CN109337793A - A kind of full-automatic nucleic acid extraction detection system - Google Patents
A kind of full-automatic nucleic acid extraction detection system Download PDFInfo
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- CN109337793A CN109337793A CN201811237779.3A CN201811237779A CN109337793A CN 109337793 A CN109337793 A CN 109337793A CN 201811237779 A CN201811237779 A CN 201811237779A CN 109337793 A CN109337793 A CN 109337793A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
The present invention proposes a kind of full-automatic nucleic acid extraction detection system.The system includes full-automatic PCR nucleic acid extraction detection device and matched nucleic acid rapidly extracting detection kit.The full-automatic PCR nucleic acid extraction detection device includes: casing assembly, carrier assemblies, sample-adding component, matched reagent box rest area, PCR fluorescence detection component, control computer installation；The nucleic acid rapidly extracting detection kit includes cracking liquid kit and PCR pipe.The present invention realizes sample from the quick full automatic working for handling, extracting detection, isolated multi-section separating tests system organic combination on traditional market is integrated, automatic quick separating sample and PCR detection can be operated by single step, applicable sample includes blood, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.
Description
Technical field
The present invention relates to technical field of biological more particularly to a kind of full-automatic nucleic acid extraction detection systems.
Background technique
The detection of in vitro sample medicine, which has, to be simplified, automates, close to advantages such as patient scenes, in clinical treatment and correlation
It is played an important role in medical research field.The processing of sample at present is low with detection the degree of automation, is not able to satisfy clinically
The demand of quickly timely, high-throughput test samples.
Nucleic acid molecules detection technique has a powerful advantages in laboratory medicine and clinical research, nucleic acid extraction often use pillar method,
Paramagnetic particle method etc., it is generally existing complicated for operation, bothersome, required cost is high the problems such as.Currently, detection of nucleic acids it is most widely used be real-time
Fluorescent PCR technology, but reagent is not mostly instant, has complicated configuration work before use, be easy to cause the mistake of test
Difference even failure.Different personnel's operations also cause experimental error；Certain reagents save unstable at normal temperature.Nucleic acid extraction detection
Device cannot accomplish one-step method rapidly extracting nucleic acid；PCR detection reagent needs on-site manual to configure, and it is a large amount of to be unable to full automatic treatment
Test sample, multiple steps need handmarking, and the detecting instrument in each stage is independent, lack integrate pattern detection overall process be
Bulk cargo is set.
Summary of the invention
In view of above-mentioned disadvantages described above existing in the prior art, the invention proposes a kind of full-automatic nucleic acid extraction detection systems
System.The system includes full-automatic PCR nucleic acid extraction detection device and matched nucleic acid rapidly extracting detection kit.The present invention
Sample is realized from the quick full automatic working for handling, extracting detection, by the isolated multi-section separating tests system on traditional market
Organic combination is integrated, can be operated by single step automatic quick separating sample and PCR detection, applicable sample include blood,
Serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.
The present invention provides a kind of full-automatic nucleic acid extraction detection system characterized by comprising full-automatic PCR nucleic acid mentions
Take detection device and nucleic acid rapidly extracting detection kit；Wherein
The full-automatic PCR nucleic acid extraction detection device includes: that casing assembly, carrier assemblies, sample-adding component, matched reagent box are put
Set area, PCR fluorescence detection component, control computer installation；The casing assembly is used to accommodate the operative body of detection device, and
Protective effect is carried out to operative body；Be loaded component for realizing sample, PCR reaction reagent, extract product automatic sucking,
Delivery and sample-adding；Carrier assemblies for matched reagent box rest area to be arranged, and be provided with temperature control module, mix module,
Carrier module；Temperature control module keeps suitable reaction temperature；Mixing module is used for the automatic mixing after sample-adding and is filled with promoting to react
Point；Carrier module, which is used to for the PCR pipe after amplified reaction to be carried to PCR fluorescence detection component, carries out fluorescence detection；Mating examination
The first area of agent box rest area is sample extraction area, for placing sample container and cracking liquid kit；Matched reagent box
The second area of rest area is for placing PCR pipe；The fluorescence that PCR fluorescence detection component is arranged according to the control computer installation
Quantitative PCR program carries out fluorescence detection；The control monitoring that the control computer installation is drawn, delivered, is loaded, with
And carry out the setting of quantitative fluorescent PCR program；
The nucleic acid rapidly extracting detection kit includes cracking liquid kit and PCR pipe, wherein cracking liquid kit is placed
In the first area；PCR pipe is placed in the second area；Nucleic acid cleavage liquid is held in cracking liquid kit, by sample-adding group
Sample to be tested is added in the cracking liquid kit by part, releases viral nucleic acid under lysate effect；It is described
The freezing dry powder PCR amplification system reagent for being directly used in quantitative fluorescent PCR measurement is placed in PCR pipe, the freezing dry powder PCR expands
Increasing system reagent is using PCR reaction buffer, archaeal dna polymerase, atopic primer, probe and freeze drying protectant as raw material
The gains prepared by freeze drying process.
Preferably, the carrier assemblies specifically include: carrier, recessed cavity；The lower cavity of the carrier setting
Body is for accommodating matched reagent box rest area；The recessed cavity is divided into the first cavity and the second cavity, wherein the first chamber
The size and shape of body matches with the sample container of placement to the first area and the size shape of cracking liquid kit,
The size and shape of second cavity and the size shape of the PCR pipe of placement to the second area match.
Preferably, the temperature control module of the carrier assemblies include: the first heating coil, the second heating coil, third heating coil,
4th heating coil, the first temperature inductor, second temperature inductor；Wherein, the first heating coil and the second heating coil are around described
First cavity of recessed cavity, for being heated for the cracking liquid kit that is placed in the first area, to remain suitable for
The temperature of nucleic acid cleavage；First heating coil, the second heating coil are arranged up and down, so that the first cavity be made to be heated evenly up and down；First
Heating coil, the second heating coil are cricoid electric heating piece.Third heating coil, the 4th heating coil surround the second chamber of the cavity body
Body, for being heated for the PCR pipe that is placed in the second area, to maintain the temperature for being suitable for amplified reaction；Third
Heating coil, the 4th heating coil are arranged up and down, keep the second cavity upper and lower part temperature uniform；Third heating coil, the 4th heating coil
It also is electric heating piece；And third heating coil, the 4th heating coil respectively have an opening, the opening of the two is in alignment with each other, thus
Allow the PCR pipe via the opening across third heating coil, the 4th heating coil, to pass in and out second cavity.
Preferably, the carrier module includes: transmission driving assembly and transmission sliding seat；The PCR pipe is second
It is placed in region on the transmission sliding seat, and transmitting driving assembly includes torsion arm and driving motor, wherein
The first end of transmission sliding seat and torsion arm is fixed, and the second end of torsion arm then connects the motor shaft of driving motor；From
And the rotation of the motor shaft by driving motor, it is driven through torsion arm and the transmission sliding seat is driven to slide；It is slided by transmission
Dynamic seat carries the PCR pipe sliding, can through third heating coil, the 4th heating coil opening and enter second cavity
It is interior, cracking nucleic acid product is carried out at the second cavity and removes the sample-adding of RNA enzyme water；Described in can also being carried as transmission sliding seat
PCR pipe moves to from the PCR fluorescence detection component from second cavity, glimmering to carrying out by the sample after amplified reaction
Light detection.
Preferably, the mixing module includes the first vibrating base, the first torsion component, the second vibrating base, second
Reverse component；Wherein the first vibrating base, the first torsion component apply vibration for cracking liquid kit intracorporal to the first chamber
And torsion, so that the sample that the kit is added be promoted to mix well with lysate；Second vibrating base and the second torsion
Component then drives the intracorporal PCR pipe of the second chamber to realize vibration and torsion, to promote the PCR reagent of freezing dry powder form and split
It solves nucleic acid product and RNA enzyme water is gone to be uniformly mixed.
Preferably, the sample-adding component includes the mechanical arm and automatic sampling gun of tri- axial action of X-Y-Z；The machine
Tool arm is contacted for carrying the automatic sampling gun with draw target；The automatic sampling gun, which is used to realize under contact condition, to be inhaled
It takes, and is added dropwise under contactless state.
Preferably, the mechanical arm is divided into upper arm, lower arm and support rod, wherein passes through between upper arm and support rod
The connection of the tri- axis omnidirectional joint X-Y-Z, upper arm can adjust angle in tri- axis direction of X-Y-Z, so that automatic sampling gun alignment is wanted
Suction and the target being added dropwise；It is connected between the lower arm and upper arm by unidirectional arthrodia, so that lower arm can be relative to upper
Arm is slided up and down to adjust the head end height of automatic sampling gun.
Preferably, the automatic sampling gun includes gun platform and sliding pipette tips, and the sliding pipette tips are located at the lower section of gun platform,
And it is equipped with buffer part between gun platform and sliding pipette tips, realize effective buffer function.
Preferably, the sliding pipette tips are equipped with fixing head back to gun platform side, install pipette above fixing head；
The fixing head is connected to vacuum and drives liquid system；It includes liquid storage chamber and vacuum suction syringe that the vacuum, which drives liquid system,.
Preferably, the PCR fluorescence detection component includes the test chamber that shines, by with black light-absorbing outside the test chamber that shines
The micropore closed plate omnidirectional of institute of material production surrounds, and has translucency micropore in micropore closed plate, passes through the translucency micropore
Export the optical signal of the generation in the luminous test chamber；Optical measuring instrument is used to incude the light exported by the translucency micropore
Signal strength；The optical signal input mouth of translucency micropore and the optical measuring instrument is connected with each other by fiber optic conduction component.
As it can be seen that the present invention realizes sample from the quick full automatic working for handling, extracting detection, collocation cracking liquid kit
And PCR pipe, it can complete to detect the real-time fluorescence nucleic acid molecules of polymorphic type body fluid sample with single step, applicable sample includes blood
Liquid, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.This hair
Bright carrier can provide the temperature suitable for nucleic acid cleavage and amplified reaction, and realize automatically and mix and convey operation；Sample-adding
Component can flexibly realize that sample is drawn and added, and have buffer structure, avoid contacting firmly and bring damage, and facilitate suction
Liquid pipe updates；Digitlization quantitative judge can be supported in fluorescence detection, with that recognition result is accurate, recognition speed is fast is excellent
Gesture.
Figure of description
Fig. 1 is the full-automatic nucleic acid extraction detection system configuration diagram of the preferred embodiment of the present invention.
Fig. 2 is the full-automatic nucleic acid extraction detection system carrier partial schematic diagram of the preferred embodiment of the present invention.
Fig. 3 is that the full-automatic nucleic acid extraction detection system carrier module of the preferred embodiment of the present invention and mixing module section show
It is intended to.
Fig. 4 is the full-automatic nucleic acid extraction detection system automatic sampling gun structural schematic diagram of the preferred embodiment of the present invention；
Fig. 5 is the full-automatic nucleic acid extraction detection system fluorescence detection component structure diagram of the preferred embodiment of the present invention.
Specific embodiment
Below by embodiment, technical solution of the present invention is described in further detail.
Fig. 1 is the full-automatic nucleic acid extraction detection system configuration diagram of the preferred embodiment of the present invention.The system includes: complete
Automatic PCR nucleic acid extraction detection device and matched nucleic acid rapidly extracting detection kit.
Full-automatic PCR nucleic acid extraction detection device includes: casing assembly 1, carrier assemblies 2, sample-adding component 3, matched reagent
Box rest area 4, PCR fluorescence detection component 5, control computer installation.The casing assembly 1 is used to accommodate the work of detection device
Main body, and protective effect is carried out to operative body.Be loaded component 3 for realizing sample, PCR reaction reagent, extract product from
It is dynamic to draw, deliver and be loaded.Carrier assemblies 2 for matched reagent box rest area 4 to be arranged, and be provided with temperature control module,
Mix module, carrier module；Temperature control module keeps suitable reaction temperature；Mix module be used for after sample-adding automatic mixing to promote
It reacts fully；Carrier module, which is used to for the PCR pipe after amplified reaction to be carried to PCR fluorescence detection component 5, carries out fluorescence inspection
It surveys.Matched reagent box rest area 4 include following region: first area be sample extraction area, for place sample container with
And cracking liquid kit, nucleic acid cleavage liquid built in liquid kit is cracked, need to only be loaded component 3 for sample to be tested such as blood etc. from sample
This container samples and then is added to cracking liquid kit, and the cracking of nucleic acid can be completed, release sample of nucleic acid completely；The
Two regions are the PCR pipe for preserving freeze-drying PCR reagent, and the PCR reagent of freezing dry powder form can be directly used in glimmering after sample-adding
Fluorescent Quantitative PCR measurement, the PCR reagent include that a plurality of types of PCR reaction buffers, archaeal dna polymerase, a variety of atopics draw
Object and probe form PCR amplification system；It is directly added into first area by being loaded component 3 into the PCR pipe of second area
It cracks nucleic acid product and removes RNA enzyme water, automatic mixing is carried out by the mixing module of the carrier assemblies 2, is finally obtained
Liquid can be directly used for fluorescence detection in PCR pipe.PCR fluorescence detection component 5 is glimmering according to the control computer installation setting
Fluorescent Quantitative PCR program carries out fluorescence detection.The control monitoring that the control computer installation is drawn, delivered, is loaded,
And carry out the setting of quantitative fluorescent PCR program.
The nucleic acid rapidly extracting detection kit includes two parts, is cracking liquid kit and PCR pipe respectively, wherein
Cracking liquid kit is placed on the first area；PCR pipe is placed in the second area.Nucleic acid is held in cracking liquid kit
Sample to be tested is added in the cracking liquid kit by lysate by sample-adding component 3, under lysate effect, can make disease
Malicious structure changes, and releases viral nucleic acid, and the lysate is without inhibition real-time fluorescence PCR reacted constituent, product
It can be directly used for subsequent PCR detection.The freezing dry powder PCR amplification system for being directly used in quantitative fluorescent PCR measurement is placed in PCR pipe
Reagent, the freezing dry powder PCR amplification system reagent are special with a variety of PCR reaction buffers, archaeal dna polymerase, a variety of reactions
Property primer, probe and freeze drying protectant be the gains that are prepared by freeze drying process of raw material.Component 3 is loaded by the firstth area
The PCR pipe is added in cracking nucleic acid product in domain, and removes RNA enzyme water to PCR pipe addition, mixes module by carrier assemblies 2
Automatic mixing, fluorescence detection can directly be executed to it by PCR fluorescence detection component 5.
As shown in Fig. 2-Fig. 3, the carrier assemblies 2 are specifically included: carrier 201, recessed cavity 202, the first heating coil
203, the second heating coil 204, third heating coil 205, the 4th heating coil 206, the first temperature inductor 207, second temperature induction
Device 208, transmission driving assembly 209, transmission sliding seat 210, the first vibrating base 211, first torsion component 212, second vibrate
Pedestal 213, second reverses component 214.
Wherein, as shown in Fig. 2, the recessed cavity 202 that the carrier 201 is arranged is put for accommodating the matched reagent box
Set area 4, matched reagent box rest area 4 is divided for first area and second area, therefore the recessed cavity 202 is also classified into
One cavity and the second cavity, wherein the size and shape of the first cavity with place to the first area sample container and split
The size shape of solution liquid kit matches, size and shape and the PCR pipe of placement to the second area of the second cavity
Size shape matches.The temperature control module includes: the first heating coil 203, the second heating coil 204, third heating coil 205,
Four heating coils 206, the first temperature inductor 207, second temperature inductor 208.Wherein, the first heating coil 203 and the second heating
Circle 204 surround the first cavity of the recessed cavity 202, for the cracking liquid kit progress to be placed in the first area
Heating, to remain suitable for the temperature of nucleic acid cleavage；First heating coil 203, the arrangement of the second about 204 heating coil, to make first
Cavity is heated evenly up and down；First heating coil 203, the second heating coil 204 are cricoid electric heating piece.Third heating coil 205,
Four heating coils 206 around the cavity body 202 the second cavity, for being added for the PCR pipe that is placed in the second area
Heat, to maintain the temperature for being suitable for amplified reaction；Third heating coil 205, the 4th heating coil 206 are also arrangement up and down, make the
Two cavity upper and lower part temperature are uniform；Also, third heating coil 205, the 4th heating coil 206 are also electric heating piece；And third
Heating coil 205, the 4th heating coil 206 are not closed annular, but respectively have an opening, and the opening of the two is right each other
Together, to allow the PCR pipe via the opening across third heating coil 205, the 4th heating coil 206, thus described in disengaging
Second cavity, to realize delivery of the PCR pipe between carrier assemblies 2 and PCR fluorescence detection component 5.The first temperature sense
Device 207 is answered to adjust first heating for monitoring the intracorporal real time temperature of the first chamber, and according to the temperature value of monitoring
The calorific value of circle 203 and the second heating coil 204, to remain suitable for the temperature of nucleic acid cleavage.Second temperature inductor 208 is used for
The real time temperature of second cavity is monitored, and third heating coil 205 and the 4th heating coil are adjusted according to the temperature value of monitoring
206 calorific value, to maintain the temperature for being suitble to amplified reaction.
As shown in figure 3, the carrier module includes transmission driving assembly 209 and transmission sliding seat 210.The PCR pipe
Be placed on the transmission sliding seat 210 in the second area, and transmit driving assembly 209 include torsion arm 209A with
And driving motor 209B, wherein transmission sliding seat 210 and the first end of torsion arm 209A are fixed, and the second of torsion arm 209A
End then connects the motor shaft of driving motor 209B.To by the rotation of the motor shaft of driving motor 209B, through torsion arm
209A is driven and the transmission sliding seat 210 is driven to slide.PCR pipe sliding is carried by transmitting sliding seat 210, it can be with
Through third heating coil 205, the 4th heating coil 206 opening and enter in second cavity, split at the second cavity
Solution nucleic acid product and the sample-adding for removing RNA enzyme water；The PCR pipe can also be carried by transmission sliding seat 210 from second chamber
Body moves at the PCR fluorescence detection component 5, carries out fluorescence detection to by the sample after amplified reaction.
The mixing module includes that the first vibrating base 211, first reverses component 212, the second vibrating base 213, second
Reverse component 214.Wherein the first vibrating base 211, first torsion component 212 is used for the intracorporal cracking liquid kit of the first chamber
Apply vibration and torsion, so that the sample that the kit is added be promoted to mix well with lysate；First vibrating base 211 by
Eccentric vibrating motor provides power to realize vibration, and the first torsion component 212 is driven by torsion motor in certain angle range
It is interior back and forth to be reversed.Second vibrating base 213 and the second torsion component 214 then drive the intracorporal PCR pipe of the second chamber to realize
Vibration and torsion, so that the PCR reagent of freezing dry powder form be promoted nucleic acid product and RNA enzyme water to be gone to mix with cracking
It is even.
Sample to be tested such as blood etc. is sampled from sample container and then is added to cracking liquid kit by sample-adding component 3, and
Cracking nucleic acid product is added into PCR pipe and removes RNA enzyme water.The sample-adding component 3 includes the machinery of tri- axial action of X-Y-Z
Arm 301 and automatic sampling gun 302.Such as Fig. 1, mechanical arm 301 divides for upper arm 301A, lower arm 301B and support rod 301C,
In, it is connected between upper arm 301A and support rod 301C by the tri- axis omnidirectional joint X-Y-Z, therefore upper arm 301A can be in X-Y-Z
Angle is adjusted in three axis directions, so that (target includes that sample holds to the target that the alignment of automatic sampling gun 302 will be aspirated and is added dropwise
Device, cracking liquid kit and PCR pipe).It is connected between lower arm 301B and upper arm 301A by unidirectional arthrodia, thus lower arm
301B can be slided up and down relative to upper arm 301A to adjust the head end height of automatic sampling gun 302, realize the automatic sampling gun
Contact between 302 and draw target is realized under contact condition and is drawn, and then moves up realization separation.
As shown in figure 4, automatic sampling gun 302 is equipped with gun platform 302A and sliding pipette tips 302B, sliding pipette tips 302B
In the lower section of gun platform 302A, and between be equipped with buffer part 302C.Buffer part 302C is mounted on gun platform 302A and sliding pipette tips 302B
Between, realize effective buffer function, when being loaded, when making 302 contact target of automatic sampling gun by mechanical arm 301,
Automatic sampling gun 302 continues traveling downwardly, and buffer part 302C plays a role at this time, enables between automatic sampling gun 302 and target
Effectively fitting.The buffer spring 302H that the buffer part 302C specifically includes guide post 302G and is set on guide post 302G, it is described
The one end guide post 302G is fixedly connected with sliding pipette tips 302B, and the other end passes through gun platform 302A and is slidably matched with it, the buffering elastic
Spring 302H is set between gun platform 302A and sliding pipette tips 302B.The sliding pipette tips 302B is equipped with back to the side gun platform 302A and fixes
Pipette 302E is installed, the fixing head 302D is connected to vacuum and drives liquid system 302F above head 302D, fixing head 302D.It is described
It includes liquid storage chamber 302I and vacuum suction syringe 302J that vacuum, which drives liquid system 302F,；When executing absorption operation, vacuum suction
Syringe 302J provides vacuum force, and liquid is pumped to liquid storage chamber 302I through fixing head 302D and is saved；Make when being added dropwise
When industry, vacuum suction syringe 302J provide thrust, and liquid is released from liquid storage chamber 302I through fixing head 302D.Pipette 302E
Through sliding sleeve 302K in conjunction with fixing head 302D, therefore pipette 302E can be realized from fixing head by moving up sliding sleeve 302K
Automatic drawing unloading on 302D.
Nucleic acid product will cracked by sample-adding component 3 and going RNA enzyme water that PCR pipe is added, and by mixing well completion
After amplified reaction, which is sent to the PCR fluorescence detection component 5 by the carrier module, by the PCR fluorescence
Detection components 5 carry out fluorescence detection according to the quantitative fluorescent PCR program of the control computer installation setting.The PCR fluorescence
Detection components 5 include the test chamber 501 that shines, the micropore closed plate made outside the test chamber 501 that shines of black light-absorbing material
502 omnidirectionals surround, these avoid the interference effect of extraneous light.There is translucency micropore 503 in micropore closed plate 502,
The optical signal of the generation in the luminous test chamber 501 is exported by the translucency micropore.Optical measuring instrument 504 is logical for incuding
Cross the light signal strength that the translucency micropore 503 exports.The optical signal of translucency micropore 503 and the optical measuring instrument 504 is defeated
Inbound port by fiber optic conduction component 505 be connected with each other, the optical measuring instrument further include optical gain converter, signal amplifier with
And analog to digital conversion circuit, optical gain converter and signal amplifier are used to convert optical signal into electric signal and be amplified,
And then will be electric signal digitising by analog to digital conversion circuit, final output quantifies table generated by signal acquisition, amplification, sampling
Show the digital signal of the light signal strength.In one-shot measurement, acquisition light signal strength is acquired repeatedly.Control computer installation
For receiving the digital signal of the transmission of optical measuring instrument 504, based on a large amount of light signal strength, generation and sample to be tested
Corresponding luminosity curve, luminosity curve indicate the relationship of light signal strength and time.And it controls computer installation also to prestore
Several standard curves, the corresponding reference value of every standard curve are stored up.Luminosity curve and standard curve are fitted, produced
Raw measurement numerical value.
As it can be seen that the present invention realizes sample from the quick full automatic working for handling, extracting detection, collocation cracking liquid kit
And PCR pipe, it can complete to detect the real-time fluorescence nucleic acid molecules of polymorphic type body fluid sample with single step, applicable sample includes blood
Liquid, serum, blood plasma, saliva, urine, tissue fluid, sperm, juice, pus and the breathing polymorphic types sample such as liquid and mucus.This hair
Bright carrier can provide the temperature suitable for nucleic acid cleavage and amplified reaction, and realize automatically and mix and convey operation；Sample-adding
Component can flexibly realize that sample is drawn and added, and have buffer structure, avoid contacting firmly and bring damage, and facilitate suction
Liquid pipe updates；Digitlization quantitative judge can be supported in fluorescence detection, with that recognition result is accurate, recognition speed is fast is excellent
Gesture.
Above embodiments are merely to illustrate the present invention, and not limitation of the present invention, the common skill in relation to technical field
Art personnel can also make a variety of changes and modification without departing from the spirit and scope of the present invention, therefore all etc.
Same technical solution also belongs to scope of the invention, and scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a kind of full-automatic nucleic acid extraction detection system characterized by comprising full-automatic PCR nucleic acid extraction detection device with
And nucleic acid rapidly extracting detection kit；Wherein
The full-automatic PCR nucleic acid extraction detection device includes: that casing assembly, carrier assemblies, sample-adding component, matched reagent box are put
Set area, PCR fluorescence detection component, control computer installation；The casing assembly is used to accommodate the operative body of detection device, and
Protective effect is carried out to operative body；Be loaded component for realizing sample, PCR reaction reagent, extract product automatic sucking,
Delivery and sample-adding；Carrier assemblies for matched reagent box rest area to be arranged, and be provided with temperature control module, mix module,
Carrier module；Temperature control module keeps suitable reaction temperature；Mixing module is used for the automatic mixing after sample-adding and is filled with promoting to react
Point；Carrier module, which is used to for the PCR pipe after amplified reaction to be carried to PCR fluorescence detection component, carries out fluorescence detection；Mating examination
The first area of agent box rest area is sample extraction area, for placing sample container and cracking liquid kit；Matched reagent box
The second area of rest area is for placing PCR pipe；The fluorescence that PCR fluorescence detection component is arranged according to the control computer installation
Quantitative PCR program carries out fluorescence detection；The control monitoring that the control computer installation is drawn, delivered, is loaded, with
And carry out the setting of quantitative fluorescent PCR program；
The nucleic acid rapidly extracting detection kit includes cracking liquid kit and PCR pipe, wherein cracking liquid kit is placed
In the first area；PCR pipe is placed in the second area；Nucleic acid cleavage liquid is held in cracking liquid kit, by sample-adding group
Sample to be tested is added in the cracking liquid kit by part, releases viral nucleic acid under lysate effect；It is described
The freezing dry powder PCR amplification system reagent for being directly used in quantitative fluorescent PCR measurement is placed in PCR pipe, the freezing dry powder PCR expands
Increasing system reagent is using PCR reaction buffer, archaeal dna polymerase, atopic primer, probe and freeze drying protectant as raw material
The gains prepared by freeze drying process.
2. full-automatic nucleic acid extraction detection system according to claim 1, which is characterized in that the carrier assemblies specifically wrap
It includes: carrier, recessed cavity；The recessed cavity of the carrier setting is for accommodating matched reagent box rest area；It is described
Recessed cavity is divided into the first cavity and the second cavity, wherein the size and shape of the first cavity and placement to the first area
Sample container and the size shape for cracking liquid kit match, size and shape and the placement to described second of the second cavity
The size shape of the PCR pipe in region matches.
3. full-automatic nucleic acid extraction detection system according to claim 2, which is characterized in that the temperature control of the carrier assemblies
Module includes: the first heating coil, the second heating coil, third heating coil, the 4th heating coil, the first temperature inductor, second temperature
Inductor；Wherein, the first heating coil and the second heating coil surround the first cavity of the recessed cavity, for described to be placed in
The cracking liquid kit of first area is heated, to remain suitable for the temperature of nucleic acid cleavage；First heating coil, the second heating coil
Arrangement up and down, so that the first cavity be made to be heated evenly up and down；First heating coil, the second heating coil are cricoid electric heating piece；The
Three heating coils, the 4th heating coil around the cavity body the second cavity, for for be placed in the PCR pipe of the second area into
Row heating, to maintain the temperature for being suitable for amplified reaction；Third heating coil, the 4th heating coil are arranged up and down, make the second cavity
Upper and lower part temperature is uniform；Third heating coil, the 4th heating coil are also electric heating piece；And third heating coil, the 4th heating coil
Respectively there is an opening, the opening of the two is in alignment with each other, so that the PCR pipe be allowed to heat via the opening across third
Circle, the 4th heating coil, to pass in and out second cavity.
4. full-automatic nucleic acid extraction detection system according to claim 3, which is characterized in that the carrier module includes:
Transmit driving assembly and transmission sliding seat；The PCR pipe is placed in the second area on the transmission sliding seat, and
And transmission driving assembly includes torsion arm and driving motor, wherein the first end of transmission sliding seat and torsion arm is fixed, and
The second end of torsion arm then connects the motor shaft of driving motor；To by the rotation of the motor shaft of driving motor, through reversing
Arm is driven and the transmission sliding seat is driven to slide；The PCR pipe sliding is carried by transmission sliding seat, can be heated through third
Circle, the 4th heating coil opening and enter in second cavity, cracking is carried out at the second cavity and nucleic acid product and is gone
The sample-adding of RNA enzyme water；The PCR pipe can also be carried by transmission sliding seat and move to the PCR fluorescence from second cavity
At detection components, fluorescence detection is carried out to by the sample after amplified reaction.
5. full-automatic nucleic acid extraction detection system according to claim 4, which is characterized in that the mixing module includes the
One vibrating base, the first torsion component, the second vibrating base, the second torsion component；Wherein the first vibrating base, the first torsion group
Part applies vibration and torsion for cracking liquid kit intracorporal to the first chamber, thus promote to be added the kit sample and
Lysate mixes well；Second vibrating base and the second torsion component then drive the intracorporal PCR pipe of the second chamber realize vibration with
And torsion, so that the PCR reagent of freezing dry powder form be promoted nucleic acid product and RNA enzyme water to be gone to be uniformly mixed with cracking.
6. full-automatic nucleic acid extraction detection system according to claim 5, which is characterized in that the sample-adding component includes X-
The mechanical arm and automatic sampling gun of tri- axial action of Y-Z；The mechanical arm is for carrying the automatic sampling gun and suction mesh
Tag splice touching；The automatic sampling gun is used to realize under contact condition and draw, and is added dropwise under contactless state.
7. full-automatic nucleic acid extraction detection system according to claim 6, which is characterized in that the mechanical arm is divided into
Arm, lower arm and support rod, wherein connected between upper arm and support rod by the tri- axis omnidirectional joint X-Y-Z, upper arm can be in X-
Angle is adjusted in tri- axis direction of Y-Z, so that automatic sampling gun is directed at the target that aspirate and be added dropwise；Between the lower arm and upper arm
It is connected by unidirectional arthrodia, so that lower arm can be slided up and down relative to upper arm to adjust the head end of automatic sampling gun height
Degree.
8. full-automatic nucleic acid extraction detection system according to claim 7, which is characterized in that the automatic sampling gun includes
Gun platform and sliding pipette tips, the sliding pipette tips are located at the lower section of gun platform, and are equipped with buffer part between gun platform and sliding pipette tips, realize
Effective buffer function.
9. full-automatic nucleic acid extraction detection system according to claim 8, which is characterized in that the sliding pipette tips back
Fixing head is equipped with to gun platform side, pipette is installed above fixing head；The fixing head is connected to vacuum and drives liquid system；It is described true
It includes liquid storage chamber and vacuum suction syringe that sky, which drives liquid system,.
10. full-automatic nucleic acid extraction detection system according to claim 9, which is characterized in that the PCR fluorescence detection group
Part includes the test chamber that shines, and the micropore closed plate omnidirectional of institute made outside the test chamber that shines of black light-absorbing material surrounds, micro-
There is translucency micropore in the closed plate of hole, believed by the light that the translucency micropore exports the generation in the luminous test chamber
Number；Optical measuring instrument is used to incude the light signal strength exported by the translucency micropore；Translucency micropore and the light measurement
The optical signal input mouth of instrument is connected with each other by fiber optic conduction component.
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