CN212655788U

CN212655788U - Movable pathogen nucleic acid detection laboratory system supporting rapid deployment

Info

Publication number: CN212655788U
Application number: CN202020964583.0U
Authority: CN
Inventors: 谢孝民; 李丹; 熊磊; 谢正华; 陈才夫; 袁庆明; 王兆松; 张立振
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2020-05-29
Filing date: 2020-05-29
Publication date: 2021-03-05
Anticipated expiration: 2030-05-29

Abstract

The utility model relates to a support portable pathogen nucleic acid testing laboratory system of quick deployment, through storing and preparing the reagent that the nucleic acid testing process relates to, sample preparation, the setting that the three main step of amplification and result analysis is independent respectively is in three movable structure, realize the isolation requirement between the three step in the nucleic acid testing process, three movable structure can quick travel deploy to arbitrary demand position and expand and form a pathogen nucleic acid testing laboratory that has complete function simultaneously, realize that key prevention and control position carries out the detection on the spot, avoid high risk group to concentrate the transfer.

Description

Movable pathogen nucleic acid detection laboratory system supporting rapid deployment

Technical Field

The utility model relates to a pathogen detection equipment technical field especially relates to a support portable pathogen nucleic acid testing laboratory system of quick deployment.

Background

The most commonly used nucleic acid detection method at present is a screening means with high public accuracy and reliable result, but the nucleic acid detection generally needs to be operated in a detection laboratory meeting the requirements, so that the number of the detection laboratories is not enough to meet huge detection requirements, the nucleic acid detection efficiency is severely restricted, and the optimal treatment time is delayed in the process of waiting for the nucleic acid detection by a plurality of patients, so that the disease condition is aggravated.

On the other hand, the current method of collecting samples in a fixed-point hospital and then sending the samples to a detection laboratory in a centralized manner is mostly adopted for nucleic acid detection, so that a large number of patients gather in the fixed-point hospital, the risk of cross infection is increased, and the possibility of pollution of a nucleic acid detection reagent is increased, so that misdiagnosis is caused; for some key prevention and control positions, such as airports, railway stations and other places with dense people flow, people are necessarily concentrated by adopting a people flow concentrated transportation detection method, pathogen infection risks are increased, and pathogen prevention and control are not facilitated.

SUMMERY OF THE UTILITY MODEL

For solving prior art's not enough, the utility model provides a support portable pathogen nucleic acid testing laboratory system of quick deployment, through the reagent preparation that involves with the nucleic acid testing process, sample treatment, the three main step of sample analysis independent setting respectively is in three movable structure, realize the isolation requirement between the nucleic acid testing process to three step, three movable structure can quick travel deploy to arbitrary demand position and expand and form a pathogen nucleic acid testing laboratory that has complete function simultaneously, realize that key prevention and control position carries out the detection on the spot, avoid high risk group to concentrate the transfer.

In order to achieve the above object, the utility model discloses the technical scheme who adopts includes:

a mobile pathogen nucleic acid detection laboratory system supporting rapid deployment comprising reagent storage and preparation mobile structures, specimen preparation mobile structures and amplification and product analysis mobile structures isolated from each other;

the reagent storage and preparation mobile structure comprises a reagent dispensing module maintaining a positive pressure environment, at least one airtight transfer window disposed in a sidewall of the reagent dispensing module, and a first buffer module connecting an external environment with the reagent dispensing module to form an airtight personnel access; the reagent preparation module is provided with a nucleic acid detection reagent preparation component, a nucleic acid detection reagent subpackaging component and a nucleic acid detection reagent storage component;

the specimen preparation movable structure comprises a nucleic acid processing module maintaining a negative pressure environment, at least one airtight transmission window arranged on the side wall of the nucleic acid processing module, and a second buffer module connecting an external environment and the nucleic acid processing module to form an airtight personnel channel; the nucleic acid processing module is provided with a sample inactivation assembly, a sample nucleic acid extraction assembly, a nucleic acid sample adding assembly and a to-be-detected reagent sealing assembly;

the movable amplification and product analysis structure comprises a nucleic acid amplification and data analysis module maintaining a negative pressure environment, at least one airtight transmission window arranged on the side wall of the nucleic acid amplification and data analysis module, and a third buffer module connecting the external environment and the nucleic acid amplification and data analysis module to form an airtight personnel channel.

Further, the specimen preparation mobile structure and/or the amplification and analysis mobile structure further comprises a separate hermetically isolated waste disposal device.

Further, positive pressure environment is maintained to first buffer module, second buffer module and third buffer module, just the positive pressure ambient pressure value of first buffer module is higher than reagent preparation module positive pressure ambient pressure value.

Further, the specimen preparation movable structure further comprises an external sample collection device which can be unfolded/folded; at least one airtight transfer window is arranged between the external sample collection device and the nucleic acid processing module.

Further, the reagent storage and preparation movable structure, the specimen preparation movable structure and the amplification and product analysis movable structure are respectively connected with a mains supply through detachable cables for power supply.

Further, the amplification and product analysis moveable structure further comprises a power supply component; the power supply assembly connects and provides electrical power to the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and/or the amplification and product analysis mobile structure via detachable cables.

Further, the power supply assembly is movably accommodated in the third buffer module and moves out of the third buffer module to re-determine the placement position when power is supplied, so that the length of any detachable cable connected with the power supply assembly is less than or equal to 10 meters.

Further, the movable structure is provided with at least one sunshade capable of being unfolded/folded in at least one direction around the movable structure; the unfolding width of the sunshade is not less than 3 m.

Further, the reagent storage and preparation movable structure, the specimen preparation movable structure and the amplification and product analysis movable structure are in a static placing position with a linear distance not less than the unfolding width of the sunshade.

Further, the movable structure comprises a van, a box type trailer or a container with an airtight isolated cab, and any other movable vehicles capable of meeting the requirements.

The utility model has the advantages that:

adopt support portable pathogen nucleic acid detection laboratory system of quick deployment, bear respectively the different steps of carrying out among the nucleic acid testing process through the movable structure who adopts three mutually independent isolation, can the different operational environment of adjustment of pertinence: for the reagent preparation step with high requirement on environment cleanliness, a positive pressure environment is adopted to ensure that the internal air is not polluted by the outside; for the sample processing step involving pathogen contact, a negative pressure environment is adopted to prevent the pathogen from accidentally leaking, ensure safety and prevent secondary transmission. Meanwhile, the nucleic acid detection laboratory is divided into three mutually independent parts, the three parts are randomly placed and arranged according to conditions, different site conditions can be better adapted, the mobile detection laboratory can be conveniently unfolded and arranged, and the nucleic acid detection work can be carried out in a limited space. By applying the mobile pathogen nucleic acid detection laboratory system supporting rapid deployment, the nucleic acid detection capability can be rapidly mobilized to a hotspot demand area, the detection capability is rapidly formed, time-consuming laboratory construction is not needed, and the sudden detection capability shortage of a specific area is compensated by the function of rapid cross-area allocation of the existing nucleic acid detection capability; meanwhile, the mobile sampling and detection capability can effectively avoid concentrated transportation and aggregation of a large number of high-risk groups, and the method is favorable for orderly development of large-scale nucleic acid detection matters.

Drawings

FIG. 1 is a schematic diagram of a first embodiment of the mobile pathogen nucleic acid detection laboratory system of the present invention supporting rapid deployment.

Fig. 2 is a schematic view of a second embodiment of the present invention.

Fig. 3 is a schematic view of a third embodiment of the present invention.

Fig. 4 is a schematic view of a fourth embodiment of the present invention.

Description of the figure numbering: 1-reagent storage and preparation movable structure, 11-reagent preparation module, 111-nucleic acid detection reagent preparation assembly, 112-nucleic acid detection reagent subpackaging assembly, 113-nucleic acid detection reagent storage assembly, 12-first buffer module, 2-specimen preparation movable structure, 21-nucleic acid processing module, 211-sample inactivation assembly, 212-sample nucleic acid extraction assembly, 213-nucleic acid sample adding assembly, 214-test reagent sealing assembly to be detected, 22-external sample collection device, 23-second buffer module, 3-amplification and product analysis movable structure, 31-nucleic acid amplification and data analysis module, 32-third buffer module, 323-power supply assembly, 4-airtight transfer window, 5-commercial power supply, 6-detachable cable, 323-power supply assembly, 6-detachable cable, power supply assembly, power supply, 7-sunshade, 8-waste disposal device.

Detailed Description

For a clearer understanding of the present invention, reference will be made to the following detailed description of the embodiments with reference to the accompanying drawings.

At present, pathogen nucleic acid detection laboratories are generally arranged in fixed facilities such as hospitals or related research institutes, and detection samples need to be collected and then are intensively sent to the detection laboratories for detection. Such a laboratory setting mode is inconvenient for short-term expansion of detection capability and reasonable configuration of detection capability, and often results in the situation that detection capability of key areas is seriously insufficient, and simultaneously detection capability of non-hotspot areas is greatly idle and wasted, which does not meet practical requirements of epidemic situation resistance.

Some movable laboratory designs are also proposed in the prior art, but most of the movable laboratory designs adopt an integrated structure designed for a large bus chassis, which is not beneficial to mutual isolation among various areas and has poor detection applicability to pathogens with strong infectivity.

FIG. 1 shows a schematic diagram of a first embodiment of the mobile pathogen nucleic acid detection laboratory system supporting rapid deployment according to the present invention, which comprises a reagent storage and preparation mobile structure 1, a specimen preparation mobile structure 2, and an amplification and product analysis mobile structure 3, which are isolated from each other and independent of each other. The reagent storage and preparation movable structure 1 corresponds to a reagent storage and preparation area in a nucleic acid detection laboratory and is responsible for preparing a stored reagent, subpackaging the reagent and preparing an amplification reaction mixed solution and storing and preparing consumables such as a centrifuge tube, a suction head and the like; the sample preparation movable structure 2 corresponds to a sample preparation area in a nucleic acid detection laboratory and is responsible for extracting, storing and adding nucleic acid (RNA and DNA) into an amplification reaction tube, particularly, the sample preparation movable structure 2 can relate to the operation of clinical samples and can meet the requirements of biological safety secondary laboratory protection equipment, personal protection and operation specifications; the movable amplification and product analysis structure 3 corresponds to an amplification area and an amplification product analysis area in a nucleic acid detection laboratory and is responsible for cDNA synthesis, DNA amplification and detection, and further analysis and determination of amplified fragments, such as hybridization, enzyme digestion electrophoresis, denaturing high performance liquid analysis, sequencing and the like. The reagent storage and preparation mobile structure 1 comprises a reagent dispensing module 11 maintaining a positive pressure environment, at least one airtight transfer window 4 arranged at a side wall of the reagent dispensing module 11, and a first buffer module 12 connecting an external environment and the reagent dispensing module 11 to form an airtight personnel access; the reagent preparation module 11 is provided with a nucleic acid detection reagent preparation component 111, a nucleic acid detection reagent subpackaging component 112 and a nucleic acid detection reagent storage component 113; the movable specimen preparation structure 2 comprises a nucleic acid processing module 21 maintaining a negative pressure environment, at least one airtight transmission window 4 arranged on the side wall of the nucleic acid processing module 21, a second buffer module 23 connecting the external environment and the nucleic acid processing module 21 to form an airtight personnel passage, and a deployable/retractable external specimen collecting device 22 preferably arranged on the outer side of the movable specimen preparation structure 2, wherein at least one airtight transmission window 4 is preferably arranged between the external specimen collecting device 22 and the nucleic acid processing module 21 to facilitate the transmission of the collected specimen; the nucleic acid processing module 21 is provided with a sample inactivation component 211, a sample nucleic acid extraction component 212, a nucleic acid sample adding component 213 and a to-be-detected reagent sealing component 214; the movable amplification and product analysis structure 3 comprises a nucleic acid amplification and data analysis module 31 maintaining a negative pressure environment, at least one airtight transmission window 4 disposed on a side wall of the nucleic acid amplification and data analysis module 31, and a third buffer module 32 connecting an external environment and the nucleic acid amplification and data analysis module 31 to form an airtight personnel passage, and the third buffer module 32 also houses a movable power supply component 323. In order to avoid secondary pollution caused by wastes generated in the nucleic acid detection process, the laboratory system preferably further comprises a waste treatment device 8, wherein the waste treatment device 8 can be separately and hermetically arranged in the movable specimen preparation structure 2 as shown in fig. 1, can be arranged in the movable amplification and analysis structure 3 or respectively arranged in the movable amplification and analysis structure or in a safe position outside the vehicle according to needs; the waste treatment apparatus 8 may include a harmless treatment device such as a high temperature and high pressure treatment device, an incineration device, and an airtight waste temporary storage device. The three movable structures can move respectively without interference and can be quickly unfolded at a required place to form a nucleic acid detection laboratory. A first embodiment of the reagent storage and preparation mobile structure 1, the specimen preparation mobile structure 2 and the amplification and product analysis mobile structure 3 is shown in fig. 1 deployed in an open field. In the first embodiment, the reagent storage and preparation movable structure 1, the specimen preparation movable structure 2 and the amplification and product analysis movable structure 3 are sequentially and equidistantly unfolded side by side, and a sunshade 7 is arranged on the right side, and the distance between the three movable structures is equal to the width of the sunshade 7 or larger than the width of the sunshade 7, so as to form a natural buffer zone; the right side of the reagent storage and preparation mobile structure 1 is provided with an airtight transfer window 4 facing the specimen preparation mobile structure 2; the left side and the right side of the movable specimen preparation structure 2 are respectively provided with an airtight transfer window 4, wherein the airtight transfer window 4 arranged on the left side faces the reagent storage and preparation structure 1 so as to be convenient for storing the reagent and preparing the movable specimen preparation structure 1 for transferring articles, the airtight transfer window 4 arranged on the right side faces the movable specimen amplification and product analysis structure 3, and meanwhile, an external specimen collecting device 22 is preferably arranged below a sunshade 7 on the right side of the movable specimen preparation structure 2 so as to be convenient for transferring materials. In embodiment 1, the reagent storage and preparation mobile structure 1, the specimen preparation mobile structure 2, and the amplification and product analysis mobile structure 3 are all connected to a commercial power supply 5 through a detachable cable 6.

As shown in fig. 2, the second embodiment of the present invention is similar to the first embodiment and also opens up a wide area, but different from the first embodiment, in order to deal with the situation of queuing and detecting a large number of people to be detected (for example, dense people areas such as schools, railway stations, etc.) in the second embodiment, the specimen preparation movable structure 2 is disposed at a position protruding from the reagent storage and preparation movable structure 1 and the amplification and product analysis movable structure 3, so that both sides of the specimen preparation movable structure 2 form enough open areas, and a sunshade 7 and an external sample collecting device 22 are disposed on both sides of the specimen preparation movable structure 2, so as to be able to handle more sample collecting works. The reagent storage and preparation mobile structure 1 and the amplification and product analysis mobile structure 3 are arranged at a position far away from the specimen preparation mobile structure 2, so that the pollution of a large number of people in line to a pathogen-free sanitary area can be further avoided.

Fig. 3 is a schematic view of a third embodiment of the present invention, which is used to deploy a laboratory corresponding to a narrow place, such as a residential area in a narrow road. In the third embodiment, the reagent storage and preparation movable structure 1, the specimen preparation movable structure 2 and the amplification and product analysis movable structure 3 are sequentially placed and unfolded from front to back, and sunshade canopies 7 can be arranged at the front and back positions of the movable structures, so that the reagent storage and preparation movable structures can be unfolded under the width of a standard road lane; the external sample acquiring device 22 and the airtight transfer windows 4 provided on each movable structure are preferably provided on the same side for easy movement and use by personnel. Should not be convenient for insert the condition of commercial power supply 5 simultaneously, store and prepare movable structure 1, sample preparation movable structure 2 and amplify and produce analysis movable structure 3 by the power supply subassembly 323 that sets up on the mobile structure 3 of amplification and produce analysis and supply power to the reagent, under the condition of using power supply subassembly 323 power supply, the single connection length of can dismantling cable 6 should be not more than 10 meters in order to guarantee power supply safety. The power supply component 323 is housed within the third buffer module 32 without being enabled; when the cable is started, the cable is moved to the external environment, the placement position of the power supply assembly 323 can be freely selected according to the arrangement positions of the three movable structures, and the detachable cable arrangement connection is facilitated. The power supply module 323 may preferably use a power supply device such as a diesel generator that can continuously output a stable voltage.

As shown in fig. 4, the present invention according to a fourth embodiment is similar to the third embodiment and is also developed for a corresponding narrow field laboratory, but different from the third embodiment, in the fourth embodiment, the power supply module 323 is directly connected and supplies power to the amplification and product analysis movable structure 3 only through the detachable cable 6, and the amplification and product analysis movable structure 3 is further connected in series with the specimen preparation movable structure 2 and the reagent storage and preparation movable structure through the detachable cable 6 to form a series power supply structure. In the fourth embodiment, since the series power supply structure connected in series is adopted, the limitation of the placement position of the power supply module 323 is further reduced, and it is possible to realize a longer distance power supply to the movable structure without considering the length limitation of the single detachable cable 6 connecting the power supply module 323.

Airtight pass-through box 4 can guarantee the structure gas tightness in the article transmission process through opening first airtight door and second airtight door in proper order including the first airtight door and the second airtight door that open simultaneously. Of course other airtight pass-through box designs can also be used in the present invention to achieve the same technical effect.

First buffer module 12, second buffer module 23 and third buffer module 32 all provide positive pressure environment's preventing leaking personnel's passageway, the structure that the connecting door between its and the external environment is preferred to be opened to buffer module internal rotation, can experience the appropriate resistance that is formed by positive pressure environment when making personnel open the connecting door from the external environment, also can utilize simultaneously between buffer module and the external environment pressure differential promotion connecting door closed by oneself. Further, the internal pressure of the first buffer module 12, the second buffer module 23 and the third buffer module 32 is higher than that of the functional module connected thereto, and particularly, the internal pressure of the first buffer module 12 is higher than the positive pressure value of the reagent preparing module 11 connected thereto. For example, if the pressure value of the reagent preparation module 11 is 15Pa and the pressure values of the nucleic acid processing module 21 and the nucleic acid amplification and data analysis module 31 are-15 Pa, the pressure value of the first buffer module 12 may be set to 30Pa and the pressure values of the second buffer module 23 and the third buffer module 32 may be set to 15 Pa. The first buffer module 12, the second buffer module 23 and the third buffer module 32 can provide necessary functions of changing clothes, sterilizing and the like for people, and can also be preferably provided with a function of detecting rest of people, such as a washroom, a bathroom and the like.

The above description is only for the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A mobile pathogen nucleic acid detection laboratory system supporting rapid deployment comprising reagent storage and preparation mobile structures, specimen preparation mobile structures and amplification and product analysis mobile structures isolated from each other;

2. The system of claim 1, wherein the specimen preparation mobile structure and/or the amplification and analysis mobile structure further comprises a separate hermetically isolated waste disposal device.

3. The system of claim 1, wherein the first, second, and third buffer modules each maintain a positive pressure environment, and wherein the positive pressure ambient pressure value of the first buffer module is higher than the reagent compounding module positive pressure ambient pressure value.

4. The system of claim 1, wherein the specimen preparation mobile structure further comprises an extendable/retractable external sample collection device; at least one airtight transfer window is arranged between the external sample collection device and the nucleic acid processing module.

5. The system of claim 1, wherein the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and the amplification and product analysis mobile structure are each separately mains powered by a detachable cable connection.

6. The system of claim 1, wherein the amplification and product analysis moveable structure further comprises a power supply component; the power supply assembly connects the reagent storage and preparation mobile structure, the specimen preparation mobile structure, and/or the amplification and product analysis mobile structure via a detachable cable.

7. The system of claim 6, wherein the power supply assembly is removably received within the third buffer module, and wherein a length of any detachable cable connected to the power supply assembly is 10 meters or less.

8. A system as claimed in any one of claims 1 to 7, wherein the movable structure is provided with at least one deployable/retractable awning in at least one of its directions; the unfolding width of the sunshade is not less than 3 m.

9. The system of claim 8, wherein the reagent storage and preparation mobile structures, the specimen preparation mobile structures, and the amplification and product analysis mobile structures are stationarily disposed at a linear distance from each other that is no less than the sunshade deployment width.

10. A system according to any one of claims 1 to 7, wherein the movable structure comprises a van, van trailer or container having a hermetically sealed cab.