CN109082422A - A kind of full-automatic PCR nucleic acid extraction detection device - Google Patents
A kind of full-automatic PCR nucleic acid extraction detection device Download PDFInfo
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- CN109082422A CN109082422A CN201811237845.7A CN201811237845A CN109082422A CN 109082422 A CN109082422 A CN 109082422A CN 201811237845 A CN201811237845 A CN 201811237845A CN 109082422 A CN109082422 A CN 109082422A
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Abstract
The present invention proposes that a kind of full-automatic PCR nucleic acid extraction detection device, the full-automatic PCR nucleic acid extraction detection device include: casing assembly, carrier assemblies, sample-adding component, matched reagent box rest area, PCR fluorescence detection component, control computer installation.The present invention realizes sample from the quick full automatic working for handling, extracting detection, isolated multi-section separating tests system organic combination on traditional market is integrated, automatic quick separating sample and PCR detection can be operated by single step, applicable sample includes blood, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.
Description
Technical field
The present invention relates to technical field of biological more particularly to a kind of full-automatic PCR nucleic acid extraction detection devices.
Background technique
The detection of in vitro sample medicine, which has, to be simplified, automates, close to advantages such as patient scenes, in clinical treatment and correlation
It is played an important role in medical research field.The processing of sample at present is low with detection the degree of automation, is not able to satisfy clinically
The demand of quickly timely, high-throughput test samples.
Nucleic acid molecules detection technique has a powerful advantages in laboratory medicine and clinical research, nucleic acid extraction often use pillar method,
Paramagnetic particle method etc., it is generally existing complicated for operation, bothersome, required cost is high the problems such as.Currently, detection of nucleic acids it is most widely used be real-time
Fluorescent PCR technology, but reagent is not mostly instant, has complicated configuration work before use, be easy to cause the mistake of test
Difference even failure.Different personnel's operations also cause experimental error；Certain reagents save unstable at normal temperature.Nucleic acid extraction detection
Device cannot accomplish one-step method rapidly extracting nucleic acid；PCR detection reagent needs on-site manual to configure, and it is a large amount of to be unable to full automatic treatment
Test sample, multiple steps need handmarking, and the detecting instrument in each stage is independent, lack integrate pattern detection overall process be
Bulk cargo is set.
Summary of the invention
In view of above-mentioned disadvantages described above existing in the prior art, the invention proposes a kind of full-automatic PCR nucleic acid extractions to examine
Survey device.The present invention provides a kind of automation, whole process PCR nucleic acid extraction detection device, realizes sample from handling, extract inspection
The quick full automatic working surveyed, the isolated multi-section separating tests system organic combination on traditional market is integrated, can be passed through
Single step operates automatic quick separating sample and PCR detection, and applicable sample includes blood, serum, blood plasma, saliva, urine, tissue
Liquid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.
The present invention provides a kind of PCR nucleic acid extraction detection device characterized by comprising casing assembly, carrier assemblies,
It is loaded component, matched reagent box rest area, PCR fluorescence detection component, control computer installation；The casing assembly is for accommodating
The operative body of detection device, and protective effect is carried out to operative body；Component is loaded for realizing sample, PCR reaction examination
Agent, automatic sucking, delivery and the sample-adding for extracting product；Carrier assemblies are set for matched reagent box rest area to be arranged
It is equipped with temperature control module, mixes module, carrier module；Temperature control module keeps suitable reaction temperature；Module is mixed for being loaded
Automatic mixing is afterwards to promote to react fully；Carrier module is used to the PCR pipe after amplified reaction being carried to PCR fluorescence detection group
Part carries out fluorescence detection；The first area of matched reagent box rest area is sample extraction area, for placing sample container and splitting
Solve liquid kit；The second area of matched reagent box rest area is for placing PCR pipe；PCR fluorescence detection component is according to the control
The quantitative fluorescent PCR program of computer installation setting processed carries out fluorescence detection；The control computer installation is drawn, is transported
It carries, the control monitoring of sample-adding, and carries out the setting of quantitative fluorescent PCR program.
Preferably, the carrier assemblies specifically include: carrier, recessed cavity；The lower cavity of the carrier setting
Body is for accommodating matched reagent box rest area；The recessed cavity is divided into the first cavity and the second cavity, wherein the first chamber
The size and shape of body matches with the sample container of placement to the first area and the size shape of cracking liquid kit,
The size and shape of second cavity and the size shape of the PCR pipe of placement to the second area match.
Preferably, the temperature control module of the carrier assemblies include: the first heating coil, the second heating coil, third heating coil,
4th heating coil, the first temperature inductor, second temperature inductor；Wherein, the first heating coil and the second heating coil are around described
First cavity of recessed cavity, for being heated for the cracking liquid kit that is placed in the first area, to remain suitable for
The temperature of nucleic acid cleavage；First heating coil, the second heating coil are arranged up and down, so that the first cavity be made to be heated evenly up and down；First
Heating coil, the second heating coil are cricoid electric heating piece.Third heating coil, the 4th heating coil surround the second chamber of the cavity body
Body, for being heated for the PCR pipe that is placed in the second area, to maintain the temperature for being suitable for amplified reaction；Third
Heating coil, the 4th heating coil are arranged up and down, keep the second cavity upper and lower part temperature uniform；Third heating coil, the 4th heating coil
It also is electric heating piece；And third heating coil, the 4th heating coil respectively have an opening, the opening of the two is in alignment with each other, thus
Allow the PCR pipe via the opening across third heating coil, the 4th heating coil, to pass in and out second cavity.
Preferably, the carrier module includes: transmission driving assembly and transmission sliding seat；The PCR pipe is second
It is placed in region on the transmission sliding seat, and transmitting driving assembly includes torsion arm and driving motor, wherein
The first end of transmission sliding seat and torsion arm is fixed, and the second end of torsion arm then connects the motor shaft of driving motor；From
And the rotation of the motor shaft by driving motor, it is driven through torsion arm and the transmission sliding seat is driven to slide；It is slided by transmission
Dynamic seat carries the PCR pipe sliding, can through third heating coil, the 4th heating coil opening and enter second cavity
It is interior, cracking nucleic acid product is carried out at the second cavity and removes the sample-adding of RNA enzyme water；Described in can also being carried as transmission sliding seat
PCR pipe moves to from the PCR fluorescence detection component from second cavity, glimmering to carrying out by the sample after amplified reaction
Light detection.
Preferably, the mixing module includes the first vibrating base, the first torsion component, the second vibrating base, second
Reverse component；Wherein the first vibrating base, the first torsion component apply vibration for cracking liquid kit intracorporal to the first chamber
And torsion, so that the sample that the kit is added be promoted to mix well with lysate；Second vibrating base and the second torsion
Component then drives the intracorporal PCR pipe of the second chamber to realize vibration and torsion, to promote the PCR reagent of freezing dry powder form and split
It solves nucleic acid product and RNA enzyme water is gone to be uniformly mixed.
Preferably, the sample-adding component includes the mechanical arm and automatic sampling gun of tri- axial action of X-Y-Z；The machine
Tool arm is contacted for carrying the automatic sampling gun with draw target；The automatic sampling gun, which is used to realize under contact condition, to be inhaled
It takes, and is added dropwise under contactless state.
Preferably, the mechanical arm is divided into upper arm, lower arm and support rod, wherein passes through between upper arm and support rod
The connection of the tri- axis omnidirectional joint X-Y-Z, upper arm can adjust angle in tri- axis direction of X-Y-Z, so that automatic sampling gun alignment is wanted
Suction and the target being added dropwise；It is connected between the lower arm and upper arm by unidirectional arthrodia, so that lower arm can be relative to upper
Arm is slided up and down to adjust the head end height of automatic sampling gun.
Preferably, the automatic sampling gun includes gun platform and sliding pipette tips, and the sliding pipette tips are located at the lower section of gun platform,
And it is equipped with buffer part between gun platform and sliding pipette tips, realize effective buffer function.
Preferably, the sliding pipette tips are equipped with fixing head back to gun platform side, install pipette above fixing head；
The fixing head is connected to vacuum and drives liquid system；It includes liquid storage chamber and vacuum suction syringe that the vacuum, which drives liquid system,.
Preferably, the PCR fluorescence detection component includes the test chamber that shines, by with black light-absorbing outside the test chamber that shines
The micropore closed plate omnidirectional of institute of material production surrounds, and has translucency micropore in micropore closed plate, passes through the translucency micropore
Export the optical signal of the generation in the luminous test chamber；Optical measuring instrument is used to incude the light exported by the translucency micropore
Signal strength；The optical signal input mouth of translucency micropore and the optical measuring instrument is connected with each other by fiber optic conduction component.
As it can be seen that the present invention realizes sample from the quick full automatic working for handling, extracting detection, collocation cracking liquid kit
And PCR pipe, it can complete to detect the real-time fluorescence nucleic acid molecules of polymorphic type body fluid sample with single step, applicable sample includes blood
Liquid, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.This hair
Bright carrier can provide the temperature suitable for nucleic acid cleavage and amplified reaction, and realize automatically and mix and convey operation；Sample-adding
Component can flexibly realize that sample is drawn and added, and have buffer structure, avoid contacting firmly and bring damage, and facilitate suction
Liquid pipe updates；Digitlization quantitative judge can be supported in fluorescence detection, with that recognition result is accurate, recognition speed is fast is excellent
Gesture.
Figure of description
Fig. 1 is the PCR nucleic acid extraction detection device configuration diagram of the preferred embodiment of the present invention.
Fig. 2 is the PCR nucleic acid extraction detection device carrier partial schematic diagram of the preferred embodiment of the present invention.
Fig. 3 is the PCR nucleic acid extraction detection device carrier module and mixing module section signal of the preferred embodiment of the present invention
Figure.
Fig. 4 is the PCR nucleic acid extraction detection device automatic sampling gun structural schematic diagram of the preferred embodiment of the present invention；
Fig. 5 is the PCR nucleic acid extraction detection device fluorescence detection component structure diagram of the preferred embodiment of the present invention.
Specific embodiment
Below by embodiment, technical solution of the present invention is described in further detail.
Fig. 1 is the full-automatic PCR nucleic acid extraction detection device configuration diagram of the preferred embodiment of the present invention.Full-automatic PCR
Nucleic acid extraction detection device includes: casing assembly 1, carrier assemblies 2, sample-adding component 3, matched reagent box rest area 4, PCR fluorescence
Detection components 5, control computer installation.The casing assembly 1 is used to accommodate the operative body of detection device, and to operative body
Carry out protective effect.Component 3 is loaded for realizing sample, PCR reaction reagent, the automatic sucking for extracting product, delivery and is added
Sample.Carrier assemblies 2 are provided with temperature control module, mix module, delivery mould for matched reagent box rest area 4 to be arranged
Block；Temperature control module keeps suitable reaction temperature；Mix module be used for after sample-adding automatic mixing to promote to react fully；Delivery
Module, which is used to for the PCR pipe after amplified reaction to be carried to PCR fluorescence detection component 5, carries out fluorescence detection.Matched reagent box is put
Setting area 4 includes following region: first area is sample extraction area, for placing sample container and cracking liquid kit,
Nucleic acid cleavage liquid built in liquid kit is cracked, component 3 need to be only loaded and sample sample to be tested such as blood etc. in turn from sample container
It is added to cracking liquid kit, the cracking of nucleic acid can be completed, release sample of nucleic acid completely；Second area is to preserve
The PCR pipe of PCR reagent is lyophilized, the PCR reagent of freezing dry powder form can be directly used in quantitative fluorescent PCR measurement after sample-adding,
The PCR reagent includes a plurality of types of PCR reaction buffers, archaeal dna polymerase, a variety of atopic primers and probe, composition
PCR amplification system；The cracking nucleic acid product in first area is directly added into the PCR pipe of second area by being loaded component 3
And RNA enzyme water is removed, automatic mixing is carried out by the mixing module of the carrier assemblies 2, liquid can in the PCR pipe finally obtained
It is directly used in fluorescence detection.The quantitative fluorescent PCR program that PCR fluorescence detection component 5 is arranged according to the control computer installation
Carry out fluorescence detection.The control monitoring that the control computer installation is drawn, delivered, is loaded, and carry out fluorescence
The setting of quantitative PCR program.
The nucleic acid rapidly extracting detection kit with this coordinative composition of equipments includes two parts, is cracking liquid kit respectively
And PCR pipe, wherein cracking liquid kit is placed on the first area；PCR pipe is placed in the second area.Lysate examination
Nucleic acid cleavage liquid is held in agent box, sample to be tested is added in the cracking liquid kit by sample-adding component 3, in the lysate
Under effect, virus structure can be made to change, release viral nucleic acid, the lysate is without inhibition real-time fluorescence PCR
Reacted constituent, product can be directly used for subsequent PCR detection.The freezing that placement is directly used in quantitative fluorescent PCR measurement in PCR pipe is done
Powder PCR amplification system reagent, the freezing dry powder PCR amplification system reagent be with a variety of PCR reaction buffers, archaeal dna polymerase,
A variety of atopic primers, probe and freeze drying protectant are the gains that raw material is prepared by freeze drying process.Sample-adding
The PCR pipe is added in cracking nucleic acid product in first area by component 3, and removes RNA enzyme water to PCR pipe addition, passes through microscope carrier
Component 2 mixes the automatic mixing of module, can directly execute fluorescence detection to it by PCR fluorescence detection component 5.
As shown in Fig. 2-Fig. 3, the carrier assemblies 2 are specifically included: carrier 201, recessed cavity 202, the first heating coil
203, the second heating coil 204, third heating coil 205, the 4th heating coil 206, the first temperature inductor 207, second temperature induction
Device 208, transmission driving assembly 209, transmission sliding seat 210, the first vibrating base 211, first torsion component 212, second vibrate
Pedestal 213, second reverses component 214.
Wherein, as shown in Fig. 2, the recessed cavity 202 that the carrier 201 is arranged is put for accommodating the matched reagent box
Set area 4, matched reagent box rest area 4 is divided for first area and second area, therefore the recessed cavity 202 is also classified into
One cavity and the second cavity, wherein the size and shape of the first cavity with place to the first area sample container and split
The size shape of solution liquid kit matches, size and shape and the PCR pipe of placement to the second area of the second cavity
Size shape matches.The temperature control module includes: the first heating coil 203, the second heating coil 204, third heating coil 205,
Four heating coils 206, the first temperature inductor 207, second temperature inductor 208.Wherein, the first heating coil 203 and the second heating
Circle 204 surround the first cavity of the recessed cavity 202, for the cracking liquid kit progress to be placed in the first area
Heating, to remain suitable for the temperature of nucleic acid cleavage；First heating coil 203, the arrangement of the second about 204 heating coil, to make first
Cavity is heated evenly up and down；First heating coil 203, the second heating coil 204 are cricoid electric heating piece.Third heating coil 205,
Four heating coils 206 around the cavity body 202 the second cavity, for being added for the PCR pipe that is placed in the second area
Heat, to maintain the temperature for being suitable for amplified reaction；Third heating coil 205, the 4th heating coil 206 are also arrangement up and down, make the
Two cavity upper and lower part temperature are uniform；Also, third heating coil 205, the 4th heating coil 206 are also electric heating piece；And third
Heating coil 205, the 4th heating coil 206 are not closed annular, but respectively have an opening, and the opening of the two is right each other
Together, to allow the PCR pipe via the opening across third heating coil 205, the 4th heating coil 206, thus described in disengaging
Second cavity, to realize delivery of the PCR pipe between carrier assemblies 2 and PCR fluorescence detection component 5.The first temperature sense
Device 207 is answered to adjust first heating for monitoring the intracorporal real time temperature of the first chamber, and according to the temperature value of monitoring
The calorific value of circle 203 and the second heating coil 204, to remain suitable for the temperature of nucleic acid cleavage.Second temperature inductor 208 is used for
The real time temperature of second cavity is monitored, and third heating coil 205 and the 4th heating coil are adjusted according to the temperature value of monitoring
206 calorific value, to maintain the temperature for being suitble to amplified reaction.
As shown in figure 3, the carrier module includes transmission driving assembly 209 and transmission sliding seat 210.The PCR pipe
Be placed on the transmission sliding seat 210 in the second area, and transmit driving assembly 209 include torsion arm 209A with
And driving motor 209B, wherein transmission sliding seat 210 and the first end of torsion arm 209A are fixed, and the second of torsion arm 209A
End then connects the motor shaft of driving motor 209B.To by the rotation of the motor shaft of driving motor 209B, through torsion arm
209A is driven and the transmission sliding seat 210 is driven to slide.PCR pipe sliding is carried by transmitting sliding seat 210, it can be with
Through third heating coil 205, the 4th heating coil 206 opening and enter in second cavity, split at the second cavity
Solution nucleic acid product and the sample-adding for removing RNA enzyme water；The PCR pipe can also be carried by transmission sliding seat 210 from second chamber
Body moves at the PCR fluorescence detection component 5, carries out fluorescence detection to by the sample after amplified reaction.
The mixing module includes that the first vibrating base 211, first reverses component 212, the second vibrating base 213, second
Reverse component 214.Wherein the first vibrating base 211, first torsion component 212 is used for the intracorporal cracking liquid kit of the first chamber
Apply vibration and torsion, so that the sample that the kit is added be promoted to mix well with lysate；First vibrating base 211 by
Eccentric vibrating motor provides power to realize vibration, and the first torsion component 212 is driven by torsion motor in certain angle range
It is interior back and forth to be reversed.Second vibrating base 213 and the second torsion component 214 then drive the intracorporal PCR pipe of the second chamber to realize
Vibration and torsion, so that the PCR reagent of freezing dry powder form be promoted nucleic acid product and RNA enzyme water to be gone to mix with cracking
It is even.
Sample to be tested such as blood etc. is sampled from sample container and then is added to cracking liquid kit by sample-adding component 3, and
Cracking nucleic acid product is added into PCR pipe and removes RNA enzyme water.The sample-adding component 3 includes the machinery of tri- axial action of X-Y-Z
Arm 301 and automatic sampling gun 302.Such as Fig. 1, mechanical arm 301 divides for upper arm 301A, lower arm 301B and support rod 301C,
In, it is connected between upper arm 301A and support rod 301C by the tri- axis omnidirectional joint X-Y-Z, therefore upper arm 301A can be in X-Y-Z
Angle is adjusted in three axis directions, so that (target includes that sample holds to the target that the alignment of automatic sampling gun 302 will be aspirated and is added dropwise
Device, cracking liquid kit and PCR pipe).It is connected between lower arm 301B and upper arm 301A by unidirectional arthrodia, thus lower arm
301B can be slided up and down relative to upper arm 301A to adjust the head end height of automatic sampling gun 302, realize the automatic sampling gun
Contact between 302 and draw target is realized under contact condition and is drawn, and then moves up realization separation.
As shown in figure 4, automatic sampling gun 302 is equipped with gun platform 302A and sliding pipette tips 302B, sliding pipette tips 302B
In the lower section of gun platform 302A, and between be equipped with buffer part 302C.Buffer part 302C is mounted on gun platform 302A and sliding pipette tips 302B
Between, realize effective buffer function, when being loaded, when making 302 contact target of automatic sampling gun by mechanical arm 301,
Automatic sampling gun 302 continues traveling downwardly, and buffer part 302C plays a role at this time, enables between automatic sampling gun 302 and target
Effectively fitting.The buffer spring 302H that the buffer part 302C specifically includes guide post 302G and is set on guide post 302G, it is described
The one end guide post 302G is fixedly connected with sliding pipette tips 302B, and the other end passes through gun platform 302A and is slidably matched with it, the buffering elastic
Spring 302H is set between gun platform 302A and sliding pipette tips 302B.The sliding pipette tips 302B is equipped with back to the side gun platform 302A and fixes
Pipette 302E is installed, the fixing head 302D is connected to vacuum and drives liquid system 302F above head 302D, fixing head 302D.It is described
It includes liquid storage chamber 302I and vacuum suction syringe 302J that vacuum, which drives liquid system 302F,；When executing absorption operation, vacuum suction
Syringe 302J provides vacuum force, and liquid is pumped to liquid storage chamber 302I through fixing head 302D and is saved；Make when being added dropwise
When industry, vacuum suction syringe 302J provide thrust, and liquid is released from liquid storage chamber 302I through fixing head 302D.Pipette 302E
Through sliding sleeve 302K in conjunction with fixing head 302D, therefore pipette 302E can be realized from fixing head by moving up sliding sleeve 302K
Automatic drawing unloading on 302D.
Nucleic acid product will cracked by sample-adding component 3 and going RNA enzyme water that PCR pipe is added, and by mixing well completion
After amplified reaction, which is sent to the PCR fluorescence detection component 5 by the carrier module, by the PCR fluorescence
Detection components 5 carry out fluorescence detection according to the quantitative fluorescent PCR program of the control computer installation setting.The PCR fluorescence
Detection components 5 include the test chamber 501 that shines, the micropore closed plate made outside the test chamber 501 that shines of black light-absorbing material
502 omnidirectionals surround, these avoid the interference effect of extraneous light.There is translucency micropore 503 in micropore closed plate 502,
The optical signal of the generation in the luminous test chamber 501 is exported by the translucency micropore.Optical measuring instrument 504 is logical for incuding
Cross the light signal strength that the translucency micropore 503 exports.The optical signal of translucency micropore 503 and the optical measuring instrument 504 is defeated
Inbound port by fiber optic conduction component 505 be connected with each other, the optical measuring instrument further include optical gain converter, signal amplifier with
And analog to digital conversion circuit, optical gain converter and signal amplifier are used to convert optical signal into electric signal and be amplified,
And then will be electric signal digitising by analog to digital conversion circuit, final output quantifies table generated by signal acquisition, amplification, sampling
Show the digital signal of the light signal strength.In one-shot measurement, acquisition light signal strength is acquired repeatedly.Control computer installation
For receiving the digital signal of the transmission of optical measuring instrument 504, based on a large amount of light signal strength, generation and sample to be tested
Corresponding luminosity curve, luminosity curve indicate the relationship of light signal strength and time.And it controls computer installation also to prestore
Several standard curves, the corresponding reference value of every standard curve are stored up.Luminosity curve and standard curve are fitted, produced
Raw measurement numerical value.
As it can be seen that the present invention realizes sample from the quick full automatic working for handling, extracting detection, collocation cracking liquid kit
And PCR pipe, it can complete to detect the real-time fluorescence nucleic acid molecules of polymorphic type body fluid sample with single step, applicable sample includes blood
Liquid, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.This hair
Bright carrier can provide the temperature suitable for nucleic acid cleavage and amplified reaction, and realize automatically and mix and convey operation；Sample-adding
Component can flexibly realize that sample is drawn and added, and have buffer structure, avoid contacting firmly and bring damage, and facilitate suction
Liquid pipe updates；Digitlization quantitative judge can be supported in fluorescence detection, with that recognition result is accurate, recognition speed is fast is excellent
Gesture.
Above embodiments are merely to illustrate the present invention, and not limitation of the present invention, the common skill in relation to technical field
Art personnel can also make a variety of changes and modification without departing from the spirit and scope of the present invention, therefore all etc.
Same technical solution also belongs to scope of the invention, and scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a kind of full-automatic PCR nucleic acid extraction detection device characterized by comprising casing assembly, carrier assemblies, sample-adding group
Part, matched reagent box rest area, PCR fluorescence detection component, control computer installation；The casing assembly is for accommodating detection dress
The operative body set, and protective effect is carried out to operative body；Component is loaded for realizing sample, PCR reaction reagent, extraction
Automatic sucking, delivery and the sample-adding of product；Carrier assemblies are provided with temperature control for matched reagent box rest area to be arranged
Module mixes module, carrier module；Temperature control module keeps suitable reaction temperature；Module is mixed for mixing automatically after sample-adding
It is even to be reacted fully with rush；Carrier module is used to for the PCR pipe after amplified reaction to be carried to the progress of PCR fluorescence detection component glimmering
Light detection；The first area of matched reagent box rest area is sample extraction area, for placing sample container and lysate reagent
Box；The second area of matched reagent box rest area is for placing PCR pipe；PCR fluorescence detection component is according to the control computer
The quantitative fluorescent PCR program of device setting carries out fluorescence detection；What the control computer installation was drawn, and was delivered, is loaded
Monitoring is controlled, and carries out the setting of quantitative fluorescent PCR program.
2. full-automatic PCR nucleic acid extraction detection device according to claim 1, which is characterized in that the carrier assemblies tool
Body includes: carrier, recessed cavity；The recessed cavity of the carrier setting is for accommodating matched reagent box rest area；
The recessed cavity is divided into the first cavity and the second cavity, wherein the size and shape of the first cavity and placement to firstth area
The sample container in domain and the size shape for cracking liquid kit match, and the size and shape of the second cavity and placement are to described
The size shape of the PCR pipe of second area matches.
3. full-automatic PCR nucleic acid extraction detection device according to claim 2, which is characterized in that the carrier assemblies
Temperature control module includes: the first heating coil, the second heating coil, third heating coil, the 4th heating coil, the first temperature inductor, second
Temperature inductor；Wherein, the first heating coil and the second heating coil around the recessed cavity the first cavity, for be placed in
The cracking liquid kit of the first area is heated, to remain suitable for the temperature of nucleic acid cleavage；First heating coil, second add
Gas ket is arranged up and down, so that the first cavity be made to be heated evenly up and down；First heating coil, the second heating coil are cricoid electric heating
Piece；Third heating coil, the 4th heating coil around the cavity body the second cavity, for be placed in the second area
PCR pipe is heated, to maintain the temperature for being suitable for amplified reaction；Third heating coil, the 4th heating coil are arranged up and down, make the
Two cavity upper and lower part temperature are uniform；Third heating coil, the 4th heating coil are also electric heating piece；And third heating coil, the 4th
Heating coil respectively has an opening, and the opening of the two is in alignment with each other, to allow the PCR pipe via the opening across the
Three heating coils, the 4th heating coil, to pass in and out second cavity.
4. full-automatic PCR nucleic acid extraction detection device according to claim 3, which is characterized in that the carrier module packet
It includes: transmission driving assembly and transmission sliding seat；The PCR pipe be placed in the second area the transmission sliding seat it
On, and transmitting driving assembly includes torsion arm and driving motor, wherein the first end of transmission sliding seat and torsion arm is solid
It is fixed, and the second end of torsion arm then connects the motor shaft of driving motor；Thus by the rotation of the motor shaft of driving motor,
It is driven through torsion arm and the transmission sliding seat is driven to slide；PCR pipe sliding is carried by transmission sliding seat, it can be through the
Three heating coils, the 4th heating coil opening and enter in second cavity, cracking nucleic acid product is carried out at the second cavity
And remove the sample-adding of RNA enzyme water；It can also be moved to by the transmission sliding seat carrying PCR pipe from second cavity described
At PCR fluorescence detection component, fluorescence detection is carried out to by the sample after amplified reaction.
5. full-automatic PCR nucleic acid extraction detection device according to claim 4, which is characterized in that the mixing module packet
Include the first vibrating base, the first torsion component, the second vibrating base, the second torsion component；Wherein the first vibrating base, first are turned round
Turn component and apply vibration and torsion for cracking liquid kit intracorporal to the first chamber, to promote the sample that the kit is added
This is mixed well with lysate；Second vibrating base and the second torsion component then drive the intracorporal PCR pipe of the second chamber to realize vibration
Dynamic and torsion, thus promote the PCR reagent of freezing dry powder form with crack nucleic acid product and RNA enzyme water gone to be uniformly mixed.
6. full-automatic PCR nucleic acid extraction detection device according to claim 5, which is characterized in that the sample-adding component packet
Include the mechanical arm and automatic sampling gun of tri- axial action of X-Y-Z；The mechanical arm is for carrying the automatic sampling gun and taking out
Inhale target contact；The automatic sampling gun is used to realize under contact condition and draw, and is added dropwise under contactless state.
7. full-automatic PCR nucleic acid extraction detection device according to claim 6, which is characterized in that the mechanical arm is divided into
Upper arm, lower arm and support rod, wherein connected between upper arm and support rod by the tri- axis omnidirectional joint X-Y-Z, upper arm can be
Angle is adjusted in tri- axis direction of X-Y-Z, so that automatic sampling gun is directed at the target that aspirate and be added dropwise；The lower arm and upper arm it
Between connected by unidirectional arthrodia, thus lower arm can be slided up and down relative to upper arm with adjust the head end of automatic sampling gun height
Degree.
8. full-automatic PCR nucleic acid extraction detection device according to claim 7, which is characterized in that the automatic sampling gun
Including gun platform and sliding pipette tips, the sliding pipette tips are located at the lower section of gun platform, and are equipped with buffer part between gun platform and sliding pipette tips,
Realize effective buffer function.
9. full-automatic PCR nucleic acid extraction detection device according to claim 8, which is characterized in that the sliding rifle
Head is equipped with fixing head back to gun platform side, installs pipette above fixing head；The fixing head is connected to vacuum and drives liquid system；Institute
Stating vacuum and driving liquid system includes liquid storage chamber and vacuum suction syringe.
10. full-automatic PCR nucleic acid extraction detection device according to claim 9, which is characterized in that the PCR fluorescence inspection
Surveying component includes the test chamber that shines, micropore closed plate omnidirectional of the institute packet made outside the test chamber that shines of black light-absorbing material
It encloses, there is translucency micropore in micropore closed plate, the generation in the luminous test chamber is exported by the translucency micropore
Optical signal；Optical measuring instrument is used to incude the light signal strength exported by the translucency micropore；Translucency micropore and the light
The optical signal input mouth of measuring instrument is connected with each other by fiber optic conduction component.
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