WO2024096475A1 - Cosmetic composition for pore care and suppression of sebum secretion comprising pomegranate flower extract as active ingredient - Google Patents

Abstract

The present invention provides a cosmetic composition for pore care and suppression of sebum secretion, comprising pomegranate flower extract as an active ingredient. As the pomegranate flower extract of the present invention is very rich in ellagic acid, which is an indicator compound, and has an excellent effect in suppressing sebum production, the pomegranate flower extract can be effectively used as a cosmetic for pore care and suppression of sebum secretion.

Description

Cosmetic composition for suppressing sebum secretion and pore care containing pomegranate flower extract as an active ingredient

This patent application claims priority to Korean Patent Application No. 10-2022-0144817 filed with the Korean Intellectual Property Office on November 2, 2022, the disclosure of which is incorporated herein by reference.

The present invention relates to a cosmetic composition that helps suppress excessive sebum secretion and prevent enlarged pores resulting from this. According to the present invention, the pomegranate flower extract obtained through ultrasonic extraction inhibits sebum synthesis in pores, thereby inhibiting excessive sebum production, thereby effectively preventing pores from widening.

Skin is a tissue that covers the surface of the body and protects the body from physical stimuli such as shock, heat, and sunlight. It also protects the body from chemical stimulation or bacterial invasion and plays a role in adapting the body to environmental changes, such as maintaining a constant body temperature. The skin is composed of the epidermis, dermis, and subcutaneous fat layers, and pores, which are passages that secrete sebum, are distributed throughout the facial skin. In particular, pores in the nose and cheek areas can be identified with the naked eye, and their appearance varies depending on various factors such as gender, genetics, aging, and acne. There are approximately 20,000 pores on the human face that secrete sebum. In general, the diameter of pores is very small, about 0.02 to 0.05 mm, so they are not easily visible, but they can expand for various reasons. Causes of enlarged pores include excessive sebum secretion, skin aging, and accumulation of waste products in pores. Sebum plays an important role in the body's defense by preventing moisture evaporation from the stratum corneum, but if secreted excessively, it makes the skin shiny and causes pores to expand and acne to form. Additionally, if waste products such as dust or makeup residue become trapped in enlarged pores, the pores become more prominent and the face looks messy. Therefore, many people are interested in reducing pores and managing sebum and pores through cosmetics.

The present inventors have made extensive research efforts to develop a material that can suppress excessive sebum secretion from the skin and prevent enlarged pores resulting from this. As a result, the present invention was completed by demonstrating that pomegranate flower extract using ultrasonic extraction contains a large amount of ellagic acid, an indicator component, and can effectively improve pore enlargement by controlling sebum secretion.

Therefore, the purpose of the present invention is to provide a cosmetic composition for suppressing sebum secretion and pore care containing pomegranate flower extract as an active ingredient.

Other objects and advantages of the present invention will become clearer from the following detailed description, claims, and drawings.

In order to solve the above-mentioned problem, the present inventors made extensive research efforts to develop a material that can suppress excessive sebum secretion from the skin and prevent pore enlargement resulting from this. As a result, the present invention was completed by confirming that pomegranate flower extract using ultrasonic extraction contains a large amount of ellagic acid, an indicator component, and can effectively improve pore enlargement by controlling sebum secretion.

According to one aspect of the present invention, the present invention provides a cosmetic composition for inhibiting sebum secretion and reducing pores containing pomegranate flower extract as an active ingredient.

In this specification, the pomegranate tree, which is a raw material plant, is a tree that began to be cultivated in Southwest Asia and the Mediterranean coast and is mainly produced in South Asia, such as Iran and Pakistan. The pomegranate tree is a deciduous tree with a height of about 3 to 5 m, and its leaves are about 7 cm long and have a glossy surface and a narrow oval shape. The flowers are bright red, have 3 to 7 petals, and are usually orange. The fruit is shaped like a round ball and has a calyx lobe at the end. The fruit skin is thick, and inside there are six fruiting chambers divided by thin septa, and numerous seeds are arranged along the septa.

The pomegranate flower extract used in the present invention can be purchased or obtained by direct extraction. When obtaining the pomegranate flower extract used in the composition of the present invention by treating it with an extraction solvent, various extraction solvents such as polar solvents or non-polar solvents can be used. Suitable polar solvents include (i) water, (ii) alcohol (preferably methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol) or ethylene glycol), (iii) acetic acid, (iv) dimethyl-formamide (DMFO), and (v) dimethylsulfoxide (DMSO). Suitable nonpolar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1- Pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloro Includes methane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, and THF (Tetrahydrofuran).

In one embodiment of the present invention, the pomegranate flower extract is extracted with any one extraction solvent selected from the group consisting of water, organic solvents, and mixed solvents thereof, and the organic solvent is alcohol, petroleum ether, hexane, benzene, Examples include chloroform, methylene chloride, ether, ethyl acetate, and acetone.

In one embodiment of the present invention, the alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, and mixed solvents thereof.

The amount of the solvent may vary depending on the amount of pomegranate flowers to be extracted, and may be specifically 1 to 20 times the volume of the weight of the pomegranate flowers, and more specifically 2 to 15 times the volume. More specifically, the volume is 3 to 12 times the weight of the pomegranate flower, and 4 to 10 times the volume, and more specifically, the volume is 4, 5, 6, 7, 8, 9, and 10 times the weight of the pomegranate flower, but is not limited thereto. .

In one embodiment of the present invention, the ethanol is an ethanol solution of 60 to 100% by volume. In a more specific embodiment of the present invention, the ethanol is 60 to 90% by volume, 60 to 80% by volume, 65 to 100% by volume, 65 to 90% by volume, 65 to 80% by volume, 70% by volume, 80% by volume. , may be 90% by volume, 95% by volume, or 100% by volume, but is not limited thereto.

The extraction temperature of the extract of the present invention is not particularly limited, and may be, for example, 0°C to 120°C, specifically 10°C to 95°C. More specifically, 15 ℃ to 70 ℃, 15 ℃ to 60 ℃, 15 ℃ to 50 ℃, 15 ℃ to 40 ℃, 15 ℃ to 30 ℃, 15 ℃ to 25 ℃, 20 ℃ to 70 ℃, 20 ℃ to 60 ℃ ℃, 20℃ to 50℃, 20℃ to 40℃, 20℃ to 30℃, 20℃ to 25℃, most specifically 10℃, 15℃, 20℃, 25℃, 30℃, 40℃, 50℃ , 60°C, or 70°C, but is not limited thereto.

The extract of the present invention can be extracted using a known natural product extraction method. For example, it can be extracted by cold needle extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, or heating extraction. Specifically, it can be extracted by hot water extraction, ultrasonic extraction, or reflux cooling extraction, and can be extracted 1 to 10 times, more Specifically, extraction can be repeated 2 to 7 times.

In a specific embodiment of the present invention, the pomegranate flower extract may be extracted using an ultrasonic extractor.

The ultrasonic extraction is performed at a frequency of 10 to 100 kHz for 1 to 12 hours. The frequencies are more specifically 10 to 90 kHz, 10 to 80 kHz, 10 to 70 kHz, 10 to 60 kHz, 10 to 50 kHz, 10 to 40 kHz, 10 to 35 kHz, 10 to 30 kHz, 10 to 25 kHz. , 10 to 20 kHz, 20 to 90 kHz, 20 to 80 kHz, 20 to 70 kHz, 20 to 60 kHz, 20 to 50 kHz, 20 to 40 kHz, 20 to 35 kHz, 20 to 30 kHz, 20 to 25 kHz , 30 to 90 kHz, 30 to 80 kHz, 30 to 70 kHz, 30 to 60 kHz, 30 to 50 kHz, 30 to 40 kHz, 30 to 35 kHz, 40 to 90 kHz, 40 to 80 kHz, 40 to 70 kHz , 40 to 60 kHz, 40 to 50 kHz, 20 kHz, 25 kHz, 30 kHz, 35 kHz, 40 kHz, 45kHz, or 50 kHz, but is not limited thereto.

In one embodiment of the present invention, the concentration of pomegranate flower extract in the cosmetic composition is about 0.01 to 10% (g/g), about 0.05 to 10% (g/g), and about 0.1 to 10% (g/g). , about 0.3 to 10% (g/g), about 0.5 to 10% (g/g), about 0.7 to 10% (g/g), about 0.8 to 10% (g/g), about 0.9 to 10% (g/g), about 1 to 10% (g/g), about 1.5 to 10% (g/g), about 2 to 10% (g/g), about 3 to 10% (g/g), About 4 to 10% (g/g), about 5 to 10% (g/g), about 6 to 10% (g/g), about 7 to 10% (g/g), about 8 to 10% ( g/g), about 9 to 10% (g/g), about 0.01 to 8% (g/g), about 0.05 to 8% (g/g), about 0.1 to 8% (g/g), about 0.3 to 8% (g/g), about 0.5 to 8% (g/g), about 0.7 to 8% (g/g), about 0.8 to 8% (g/g), about 0.9 to 8% (g/g) /g), about 1 to 8% (g/g), about 1.5 to 8% (g/g), about 2 to 8% (g/g), about 3 to 8% (g/g), about 4 to 8% (g/g), about 5 to 8% (g/g), about 6 to 8% (g/g), about 7 to 8% (g/g), about 0.01 to 7% (g/g) g), about 0.05 to 7% (g/g), about 0.1 to 7% (g/g), about 0.3 to 7% (g/g), about 0.5 to 7% (g/g), about 0.7 to 7% (g/g), about 0.8 to 7% (g/g), about 0.9 to 7% (g/g), about 1 to 7% (g/g), about 1.5 to 7% (g/g) ), about 2 to 7% (g/g), about 3 to 7% (g/g), about 4 to 7% (g/g), about 5 to 7% (g/g), about 6 to 7 % (g/g), about 0.01 to 6% (g/g), about 0.05 to 6% (g/g), about 0.1 to 6% (g/g), about 0.3 to 6% (g/g) , about 0.5 to 6% (g/g), about 0.7 to 6% (g/g), about 0.8 to 6% (g/g), about 0.9 to 6% (g/g), about 1 to 6% (g/g), about 1.5 to 6% (g/g), about 2 to 6% (g/g), about 3 to 6% (g/g), about 4 to 6% (g/g), About 5 to 6% (g/g), about 0.01 to 5% (g/g), about 0.05 to 5% (g/g), about 0.1 to 5% (g/g), about 0.3 to 5% ( g/g), about 0.5 to 5% (g/g), about 0.7 to 5% (g/g), about 0.8 to 5% (g/g), about 0.9 to 5% (g/g), about 1 to 5% (g/g), about 1.5 to 5% (g/g), about 2 to 5% (g/g), about 3 to 5% (g/g), about 4 to 5% (g/g) /g), about 0.01 to 4% (g/g), about 0.05 to 4% (g/g), about 0.1 to 4% (g/g), about 0.3 to 4% (g/g), about 0.5 to 4% (g/g), about 0.7 to 4% (g/g), about 0.8 to 4% (g/g), about 0.9 to 4% (g/g), about 1 to 4% (g/g) g), about 1.5 to 4% (g/g), about 2 to 4% (g/g), about 3 to 4% (g/g), about 0.01 to 3% (g/g), about 0.05 to 3% (g/g), about 0.1 to 3% (g/g), about 0.3 to 3% (g/g), about 0.5 to 3% (g/g), about 0.7 to 3% (g/g) ), about 0.8 to 3% (g/g), about 0.9 to 3% (g/g), about 1 to 3% (g/g), about 1.5 to 3% (g/g), about 2 to 3 % (g/g), about 0.01 to 2% (g/g), about 0.05 to 2% (g/g), about 0.1 to 2% (g/g), about 0.3 to 2% (g/g) , about 0.5 to 2% (g/g), about 0.7 to 2% (g/g), about 0.8 to 2% (g/g), about 0.9 to 2% (g/g), about 1 to 2% (g/g), about 1.5 to 2% (g/g), about 0.01 to 1% (g/g), about 0.05 to 1% (g/g), about 0.1 to 1% (g/g), About 0.3 to 1% (g/g), about 0.5 to 1% (g/g), about 0.7 to 1% (g/g), about 0.8 to 1% (g/g), or about 0.9 to 1% (g/g), but is not limited thereto.

In one embodiment of the present invention, the pomegranate flower extract contains ellagic acid.

In one embodiment of the present invention, the pomegranate flower extract contains ellagic acid at a concentration of 300 to 600 ppm. More specifically, 300 to 600 ppm, 350 to 600 ppm, 360 to 600 ppm, 370 to 600 ppm, 380 to 600 ppm, 390 to 600 ppm, 400 to 600 ppm, 410 to 600 ppm, 420 to 600 ppm, 430 ppm. to 600 ppm, 440 to 600 ppm, 450 to 600 ppm, 460 to 600 ppm, 470 to 600 ppm, 480 to 600 ppm, 490 to 600 ppm, 500 to 600 ppm, 300 to 550 ppm, 350 to 550 ppm, 360 to 550 ppm, 370 to 550 ppm, 380 to 550 ppm, 390 to 550 ppm, 400 to 550 ppm, 410 to 550 ppm, 420 to 550 ppm, 430 to 550 ppm, 440 to 550 ppm, 450 to 550 ppm, 460 to 550 ppm, 470 to 550 ppm, 480 to 550 ppm, 490 to 550 ppm, 500 to 550 ppm, 300 to 500 ppm, 350 to 500 ppm, 360 to 500 ppm, 370 to 500 ppm, 380 to 500 ppm, 390 to 500 ppm, 400 to 500 ppm, 410 to 500 ppm, 420 to 500 ppm, 430 to 500 ppm, 440 to 500 ppm, 450 to 500 ppm, 460 to 500 ppm, 470 to 500 ppm, 480 to 500 ppm, or It may be included at a concentration of 490 to 500 ppm, but this is only an example and not limited thereto, and the content of the extract in the composition may be adjusted depending on the necessary sebum secretion inhibition and pore reduction effects.

The term ‘extract’ used in this specification has the meaning commonly used in the art as a crude extract, as described above, but in a broad sense also includes fractions obtained by additional fractionation of the extract. In other words, the extract of pomegranate flowers includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity, or affinity), etc. Fractions obtained through purification methods are also included in the pomegranate flower extract of the present invention.

The pomegranate flower extract used in the present invention can be prepared in powder form by additional processes such as reduced pressure distillation and freeze-drying or spray drying.

In one embodiment of the present invention, the composition of the present invention may include not only the pomegranate flower extract, which is an active ingredient, but also ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, and pigments. and conventional auxiliaries such as flavorings, and carriers.

As the carrier, purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butylene glycol or propylene glycol), higher fatty acids (palmitic acid or linolenic acid), oils and fats (wheat germ oil, Camellia oil, jojoba oil, olive oil, squalane, sunflower oil, macadamia peanut oil, Avogard oil, hydrogenated soybean lecithin or fatty acid glycerides) can be used, but are not limited to these. Additionally, surfactants, disinfectants, antioxidants, ultraviolet absorbers, anti-inflammatory agents, and fresheners can be added as needed.

Surfactants include polyoxyethylene, hydrogenated castor oil, polyoxyethylene, oleyl ether, polyoxyethylene monooleate, polyoxyethylene, glyceryl monostearate, sorbitan monostearate, polyoxyethylene monooleate, sorbitan, Sucrose fatty acid ester, hexaglycerin monolauric acid, polyoxyethylene reduced lanolin, POE, glyceryl pyroglutamic acid, isostearic acid, diester, N-acetylglutamine, and isostearyl ester can be used.

Disinfectants include hinocthiol, tricrosan, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, and zinc pyritaone.

As an antioxidant, any of butylhydroxyanisole, gallic acid, propyl gallic acid, and erythorbic acid can be used.

Ultraviolet absorbers include benzophenones such as dihydroxybenzophenone, melanin, ethyl para-aminobenzoate, 2-ethylhexyl para-dimethylaminobenzoic acid, synoxite, para-methoxycinnamic acid 2-ethylhexyl ester, 2-(2- Hydroxy-5-methylphenyl) benzotriazole, urochanic acid, and metal oxide fine particles can be used.

As an anti-inflammatory agent, dipotassium glycyrrhizinate or allantoin can be used, and as a refreshing agent, red pepper tincture or 1-menthol can be used.

In another embodiment of the present invention, the cosmetic composition of the present invention is used as an external ointment, gel, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, skin ampoule, hair ampoule, and scalp treatment. , hair tonic, hair conditioner, hair treatment, hair lotion, hair shampoo, hair rinse, conditioner shampoo, nourishing hair lotion, hair gel, hair wax, hair spray, hair dye, soap, liquid detergent, bath salt, sunscreen cream. , sun oil, suspension, emulsion, paste, skin lotion, powder, skin cleansing, powder foundation, emulsion foundation, wax foundation, patch and spray, but is not limited thereto. .

As demonstrated in specific embodiments of the present invention, it was confirmed that the content of the indicator component ellagic acid in the pomegranate flower extract, which is an active ingredient of the cosmetic composition of the present invention, was increased in the extract prepared by ultrasonic extraction compared to the extract prepared by solvent extraction. .

In addition, it was confirmed that the content of ellagic acid increased in the extract of pomegranate flowers compared to the extract using pomegranate fruit.

In addition, as demonstrated in specific embodiments of the present invention, the cosmetic composition of the present invention has the effect of suppressing sebum secretion by inhibiting sebum synthesis in pores. The sebum synthesis inhibitory effect is concentration-dependent of the pomegranate flower ultrasonic extract.

Additionally, the cosmetic composition of the present invention has the effect of reducing the number and area of pores when applied to the skin.

From the above results, it can be seen that the pomegranate flower extract of the present invention helps to reduce skin shine or reduce pores caused by excessively secreted sebum.

According to another aspect of the present invention, the present invention provides a method of inhibiting sebum secretion from the skin, comprising the step of applying the above-described cosmetic composition to the skin of a subject.

Since the method for inhibiting sebum secretion from the skin includes the step of applying or administering the cosmetic composition according to one aspect of the present invention, the common content between both inventions equally applies to the method for inhibiting sebum secretion.

In one embodiment of the present invention, the “subject” refers to a mammal. The mammal may be a human, mouse, rat, hamster, ferret, guinea pig, rabbit, dog, cat, pig, cow, horse, sheep, goat, or non-human primate, and is most specifically a human.

The features and advantages of the present invention are summarized as follows:

(i) The present invention provides a cosmetic composition for suppressing sebum secretion and pore care containing pomegranate flower extract as an active ingredient.

(ii) The present invention provides a method of inhibiting sebum secretion from the skin, comprising the step of applying the cosmetic composition to the skin of a subject.

(iii) The pomegranate flower extract of the present invention is very rich in ellagic acid, an indicator component, and has an excellent effect of suppressing sebum production, so it can be usefully used as a pore care cosmetic.

Figure 1 is a diagram showing the structure and HPLC chromatogram of ellagic acid selected as an indicator component of the pomegranate flower extract of the present invention.

Figure 2 is a diagram showing chromatograms obtained as a result of HPLC screening of pomegranate fruit extract, pomegranate fruit ultrasonic extract, pomegranate flower extract, and pomegranate flower ultrasonic extract at the same concentration according to Comparative Examples 1 and 2 and Examples 1 and 2 of the present invention.

Figure 3 is a diagram showing the sebum synthesis inhibitory activity in pores of pomegranate fruit ultrasonic extract and pomegranate flower ultrasonic extract according to Comparative Example 2 and Example 2 of the present invention.

Figure 4 is a diagram showing the sebum synthesis inhibitory activity in pores of ellagic acid, which was set as an indicator component in comparative examples and examples of the present invention.

Figure 5 is a diagram showing that the number and area of pores decrease over time when Formulation Example 1 and Formulation Example 2 of the present invention are applied.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example

Throughout this specification, “%” used to indicate the concentration of a specific substance means (weight/weight) % for solid/solid, (weight/volume) % for solid/liquid, and Liquid/liquid is (volume/volume) %.

Example 1: Preparation of pomegranate flower extract

40 g of dried pomegranate flowers were immersed in 400 g of 70% ethanol and extracted at 70°C for 3 hours, then filtered through filter paper (Whatman No. 2) and concentrated to obtain a powder. The concentrated powder was dissolved in purified water to a concentration of 1.0% (g/g), filtered through filter paper (Durapore 0.22㎛, PVDF), and used in the test.

Example 2: Preparation of pomegranate flower ultrasonic extract

40 g of dried pomegranate flowers were immersed in 400 g of 70% ethanol and extracted using an ultrasonic extractor at room temperature. Extraction was performed at 40 kHz for 3 hours, then filtered through filter paper (Whatman No. 2) and concentrated to obtain a powder. The concentrated powder was dissolved in purified water to a concentration of 1.0% (g/g), filtered through filter paper (Durapore 0.22㎛, PVDF), and used in the test.

Comparative Example 1: Preparation of pomegranate fruit extract

40 g of dried pomegranate fruit was immersed in 400 g of 70% ethanol and extracted at 70°C for 3 hours, then filtered through filter paper (Whatman No. 2) and concentrated to obtain a powder. The concentrated powder was dissolved in purified water to a concentration of 1.0% (g/g), then filtered through filter paper (Durapore 0.22㎛, PVDF) before use.

Comparative Example 2: Preparation of pomegranate fruit ultrasonic extract

40 g of dried pomegranate fruit was immersed in 400 g of 70% ethanol and extracted using an ultrasonic extractor at room temperature. Extraction was performed at 40 kHz for 3 hours, then filtered through filter paper (Whatman No. 2) and concentrated to obtain a powder. The obtained concentrated powder was dissolved in purified water so that the concentration was 1.0% (g/g), then filtered through filter paper (Durapore 0.22㎛, PVDF) before use.

Experimental Example 1: Confirmation of the content of the indicator component ellagic acid

The present inventors selected ellagic acid as an indicator component representing the extract among the various chemical compounds contained in the pomegranate flower extract, which is the active ingredient of the present invention. Accordingly, the content of ellagic acid in the extracts extracted through Examples 1 and 2 and Comparative Examples 1 and 2 described above was confirmed through HPLC. Figure 1 shows the structure, chemical formula, molecular weight, and HPLC chromatogram of ellagic acid.

Specifically, the same amount of extract for each example and comparative example was used for analysis. The ellagic acid used in the analysis was diluted according to the concentration ratio using methanol, and quantitative analysis of two types of comparative examples and two types of examples was performed using the calibration curve obtained by making three standard solutions.

For HPLC screening, analysis conditions were set as follows. The column used was a C18-reverse phase column (Aegispak C18-L, 5㎛, 4.6mm x 250mm), and solvent A (water containing 0.1% (v/v) formic acid) and solvent B (acetonitrile) were used. . Solvent A was stabilized by flowing up to 90%, and then slowly changed to 50% over 30 minutes. The flow rate was set at 1 mL/min, and the column temperature was maintained at 35°C for analysis. The sample was analyzed using an ultraviolet absorptiophotometer at 260 nm.

The results are shown in Figure 2 and Table 2.

As shown in Figure 2, as a result of HPLC screening of Comparative Examples 1 and 2 and Examples 1 and 2 at the same concentration, the peak at 15.2 minutes corresponding to ellagic acid was higher than that of pomegranate flowers than the extracts of Comparative Examples 1 and 2 using pomegranate fruits. It was confirmed that it was contained at a higher concentration in the extracts of Examples 1 and 2 using . In addition, a higher concentration of ellagic acid was confirmed in the extract of Example 2 using ultrasound than in the extract of Example 1 using pomegranate flowers.

Specifically, as shown in Table 1 below, it was confirmed that the ellagic acid content in the extract of Example 1 was about 1.9 times higher than that of the extract of Comparative Example 1. Additionally, when comparing Example 1 and Example 2, it was confirmed that the ellagic acid content in the extract of Example 2 using ultrasound was about 1.2 times higher.

part	Ellagic acid (ppm)
Comparative Example 1 (general fruit extraction)	218.62 ± 36.83
Comparative Example 2 (ultrasonic extraction of fruit)	249.39 ± 15.53
Example 1 (General flower extract)	409.94 ± 17.22
Example 2 (Flowers Ultrasonic Extraction)	489.06 ± 11.92

From the above results, the present inventors confirmed that the concentration of ellagic acid was higher in pomegranate flowers than in pomegranate fruits in terms of raw materials, and that depending on the extraction method, ultrasonic extraction had a higher concentration of ellagic acid than general solvent extraction.

Experimental Example 2: Inhibitory effect of pomegranate ultrasonic extract on fatty acid synthase gene expression

The inhibitory effect on fatty acid synthase (FAS) gene expression of ultrasonic extract with relatively high ellagic acid content was confirmed. The following experiment was conducted to evaluate whether Comparative Example 2 and Example 2 were active in suppressing excessive sebum secretion by inhibiting FAS, a factor involved in sebum synthesis.

Inoculate and culture human normal sebocytes in a 60 mm dish. Replace with serum-free medium, treat DHT and sample together, and culture in 37°C, 5% CO ₂ incubator. After removing the medium, cells were collected with QIAzol ^TM Lysis Reagent (Qiagen) and RNA was extracted according to the manufacturer's method. After quantifying the extracted RNA, cDNA was synthesized and real-time PCR (Applied Biosystems, 7500 FAST Real-Time PCR System) was performed to confirm FAS (Fatty acid synthase) gene expression. The primers used in the PCR were synthesized by Cosmogenetec (Korea), and the primer sequences are shown in Table 2.

division	direction	Base sequence (5’ to 3’)	SEQ ID NO:
Fatty acid synthase (FAS)	Forward	CGT CCT CTT CCC CGC CAC	One
	Reverse	GCA CCA CAT CCT CAA ACA CCA CA	2
β-actin	Forward	GGC ACC CAG CAC AAT GAA G	3
	Reverse	CCG ATC CAC ACG GAG TAC TTG	4

The results of an experiment evaluating the inhibition of FAS gene expression of the pomegranate fruit ultrasonic extract according to Comparative Example 2 and the pomegranate flower ultrasonic extract according to Example 2 are shown in Figure 3.

As shown in Figure 3, the pomegranate flower ultrasonic extract of Example 2 inhibited FAS from 0.001%, and an inhibition rate of up to 43.8% was confirmed. Comparative Example 2 also inhibited FAS from 0.001%, but showed lower activity than Example 2 at a maximum of 28.7%.

Experimental Example 3: Inhibitory effect of fatty acid synthase gene expression of indicator ingredient ellagic acid

In order to evaluate whether the ellagic acid contained as an indicator component in Comparative Example 2 and Example 2 is active in suppressing excessive sebum secretion by inhibiting sebum synthesis in pores, an experiment was performed in the same manner as in Experimental Example 2, except that the Concentrations were tested at 1, 2.5, and 5 μg/mL, respectively.

The results are shown in Figure 4.

As shown in Figure 4, the expression of FAS was inhibited starting from 1 μg/mL, and at 2.5 μg/mL, an inhibition rate of FAS gene expression of up to 39.0% was confirmed.

Experimental Example 4: Simple clinical trial for pore care of pomegranate ultrasonic extract

To evaluate whether the extracts of Comparative Example 2 and Example 2 were effective in pore care, a simple clinical test was conducted using full-eye imaging as follows.

For a simple pore care clinical trial, Formulation Example 1 and Formulation Example 2 were prepared and used by applying Example 2 and Comparative Example 2, respectively, as shown in Table 3.

Ingredient name	content (%)
	Formulation Example 1	Formulation Example 2
Purified water	82.85	82.85
Butylene glycol	10.00	10.00
glycerin	3.00	3.00
1,2-hexanediol	1.60	1.60
Bis-PEG-18methyletherdimethylsilane	0.50	0.50
PEG-60 Hydrogenated Castor Oil	0.30	0.30
Betaine	0.20	0.20
carbomer	0.20	0.20
Tromethamine	0.16	0.16
allantoin	0.10	0.10
Ethylhexylglycerin	0.05	0.05
Disodium EDTA	0.02	0.02
Spices	0.02	0.02
Example 2 (Pomegranate Flower Ultrasonic Extract)	1.00	-
Comparative Example 2 (Pomegranate Fruit Ultrasonic Extract)	-	1.00
Sum	100.00	100.00

The test subjects were four men and women over the age of 30, and the test was conducted after resting for 30 minutes after washing the face. Changes in skin pores were measured by photographing the entire face using a Cosmetic Full-Face Photography System (Facial Stage DM-3; MORITEX Corp., Japan). To ensure consistent measurement, the experiment was conducted by one person in charge of the experiment in the same position under uniform lighting, and all subjects' faces were photographed with the front view fixed. The device was photographed before, immediately after, and after 2 hours of use of the sample, and the photographed results were used to derive analysis values of the number and area of skin pores contained in a designated random area of both cheeks using DM-3 Manager. After that, the improvement rate was calculated by substituting it into the following equation. It was judged that the greater the improvement rate, the greater the improvement effect.

ceremony

Improvement rate (%) =

The results of pore number and area reduction rate through whole-eye imaging of pomegranate flower ultrasonic extract and pomegranate fruit ultrasonic extract according to Formulation Example 1 and Formulation Example 2 are shown in Figure 5.

As shown in Figure 5, when Formulation Example 1 was applied, the number of pores decreased immediately after application, and over time, it showed about three times better activity than Formulation Example 2. Additionally, when Formulation Example 1 was applied, it was confirmed that the pore area was reduced by about 6.8%. On the other hand, Formulation Example 2 reduced the number of pores, but it was confirmed that the effect of reducing the pore area was minimal.

From the above results, it was found that the pore care efficacy of the ultrasonic extract made from pomegranate flowers was superior to that of pomegranate fruit.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred implementation examples and do not limit the scope of the present invention.

Claims (9)

1. A cosmetic composition for suppressing sebum secretion and reducing pores containing pomegranate flower extract as an active ingredient.
2. The cosmetic composition according to claim 1, wherein the extract is extracted with any one extraction solvent selected from the group consisting of water, organic solvents, and mixed solvents thereof.
3. The cosmetic composition of claim 2, wherein the organic solvent is selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, petroleum ether, hexane, benzene, chloroform, methylene chloride, ether, ethyl acetate, and acetone.
4. The cosmetic composition of claim 1, wherein the pomegranate flower extract is extracted using an ultrasonic extractor.
5. The cosmetic composition of claim 4, wherein the ultrasonic extraction is performed at a frequency of 10 to 100 kHz for 1 to 12 hours.
6. The cosmetic composition of claim 1, wherein the pomegranate flower extract contains ellagic acid.
7. The cosmetic composition of claim 1, wherein the cosmetic composition suppresses sebum secretion by inhibiting sebum synthesis in pores.
8. The cosmetic composition of claim 1, wherein the cosmetic composition reduces the number and area of pores.
9. The cosmetic composition according to claim 1, wherein the cosmetic composition includes external ointment, gel, cream, foam, nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, skin ampoule, hair ampoule, scalp treatment, hair tonic, and hair. Conditioner, hair treatment, hair lotion, hair shampoo, hair rinse, conditioner shampoo, nourishing hair lotion, hair gel, hair wax, hair spray, hair dye, soap, liquid detergent, bath salt, sunscreen cream, sun oil, suspension. A cosmetic composition prepared in a formulation selected from the group consisting of emulsion, paste, skin lotion, powder, skin cleansing, powder foundation, emulsion foundation, wax foundation, patch and spray.

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