ZA202210414B - Sample treatment method of in-situ in vitro cultured cells for tem observation - Google Patents
Sample treatment method of in-situ in vitro cultured cells for tem observationInfo
- Publication number
- ZA202210414B ZA202210414B ZA2022/10414A ZA202210414A ZA202210414B ZA 202210414 B ZA202210414 B ZA 202210414B ZA 2022/10414 A ZA2022/10414 A ZA 2022/10414A ZA 202210414 A ZA202210414 A ZA 202210414A ZA 202210414 B ZA202210414 B ZA 202210414B
- Authority
- ZA
- South Africa
- Prior art keywords
- situ
- treatment method
- cultured cells
- tem observation
- sample treatment
- Prior art date
Links
- 210000004748 cultured cell Anatomy 0.000 title 1
- 238000000338 in vitro Methods 0.000 title 1
- 238000011065 in-situ storage Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111003683.2A CN113654863A (en) | 2021-08-30 | 2021-08-30 | Sample processing method of in-situ in-vitro cultured cells for transmission electron microscope observation |
| PCT/CN2022/111315 WO2023029909A1 (en) | 2021-08-30 | 2022-08-10 | Sample treatment method for in-situ in vitro culture cells for observation by transmission electron microscopy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA202210414B true ZA202210414B (en) | 2023-04-26 |
Family
ID=78493176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2022/10414A ZA202210414B (en) | 2021-08-30 | 2022-09-20 | Sample treatment method of in-situ in vitro cultured cells for tem observation |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN113654863A (en) |
| WO (1) | WO2023029909A1 (en) |
| ZA (1) | ZA202210414B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113654863A (en) * | 2021-08-30 | 2021-11-16 | 南通大学 | Sample processing method of in-situ in-vitro cultured cells for transmission electron microscope observation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007309872A (en) * | 2006-05-22 | 2007-11-29 | Nitto Denko Corp | Method of preparing sample for transmission electron microscope |
| CN109781493A (en) * | 2018-12-26 | 2019-05-21 | 华中科技大学苏州脑空间信息研究院 | It is a kind of can long-time continuous fine upper cut imaging biological tissue's hydrogel embedding method |
| CN110082380B (en) * | 2019-05-13 | 2022-04-08 | 曾琪琪 | Method for in-situ observation of intercellular cell junction of single layer by transmission electron microscope |
| CN113654863A (en) * | 2021-08-30 | 2021-11-16 | 南通大学 | Sample processing method of in-situ in-vitro cultured cells for transmission electron microscope observation |
-
2021
- 2021-08-30 CN CN202111003683.2A patent/CN113654863A/en active Pending
-
2022
- 2022-08-10 WO PCT/CN2022/111315 patent/WO2023029909A1/en active Application Filing
- 2022-09-20 ZA ZA2022/10414A patent/ZA202210414B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN113654863A (en) | 2021-11-16 |
| WO2023029909A1 (en) | 2023-03-09 |
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