JP2001181167A - Inhibitor for elastase activity and cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new and safe inhibitor for an elastase activity and a cosmetic composition having an inhibitory action for the elastase activity and capable of giving glossiness and tension to the skin, preventing aging, reducing wrinkle and skin looseness and maintaining a state of youthful and fresh skin. SOLUTION: The invention provides the inhibitor for the elastase activity and the cosmetic composition containing an extract of one or more seaweeds selected from a brown algae (Padina arborescens, Laminaria japonica, Hizikia fusiforme, Fucus Vesiculosus and Undaria pinnatifida), a red algae (Eucheuma muricatum, Chondrus elata, Gigartina tenella, Grateloupia sparsa and Gelidium amansii) and a green algae (Enteromorpha linza and Ulva pertusa).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to one kind selected from brown algae (Umuchiwa, Kombu, Hijiki, Hibamata, Wakame), red algae (Kirinsai, Kotoji nonomata, Suginori, Hirimen, Tengusa), and green algae (Aonori, Anaaosa). The present invention relates to an elastase activity inhibitor or a cosmetic composition containing the above seaweed extract.

[0002] The field of application is, for example, applicable to all kinds of external preparations (including preparations for animals), and specifically, ampules, capsules, pills, tablets, powders, granules, solids, 1) Pharmaceuticals, 2) of liquid, gel or air bubbles
Quasi-drugs, 3) topical or systemic skin cosmetics (e.g., basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers and skin cleansers, massage agents) , Cleansing agents, depilatory, depilatory, shaving treatment, aftershave lotion, pre-show lotion, shaving cream, foundation, lipstick, blusher, eyeshadow, eyeliner, makeup cosmetics such as mascara, perfumes, Cataplasms, plasters, tapes, sheets, patches, aerosols, etc.), 4) Medicinal or /
And cosmetic preparations (eg, shampoos, rinses, treatments, permanent liquids, hair dyes, hair dressings, hair tonics, hair growth / hair restoration, cataplasms, plasters, tapes, sheets, patches) , Aerosols, etc.), 5) bath agents for use in bath water, 6) other, axillary deodorants and deodorants, deodorants, hygiene products, sanitary cotton, wet tissues, mouth fresheners (eg toothpaste, Mouse wash, etc.).

[0003]

2. Description of the Related Art A typical change in the aging of human skin is wrinkles and lumps, which are one of the major worries for the elderly and women. However, at present, there are few effective treatments for skin wrinkles and tarmi, and various studies on aging are beginning to take place in an aging society.

[0004] At present, it is considered that the formation of wrinkles and lumps on human skin is caused by a decrease in the elasticity of the skin. However, specific histological phenomena include:
Changes in extracellular matrix components such as elastin, collagen, and glycosaminoglycan in the dermis are mentioned, and it is known that changes in elastin are particularly important.

[0005] In other words, it is thought that the decrease in skin elasticity that occurs during the formation of wrinkles and tarmi involves the elastase produced and secreted from dermal fibroblasts being involved in the denaturation of the three-dimensional structure due to elastin damage. It is important to suppress the activity of elastase and to prevent denaturation of elastin, which maintains the elasticity and firmness of the skin, to prevent the formation of wrinkles and tarmi, that is, to prevent aging of the skin.

[0006]

Accordingly, the present invention suppresses the activity of elastase, restores and maintains the firmness and elasticity of the skin, thereby preventing the aging of the skin, and providing a youthful skin without wrinkles and lumps. It is an object of the present invention to provide an elastase activity inhibitor (anti-aging agent) having an effect of maintaining the activity.

[0007]

In view of these circumstances, the inventors of the present invention have developed a useful seaweed having an elastase activity inhibitory effect as a theme of development, and as a result, have found brown algae (Umiuchiwa,
1 selected from kombu, hijiki, hibamata, seaweed), red algae (giraffe rhinoceros, kotoji nonomata, sugino-ri, limen, tengusa), and green algae (aonori, anaoaosa)
It was confirmed that more than one species of seaweed extract significantly suppressed the activity of elastase. And give the skin gloss and firmness. In order to complete the present invention, the present invention provides a highly safe elastase activity inhibitor or a cosmetic composition which can maintain the skin without wrinkles or bulges since it prevents denaturation of elastin which is an elastic fiber. Reached.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The term "Umiuchiwa" used in the present invention refers to a brown algal plant phylum (Phaeophyta), a homomorphic class (Isogene).
ratae), Aphididae (Dictyotales), Aphididae (Di
ctyotaceae), classified into the genus Padina. The body is flat and fan-shaped, has no middle ribs, and the body is initially single but may grow and split into several fragments. The growth of the body is engulfed on one side of the body by a large number of meristem cells arranged in a row on the edge.
The body consists of one to several layers of cells, with concentric rows of hair. The base of the body is covered with brown false feathers in a felt-like manner. Umiuchiwa (Pb
oryana Thivy in Taylor), Konau Miuchiwa (P.crassa Y
amada), Okinawa prickly (P. japonica Yamada), Usuki prickly pear (P. minor Yamada), Etch prickly pear (P. stipita
ta Tanaka et Nozawa in Tanaka), P. ryukyuana YP Le
e et Kamura etc. are listed as representatives.

[0009] "Kombu" is a brown algal plant phylum (Phaeophyta),
Heterogeneratae, Laminariale
s), Laminariaceae (Laminariaceae), classified into the genus Laminaria (Laminaria), a single leaf with no hair plexus, no mucous cavity, no middle ribs on the leaves, roots are fibrous, stems refer to algal bodies that do not branch, for example ,
Macomb (L. japonika Areschoug), Resiliconbu (L. jap
onica Areschoug var.ochotensis Okamura), L. religiosa Miyabe, L. angusta
ta Kjellman) is a typical example.

"Hijiki" refers to a brown algal plant phylum (Phaeophyt).
a), Meridian rope (Cyclosporeae), Hibamata (Fucales), Hondara (Sargassaceae), genus Hizikia (Hizikia). The underwater forest has roots, stems, leaves, and bubbles (floating bladders). Bubbles are typically represented by bulging cylindrical twigs to form a spindle or club.

"Hibamata" refers to a brown algal plant phylum (Phaeophyt).
a), the class of the circle (Cyclosporeae), the order of the cypress (Fucales), the family of the cypress (Fucaceae), classified into the genus Hibama (Fucus), the cypress (F. Vesiculosus) (or F. distichus Linn
aeus subsp.evanescens (C.Agardh) Powll), whose body is membranous, with midrib, 20-50 cm in length, cold-sea marine algae that grow in the middle and above the intertidal zone. It is raised as.

"Wakame" refers to a brown algal plant phylum (Phaeophyt).
a), Heterogeneratae, Laminariformes (Laminari
ales), Laminariaceae (Laminariaceae), Wakame (Undaria)
Algae that have hair plexuses and mucous glands in the leaves and fold at the bottom of the body, for example, seaweed (U. pinnatifida
Suringar), Hirome (U. undarioides Okamura), Blue Whale (U. peterseniana Okamura) and the like are mentioned as typical ones.

"Kirinsai" refers to Rhodophyta (Rhodoph
yta), Florideae, Giginartidae (Gigartinal
es), Myrinaceae (Solieriaceae), Giraffe (Eucheum)
Classified as a), the body is cylindrical or oblate. The narrowing of the base of the branch refers to an algal body that is light, densely formed with short conical or barbed projections, for example, E. serra J. Ag., E. amakusaensis Okamura,
(Ryukyu Tsunomata) (E.denticulata (Burman) Coll
ins et Hervey) and E.cupressoide
um WvBosse) and the like.

"Kotoji no nomata" refers to a red algal plant phylum (Rho
dophyta), Fluorideae, Gigart
inales), Gigartinaceae, Chonomata (Cho
ndrus), which has a covering tissue on the capsule, the body is crowded, flattened, bifurcated several times in the upper part of the body, and the axilla refers to a round algal body, for example, kotojitsunomata (naga (tsuno) ) Mata)
(C.elata (Holmes) Kim) and C. armatus (Ha
rvey) Okamura), Obatsunomata (C. giganteus Yend)
o), Marubatomata (C.nipponicus Yendo), Tsunomata
(C.ocellatus Holmes), C.pinnulatus (Har
vey) Okamura), Ibotsunomata (C. verrucosus Mikami),
Tochaka (Yahatsuni Mata) (C.crispus Stackhous)
e), Kurohaginnanso (Ezotsunomata) (C.yendoi Y
amada et Mikamiin Mikami) and so on.

"Suginori" refers to Rhodophytium (Rhodophyt)
a), genus Red Algae (Florideae),
s), suginoderidae (Gigartinaceae),
Classified in (a), capsules extend from the surface of the body, and the body refers to algal bodies that usually branch in a pinnate shape, for example, G. tenel
la Harvey), Shikinori (G.teedii (Roth) Lmx.), Kainori (G.intermedia Sur.) and the like can be mentioned as representatives.

"Hilimen" means Rhodophyta (Rhodoph.
yta), Fluorideae, Crachtone (Cryptone)
miales), centipedenaceae (Grateloupiaceae), genus (Grateloupia), ヂ rimen (G.sparsa (O
kamura) Chiang), Tambanori (G.elliptica Holmes),
G. turuturu Yamada is a typical example in which tetrasporangial sac are scattered in the entire cortex and have crease-like wrinkles on the body surface.

"Tengsa" means Rhodophytium (Rhodophyt)
a), the genus Red Algae (Florideae),
s), there are three generations of male and female gametes, parasitized sporophytes, and tetraploids that are exactly the same in shape as gametes, classified into the family Gelidiaceae. It refers to the algae that grow asexually and produce asexual branches,
In the genus Tengusa (Geridium), for example,
(G. amansii Lamouroux), Nettle (G. subcostatum Okam)
ura), linseed (G. japonicum Okamura), and the like in the genus Pterocladia (Pterocladia), for example,
ra), cataoba (P. densa Okamera), and the like in the genus Yatabella (Yatabella), for example, Y. hirsuta Okam
ura), etc., in the genus Acanthopeltis (Acanthopeltis), for example, iris (A. japonica Okamura), such as
idiella), for example, G. acerosa (Forssk
l) Feldmann et Hamel) is a typical example.

"Aonori" is a phylum Chlorophyt
a), Chlorophyceae, Chryophyceae, Ulvae (Ulvales), Ulvaeaceae (Ulvaceae), genus Aonori (Enteromorpha), a hollow tubular body, the wall of which consists of a single layer of cells, for example, Uzuba Aonori (E .linza J. Agardh)
(E.prolifera J.Ag.), Hira Aonori (E.compressa Grev
ille), Bow Aonori (E.intestinalis Link), Hosoeda Aonori (E.crinita J.Ag.) and the like are listed as typical ones.

"Anaosa" is a phylum Chlorop.
hyta), Chlorophyceae, Chrysophyceae (Ulvales),
The Ulva (Ulvaceae) and the Ulva (Ulva) are classified into the genus Ulsa (U. pertusa Kjellman). The one that forms a large community mainly on the rocks in the lower part of the belt is mentioned as a typical example.

The extracts of brown algae (Sea urchin, Kombu, Hijiki, Hibamata, Wakame), red algae (Kirinsai, Kotoji nonomata, Suginori, Hirimen, Tengusa) and green algae (Aonori, Anaosa) used in the present invention are each seaweed. A body that has been crushed and then squeezed. Or
Extracted with a solvent as it is or after pulverization.

Examples of the extraction solvent include water, alcohols (eg, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and ethyl. Esters such as ether, dioxane, acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene and chloroform can be used alone or in any combination of two or more. It can be used even if it is done.

Although the production method is not particularly limited, it may be usually within the range of the boiling point of the solvent at normal temperature and normal pressure.
A solution, paste, gel, or powder may be obtained by decoloring and purifying. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

In addition, various seaweed extracts of brown algae (sea seaweed, kelp, hijiki, hibamata, seaweed), red algae (giraffe rhinoceros, kotoji nonomata, suginori, pulimen, tengusa), and green algae (aonori, anaoasa) are used to inhibit elastase activity. Drying, concentration, dilution or the like may be arbitrarily performed and adjusted according to the dosage form and form of the preparation or the cosmetic composition.

The various seaweed extracts of the brown algae (Sea urchin, Kombu, Hijiki, Hibamata, Wakame), red algae (Kirinsai, Kotoji nonomata, Suginori, Hirimen, Tengusa) and green algae (Aonori, Anaoasa) of the present invention are directly used as elastase activity. In addition to being usable as an inhibitor, it can be contained in a cosmetic composition, but its content is not particularly limited, and the type, quality, and expected action of an elastase activity inhibitor or a cosmetic composition dosage form. The amount slightly varies depending on the degree, and is usually 0.001% by weight or more (hereinafter, represented by%), preferably 0.2 to 10%. The content is 0.001%
If less, the effect cannot be expected sufficiently.

The elastase activity inhibitor or the cosmetic composition of the present invention may optionally contain the following components and additives as required, in addition to the above-mentioned essential components, as long as the effects of the present invention are not impaired. Can be selected and used in combination.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )Such.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid Synthetic fatty acids such as, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

(5) Alcohols: ethanol, isopropanol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol,
Synthetic alcohols such as isostearyl alcohol and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or its ester, trehalose or its derivative, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, alkylene such as ethylene oxide (C2-C4)
Oxide-added hydroxyalkyl (C2-C
4) chitin or chitosan, low molecular weight chitin or chitosan,
Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose , Hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, Polyethyleneimine and the like.

(10) Surfactants Anionic surfactants (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactants (alkylamine, alkyl quaternary ammonium) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants, nitrogen-containing nonionic surfactants, and other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U Such.

(12) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a usual manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decoloration, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, the processing to be performed later, and the like. ,Propylene glycol,
It is preferable to use one or a mixture of two or more selected from among 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Giving flexibility, relieving irritation, relieving stress by fragrance, activating cells (preventing cell aging), suppressing inflammation, improving skin and hair quality, preventing and improving rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, relief of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as fragrance, deodorization, thickening, preservation, and buffering can be expected.

As a specific plant (crude) used as a raw material,
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsujirafuji (Kibo), Acacia, Akasho (Akasho), Akasuri `` Fruit '', Red clover, Red grape, Akaminoki (Logwood), Red acacia (Red bud), Akane (Akane grass root), Red acacia or Jio ( Ground yellow), Agi (Awei), Aki Larch, Akinire (Latinaceae),
Aguay Guacu, Aguahe (Omitengu palm),
Akebi (Kidori), Asa (John Asako), Malva Asagao or Asagao (Toshibushi), Hydrangea (hydrangea), Ashitaba (Ashitaba),
Azuki (red azuki), acerola, asenyaku (Asenyaku), acira (shokuyoukanna), anise, abiu, abiurana, rape, avemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaworu, amadocoro (tamatake), Amana (Kikomiko), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriax, Amigasa lily or Bimo (shellfish),
Aristolomeria (Yurizisen), Arcana (Alcanna), Algarrobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souduq), Aloe (Length), Aloe vera, Angelica, Apricot or Hong apricot (Anjin), Ansokkou (Azusa), Yellow sapote , Ikakozorina (ground bile head), Epimedium or Epimedium
(Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root),
Strawberry (first place), strawberry, fig (Fig.
Leaf)), Ichihatsu (first time), Ichibi (Fruit Aoi), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Carob, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, turmeric (Utsukin), duckweed (buoying), Usubasaishin or Keirinsaishin or Shousaisaishin (spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qangji, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Urajirogashi, Uwaurushi (Kusakokomomo), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezogoka), Echinashi (Hosobamurasakikirengiku) , Enishida, Enokitake (Enokitake), Ebisugusa or Cassia Tora (Keiko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Gossip, Sophoraceae rice), Ogi or Kibananagi (yellow) Hoki), Ousei (Naruko lily, Kagi Kurumabanarukoyuri), OuHisasa Yuki or Sasanoyuki, Coptis (Coptis), Oogatahouken, giant Karasuuri (Karokon), Ooguruma (civil engineering incense), giant Tsudzurafuji
(Self defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), toucan cactus, pygmy plant or okera
(White jujube), giant leaf sedge (banaba), giant leaf beetle "fruit", giant squirrel (grain sperm), giant caterpillar, giant hawthorn or hawthorn (yamashiko), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', ogulma (turnover), panax ginseng or ginseng (carrot), hypericum or hypericum perforatum or perforated periwinkle (brother cut grass), dragonfly (continuous cut), onamimi (blue) Ears), Onigurimi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasayuri or Hakatayuri (Yuri), Ononis, Halibut
(Hihayu), Ominaeshi (defeated), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, pericarp", silkworm (ripened yellow) , Cocoa "fruit, peel,
Seeds '', oysters (persimmons `` leaves ''), kagi kazura (crop hooks), oysters or kantosou (rensensou), gaku hydrangea, cassia, casino ki (crocodile fruit `` fruits ''), gajutsu (shadotsutsu), Kashiwa
(Kazuki, Kazaha), Cascarilla, Cascara sagrada, Gypsophila, Kanixa (Kin Sato), Kaniwa, Carnation,
Valerian (valerian root), birch or weeping birch (white birch), gerbera, pumpkin, kapok tree, `` seed '', kahoku sansho (pepper), gama (bamboo), chamomile or roman chamomile, chamomile (weed), camucum (duckling),
Color, Karaku Sakeman, Calla Uri or Chinese Calla Uri (Our Gourd), Callas Bishaku (Midsummer), Oat Wheat, Guarana `` Seeds '', Calla Oo (Pop), Karaya, Galyu, Karin
(Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Koryosai, Hakuboshi), Kawazusha, Kawaratake, Kawaranadeshiko (Ishitake) or Ezokawaranadeshiko (Qumugi, Quumiko), Kawaranzin (blue wheat), Kawarayomogi (Inchinchinko) , Kansui
(Sweet), licorice (licorice), cantaraasa, candelilla,
Kanto, Canna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Nawashiroi strawberry, Mapi strawberry, European strawberry), Kiwi `` Fruits, leaves '', Kikarasuuri (Urorone), Kikyo (Kikyo, Kikyo root), Kiku (Chrysanthemum, Shimakaniku, Artemisia japonica, Kikutanikiku, Catalpa (Azumitsu), Rigid ridge (Yotei root), Pheasant (Kikomi), Kizuta, Kidachi aloe, Kidachi mint, Kina, Kinoki (Shincona, Akakinoki), Quinoa (Kinoa),
Yellowfin (Hypashi), Gymnema Sylvesta, Kimmenkaku, Cabbage, Cabbage "Immature Fruit", Cucumber, Gyoryu
(Nishikawayanagi, Ichiyanagi), Kiraja Saponaria, Kiraya, Kiranso (Gold Acne Herb), Kumquat `` Fruits '', Ginseki,
Kimbusen, betel, golden hibiscus (Sesame crass), guapeba vermelha, guava `` fruit '', guayule, quelux infectria (gallicate), cucumber nuts,
Wolfberry (Gugi, gugi, gugi leaf, skeletal bark)
(Tenmen winter), Kudzu (Kakkon), camphor tree, gooseberry `` fruit '', Ginseng (yellow flower), Kutiti River, Gardenia (Gardenia), Kunugi (Bokuso), Kupuas, Kumazasa,
Black pine (horse whip grass), Clara (bitter butter), cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curculigo latifolia `` fruit '', grapefruit `` fruit / leaf '', buckthorn, black squirrel (rescue), Kurobana Hikiokoshi Or Hikiokoshi (extended lifegrass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi), kaigai (infestation, infestation), celosia (corolla, rooster), gekkavidin, bayberry (laurel), kenashisarutoribara (soil) (Bukuryo, mountain return), gentian, genoshoko (old treasured grass), kenponashi (kigushi), koukisekoku, kishumikan (kouji, tachibana, ovenimikan, fukuremican, sagamikouji, ponkan, santara (tachibana)), koshinbara (monthly flower) , Kosui mint, Kozo "fruit", Kocha (tea), Kouhone (river bone), Koho (Straw and Kara straw), Koryang, Kouryokyo (Korakyo), Cilantro `` fruit '', Kourouen (Kohren), Koganebana (yellow gon), bilberry (Koshibachi), coconut `` fruit '',
Goshuyu (goshuyu), Goshoichigo (covered bonko), pepper
(Pepper), cosmos, copaiba balsam, coffee `` seed, leaves '', kobushi or magnolia (hot spring), burdock (burdock,
Burdock), kobotdur, sesame (sesame), sesame seeds (gensin), garnish (gomiko), sanekazura or binankazura or matsubusa, wheat (wheat), rice or rice bran "red bran, white bran", rice oil, cola acuminata " Seeds ", Cola
Vera `` seed '', colony lya, fenugreek `` fruit '', colombo, kondurango, konjac, comfrey (finnari grass),
Sisal (Sisal Asa), Sakisima button or Sinosa button or Shina sensou (weird spirit), Sakura (Oshimazakura, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Chojizakura, Koganizaka `` Flowers, fruits, bark (cherry bark) ''), cherries, pomegranate, sasa, sasanqua, sagemodaka (sawaka), sweet potato, sugar cane, sugar beet, sanebu jujube (acid jujube), saffron (red safflower, west safflower), sapodilla, sapote mariyo , Pomelo "fruit", Soapwort, Salmonberry "fruit", Sarasin
(Masame), Salvia (Sage), Sawakikyo (Yamakona), Sawagumi (Yamawomo), Sankaku Cactus, Sangre degrad (Croton), Sansouyousou, Sanshinjinjin
(37 ginseng), sanshuyu (sanshuyu), sansho (sansho),
Sandscon (Yamazone), Shea (Karite), Cyanoki `` fruit '', Shiitake (Shiitake), Shion (Shion), Dikitaris, Shikunshi (Kimiko), Perilla or Aodiso or Chirimenjiso or Kamenjiso (Shiso leaf, Shiso), Rosewood, linden,
Chinese pine, Chinese forsythia (forsythia), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiiroshimeji,
Bunashimeji, honjimeshi, shirotamogitake), shimokuren (hot spring), shimotsukeisou, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), shukukonemisou, shukushamitsu (sunin, Shredded sand), jujudama, palm `` fruit '', ginger (ginger), giant hydrangea (Tsuneyama), shobu (iris, iris root), shojiku `` fruit '', shirakashi `` seed '',
White butterfly (seed), white clover (clover), white adzuki (chicken bone grass), white iris (small iris), white natto (flower), white nanten (minamitenmi),
Cinchona Sacrifura, Jinjuge (Mizuka, Mizuka,
Cinnamon), cinnamon, cinnamomum cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flowers, shinto winter), sorrel (acid model), swimberry "fruit", star apple, stevia, strawberry "fruit", tin shiko ,
Horsetail (Thorn), Purslane (Horse Tongue, Horse Tongue), Plum (Fruit), Pinus sylvestris (Cones), Larix sylvestris, European Lentils, European Walnut, European Hawthorn, European Dandelion, European Chestnut (Marronnier), Pear "fruit", snow pear,
Sambucus elder (Elder), Atlantic shrew (Juniper, Juniper), Achillea millefolium (Mirfoil), Atlantic rose, Aurora magnolia, Atlantic mistletoe, Western mackerel or Western mackerel, Western horseradish, Sekisho (stone irises) Cedron (Bowshukuboku), Zeniaoi, Hirohasenega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shingenren), Sendan, Sennin Cactus, Senna `` Fruits, Leaves '', Senninsou (Otatate), Senbri ), Souka (grass), elephant, buckwheat `` seed '', soybean potato, rhubarb (daisuki), radish, soybean, daidai (orange skin, kikomi), takasaburo (dry lotus grass), takatodai (daigeki), takawarabi (dog) Spine), dark sweet cherry "fruit", hollyhock, hollyhock (thyme) HyakuRika), Tachidokoro (explicit solution), Tachiyanagi, tamarind "seed", onions, Magnolia salicifolia (fist), Aralia elata "fruit, leaf, root bark",
Dahlia (Tengiku button), Tarui, Tanshiniwa, Tanjin (Dan ginseng), Dandelion (Bamboo British) or Shirobana dandelion or Moko dandelion, Dammar, Cherry `` fruit '', Tigaya `` fruit, root, bud '', Chikusetsu carrot (Bamboo ginseng),
Chicory, chancapiedra (Kidachikomikansou), Chinese radish (Ohio), Korean lance (Wuyi), Korean sagebrush (Aiba), Choreimaitake (Irei), Chorogi, Tsukimisou, Tsukuritake (mushroom), camellia, camellia, clover (Lacquer mother grass), twigweed (duckling grass),
Tsuruazuki (red azuki bean), tsuldokudami (how many crows), tsuruna
(Kankyo), Carrot (four-leaf ginseng), Tsuwabuki, Deiko, Teuchigurumi, Duberry `` Fruit '', Tulip, Chocolate stay, Tengusa, Tencha (Tencha), Tendaiyaku (Wakayaku), Touga (Winter melancholy), Toukagi Kazura, Capsicum (Pepper), Touki (Toki), Calendula (Marigold), Tonabena (River cut), Maize or Maize hair (Nanban hair), Toad Mochi (Female Sadako), Toudodou or Korean gentian (Ryubile), Tokusa (wood pirates), dokudami (jujutsu), konkon (sore root), sashimi or mamedaoshi or scutellaria, eucommia (mushroom `` bark, leaves, roots ''), ash (qin bark), tomato, tragacanth, triacanthos `` seed '', Turkey Kikyo, Tormentilla, Dronoki, Trolo-mallow, Niseria berry `` fruit '', Chinese yam or Yamanoimo (herbal medicine), (Chur Bloom), Naginata Kouju, Nazuna, Rape, Rape Bean or Harbinger (Sword Bean), Natsumikan, Jujube (Dajutsu), Naniwaibara
(Kanekurako), Navena (intermittent), Nameko, Narco lily (Yoshimi),
Peanut (peanut), Nanten (Minami Tenmi), Namban Karamushi (ramie), Niois violet, Nigaki (Makiki), Nigamogi (Grasshopper), Nikuzuku, Kay or Nikkei or Ceylon Nikkei or Yabnikkei (Chicago) or Keishi (Katsushige) ), Chinese chive
(Niriko), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nurde (five times), leek, nemoki or neb or nebi or nemuriki or digokbana (health), thistle (Oji) , Noibara (fruit), Yarrow, Japanese radish (Maeko), Nobara, Nomomo, Pineapple `` fruit '', Hibiscus (Bussouge, Furinbussouge, Roselle),
Hyakudo, Hauchiwa, Pau Dosse, Hakayamauraboshi (bone crushing), Hakusen (ringworm skin), Huckleberry `` fruit '', Hakobe (growth), Hashibami (Haruko), Hashiridekoro
(Roth root), basil, lotus (lotus, lotus, lotus), parsley
(Netherland jelly), naked mussel, batata, bee or medake (bamboo boiled), patchouli, mint (lightly loaded, lightly loaded leaves), barley (yokuinin), hanasuge (chimbo), banana, hana mint, hanabishi (situri, shisuturi), vanilla bins , Papaya, japonicus (rat koji), pahalobobo, hub "whole plant, stem, leaf", paprika, hamago or honeysuckle
(Vine vine), hamasuge (katsuki), hamabishi (shikoriko), hamanas (maikai flower), hamabafu (beach storm), hamamelis, palm, rose (rose), balata, balata rubber tree,
Agaric (Agaric, Agaricus agaricus, Uskimorinosa), Haran, Pariurus (Pleurotus japonicus), Parietaria, Balsamina (Tsurureishi, Nigari), Harunire (Yellow, Yule bark, Yuba), Barbasco, Harindou, Handaikai ), Breadfruit, giant gourd (sun dried), lizard ginseng (party), pecan nuts, gangamana (stones and garlic), higuru madaria, Japanese cypress (hishomi), pistachio, beet, hitotsuba (stone reed), tsutsubayashida , Daisies (daisy), hinotaino kozuchi (cow knees), hinoki, cypress, castor, sunflower, bell pepper, himeuzu (tenoi), himega (perfume), himematsutake (kawariharatake, hiromatsutake), pimenta `` fruit '', juniper, beakyuu , Hiyu (fruit), below-flower, Hiroha okinagusa (bakuin), loquat (fruit, leaf) Stalks '', betel nut (large clothing skin, areca locust), Pu'er tea (Pulse tea), Sperm kazura (Southern rattan), Butterfly, Butterfly dandelion (Winter flower, Wheat leaf), Fujibakama (Ranso), Fujime bean (Tanto), grapes Fruit, peel, seeds, leaves ", beech, sycamore
(Cordyceps), Brazilian Licorice, Brazilian Carrot, Black Currant `` Fruit '', Blackberry, Bragnien, Plum `` Fruit '', Flucellaria, Blueberry
(Prunus americana), prune, floral blanka, blonde psyllium, bundo (green bean), hazelnut, luffa, safflower (safflower), henneken, belladonna, berry `` fruit '', persea, perbianberg, pereschia grandifolia, benceisou or ixa (Keiten), Boysenberry `` Fruits '', Butterflies or Elderweed or Nendowe or Haeukigi Kokia (Skin), Balsam (Fengsen, Acute child, Osteophyte), Potteria sapota, Potteria lukuma, Spinach, Bowfish ( Windbreak),
Spinach, physalis (Torone), honoki (Japanese thick,
Park), Bokeh (Gourd), Hosovanaokera (Soujutsu), Hosobanokirinsou (Kageten Sanshichi), Hosobabarengiku, Bodaiju
(Bodai tree), button (peony `` flower, leaf, stem, bark ''), buttonpi (peony bark), hops, jojoba, borage (ruriichisha), bordeaux, white berry `` fruit '', hon-oniku (meat ゅ ゅ, large Geisha), Hongsek-kok (iron bark stone, Sparrow mountain stone mackerel), Myzuruten Nansha (Tenan star), Maitake (maitake), ephedra (mao), maca, macadamia nut, margaret (mokushungiku), macri (seaweed), Magwa (Mulberry white bark "bark, leaves"), Magnolia splengeri, Masaranpouba, Masalan Zuba do Ceara, Masheira de de
Boi, Mashua (Tamanousenharen, Kyuukonkinrenka), Matabi (Kinten Tate), Matsukasa, Pine `` leaves, bark, roots '', Matta Oryo, Matsuhodo (Bukuryo), Matiko (Cordon Sillo), Mayorum (Hanahakka) , Malvanojazine (Bitterfruit), Mulberry (Fruit), Quince, Malein (Beloyd Mohsica), Mango, Mangosteen, Mansagna (Andesca Mitsure), Mansugurumi, Mandarin (Fruit), Mannentake (Lingzhi), Pheasant
(Kikomi `` fruits ''), Mishimasaiko (Saiko), Mizuobako or Mizuasahao (Ryugoshi), Mizokokushi (Harenbe Lotus), Mizohagi (Chisatsuna), Miyamanagi or Niyori willow (Henzo), Mitsugashiwa, Mitsuba, Midorisapote, Green peppermint, mimosa,
Ginger, miracle fruit `` fruit '', myrrh, mirobaran, wheat waragiku, mugwort (tree geun), mukunoki, mukuroji (extended life skin), munya, murasaki (purple root), murasakisikibu or omurasakishikibu (purple pearl), murasaki maize, murasaki natsufuji Kunming chicken blood wisteria), Megakanza opuntica, swordfish (meadow grass), scallop, melaleuca,
Melissa, melilot, melon `` fruit '', mugwort, moso bamboo, mok giraffe, moje (koboku), mokko (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), bean sprouts, morello cherry `` Fruits '', Moroheiya (burlap), Yakawamurasaki potato, yakuchi (masuchi), cornflower mackerel (cornflower), cornflower mint, yacon, yashabushi (arrow car) or Himeyashabushi or Obayashishabushi `` fruit, peel, fruit ear '', yachiyanagi, yatsude ( Octagon metal plate), mistletoe (willow parasitism), willow (river willow, tachiyanagi, weeping willow, red willow,
Cat willow, dog koreanagi, kinuyanagi, koreyanagi, unryuyanagi, yamayamayanagi, palm, yamayanagi, obanyanagi, shirokukinuyanagi, foxwillow, boninix), willow `` leaves, stems '', yabugarashi,
Yabu Koji (purple gold beef), Yabu cigarette (crane squirrel, Tenmeisei), pokeweed (commercial land), yamanoki (yamaharu), yamamomo (yang plum skin), yamamogi, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu "Fruit", lily, yogurusa, mugwort (Aiba), ligan (Rainuma), lime "fruit", rye, lakanka "fruit", raspberry "leaf,
Fruits, Latania (Crameria), Rakkyo or Shallot (Shiroi), Lavender, Longan (Longan flesh), Agave (Aono agave, Fukurin Agave), Ryokucha (green tea), Apple `` Fruits, seeds,
`` Leaves, roots '', gentian, rubus or suavisimus,
Ganoderma litchi (Lichi, Lichi nucleus), lettuce (Chisha), red currant `` fruit '', red pitaya, lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, wax palm, rokon (reed, radish), Loganberry "fruit", rosemary (mannen wax), rose hip
(Nobara), wasabi, warmoko (jiyu) and the like.

Other seawater, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango) Species, for example, sea mud or mud in various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients:
Silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese,
Arsenic, water), Shotoku stone.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia], and seaweed [Brown algae: Giant kelp (Macrocystis pilifera, Macrocystis.
Integrifolia, Neocitys luetokeana)], and seaweeds [Red algae: Tosakanori, Shikinori, Kainori, Yaleusbanori, Kagiusbanori, Sujiusbanori, Hyiusbanori, Akaminominori], and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Amidro spp.
Kingfisher, Marimo, Baronia: Tamago Baronia,
Tamabaronia, Magnuma: Magnificent, Magnificent: Ivy ivy, Rhododendron, Sericanthus, Hera Ivy, Chrysanthemum, Hanemomi, Mill Genus: Mill, Chromil, Sakibuchimil, Nagamil, Hiramil, Casanoli: Kasanori, Jizumomizumi, Genus Juzumozomi , Genus Colecaete, genus Cyperaceae, genus Raponica: Raspberry spp.
Hirohanohitoegusa, usuhitoegusa, motsukihitoe,
Genus Sylvestris, Ligustrum genus, Sumiremo: Sumiremo, Hoshimidoro, Fushinashimidoro, etc.).

Blue-green algae (Genus L .: L., L., L., L .: L., mushrooms,
(Hassay, Yuremo genus, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio) genus, etc.).

Brown algae (Piraella spp .: Piraella spp., Shiomidoro spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artaceae, Urabashiyahazu, Jigamigusa: Jingamigusa, Namimakura: Hirnamimakura,
Somenaceae: Somenaceae, Nabarimo: Nabarimo, Chasoumen: Motukichasomen, Matsumo:
Matsumo, Nagamatsumo: Genus Nagamo, Okinawamozuku: Okinawamozuku, Niseftomozuku: Niseftmozuku, Futomozuku: Futomozuku, Ishimozuku: Ishimozuku, Kuromo: Chromo, Nisemozuku: Nisemozuku,
Mozuku: Mozuku, Ishige: Ishige, Irolo, Ichimegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibosu, Urushigusa: Urushigusa, Keurushigusa, Tobacco, Kombomodo: Kombmodoki, Habanomogi , Genus Havanori: genus Habanori, Common Bukuro:
Common skull, Ezobukuro genus: Ezobukuro, Fukuronori genus: Fukuronori, Cottontail, Chishimafukuronori genus: Chishimafukuronori, Kagomenori genus: Kagomenori, Muralidri genus: Murachidori, Shamezugusa genus: Shamezugusa, Iwahige genus: Genus Lilium , Fennel: Fennel,
Genus Turmo: Genus Turmo, Aname: Aname, Stubble: Genus Limulus, Miss kelp: Miss kelp, Atsuba Miss kelp, Laminaria: Gatsugara kelp, Persimmon squam, Oni kelp, Gohei kelp, Naga kelp, Peas kelp,
Genus serrata, Trolocomb: Trolocomb, Antokume: Antokume, Scutellaria: Swordfish, Tularame, Kurome, Asterina: Asterine, Nesulecomb: Nesirecomb, Kuroshimea: Kuroshima, Nekoasicoma: Acerium, Araumenu Genus: Ainu-Wakame, Chigaiso, Oniwakame-ezoishige, Ezoishige, Yabanemoku: Yabamoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemok, Hondawara: Tamanashimoku, Isomok, Nagamomakumo, Nagamokumakumo , Nara samo, matamewara, tatsukuri, yasamata moku, umitoranoo, oobamoku, fushizushimoku, hahakimoku, togemoku, jolemoku, sawaw moku, obanoko Rimoku, Sugimoku genera: Sugimoku,
Genus genus: genus oleum, genus bulium: genus bulumium, genus cynomolgus: kayamoris, etc.).

Red algae (Russula genus: Rush genus, Funorinoshige, Amanori genus: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Isonohana: Nurakusa, Cronulla, Stinging genus, Hirakitoki:
Hirakintoki, Matabo genus: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakurate genus: Oobakintoki, Itofunori: Itofunori, Nagaobane: Nagaobane,
Genus Funori: Hanafunori, Fukurofunori, Makuhnori, Kalexigusa: Kalexigusa, Tosakamaki Genus: Hosobanotosakamaki, Hirohanokasakamuki, Yatsudegatatosakamoki, Kurosakamaki, Nezashinosakaeoka, Genus genus , Genus: Papilio californica, Giant porpoise, Papilio california, genus: Honoo, genus Lily:
Lycopodium, Usuginu, Asparagus genus: Astrophobicus, Aspergillus genus: Aspergillus genus, Aspergillus genus: Astrophobia, Okamuragusa genus: Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Rosa genus, Genus genus , Genus Ibaranori, genus Ibaranori, horned rose, Tachiibara, Kagiibaranori, genus genus: genus gibbon, genus Isodanu, genus Atsubanori: gen. Proboscis genus, Fushikenori: Fushikenori, Namiiwatake: Namiiwatake, Sponge genus: Scutellaria, Occidental genus: Ochinori, Sasa Mi genera: Itanigusa, rerun, Harigane, Hasujigusa genera: Hasujigusa, Suginori genera: Ikanoashi, Hosoibonori, Nobonori, Aqaba ginkgo Saw genera: Aqaba ginkgo Saw, Hishibukuro genera: Hishibukuro,
Madaragusa: Scutellaria, Amaranthus spp., Amaranthus genus: Amaranthus, Hanasakura, Apocynus: Amaranthus, Suzikonori;
Hirata goat: Hirata goat, Darus genus: Dars, Masagoshibari, Anadarus, Uebagsa: Uebagsa,
Genus Benifukuronori: Benifukukuronori, Fushiguni: Fushitsuguni, Himefushiguni, Hirohafushiguni, Watsunagisou: Hirawagatsugiso, Usubawagatsugiso, Aegis genus: Aegis, Keigis, Hariigis, Hanagigis,
Amixa, Egonori Genus: Egonori, Futogigi, Saeda Genus: Saeda, Chrysanthemum Genus: Chirimomiji, Konohanori Family: Habutaenori, Konohanori, Suzushironori, Usveni Genus: Usbeni, Genus Genus: Hashijinginu, Nagakonosuginogi, Genus Genus: The genus Hyusbanori: Kagiusbanori, Yaleusbanori, Sujiusbanori, Hyusbanori, Usubanolimodoki genus: Usvanorimodoki, Ayanishiki genus: Ayanishiki, Ayaginu genus: Ayaginu, Dadia genus: Enashidazia, Shimadagida, Genus: Genus genus Molloitus gutta, fustogusa, Macri genus: Macri, Salix genus: Hanayanagi, Yuna, Salix japonica, Motley Yuna, Beni Nagikori,
Mosayanagi, Sasabayanaginori, Sozogenus: Kurosozo, Kobusoso, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kanehagusa, Kozanemo: Kozanemo, Isomurasaki, Hosokonozemo, Himegokoke, Kurohimegoshio, Kurohimegoshigoshi : Isomegusa, Suginashigusa: Suginashigusa, Isobasho: Isobasho, Fujimatsumo: Fujimatsumo, Sawwort: Genus: Hawkins oak, Kawamozuku: Kawamozuku, Aokakawazuku, Himekawakuzuki, Genus Yugogomego Genus: genus, genus: genus etc.).

The axle algae (Charus spider, Shiratamamo,
Hossiturimo: Hossiturimo, Lychnotanus genus, Hessian moss: Himejirasamo, Chaboshiramo, Tripera, etc., and yellow algae (Hikarimo: Hikarimo etc.).

[0049] Materials derived from animal raw materials include a cockscomb extract, a bovine or human placenta extract, a pig or bovine stomach, duodenum or intestinal extract or a decomposition product thereof, and a pig or bovine spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Cow milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition products and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran (red bran, white bran) ferment extract, euglena extract, raw milk or skim milk powder Lactic acid fermentation products and trehalose or derivatives thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-
Aminobenzoic acid derivatives (such as paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), and methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, Octyl paramethoxycinnamate, paramethoxycinnamic acid
2-ethoxyethyl, sodium paramethoxycinnamate, potassium paramethoxycinnamate, monodiparamethoxycinnamate
Glyceryl-2-ethylhexanoate), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanin Acid derivatives (such as urocanic acid and ethyl urocanate), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, Nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, diamine Kishibenzon, Okutabenzon, sulisobenzone, benzo resorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, Neo Heliopan, ESKA roll,
Zinc oxide, talc, kaolin, etc.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and so on.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (such as magnesium phosphate of vitamin C and vitamin C glucoside), hydroquinone or a derivative thereof (such as hydroquinone benzyl ether), kojic acid or a derivative thereof, vitamin E or a derivative thereof Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, corn, waremokou, clara, mugwort, Honeysuckle, yellowfin, amberjack, matsuhodo, adlay, dragonflies, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamelis, yamaguwa, enmeisou, bellflower, toshishi, seizome, sun dried, mao, senkyu Dokkatsu, Psycho, storm, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether) and the like.

(21) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley).

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells,
Hypericum, Hamamelis, Clove, Melissa, Emmeso, Birch, Sage, Rosemary, Minami Amami,
Ages, ginkgo, green tea) etc.

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract and the like.

(27) Antibacterial / bactericidal / disinfectant Acrynol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde , Hexamine, brilliant green, malachite green,
Crystal Violet, Jamal, Photosensitizer 101
No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin and the like.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Natto extract, etc.

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, Mulberry leaves, Calyx, Gennoshoko, Comfrey, Sage, Sambucus nigra, Bodaiju, Buttonpipi) and the like.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (eg, estrone, estradiol, ethinylestradiol), isoflavones, oxendrone, etc.

(32) Peripheral blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, ginseng extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Stimulant Pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin, glyceride pentadecanoate and the like.

(35) Antiseborrheic agents Pyridoxine or its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agent Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guaiac wood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shape essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemon grass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, fresheners, stabilizers, emulsifiers, animal and plant proteins and their decomposed products, animal and plant Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents / antiallergic agents, cell activators, keratolytic agents, wound healing agents, foaming agents, thickening Agents, oral preparations, deodorants / deodorants, bittering agents, seasonings, enzymes, and the like can be used, and when used in combination therewith, various additive and synergistic effects can be expected.

Further, the dosage form of the elastase activity inhibitor or the cosmetic composition of the present invention is arbitrary, and capsules, powders, granules, solids, liquids, gels, air bubbles, emulsions, creams, ointments,
It can be used in the form of a sheet or the like.

Specifically, for example, basic preparations such as medicinal preparations for external use, lotions, emulsions, creams, ointments, lotions, oils, and packs, facial cleansers and skin cleansers, depilatory agents, depilatory agents, aftershave lotions , Pre-show lotion, shaving cream, foundation, lipstick, blusher, eye shadow, eyeliner, makeup cosmetics such as mascara, perfume, shampoo, rinse,
Hair treatments, pre-hair treatments, hair styling agents, perm agents, hair tonics, hair dyes, hair growth and hair restoration products, hair baths, deodorants, deodorants, cataplasms, plasters, tapes, sheets , Patches, aerosols, and other products such as toothpastes, mouth fresheners / gargles, sanitary cotton, wet tissues, etc.
In addition, it can be used for general foods and drinks.

The method of adding to the elastase activity inhibitor or the cosmetic composition of the present invention may be added in advance or during the production, and may be appropriately selected in consideration of workability. good.

[0083]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) Sea turtle, kelp, hijiki, hibamata, wakame, giraffe, kotoji nonomata,
100 g of dried seaweed such as cucumber, liquorice, tengusa, aonori and anaoasa were heated and extracted with purified water (about 80 ° C.) for about 5 hours, filtered and the extract (dry solids: about 0.5 to 3.0)
%) Of about 1.0-3.0 kg.

(Production Example 2) Sea prickly pear, kelp, hijiki, hibamata, wakame, giraffe, kotoji nonomata,
100 g of dried seaweeds of cucumber, liquorice, promenaceae, acrophora, aonori and anaaosa are immersed in a 50% ethanol solution.
After extraction at room temperature for 5 days and nights, filtration is performed to obtain about 1.0 to 3.0 kg of an extract (dry solid content: about 0.6 to 2.0%).

(Production Example 3) Sea prickly pear, kelp, hijiki, hibamata, wakame, giraffe, kotoji nonomata,
100 g of dried seaweed such as cucumber, limen, tengusa, aonori and anaoasa were gently heated and extracted at 50 ° C. for 6 hours at 50 ° C. with 1 L of an equal mixture of 30% propylene glycol solution and 30% 1,3-butylene glycol solution. Suction filtration is performed to obtain about 1.0 to 3.0 kg of an extract (dry solid content: 0.8 to 2.5%).

(Test 1) Elastase Activity Inhibitory Activity Test "Test Method and Evaluation Method"
Evaluation was performed using succinyl-ala-ala-ala-p-nitroanilde. The substrate was adjusted to 0.1 M with dimethyl sulfoxide, and a 1 mM solution diluted 100-fold with a buffer at the time of use was used. The enzyme was adjusted to 0.05 unit / mL with a buffer. 100 μL of substrate was reacted with 50 μL of enzyme and 50 μL of inhibitor at 37 ° C. for 30 minutes, and then 405 nm
The absorbance was measured by using the above formula, and the inhibition rate was calculated by the following equation 1. The buffer used was a 0.2 M Tris-HCl buffer (pH 8.8). The results are shown in FIGS. a. Sample The sample for the elastase activity inhibitory test was the extract obtained in Production Example 1 of the present invention (adjusted to a solid concentration of 1.0%).
Was used for the test. As comparative samples, dipotassium glycyrrhizinate and brown algae (Matsumo:
Matsumo), red algae (Bovine genus: Bovine genus, Akaba genus: Akaba), and green algae (Volvox genus: Volvox) were prepared under the same conditions as the extracts obtained by the same production method as in Production Example 1 and used for the test. Was.

<Equation 1> Inhibition rate (%) = 100− (presence of inhibitor / no inhibitor) × 100

(Test Results) As shown in FIG. 1, it was confirmed that the seaweed (brown algae) extract of sea urchin, kelp, hijiki, hibamata and wakame of the present invention had an effect of significantly suppressing elastase activity. In addition, dipotassium glycyrrhizinate and the brown algae, Pinegrass, did not show an elastase activity inhibitory effect. Next, as shown in FIG. 2, it was confirmed that the seaweed (red algae) extract of the ginseng, kotojinomatata, sugino-ri, limen, and tengusa of the present invention also had an effect of significantly suppressing elastase activity.
Similarly, dipotassium glycyrrhizinate and red algae Aqaba did not show an elastase activity inhibitory effect. Furthermore, as shown in FIG. 3, it was confirmed that the seaweed (green algae) extract of Aonori and Anaoasa of the present invention also had an effect of significantly suppressing elastase activity. Similarly, dipotassium glycyrrhizinate and volvox of green algae did not show an elastase activity inhibitory effect.

(Test 2) Safety test (1) Primary skin irritation test Each of the seaweed extracts obtained in Production Examples 1 to 3 had a solid concentration of about
It was adjusted to 2.0%, and this was applied to the skin of a Japanese white rabbit (female, 3 birds per group, weighing about 2.3 kg) whose back was shaved.
0.03 mL was applied per square of × 2 cm ² . Judgment was carried out 24, 48, and 72 hours after application using the primary irritation scoring method with erythema and edema as indices. As a result, in all the animals, no erythema and edema were observed, and the animals were determined to be negative.

(Test 3) Safety Test (2) Skin Cumulative Irritation Test Each of the seaweed extracts obtained in Production Examples 1 to 3 had a solid concentration of about
Prepared in 2.0% and so as purified water, which flank shaved (2 × 4 cm ²⁾ was Hartley guinea pigs (female,
Once a day, 5 times a week on the skin of 3 animals per group, weight around 320g)
0.5 mL / animal was applied. Application is for 2 weeks
Shaving was performed on the last application day of each week. The determination was made on the day following each application day and on the day after the last application day, using primary irritancy scoring as an index of erythema and edema. As a result, in all animals, no erythema and edema were observed for 2 weeks after application, and the animals were judged to be negative.

(Test 4) Safety test (3) Single dose toxicity test Each seaweed extract powder obtained in Production Examples 1 to 3 was adjusted with purified water to a concentration of 12.5%. kg quantity,
Before the test, ddy mice (male and female, 5 animals per group, 5 weeks old), which had been fasted for 16 hours, were orally administered with a sonde, and the appearance and extent of toxic symptoms were observed over time. As a result, no abnormality was observed in all the mice for 14 days, and no abnormalities were found in the dissection results. Therefore, LD50 is 2,00
It was 0 mg / kg or more, so it was judged as a safe substance.

(Formulation Examples) Production of Elastase Activity Inhibitor or Cosmetic Composition The following formulation examples are shown in accordance with the above evaluation results, and each formulation example may be one produced by a conventional method in the production of each product. , Only the blending amount is shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7.1,3-butylene glycol 1.0 8. Glycerin 2.0 9. A: Apricot extract of Production Example 1 B: Kombu extract of Production Example 1 C: Hijiki extract of Production Example 1 D: Production Hibamata extract of Example 1 E: Wakame extract of Production Example 1 F: Giraffe extract of Production Example 1 G: Kotoji nonomata extract of Production Example 1 H: Suginori extract of Production Example 1 I: Hymen extract of Production Example 1 Liquid J: Extract of Aspergillus oryzae of Production Example 1 K: Extract of Aonori of Production Example 1 L: Extract of Anaphora of Production Example 1 * Extract of any one of A to L 5.0 10. Preservative (paraoxybenzoate) ) Appropriate amount 11.Purified water 100 residue

(Formulation Example 2) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Glyceryl paraaminobenzoate 1.0 4. Ethyl urocanate 1.0 5. Magnesium salt of vitamin C phosphate ester 1.0 6. Cetyl alcohol 1.5 7. Glycerin monostearate 2.0 8. Polyoxyethylene (20) cetyl ether 3.0 9. Polyoxyethylene (20) soorbitan monooleate 2.0 10.1,3-butylene glycol 1.0 11. Glycerin 2.0 12. Kombu extract of Production Example 1 3.0 13. Suginori extract of Production Example 1 3.0 14. Preservative (paraoxybenzoate) qs 15. Purified water 100 residue

(Formulation Example 3) Cosmetic oil wt% 1. Liquid paraffin 30.0 2. Squalane 20.0 3. Olive oil 20.0 4. Isopropyl palmitate 10.0 5. Sea urchin hot water extract 2.0 6. Tengusa hot water extract 2.0 7. Aonori hot water extract 2.0 8. Grapefruit fruit or leaf hot water extract 1.0 9. Olive oil 1.0 10. Shea fat 1.0 11. Butylhydroxyanisole 0.1 12. Vitamin E derivative 0.1 13. Preservative (benzalkonium chloride) qs 14 .Perfume (Musk water) Appropriate amount 15. Residual water with 100 purified water

(Formulation Example 4) Soft lotion weight% 1. Glycerin 5.0 2.1,3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. 50% ethanol extraction with kelp Liquid 1.0 6. 50% ethanol extract of Kotoji no nomata 1.0 7. 50% ethanol extract of dimentia 1.0 8. 50% ethanol extract of laurel leaves 1.0 9. 50% ethanol extract of leaves or stem of Atlantic vegetation 1.0 10. Antibacterial and preservative (Lactoferrin solution) Appropriate amount 11. Fragrance (Western thin water) Appropriate amount 12. Purified water 100 residue

(Formulation Example 5) Astringent lotion weight% 1. sorbitol 2.0 2. POE (20) oleyl alcohol 1.0 3. ethanol 15.0 4. kelp 50% 1,3-butylene glycol extract 2.0 5. polyester 50% 1 2,3-butylene glycol extract 2.0 6. Hibamata 50% 1,3-butylene glycol extract 2.0 7. Suginori 50% 1,3-butylene glycol extract 2.0 8. Tengusa 50% 1,3-butylene glycol extract 2.0 9. Green tea leaf or stem 50% 1,3-butylene glycol extract 1.0 10. Hamamelis leaf or bark 50% 1,3-butylene glycol extract 1.0 11. Allantoin 0.2 12. Astringent (zinc sulfocarbonate) 0.2 13. Antibacterial and preservative (thiamine lauryl sulfate) appropriate amount 14.Perfume (lavender water) appropriate amount 15.Purified water 100 residue

(Formulation Example 6) Two-layer lotion (liquid to liquid type) weight% 1. squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. sorbit 1.0 4. ethanol 15.0 5. kelp 30% ethanol extract 2.0 6. Ginseng rhinoceros 30% ethanol extract 2.0 7. Anaosa 30% ethanol extract 2.0 8. Sensoji rhizome 30% ethanol extract 2.0 9. Sage whole plant 30% ethanol extract 2.0 10. Coltsfoot flower and leaf 30% ethanol extract Liquid 1.0 11. Pomelo fruit 30% ethanol extract 1.0 12. Ascorbic acid 0.2 13. Astringent (calamine) 0.1 14. Preservative (phenoxyethanol) qs 15. Fragrance (citrus water) qs 16. Purified water 100

(Formulation Example 7) Carmine lotion (liquid to solid)% by weight 1. Glycerin 3.0 2.1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Sea urchin 50% propylene glycol extract 1.0 8. Dimentia 50% propylene glycol extract 1.0 9. Ginger fruit 50% propylene glycol extract 1.0 10. Hypericum flower or leaf 50% propylene glycol extract 1.0 11. Placenta extract 1.0 12 .Camphor 0.2 13. Phenol 0.03 14. Preservative (photosensitive element 201 & 401,) suitable amount 15. Fragrance (Ogon water) proper amount 16. Purified water 100 residue

(Formulation Example 8) Pack weight% 1. Polyvinyl alcohol 15.0 2. Carboxymethylcellulose sodium 5.0 3. Propylene glycol 3.0 4. Ethanol 10.0 5. Aonori 50% ethanol extract 1.0 6. Anaoa 50% ethanol extract 1.0 7 .50% ethanol extract from Kotoji no nomata 1.0 8. 50% ethanol extract from cherry blossom leaves 1.0 9. 50% ethanol extract from peach leaves 1.0 10. 50% ethanol extract from rhizome rhizome 1.0 11. Preservative (paraoxybenzoate) qs 12. flavor (Tokimizu) Appropriate quantity 13. Residual water with 100 purified water

(Formulation Example 9) Peel-off pack weight% 1. Glycerin 6.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 10.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Anaoasa hot water extraction Liquid 3.0 8. Eggfish 30% ethanol extract 1.0 9. Pepper 30% ethanol extract 1.0 10. Hosobabarengiku (Echinashi) leaf 30% ethanol extract 1.0 11. Arnica flower or leaf 30% ethanol extract 1.0 12. Preservative ( Paraoxybenzoic acid ester) Appropriate amount 13.Fragrance (Hiba leaf distilled water) Appropriate amount 14.Purified water 100 residue

(Formulation Example 10) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 5.0 5. Chamomile oil 5.0 6. Palm oil 5.0 7. Borax 0.5 8. Kotoji no nomata extract 2.0 (Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 9.Oat bark extract 2.0 (Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 10.Birch bark extract 2.0 (Ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 11. Comfrey leaf extract 2.0 (ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 12. Liquid shea fat 1.0 13. Preservative (acrinol) 0.1 14.Perfume (sage water) Appropriate amount 15.Purified water 100 residue

(Formulation Example 11) Emollient cream% by weight 1. Stearic acid 2.0 2. Stearyl alcohol 7.0 3. Reduced lanolin 2.0 4. Squalane 5.0 5. Octyldodecanol 6.0 6. Polyoxyethylene cetyl ether (25E.O.) 3.0 7. Lipophilic glyceryl monostearate 2.0 8. Propylene glycol 5.0 9. Anaaosa 50% 1,3-butylene glycol extract 3.0 10. Chimney peel 50% 1,3-butylene glycol extract 3.0 11. Pine fruit (pine cone) ) 50% 1,3-butylene glycol extract 3.0 12. Orange peel 50% 1,3-butylene glycol extract 3.0 13. Preservative (paraben) suitable amount 14. Fragrance (cinch water) proper amount 15. Purified water 100 Residue

(Formulation Example 12) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Aonori extraction Liquid 2.0 (Ethanol: 1,3-butylene glycol = 1: 2 extraction solvent) 8. Wakame extract 2.0 (Ethanol: 1,3-butylene glycol = 1: 2 extraction solvent) 9. Raspberry fruit 50% 1,3- Butylene glycol extract 2.0 10. Loquat leaf 50% 1,3-butylene glycol extract 2.0 11. Western elderberry flower or berry 50% 1,3-butylene glycol extract 2.0 12. Chamomile flower 50% 1,3-butylene Glycol extract 2.0 13. carrageenan 1.0 14. dipotassium glycyrrhetinate 1.0 15. apricot kernel particles 0.1 16. preservative (salicylic acid) 0.1 17. perfume (sage water) suitable amount 18. purified water 100 residue

(Formulation Example 13) Face Wash Cream% by Weight 1. Stearic acid 10.0 2. Palmitic acid 10.0 3. Myristic acid 12.0 4. Lauric acid 4.0 5. Oleyl alcohol 1.5 6. Lanolin derivative (EO adduct) 1.0 7. Glycerin 18.0 8. Potassium hydroxide 6.0 9. A: Sea urchin extract of Production Example 2 B: Kombu extract of Production Example 2 C: Hijiki extract of Production Example 2 D: Hibamata extract of Production Example 2 E: Production Example 2 W: seaweed extract F: ginseng extract of production example 2 G: kotojinomatsuta extract of production example 2 H: cinnamon extract of production example 2 I: pirimen extract of production example 2 J: extract of squirrel of production example 2 K ： Aonori extract of Production Example 2 L ： Anaosa extract of Production Example 2 ※ Extract of any one of A to L 5.0 10. Royal jelly extract 1.0 (Ethanol: 1,3-butylene glycol = 1: 1 extract 11.Glycyrrille Phosphoric acid 1.0 12. Preservative (salicylic acid) 0.1 13. Appropriate amount of fragrance (Tokimizu) 14. Purified water 100 residue

(Formulation Example 14) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Whey 1.5 5. Kirinsai 70% 1,3-butylene glycol extract 0.5 6. Polymen 70% 1,3-butylene glycol extract 0.5 7. Wakame 70% 1,3-butylene glycol extract 0.5 8. Loquat leaf 70% 1,3-butylene glycol extract 0.5 9. Kiwi fruit 70% 1,3 -Butylene glycol extract 0.5 10. Panax ginseng root 70% 1,3-butylene glycol extract 0.5 11. Polyethylene powder 0.5 12. Hydroxypropyl chitosan solution 0.5 13. Amino acids (glycine, glutamine, etc.) 0.5 14. Preservative ( Undecylenic acid, phenol) Appropriate amount 15. pH adjuster Appropriate amount 16. Perfume (shower water) Appropriate amount 17. Refined water 100

(Formulation Example 15) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Polyethyl acrylate 1.0 9.1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 1.0 12. Titanium dioxide 5.0 13. Suginori 50% dipropylene glycol extract 0.5 14. Kotoji no matata dipropylene glycol extract 0.5 15. Peony skin 50% dipropylene glycol extract 0.5 16. Birch bark 50% dipropylene glycol extract 0.5 17. Hypericum whole plant 50% dipropylene glycol extract 0.5 18. Saiko root 50% dipropylene glycol extract 0.5 19. Preservative (paraben) qs 20. Fragrance (apple water) qs 21. Purified water 100 and the rest

(Formulation Example 16) Sunscreen cosmetics (oil type)% by weight 1. Liquid paraffin 70.0 2. Ethyl paraaminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Hibamata aqueous extract 1.0 6 Hijiki water extract 1.0 7. Sancha flower whole plant water extract 0.5 8. Shakuyaku root or leaf water extract 0.5 9. Ginger rhizome water extract 0.5 10. Tachisou whole plant water extract 0.5 11. Comfrey water extract 0.5 12. Dihydroxymethoxybenzophenone 0.2 13. Antioxidant (butylhydroxytoluene) proper amount 14. Fragrance (jasmine water) proper amount

(Formulation Example 17) Cream foundation (O / W type) wt% 1. stearic acid 4.0 2. glyceryl monostearate 3.0 3. cetanol 1.5 4. isopropyl myristate 7.0 5. liquid paraffin 10.0 6. salami beeswax 3.0 7. Triethanolamine 1.5 8. Glycerin 3.0 9. Bentonite 1.0 10. Titanium oxide 8.0 11.2,4-Dihydroxybenzophenone 8.0 12. Kaolin 3.0 13. Talc 3.0 14. Color pigment (iron iron oxide) 1.0 15. Kombu 70% ethylene Glycol-extracted dry powder 0.2 16. Centrifugal leaf or stem 70% ethylene glycol-extracted dry powder 0.2 17. Mulberry leaf 70% ethylene glycol-extracted dry powder 0.2 18. Preservative (paraben) qs 19. Fragrance (sandalwood water) qs 20. Purification Water 100 and the rest

(Formulation Example 18) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. 30% 1,2-butylene glycol extract in sea urchin 2.0 2.0 7. Usberia oyster leaf 30% 1,2-butylene glycol extract 2.0 8. Mulberry bark 30% 1,2-butylene glycol extract 2.0 9. Kuzukane 30% 1,2-butylene glycol extract 1.0 10. Hydroxypropyl chitosan solution 2.0 11. Mucopolysaccharide solution 1.0 12. Appropriate amount of antibacterial and preservative (Photosensitizer No. 101) 13. Appropriate amount of pH adjuster 14. Appropriate amount of perfume (hamamelis water) 15. Residue with 100 purified water

(Formulation Example 19) Rinse weight% 1. Stearyl trimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Kotoji nonomata 50% 2,3-butylene glycol extract 1.0 6. 2,3-butylene glycol extract from 50% chamomile flower or stem 1.0 7. 50% 2,3-butylene glycol extract from Soapwort leaf or stem 1.0 8. 50% 2,3-butylene glycol extract from Astragalus leaf 9. Hydroxyethyl chitosan solution 1.0 10. Trehalose solution 1.0 11. Hydrolyzed silk solution 1.0 12. Antibacterial and preservative (benzalkonium chloride) qs 13. perfume (lavender water) qs 14. Purified water 100

(Formulation Example 20) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Ethylene glycol 3.0 3.0 6. Wakame 50% ethylene glycol Extract 1.0 7. Forsythia fruit 50% ethylene glycol extract 1.0 8. Strawberry fruit 50% ethylene glycol extract 1.0 9. Trehalose solution 1.0 10. Hydrolyzed keratin solution 1.0 11. Antibacterial and preservative (benzalkonium chloride) 12. Appropriate amount of fragrance (chamois water) 13. Residual water with 100 purified water

(Formulation Example 21) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Suginori hot water extract 2.0 6. Umiuchiwa Hot water extract 2.0 7. Apricot fruit or leaf hot water extract 1.0 8. Hot spice (silicone spicy) whole plant hot water extract 1.0 9. Ginkgo biloba hot water extract 1.0 10. Carpronium chloride 0.3 11. Hinokitiol 0.5 12. Keratin decomposition solution 1.0 13. Antibacterial and preservative (paraben, benzalkonium chloride) proper amount 14. Fragrance (rose water) proper amount 15. Purified water 100 residue

(Formulation Example 22) Hair liquid% by weight 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Trimethylene glycol 5.0 6. Tengusa 50% Propylene glycol extract 1.0 7. Euglena root or rhizome 50% ethanol extract 1.0 8. Mugwort whole plant 50% ethanol extract 1.0 9. Oolong tea leaf or seed 50% ethanol extract 1.0 10. Azulene 1.0 11. Antibacterial and preservative (Paraben, benzalkonium chloride) Appropriate amount 12. Fragrance (Western light water) Appropriate amount 13. Purified water 100 residue

(Formulation Example 23) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. A: Apricot extract of Production Example 1 B: Production Example 1 C: Extract of the kelp extract of Preparation Example 1 D: Extract of Hibikita of Preparation Example 1 D: Extract of Hibamata of Preparation Example 1 E: Extract of Wakame seaweed of Preparation Example 1 F: Giraffe extract of Preparation Example 1 G: Extract of Kotoji no Nomata of Preparation Example 1 H : Suginori extract of Production Example 1 I: Hypermeni extract of Production Example 1 J: Extract of Astragalus extract of Production Example 1 K: Aonori extract of Production Example 1 L: Anaoasa extract of Production Example 1 * Any of A to L One kind of extract 5.0 5. Peony skin 60% ethanol extract 3.0 6. Citrus leaves or fruit 60% ethanol extract 3.0 7. Preservative (paraben) qs 8. Purified water 100 residue

(Formulation Example 24) Hair tonic% by weight 1. 1-Menthol 0.2 2. Ethanol 32.5 3. Polyoxyethylene hydrogenated castor oil 0.5 4. Propylene glycol 5.0 5. Diphenhydramine hydrochloride 0.3 6. Polymen hot water extract 2.0 7 Hot water extract of Arkan fruit fruit 1.0 8. Hot water extract of spinach rhizome 1.0 9. Hot water extract of corn whole plant 1.0 10. Chicken cop extract 1.0 11. Carboxymethyl chitin solution 1.0 12. Vitamin E derivative 0.5 13. Antibacterial Preservative (paraben) 0.1 14.Perfume (cornflower water) suitable amount 15.Purified water 100 residue

(Formulation Example 25) Hair restorer / hair restorer wt% 1. Ethanol 60.0 2. Hibamata 50% propylene glycol extract 5.0 3. Ginseng whole plant 50% propylene glycol extract 2.0 4. Green tea (leaf or seed) 50% Propylene glycol extract 2.0 5. Yarrow fruit 50% propylene glycol extract 1.0 6. Vitamin E derivative 0.5 7. Pepper tincture 0.5 8. Resorcin 0.5 0.5 Glycyrrhizic acid 0.5 10. Hydroxypropyl chitosan solution 0.5 11. Beef hematin solution 0.5 12. Mucin solution 0.5 13. Brown sugar extract from sugarcane 0.5 14. Amino acids (glycine, glutamine, etc.) 0.5 15. Antibacterial and preservative (paraben) 0.1 16. Perfume (rosemary water) Appropriate amount 17. Make purified water 100 Residue

(Formulation Example 26) Hair restorer / hair restorer weight% 1. Ethanol 60.0 2. Polymen 50% 1,3-butylene glycol extract 1.0 3. Kotoji no nomata 50% 1,3-butylene glycol extract 1.0 4. Fruit 50% 1,3-butylene glycol extract 1.0 5.Bukuryo bacteria nucleus 50% 1,3-butylene glycol extract 1.0 6. Loofah whole plant 50% 1,3-butylene glycol extract 0.5 7. Canthalis tincture 0.5 8. Pantothenic acid derivative 0.5 9. Resorcin 0.5 0.5 Glycyrrhizic acid 0.5 11. Salicylic acid 0.5 12 Hydroxyethylchitosan solution 0.5 13. Elastin hydrolyzate 0.5 14. Antibacterial and preservative (paraben) 0.1 15. Perfume (lavender water) 16. Residue with 100 purified water

(Formulation Example 27) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 2.0 4. Hibamata hot water extraction dry powder 5.0 5. Suginori hot water extraction dry powder 5.0 6. Ao-oli hot water extraction dry powder 5.0 7. Western thin leaf or stem hot water extraction dry powder 5.0 8. Saxifraga whole plant hot water extraction dry powder 5.0 9. Pigment (Gunjo green) qs

(Formulation Example 28) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 4.0 4. Egg extract 70% ethanol extracted dry powder 1.0 5. Cornflower flower or leaf stem 70% ethanol Extract dried powder 1.0 6. Summer orange fruit or peel 70% ethanol extracted dry powder 1.0 7. Rose 70% ethanol extracted dry powder 1.0 8. Aloe pulp 70% ethanol extracted dry powder 1.0 9. Apple juice powder 1.0 10. Pigment ( Gunjo, chromium hydroxide) Suitable amount 11. Fragrance (hamamelis water) Suitable amount

(Formulation Example 29) Coating protective agent A gauze or liniment cloth is impregnated with a prescription solution obtained by mixing an appropriate amount of hot kelp extract, antibiotics, anti-inflammation, etc., and attached to the wound. Alternatively, the Aonori or sea urchin extract may be directly applied locally and covered with gauze or the like.

(Test 5) Use Effect Test The effect of the cosmetic composition of the present invention actually used was examined. The test was performed using 20 female adults aged 30 to 50 years old who suffer from fine wrinkles or shiny / skin-free skin as panelists. This was done by applying over a period of months. As a control, a preparation prepared by removing a plant extract of the present invention from the emulsion in the same manner was used.
The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numbers in the table represent the number of persons. No one complained of skin abnormalities during the use period.

"Wrinkle and fine wrinkle improvement effect" Efficacy: Wrinkles and fine wrinkles became less noticeable. Slightly effective: wrinkles and small wrinkles are slightly less noticeable. Ineffective: No change from before use.

"Effect of improving skin gloss and firmness" Effective: Skin gloss and firmness increased. Slightly effective: Gloss and firmness of skin increased slightly. Ineffective: No change from before use.

[0126]

(Test results) As shown in Table 1, seaweed (brown algae) extract of sea urchin, kombu, hijiki, hibamata and wakame of the present invention, giraffe, kotoji nonomata, suginori,
Emulsion containing seaweed (red algae) extract of Hirimen and Tengusa, and seaweed (green algae) extract of Aonori and Ana-aosa significantly imparts gloss to skin and reduces wrinkles and fine wrinkles. did it.

[0128]

EFFECT OF THE INVENTION The brown algae of the present invention (Umiuchiwa, Kombu,
An elastase activity inhibitor or a cosmetic composition containing at least one seaweed extract selected from among the following: It has been confirmed that it has an elastase activity inhibitory effect, it gives the skin a gloss and shine and can prevent aging, and also reduces wrinkles and fine wrinkles and maintains a youthful skin condition You can also. The present invention can be used for other general foods and drinks.

[Brief description of the drawings]

FIG. 1 is a graph showing the elastase activity inhibition rate of each seaweed extract of the brown algae (Sea urchin, Kombu, Hijiki, Hibamata, Wakame) of the present invention.

FIG. 2 is a graph showing the elastase activity inhibition rate of each seaweed extract of the red algae (Kirinsai, Kotojutsu nomata, Suginori, Hirimen, Tengusa) of the present invention.

FIG. 3 is a graph showing the elastase activity inhibition rate of each seaweed extract of green algae (Aonori, Anaoasa) of the present invention.

────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] February 22, 2001 (2001.2.2)
2)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0025

[Correction method] Change

[Correction contents]

The elastase activity inhibitor or the cosmetic composition of the present invention may optionally contain the following components and additives as required, in addition to the above-mentioned essential components, as long as the effects of the present invention are not impaired. Can be selected and used together,
The content in the formulation is not particularly specified, but usually 0.0001
~ 50% is preferred.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61K 7/06 A61K 7/06 7/075 7/075 7/08 7/08 7/42 7/42 7 / 50 7/50 35/80 35/80 Z A61P 17/00 A61P 17/00 // A61K 7/02 A61K 7/02 A F term (reference) 4C083 AA072 AA082 AA111 AA112 AA122 AB032 AB152 AB212 AB232 AB242 AB312 AB352 AB442 AC012 AC022 AC072 AC092 AC102 AC112 AC122 AC132 AC152 AC182 AC212 AC242 AC342 AC352 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC552 AC582 AC642 AC682 AC692 AC772 AC792 AC852 AC862 AC902 AD092 AD112 AD212 AD272 AD322 AD352 AD412 AD442 AD452 AD512 CC532 AD532 CC552 CC64662 CC12 CC19 CC23 CC25 CC32 CC37 CC38 CC39 DD05 DD16 DD23 DD30 DD31 DD33 EE12 EE17 EE22 FF01 4C088 AA13 AA14 AA15 AC05 BA09 BA10 CA03 MA63 ZA89 ZC20

Claims (3)

[Claims] An elastase activity inhibitor comprising at least one seaweed extract selected from brown algae, red algae and green algae. 2. One or more seaweeds selected from seaweed, kelp, hijiki, hibamata, wakame and red algae among brown algae; An elastase activity inhibitor comprising an extract. 3. A cosmetic composition comprising the elastase activity inhibitor according to claim 1 or 2.