further away from the axis ofcentrifugation 38 than theclosed end 62 of thespecimen tube 52 so that the components of greater specific gravity (i.e. blood cells and any other solids to be removed from the whole blood specimen) moved to theclosed end 61 of thecap 51. Thethixotropic gel 55 separator which has a specific gravity intermediate to that of the blood cells and serum or plasma forms a layer between the blood cells and any other solids to be removed and the serum or plasma. Thecap 51 can then be removed leaving only serum or plasma contained in the specimen tube. - While particular embodiments of the invention have been described and illustrated, it is not intended that the invention be limited thereto. It is intended that the invention be as broad in scope as the art will allow and that the disclosure herein be interpreted likewise.
-
FIG. 1A shows a cylindrical specimen tube as is typically used to collect blood specimens, however the specimen tube need not be cylindrical in shape. The invention could apply to specimen tubes with any shape desired. -
FIGS. 1A-B , 2, 3A-I, and 4A-B do not show the dimensions of the specimen container. Due to the applicability to small sample volumes, the size of the specimen container is preferably a micro-sample tube in the range of 0.1 mL to 2.0 mL. However, the invention is also applicable to larger conventional specimen tubes in the range of 2.0 mL to 10.0 mL or greater. The invention is not specific to any particular dimension of specimen container and can be applied to specimen tubes of any size. -
FIG. 1A shows the use of a specimen tube having a round closed end. This is a preferred embodiment because this geometry minimizes the dead volume for a conventional pipette. The invention is not specific to any particular shape of specimen tube or specimen container and can be applied to specimen tubes or specimen containers of any shape. -
FIG. 3B shows whole blood added to the specimen container while there is no cap on the specimen container. An alternate approach would be to add blood to the specimen container while the cap is attached. In this embodiment, the closed end of the cap would contain a pierceable material such that a cannula can be inserted through the closed end of the cap to insert a blood specimen into the specimen container. The pierceable material would need to be such that it will reseal so that the specimen container does not leak the blood specimen contained within. This is commonly done today using evacuated specimen tubes that have a cap with a septum that can be pierced with a needle. In such an embodiment,cap surface 15 inFIG. 1C or at least part thereof would be made up a material capable of being punctured with a needle. The material should be self-sealable such that after it is punctured with a needle, it will reseal so that the specimen does not leak out of the cap. - This embodiment shall also embody any configuration wherein the closed end of the cap can be unsealed and resealed such that a cannula can insert blood through the closed end of the cap.
- In another preferred embodiment, the volume inside the specimen container is evacuated. This causes the blood specimen to be pulled into the specimen container by air pressure.
- The figures show the specimen container and cap having a screw-type connection in order to connect the cap to the top end of the specimen container. A screw-type connection is the preferred embodiment as it provides the most secure seal for centrifugation. However, the cap may be attached to the specimen container by a variety of methods. A few examples are provided but the invention should not be limited to the examples and should include any method for securing the cap to the specimen container. A stopper-type connection may be used, but extra sealing pressure may be required to be applied during centrifugation. A gasket between the tube and the cap may be useful in such an embodiment to improve the seal.
- The preferred embodiment described and illustrated show the specimen tube and cap as standalone components. In another embodiment, the cap may be physically tethered to the specimen container so that they are always connected. Once the blood specimen is centrifuged to separate the serum or plasma from the blood cells and other solids, the cap should be able to be opened such that a pipette is able to be inserted into the specimen tube to pipette the serum or plasma or such that the serum or plasma can be poured out into a separate container such as a different specimen tube.
- An alternative embodiment is to integrate the cap into another device. For example, a blood collection device with a threaded opening could act as the cap for a specimen tube. This blood collection device could have a dual function where it puts blood into the specimen tube and also acts as the cap.
- An alternative embodiment is to have a single device which functions as multiple caps. For example, a single plastic device with multiple threaded openings could act as the cap for multiple specimen tubes, with each threaded opening having its own reservoir. What is essential is that each threaded opening, which acts as a cap, contains a reservoir large enough to hold the blood cells and any other solids or separator.
- An alternative embodiment is to have a single device which functions as multiple specimen tubes. For example, a multi-well plate could act as multiple specimen tubes, with each well in the plate capable of being secured by a cap which contains a reservoir.
- The preferred embodiments described and illustrated in
FIGS. 3A-3I, 4A and 4B include the use of a thixotropic gel as a separator gel. This design is preferred as the gel provides a reliable barrier to prevent the blood cells from remixing with the serum or plasma. An alternative is to not use any separator and rely on the centrifugation to pack the blood cells tightly enough in the reservoir such that they remain in the cap after centrifugation and when the cap is removed. -
FIG. 3C shows the separator (e.g. a thixotropic gel) initially contained in the cap. This embodiment is preferred because it allows the specially designed cap to be used with commodity specimen tubes which do not contain a separator (e.g. a thixotropic gel). An alternative embodiment is to have the separator gel (e.g. a thixotropic gel) initially contained in the specimen tube. -
FIG. 3E shows that the size of the reservoir has been designed such that the maximum volume it can hold is the volume of blood cells plus the volume of the separator gel. This minimizes the size of the cap while still ensuring that the blood cells and separator gel will be fully contained within the cap. The cap must be of sufficient size so that the reservoir has a volume sufficient to hold the volume of blood cells plus the volume of any other solids such as the separator. An alternative embodiment is to size the reservoir such that its volume is equal to or greater than the specimen tube's volume. This would ensure that during centrifugation there is no pressure on the seal between the specimen tube and the cap. - The preferred embodiment described and illustrated describe the separation of serum or plasma from whole blood. This implies the possible use of chemical additives to the whole blood. For instance, to separate serum, a clot activator such as silica may be used. To separate plasma, an anticoagulant such as lithium heparin or potassium EDTA can be used. This invention is not limited to the use of any particular additive.
Claims (21)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18/323,830 US20240042427A1 (en) | 2015-12-11 | 2023-05-25 | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562266433P | 2015-12-11 | 2015-12-11 | |
PCT/US2016/066236 WO2017100798A1 (en) | 2015-12-11 | 2016-12-12 | Specimen container and method for separating serum or plasma from whole blood |
US201816061309A | 2018-06-11 | 2018-06-11 | |
US17/082,759 US11697114B2 (en) | 2015-12-11 | 2020-10-28 | Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells |
US18/323,830 US20240042427A1 (en) | 2015-12-11 | 2023-05-25 | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US17/082,759 Continuation US11697114B2 (en) | 2015-12-11 | 2020-10-28 | Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells |
Publications (1)
Publication Number | Publication Date |
---|---|
US20240042427A1 true US20240042427A1 (en) | 2024-02-08 |
Family
ID=59014350
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/061,309 Active 2037-06-26 US10870110B2 (en) | 2015-12-11 | 2016-12-12 | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
US17/082,759 Active US11697114B2 (en) | 2015-12-11 | 2020-10-28 | Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells |
US18/323,830 Pending US20240042427A1 (en) | 2015-12-11 | 2023-05-25 | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/061,309 Active 2037-06-26 US10870110B2 (en) | 2015-12-11 | 2016-12-12 | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith |
US17/082,759 Active US11697114B2 (en) | 2015-12-11 | 2020-10-28 | Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells |
Country Status (5)
Country | Link |
---|---|
US (3) | US10870110B2 (en) |
EP (2) | EP3386391B1 (en) |
CN (2) | CN113751095B (en) |
ES (1) | ES2846863T3 (en) |
WO (1) | WO2017100798A1 (en) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2868996A1 (en) | 2012-04-02 | 2013-10-10 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
CN113751095B (en) | 2015-12-11 | 2024-01-09 | 巴布森诊断公司 | Sample container and method for separating serum or plasma from whole blood |
EP3659065A4 (en) * | 2017-07-28 | 2020-08-19 | Siemens Healthcare Diagnostics Inc. | Deep learning volume quantifying methods and apparatus |
US20220403001A1 (en) | 2018-06-12 | 2022-12-22 | Obsidian Therapeutics, Inc. | Pde5 derived regulatory constructs and methods of use in immunotherapy |
WO2020013981A1 (en) | 2018-07-09 | 2020-01-16 | Hanuman Pelican, Inc. | Apparatus and methods for processing blood |
JP2021531842A (en) | 2018-07-09 | 2021-11-25 | ハヌマン ペリカン,インコーポレイテッド | Devices and methods for separating blood components |
US20220088589A1 (en) | 2019-01-21 | 2022-03-24 | Eclipse Medcorp, Llc | Methods, Systems and Apparatus for Separating Components of a Biological Sample |
EP3890799A4 (en) | 2019-02-06 | 2022-10-26 | Hanuman Pelican, Inc. | Apparatus and methods for concentrating platelet-rich plasma |
CN111743579B (en) * | 2020-07-07 | 2023-05-30 | 德阳市人民医院 | Infectious department protection type pathogen sample collection device |
CN113005177B (en) * | 2021-03-24 | 2022-08-30 | 杭州倍强医药科技有限公司 | Blood sample obtaining method, blood sample and application |
WO2023043760A1 (en) * | 2021-09-14 | 2023-03-23 | Becton, Dickinson And Company | Rigid separation barrier for a specimen collection container |
CA3232433A1 (en) * | 2021-09-21 | 2023-03-30 | Arun U. Nair | Dual chamber specimen collection container assembly |
WO2023196922A1 (en) | 2022-04-06 | 2023-10-12 | Babson Diagnostics, Inc. | Automated centrifuge loader |
CN114797586A (en) * | 2022-05-24 | 2022-07-29 | 宁波市第一医院 | Automatic urine sediment sheet-making workstation |
US11957465B2 (en) | 2022-08-23 | 2024-04-16 | Reddrop Dx, Inc. | Accelerated ergonomic collection of capillary blood |
Family Cites Families (254)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1892884A (en) * | 1928-06-19 | 1933-01-03 | Frank A Grauman | Sediment collecting stopper |
US2110237A (en) * | 1936-03-06 | 1938-03-08 | Swift & Co | Sediment tester |
US2240101A (en) * | 1938-05-10 | 1941-04-29 | Smith Fred | Closure for test tubes or the like |
US2722257A (en) * | 1953-02-12 | 1955-11-01 | Compule Corp | Sampling tube equipment |
US2775350A (en) * | 1953-12-28 | 1956-12-25 | Hugh A Jones | Filters |
US2912895A (en) * | 1954-03-18 | 1959-11-17 | Hamilton Robert Houston | Spectrophotometry process |
US2896502A (en) * | 1956-05-07 | 1959-07-28 | Thornhill Craver Company Inc | Colorimeter apparatus |
US3081029A (en) * | 1959-06-19 | 1963-03-12 | Copolymer Rubber & Chem Corp | Improved centrifuge tube |
NL109202C (en) * | 1959-11-20 | |||
US3300051A (en) * | 1963-09-26 | 1967-01-24 | Internat Equipment Co | Filter tube for use in a centrifuge |
GB1088421A (en) * | 1964-01-07 | 1967-10-25 | Harshaw Chemicals Ltd | A method and apparatus for depositing a coating on the internal walls of capillary or smallbore tubes |
GB1112715A (en) * | 1965-07-16 | 1968-05-08 | Lancelot Richard Rowett | Disposable urine specimen tube and cap therefor |
US3326400A (en) * | 1965-10-23 | 1967-06-20 | Oreal | Two compartment container |
US3419179A (en) * | 1967-06-07 | 1968-12-31 | Brunswick Corp | Captive cap specimen vial |
US3478889A (en) * | 1967-08-31 | 1969-11-18 | Julius H Fessler | Filter apparatus |
US3539300A (en) * | 1967-10-23 | 1970-11-10 | Schering Corp | Body fluid collector and separator having improved flow rate |
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3419178A (en) * | 1968-01-04 | 1968-12-31 | Wendell H. Swartz | Cigarette server |
CH500707A (en) | 1968-07-26 | 1970-12-31 | Micromedic Systems Inc | Device for performing percutaneous and digital blood sampling |
US3733179A (en) * | 1968-08-29 | 1973-05-15 | Minnesota Mining & Mfg | Method and apparatus for the quantitative determination of blood chemicals in blood derivatives |
US3615222A (en) * | 1968-09-04 | 1971-10-26 | New England Nuclear Corp | Method and apparatus for measuring the amount of a component in a biological fluid |
US3525254A (en) * | 1969-02-19 | 1970-08-25 | Jesus R Milanes | Device and method for testing blood coagulation factors |
US3654925A (en) * | 1969-09-23 | 1972-04-11 | Becton Dickinson Co | Plasma separator system |
US3684455A (en) * | 1969-12-19 | 1972-08-15 | Mallinckrodt Chemical Works | Apparatus for mixing liquids |
US3611403A (en) | 1970-04-13 | 1971-10-05 | Gilford Instr Labor Inc | Test sample container identification method and apparatus |
US3721528A (en) * | 1970-06-04 | 1973-03-20 | L Mead | Method and apparatus for measuring the amount of a component in a biological fluid |
US3750645A (en) * | 1970-10-20 | 1973-08-07 | Becton Dickinson Co | Method of collecting blood and separating cellular components thereof |
US3743482A (en) * | 1970-12-30 | 1973-07-03 | Nuclear Med Lab | Method and apparatus for determining thyroid function |
US3706305A (en) * | 1971-03-03 | 1972-12-19 | Harold J Berger | Combination blood sampling vacuum syringe centrifuge container and specimen cup |
US3706306A (en) * | 1971-03-03 | 1972-12-19 | Harold J Berger | Combination blood sampling vacuum syringe centrifuge container and specimen cup |
US3701434A (en) * | 1971-03-15 | 1972-10-31 | Hugh C Moore | Test tube system for separating blood into serum and red cells |
US3814248A (en) * | 1971-09-07 | 1974-06-04 | Corning Glass Works | Method and apparatus for fluid collection and/or partitioning |
DE2153214A1 (en) | 1971-10-26 | 1973-05-03 | Philips Patentverwaltung | DEVICE TO ACCEPT AN IDENTIFICATION CARRIER HOLDER |
US3849072A (en) * | 1972-04-25 | 1974-11-19 | Becton Dickinson Co | Plasma separator |
US3761408A (en) * | 1972-05-08 | 1973-09-25 | Yoon Lee Jae | Method and apparatus for separating blood constituent components |
US3780935A (en) * | 1972-07-10 | 1973-12-25 | Lukacs & Jacoby Ass | Serum separating method |
US3852194A (en) * | 1972-12-11 | 1974-12-03 | Corning Glass Works | Apparatus and method for fluid collection and partitioning |
US3786985A (en) * | 1973-01-05 | 1974-01-22 | Hoffmann La Roche | Blood collection container |
US3942717A (en) * | 1973-02-09 | 1976-03-09 | Robison William O | Specimen container |
US3928139A (en) * | 1973-02-12 | 1975-12-23 | Wadley Res Inst & Blood Bank | Detection of microbial pathogens |
US3926521A (en) * | 1973-02-21 | 1975-12-16 | Byron E Ginzel | Blood collecting and processing means |
US3879295A (en) * | 1973-08-17 | 1975-04-22 | Eastman Kodak Co | Vacutainer with positive separation barrier |
US3862042A (en) * | 1974-02-27 | 1975-01-21 | Becton Dickinson Co | Serum/plasma separator - piston with red-cell trapping surfaces |
US3931010A (en) * | 1974-02-27 | 1976-01-06 | Becton, Dickinson And Company | Serum/plasma separators with centrifugal valves |
US3920549A (en) * | 1974-03-18 | 1975-11-18 | Corning Glass Works | Method and apparatus for multiphase fluid collection and separation |
US3958944A (en) * | 1974-07-15 | 1976-05-25 | Wong Johnson N S | Vial assembly |
US3929646A (en) * | 1974-07-22 | 1975-12-30 | Technicon Instr | Serum separator and fibrin filter |
US3939822A (en) * | 1974-08-14 | 1976-02-24 | Jack Markowitz | Disposable blood collection and filtering device |
US3985649A (en) * | 1974-11-25 | 1976-10-12 | Eddelman Roy T | Ferromagnetic separation process and material |
CH587486A5 (en) * | 1974-11-29 | 1977-05-13 | Hoffmann La Roche | |
US4012325A (en) * | 1975-01-08 | 1977-03-15 | Eastman Kodak Company | Biological fluid dispenser and separator |
US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
US4052320A (en) * | 1975-08-29 | 1977-10-04 | Eastman Kodak Company | Telescoping serum separator and dispenser |
GB1562900A (en) * | 1975-09-24 | 1980-03-19 | Aes Scient Ltd | Preparation of blood plasma and serum samples |
USD246800S (en) * | 1975-10-20 | 1977-12-27 | Wong Johnson N S | Vial |
US4083788A (en) * | 1975-11-19 | 1978-04-11 | Ferrara Louis T | Blood serum-isolation device |
US4180465A (en) * | 1975-12-19 | 1979-12-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase separation means |
US4055501A (en) * | 1976-01-16 | 1977-10-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase partitioning means |
US4088582A (en) * | 1976-01-16 | 1978-05-09 | Sherwood Medical Industries Inc. | Blood phase separation means |
US4050451A (en) * | 1976-08-13 | 1977-09-27 | Eastman Kodak Company | Blood collection and separation device |
CA1077297A (en) * | 1976-04-07 | 1980-05-13 | Richard L. Columbus | Capillary collection and dispensing device for non-pressurized liquid |
US4081356A (en) * | 1976-09-24 | 1978-03-28 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Fecalator, an apparatus and method for concentration of parasite eggs and larvae |
US4046699A (en) * | 1976-11-01 | 1977-09-06 | Corning Glass Works | Access device for centrifugal separation assemblies |
US4131512A (en) * | 1976-11-05 | 1978-12-26 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Method for detecting microbial pathogens employing a cushioning agent |
US4132225A (en) * | 1976-11-18 | 1979-01-02 | Hynson, Westcott & Dunning, Inc. | Micro blood collector |
AT381466B (en) * | 1977-03-16 | 1986-10-27 | Ballies Uwe | SEPARATING TUBES FOR CENTRIFUGAL SEPARATION |
US4131549A (en) * | 1977-05-16 | 1978-12-26 | Ferrara Louis T | Serum separation device |
US4169060A (en) * | 1977-10-25 | 1979-09-25 | Eastman Kodak Company | Blood-collecting and serum-dispensing device |
US4164449A (en) * | 1977-11-03 | 1979-08-14 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Surface separation technique for the detection of microbial pathogens |
US4147628A (en) * | 1978-01-23 | 1979-04-03 | Becton, Dickinson And Company | Blood partitioning method |
US4235725A (en) * | 1978-08-16 | 1980-11-25 | Owens-Illinois, Inc. | Sterile blood-collecting and separating device |
US4257886A (en) * | 1979-01-18 | 1981-03-24 | Becton, Dickinson And Company | Apparatus for the separation of blood components |
US4227620A (en) * | 1979-02-28 | 1980-10-14 | Becton, Dickinson And Company | Specimen collecting tube |
JPS5917386B2 (en) * | 1979-03-23 | 1984-04-20 | テルモ株式会社 | Blood separation method and device |
US4308232A (en) * | 1979-07-09 | 1981-12-29 | Sherwood Medical Industries Inc. | Anticoagulant stopper coating |
US4295974A (en) * | 1980-05-05 | 1981-10-20 | Sherwood Medical Industries Inc. | Blood sample collection and phase separation device |
EP0039898B1 (en) * | 1980-05-08 | 1984-08-22 | Terumo Corporation | Apparatus for separating blood |
US4369117A (en) * | 1980-05-12 | 1983-01-18 | American Hospital Supply Corporation | Serum separating method and apparatus |
US4358425A (en) | 1981-02-17 | 1982-11-09 | Beckman Instruments, Inc. | Penetrable centrifuge tube |
US4417981A (en) * | 1981-05-04 | 1983-11-29 | Becton, Dickinson And Company | Blood phase separator device |
US4443408A (en) | 1981-07-09 | 1984-04-17 | International Technidyne, Inc. | Apparatus for analyzing the influence of additive reagents upon the coagulation of blood |
US4671939A (en) * | 1981-07-09 | 1987-06-09 | International Technidyne Corp. | Apparatus for analyzing the influence of additive reagents upon the coagulation of blood and related methods |
US4425235A (en) * | 1982-03-22 | 1984-01-10 | Sherwood Medical Company | Blood collection device with phase partitioning means |
US4513522A (en) * | 1982-09-16 | 1985-04-30 | Selenke William M | Label with particular application to laboratory specimen container identification |
DE3316335A1 (en) * | 1983-05-04 | 1984-11-08 | Fa. Andreas Hettich, 7200 Tuttlingen | METHOD FOR DETERMINING PARTS OF A SAMPLING LIQUID ON A SLIDE AND DEVICE FOR EXERCISING THE METHOD |
IT1199121B (en) * | 1984-05-09 | 1988-12-30 | Paolo Giuseppe Campolo | DEVICE FOR A FAST AND AUTOMATIC REMOVAL OF A LIQUID PHASE FROM A SOLID PHASE INSIDE A CONTAINER, IN PARTICULAR WAY OF A TEST TUBE |
US4678559A (en) * | 1984-07-23 | 1987-07-07 | Andreas Szabados | Test specimen container for pasty specimen material |
EP0231191A4 (en) * | 1984-11-07 | 1988-04-27 | Ross Thomas Starr | Measurement of total iron binding capacity. |
FR2582013A1 (en) * | 1985-05-15 | 1986-11-21 | Guillon Leone | Process for removing the crust formed during the secondary fermentation of a wine treated according to the champagne method and other methods |
US4762798A (en) * | 1985-12-31 | 1988-08-09 | Marshall Diagnostics, Inc. | Device and method for determining a characteristic of a fluid sample |
US4755356A (en) * | 1986-01-23 | 1988-07-05 | Robbins Scientific Corporation | Locking microcentrifuge tube |
US4775626A (en) * | 1986-05-23 | 1988-10-04 | Syntex (U.S.A.) Inc. | Method and compositions for protecting anerobic microorganisms |
US4811866A (en) * | 1987-01-02 | 1989-03-14 | Helena Laboratories Corporation | Method and apparatus for dispensing liquids |
US5019243A (en) * | 1987-04-03 | 1991-05-28 | Mcewen James A | Apparatus for collecting blood |
US5030341A (en) * | 1987-04-03 | 1991-07-09 | Andronic Technologies, Inc. | Apparatus for separating phases of blood |
DE3722563A1 (en) * | 1987-07-08 | 1989-01-19 | Andreas Szabados | FILTRATION UNIT WITH PRESSURE COMPENSATION |
US4832678A (en) * | 1987-12-03 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Adapter for a centrifuge tube and a removal tool therefor |
US4805772A (en) | 1988-02-26 | 1989-02-21 | Eastman Kodak Company | Adaptors for use with various containers bearing bar code labeling |
DK162628C (en) * | 1988-05-02 | 1992-04-13 | Bjoern Nielsen | COLLECTION, CANNEL, HOLDER AND VACUUM GLASS |
US4957707A (en) * | 1988-08-31 | 1990-09-18 | The Dow Chemical Company | Thermal hazard evaluation |
JPH03181852A (en) | 1989-12-12 | 1991-08-07 | Hirokazu Yamamoto | Method and container for collecting blood clot from test tube after separation of serum by centrifugal method from blood specimen collected using serum separating agent-containing test tube |
US5151184A (en) * | 1990-11-14 | 1992-09-29 | Biomedical Devices Company | Fluid collecting and dispensing system |
IT1246994B (en) * | 1991-01-10 | 1994-12-12 | Diesse Diagnostica | PERFORABLE CAP FOR BLOOD COLLECTION WITH DOUBLE NEEDLE DEVICES IN VACUUM TUBES |
US5316146A (en) * | 1991-03-06 | 1994-05-31 | Ulster Scientific, Inc. | Vial transporter |
US5236604A (en) * | 1991-05-29 | 1993-08-17 | Sherwood Medical Company | Serum separation blood collection tube and the method of using thereof |
US5275731A (en) * | 1991-06-28 | 1994-01-04 | Jahn Karl H | Apparatus for rapidly separating blood into filtered fractions |
JPH0526883A (en) | 1991-07-19 | 1993-02-02 | Nittec Co Ltd | Automatic analyzer |
US5501841A (en) | 1991-11-14 | 1996-03-26 | Artchem, Inc. | Connection-type treatment system for micro solution and method of treatment |
CA2058917A1 (en) * | 1992-01-07 | 1993-07-08 | Alan Richard Graham | Captrap |
US5271852A (en) * | 1992-05-01 | 1993-12-21 | E. I. Du Pont De Nemours And Company | Centrifugal methods using a phase-separation tube |
GB9220597D0 (en) * | 1992-09-30 | 1992-11-11 | Boyde Thomas | Multilocular sample containers for blood or other fluids |
US5290703A (en) * | 1992-12-14 | 1994-03-01 | Miles, Inc. | Method for the separation of high density lipoprotein from blood samples |
US5352410A (en) | 1993-06-03 | 1994-10-04 | Hansen Warren D | Fluid specimen collection and testing apparatus |
JPH0821839A (en) | 1994-07-07 | 1996-01-23 | Hitachi Ltd | Sample type identifying method for automatic analyzer |
FR2730315B1 (en) | 1995-02-07 | 1997-03-21 | Abx Sa | DEVICE FOR STIRRING AND TAKING SAMPLES OF BLOOD PRODUCTS FROM TUBES GROUPED INTO CASSETTES |
US5683659A (en) * | 1995-02-22 | 1997-11-04 | Hovatter; Kenneth R. | Integral assembly of microcentrifuge strip tubes and strip caps |
US5614236A (en) * | 1995-04-07 | 1997-03-25 | Klang; Albert | Bottle closure for collecting and trapping sediment |
US5632905A (en) * | 1995-08-07 | 1997-05-27 | Haynes; John L. | Method and apparatus for separating formed and unformed components |
US5556544A (en) * | 1995-09-08 | 1996-09-17 | Didier; Emmanuel R. | Concentrator & filter |
JPH09166591A (en) | 1995-12-13 | 1997-06-24 | Sekisui Chem Co Ltd | Inverted coagulation method |
US5830154A (en) * | 1996-01-11 | 1998-11-03 | Epitope, Inc. | Device for collecting substances for testing |
GB2312746B (en) * | 1996-04-24 | 2000-07-19 | Molecular Light Technology Lim | Detection of an analyte in a Water Immiscible Solvent |
JP3604821B2 (en) | 1996-07-18 | 2004-12-22 | 大日本印刷株式会社 | Body fluid analyzer |
US5882943A (en) * | 1996-07-31 | 1999-03-16 | Aldeen; William Erick | Filtration apparatus, kit and method for processing parasite samples |
NL1003726C2 (en) | 1996-08-01 | 1998-02-05 | Micronic B V | Test tube with optically readable coding. |
US6043878A (en) | 1996-09-24 | 2000-03-28 | Case Western Reserve University | Device for optical and electrochemical measurements in microliter size samples |
US6132353A (en) | 1996-10-21 | 2000-10-17 | Winkelman; James W. | Apparatus and method for separating plasma or serum from the red cells of a blood sample |
GB9623544D0 (en) * | 1996-11-12 | 1997-01-08 | Micromass Ltd | Sample vial and vial closure device for use in gas analysis and method of using the same |
DE19703921C1 (en) * | 1997-02-03 | 1999-03-18 | Sarstedt Walter Geraete | Blood collection device |
GB2321857B (en) * | 1997-02-05 | 2000-05-24 | Intersep Ltd | Improvements in filters |
JPH10243940A (en) * | 1997-03-03 | 1998-09-14 | I R Medical:Kk | Blood collection tube |
JPH10277019A (en) * | 1997-04-02 | 1998-10-20 | I R Medical:Kk | Blood collecting tube |
KR100535381B1 (en) * | 1997-09-16 | 2005-12-09 | 세키스이가가쿠 고교가부시키가이샤 | Blood Test Container and Blood Test Method |
US6234948B1 (en) * | 1997-10-27 | 2001-05-22 | Michael Yavilevich | Combined centrifugation assembly |
JPH11318870A (en) * | 1998-05-19 | 1999-11-24 | Ir Medical:Kk | Blood drawing tube and separation of serum and plasma utilizing the same |
JP4153171B2 (en) | 1998-07-27 | 2008-09-17 | 株式会社日立製作所 | Analysis method of biological sample |
US6221655B1 (en) * | 1998-08-01 | 2001-04-24 | Cytosignal | Spin filter assembly for isolation and analysis |
JP2000084389A (en) | 1998-09-11 | 2000-03-28 | Sekisui Chem Co Ltd | Blood collecting tube stirring device |
US6497325B1 (en) * | 1998-12-05 | 2002-12-24 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6516953B1 (en) * | 1998-12-05 | 2003-02-11 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US6171261B1 (en) * | 1999-08-06 | 2001-01-09 | Becton Dickinson And Company | Specimen collection device and method of delivering fluid specimens to test tubes |
US6471069B2 (en) * | 1999-12-03 | 2002-10-29 | Becton Dickinson And Company | Device for separating components of a fluid sample |
IL134318A0 (en) * | 2000-02-01 | 2001-04-30 | Gotit Ltd | Method and apparatus for processing wine |
AT414209B (en) * | 2000-03-17 | 2006-10-15 | Greiner Bio One Gmbh | COLLECTION TANK FOR LIQUIDS |
US6602414B2 (en) * | 2000-03-30 | 2003-08-05 | Formulations Pro | Molecule separation device and method combining multiple filtration media |
DE10028482B4 (en) * | 2000-06-08 | 2004-09-16 | Kabe-Labortechnik Gmbh | Container for taking samples and in particular blood samples |
CA2416744A1 (en) * | 2000-07-18 | 2002-01-24 | Invitrogen Corporation | Device and methods for subdividing and filtering gel material and extracting molecules therefrom |
US6730071B1 (en) * | 2000-09-25 | 2004-05-04 | Alyssa J. Dassa | Collection, storage, transportation and sampling system and method of use thereof |
US8216797B2 (en) * | 2001-02-07 | 2012-07-10 | Massachusetts Institute Of Technology | Pathogen detection biosensor |
JP4569030B2 (en) | 2001-04-23 | 2010-10-27 | 東ソー株式会社 | Fluorescence detection method and apparatus capable of measurement under external light |
WO2003019131A2 (en) * | 2001-08-29 | 2003-03-06 | Hexal Pharma Gmbh | Method and device for preparing a sample of biological origin in order to determine at least one constituent contained therein |
DE60231236D1 (en) * | 2001-12-04 | 2009-04-02 | Sekisui Chemical Co Ltd | COMPOSITION FOR SEPARATING BLOOD DREAM OR PLASMA AND THIS CONTAINING VAPOR FOR BLOOD TESTING |
EP1361440A1 (en) * | 2002-05-10 | 2003-11-12 | F. Hoffman-la Roche AG | Method and apparatus for transporting a plurality of test tubes in a measuring system |
US7176034B2 (en) * | 2002-07-03 | 2007-02-13 | St. Joseph's Healthcare | Apparatus and method for filtering biological samples |
US20040059255A1 (en) * | 2002-09-23 | 2004-03-25 | Dimitrios Manoussakis | High bias gel tube and process for making tube |
EP1419820A1 (en) | 2002-11-14 | 2004-05-19 | F. Hoffmann-La Roche Ag | Method, system and reaction vessel for processing a biological sample contained in a liquid |
AU2003211930A1 (en) * | 2003-02-12 | 2004-09-06 | Atleta Incorporation | Urine sampling container |
KR20050115235A (en) * | 2003-02-13 | 2005-12-07 | 벡톤 디킨슨 앤드 컴퍼니 | Devices for component removal during blood collection, and uses thereof |
FR2858057B1 (en) | 2003-07-21 | 2006-05-26 | Abx Sa | QUALITY CONTROL DEVICE FOR BLOOD ANALYZER OPERATING IN WHOLE BLOOD |
US7488297B2 (en) * | 2003-07-30 | 2009-02-10 | Patrice Flaherty | Blood collecting devices |
BRPI0413350B8 (en) | 2003-08-05 | 2021-07-27 | Becton Dickinson Co | “device for collecting a biological sample and method for collecting and preparing a specimen” |
FR2859285B1 (en) | 2003-08-26 | 2007-08-10 | Abx Sa | HEMATOLOGICAL TOTAL BLOOD ANALYZER WITH STIRRING DEVICE |
ZA200602485B (en) | 2003-10-03 | 2007-09-26 | Novo Nordisk As | Container comprising code information elements |
US8043562B2 (en) | 2003-12-08 | 2011-10-25 | Ortho-Clinical Diagnostics, Inc. | Analyzer having removable holders or a centrifuge |
EP1694814A1 (en) * | 2003-12-08 | 2006-08-30 | Covaris, Inc. | Apparatus and methods for sample preparation |
EP1697743B1 (en) * | 2003-12-24 | 2009-03-11 | Becton, Dickinson and Company | Plasma on demand tube |
CA2458497A1 (en) * | 2004-02-24 | 2005-08-24 | Cme Telemetrix Inc. | Spectrophotometric analysis of plasma or serum in a sealed tube |
JP4098272B2 (en) | 2004-04-26 | 2008-06-11 | 株式会社アイディエス | Bar code reader for test tubes |
RU2352939C2 (en) | 2004-07-12 | 2009-04-20 | Фдг-Фон Дер Гольтц Гмбх | Device and method for automatic research of blood samples |
EP1759633A1 (en) | 2005-09-01 | 2007-03-07 | F.Hoffmann-La Roche Ag | Device for sampling bodily fluids and its fabrication method |
WO2007028860A1 (en) | 2005-09-06 | 2007-03-15 | Finnzymes Instruments Oy | Thermal cycler with optimized sample holder geometry |
US20070083130A1 (en) | 2005-09-26 | 2007-04-12 | Anne Thomson | Method for promoting bodily fluid expression from a target site |
EP1928304B1 (en) | 2005-09-30 | 2012-10-24 | Intuity Medical, Inc. | Catalysts for body fluid sample extraction |
JP4768410B2 (en) | 2005-11-15 | 2011-09-07 | シスメックス株式会社 | Stirring device and sample analyzer |
JP2007271388A (en) * | 2006-03-30 | 2007-10-18 | Hidetoshi Tsuchida | Separation method of serum or plasma, and blood separation tube |
US7449329B2 (en) * | 2006-03-31 | 2008-11-11 | Hale Anne S | Blood test kit |
US7624557B2 (en) * | 2006-05-02 | 2009-12-01 | Box Partition Technologies, Inc. | Assembling machine with continuous periodic assembly motion |
CN104634957B (en) * | 2006-05-25 | 2018-12-21 | 积水化学工业株式会社 | Container for separating the composition of serum or blood plasma and for checking blood |
US7604778B2 (en) * | 2006-06-30 | 2009-10-20 | Dause Shari L | Coded test tubes |
EP1884188A1 (en) | 2006-08-02 | 2008-02-06 | F.Hoffmann-La Roche Ag | Packaging for an object with a hydrophilic surface coating |
US8372015B2 (en) | 2006-08-28 | 2013-02-12 | Intuity Medical, Inc. | Body fluid sampling device with pivotable catalyst member |
JP2008099991A (en) | 2006-10-20 | 2008-05-01 | Olympus Corp | Blood collection device |
EP2086622A2 (en) * | 2006-10-27 | 2009-08-12 | Sierra Molecular Corporation | Penetratable septum cap |
WO2008073856A2 (en) | 2006-12-08 | 2008-06-19 | Massachusetts Institute Of Technology | Delivery of nanoparticles and/or agents to cells |
US20080164204A1 (en) * | 2007-01-08 | 2008-07-10 | Mehdi Hatamian | Valve for facilitating and maintaining separation of fluids and materials |
US7964098B2 (en) * | 2007-02-06 | 2011-06-21 | Alpha-Tec Systems, Inc. | Apparatus and method for filtering biological samples |
JP2008191070A (en) | 2007-02-07 | 2008-08-21 | Aloka Co Ltd | Sample container and sample container rack |
JP5105925B2 (en) * | 2007-03-26 | 2012-12-26 | 京セラメディカル株式会社 | Centrifugal device |
GB0706281D0 (en) * | 2007-03-30 | 2007-05-09 | Guy S And St Thomas Nhs Founda | Apparatus and method for recovering fluid from a fluid absorbing element |
NO2148743T3 (en) | 2007-05-17 | 2018-08-04 | ||
EP1995182A1 (en) | 2007-05-25 | 2008-11-26 | F.Hoffmann-La Roche Ag | A sealing cap for a fluid container and a blood collection device |
CN101688874A (en) * | 2007-07-12 | 2010-03-31 | 希森美康株式会社 | Specimen container |
US9439630B2 (en) * | 2007-08-30 | 2016-09-13 | Siemens Healthcare Diagnostics Inc. | Non-visible detectable marking for medical diagnostics |
JP5022854B2 (en) * | 2007-10-04 | 2012-09-12 | 日立アロカメディカル株式会社 | Blood collection method and blood collection coupler |
WO2009081405A2 (en) | 2007-12-25 | 2009-07-02 | Rapidx Ltd. | Devices and methods for reduced-pain blood sampling |
KR101569705B1 (en) * | 2008-01-07 | 2015-11-17 | 1크라이요바이오 악티엔게젤샤프트 | A storage vessel and a break tool for dividing such vessel |
CN103393427B (en) | 2008-03-07 | 2015-05-13 | 贝克顿·迪金森公司 | Flashing flow blood collecting needle |
JP5516415B2 (en) * | 2008-11-07 | 2014-06-11 | 日立化成株式会社 | Method for separating serum or plasma |
KR100920914B1 (en) * | 2009-05-09 | 2009-10-12 | 주식회사 무한기업 | Separable test tube used in the centrifugal separator |
WO2010132823A2 (en) * | 2009-05-15 | 2010-11-18 | Biomerieux, Inc. | System and methods for rapid identification and/or characterization of a microbial agent in a sample |
US8480953B2 (en) | 2009-05-20 | 2013-07-09 | Protedyne Corporation | System and method for vessel alignment |
CN201454557U (en) * | 2009-07-31 | 2010-05-12 | 成都瑞琦科技实业有限责任公司 | Separator tube for blood serum and blood corpuscle |
US9322761B2 (en) * | 2009-08-13 | 2016-04-26 | Siemens Healthcare Diagnostics Inc. | Methods and apparatus for ascertaining interferents and physical dimensions in liquid samples and containers to be analyzed by a clinical analyzer |
JP5484849B2 (en) | 2009-09-30 | 2014-05-07 | シスメックス株式会社 | Blood sample processing apparatus and blood sample processing method |
KR101069877B1 (en) * | 2009-10-28 | 2011-10-05 | 임기표 | Kit of centrifuge separation and methods for centrifuging using the same |
US8585905B2 (en) * | 2010-02-26 | 2013-11-19 | Waters Technologies Corporation | Devices, kits and methods for performing chemical processing |
CN102933949A (en) * | 2010-03-30 | 2013-02-13 | 巴特尔纪念研究所 | Buffy coat separator float systems and methods |
WO2011121454A2 (en) * | 2010-03-31 | 2011-10-06 | Spartan Bioscience Inc. | Direct nucleic acid analysis |
US8550273B2 (en) * | 2010-08-31 | 2013-10-08 | Wheaton Industries, Inc. | Cryogenic vials |
CN103384833B (en) | 2010-11-29 | 2014-10-15 | 株式会社日立高新技术 | Automatic analytical apparatus |
JP2014508295A (en) * | 2011-02-17 | 2014-04-03 | ネステク ソシエテ アノニム | Apparatus and method for isolating leukocytes and tumor cells by filtration |
US20120223027A1 (en) * | 2011-03-02 | 2012-09-06 | Jonathan Lundt | Tube and float systems |
WO2012138420A2 (en) * | 2011-04-08 | 2012-10-11 | Rarecyte, Inc. | Systems and methods for harvesting target particles of a suspension |
BR112013024764A2 (en) * | 2011-05-05 | 2019-05-21 | Anpac Bio-Medical Science Co., Ltd. | apparatus, device and microdevice for detection of tumor cells and for interaction with biological matter |
EP2726842B1 (en) * | 2011-06-30 | 2016-03-16 | 3M Innovative Properties Company | Systems and methods for detecting an analyte of interest in a sample using microstructured surfaces |
US9095798B2 (en) * | 2011-08-19 | 2015-08-04 | Microaire Surgical Instruments, Llc | Centrifuge separation method and apparatus using a medium density fluid |
US9810704B2 (en) | 2013-02-18 | 2017-11-07 | Theranos, Inc. | Systems and methods for multi-analysis |
TR201109999A2 (en) * | 2011-10-10 | 2012-07-23 | Akman Serhan | Tube for platelet-rich fibrin production. |
JP5808653B2 (en) | 2011-11-18 | 2015-11-10 | シスメックス株式会社 | Blood cell counter and blood cell counter method |
KR200462858Y1 (en) * | 2011-12-06 | 2012-10-05 | 이정민 | Container for centrifuge |
US9134203B2 (en) * | 2011-12-28 | 2015-09-15 | Abbott Laboratories | Blood sample tube indicator |
BR112014018970A8 (en) * | 2012-02-02 | 2017-07-11 | Becton Dickinson Co | SAMPLE COLLECTION DEVICES WITH BLOOD STABILIZING AGENTS |
US9251393B2 (en) | 2012-02-03 | 2016-02-02 | Siemens Healthcare Diagnostics Inc. | Barcode reading test tube holder |
BR112014021135B1 (en) | 2012-02-24 | 2021-03-02 | Instrunor As | fully automated cell pretreatment process instrument for preparing at least one cell sample and carousel and centrifuge arrangement |
KR101459109B1 (en) * | 2012-05-21 | 2014-11-12 | 한국과학기술원 | Container for multiple centrifugation and Particle Separation Method Using the Same |
US9476894B2 (en) | 2012-05-28 | 2016-10-25 | Hitachi High-Technologies Corporation | Centrifuge module, preprocessing system having centrifuge module, and control method for the system |
CN102764133A (en) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
JP6014424B2 (en) | 2012-08-30 | 2016-10-25 | シスメックス株式会社 | Stirring device and sample analyzer |
KR102221948B1 (en) | 2012-09-06 | 2021-03-02 | 테라노스, 인코포레이티드 | Systems, devices, and methods for bodily fluid sample collection |
WO2014050021A1 (en) | 2012-09-28 | 2014-04-03 | 富士フイルム株式会社 | Centrifugation container |
US9103749B2 (en) * | 2012-10-11 | 2015-08-11 | Fast Forward Forensics, LLC | Biological sample collection apparatus |
US9260763B2 (en) | 2012-10-22 | 2016-02-16 | Qiagen Gaithersburg, Inc. | Sample processing method using tube strips and tube strip holder |
US10166009B2 (en) * | 2012-11-20 | 2019-01-01 | The Trustees Of Columbia University In The City Of New York | Medical apparatus and method for collecting biological samples |
EP2940477B1 (en) | 2012-12-26 | 2019-02-20 | Hitachi High-Technologies Corporation | Automatic analyzer |
JP5911443B2 (en) | 2013-03-06 | 2016-04-27 | シスメックス株式会社 | Blood coagulation analyzer and blood coagulation analysis method |
CN114137240A (en) | 2013-03-15 | 2022-03-04 | 雅培制药有限公司 | Automated diagnostic analyzer with rear accessible track system and related methods |
WO2014149854A1 (en) | 2013-03-19 | 2014-09-25 | Walterspiel Juan Nepomuc | Devices and methods to reduce contamination of fluid collected from a patient |
WO2014172232A1 (en) * | 2013-04-15 | 2014-10-23 | Becton, Dickinson And Company | Medical device for collection of a biological sample |
ES2716114T3 (en) * | 2013-05-24 | 2019-06-10 | Occam Biolabs Inc | System and procedure to collect a nucleic acid sample |
CN103308376B (en) | 2013-07-11 | 2015-11-18 | 天津海迈医用科技有限公司 | Blood collection uniform mixer |
WO2015017701A1 (en) * | 2013-08-01 | 2015-02-05 | Ancestry.Com Dna, Llc | Sample collection device |
US9816087B2 (en) * | 2013-09-12 | 2017-11-14 | CellectGen, Inc. | Biofluid collection and filtration device |
JP5896571B2 (en) * | 2013-10-15 | 2016-03-30 | キム ホンKim Hong | Apparatus and method for extracting highly concentrated plasma from whole blood |
JP5946866B2 (en) * | 2014-05-09 | 2016-07-06 | キム ホンKim Hong | Highly concentrated plasma extractor |
CN203965173U (en) | 2014-06-26 | 2014-11-26 | 深圳市同盛医疗设备有限公司 | Sample cup and glass stand |
CN104031831B (en) * | 2014-07-01 | 2019-10-15 | 北京圣浦博大生物科技有限公司 | A kind of separation method of haemocyte separating pipe and mononuclearcell |
EP3006943B1 (en) | 2014-10-07 | 2020-04-22 | Roche Diagniostics GmbH | Module for a laboratory sample distribution system, laboratory sample distribution system and laboratory automation system |
EP3165921A1 (en) * | 2015-11-05 | 2017-05-10 | Dominik Olbrzymek | A kit for centrifugal separation of biological fluid components and a method for centrifugal separation of biological fluid components |
AU2016363739B2 (en) | 2015-12-03 | 2019-09-19 | Shell Internationale Research Maatschappij B.V. | Method of removing CO2 from a contaminated hydrocarbon stream |
CN113751095B (en) | 2015-12-11 | 2024-01-09 | 巴布森诊断公司 | Sample container and method for separating serum or plasma from whole blood |
US11166658B2 (en) | 2016-07-28 | 2021-11-09 | Invitae Corporation | Blood sampling system and method |
CN110213992A (en) | 2016-11-14 | 2019-09-06 | 美国西门子医学诊断股份有限公司 | Blood collection device with integrated form absorbent material |
CN110291377B (en) | 2016-11-14 | 2023-09-05 | 巴布森诊断公司 | Sample preparation device |
US20190350808A1 (en) | 2016-11-14 | 2019-11-21 | Siemens Healthcare Diagnostics Inc. | Sample collection kit for positive sample identification |
-
2016
- 2016-12-12 CN CN202110946196.3A patent/CN113751095B/en active Active
- 2016-12-12 US US16/061,309 patent/US10870110B2/en active Active
- 2016-12-12 ES ES16874075T patent/ES2846863T3/en active Active
- 2016-12-12 EP EP16874075.1A patent/EP3386391B1/en active Active
- 2016-12-12 EP EP20209543.6A patent/EP3847965A1/en active Pending
- 2016-12-12 WO PCT/US2016/066236 patent/WO2017100798A1/en active Application Filing
- 2016-12-12 CN CN201680081591.7A patent/CN108601565B/en active Active
-
2020
- 2020-10-28 US US17/082,759 patent/US11697114B2/en active Active
-
2023
- 2023-05-25 US US18/323,830 patent/US20240042427A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN108601565A (en) | 2018-09-28 |
CN113751095A (en) | 2021-12-07 |
EP3386391A4 (en) | 2018-10-17 |
CN113751095B (en) | 2024-01-09 |
US20210039088A1 (en) | 2021-02-11 |
EP3847965A1 (en) | 2021-07-14 |
US10870110B2 (en) | 2020-12-22 |
ES2846863T3 (en) | 2021-07-29 |
EP3386391B1 (en) | 2020-11-25 |
CN108601565B (en) | 2021-09-07 |
US20180353952A1 (en) | 2018-12-13 |
WO2017100798A1 (en) | 2017-06-15 |
US11697114B2 (en) | 2023-07-11 |
EP3386391A1 (en) | 2018-10-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20240042427A1 (en) | Specimen container and centrifugation method for separating serum or plasma from whole blood therewith | |
US4046699A (en) | Access device for centrifugal separation assemblies | |
US9694359B2 (en) | Mechanical separator for a biological fluid | |
EP1106250B1 (en) | Device for separating components of a fluid sample | |
KR102638609B1 (en) | isolation tube | |
JP5385383B2 (en) | Density phase separator | |
US7972578B2 (en) | Device and method for separating components of a fluid sample | |
CN100515568C (en) | Device and methods for collection of biological fluid sample and treatment of selected components | |
EP0638171A1 (en) | Flow restrictor-separation device. | |
JPH01318959A (en) | Piston type filter and dispenser vial | |
US4364903A (en) | Contamination-free separation device | |
EP3060925B1 (en) | Kit, method and assembly for preparing a sample | |
JP6018252B2 (en) | Mechanical separator for biological fluids | |
WO2013137756A1 (en) | Pipette tip for centrifuge with two closures | |
CA2899673C (en) | Mechanical separator for a biological fluid | |
CA2899672A1 (en) | Mechanical separator for a biological fluid |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: BABSON DIAGNOSTICS, INC., TEXAS Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SIEMENS HEALTHCARE DIAGNOSTICS INC.;REEL/FRAME:063869/0186 Effective date: 20200605 Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC., NEW YORK Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:OLSON, ERIC;REEL/FRAME:063869/0176 Effective date: 20160418 |
|
STPP | Information on status: patent application and granting procedure in general |
Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION |
|
STPP | Information on status: patent application and granting procedure in general |
Free format text: NON FINAL ACTION MAILED |