ZA200104414B

ZA200104414B - Compounds and methods for treatment and diagnosis of chlamydial infection.

Compounds and methods for treatment and diagnosis of chlamydial infection. Download PDF

Info

Publication number: ZA200104414B
Authority: ZA; South Africa
Prior art keywords: seq; sequence; polypeptide; polynucleotide; cells
Prior art date: 1998-12-08

Application number

ZA200104414A

Other languages

English (en)

Inventor

Peter Probst

Yasir A W Skeiky

Shyian Jen

Ajay Bhatia

Steven P Fling

Erica Jean Stromberg

Original Assignee

Corixa Corp

Priority date

1998-12-08

Filing date

2001-05-29

Publication date

2002-08-29

2001-05-29 Application filed by Corixa Corp filed Critical Corixa Corp

2002-08-29 Publication of ZA200104414B publication Critical patent/ZA200104414B/en

Links

238000000034 method Methods 0.000 title claims description 141
238000011282 treatment Methods 0.000 title claims description 25
206010061041 Chlamydial infection Diseases 0.000 title description 23
201000000902 chlamydia Diseases 0.000 title description 22
208000012538 chlamydia trachomatis infectious disease Diseases 0.000 title description 22
150000001875 compounds Chemical class 0.000 title description 16
238000003745 diagnosis Methods 0.000 title description 4
108090000765 processed proteins & peptides Proteins 0.000 claims description 401
102000004196 processed proteins & peptides Human genes 0.000 claims description 272
229920001184 polypeptide Polymers 0.000 claims description 246
108090000623 proteins and genes Proteins 0.000 claims description 222
125000003275 alpha amino acid group Chemical group 0.000 claims description 196
239000000427 antigen Substances 0.000 claims description 165
108091007433 antigens Proteins 0.000 claims description 165
102000036639 antigens Human genes 0.000 claims description 165
102000004169 proteins and genes Human genes 0.000 claims description 136
210000001744 T-lymphocyte Anatomy 0.000 claims description 133
102000040430 polynucleotide Human genes 0.000 claims description 129
108091033319 polynucleotide Proteins 0.000 claims description 129
239000002157 polynucleotide Substances 0.000 claims description 129
210000004027 cell Anatomy 0.000 claims description 126
241000606161 Chlamydia Species 0.000 claims description 103
239000013598 vector Substances 0.000 claims description 64
229960005486 vaccine Drugs 0.000 claims description 55
108020004414 DNA Proteins 0.000 claims description 52
239000000523 sample Substances 0.000 claims description 51
230000014509 gene expression Effects 0.000 claims description 48
239000000203 mixture Substances 0.000 claims description 48
238000010367 cloning Methods 0.000 claims description 44
210000004443 dendritic cell Anatomy 0.000 claims description 44
230000002163 immunogen Effects 0.000 claims description 44
150000001413 amino acids Chemical class 0.000 claims description 35
102000037865 fusion proteins Human genes 0.000 claims description 35
108020001507 fusion proteins Proteins 0.000 claims description 35
208000007190 Chlamydia Infections Diseases 0.000 claims description 33
230000027455 binding Effects 0.000 claims description 31
239000012634 fragment Substances 0.000 claims description 31
208000015181 infectious disease Diseases 0.000 claims description 31
239000002773 nucleotide Substances 0.000 claims description 31
125000003729 nucleotide group Chemical group 0.000 claims description 31
238000003752 polymerase chain reaction Methods 0.000 claims description 31
239000012472 biological sample Substances 0.000 claims description 29
210000000612 antigen-presenting cell Anatomy 0.000 claims description 28
230000000295 complement effect Effects 0.000 claims description 28
239000008194 pharmaceutical composition Substances 0.000 claims description 28
239000002671 adjuvant Substances 0.000 claims description 27
239000000126 substance Substances 0.000 claims description 27
239000003153 chemical reaction reagent Substances 0.000 claims description 26
238000001514 detection method Methods 0.000 claims description 25
239000011230 binding agent Substances 0.000 claims description 23
239000013604 expression vector Substances 0.000 claims description 23
210000001616 monocyte Anatomy 0.000 claims description 19
125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 19
238000004519 manufacturing process Methods 0.000 claims description 18
239000007787 solid Substances 0.000 claims description 18
229960001438 immunostimulant agent Drugs 0.000 claims description 17
239000003022 immunostimulating agent Substances 0.000 claims description 17
230000003308 immunostimulating effect Effects 0.000 claims description 17
210000002966 serum Anatomy 0.000 claims description 17
125000006853 reporter group Chemical group 0.000 claims description 16
239000003155 DNA primer Substances 0.000 claims description 14
239000003814 drug Substances 0.000 claims description 13
230000001681 protective effect Effects 0.000 claims description 13
102000053602 DNA Human genes 0.000 claims description 12
241000588724 Escherichia coli Species 0.000 claims description 11
239000002751 oligonucleotide probe Substances 0.000 claims description 11
108020005187 Oligonucleotide Probes Proteins 0.000 claims description 10
230000036039 immunity Effects 0.000 claims description 10
238000009007 Diagnostic Kit Methods 0.000 claims description 9
210000004369 blood Anatomy 0.000 claims description 9
239000008280 blood Substances 0.000 claims description 9
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
102000004190 Enzymes Human genes 0.000 claims description 6
108090000790 Enzymes Proteins 0.000 claims description 6
230000001939 inductive effect Effects 0.000 claims description 6
239000002253 acid Substances 0.000 claims description 5
239000003623 enhancer Substances 0.000 claims description 5
210000002540 macrophage Anatomy 0.000 claims description 5
210000004962 mammalian cell Anatomy 0.000 claims description 5
210000002381 plasma Anatomy 0.000 claims description 5
210000002700 urine Anatomy 0.000 claims description 5
206010036790 Productive cough Diseases 0.000 claims description 4
210000003719 b-lymphocyte Anatomy 0.000 claims description 4
210000001175 cerebrospinal fluid Anatomy 0.000 claims description 4
210000003296 saliva Anatomy 0.000 claims description 4
210000003802 sputum Anatomy 0.000 claims description 4
208000024794 sputum Diseases 0.000 claims description 4
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
229960002685 biotin Drugs 0.000 claims description 3
235000020958 biotin Nutrition 0.000 claims description 3
239000011616 biotin Substances 0.000 claims description 3
210000000130 stem cell Anatomy 0.000 claims description 3
108090001090 Lectins Proteins 0.000 claims description 2
102000004856 Lectins Human genes 0.000 claims description 2
101710120037 Toxin CcdB Proteins 0.000 claims description 2
229940072221 immunoglobulins Drugs 0.000 claims description 2
239000002523 lectin Substances 0.000 claims description 2
238000003259 recombinant expression Methods 0.000 claims description 2
210000004976 peripheral blood cell Anatomy 0.000 claims 5
210000001266 CD8-positive T-lymphocyte Anatomy 0.000 claims 2
230000004888 barrier function Effects 0.000 claims 2
239000002245 particle Substances 0.000 claims 1
210000005253 yeast cell Anatomy 0.000 claims 1
241000606153 Chlamydia trachomatis Species 0.000 description 337
108091028043 Nucleic acid sequence Proteins 0.000 description 182
235000018102 proteins Nutrition 0.000 description 120
108700026244 Open Reading Frames Proteins 0.000 description 93
239000013615 primer Substances 0.000 description 73
241001647372 Chlamydia pneumoniae Species 0.000 description 62
229940038705 chlamydia trachomatis Drugs 0.000 description 39
235000001014 amino acid Nutrition 0.000 description 38
238000003556 assay Methods 0.000 description 36
229940024606 amino acid Drugs 0.000 description 35
102100035954 Choline transporter-like protein 2 Human genes 0.000 description 33
101000948115 Homo sapiens Choline transporter-like protein 2 Proteins 0.000 description 33
206010035664 Pneumonia Diseases 0.000 description 31
241000498849 Chlamydiales Species 0.000 description 28
230000004044 response Effects 0.000 description 28
101150106828 pmpD gene Proteins 0.000 description 25
230000009696 proliferative response Effects 0.000 description 25
238000002965 ELISA Methods 0.000 description 23
241000699670 Mus sp. Species 0.000 description 23
230000028993 immune response Effects 0.000 description 22
125000005647 linker group Chemical group 0.000 description 22
230000036961 partial effect Effects 0.000 description 22
101150061263 tct-1 gene Proteins 0.000 description 22
101150050161 pmpC gene Proteins 0.000 description 20
239000003795 chemical substances by application Substances 0.000 description 19
210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 19
239000002299 complementary DNA Substances 0.000 description 18
230000004927 fusion Effects 0.000 description 18
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 17
241001465754 Metazoa Species 0.000 description 17
206010061218 Inflammation Diseases 0.000 description 16
108010076504 Protein Sorting Signals Proteins 0.000 description 16
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 16
230000003053 immunization Effects 0.000 description 16
238000002649 immunization Methods 0.000 description 16
230000004054 inflammatory process Effects 0.000 description 16
239000002953 phosphate buffered saline Substances 0.000 description 16
101150059608 pmpE gene Proteins 0.000 description 16
102000004127 Cytokines Human genes 0.000 description 14
108090000695 Cytokines Proteins 0.000 description 14
101150053615 pmpG gene Proteins 0.000 description 14
230000004936 stimulating effect Effects 0.000 description 14
210000004899 c-terminal region Anatomy 0.000 description 13
238000001727 in vivo Methods 0.000 description 13
238000007792 addition Methods 0.000 description 12
239000012636 effector Substances 0.000 description 12
238000002474 experimental method Methods 0.000 description 12
230000001900 immune effect Effects 0.000 description 12
230000006698 induction Effects 0.000 description 12
230000035755 proliferation Effects 0.000 description 12
101150077194 CAP1 gene Proteins 0.000 description 11
101100245221 Mus musculus Prss8 gene Proteins 0.000 description 11
108091034117 Oligonucleotide Proteins 0.000 description 11
230000005867 T cell response Effects 0.000 description 11
230000003321 amplification Effects 0.000 description 11
239000011651 chromium Substances 0.000 description 11
238000011081 inoculation Methods 0.000 description 11
239000002609 medium Substances 0.000 description 11
238000003199 nucleic acid amplification method Methods 0.000 description 11
210000003101 oviduct Anatomy 0.000 description 11
238000000746 purification Methods 0.000 description 11
210000004989 spleen cell Anatomy 0.000 description 11
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 10
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 10
238000009472 formulation Methods 0.000 description 10
238000011534 incubation Methods 0.000 description 10
239000012528 membrane Substances 0.000 description 10
210000004379 membrane Anatomy 0.000 description 10
210000001672 ovary Anatomy 0.000 description 10
230000009257 reactivity Effects 0.000 description 10
101100462201 Mus musculus Opn4 gene Proteins 0.000 description 9
125000000539 amino acid group Chemical group 0.000 description 9
230000009089 cytolysis Effects 0.000 description 9
238000012217 deletion Methods 0.000 description 9
230000037430 deletion Effects 0.000 description 9
238000009169 immunotherapy Methods 0.000 description 9
238000010561 standard procedure Methods 0.000 description 9
238000006467 substitution reaction Methods 0.000 description 9
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 8
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 8
238000004458 analytical method Methods 0.000 description 8
230000000692 anti-sense effect Effects 0.000 description 8
230000001580 bacterial effect Effects 0.000 description 8
238000013507 mapping Methods 0.000 description 8
239000004005 microsphere Substances 0.000 description 8
238000002360 preparation method Methods 0.000 description 8
230000002441 reversible effect Effects 0.000 description 8
238000012216 screening Methods 0.000 description 8
230000000638 stimulation Effects 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
238000011735 C3H mouse Methods 0.000 description 7
101100494773 Caenorhabditis elegans ctl-2 gene Proteins 0.000 description 7
102000002278 Ribosomal Proteins Human genes 0.000 description 7
108010000605 Ribosomal Proteins Proteins 0.000 description 7
241000700605 Viruses Species 0.000 description 7
230000015572 biosynthetic process Effects 0.000 description 7
239000000969 carrier Substances 0.000 description 7
201000010099 disease Diseases 0.000 description 7
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
238000012986 modification Methods 0.000 description 7
230000004048 modification Effects 0.000 description 7
239000013612 plasmid Substances 0.000 description 7
-1 rRNA Proteins 0.000 description 7
238000012360 testing method Methods 0.000 description 7
241000894006 Bacteria Species 0.000 description 6
201000005019 Chlamydia pneumonia Diseases 0.000 description 6
108020004705 Codon Proteins 0.000 description 6
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 6
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 6
108010028127 Dihydrolipoamide Dehydrogenase Proteins 0.000 description 6
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 6
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 6
108091081024 Start codon Proteins 0.000 description 6
230000006052 T cell proliferation Effects 0.000 description 6
102100036011 T-cell surface glycoprotein CD4 Human genes 0.000 description 6
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
238000001042 affinity chromatography Methods 0.000 description 6
239000011324 bead Substances 0.000 description 6
230000005847 immunogenicity Effects 0.000 description 6
238000003780 insertion Methods 0.000 description 6
230000037431 insertion Effects 0.000 description 6
239000002502 liposome Substances 0.000 description 6
239000013642 negative control Substances 0.000 description 6
150000007523 nucleic acids Chemical class 0.000 description 6
208000030773 pneumonia caused by chlamydia Diseases 0.000 description 6
230000001177 retroviral effect Effects 0.000 description 6
238000003786 synthesis reaction Methods 0.000 description 6
238000011144 upstream manufacturing Methods 0.000 description 6
102000009016 Cholera Toxin Human genes 0.000 description 5
108010049048 Cholera Toxin Proteins 0.000 description 5
238000012408 PCR amplification Methods 0.000 description 5
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
238000013459 approach Methods 0.000 description 5
239000000872 buffer Substances 0.000 description 5
238000001516 cell proliferation assay Methods 0.000 description 5
210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 5
238000002405 diagnostic procedure Methods 0.000 description 5
101150052825 dnaK gene Proteins 0.000 description 5
229940079593 drug Drugs 0.000 description 5
238000005516 engineering process Methods 0.000 description 5
229940088598 enzyme Drugs 0.000 description 5
238000001400 expression cloning Methods 0.000 description 5
238000003018 immunoassay Methods 0.000 description 5
229940127121 immunoconjugate Drugs 0.000 description 5
238000000338 in vitro Methods 0.000 description 5
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 5
108020004999 messenger RNA Proteins 0.000 description 5
102000039446 nucleic acids Human genes 0.000 description 5
108020004707 nucleic acids Proteins 0.000 description 5
108030002458 peroxiredoxin Proteins 0.000 description 5
101150067195 pmpB gene Proteins 0.000 description 5
210000005000 reproductive tract Anatomy 0.000 description 5
210000003705 ribosome Anatomy 0.000 description 5
210000004988 splenocyte Anatomy 0.000 description 5
238000007920 subcutaneous administration Methods 0.000 description 5
239000006228 supernatant Substances 0.000 description 5
230000001225 therapeutic effect Effects 0.000 description 5
238000001890 transfection Methods 0.000 description 5
238000013519 translation Methods 0.000 description 5
108700028369 Alleles Proteins 0.000 description 4
101100438368 Caenorhabditis elegans cap-1 gene Proteins 0.000 description 4
241001647378 Chlamydia psittaci Species 0.000 description 4
206010008631 Cholera Diseases 0.000 description 4
108091060211 Expressed sequence tag Proteins 0.000 description 4
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 4
108010002350 Interleukin-2 Proteins 0.000 description 4
102000000588 Interleukin-2 Human genes 0.000 description 4
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 4
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 4
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 4
241001529936 Murinae Species 0.000 description 4
206010028980 Neoplasm Diseases 0.000 description 4
241000283973 Oryctolagus cuniculus Species 0.000 description 4
208000029082 Pelvic Inflammatory Disease Diseases 0.000 description 4
101100428373 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) POR1 gene Proteins 0.000 description 4
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 4
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
229960003767 alanine Drugs 0.000 description 4
235000004279 alanine Nutrition 0.000 description 4
230000000890 antigenic effect Effects 0.000 description 4
201000011510 cancer Diseases 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
239000012228 culture supernatant Substances 0.000 description 4
230000004069 differentiation Effects 0.000 description 4
238000010790 dilution Methods 0.000 description 4
239000012895 dilution Substances 0.000 description 4
230000000694 effects Effects 0.000 description 4
235000013861 fat-free Nutrition 0.000 description 4
230000028996 humoral immune response Effects 0.000 description 4
238000011065 in-situ storage Methods 0.000 description 4
230000002458 infectious effect Effects 0.000 description 4
238000002347 injection Methods 0.000 description 4
239000007924 injection Substances 0.000 description 4
HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 4
101150087557 omcB gene Proteins 0.000 description 4
210000000056 organ Anatomy 0.000 description 4
235000008476 powdered milk Nutrition 0.000 description 4
239000002987 primer (paints) Substances 0.000 description 4
230000008569 process Effects 0.000 description 4
239000000047 product Substances 0.000 description 4
238000012163 sequencing technique Methods 0.000 description 4
229940124597 therapeutic agent Drugs 0.000 description 4
229940104230 thymidine Drugs 0.000 description 4
239000003053 toxin Substances 0.000 description 4
231100000765 toxin Toxicity 0.000 description 4
108700012359 toxins Proteins 0.000 description 4
238000013518 transcription Methods 0.000 description 4
230000035897 transcription Effects 0.000 description 4
241000701161 unidentified adenovirus Species 0.000 description 4
208000000143 urethritis Diseases 0.000 description 4
239000003981 vehicle Substances 0.000 description 4
230000003612 virological effect Effects 0.000 description 4
IQFYYKKMVGJFEH-OFKYTIFKSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(tritiooxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO[3H])O[C@H]1N1C(=O)NC(=O)C(C)=C1 IQFYYKKMVGJFEH-OFKYTIFKSA-N 0.000 description 3
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
101000768957 Acholeplasma phage L2 Uncharacterized 37.2 kDa protein Proteins 0.000 description 3
101000823746 Acidianus ambivalens Uncharacterized 17.7 kDa protein in bps2 3'region Proteins 0.000 description 3
101000916369 Acidianus ambivalens Uncharacterized protein in sor 5'region Proteins 0.000 description 3
101000769342 Acinetobacter guillouiae Uncharacterized protein in rpoN-murA intergenic region Proteins 0.000 description 3
101000823696 Actinobacillus pleuropneumoniae Uncharacterized glycosyltransferase in aroQ 3'region Proteins 0.000 description 3
229920000936 Agarose Polymers 0.000 description 3
101000786513 Agrobacterium tumefaciens (strain 15955) Uncharacterized protein outside the virF region Proteins 0.000 description 3
101000618005 Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) Uncharacterized protein BpOF4_00885 Proteins 0.000 description 3
102100020724 Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 Human genes 0.000 description 3
101000967489 Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB 13405 / ORS 571) Uncharacterized protein AZC_3924 Proteins 0.000 description 3
101000823761 Bacillus licheniformis Uncharacterized 9.4 kDa protein in flaL 3'region Proteins 0.000 description 3
101000819719 Bacillus methanolicus Uncharacterized N-acetyltransferase in lysA 3'region Proteins 0.000 description 3
101000789586 Bacillus subtilis (strain 168) UPF0702 transmembrane protein YkjA Proteins 0.000 description 3
101000792624 Bacillus subtilis (strain 168) Uncharacterized protein YbxH Proteins 0.000 description 3
101000790792 Bacillus subtilis (strain 168) Uncharacterized protein YckC Proteins 0.000 description 3
101000819705 Bacillus subtilis (strain 168) Uncharacterized protein YlxR Proteins 0.000 description 3
101000948218 Bacillus subtilis (strain 168) Uncharacterized protein YtxJ Proteins 0.000 description 3
101000718627 Bacillus thuringiensis subsp. kurstaki Putative RNA polymerase sigma-G factor Proteins 0.000 description 3
101000641200 Bombyx mori densovirus Putative non-structural protein Proteins 0.000 description 3
108091003079 Bovine Serum Albumin Proteins 0.000 description 3
241000283707 Capra Species 0.000 description 3
241001185363 Chlamydiae Species 0.000 description 3
VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 3
101000947633 Claviceps purpurea Uncharacterized 13.8 kDa protein Proteins 0.000 description 3
108091026890 Coding region Proteins 0.000 description 3
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 3
102100037840 Dehydrogenase/reductase SDR family member 2, mitochondrial Human genes 0.000 description 3
238000011510 Elispot assay Methods 0.000 description 3
102100031780 Endonuclease Human genes 0.000 description 3
101000948901 Enterobacteria phage T4 Uncharacterized 16.0 kDa protein in segB-ipI intergenic region Proteins 0.000 description 3
101000805958 Equine herpesvirus 4 (strain 1942) Virion protein US10 homolog Proteins 0.000 description 3
101000790442 Escherichia coli Insertion element IS2 uncharacterized 11.1 kDa protein Proteins 0.000 description 3
101000788354 Escherichia phage P2 Uncharacterized 8.2 kDa protein in gpA 5'region Proteins 0.000 description 3
101000770304 Frankia alni UPF0460 protein in nifX-nifW intergenic region Proteins 0.000 description 3
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 3
229930182566 Gentamicin Natural products 0.000 description 3
101000797344 Geobacillus stearothermophilus Putative tRNA (cytidine(34)-2'-O)-methyltransferase Proteins 0.000 description 3
101000748410 Geobacillus stearothermophilus Uncharacterized protein in fumA 3'region Proteins 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
239000004471 Glycine Substances 0.000 description 3
108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 3
102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
101000772675 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) UPF0438 protein HI_0847 Proteins 0.000 description 3
101000631019 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Uncharacterized protein HI_0350 Proteins 0.000 description 3
101000768938 Haemophilus phage HP1 (strain HP1c1) Uncharacterized 8.9 kDa protein in int-C1 intergenic region Proteins 0.000 description 3
101000785414 Homo sapiens Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 Proteins 0.000 description 3
108010001336 Horseradish Peroxidase Proteins 0.000 description 3
108060003951 Immunoglobulin Proteins 0.000 description 3
102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
108090000978 Interleukin-4 Proteins 0.000 description 3
108091092195 Intron Proteins 0.000 description 3
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical class [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
101000782488 Junonia coenia densovirus (isolate pBRJ/1990) Putative non-structural protein NS2 Proteins 0.000 description 3
101000811523 Klebsiella pneumoniae Uncharacterized 55.8 kDa protein in cps region Proteins 0.000 description 3
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 3
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
101000818409 Lactococcus lactis subsp. lactis Uncharacterized HTH-type transcriptional regulator in lacX 3'region Proteins 0.000 description 3
101710149059 Large cysteine-rich periplasmic protein OmcB Proteins 0.000 description 3
101000878851 Leptolyngbya boryana Putative Fe(2+) transport protein A Proteins 0.000 description 3
229930195725 Mannitol Natural products 0.000 description 3
108010052285 Membrane Proteins Proteins 0.000 description 3
101000758828 Methanosarcina barkeri (strain Fusaro / DSM 804) Uncharacterized protein Mbar_A1602 Proteins 0.000 description 3
101001122401 Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) Non-structural protein ORF3 Proteins 0.000 description 3
241000699666 Mus <mouse, genus> Species 0.000 description 3
101001055788 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) Pentapeptide repeat protein MfpA Proteins 0.000 description 3
101000740670 Orgyia pseudotsugata multicapsid polyhedrosis virus Protein C42 Proteins 0.000 description 3
101000769182 Photorhabdus luminescens Uncharacterized protein in pnp 3'region Proteins 0.000 description 3
206010035226 Plasma cell myeloma Diseases 0.000 description 3
206010035673 Pneumonia chlamydial Diseases 0.000 description 3
239000004365 Protease Substances 0.000 description 3
101710188053 Protein D Proteins 0.000 description 3
101000961392 Pseudescherichia vulneris Uncharacterized 29.9 kDa protein in crtE 3'region Proteins 0.000 description 3
101000731030 Pseudomonas oleovorans Poly(3-hydroxyalkanoate) polymerase 2 Proteins 0.000 description 3
101001065485 Pseudomonas putida Probable fatty acid methyltransferase Proteins 0.000 description 3
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
108020004511 Recombinant DNA Proteins 0.000 description 3
101710132893 Resolvase Proteins 0.000 description 3
101000711023 Rhizobium leguminosarum bv. trifolii Uncharacterized protein in tfuA 3'region Proteins 0.000 description 3
101000948156 Rhodococcus erythropolis Uncharacterized 47.3 kDa protein in thcA 5'region Proteins 0.000 description 3
101000917565 Rhodococcus fascians Uncharacterized 33.6 kDa protein in fasciation locus Proteins 0.000 description 3
108090000792 Ribosomal protein L1 Proteins 0.000 description 3
101000790284 Saimiriine herpesvirus 2 (strain 488) Uncharacterized 9.5 kDa protein in DHFR 3'region Proteins 0.000 description 3
101000936719 Streptococcus gordonii Accessory Sec system protein Asp3 Proteins 0.000 description 3
101000788499 Streptomyces coelicolor Uncharacterized oxidoreductase in mprA 5'region Proteins 0.000 description 3
101001102841 Streptomyces griseus Purine nucleoside phosphorylase ORF3 Proteins 0.000 description 3
101000708557 Streptomyces lincolnensis Uncharacterized 17.2 kDa protein in melC2-rnhH intergenic region Proteins 0.000 description 3
229930006000 Sucrose Natural products 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
102100034922 T-cell surface glycoprotein CD8 alpha chain Human genes 0.000 description 3
108700012920 TNF Proteins 0.000 description 3
101150109085 TSA gene Proteins 0.000 description 3
101000649826 Thermotoga neapolitana Putative anti-sigma factor antagonist TM1081 homolog Proteins 0.000 description 3
YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 3
101710117021 Tyrosine-protein phosphatase YopH Proteins 0.000 description 3
206010046865 Vaccinia virus infection Diseases 0.000 description 3
101000827562 Vibrio alginolyticus Uncharacterized protein in proC 3'region Proteins 0.000 description 3
101000778915 Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) Uncharacterized membrane protein VP2115 Proteins 0.000 description 3
150000007513 acids Chemical class 0.000 description 3
230000004913 activation Effects 0.000 description 3
230000008901 benefit Effects 0.000 description 3
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
229940098773 bovine serum albumin Drugs 0.000 description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
238000012512 characterization method Methods 0.000 description 3
229910052804 chromium Inorganic materials 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
230000003292 diminished effect Effects 0.000 description 3
238000003114 enzyme-linked immunosorbent spot assay Methods 0.000 description 3
125000000524 functional group Chemical group 0.000 description 3
239000007789 gas Substances 0.000 description 3
210000004392 genitalia Anatomy 0.000 description 3
229960002518 gentamicin Drugs 0.000 description 3
239000008103 glucose Substances 0.000 description 3
150000004676 glycans Chemical class 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
238000009396 hybridization Methods 0.000 description 3
210000004408 hybridoma Anatomy 0.000 description 3
102000018358 immunoglobulin Human genes 0.000 description 3
238000002513 implantation Methods 0.000 description 3
230000001976 improved effect Effects 0.000 description 3
230000001965 increasing effect Effects 0.000 description 3
230000003834 intracellular effect Effects 0.000 description 3
238000007918 intramuscular administration Methods 0.000 description 3
238000001990 intravenous administration Methods 0.000 description 3
239000003446 ligand Substances 0.000 description 3
235000010355 mannitol Nutrition 0.000 description 3
239000000594 mannitol Substances 0.000 description 3
239000011159 matrix material Substances 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
229930182817 methionine Natural products 0.000 description 3
229940035032 monophosphoryl lipid a Drugs 0.000 description 3
238000002703 mutagenesis Methods 0.000 description 3
231100000350 mutagenesis Toxicity 0.000 description 3
201000000050 myeloid neoplasm Diseases 0.000 description 3
210000005259 peripheral blood Anatomy 0.000 description 3
239000011886 peripheral blood Substances 0.000 description 3
229920001282 polysaccharide Polymers 0.000 description 3
239000005017 polysaccharide Substances 0.000 description 3
230000003389 potentiating effect Effects 0.000 description 3
229960003387 progesterone Drugs 0.000 description 3
239000000186 progesterone Substances 0.000 description 3
230000000069 prophylactic effect Effects 0.000 description 3
108020003175 receptors Proteins 0.000 description 3
102000005962 receptors Human genes 0.000 description 3
230000001105 regulatory effect Effects 0.000 description 3
230000010076 replication Effects 0.000 description 3
108091008146 restriction endonucleases Proteins 0.000 description 3
230000007017 scission Effects 0.000 description 3
238000002741 site-directed mutagenesis Methods 0.000 description 3
239000000243 solution Substances 0.000 description 3
238000001179 sorption measurement Methods 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
238000013268 sustained release Methods 0.000 description 3
239000012730 sustained-release form Substances 0.000 description 3
210000001519 tissue Anatomy 0.000 description 3
229910052722 tritium Inorganic materials 0.000 description 3
241001430294 unidentified retrovirus Species 0.000 description 3
208000007089 vaccinia Diseases 0.000 description 3
UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 2
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
102100038222 60 kDa heat shock protein, mitochondrial Human genes 0.000 description 2
GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
108700023418 Amidases Proteins 0.000 description 2
108091093088 Amplicon Proteins 0.000 description 2
108020005544 Antisense RNA Proteins 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
101000653197 Beet necrotic yellow vein virus (isolate Japan/S) Movement protein TGB3 Proteins 0.000 description 2
101000609456 Beet necrotic yellow vein virus (isolate Japan/S) Protein P26 Proteins 0.000 description 2
FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
108010058432 Chaperonin 60 Proteins 0.000 description 2
ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 2
102100031725 Cortactin-binding protein 2 Human genes 0.000 description 2
241000557626 Corvus corax Species 0.000 description 2
102000028526 Dihydrolipoamide Dehydrogenase Human genes 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
238000012286 ELISA Assay Methods 0.000 description 2
241000672609 Escherichia coli BL21 Species 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
108010015514 Glutamate-tRNA ligase Proteins 0.000 description 2
229920002527 Glycogen Polymers 0.000 description 2
241000282412 Homo Species 0.000 description 2
108090000604 Hydrolases Proteins 0.000 description 2
102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 2
108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 2
108010065805 Interleukin-12 Proteins 0.000 description 2
102000013462 Interleukin-12 Human genes 0.000 description 2
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
108010026217 Malate Dehydrogenase Proteins 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
101100278084 Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) dnaK1 gene Proteins 0.000 description 2
101710116435 Outer membrane protein Proteins 0.000 description 2
108091005804 Peptidases Proteins 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
229920001213 Polysorbate 20 Polymers 0.000 description 2
239000004793 Polystyrene Substances 0.000 description 2
101710197985 Probable protein Rev Proteins 0.000 description 2
102100026126 Proline-tRNA ligase Human genes 0.000 description 2
239000012980 RPMI-1640 medium Substances 0.000 description 2
208000007313 Reproductive Tract Infections Diseases 0.000 description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
102000004191 Ribosomal protein L1 Human genes 0.000 description 2
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
229920002472 Starch Polymers 0.000 description 2
101100117145 Synechocystis sp. (strain PCC 6803 / Kazusa) dnaK2 gene Proteins 0.000 description 2
230000006044 T cell activation Effects 0.000 description 2
230000024932 T cell mediated immunity Effects 0.000 description 2
108091008874 T cell receptors Proteins 0.000 description 2
102000016266 T-Cell Antigen Receptors Human genes 0.000 description 2
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
239000004473 Threonine Substances 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 2
WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 2
229940024545 aluminum hydroxide Drugs 0.000 description 2
102000005922 amidase Human genes 0.000 description 2
125000003277 amino group Chemical group 0.000 description 2
230000001174 ascending effect Effects 0.000 description 2
230000001588 bifunctional effect Effects 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
238000004113 cell culture Methods 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
229960001231 choline Drugs 0.000 description 2
OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 2
101150096566 clpX gene Proteins 0.000 description 2
239000011248 coating agent Substances 0.000 description 2
238000000576 coating method Methods 0.000 description 2
235000017471 coenzyme Q10 Nutrition 0.000 description 2
ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 2
238000012875 competitive assay Methods 0.000 description 2
239000003184 complementary RNA Substances 0.000 description 2
230000001276 controlling effect Effects 0.000 description 2
208000029078 coronary artery disease Diseases 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
230000009260 cross reactivity Effects 0.000 description 2
GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
230000002950 deficient Effects 0.000 description 2
238000011161 development Methods 0.000 description 2
239000000975 dye Substances 0.000 description 2
210000004700 fetal blood Anatomy 0.000 description 2
210000002950 fibroblast Anatomy 0.000 description 2
239000012530 fluid Substances 0.000 description 2
230000006870 function Effects 0.000 description 2
238000001476 gene delivery Methods 0.000 description 2
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
229940096919 glycogen Drugs 0.000 description 2
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
150000004820 halides Chemical class 0.000 description 2
230000007062 hydrolysis Effects 0.000 description 2
238000006460 hydrolysis reaction Methods 0.000 description 2
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
210000000987 immune system Anatomy 0.000 description 2
238000010348 incorporation Methods 0.000 description 2
239000000411 inducer Substances 0.000 description 2
208000000509 infertility Diseases 0.000 description 2
230000036512 infertility Effects 0.000 description 2
231100000535 infertility Toxicity 0.000 description 2
230000002401 inhibitory effect Effects 0.000 description 2
230000000977 initiatory effect Effects 0.000 description 2
238000007912 intraperitoneal administration Methods 0.000 description 2
238000002955 isolation Methods 0.000 description 2
238000002372 labelling Methods 0.000 description 2
239000008101 lactose Substances 0.000 description 2
230000000670 limiting effect Effects 0.000 description 2
GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 2
FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 description 2
238000011068 loading method Methods 0.000 description 2
230000007774 longterm Effects 0.000 description 2
ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 2
239000001095 magnesium carbonate Substances 0.000 description 2
229910000021 magnesium carbonate Inorganic materials 0.000 description 2
235000019359 magnesium stearate Nutrition 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
239000000693 micelle Substances 0.000 description 2
238000002493 microarray Methods 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
229940126619 mouse monoclonal antibody Drugs 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
229910052759 nickel Inorganic materials 0.000 description 2
PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
239000007764 o/w emulsion Substances 0.000 description 2
230000003287 optical effect Effects 0.000 description 2
238000007911 parenteral administration Methods 0.000 description 2
230000001717 pathogenic effect Effects 0.000 description 2
239000012071 phase Substances 0.000 description 2
239000013600 plasmid vector Substances 0.000 description 2
239000004033 plastic Substances 0.000 description 2
229920003023 plastic Polymers 0.000 description 2
101150095517 pmpH gene Proteins 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
229920000136 polysorbate Polymers 0.000 description 2
229920002223 polystyrene Polymers 0.000 description 2
239000004800 polyvinyl chloride Substances 0.000 description 2
229920000915 polyvinyl chloride Polymers 0.000 description 2
230000002062 proliferating effect Effects 0.000 description 2
235000019419 proteases Nutrition 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
101150079601 recA gene Proteins 0.000 description 2
NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 2
238000011160 research Methods 0.000 description 2
238000012552 review Methods 0.000 description 2
102220144047 rs7667001 Human genes 0.000 description 2
CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
150000003384 small molecules Chemical class 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000007790 solid phase Substances 0.000 description 2
239000008107 starch Substances 0.000 description 2
235000019698 starch Nutrition 0.000 description 2
238000010254 subcutaneous injection Methods 0.000 description 2
239000007929 subcutaneous injection Substances 0.000 description 2
125000001424 substituent group Chemical group 0.000 description 2
239000000758 substrate Substances 0.000 description 2
239000000725 suspension Substances 0.000 description 2
230000009885 systemic effect Effects 0.000 description 2
239000000454 talc Substances 0.000 description 2
235000012222 talc Nutrition 0.000 description 2
229910052623 talc Inorganic materials 0.000 description 2
230000008685 targeting Effects 0.000 description 2
238000002560 therapeutic procedure Methods 0.000 description 2
125000003396 thiol group Chemical group [H]S* 0.000 description 2
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
229960001295 tocopherol Drugs 0.000 description 2
229930003799 tocopherol Natural products 0.000 description 2
235000010384 tocopherol Nutrition 0.000 description 2
239000011732 tocopherol Substances 0.000 description 2
238000012546 transfer Methods 0.000 description 2
230000014621 translational initiation Effects 0.000 description 2
229940035936 ubiquinone Drugs 0.000 description 2
210000004291 uterus Anatomy 0.000 description 2
210000001215 vagina Anatomy 0.000 description 2
238000005406 washing Methods 0.000 description 2
GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 2
OCUSNPIJIZCRSZ-ZTZWCFDHSA-N (2s)-2-amino-3-methylbutanoic acid;(2s)-2-amino-4-methylpentanoic acid;(2s,3s)-2-amino-3-methylpentanoic acid Chemical compound CC(C)[C@H](N)C(O)=O.CC[C@H](C)[C@H](N)C(O)=O.CC(C)C[C@H](N)C(O)=O OCUSNPIJIZCRSZ-ZTZWCFDHSA-N 0.000 description 1
125000003088 (fluoren-9-ylmethoxy)carbonyl group Chemical group 0.000 description 1
DHBXNPKRAUYBTH-UHFFFAOYSA-N 1,1-ethanedithiol Chemical compound CC(S)S DHBXNPKRAUYBTH-UHFFFAOYSA-N 0.000 description 1
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 1
UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
238000010600 3H thymidine incorporation assay Methods 0.000 description 1
TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
108010066676 Abrin Proteins 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
102000009027 Albumins Human genes 0.000 description 1
206010002091 Anaesthesia Diseases 0.000 description 1
241000024188 Andala Species 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
206010003445 Ascites Diseases 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
201000001320 Atherosclerosis Diseases 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
238000011725 BALB/c mouse Methods 0.000 description 1
208000004926 Bacterial Vaginosis Diseases 0.000 description 1
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
201000004569 Blindness Diseases 0.000 description 1
101100439426 Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) groEL4 gene Proteins 0.000 description 1
238000011740 C57BL/6 mouse Methods 0.000 description 1
108010029697 CD40 Ligand Proteins 0.000 description 1
101150013553 CD40 gene Proteins 0.000 description 1
102100032937 CD40 ligand Human genes 0.000 description 1
101100221834 Caenorhabditis elegans cpl-1 gene Proteins 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical class [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
108010078791 Carrier Proteins Proteins 0.000 description 1
108010001857 Cell Surface Receptors Proteins 0.000 description 1
102000000844 Cell Surface Receptors Human genes 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
108091029430 CpG site Proteins 0.000 description 1
UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
108010054814 DNA Gyrase Proteins 0.000 description 1
108010041986 DNA Vaccines Proteins 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
229940021995 DNA vaccine Drugs 0.000 description 1
101710088194 Dehydrogenase Proteins 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
108010001625 Diaminopimelate epimerase Proteins 0.000 description 1
208000004145 Endometritis Diseases 0.000 description 1
241000701959 Escherichia virus Lambda Species 0.000 description 1
208000010201 Exanthema Diseases 0.000 description 1
208000001860 Eye Infections Diseases 0.000 description 1
208000026351 Fallopian Tube disease Diseases 0.000 description 1
208000000666 Fowlpox Diseases 0.000 description 1
108700004714 Gelonium multiflorum GEL Proteins 0.000 description 1
108700039691 Genetic Promoter Regions Proteins 0.000 description 1
108010024636 Glutathione Proteins 0.000 description 1
CEXINUGNTZFNRY-BYPYZUCNSA-N Gly-Cys-Gly Chemical group [NH3+]CC(=O)N[C@@H](CS)C(=O)NCC([O-])=O CEXINUGNTZFNRY-BYPYZUCNSA-N 0.000 description 1
241000606768 Haemophilus influenzae Species 0.000 description 1
102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 1
101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 1
101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 1
101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 description 1
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
101150017040 I gene Proteins 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 1
108090000176 Interleukin-13 Proteins 0.000 description 1
108010002386 Interleukin-3 Proteins 0.000 description 1
108010002616 Interleukin-5 Proteins 0.000 description 1
108090001005 Interleukin-6 Proteins 0.000 description 1
108010002586 Interleukin-7 Proteins 0.000 description 1
102000000704 Interleukin-7 Human genes 0.000 description 1
125000000998 L-alanino group Chemical group [H]N([*])[C@](C([H])([H])[H])([H])C(=O)O[H] 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
125000000174 L-prolyl group Chemical group [H]N1C([H])([H])C([H])([H])C([H])([H])[C@@]1([H])C(*)=O 0.000 description 1
125000000773 L-serino group Chemical group [H]OC(=O)[C@@]([H])(N([H])*)C([H])([H])O[H] 0.000 description 1
125000000510 L-tryptophano group Chemical group [H]C1=C([H])C([H])=C2N([H])C([H])=C(C([H])([H])[C@@]([H])(C(O[H])=O)N([H])[*])C2=C1[H] 0.000 description 1
101100521429 Lactococcus lactis subsp. lactis (strain IL1403) prsA gene Proteins 0.000 description 1
241000713666 Lentivirus Species 0.000 description 1
108090001030 Lipoproteins Proteins 0.000 description 1
102000004895 Lipoproteins Human genes 0.000 description 1
DIWRORZWFLOCLC-UHFFFAOYSA-N Lorazepam Chemical compound C12=CC(Cl)=CC=C2NC(=O)C(O)N=C1C1=CC=CC=C1Cl DIWRORZWFLOCLC-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
208000007466 Male Infertility Diseases 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
108010031099 Mannose Receptor Proteins 0.000 description 1
102000018697 Membrane Proteins Human genes 0.000 description 1
BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 1
108060004795 Methyltransferase Proteins 0.000 description 1
102000016397 Methyltransferase Human genes 0.000 description 1
MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
241000187479 Mycobacterium tuberculosis Species 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
KTHDTJVBEPMMGL-VKHMYHEASA-N N-acetyl-L-alanine Chemical compound OC(=O)[C@H](C)NC(C)=O KTHDTJVBEPMMGL-VKHMYHEASA-N 0.000 description 1
KTHDTJVBEPMMGL-UHFFFAOYSA-N N-acetyl-L-alanine Natural products OC(=O)C(C)NC(C)=O KTHDTJVBEPMMGL-UHFFFAOYSA-N 0.000 description 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
108091061960 Naked DNA Proteins 0.000 description 1
108020004711 Nucleic Acid Probes Proteins 0.000 description 1
108010058846 Ovalbumin Proteins 0.000 description 1
238000009004 PCR Kit Methods 0.000 description 1
108090000526 Papain Proteins 0.000 description 1
241001494479 Pecora Species 0.000 description 1
108010013639 Peptidoglycan Proteins 0.000 description 1
102000007456 Peroxiredoxin Human genes 0.000 description 1
201000005702 Pertussis Diseases 0.000 description 1
102000004861 Phosphoric Diester Hydrolases Human genes 0.000 description 1
108090001050 Phosphoric Diester Hydrolases Proteins 0.000 description 1
241000276498 Pollachius virens Species 0.000 description 1
208000003251 Pruritus Diseases 0.000 description 1
101000762949 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Exotoxin A Proteins 0.000 description 1
108010007131 Pulmonary Surfactant-Associated Protein B Proteins 0.000 description 1
102100032617 Pulmonary surfactant-associated protein B Human genes 0.000 description 1
CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
241000700159 Rattus Species 0.000 description 1
241001068263 Replication competent viruses Species 0.000 description 1
206010057190 Respiratory tract infections Diseases 0.000 description 1
101710137010 Retinol-binding protein 3 Proteins 0.000 description 1
102100038247 Retinol-binding protein 3 Human genes 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
108020001027 Ribosomal DNA Proteins 0.000 description 1
108010039491 Ricin Proteins 0.000 description 1
208000007893 Salpingitis Diseases 0.000 description 1
206010070834 Sensitisation Diseases 0.000 description 1
208000019802 Sexually transmitted disease Diseases 0.000 description 1
108010079723 Shiga Toxin Proteins 0.000 description 1
241000193998 Streptococcus pneumoniae Species 0.000 description 1
101710172711 Structural protein Proteins 0.000 description 1
102100027222 T-lymphocyte activation antigen CD80 Human genes 0.000 description 1
206010043376 Tetanus Diseases 0.000 description 1
101100310092 Thauera selenatis serD gene Proteins 0.000 description 1
241000906446 Theraps Species 0.000 description 1
108700009124 Transcription Initiation Site Proteins 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 1
208000025865 Ulcer Diseases 0.000 description 1
241000700618 Vaccinia virus Species 0.000 description 1
208000037009 Vaginitis bacterial Diseases 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical class [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
UZQJVUCHXGYFLQ-AYDHOLPZSA-N [(2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-4-[(2r,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,5-dihydroxy-6-(hy Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2[C@@]1(C=O)C)C)(C)CC(O)[C@]1(CCC(CC14)(C)C)C(=O)O[C@H]1[C@@H]([C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O[C@H]4[C@@H]([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)[C@H](O)[C@@H](CO)O4)O)[C@H](O)[C@@H](CO)O3)O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UZQJVUCHXGYFLQ-AYDHOLPZSA-N 0.000 description 1
FHICGHSMIPIAPL-HDYAAECPSA-N [2-[3-[6-[3-[(5R,6aS,6bR,12aR)-10-[6-[2-[2-[4,5-dihydroxy-3-(3,4,5-trihydroxyoxan-2-yl)oxyoxan-2-yl]ethoxy]ethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-5-hydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carbonyl]peroxypropyl]-5-[[5-[8-[3,5-dihydroxy-4-(3,4,5-trihydroxyoxan-2-yl)oxyoxan-2-yl]octoxy]-3,4-dihydroxy-6-methyloxan-2-yl]methoxy]-3,4-dihydroxyoxan-2-yl]propoxymethyl]-5-hydroxy-3-[(6S)-6-hydroxy-2,6-dimethylocta-2,7-dienoyl]oxy-6-methyloxan-4-yl] (2E,6S)-6-hydroxy-2-(hydroxymethyl)-6-methylocta-2,7-dienoate Chemical compound C=C[C@@](C)(O)CCC=C(C)C(=O)OC1C(OC(=O)C(\CO)=C\CC[C@](C)(O)C=C)C(O)C(C)OC1COCCCC1C(O)C(O)C(OCC2C(C(O)C(OCCCCCCCCC3C(C(OC4C(C(O)C(O)CO4)O)C(O)CO3)O)C(C)O2)O)C(CCCOOC(=O)C23C(CC(C)(C)CC2)C=2[C@@]([C@]4(C)CCC5C(C)(C)C(OC6C(C(O)C(O)C(CCOCCC7C(C(O)C(O)CO7)OC7C(C(O)C(O)CO7)O)O6)O)CC[C@]5(C)C4CC=2)(C)C[C@H]3O)O1 FHICGHSMIPIAPL-HDYAAECPSA-N 0.000 description 1
230000002159 abnormal effect Effects 0.000 description 1
238000005903 acid hydrolysis reaction Methods 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
125000003172 aldehyde group Chemical group 0.000 description 1
125000000217 alkyl group Chemical group 0.000 description 1
230000000735 allogeneic effect Effects 0.000 description 1
229940037003 alum Drugs 0.000 description 1
ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
229940024546 aluminum hydroxide gel Drugs 0.000 description 1
SMYKVLBUSSNXMV-UHFFFAOYSA-K aluminum;trihydroxide;hydrate Chemical compound O.[OH-].[OH-].[OH-].[Al+3] SMYKVLBUSSNXMV-UHFFFAOYSA-K 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
229960003896 aminopterin Drugs 0.000 description 1
238000001949 anaesthesia Methods 0.000 description 1
230000037005 anaesthesia Effects 0.000 description 1
150000008064 anhydrides Chemical class 0.000 description 1
230000008350 antigen-specific antibody response Effects 0.000 description 1
239000003963 antioxidant agent Substances 0.000 description 1
235000006708 antioxidants Nutrition 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
239000000823 artificial membrane Substances 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
238000002820 assay format Methods 0.000 description 1
125000004429 atom Chemical group 0.000 description 1
244000052616 bacterial pathogen Species 0.000 description 1
WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
238000001574 biopsy Methods 0.000 description 1
239000002981 blocking agent Substances 0.000 description 1
238000009835 boiling Methods 0.000 description 1
210000000988 bone and bone Anatomy 0.000 description 1
210000001185 bone marrow Anatomy 0.000 description 1
239000007975 buffered saline Substances 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000002775 capsule Substances 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
238000012832 cell culture technique Methods 0.000 description 1
230000007910 cell fusion Effects 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
239000013522 chelant Substances 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
210000000991 chicken egg Anatomy 0.000 description 1
208000028512 chlamydia infectious disease Diseases 0.000 description 1
235000012000 cholesterol Nutrition 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
101150043719 clpP1 gene Proteins 0.000 description 1
238000001246 colloidal dispersion Methods 0.000 description 1
239000000084 colloidal system Substances 0.000 description 1
230000009918 complex formation Effects 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
230000001268 conjugating effect Effects 0.000 description 1
230000021615 conjugation Effects 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000000139 costimulatory effect Effects 0.000 description 1
239000003431 cross linking reagent Substances 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
230000001086 cytosolic effect Effects 0.000 description 1
230000003013 cytotoxicity Effects 0.000 description 1
231100000135 cytotoxicity Toxicity 0.000 description 1
230000006378 damage Effects 0.000 description 1
210000001787 dendrite Anatomy 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
239000003599 detergent Substances 0.000 description 1
239000003937 drug carrier Substances 0.000 description 1
238000004520 electroporation Methods 0.000 description 1
238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
239000000839 emulsion Substances 0.000 description 1
238000005538 encapsulation Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
201000005884 exanthem Diseases 0.000 description 1
210000001808 exosome Anatomy 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000835 fiber Substances 0.000 description 1
239000011152 fibreglass Substances 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
238000001415 gene therapy Methods 0.000 description 1
102000054766 genetic haplotypes Human genes 0.000 description 1
239000011521 glass Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
229960003180 glutathione Drugs 0.000 description 1
101150077981 groEL gene Proteins 0.000 description 1
230000005802 health problem Effects 0.000 description 1
208000006454 hepatitis Diseases 0.000 description 1
231100000283 hepatitis Toxicity 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
230000008348 humoral response Effects 0.000 description 1
210000004754 hybrid cell Anatomy 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
230000036737 immune function Effects 0.000 description 1
230000006872 improvement Effects 0.000 description 1
201000001371 inclusion conjunctivitis Diseases 0.000 description 1
210000004969 inflammatory cell Anatomy 0.000 description 1
208000037798 influenza B Diseases 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
238000007917 intracranial administration Methods 0.000 description 1
238000007852 inverse PCR Methods 0.000 description 1
239000003456 ion exchange resin Substances 0.000 description 1
229920003303 ion-exchange polymer Polymers 0.000 description 1
229910052742 iron Inorganic materials 0.000 description 1
230000002427 irreversible effect Effects 0.000 description 1
108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
230000021633 leukocyte mediated immunity Effects 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
210000001165 lymph node Anatomy 0.000 description 1
239000006166 lysate Substances 0.000 description 1
230000002934 lysing effect Effects 0.000 description 1
230000002101 lytic effect Effects 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
239000006249 magnetic particle Substances 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
239000003550 marker Substances 0.000 description 1
239000000463 material Substances 0.000 description 1
230000035800 maturation Effects 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
229910044991 metal oxide Inorganic materials 0.000 description 1
150000004706 metal oxides Chemical class 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
239000002480 mineral oil Substances 0.000 description 1
235000010446 mineral oil Nutrition 0.000 description 1
238000010172 mouse model Methods 0.000 description 1
230000035772 mutation Effects 0.000 description 1
230000000869 mutational effect Effects 0.000 description 1
239000002088 nanocapsule Substances 0.000 description 1
210000000822 natural killer cell Anatomy 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
239000002853 nucleic acid probe Substances 0.000 description 1
230000000269 nucleophilic effect Effects 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
229940092253 ovalbumin Drugs 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
229940055729 papain Drugs 0.000 description 1
235000019834 papain Nutrition 0.000 description 1
230000008506 pathogenesis Effects 0.000 description 1
239000008188 pellet Substances 0.000 description 1
210000003200 peritoneal cavity Anatomy 0.000 description 1
210000004303 peritoneum Anatomy 0.000 description 1
239000000546 pharmaceutical excipient Substances 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
150000004633 phorbol derivatives Chemical class 0.000 description 1
239000002644 phorbol ester Substances 0.000 description 1
150000008300 phosphoramidites Chemical class 0.000 description 1
101150010607 pmpA gene Proteins 0.000 description 1
101150116202 pmpF gene Proteins 0.000 description 1
108700028325 pokeweed antiviral Proteins 0.000 description 1
229920000747 poly(lactic acid) Polymers 0.000 description 1
229920002627 poly(phosphazenes) Polymers 0.000 description 1
239000013641 positive control Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
239000003755 preservative agent Substances 0.000 description 1
230000002265 prevention Effects 0.000 description 1
210000004986 primary T-cell Anatomy 0.000 description 1
238000012545 processing Methods 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
239000001397 quillaja saponaria molina bark Substances 0.000 description 1
206010037844 rash Diseases 0.000 description 1
230000000601 reactogenic effect Effects 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
229910052702 rhenium Inorganic materials 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
229930182490 saponin Natural products 0.000 description 1
150000007949 saponins Chemical group 0.000 description 1
238000003345 scintillation counting Methods 0.000 description 1
239000006152 selective media Substances 0.000 description 1
230000008313 sensitization Effects 0.000 description 1
238000002864 sequence alignment Methods 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
230000000405 serological effect Effects 0.000 description 1
230000035939 shock Effects 0.000 description 1
230000011664 signaling Effects 0.000 description 1
210000003491 skin Anatomy 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
239000011343 solid material Substances 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
125000006850 spacer group Chemical group 0.000 description 1
238000011895 specific detection Methods 0.000 description 1
238000004611 spectroscopical analysis Methods 0.000 description 1
210000000952 spleen Anatomy 0.000 description 1
238000012289 standard assay Methods 0.000 description 1
229940031000 streptococcus pneumoniae Drugs 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
125000004434 sulfur atom Chemical group 0.000 description 1
208000024891 symptom Diseases 0.000 description 1
WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
HNKJADCVZUBCPG-UHFFFAOYSA-N thioanisole Chemical compound CSC1=CC=CC=C1 HNKJADCVZUBCPG-UHFFFAOYSA-N 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
206010044325 trachoma Diseases 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
230000009261 transgenic effect Effects 0.000 description 1
238000003146 transient transfection Methods 0.000 description 1
238000011269 treatment regimen Methods 0.000 description 1
201000008827 tuberculosis Diseases 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
150000003668 tyrosines Chemical class 0.000 description 1
231100000397 ulcer Toxicity 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
229940045145 uridine Drugs 0.000 description 1
238000002255 vaccination Methods 0.000 description 1
239000013603 viral vector Substances 0.000 description 1
238000001262 western blot Methods 0.000 description 1
QAOHCFGKCWTBGC-QHOAOGIMSA-N wybutosine Chemical compound C1=NC=2C(=O)N3C(CC[C@H](NC(=O)OC)C(=O)OC)=C(C)N=C3N(C)C=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O QAOHCFGKCWTBGC-QHOAOGIMSA-N 0.000 description 1
QAOHCFGKCWTBGC-UHFFFAOYSA-N wybutosine Natural products C1=NC=2C(=O)N3C(CCC(NC(=O)OC)C(=O)OC)=C(C)N=C3N(C)C=2N1C1OC(CO)C(O)C1O QAOHCFGKCWTBGC-UHFFFAOYSA-N 0.000 description 1
239000011701 zinc Chemical class 0.000 description 1
229910052725 zinc Chemical class 0.000 description 1