CN102764133A - Vacuum blood collection tube and method thereof capable of directly separating blood plasma - Google Patents
Vacuum blood collection tube and method thereof capable of directly separating blood plasma Download PDFInfo
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- CN102764133A CN102764133A CN201210285438XA CN201210285438A CN102764133A CN 102764133 A CN102764133 A CN 102764133A CN 201210285438X A CN201210285438X A CN 201210285438XA CN 201210285438 A CN201210285438 A CN 201210285438A CN 102764133 A CN102764133 A CN 102764133A
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Abstract
The invention relates to the technical field of clinical medical instruments, in particular to a vacuum blood collection tube and a method thereof capable of directly separating blood plasma. The vacuum blood collection tube comprises a blood collection tube body, an inner tube, a rubber plug and a filter element. The vacuum blood collection tube is characterized in that the blood collection tube inner tube is arranged in the blood collection tube body, the rubber plug is disposed at the top of the blood collection tube body, the structure of the rubber plug is close to a plug shape, a semicircle groove is disposed at the center of the upper portion of the rubber plug structure, left and right ends of the lower portion of the rubber plug are embedded into the blood collection tube body to be connected with the blood collection tube inner tube, a blood plasma outlet is disposed in the middle of the bottom of the blood collection tube inner tube, a blood plasma collecting chamber is mounted below the blood plasma outlet, a filtering membrane is arranged on the upper portion of the blood plasma outlet of the blood collection tube inner tube, the filter element is arranged on the upper portion of the filtering membrane, a buffer plate is mounted on the filter element, consequently a blood collection chamber is formed in the blood collection tube inner tube, and a layer of anticoagulant is disposed on the inner wall of the blood collection tube inner tube. The vacuum blood collection tube and the method thereof capable of directly separating the blood plasma have the advantages that physical form differences of blood visible components and plasma components are utilized, the blood plasma can be rapidly separated, and the vacuum blood collection tube and the method thereof are simple, convenient, safe and rapid.
Description
[technical field]
The present invention relates to clinical treatment technique with the apparatus field, but specifically be a kind of vacuum test tube and method thereof of direct separation blood plasma.
[background technology]
The detection of blood preparation is the routine techniques means of medical institutions' Clinical Laboratory; The blood preparation that is used for in-vitro diagnosis mainly is divided into arterial blood and venous blood; The arterial blood BIAO and BEN is mainly used in the vim and vigour index analysis clinically; And the detection range of application of venous blood BIAO and BEN is quite extensive, as is used to detect biochemical indicator, blood clotting index, hemorheology index, blood routine analysis, trace element and ion component analysis, nutritional labeling detection, drug level detection, gene test, the detection of infectious disease nosetiology, microorganism detection or the like.Because the different differences that detect index institute use detecting instrument, the venous blood BIAO and BEN is divided into three types of blood plasma, serum and whole bloods again according to purposes.The acquisition of plasma specimen mainly is the approach through centrifugalize traditionally, handles through the anticoagulant anticoagulant after blood exsomatizes, and isolates plasma specimen through centrifuge reaching under the condition of certain centrifugal force.
In the prior art on the market common vacuum test tube form by blood taking tube body, plug and skull, additive, label etc.; Itself do not have the function of direct separation blood plasma; If need separated plasma after blood sampling, to accomplish by centrifuge, the blood of handling through anticoagulant need pass through centrifugally just can isolate blood plasma, if centrifugal condition is grasped and badly may be formed haemolysis on the one hand; Perhaps cause the breakage of collection container in the centrifugal process, cause BIAO and BEN seepage or damage fully; On the other hand in centrifugal process and after separating; Other visible components of blood plasma and blood mix, and are receiving under the action of centrifugal force or in the process of depositing, endoerythrocytic material can be discharged in the blood plasma; Testing result to final impacts, and is safe and reliable inadequately.
But the vacuum test tube (publication number CN 202173399 U) that also has a kind of direct separation blood plasma in the prior art; Blood is quantitatively drawn back the adding in the blood sample preservation container that fills the liquid anticoagulant, fully mix homogeneously by the adsorbing material that is built in the sampling component.The separating plasma assembly that filter element is housed is inserted blood sample preserve container; The top of separating plasma assembly is a hollow elastic piston; The inwall that the outside of piston and blood sample are preserved container forms a slightly closed chamber of interference, and filter element is equipped with in hollow piston inside, through the top-down motion of piston; When piston passes through the anticoagulated blood BIAO and BEN of collection; Because the pressure effect, the blood plasma part of anticoagulated blood can be in the top of filter element through filter element, can not then still be in the bottom of filter element through the blood formed element of filter element.But the operational approach of this evacuated collection is comparatively complicated, and the operating technology to operator has certain requirement in the process of collection plasma sample, and the time that spends in the gatherer process is also longer, and efficient is not high, and is practical inadequately.
[summary of the invention]
The present invention is exactly in order to solve deficiency and the defective that blood collection container in the prior art can't direct separation blood plasma, but the vacuum test tube and the method thereof of a kind of novel structure, safe and reliable direct separation blood plasma are provided.
For realizing above-mentioned purpose; But a kind of vacuum test tube of direct separation blood plasma is provided; Comprise blood taking tube body, interior pipe, plug, filter element, it is characterized in that the top of described blood taking tube body is provided with plug, the structure proximate of plug is the plug shape; The center upper portion of plug structure is provided with semi-circular recesses; Plug bottom right ends embeds connection blood taking tube body and is connected with the interior pipe of the blood taking tube in the blood taking tube body, and the middle plasma flow that is provided with in the bottom of pipe exports in the blood taking tube, and the bottom of plasma flow outlet is the plasma collection chamber; The top of pipe plasma flow outlet is provided with filter membrane in blood taking tube; The top of filter membrane is provided with filter element, also is provided with buffer substrate tablet on the described filter element, in blood taking tube, constitutes in the pipe thus by the Blood Sampling Room between the pipe top in filter element top to the blood taking tube; Be provided with one deck anticoagulant on the inwall of in blood taking tube, managing, described anticoagulant comprises the stabilizing agent of heparin sodium, Lithium acid heparin, sodium citrate, ethylenediamine tetraacetic acid,dipotassium salt, ethylenediaminetetraacetic acid tripotassium, disodiumedetate, potassium oxalate, Disodium oxalate., hirudin and target detection thing.
Described blood taking tube body is by one or more compound the processing in glass, polyester plastic cement, the macromolecular material.
The manufactured materials of its body of pipe is mainly macromolecular material in the described blood taking tube, comprises high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene, polystyrene, Merlon, polyethylene, terephthalate.
The manufactured materials of described filter element comprises ultra-fine Plant fiber, synthetic fiber, polyester sintered product, filter paper, optimization polypropylene synthetic fibers, polyethylene synthetic fibers, polyethylene terephthalate synthetic fibers, carboxymethyl cellulose, microcrystalline Cellulose, the Plant fiber through processing.
The material of described plug comprises natural rubber, halogenated butyl rubber, synthetic rubber, thermoplastic elastomer (TPE).
Described filter element top is provided with one deck buffer substrate tablet, and the padded coaming of buffer substrate tablet comprises freeze dried anticoagulant tabletting, nylon membrane, filter paper, synthetic cellulose film, also is provided with the anticoagulant of one deck coating on the buffer substrate tablet.
Described filter membrane is a microporous filter membrane, and its manufactured materials comprises cellulose acetate, celluloid, mixed cellulose ester, nylon 6, nylon 66, Kynoar, politef, polyether sulfone, polypropylene material.
But a kind of method of vacuum test tube of direct separation blood plasma; It is characterized in that this method utilizes blood plasma different with the physical aspect of blood formed element, the interior pipe that filter element is housed is installed in that blood taking tube is inner, be separated into Blood Sampling Room and plasma collection chamber to blood taking tube inside; Anticoagulant and filter element through pipe in being built in; The blood that flows into Blood Sampling Room can not solidified, and the visible component of blood is kept off at an upper portion thereof by filter element, i.e. Blood Sampling Room; And plasma fraction can flow into the bottom of blood taking tube smoothly through filter element; Be the plasma collection chamber, thereby the blood plasma and the visible component of blood separated that the rubber plug for vacutainer of extracting pipe upper end in being connected discards whole interior pipe and can use the plasma specimen of plasma collection chamber to be used for various detections; In interior pipe, load filter membrane, filter element successively and be sprayed with the filter paper buffer substrate tablet of anticoagulant, inner pipe wall spraying anticoagulant with the above-mentioned interior pipe of the making blood taking tube body of packing into, places blood taking tube body top with plug, puts into tamponade after the vacuum machine preset vacuum.
Described blood taking tube body is made with borate glass; Rubber plug for vacutainer is made with chlorinated scoline; Pipe is made with high density polyethylene (HDPE) in the blood taking tube, and described filter membrane is asymmetric polysulfones filter membrane, and described filter element is the polyethylene terephthalate fabric filter element; Described buffer substrate tablet is the filter paper buffer substrate tablet that is sprayed with the ethylenediamine tetraacetic acid,dipotassium salt anticoagulant, described inner pipe wall spraying ethylenediamine tetraacetic acid,dipotassium salt anticoagulant.
The invention has the advantages that the physical aspect difference of utilizing blood formed element and plasma component; Utilize simple filtering ratio juris sharp separation blood plasma; Have easy, safe, characteristics fast; Need not by traditional centrifugal machinery and direct separation goes out qualified blood plasma, supply Clinical detection to use.It is transparent tube body but also the negative pressure that can effectively guarantee vacuum test tube that its material that adopts is not only processed the back, and material is easy to obtain, and cost is low, and is effective, economical and practical, safe and reliable.
[description of drawings]
Fig. 1 is the primary structure sketch map of vacuum test tube of the present invention;
Fig. 2 is the partial enlarged drawing of a part among Fig. 1 of the present invention;
As shown in the figure, among the figure: 1. pipe 7. anticoagulant 8. Blood Sampling Rooms 9. plugs 10. plasma flow outlet in blood taking tube body plasma collection chambers 2. 3. filter membranes, 4. filter elements 5. buffer substrate tablets 6. blood taking tubes;
Specifying Fig. 1 is Figure of abstract of the present invention.
[specific embodiment]
Below in conjunction with accompanying drawing the present invention is described further, the structure of this device and principle are very clearly concerning this professional people.Should be appreciated that specific embodiment described herein only is used to explain the present invention, and be not used in qualification the present invention.
But the invention provides a kind of vacuum test tube and method thereof of direct separation blood plasma, need just can not obtain qualified blood plasma by other equipment, its principle is to utilize blood plasma different with the physical aspect of blood formed element; At interior pipe that filter element is housed of the inner installation of blood taking tube; Be separated into Blood Sampling Room and plasma collection chamber to blood taking tube inside,, the blood that flows into Blood Sampling Room can not solidified through the anticoagulant and the filter element of pipe in being built in; The visible component of blood by the filter element retaining at an upper portion thereof; Be Blood Sampling Room, and plasma fraction can flow into the bottom of blood taking tube, i.e. plasma collection chamber smoothly through filter element; Thereby the blood plasma and the visible component of blood are separated, and the rubber plug for vacutainer of extracting pipe upper end in being connected discards whole interior pipe and can use the plasma specimen of plasma collection chamber to be used for various detections.
The blood taking tube body is processed by glass, plastics or other macromolecular materials, is round bottom or the point end, flat tubular structure, end sealing, other end opening.There is an interior pipe that is filled with filter element blood taking tube body inside, and interior pipe is processed by plastic cement or other macromolecular materials, and an end is connected with plug towards upper shed, and the other end is a hollow T shape structure, and the bottom has the plasma flow outlet; The external diameter of interior pipe is slightly less than the internal diameter of blood taking tube body, and the assembling back fits tightly with the blood taking tube inwall; Filter element is processed by Plant fiber, synthetic fibers or polyester sintered material, filter paper material, and its aperture can only filter blood plasma less than blood formed element, can not pass through other blood formed elements; The inwall of interior pipe scribbles anticoagulant, and anticoagulant also can be sprayed on and place filter element top on the other material; Padded coaming can be put as the anticoagulant carrier in the top of filter element, and filter membrane can be put in the bottom of filter element, further the effusive liquid of filter element is refiltered, to guarantee the quality of blood plasma.
Rubber plug for vacutainer is processed by elastomeric material, can seal the opening of blood taking tube body fully, and certain negative pressure can be preset in assurance vacuum test tube inside, and keeps vacuum in the pipe within a certain period of time.The bizet bulging of plug or externally put safety head is easy to extract after use, and the centre of pipe lid has circular groove, blood taking needle puncture when being convenient to take a blood sample; Plug and body contact portion have two aspects; The external diameter of last layer face and blood taking tube internal diameter of tube body coupling; Can guarantee to manage interior vacuum; The external diameter of next aspect and inner tube diameter coupling, and certain interference is arranged, can guarantee after blood plasma leaches, plug to be extracted from the blood taking tube body together with pipe in whole.
The material of making the blood taking tube body comprises silicate glass, borate glass, soda-lime glass, polyester plastic material, macromolecular material, preferred boric acid salt glass, polyethylene terephthalate, poly ethyldiol modified polyethylene terephthalate, Merlon, poly-naphthalene dicarboxylic acid glycol ester or polyethylene terephthalate and poly-naphthalene dicarboxylic acid glycol ester composite.Because it is transparent tube body but also the negative pressure that can effectively guarantee vacuum test tube that above-mentioned material is not only processed the back, material is easy to obtain, and cost is cheap relatively.
The material of pipe body comprises high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene, polystyrene, Merlon, polyethylene terephthalate or other macromolecular materials in making; Preferred high density polyethylene (HDPE); Its interior pipe of processing is comparatively transparent and soft; Can fit on the one hand, more or less freelyly after the blood plasma filtering on the other hand in blood taking tube, take out with the blood taking tube inwall.
The anticoagulant of inner tube wall spraying comprises heparin sodium, Lithium acid heparin, sodium citrate, ethylenediamine tetraacetic acid,dipotassium salt, ethylenediaminetetraacetic acid tripotassium, disodiumedetate, potassium oxalate, Disodium oxalate., hirudin etc.; For adapting to the different detection project; Like blood sugar test; Can also add the stabilizing agent of target detection things such as sodium fluoride, sodium iodoacetate, only otherwise can impact the subsequent detection index that obtains blood plasma and get final product.
The material of making filter element comprises ultra-fine Plant fiber, synthetic fiber, polyester sintered product, filter paper; Optimization polypropylene synthetic fibers, polyethylene synthetic fibers, polyethylene terephthalate synthetic fibers, carboxymethyl cellulose, microcrystalline Cellulose, Plant fiber through processing; Like degreasing cotton, the preferred micron order product in the aperture of superfine fibre, preferably polyethylene terephthalate synthetic fibers; This fiber can not form haemolysis, can not combine the target detection component in the blood.
The material of making rubber plug for vacutainer comprises natural rubber, halogenated butyl rubber, synthetic rubber, thermoplastic elastomer (TPE); Preferred halogenated butyl rubber; Not only be easy to molding, and possess enough elasticity and can effectively guarantee the vacuum that blood taking tube presets, after use, also be easy to extract.
The filter element top can add a padded coaming, is coated with anticoagulant or additive on the material, and the blood that so at first flows into blood taking tube can mix with anticoagulant rapidly, can not solidify.Padded coaming comprises tabletting, nylon membrane, filter paper, synthetic cellulose film of lyophilizing anticoagulant etc.; Preferably be coated with the filter paper of anticoagulant; The effect of padded coaming is to prevent not touch when blood from flowing into Blood Sampling Room at the beginning to be sprayed on the anticoagulant on the tube wall and to flow directly into filter element and form and to solidify; Form small grumeleuse, cause isolating blood plasma defective.
The filter element below can add one deck filter membrane; Filter membrane is a round sheet, and its external diameter equates with inner tube diameter, is processed by micropore inert hydrophobic material; The aperture is less than the blood cell component; Can effectively intercept the visible component of blood, itself can not react with any composition in the blood, can not adsorb any component in the blood yet.This material comprises the microporous filter membrane that materials such as cellulose acetate, celluloid, mixed cellulose ester, nylon 6, nylon 66, Kynoar, politef, polyether sulfone, polypropylene are processed.Preferred asymmetric PS membrane, the dissymmetrical structure of film material can be caught the cellular component in the blood and cracking not taken place, and can not form haemolysis, also can not adsorb critical biomarker.Should leave enough spaces between filter element top and the plug lower end, otherwise be difficult to adopt the blood of q.s.Also should leave enough spaces between at the bottom of filter element bottom and the blood taking tube pipe, otherwise filtering blood plasma can't hold.
After the present invention can realize taking a blood sample through above design not by centrifuge and direct separation goes out qualified blood plasma.
Embodiment 1
Make the blood taking tube body with borate glass; Chlorinated scoline is made rubber plug for vacutainer, and high density polyethylene (HDPE) is made pipe in the blood taking tube, and interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is sprayed with the filter paper buffer substrate tablet of ethylenediamine tetraacetic acid,dipotassium salt anticoagulant; Inner pipe wall spraying ethylenediamine tetraacetic acid,dipotassium salt anticoagulant; With the above-mentioned interior pipe of the making blood taking tube body of packing into, plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 2
Make the blood taking tube body with polyethylene terephthalate; Chlorinated scoline is made rubber plug for vacutainer, and high density polyethylene (HDPE) is made pipe in the blood taking tube, and interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the filter paper buffer substrate tablet of heparin sodium; Inner pipe wall spraying heparin sodium anticoagulant; With the above-mentioned interior pipe of the making blood taking tube body of packing into, plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 3
Make the blood taking tube body with poly ethyldiol modified polyethylene terephthalate; Chlorinated scoline is made rubber plug for vacutainer, and high density polyethylene (HDPE) is made pipe in the blood taking tube, and interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the filter paper buffer substrate tablet of Lithium acid heparin; Inner pipe wall spraying Lithium acid heparin anticoagulant; With the above-mentioned interior pipe of the making blood taking tube body of packing into, plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 4
Make the blood taking tube body with polyethylene terephthalate; Brombutyl is made rubber plug for vacutainer, and polypropylene is made pipe in the blood taking tube, and interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the filter paper buffering scraps of paper of ethylenediaminetetraacetic acid tripotassium; Inner pipe wall spraying ethylenediaminetetraacetic acid tripotassium anticoagulant; With the above-mentioned interior pipe of the making blood taking tube body of packing into, plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 5
Make the blood taking tube body with borate glass; Brombutyl is made rubber plug for vacutainer, and high transparent polypropylene is made pipe in the blood taking tube, and interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the filter paper of sodium citrate anticoagulant; Inner pipe wall spraying sodium citrate anticoagulant; With the above-mentioned interior pipe of the making blood taking tube body of packing into, plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 6
Make the blood taking tube body with poly ethyldiol modified polyethylene terephthalate; Halogenated butyl rubber is made rubber plug for vacutainer; Low density polyethylene (LDPE) is made pipe in the blood taking tube; Interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the buffer substrate tablet that the dacron membrane of hirudin anticoagulant agent is processed, and the agent of inner pipe wall spraying hirudin anticoagulant is with the above-mentioned interior pipe of the making blood taking tube body of packing into; Plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Embodiment 7
Make the blood taking tube body with polyethylene terephthalate; Halogenated butyl rubber is made rubber plug for vacutainer; High density polyethylene (HDPE) is made pipe in the blood taking tube; Interior pipe loads asymmetric polysulfones filter membrane, polyethylene terephthalate fabric filter element successively and is coated with the potassium oxalate anticoagulant and buffer substrate tablet that the dacron membrane of sodium fluoride stabilizing agent is processed, and inner pipe wall spraying potassium oxalate anticoagulant and sodium fluoride stabilizing agent are with the above-mentioned interior pipe of the making blood taking tube body of packing into; Plug is placed blood taking tube body top, put into tamponade after the vacuum machine preset vacuum.
Make vacuum test tube by the foregoing description respectively, use venipuncture needle puncture vein, capacity blood sampling respectively, the blood sampling back is upright placed 15 minutes, transferred to rubber plug for vacutainer and interior pipe to discard in the lump.Observing the blood plasma of collecting in the blood taking tube body is faint yellow, transparent or semitransparent liquid.
Claims (9)
1. but the vacuum test tube of a direct separation blood plasma; Comprise blood taking tube body, interior pipe, plug, filter element; The top that it is characterized in that described blood taking tube body (1) is provided with plug (9); The structure proximate of plug (9) is the plug shape; The center upper portion of plug structure is provided with semi-circular recesses; Plug (9) bottom right ends embeds pipe (6) in the blood taking tube that connects in blood taking tube body (1) and the blood taking tube body (1), is provided with plasma flow outlet (10) in the blood taking tube in the middle of the bottom of pipe (6), and the bottom of plasma flow outlet is plasma collection chamber (2); The top of pipe (6) plasma flow outlet is provided with filter membrane (3) in blood taking tube; The top of filter membrane (3) is provided with filter element (4), also is provided with buffer substrate tablet (5) on the described filter element (4), in blood taking tube, constitutes in the pipe (6) thus by the Blood Sampling Room (8) between pipe (6) top in filter element (4) top to the blood taking tube; In blood taking tube, be provided with one deck anticoagulant (7) on the inwall of pipe (6), described anticoagulant comprises the stabilizing agent of heparin sodium, Lithium acid heparin, sodium citrate, ethylenediamine tetraacetic acid,dipotassium salt, ethylenediaminetetraacetic acid tripotassium, disodiumedetate, potassium oxalate, Disodium oxalate., hirudin and target detection thing.
2. but, it is characterized in that described blood taking tube body (1) is by one or more compound the processing in glass, polyester plastic cement, the macromolecular material like the vacuum test tube of claims 1 described a kind of direct separation blood plasma.
3. but like the vacuum test tube of claims 1 described a kind of direct separation blood plasma; The manufactured materials that it is characterized in that pipe (6) its body in the described blood taking tube is mainly macromolecular material, comprises high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene, polystyrene, Merlon, polyethylene, terephthalate.
4. but like the vacuum test tube of claims 1 described a kind of direct separation blood plasma; The manufactured materials that it is characterized in that described filter element (4) comprises ultra-fine Plant fiber, synthetic fiber, polyester sintered product, filter paper, optimization polypropylene synthetic fibers, polyethylene synthetic fibers, polyethylene terephthalate synthetic fibers, carboxymethyl cellulose, microcrystalline Cellulose, the Plant fiber through processing.
5. but, it is characterized in that the material of described plug (9) comprises natural rubber, halogenated butyl rubber, synthetic rubber, thermoplastic elastomer (TPE) like the vacuum test tube of claims 1 described a kind of direct separation blood plasma.
6. but like the vacuum test tube of claims 1 described a kind of direct separation blood plasma; It is characterized in that described filter element (4) top is provided with one deck buffer substrate tablet (5); The padded coaming of buffer substrate tablet comprises freeze dried anticoagulant tabletting, nylon membrane, filter paper, synthetic cellulose film, also is provided with the anticoagulant of one deck coating on the buffer substrate tablet.
7. but like the vacuum test tube of claims 1 described a kind of direct separation blood plasma; It is characterized in that described filter membrane (3) is a microporous filter membrane, its manufactured materials comprises cellulose acetate, celluloid, mixed cellulose ester, nylon 6, nylon 66, Kynoar, politef, polyether sulfone, polypropylene material.
8. but the method for the vacuum test tube of a direct separation blood plasma; It is characterized in that this method utilizes blood plasma different with the physical aspect of blood formed element, the interior pipe that filter element is housed is installed in that blood taking tube is inner, be separated into Blood Sampling Room and plasma collection chamber to blood taking tube inside; Anticoagulant and filter element through pipe in being built in; The blood that flows into Blood Sampling Room can not solidified, and the visible component of blood is kept off at an upper portion thereof by filter element, i.e. Blood Sampling Room; And plasma fraction can flow into the bottom of blood taking tube smoothly through filter element; Be the plasma collection chamber, thereby the blood plasma and the visible component of blood separated that the rubber plug for vacutainer of extracting pipe upper end in being connected discards whole interior pipe and can use the plasma specimen of plasma collection chamber to be used for various detections; In interior pipe, load filter membrane, filter element successively and be sprayed with the filter paper buffer substrate tablet of anticoagulant, inner pipe wall spraying anticoagulant with the above-mentioned interior pipe of the making blood taking tube body of packing into, places blood taking tube body top with plug, puts into tamponade after the vacuum machine preset vacuum.
9. but the method for the vacuum test tube of a kind of direct separation blood plasma as claimed in claim 8; It is characterized in that described blood taking tube body makes with borate glass; Rubber plug for vacutainer is made with chlorinated scoline; Pipe is made with high density polyethylene (HDPE) in the blood taking tube, and described filter membrane is asymmetric polysulfones filter membrane, and described filter element is the polyethylene terephthalate fabric filter element; Described buffer substrate tablet is the filter paper buffer substrate tablet that is sprayed with the ethylenediamine tetraacetic acid,dipotassium salt anticoagulant, described inner pipe wall spraying ethylenediamine tetraacetic acid,dipotassium salt anticoagulant.
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| CN201210285438XA CN102764133A (en) | 2012-08-10 | 2012-08-10 | Vacuum blood collection tube and method thereof capable of directly separating blood plasma |
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