Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
  • A01P3/00—Fungicides

Definitions

• the present invention relates to a composition and method for inhibiting the growth of plant pathogenic fungi. More specifically, the present invention relates to a composition for inhibiting the growth of plant pathogenic fungi that contains mercaptopropionic acid as an active ingredient. The present invention also relates to a method for inhibiting the growth of plant pathogenic fungi that utilizes mercaptopropionic acid.
• Aerial spraying is an effective method of controlling above-ground diseases, but due to concerns about the environmental impact of spraying, only a limited number of pesticides can be used, and it is desirable to use appropriate pesticides at the appropriate time. Furthermore, there are only a limited number of cases where pesticides that are effective in controlling soil diseases are also used to control above-ground diseases.
• mercaptopropionic acid has been previously disclosed for use in combination with bismuth as a disinfectant for biomedical uses, including the treatment of bacterial biofilms and other uses (Patent Document 1).
• this technology is a medical technology and is not related to the effects of plant protection.
• mercaptopropionic acid is merely listed as an example of a thiol compound, and its specific effects are not shown.
• the present invention aims to provide a composition or method that is useful for suppressing the growth of plant pathogens.
• the present invention is preferably carried out in the manner described below, but is not limited thereto.
• Embodiment 1 A composition for inhibiting the growth of plant pathogens, comprising mercaptopropionic acid.
• aspect 2 The composition according to aspect 1, wherein the plant pathogen is a plant pathogen that causes a soil-borne disease or an above-ground disease.
• Embodiment 3 A method for inhibiting the growth of plant pathogens, comprising a step of contacting mercaptopropionic acid with plant pathogens.
• Embodiment 4 The method according to embodiment 3, wherein the plant pathogen is a plant pathogen that causes a soil-borne disease or an above-ground disease.
• the present invention By utilizing the present invention, it is possible to provide a composition or method that is useful for inhibiting the growth of plant pathogens. Furthermore, by using the technology of the present invention, it is possible to protect plants from disease. Since the technology of the present invention is effective against plant pathogens that cause soil-borne diseases, it is possible to effectively protect plants from soil-borne diseases by using the composition or method of the present invention. Furthermore, since the technology of the present invention is effective against plant pathogens that cause above-ground diseases, it is possible to effectively protect plants from above-ground diseases by using the composition or method of the present invention.
• FIG. 1 shows the results of investigating the antibacterial activity (growth inhibitory activity) of mercaptopropionic acid against bacteria of the genus Pythium.
• FIG. 2 is a diagram showing the distance from a filter paper impregnated with mercaptopropionic acid to the tip of a hypha of a Pythium fungus.
• FIG. 3 is a graph showing the effect of volatility of mercaptopropionic acid in an antibacterial test.
• FIG. 4 shows the results of investigating the antibacterial activity (growth inhibitory activity) of mercaptopropionic acid against fungi of the genus Fusarium.
• FIG. 5 shows the results of investigating the antibacterial activity (growth inhibitory activity) of mercaptopropionic acid against bacteria of the genus Ralstonia.
• FIG. 6 shows the results of investigating the effective concentration of mercaptopropionic acid against bacteria of the genus Pythium.
• FIG. 7 shows the results of investigating the effective concentration of mercaptopropionic acid against Ralstonia bacteria.
• FIG. 8 is a graph showing the change in the number of surviving cucumbers over time.
• FIG. 9 is a graph showing the growth state of cucumber seedlings as a result of investigating the effect of mercaptopropionic acid in protecting cucumber from diseases.
• FIG. 10 is a diagram showing the incidence rate of bacterial wilt, a soil-borne disease, in tomato leaves.
• FIG. 11 is a diagram showing the growth state of tomatoes two days after inoculation with Ralstonia bacteria, as a result of investigating the effect of mercaptopropionic acid in protecting tomatoes from disease.
• FIG. 12 is a graph showing the growth state of cucumbers 7 days after inoculation with anthracnose, a disease of above-ground parts, as a result of investigating the effect of mercaptopropionic acid in protecting above-ground parts of cucumbers from disease.
• FIG. 13 is a diagram showing the growth state of cucumbers 11 days after inoculation with anthracnose, a disease of above-ground parts, as a result of investigating the effect of mercaptopropionic acid in protecting above-ground parts of cucumbers from disease.
• composition for Inhibiting the Growth of Plant Pathogens One aspect of the present invention is a composition for inhibiting the growth of plant pathogens, which contains mercaptopropionic acid.
• Mercaptopropionic acid can be contained as an active ingredient in the composition of the present invention.
• the mercaptopropionic acid used in the present invention is preferably 3-mercaptopropionic acid.
• 3-mercaptopropionic acid is a compound represented by the molecular formula C 3 H 6 O 2 S, and its CAS registry number is 107-96-0.
• 3-mercaptopropionic acid is known to be used as a metal masking agent or reducing agent.
• the amount of mercaptopropionic acid in the composition of the present invention is not particularly limited, and may be at a level that can inhibit the growth of plant pathogens.
• the amount can be appropriately set depending on the type of plant pathogen to be targeted.
• the content of mercaptopropionic acid in the composition of the present invention is, as a concentration at the time of use (final concentration), for example, 1 ⁇ M or more, preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, even more preferably 1 mM or more, 2 mM or more, or 5 mM or more.
• the content of mercaptopropionic acid in the composition of the present invention is, as a concentration at the time of use, for example, 10 M or less, preferably 1 M or less, more preferably 100 mM or less, even more preferably 10 mM or less.
• the content of mercaptopropionic acid in the composition of the present invention is, as a concentration at the time of use, typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, even more preferably 1 mM to 10 mM.
• the content of mercaptopropionic acid in the composition of the present invention can be measured using HPLC, etc.
• the composition of the present invention when the composition of the present invention is a liquid, it can be in the form of a concentrated solution, and the concentration ratio is, for example, 10 times, 20 times, 50 times, 100 times, 200 times, 500 times, 1000 times, 2000 times, 5000 times, or 10000 times the concentration at the time of use.
• the concentration ratio can be, for example, 10 to 10000 times, preferably 50 to 10000 times, more preferably 100 to 10000 times, and even more preferably 1000 to 10000 times the concentration at the time of use.
• the composition of the present invention when the composition of the present invention is in the form of a concentrated solution, it can be appropriately diluted with a liquid such as water before use.
• the composition of the present invention may also be a solid formulation.
• the content of mercaptopropionic acid in the composition can be adjusted to, for example, 100 times, 200 times, 500 times, 1000 times, 10000 times, or 100000 times the concentration at the time of use.
• the content of mercaptopropionic acid in the composition can be, for example, 100 to 100000 times, preferably 200 to 100000 times, more preferably 500 to 100000 times, and even more preferably 1000 to 100000 times the concentration at the time of use.
• the composition of the present invention is in the form of a solid formulation, it can be used after being appropriately dissolved or dispersed in a liquid such as water.
• the amount of mercaptopropionic acid used in the composition of the present invention is not particularly limited, and can be appropriately set according to the form of use, the type of plant pathogenic fungus to be targeted, etc.
• the concentration (amount used) of mercaptopropionic acid is not particularly limited, but can be adjusted to 1 ⁇ M or more , preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, and even more preferably 1 mM or more, 2 mM or more, or 5 mM or more per 1 cm3 of soil.
• the upper limit is not particularly limited, but the concentration (amount used) of mercaptopropionic acid can be adjusted to 10 M or less, preferably 1 M or less, more preferably 100 mM or less, and even more preferably 10 mM or less per 1 cm3 of soil.
• the concentration (amount used) of mercaptopropionic acid is typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, and even more preferably 1 mM to 10 mM.
• the concentration (amount used) of mercaptopropionic acid is not particularly limited, but can be adjusted to 1 ⁇ M or more, preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, and even more preferably 1 mM or more, 2 mM or more, or 5 mM or more per plant.
• the upper limit is not particularly limited, but the concentration (amount used) of mercaptopropionic acid can be adjusted to 10 M or less, preferably 1 M or less, more preferably 100 mM or less, and even more preferably 10 mM or less per plant.
• the concentration (amount used) of mercaptopropionic acid is typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, and even more preferably 1 mM to 10 mM.
• the mercaptopropionic acid used in the present invention may be chemically synthesized or may be obtained from a natural product.
• the mercaptopropionic acid may be synthesized or purified by a method known to those skilled in the art, or may be a commercially available product. In the present invention, commercially available mercaptopropionic acid is preferably used.
• Mercaptopropionic acid may also be a product of a microorganism. That is, the mercaptopropionic acid used in the present invention may be derived from a microorganism. Examples of products of microorganisms include metabolic products, which may be primary or secondary metabolic products. Microorganisms that produce mercaptopropionic acid include bacteria, specifically bacteria of the genus Pseudomonas. Pseudomonas bacteria belong to the family Pseudomonadaceae in the class Gammaproteobacteria of the phylum Proteobacteria, and are characterized as gram-negative aerobic rods. Specific examples of Pseudomonas bacteria include Pseudomonas protegens and Pseudomonas aeruginosa, but are not limited to these.
• the composition of the present invention is applied to plant pathogens.
• plant pathogens are not limited to bacteria and fungi, but refer to microorganisms that cause plant diseases (also referred to as “plant pathogenic microorganisms").
• the plant pathogens may be either bacteria or fungi.
• the plant pathogens to which the composition of the present invention is applied are not particularly limited, but are preferably plant pathogens that cause soil-borne diseases or above-ground diseases (diseases of the above-ground parts of plants).
• above-ground diseases refer to diseases that occur in parts of the plant body that are above ground (including parts that are not in contact with the ground).
• Soil-borne diseases are often caused by pathogenic microorganisms that live in the soil.
• pathogenic microorganisms that live in the soil include bacteria and fungi, and examples of fungi include filamentous fungi (including oomycetes).
• filamentous fungi that are pathogenic microorganisms include fungi of the genus Pythium (Pythium ultimum, Pythium aphanidermatum, Pythium megalacanthum, etc.), fungi of the genus Fusarium (Fusarium oxysporum, etc.), and fungi of the genus Fusarium (Fusarium oxysporum, etc.).
• fungi examples include Fusarium oxysporum, Fusarium graminearum, Fusarium solani, etc.), Rhizoctonia (Rhizoctonia solani, etc.), and Thielaviopsis.
• pathogenic microorganisms other than filamentous fungi include bacteria of the genus Erwinia (Erwinia carotovora, etc.), bacteria of the genus Ralstonia (Ralstonia solanacearum, etc.), bacteria of the genus Pectobacterium (Pectobacterium kaempferi, etc.), and bacteria of the genus Bacteria.
• examples include bacteria of the genus Burkholderia (such as Burkholderia glumae), and bacteria of the genus Agrobacterium (such as Agrobacterium tumefaciens).
• soil-borne diseases include seedling damping-off, Pythium rot, bacterial wilt, seedling root rot, wilt, leaf rot, root rot, vine splitting, damping-off, yellowing, bottom rot, root rot weakening, cone browning, rot, leaf blight, dry rot, stem blight, red mold, bulb rot, stem rot wilt, stem rot, root rot wilt, black streaked fruit rot, semi-blight, black spot, Panama disease, brown rot, Fusarium wilt, stem rot, crest blight
• bud blight spider blight, brown sheath blight, bottom blight, Rhizoctonia disease, corm rot, black bruise, hole leaf blight, dry root rot, large-grain white blight, tiger spot, bottom rot, fruit rot, back break, forest root rot, butt rot, pod rot,
• Pathogenic microorganisms that cause above-ground plant diseases include, for example, bacteria and fungi.
• Fungi include, for example, the genus Colletotrichum (Colletotrichum orbiculare, Colletotrichum gloeosporioides, Colletotrichum acutatum, etc.), Botrytis ) genus (Botrytis cinerea, etc.), Sphaerotheca genus (Sphaerotheca fuliginea, etc.), Peronospora genus (Peronospora parasitica, etc.), Pseudoperonospora ) genus (Pseudoperonospora cubensis, etc.), Pythium genus (Pythium ultimum, Pythium aphanidermatum, Pythium megalacanthum, etc.), Fusarium genus Examples include fungi (Fusarium oxysporum, Fusarium graminearum,
• pathogenic bacteria that cause above-ground plant diseases include bacteria of the genus Pseudomonas (e.g., Pseudomonas syringae), bacteria of the genus Xanthomonas (e.g., Xanthomonas campestris), bacteria of the genus Erwinia (Erwinia amylovora, Erwinia carotovora), bacteria of the genus Clavibacter (Clavibacter michiganensis), and bacteria of the genus Clavibacter (Clavibacter michiganensis).
• Pseudomonas e.g., Pseudomonas syringae
• bacteria of the genus Xanthomonas e.g., Xanthomonas campestris
• bacteria of the genus Erwinia Erwinia amylovora, Erwinia carotovora
• bacteria of the genus Clavibacter C
• ichiganensis etc.
• Ralstonia bacteria Ralstonia solanacearum, etc.
• Pectobacterium bacteria Pectobacterium carotovorum, etc.
• Burkholderia bacteria Burkholderia glumae, etc.
• Agrobacterium bacteria Agrobacterium tumefaciens, etc.
• Diseases of above-ground plants include, for example, anthracnose, gray mold, leaf mold, downy mildew, bacterial spot, leaf spot, wildfire disease, bacterial hole, black rot, fire blight, wilt, leaf rot, vine splitting, damping off, yellowing, bottom rot, rot, leaf blight, dry rot, stem blight, red mold, stem rot wilt, stem rot, black streaked fruit rot, semi-blight, black spot, Panama disease, brown rot, and foliage.
• sarium disease stock rot, sheath blight, bud blight, spider blight, brown sheath blight, bottom rot, Rhizoctonia disease, corm rot, black spot disease, hole leaf blight, large-grain white blight, tiger spot disease, bottom rot, fruit rot, back break disease, forest root rot, butt rot, pod rot, skin rot, brown spot disease, brown spot disease, white leaf rot, Rhizoctonia leaf sheath rot, etc.
• the plants targeted in the present invention are not particularly limited, but are preferably agricultural crops.
• Types of agricultural crops include, but are not particularly limited to, vegetables, grains, fruits, flowers, and beans. Specific examples include melons (cucumber, watermelon, pumpkin, zucchini, gourd, loofah, wax gourd, trumpet gourd, bottle gourd, bitter melon (bitter melon, bitter gourd), melon, etc.), tubers (potato, sweet potato, taro, Chinese yam, Chinese yam, etc.), root vegetables (turnip, radish, radish, wasabi, horseradish, burdock, Chinese chive, ginger, carrot, scallion, lotus root, lily root, etc.), leafy vegetables (cabbage, radish, Chinese chive ...
• the composition of the present invention may contain additives such as excipients, thickeners, binders, stabilizers, preservatives, pH adjusters, colorants, and flavoring agents in addition to mercaptopropionic acid.
• additives such as excipients, thickeners, binders, stabilizers, preservatives, pH adjusters, colorants, and flavoring agents in addition to mercaptopropionic acid.
• the various additives are not particularly limited, but materials known in the agricultural chemicals technical field can be used, and the amount of the additives can be appropriately adjusted based on the known techniques of those skilled in the art.
• the form of the composition of the present invention may be any of liquid, solid, gel, paste, etc., and can be appropriately set depending on the usage situation, etc. When the composition of the present invention is a liquid, it can also be used as a spray-type liquid agent.
• composition of the present invention can be used as an agricultural chemical, although there is no particular limitation thereto. Therefore, the composition of the present invention may be an agricultural chemical composition.
• the composition of the present invention may also be referred to as an agent, and the composition for inhibiting the growth of plant pathogenic fungi of the present invention may be referred to as a plant pathogenic fungi growth inhibitor.
• composition of the present invention is characterized by having an effect of inhibiting the growth of plant pathogens, and based on this effect, it is possible to prevent plants from disease and protect plant growth. Therefore, the composition of the present invention can be used for the purpose of preventing plant disease or for plant protection (more specifically, for protecting plant growth).
• the plant disease is preferably a soil-borne disease.
• One embodiment of the present invention is a method for inhibiting the growth of plant pathogenic fungi, comprising the step of contacting mercaptopropionic acid with plant pathogenic fungi.
• the mercaptopropionic acid used in the method of the present invention is as described above.
• the plant pathogen and the plant caused by the pathogen are also as described above.
• the method of the present invention is characterized in that mercaptopropionic acid is brought into contact with plant pathogens.
• the contact of mercaptopropionic acid with plant pathogens is not particularly limited, but can be achieved, for example, by applying mercaptopropionic acid to soil or plants.
• soil means soil in which plants can grow.
• the soil used may be, for example, culture soil, culture soil for seedlings, or culture soil for seedlings. In addition, soil from mountains and fields that has not been treated in any way may be used as is.
• the particle size of the soil is also not particularly limited, and any soil may be used as long as plants can grow therein.
• the soil to which mercaptopropionic acid is applied is preferably soil in the vicinity of the plant (for example, soil within a range of 10 cm from the plant).
• the plant to which mercaptopropionic acid is added may be a plant that is affected by the plant pathogenic fungus of the present invention, and is preferably an agricultural crop.
• the types and specific examples of the agricultural crop are as described above.
• the state of the plant to which mercaptopropionic acid is applied is not particularly limited, and may be a seed or a seedling, or a plant that has already grown.
• the seed may be a rooted seed.
• mercaptopropionic acid can be applied to any part of the plant, such as the roots, leaves, stems, branches, trunks, flowers, or fruits.
• the disease caused by the plant pathogen is a soil-borne disease
• the state of the plant to which mercaptopropionic acid is applied is preferably a seed or a seedling, and particularly preferably a seed.
• the onset of soil-borne disease can be suppressed, and soil-borne disease in plants can be more effectively controlled.
• the state of the plant to which mercaptopropionic acid is applied is preferably a seedling or a plant that has already grown, and particularly preferably a seedling.
• the method and means for the application are not particularly limited as long as the effects of the present invention can be obtained.
• the application operation can be carried out by contacting mercaptopropionic acid itself, or a composition containing mercaptopropionic acid, with soil or plants. Both mercaptopropionic acid and compositions containing it may be suspended or dissolved in water (or an aqueous solution), and the application operation may be carried out by contacting the resulting solution with soil or plants.
• the composition containing mercaptopropionic acid may be in the form of a liquid or a solid.
• the mercaptopropionic acid can be applied to the soil or the plant by spraying, dripping, immersion, or other operations.
• a separate container may be prepared and the perforated container containing the soil or the plant may be placed in the container containing the liquid to carry out the operation.
• the acid may be applied by placing the solid on or in the soil, or by contacting the solid with the surface of the plant body.
• mercaptopropionic acid is a volatile substance, it can be brought into contact with plant pathogens in the form of a gas component (in a gaseous state). When mercaptopropionic acid is applied to soil or a plant, it can volatilize from there and come into contact with plant pathogens in the form of a gas component. Mercaptopropionic acid, which has volatile properties, can be brought into contact with plant pathogens even if it is applied at a position away from the plant pathogens, unlike other non-volatile substances. Furthermore, if it is in the form of a gas component, it can be brought into contact with plant pathogens over a wide area, and in some cases, it can be brought into contact with plant pathogens quickly.
• mercaptopropionic acid when it is used in the form of a gas component, for example, mercaptopropionic acid can be applied under conditions where the soil is covered with vinyl or the like.
• mercaptopropionic acid when mercaptopropionic acid is used in the form of a gaseous component, mercaptopropionic acid itself or a composition containing it can be used as a fumigant, although there are no particular limitations thereon.
• the concentration (final concentration) of mercaptopropionic acid when used is not particularly limited, but is, for example, 1 ⁇ M or more, preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, and even more preferably 1 mM or more, 2 mM or more, or 5 mM or more.
• the upper limit of the concentration (final concentration) of mercaptopropionic acid when used is, for example, 10 M or less, preferably 1 M or less, more preferably 100 mM or less, and even more preferably 10 mM or less.
• the concentration (final concentration) of mercaptopropionic acid when used is typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, and even more preferably 1 mM to 10 mM.
• the amount of mercaptopropionic acid applied is not particularly limited and can be appropriately set according to the application form, the type of target plant, etc.
• mercaptopropionic acid when mercaptopropionic acid is applied to soil, it can be applied in an amount of 1 ⁇ M or more , preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, and even more preferably 1 mM or more, 2 mM or more, or 5 mM or more per 1 cm 3 of soil.
• the upper limit is not particularly limited, but it can be 10 M or less, preferably 1 M or less, more preferably 100 mM or less, and even more preferably 10 mM or less per 1 cm 3 of soil.
• the concentration (amount) of mercaptopropionic acid used is typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, and even more preferably 1 mM to 10 mM.
• mercaptopropionic acid When mercaptopropionic acid is applied to a plant, it can be applied in an amount of 1 ⁇ M or more, preferably 10 ⁇ M or more, more preferably 0.1 mM or more, 0.2 mM or more, or 0.5 mM or more, and even more preferably 1 mM or more, 2 mM or more, or 5 mM or more per plant.
• the upper limit is not particularly limited, but it can be 10 M or less, preferably 1 M or less, more preferably 100 mM or less, and even more preferably 10 mM or less per plant.
• the concentration (amount) of mercaptopropionic acid used is typically, for example, 1 ⁇ M to 10 M, preferably 10 ⁇ M to 1 M, more preferably 0.1 mM to 100 mM, and even more preferably 1 mM to 10 mM.
• the amount of mercaptopropionic acid applied can be adjusted by the concentration in a formulation containing it and the amount of the formulation applied.
• the time of application may be any of the following: before sowing the plants, at the time of sowing, or after sowing (including the seedling stage, planting stage, etc.), and is not particularly limited.
• the application of mercaptopropionic acid is preferably performed within 3 days before or after sowing the plants.
• "sowing” means planting a plant in soil as the starting point for growth, and includes, for example, sowing seeds in soil.
• the specific timing is not particularly limited, but for example, within 7 days before sowing, within 6 days before sowing, within 5 days before sowing, within 4 days before sowing, within 3 days before sowing, within 2 days before sowing, within 1 day before sowing, within 12 hours before sowing, within 6 hours before sowing, within 3 hours before sowing, within 1 hour before sowing, within 30 minutes before sowing, within 10 minutes before sowing, within 5 minutes before sowing, within 1 minute before sowing, or within 30 seconds before sowing.
• the application time may be within 7 days, 6 days, 5 days, or 4 days after sowing, or may be within 2 days, 1 day, 12 hours, 6 hours, 3 hours, 1 hour, 30 minutes, 10 minutes, 5 minutes, 1 minute, or 30 seconds after sowing.
• the disease caused by the plant pathogen is a soil-borne disease
• the mercaptopropionic acid contacts the plant pathogen at an early stage of plant growth, so from this perspective, it is preferable that the period between the application time and the plant sowing time is short, and it is preferable to apply the mercaptopropionic acid within 3 days before or after sowing, or within 1 day before or after sowing, for example.
• mercaptopropionic acid may be applied either before or after sowing the plant.
• the specific time is not particularly limited, but may be, for example, within 7 days before sowing, within 6 days before sowing, within 5 days before sowing, within 4 days before sowing, within 3 days before sowing, within 2 days before sowing, within 1 day before sowing, within 12 hours before sowing, within 6 hours before sowing, within 3 hours before sowing, within 1 hour before sowing, within 30 minutes before sowing, within 10 minutes before sowing, within 5 minutes before sowing, within 1 minute before sowing, or within 30 seconds before sowing.
• mercaptopropionic acid When mercaptopropionic acid is applied after sowing of the plant, it may be applied several weeks (e.g., 2 to 6 weeks) to several months (e.g., 2 to 6 months) after sowing, or it may be applied several years (e.g., 2 to 6 years) after sowing.
• mercaptopropionic acid when mercaptopropionic acid is applied to the leaves of the plant, it is preferable to apply it after confirming that the leaves of the plant have unfolded. The same applies to the flowers and fruits of the plant, and it is preferable to apply mercaptopropionic acid after confirming the presence of the flowers and fruits, respectively.
• Mercaptopropionic acid may be continuously contacted with the plant pathogenic fungus multiple times. Continuous contact with mercaptopropionic acid can effectively sustain the growth inhibition of the plant pathogenic fungus. Further contact with mercaptopropionic acid may be performed one or more times, and may be two or more times, three or more times, four or more times, or five or more times.
• the interval between further contacts with mercaptopropionic acid is not particularly limited, but may be, for example, 6 hours or more, 12 hours or more, 1 day or more, 2 days or more, 3 days or more, or 4 days or more, and may be 10 days or less, 7 days or less, 6 days or less, 5 days or less, 4 days or less, 3 days or less, 2 days or less, 1 day or less, or 12 hours or less.
• Further contact with mercaptopropionic acid can be performed through further application of mercaptopropionic acid to the soil or plant, and the above number of times and timings can be applied to further application of mercaptopropionic acid to the soil or plant.
• the form or means of applying mercaptopropionic acid is not particularly limited, and can be the same as described above.
• the amount of mercaptopropionic acid further applied is also not particularly limited, and can be the same as described above.
• the method of the present invention is characterized by suppressing the growth of plant pathogens, and by using the method, it is possible to prevent plants from disease or protect the growth of plants. Therefore, another aspect of the present invention is a method for preventing plants from disease or a method for protecting plants (more specifically, a method for protecting the growth of plants).
• the plant disease is preferably a soil-borne disease or an above-ground disease.
• Both the method for preventing plants from disease and the method for protecting plants include a step of contacting mercaptopropionic acid with plant pathogens, and these methods may also include a step of carrying out the above-mentioned method (i.e., a method for suppressing the growth of plant pathogens).
• 3-mercaptopropionic acid (Tokyo Chemical Industry Co., Ltd.) was used as mercaptopropionic acid (MPA).
• MPA mercaptopropionic acid
• MAFF425494 a known antibacterial substance, 2,4-diacetylphloroglucinol (DAPG) was used as a control. The antibacterial test was performed using a paired culture method.
• a PDA solid medium (3.9 g PDA (Sigma-Aldrich), 0.3 g Agar (Wako) per 100 mL) was prepared in a petri dish (diameter 60 mm), and a bacterial colony of Pythium bacteria (diameter approximately 6 mm) was placed on one side of the dish at a distance of approximately 20 mm, and a filter paper soaked in a specified concentration of mercaptopropionic acid was placed on the other side. The dish was then cultured at 25°C for 2 days, and the growth of the Pythium bacteria was examined.
• Example 3 The growth inhibitory effect of mercaptopropionic acid on other plant pathogens was also examined. Fusarium oxysporum MAFF103054 strain was used as the Fusarium bacterium, and Ralstonia solanacearum MAFF301522 strain was used as the Ralstonia bacterium.
• Antibacterial tests were conducted on Fusarium bacteria using the dual culture method in the same manner as in Experimental Example 1. Additionally, the effect of the volatility of mercaptopropionic acid was investigated in the same manner as in Experimental Example 2. Fusarium bacteria were cultured on PDA solid medium at 25°C for 5 days.
• Ralstonia bacteria For Ralstonia bacteria, the formation of inhibition zones was examined using NA solid medium (4 g of blood agar base (Oxoid) and 0.5 g of yeast extract (Oxoid) per 100 mL). Ralstonia bacteria were cultured overnight in NYB liquid medium (5 g of nutrient broth (Oxoid) and 1 g of yeast extract (Oxoid) per 100 mL), and added to the NA solid medium at 10% (v/v) of the medium volume before the medium solidified, and mixed. After the medium solidified, filter paper soaked in a specified concentration of mercaptopropionic acid was placed near the center of the NA solid medium, and the medium was cultured at 25°C for 3 days.
• NA solid medium 4 g of blood agar base (Oxoid) and 0.5 g of yeast extract (Oxoid) per 100 mL.
• NYB liquid medium 5 g of nutrient broth (Oxoid) and 1 g of yeast
• Example 4 The effective concentration of mercaptopropionic acid was examined.
• the target plant pathogenic bacteria were Pythium and Ralstonia, and the Pythium ultimum MAFF425494 strain was used as the Pythium bacteria, and the Ralstonia solanacearum MAFF301522 strain was used as the Ralstonia bacteria.
• Ralstonia bacteria 1 mL of modified WSH medium was dispensed into a 24-well plate in the same manner as above, and 2 ⁇ L or 5 ⁇ L of Ralstonia solanacearum culture medium (overnight culture, no OD adjustment) was added, followed by culturing at 28°C and 100 rpm for 5 days.
• Example 5 A disease control test was carried out using a plant. Cucumber was used as the plant, and the disease control effect of mercaptopropionic acid was examined for an infectious disease caused by a soil pathogen, Pythium ultimum (Pythium ultimum MAFF425494 strain).
• 3-mercaptopropionic acid (Tokyo Chemical Industry Co., Ltd.) was used as the mercaptopropionic acid, and mercaptopropionic acid solutions were prepared to final concentrations of 2 mM and 5 mM. Mercaptopropionic acid solutions of various concentrations were then applied to the vermiculite prepared as described above. For comparison, tests were also conducted in which water alone, or ethanol with a final concentration of 2 mM or 5 mM was applied in place of mercaptopropionic acid in the same manner.
• the vermiculite doped with the mercaptopropionic acid solution or the various comparative samples was evenly divided into seedling trays (5 cm square, 5 cm deep per section) at 50 mL each. Three cucumber seeds were then sown in each seedling tray. The mercaptopropionic acid solution and the various comparative samples were both added within one day before sowing the cucumber seeds. The amount of mercaptopropionic acid solution and the various comparative samples added was 15 mL per section of the seedling tray.
• the seedling trays were placed in a plant incubator set at 25°C.
• the light period was set to 16 hours and the dark period to 8 hours, and the cultivation period in the incubator was two weeks.
• the number of surviving cucumbers two weeks after sowing was counted and evaluated.
• Mercaptopropionic acid solutions were prepared using 3-mercaptopropionic acid (Tokyo Chemical Industry Co., Ltd.) as mercaptopropionic acid, with final concentrations of 2 mM and 5 mM. Various concentrations of mercaptopropionic acid solutions were then irrigated at the base of tomato plants previously grown in vermiculite (1000-fold diluted Hyponex was used as a nutrient). Next, the roots of the tomato plants were cut with scissors, and 10 mL of bacterial wilt disease bacteria (Ralstonia solanacearum 8107S strain) adjusted to 2 x 10 8 cfu/mL was irrigated and cultivated at 30°C.
• 3-mercaptopropionic acid Tokyo Chemical Industry Co., Ltd.
• Various concentrations of mercaptopropionic acid solutions were then irrigated at the base of tomato plants previously grown in vermiculite (1000-fold diluted Hyponex was used as a nutrient). Next, the roots of
• the condition of tomato leaves was observed two days (2 dpi) and four days (4 dpi) after irrigation with the bacterial wilt pathogen. Specifically, the percentage of dead leaves in each tomato plant was examined, and the number of plants with dead leaves out of the total number of plants was examined.
• Cucumbers were grown in vermiculite under conditions of 18 hours light and 6 hours dark, while being fed 1000-fold diluted Hyponex as nutrients. About 3 weeks after sowing, the surfaces of the primary leaves of cucumbers were inoculated by spraying with Colletotrichum orbiculare (MAFF240422) adjusted to about 1 x 105 spores/mL. About 30 minutes after inoculation with the anthracnose fungus, 5 mM 3-mercaptopropionic acid solution or distilled water was sprayed on the leaves. The treated cucumbers were placed in a sealed container for about 4 days to maintain humidity, and then grown for 3 days with the container lid removed.
• Colletotrichum orbiculare MAFF240422
• the condition of the leaves of the grown cucumbers (7 days after inoculation with anthracnose (7 dpi)) was observed.
• a similar experiment was conducted in which the treated cucumbers were placed in an airtight container for about 4 days, and then the container was uncapped and grown for 7 days (11 days after inoculation with anthracnose (11 dpi)), and the condition of the leaves was also observed.
• the technique provided by the present invention is particularly useful in the agricultural field because it can suppress the growth of plant pathogens and protect plants from disease.
• the composition and method provided by the present invention can be used in the agricultural chemical field.

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