WO2022041139A1

WO2022041139A1 - Integrated smart nucleic acid detection system

Info

Publication number: WO2022041139A1
Application number: PCT/CN2020/112233
Authority: WO
Inventors: 林思远; 李景; 樊清华; 邹良英; 彭友雄; 余德健; 蒋慧
Original assignee: 深圳华大智造科技股份有限公司
Priority date: 2020-08-28
Filing date: 2020-08-28
Publication date: 2022-03-03
Also published as: ZA202303702B; CN115885032A; CN115885032A8

Abstract

Provided is an integrated smart nucleic acid detection system, comprising: a sample processing unit used for extracting a nucleic acid sample from a sample to be detected; a sample detection unit used for detecting the nucleic acid sample to obtain a detection result; and an information management unit used for obtaining the detection result and outputting same. The integrated smart nucleic acid detection system provided by the present application can achieve quick and automatic nucleic acid detection.

Description

Integrated intelligent nucleic acid detection system

technical field

The present application relates to an integrated intelligent nucleic acid detection system.

Background technique

With the development of the new coronavirus (2019-nCoV) epidemic, there is a need for faster, more flexible and safer virus detection and identification speed, throughput and safety. However, most of the current detection methods are carried out in fixed laboratories, which have defects such as long detection time, low detection efficiency, and low degree of automation, which pose potential safety hazards to operators.

Application content

In view of this, it is necessary to provide an integrated intelligent nucleic acid detection system capable of efficient automatic detection.

This application provides an integrated intelligent nucleic acid detection system, including:

The sample processing unit is used to extract nucleic acid samples from the samples to be tested.

The sample detection unit is used to detect the nucleic acid sample to obtain the detection result.

and an information management unit for acquiring and outputting the detection result.

In the embodiment of the present application, the sample processing unit includes at least one sample processing device, and the sample processing device is used to extract the nucleic acid sample in the sample to be detected.

In the embodiment of the present application, the sample processing device is a nucleic acid extractor.

In the embodiment of the present application, the sample processing unit further includes at least one sample dispensing device, and the sample dispensing device is used for dispensing the samples to be detected.

In the embodiment of the present application, the sample detection unit includes at least one detection device, and the detection device is an RT-PCR detector.

In the embodiment of the present application, the integrated intelligent nucleic acid detection system includes at least one laboratory, and each laboratory includes a case and a sample processing chamber and a sample detection chamber disposed in the case. The sample processing The unit is arranged in the sample processing chamber, and the sample detection unit is arranged in the sample detection chamber.

In the embodiment of the present application, the laboratory further includes a sample transfer device and a sample transfer device, the sample processing chamber receives a sample to be tested through the sample transfer device, and the sample detection chamber communicates through the sample transfer device the sample processing chamber.

In the embodiment of the present application, the laboratory further includes a reagent preparation room, and a reagent preparation unit is arranged in the reagent preparation room, and the reagent preparation unit is used for providing detection reagents.

In the embodiment of the present application, the laboratory further includes a reagent transfer device, and the sample processing chamber communicates with the reagent preparation chamber through the reagent transfer device.

In the embodiment of the present application, the integrated intelligent nucleic acid detection system includes two groups of laboratories, and the two groups of laboratories are communicated through another sample transfer device.

Compared with the prior art, the integrated intelligent nucleic acid detection system provided by the present application can realize rapid and automatic nucleic acid detection, has high detection efficiency, flexible throughput, and meets the needs of safety prevention and control.

Description of drawings

FIG. 1 is a block diagram of an integrated intelligent nucleic acid detection system provided by an embodiment of the present application.

FIG. 2 is a flowchart of information management of an integrated intelligent nucleic acid detection system provided by an embodiment of the present application.

FIG. 3 is a schematic structural diagram of an integrated intelligent nucleic acid detection system provided by a second embodiment of the present application.

FIG. 4 is a schematic structural diagram of an integrated intelligent nucleic acid detection system provided by a third embodiment of the present application.

FIG. 5 is a schematic structural diagram of an integrated intelligent nucleic acid detection system provided by a fourth embodiment of the present application.

Description of main component symbols

Integrated intelligent nucleic

acid detection system

100, 200, 300, 400

Environmental Control Unit 11

Reagent preparation unit 12

Reagent preparation room 23

storage device 121

Clean bench 122

Sample Delivery Device 13

Reagent Delivery Device 14

Sample processing unit 15

Sample processing room 25

Sample processing device 151

Sample dispensing device 152

Sample transfer device 16

Sample testing unit 17

Sample testing room 27

Detection device 171

Information Management Unit 18

Air conditioning and mechanical and electrical room 22

Ventilation 111

Water supply and drainage device 112

Power supply 113

Central Management Station 114

Box 21

Airtight door 211

The first locker room 24

The first launderette 241

Buffer room 26

Second locker room 28

Autoclave 281

Second launderette 282

The following specific embodiments will further illustrate the present application in conjunction with the above drawings.

detailed description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative work, all belong to the scope of protection of this application.

It should be noted that when a component is referred to as being "fixed to" another component, it can be directly on the other component or there may also be a centered component. When a component is considered to be "connected" to another component, it may be directly connected to the other component or there may be a co-existence of an intervening component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a co-existing centered component. The terms "vertical," "horizontal," "left," "right," and similar expressions are used herein for illustrative purposes only.

The system implementation described below is only illustrative, and the division of the modules or circuits is only a logical function division, and other division methods may be used in actual implementation. Furthermore, it is clear that the word "comprising" does not exclude other units or steps and the singular does not exclude the plural. Multiple units or means recited in the system claims can also be implemented by one and the same unit or means by means of software or hardware. The terms first, second, etc. are used to denote names and do not denote any particular order.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used herein in the specification of the application are for the purpose of describing specific embodiments only, and are not intended to limit the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to FIG. 1, the integrated intelligent nucleic acid detection system 100 provided in the embodiment of the present application is used for the detection of nucleic acid samples, and is specifically applied to the detection of nucleic acid samples of the new coronavirus, and includes a laboratory, and the laboratory includes a reagent preparation unit 12, The sample transfer device 13 , the reagent transfer device 14 , the sample processing unit 15 , the sample transfer device 16 , the sample detection unit 17 , the information management unit 18 , and the environmental control unit 11 .

The reagent preparation unit 12 is used to store necessary detection reagents. The reagent preparation unit 12 specifically includes a storage device 121 and a clean table 122, the storage device 121 is used for storing relevant reagents for detection, and the clean table 122 is used for reagent preparation operations.

In this embodiment, the reagent preparation unit 12 further includes necessary equipment such as a pipette set, a small centrifuge, and a vortex mixer.

The sample processing unit 15 is used for extracting a sample to be detected to obtain a nucleic acid sample, and specifically includes a sample processing device 151 .

In this embodiment, the detection reagent is transferred from the reagent preparation unit 12 to the sample processing unit 15 through the reagent transfer device 14 . The sample to be tested is received by the sample transfer device 13 .

In this embodiment, the sample processing device 151 is used to extract nucleic acid samples from the samples to be detected. The sample processing device 151 is specifically a nucleic acid extraction device (eg, RT-PCR instrument), which is mainly used for RNA extraction. This application can use but is not limited to the high-throughput automated sample preparation system developed by Shenzhen Huada Zhizao Technology Co., Ltd. (model: MGISP-960, theoretical throughput 192 cases/8h ~ 576 cases/24h; or MGISP-100B , the theoretical flux of 192 cases/8h ~ 576 cases/24h). It can achieve high-throughput nucleic acid extraction, greatly improving the detection efficiency; it can automatically complete the extraction of nucleic acid samples to the configuration of RT-PCR reaction system, requiring only a small amount of conventional auxiliary equipment; it integrates various functions and covers an area of less ^than 0.5m ; Use filter tips to prevent cross-contamination, the pipetting range is as low as 2μL, and good pipetting accuracy can ensure better extraction efficiency. Compatible with RT-PCR system and third-party magnetic bead RNA extraction kit (MGIEasy nucleic acid extraction kit), it has good extraction stability and high nucleic acid recovery rate, which can ensure the extraction success rate of low-concentration samples.

In another embodiment, the sample processing unit 15 further includes at least one sample dispensing device 152. The sample dispensing device 152 is mainly used for automatic dispensing of the samples to be tested, and the split samples to be tested then enter the sample processing device 151 for processing. Nucleic acid extraction. Specifically, the automatic extraction of high-throughput samples can be performed by, but not limited to, the automatic cup processing system (MGISTP-7000, 192 cases/40min) developed by Shenzhen Huada Zhizao Technology Co., Ltd. The fully automatic cup processing system can be adapted to the above-mentioned high-throughput automated sample preparation system. The sample dispensing device 152 can realize operations such as automatic cap opening, automatic scanning, automatic dispensing and automatic capping, and input the whole rack of original tubes, thereby realizing automatic loading and unloading of samples and consumables, and automatic storage of finished products. Compared with manual operation, the efficiency of the sample dispensing device 152 can be increased by 7 times; the detection throughput can be greatly improved, and the input of manpower and space can be saved. Fully automatic processing can avoid contact between laboratory personnel and virus samples; at the same time, it is equipped with HEPA negative pressure filtration system and ultraviolet disinfection system to effectively prevent the risk of pollution leakage. The use of the fully automatic cup dividing processing system reduces manual intervention time, simplifies and standardizes tedious operations, liberates manpower, reduces misoperation and biological pollution, and reduces production costs.

The sample detection unit 17 receives the nucleic acid sample from the sample processing unit 15 through the sample transfer device 16 , and the sample transfer device 16 can transmit the nucleic acid sample processed by the sample processing device 151 in the sample processing unit 15 to the sample detection unit 17 .

In this embodiment, the sample detection unit 17 includes at least one detection device 171 .

In this embodiment, the detection device 171 is used to perform an amplification reaction on the extracted RNA sample to obtain a detection result. Specifically, in this embodiment, the detection device 171 is a fluorescence quantitative PCR instrument, and the fluorescent quantitative PCR instrument here is an RT-PCR instrument, wherein the conventional fluorescence quantitative PCR instrument can be combined with the above-mentioned high-throughput automated sample preparation system and automatic analysis system. Cup handling system compatible.

The information management unit 18 communicates with the reagent preparation unit 12 , the sample processing unit 15 , the sample detection unit 17 and the environmental control unit 11 through a wired or wireless network to realize information management and process management of each link.

Referring to FIG. 2 , in this embodiment, the information management unit 18 mainly includes two parts, the first part is sample information management, and the second part is detection process management.

Among them, the first part, the sample information management is mainly for the input management of sample information at each stage. Specifically, the reagent preparation unit 12, the sample processing unit 15 and the sample detection unit 17 are all provided with information input devices (such as barcode scanners). ), which is used to collect sample information at each stage and transmit the information to the information management unit 18, so as to realize the whole-process information management.

Specifically include:

In the sample collection stage, the information of the subject and the sample submitted for inspection is imported.

In the sample preparation stage, the package unpacking and sample inventory are recorded.

In the sample detection stage, the information of the sample tube submitted for inspection is checked and the external sample barcode is bound to the internal sample barcode.

In the sample collection stage, sample storage and sample inventory management.

In the sample testing stage, review the test results and issue a test report.

Specifically, a corresponding barcode is attached to each container containing the sample, and the barcode of the corresponding sample at the corresponding stage is output to the information management unit 18 through the barcode scanning device, which can reduce the time for data entry and information verification of a large number of samples, and reduce mixed samples. It is convenient for sample storage management and sample traceability. At the same time, it can also assist in reviewing report results and reduce the time for manual review of reports.

Among them, the second part, detection process management is mainly to realize information management for each detection process. Specifically, the information management unit 18 is respectively bound with the sample processing device 151, the sample dispensing device 152 and the detection device 171, which can realize the above Remote monitoring of equipment.

Specifically include:

During the sample processing stage, monitor sample layout and transfer of samples into deep well plates.

In the sample processing stage, the relationship between the deep-well plate of the sample dispensing device 152 and the nucleic acid extraction plate of the sample processing device 151 is bound, so that the sample to be monitored is automatically transferred from the sample dispensing device 152 to the sample processing device 151. Simultaneously monitor the operating status of the nucleic acid extraction device.

In the sample detection stage, the relationship between the nucleic acid extraction plate of the sample processing device 151 and the RT-PCR plate of the detection device 171 is bound, so that the nucleic acid sample on the nucleic acid extraction plate is transferred to the RT-PCR plate for amplification reaction, and at the same time Automatically capture detected values.

In the sample testing stage, the confirmation of RT-PCR test results is realized, and the confirmed RT-PCR test results enter the first sample information management step for test result review and test including output.

Among them, the use of informatized remote information and process management can collect and summarize production and equipment information in real time, realize information visualization and transparency, monitor production capacity, equipment operation status, equipment performance and saturation, and provide data basis for decision-making, thereby improving Manage efficiency and maximize productivity. The information management unit 18 can specifically use the laboratory information management unit (the Huada ZLIMS system) developed by Shenzhen Huada Zhizao Technology Co., Ltd. to carry out information management of the entire detection process. The Huada ZLIMS system is used as a dedicated information management system for life science instruments. The platform can record experimental process data, monitor the real-time status of instruments and samples, schedule off-machine data to storage, integrate information analysis platform, start automated analysis, and form an end-to-end complete solution from sample entry to final report.

The environmental control unit 11 is mainly used to control the ambient temperature, ambient pressure, ambient humidity, ambient atmosphere, laboratory water supply and drainage, and laboratory power supply of the entire laboratory.

In this embodiment, the environmental control unit 11 mainly includes a ventilation device 111 , a water supply and drainage device 112 , a power supply device 113 and a central management station 114 .

The ventilation device 111 is used to control environmental factors such as experimental temperature, humidity, pressure, and atmosphere, the water supply and drainage device 112 is used to supply and drain water to the entire laboratory, the power supply device 113 is used to supply power to the entire laboratory, and the central management station 114 is used to manage Network communication of a real laboratory.

Referring to FIG. 3 , an embodiment of the present application provides an integrated intelligent nucleic acid detection system 200 , which includes a box body 21 , an air conditioner and an electromechanical room 22 , a reagent preparation room 23 , and a first dressing room arranged in sequence in the box body 21 . 24 . The sample processing room 25 , the buffer room 26 , the sample detection room 27 and the second dressing room 28 . The reagent preparation unit 12 is installed in the reagent preparation chamber 23 , the sample processing unit 15 is installed in the sample processing chamber 25 , and the sample detection unit 17 is installed in the sample detection chamber 27 .

The box 21 is roughly a box with a cuboid structure (such as a vehicle compartment), which adopts excellent thermal insulation and rainproof design. The ground in the box 21 and the test bench are all sterilizable materials.

Two ends of the box body 21 are provided with airtight doors 211, the airtight doors 211 at one end lead into the first dressing room 24, and the airtight doors 211 at the other end lead into the second dressing room 28.

The first dressing room 24 is located between the reagent preparation room 23 and the sample processing room 25, and a first washing tank 241 is arranged therein for the operator to enter the laboratory for cleaning. Wherein, the first dressing room 24 is provided with two closed doors 211 , which can respectively enter the reagent preparation room 23 and the sample processing room 25 .

The second dressing room 28 is located on the side of the sample testing room 27 away from the sample processing room 25. The second dressing room 28 is communicated with the sample testing room 27 through the other two closed doors 211, and is used for the operator to perform corresponding cleaning when leaving the laboratory. Wash work. The second dressing room 28 is provided with a high-pressure disinfection device 281 and a second decontamination tank 282. The high-pressure disinfection device 281 is used for high-pressure disinfection of portable laboratory supplies, and the second decontamination tank 282 is used for personal decontamination by operators.

The above-mentioned reagent preparation unit 12 is arranged in the reagent preparation room 23, and the ultra-clean table 122 is arranged on one side of the reagent preparation room 23, and a storage device 121 is also arranged on the same side. 122 is used to perform reagent preparation operations. The side opposite to the storage device 121 is provided with a reagent transfer device 14, wherein the reagent transfer device 14 is used to transfer the reagent into the sample processing chamber 25, and the reagent transfer device 14 is provided with a transfer channel and windows arranged on both sides of the transfer channel The transfer channel is provided with an illumination lamp and an ultraviolet sterilizer, and the reagent transfer device 14 can transfer the reagent to the sample processing chamber 25 conveniently and quickly.

Please refer to FIG. 3 and FIG. 4 in conjunction. The above-mentioned sample processing unit 15 is disposed in the sample processing chamber 25, and the sample processing device 151 can use, but is not limited to, an automatic sample preparation system (MGISP-100B, MGISP-100B, Theoretical throughput of 192 cases/8h～576 cases/24h), high-throughput automated sample preparation system (model: MGISP-960, theoretical throughput of 192 cases/8h～576 cases/24h) or nucleic acid extractor (model: NE384 ), the use of the above automatic sample preparation device can automatically process the sample and significantly improve the overall detection effect.

The sample processing chamber 25 communicates with the outside world through the sample transfer device 13 and receives samples to be detected. The sample transfer device 13 is provided with a transfer channel and windows arranged on both sides of the transfer channel, wherein an illumination lamp and an ultraviolet sterilizer are arranged in the transfer channel, and the sample transfer device 13 can conveniently and quickly transfer the sample to the sample processing chamber 25 .

Please refer to FIG. 5 , in another embodiment, the sample processing unit 15 further includes a sample dispensing device 152 , wherein the sample dispensing device 152 is disposed on one side of the sample processing chamber 25 and is used for dispensing the samples to be detected. It can be used but not limited to the high-throughput automatic cup separation processing system (MGISTP-7000, 192 cases/40min) developed by Shenzhen Huada Zhizao Technology Co., Ltd. The system has a large throughput, can significantly improve the detection efficiency, and help The laboratory realizes high-throughput detection.

Please refer to FIG. 3 to FIG. 5 , the above-mentioned sample detection unit 17 is disposed in the sample detection chamber 27 , the sample detection chamber 27 communicates with the sample processing chamber 25 through the sample transfer device 16 , and the detection device 171 is used for PCR amplification of nucleic acid samples , wherein a plurality of detection devices 171 (such as RT-PCR instruments) are arranged in the sample detection chamber 27, which can improve the detection efficiency.

In this embodiment, the airtight door 211 is a special airtight door, equipped with an insulating glass observation window, a door lock, a handle, a door closer, a lift sealing strip, an electronic interlock, and the like.

Please refer to FIG. 1. In addition, the integrated intelligent nucleic acid detection system 200 further includes a ventilation device 111. In order to ensure the safety of experiments and personnel, the entire assembly line adopts a DC air-conditioning system with fresh air and full exhaust air, and the number of air changes is greater than 12 times/h, the cleanliness level is 100,000, the indoor temperature is 18℃-26℃, the humidity is less than 75%-75%, and the noise is less than 60dB. The indoor pressure is the overall negative pressure, one-way airflow; the air supply and exhaust form is upward and downward; the design of the air outlet is reasonable and does not affect the airflow of the biological safety cabinet. The laboratory air outlet is equipped with a P3 laboratory-level double in-situ high-efficiency air exhaust filter device, installed on the side wall, and an electric biological airtight valve is installed on the upper part to prevent indoor polluted air from being discharged outdoors.

Please refer to FIG. 1 , the integrated intelligent nucleic acid detection system 200 further includes a water supply and drainage device 112 , which can be connected to domestic tap water and hot water, and set up a clean tap water outlet tank. Wearing protective clothing is equipped with hand sanitizers. The first changing room 24 and the second changing room 28 are equipped with stainless steel handwashing sinks, equipped with automatic induction faucets, soap dispensers, spray hand sanitizers, and hand dryers. The sample processing room 25 and the sample testing room 27 are equipped with PP experimental water tanks, single-port cold and hot water test faucets, and double-port eye washers. When dealing with the new crown epidemic, the upper and lower waters of these two rooms are closed and not used. There are no floor drains in each room of the laboratory. Drainage is water for washing hands. There is no sewage treatment device in the laboratory for storage, disinfection and sterilization, and it is directly discharged into the nearby sewage well. Optional small wastewater treatment system.

Please refer to FIG. 1 , the integrated intelligent nucleic acid detection system 200 further includes a power supply device 113 , and a 380V-63A industrial connector (one set of male and female) is reserved on the side of the casing of the box body 21 for power distribution connection, and the owner is responsible for the 380V-40KW power supply Lead to the laboratory electromechanical warehouse. Lighting and access control security devices are equipped with UPS power supply, and the power supply time is not less than 15min. The designed illumination for each experimental area is: 100lx for the air conditioner and electromechanical room 22, 200lx for the first dressing room 24, second dressing room 28 and buffer room 26, 300lx for the reagent preparation room 23, sample processing room 25 and sample detection room 27 . In addition to normal lighting, all experimental working rooms are equipped with ultraviolet germicidal lamps, and the first dressing room 24 and the second dressing room 28 are provided with time-delay switches for separate control. All lamps are clean lamps and all light sources are LED light sources. Lighting switches are controlled individually for each room.

Please refer to FIG. 1 , the integrated intelligent nucleic acid detection system 200 further includes a central management station 114, and the air conditioner and electromechanical room 22 are provided with four external network cable interfaces, one for the telephone, two for the network, and one for the security reservation. There are 8 switches in the warehouse, and information points are reserved in the laboratory to ensure the smooth transmission of experimental data and information. The hospital is responsible for external weak wire access, and a 5G wireless communication card is optional. All airtight doors 211 are provided with access control devices, rooms are provided with surveillance cameras, and memory is provided in the weak current box. An independent automatic control system is set up in the air-conditioning and electromechanical room 22, which is used for system configuration, programming, fault diagnosis, etc., and at the same time realizes centralized operation and provides effective and accurate data for management personnel, so that the laboratory equipment can be formed effectively and reasonably order to achieve effective energy conservation. A touch operation screen is arranged on the wall of the first dressing room 24 , and a differential pressure sensor with display is arranged in the second dressing room 28 , the sample processing room 25 and the sample detection room 27 .

The integrated intelligent nucleic acid detection system 100 provided in this application can combine multiple groups of laboratories according to different usage requirements. Specifically, this application provides three design schemes, namely high-throughput scheme, basic scheme and Emergency plan.

Please refer to FIG. 3 , the integrated intelligent nucleic acid detection system 200 provided in this embodiment is a single-cabin basic solution: theoretical throughput: 700/8h～2100/24h, the sample processing chamber 25 is equipped with a sample processing device 151 that adopts high Throughput automated sample preparation system (model: MGISP-960, MGISP-100B)) and nucleic acid extraction instrument (model: NE384), the laboratory management system used is the ZLIMS system manufactured by Huada.

Referring to FIG. 4 , the integrated intelligent nucleic acid detection system 300 provided in this embodiment is a dual-chamber high-throughput solution: combining the two integrated intelligent nucleic acid detection systems 200 provided by the above basic solutions to achieve high-throughput detection, two The communication between the two sample processing chambers 25 is realized between the groups through the sample transfer device 13, and the interaction of the samples to be detected is performed.

Referring to FIG. 5 , the integrated intelligent nucleic acid detection system 400 provided in this embodiment is a single-chamber high-throughput solution: theoretical throughput: 192/8h～576/24h, and the sample processing chamber 25 is equipped with a sample dispensing device 152 ( Model: MGISTP-7000) and sample processing device 151 (model: MGISP-100B), the laboratory management system used is the ZLIMS system manufactured by Huada.

The integrated intelligent nucleic acid detection system 100 provided by this application can move quickly with the car, and can meet nucleic acid detection anytime and anywhere, with a daily detection capacity of 1,000 people. The demand for flexible flux, fast automatic and safe prevention and control in the process. The main application scenarios include: local customs and ports of entry, airports, railway stations, long-distance bus stations, schools, exhibition halls, shopping malls, commercial buildings, government and commercial foreign aid.

The integrated intelligent nucleic acid detection system 100 provided by this application can be applied to the detection of the new coronavirus, and conforms to the technical guidelines for the laboratory detection of pneumonia caused by the new coronavirus infection (third edition), the Administrative Measures for Clinical Gene Amplification Testing Laboratories of Medical Institutions 2010, GB11489-2008 General Requirements for Laboratory Biosafety, WS-233-2017 General Guidelines for Biosafety of Microbial and Biomedical Laboratories, GB50849-2014: Design Specifications for Infectious Disease Hospital Buildings, T-CECS 662-2020 "Medical Biosafety Secondary Experiments" Laboratory Building Technical Standards, GB-50346-2011 Biosafety Laboratory Building Technical Specifications, GB-27421-2015 Mobile Laboratory Biosafety Requirements and other industry standards.

In addition, for those of ordinary skill in the art, various other corresponding changes and deformations can be made according to the technical concept of the present application, and all these changes and deformations should belong to the protection scope of the claims of the present application.

Claims

An integrated intelligent nucleic acid detection system, characterized in that it includes:

A sample processing unit for extracting nucleic acid samples from the samples to be tested;

a sample detection unit for detecting the nucleic acid sample to obtain a detection result; and

The information management unit is used for acquiring and outputting the detection result.
The integrated intelligent nucleic acid detection system according to claim 1, wherein the sample processing unit comprises at least one sample processing device, and the sample processing device is used for extracting nucleic acid samples in the samples to be detected.
The integrated intelligent nucleic acid detection system according to claim 2, wherein the sample processing device is a nucleic acid extractor.
The integrated intelligent nucleic acid detection system according to claim 2, wherein the sample processing unit further comprises at least one sample dispensing device, and the sample dispensing device is used for dispensing the samples to be detected.
The integrated intelligent nucleic acid detection system according to claim 1, wherein the sample detection unit comprises at least one detection device, and the detection device is an RT-PCR detector.
The integrated intelligent nucleic acid detection system according to claim 1, wherein the integrated intelligent nucleic acid detection system comprises at least one laboratory, and each laboratory comprises a box and a A sample processing chamber and a sample detection chamber, the sample processing unit is arranged in the sample processing chamber, and the sample detection unit is arranged in the sample detection chamber.
The integrated intelligent nucleic acid detection system according to claim 6, wherein the laboratory further comprises a sample transfer device and a sample transfer device, and the sample processing chamber receives the sample to be detected through the sample transfer device, and the The sample detection chamber communicates with the sample processing chamber through the sample transfer device.
The integrated intelligent nucleic acid detection system according to claim 6, wherein the laboratory further comprises a reagent preparation room, and a reagent preparation unit is arranged in the reagent preparation room, and the reagent preparation unit is used to provide detection reagents .
The integrated intelligent nucleic acid detection system according to claim 8, wherein the laboratory further comprises a reagent transfer device, and the sample processing chamber communicates with the reagent preparation chamber through the reagent transfer device.
The integrated intelligent nucleic acid detection system according to claim 7, wherein the integrated intelligent nucleic acid detection system comprises two groups of laboratories, and the two groups of laboratories are communicated through another sample transfer device.