WO2024087107A1 - Contenant de biopuce et ses procédés de préparation et d'utilisation - Google Patents

Contenant de biopuce et ses procédés de préparation et d'utilisation Download PDF

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Publication number
WO2024087107A1
WO2024087107A1 PCT/CN2022/127996 CN2022127996W WO2024087107A1 WO 2024087107 A1 WO2024087107 A1 WO 2024087107A1 CN 2022127996 W CN2022127996 W CN 2022127996W WO 2024087107 A1 WO2024087107 A1 WO 2024087107A1
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WIPO (PCT)
Prior art keywords
chip
biochip
container
container body
biological sample
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PCT/CN2022/127996
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English (en)
Chinese (zh)
Inventor
于群
洪艳
李泉水
陈奥
黎宇翔
章文蔚
徐讯
Original Assignee
深圳华大生命科学研究院
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Application filed by 深圳华大生命科学研究院 filed Critical 深圳华大生命科学研究院
Priority to PCT/CN2022/127996 priority Critical patent/WO2024087107A1/fr
Publication of WO2024087107A1 publication Critical patent/WO2024087107A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Definitions

  • Embodiments of the present disclosure generally relate to the field of biochemical technology, and more specifically, to biochip containers that can accommodate chips of non-standard sizes and ensure that the chips are not damaged while performing automated operations such as imaging, liquid replacement, and transfer on a biochemical analysis instrument. Embodiments of the present disclosure also relate to methods for preparing and using biochip containers.
  • chip carrier solution that can accommodate chips of unconventional cutting sizes and ensure that the chip loaded with biological samples can be automatically processed on the instrument without damaging the chip while taking into account automated operations such as imaging, fluid replacement, and transfer on the instrument.
  • the present disclosure is proposed to overcome at least one of the above and other problems and drawbacks of the prior art.
  • a biochip container comprising: a container body, which is formed with a groove, the groove defining a accommodating space, the accommodating space is suitable for accommodating a chip, and a biological sample to be analyzed will be loaded on the chip; the biochip container has a transparent part, which is basically aligned with the groove in the thickness direction and covers the groove to allow the biological sample on the chip accommodated in the accommodating space to be photographed through the transparent part.
  • the biochip container further comprises: a cover glass disposed on a surface of the container body, the cover glass comprising the transparent portion.
  • the cover glass is formed with a through hole, which is connected to the fluid of the receiving space to allow at least one of the following operations to be performed through the through hole: adding reaction liquid to the receiving space; extracting waste liquid from the receiving space; and recovering reaction products from the receiving space.
  • the through-holes include a first through-hole and a second through-hole penetrating the cover glass in the thickness direction, and each of the first through-hole and the second through-hole is configured to allow one or more of the operations to be performed.
  • the first through hole and the second through hole are disposed in a portion of the cover glass that is located outside the groove in a direction perpendicular to the thickness direction.
  • the first through hole and the second through hole are located on opposite sides of the slot in a direction perpendicular to the thickness direction.
  • the first through hole and the second through hole have the same or different opening shapes.
  • the biochip container also includes a glass slide having a chip positioning area on a surface of one side, the chip is suitable for being fixed in the chip positioning area, the container body is suitable for being sealed and assembled on the surface of the glass slide, and the groove runs through the container body in the thickness direction and is substantially aligned with the chip positioning area.
  • the container body is suitable for being sealed to the surface of the slide by an adhesive or a fastener; and/or the cover glass is suitable for being sealed to the surface of the container body facing away from the slide by an adhesive or a fastener.
  • the groove comprises a bottomed groove formed in the container body, the bottomed groove being open at a side facing the cover glass.
  • the biochip container also includes a glass slide having a chip positioning area on a surface of one side, the chip is suitable for being fixed in the chip positioning area, the container body is suitable for being sealed and assembled on the surface of the glass slide, the groove penetrates the container body in the thickness direction and is substantially aligned with the chip positioning area, and the transparent part is formed as one piece with the container body or is a part of the container body.
  • the biochip container further comprises the chip fixed on a glass slide.
  • the receiving space defined by the groove is also suitable for receiving a reaction liquid, wherein the reaction liquid includes a reaction reagent or a cleaning reagent that will undergo a biochemical reaction with a biological sample on the chip.
  • the size of the slot is larger than the size of the chip.
  • the slot has a shape that substantially matches the shape of the chip.
  • the wall thickness of the groove is greater than the thickness of the chip.
  • the container body is further formed with an opening, which is fluidically connected to the holding space to allow at least one of the following operations to be performed through the opening: adding reaction liquid to the holding space; extracting waste liquid from the holding space; and recovering reaction products from the holding space.
  • the container body is further formed with an opening, which is substantially aligned with the through hole in the thickness direction and is in fluid communication with both the accommodating space and the through hole.
  • the opening includes a first opening and a second opening located on different sides of the slot.
  • the container body is made of flat glass or rubber material.
  • the container body is formed with a plurality of slots having the same or different sizes.
  • the receiving space is configured to receive a chip having a size ranging from 1 cm to 13 cm.
  • an embodiment provides a method for preparing a biochip container, comprising the following steps: providing a glass slide, a flat carrier and a cover glass; processing the flat carrier as a container body to form a groove in the flat carrier, the groove defining a receiving space suitable for receiving a chip, and the biological sample to be analyzed will be loaded on the chip; assembling the cover glass on the container body so that at least a transparent portion of the cover glass covers the groove; and after the chip is fixed on the glass slide and the biological sample to be analyzed is loaded on the chip, sealingly assembling the assembled cover glass and the container body to the glass slide, so that the chip loaded with the biological sample to be analyzed is received in the receiving space.
  • an embodiment provides a method for preparing a biochip container, comprising the following steps: providing a flat carrier and a cover glass; processing the flat carrier as a container body to form a bottom groove in the flat carrier, the bottom groove defining a receiving space suitable for receiving a chip, and the biological sample to be analyzed will be loaded on the chip; and after the chip loaded with the biological sample to be analyzed is received in the receiving space, assembling the cover glass onto the container body, so that at least the transparent portion of the cover glass covers the bottom groove.
  • an embodiment provides a method for preparing a biochip container, comprising the following steps: providing a glass slide and a flat plate carrier; processing the flat plate carrier as a container body to form a bottom groove in the flat plate carrier, the bottom groove defining a receiving space suitable for receiving a chip, the biological sample to be analyzed will be loaded on the chip, and the bottom of the bottom groove is a transparent portion; and after the chip is fixed on the glass slide and the biological sample to be analyzed is loaded on the chip, sealingly assembling the container body to the glass slide, so that the chip loaded with the biological sample to be analyzed is received in the receiving space to allow the biological sample to be photographed through the transparent portion.
  • the method for preparing a biochip container further comprises the steps of: providing the chip; and fixing the chip in a chip positioning region of a glass slide before the container body is sealingly assembled onto the glass slide.
  • the method for preparing a biochip container also includes the following steps: forming a through hole in the cover glass, wherein the through hole is connected to the fluid of the receiving space after the cover glass is assembled onto the flat carrier, so as to allow at least one of the following operations to be performed through the through hole: adding reaction liquid to the receiving space; extracting waste liquid from the receiving space; and recovering reaction products from the receiving space.
  • the step of "processing the flat plate carrier as a container body” also includes: forming an opening in the flat plate carrier, wherein the opening is fluidly connected to the holding space to allow at least one of the following operations to be performed through the opening: adding reaction liquid to the holding space; extracting waste liquid from the holding space; and recovering reaction products from the holding space.
  • an embodiment provides a method for analyzing a biological sample, comprising: providing a biochip container described in any embodiment of the present disclosure; fixing a chip in the biochip container and loading a biological sample on the chip; and performing at least one of the following operations: clamping or adsorbing the biochip container containing the chip loaded with the biological sample to place the biochip container in or remove it from an analytical instrument; taking a picture of the biological sample in the biochip container in the analytical instrument; and performing pipetting, waste liquid extraction, biochemical reactions, and product recovery in the biochip container in the analytical instrument.
  • the operations are performed in a spatial omics or spatiotemporal omics operation instrument.
  • FIG1 is a perspective view schematically showing the structure of a biochip container according to an exemplary embodiment of the present disclosure
  • FIG2 is an exploded view schematically showing the structure of a biochip container according to an exemplary embodiment of the present disclosure
  • FIG3 is a top view schematically showing the structure of a biochip container according to an exemplary embodiment of the present disclosure
  • FIG4 is a cross-sectional view taken along line A-A in FIG3 , schematically illustrating the structure of a portion of a biochip container according to an exemplary embodiment of the present disclosure
  • FIG. 5 is a perspective view schematically showing the structure of a biochip container according to an exemplary embodiment of the present disclosure, with a cover glass removed;
  • FIG. 6 is a perspective view schematically showing the structure of a container body of a biochip container according to an exemplary embodiment of the present disclosure.
  • FIG. 7 is a perspective view schematically showing the structure of a slide glass carrying a chip of a biochip container according to an exemplary embodiment of the present disclosure
  • FIG8 is a perspective view schematically showing the structure of a biochip container according to another exemplary embodiment of the present disclosure.
  • FIG9 is a cross-sectional view taken along line B-B in FIG8 , schematically showing the structure of a portion of a biochip container;
  • FIG. 10 is a perspective view schematically showing the structure of a biochip container according to yet another exemplary embodiment of the present disclosure.
  • FIG11 is a cross-sectional view taken along line C-C in FIG10 , schematically showing the structure of a portion of a biochip container;
  • FIG. 12 is a flow chart schematically illustrating a method for preparing a biochip container according to an exemplary embodiment of the present disclosure
  • FIG. 13 is a flow chart schematically illustrating a method for preparing a biochip container according to another exemplary embodiment of the present disclosure.
  • FIG. 14 is a flow chart schematically illustrating a method for preparing a biochip container according to yet another exemplary embodiment of the present disclosure.
  • a biochip container which includes a container body, the container body is formed with a groove, the groove defines a storage space, and the chip loaded with the biological sample to be analyzed is suitable for being accommodated in the storage space of the container body, and the biochip container (such as its cover glass or container body) also has a transparent part, which is substantially aligned with the groove in the thickness direction of the biochip container and covers the groove or the container space, so as to allow the biological sample on the chip accommodated in the storage space to be photographed through the transparent part.
  • This biochip container can not only accommodate chips of non-standard sizes, but also ensure that the chip is not damaged while performing automated operations such as imaging, liquid replacement, and transfer on the analysis instrument.
  • the biological samples described in this article may include but are not limited to pathological samples, tissue samples, cell samples, etc., and more specifically, may be tissue sections, pathological sections, etc.
  • the biochip container 100 mainly includes a glass slide 110, a container body 120, and a cover glass 130 which are sequentially stacked in the thickness direction Z.
  • the glass slide 110 has a chip positioning area 111 on the surface of one side thereof, and the chip positioning area 111 may be a flat area or a concave area.
  • the biochip 1 is suitable for being fixed in the chip positioning area 111, for example, the chip 1 may be attached to the chip positioning area 111 or other suitable positions on the glass slide by an adhesive or glue that does not affect the subsequent biochemical reaction on the chip.
  • the container body 120 is formed with a slot 121, which defines a receiving space, and the chip 1 is suitable for being at least partially received in the slot 121 or the receiving space, or the chip 1 can be exposed to the receiving space of the slot 121, and then the biological sample to be analyzed (not shown) can be loaded on the chip 1.
  • the slot 121 is substantially aligned with the chip positioning area 111 in the thickness direction Z, so that the chip 1 loaded with the biological sample to be analyzed is at least partially received in the receiving space of the slot 121 or exposed to the reaction liquid added in the receiving space of the slot 121, and then, for example, various operations or biochemical reactions can be performed on the biological sample on the chip 1 in an analytical instrument (not shown).
  • the storage space of the biochip container is defined by the side walls of the groove 121 of the container body 120 and the surface of the glass slide 110.
  • various reaction liquids, waste liquids or products can also be accommodated in the storage space, such as reaction reagents or cleaning reagents that will undergo biochemical reactions with the biological sample on the chip 1, reaction waste liquids, or target products captured by the chip from the biological sample.
  • the slot 121 may penetrate the container body 120 in the thickness direction Z, and the container body 120 may be assembled on the surface of the glass slide 110 in a fluid-tight manner, for example, the container body 120 may be attached to the glass slide 110 by an adhesive or glue that does not affect the subsequent biochemical reaction on the chip, or by fasteners such as clamps and bolts, so that the container body 120 and the glass slide 110 are fluid-tight, so as to avoid or reduce the loss of various reaction liquids added to the containing space for biochemical reactions.
  • the container body may be flat, for example, it may be made of flat glass or rubber material.
  • the size or area of the slot 121 formed in the container body 120 is larger than the size or area of the chip 1 to be processed, and the slot 121 may have a shape that roughly matches or is similar to the shape of the chip, so that the chip and the biological sample loaded thereon can be accommodated in the defined accommodation space.
  • a plurality of slots may be formed in the container body, and each slot may have the same or different sizes to achieve multi-chip, high-throughput processing.
  • the accommodation space of the biochip container may be configured to accommodate, for example, chips with a size ranging from 1 cm to 13 cm, including chips of standard and non-standard sizes, so as to be compatible with or adapt to a variety of different applications.
  • the wall thickness D of the slot 121 is greater than the thickness d of the chip 1, and the wall thickness D of the slot 121 may be designed according to specific needs to ensure that a liquid layer with a desired thickness can be maintained above the chip and the biological sample carried thereon during the biochemical reaction.
  • the cover glass 130 is disposed on the surface of the container body 120 facing away from the slide glass 110.
  • the cover glass 130 can be assembled to the container body 120 using an adhesive or glue that does not affect the subsequent biochemical reaction on the chip, or by fasteners such as clamps and bolts.
  • the container body 120 and the cover glass 130 can also be fluid-tight to avoid or reduce the loss of various reaction liquids added to the containing space for biochemical reactions. It can be understood that in some applications, it is not required that the container body and the cover glass must be fluid-tight, for example, the groove of the container body has a sufficient depth, the container is kept horizontal during operation, etc., to ensure that the reaction liquid does not enter the interface between the container body and the cover glass.
  • the cover glass 130 has at least a transparent portion 131. After the cover glass 130 is assembled to the container body 120, the transparent portion 131 is substantially aligned with the slot 121 of the container body 120 in the thickness direction Z and covers the slot 121 or the accommodation space defined by the slot 121, so as to allow the biological sample on the chip 1 accommodated in the accommodation space of the slot 121 to be photographed through the transparent portion 131. Exemplarily, such photography can be performed by placing the biochip container 100 containing the chip loaded with the biological sample in a bioanalysis instrument. Exemplarily, the cover glass is transparent as a whole or at least the portion covering the slot of the container body is transparent or made of a transparent material such as glass.
  • the size of the cover glass may be smaller than or equal to the size of the container body and/or the slide glass; the size of the slide glass may be smaller than, equal to, or larger than the size of the container body.
  • the cover glass 130 may be formed with a through hole, which may penetrate the cover glass 130 to be in fluid communication with the receiving space of the container body 120, so as to allow various operations such as liquid transfer and liquid replacement to be performed through the through hole, including adding reaction liquid to the receiving space, extracting waste liquid from the receiving space, and/or recovering reaction products from the receiving space, etc.
  • the through hole is formed in a portion of the cover glass 130 that does not overlap with the groove 121 in the thickness direction Z, such as formed outside the transparent portion 131, or formed in a portion of the cover glass 130 that is located outside the groove 121 in a direction perpendicular to the thickness direction Z, so as to avoid interfering with the photographing or imaging of the biological sample.
  • the through holes formed in the cover glass 130 include a first through hole 132 and a second through hole 133 located at different positions; for example, as shown in FIGS. 1-4 and 8-9, the first through hole 132 and the second through hole 133 are located on opposite sides of the slot 121 in a direction perpendicular to the thickness direction.
  • the reaction liquid can be added to the storage space of the slot 121 through the first through hole 132 and/or the second through hole 133; the waste liquid can be first extracted from the storage space through the first through hole 132, and then the remaining waste liquid can be extracted from the storage space through the second through hole 133, and vice versa; similarly, the reaction product can be first recovered from the storage space through the first through hole 132, and then the remaining reaction product can be recovered from the storage space through the second through hole 133, and vice versa.
  • the first through hole 132 and the second through hole 133 can have the same opening shape, or have different opening shapes (for example, as shown in Figures 1-3 and 8), so as to facilitate different operations at different through holes.
  • the opening shape of the through hole can be appropriately designed to meet the requirements for performing these operations, such as facilitating the pipetting and liquid replacement operations of the pipette gun.
  • the through hole formed in the cover glass 130 may be directly connected to the receiving space of the groove 121, or may be indirectly connected (such as through a conduit) to the receiving space of the groove 121.
  • the container body 120 may also be formed with an opening, which is substantially aligned with the through hole in the cover glass 130 in the thickness direction Z and is in fluid communication with both the receiving space and the through hole, so that the above-mentioned various operations such as pipetting and liquid replacement can be performed through both the through hole in the cover glass 130 and the opening in the container body 120.
  • One or more openings may be formed in the container body 120, and the openings may be located at the periphery or edge of the slot 121.
  • the openings formed in the container body 120 include a first opening 122 and a second opening 123 located on different sides (such as opposite sides) of the slot 121, as shown in FIGS. 2-6 and 8-11.
  • the openings formed in the container body 120 may correspond one-to-one with the through holes formed in the cover glass 130 in position.
  • the first opening 122 is substantially aligned with the first through hole 132 in the thickness direction Z
  • the second opening 123 is substantially aligned with the second through hole 133 in the thickness direction Z.
  • the openings formed in the container body may be through holes that penetrate the container body in the thickness direction Z, or may be slots that only partially extend through the container body in the thickness direction Z.
  • a through hole is formed in the cover glass that is fluidly connected to the opening formed in the container body
  • the biochip container 100 includes a glass slide 110, a container body 120 and a cover glass 130
  • a biochip container that does not include a glass slide or a cover glass can be provided or used, which can also accommodate chips of non-standard sizes and ensure that automated operations such as imaging, liquid replacement, and transfer are performed on the chip on the analytical instrument without damaging the chip.
  • a biochip container 100 ′ which includes a stacked container body 120 and a cover glass 130, without the above-mentioned slide glass, or the slide glass is formed integrally with the container body.
  • the groove 121 formed in the container body 120 is a bottomed groove, which is open on the side facing the cover glass 130, and the bottomed groove defines a receiving space suitable for receiving the chip 1.
  • the cover glass 130 is assembled to the container body 120 to cover the groove 121, and then the biochip container can be transferred or operated, and the biological sample in the groove 121 is imaged through the transparent part 131 of the cover glass 130, and the operations such as liquid transfer, liquid replacement, product recovery, etc. are performed through the through holes 132, 133 in the cover glass 130 and/or the openings 122, 123 in the container body 120.
  • a biochip container 100′′ which includes a stacked glass slide 110 and a container body 120, without the above-mentioned cover glass, or the cover glass or the transparent part is formed as a single body with the container body or is an integral part of the container body.
  • the glass slide 110 has a chip positioning area on the surface of one side, and the chip 1 is suitable for being fixed in the chip positioning area.
  • the container body 120 is suitable for being sealed and assembled on the surface of the glass slide 110, and the groove 121 passes through the container body 120 in the thickness direction and is substantially aligned with the chip positioning area, so that the chip 1 and the biological sample loaded thereon are The container body 120 is exposed to the accommodation space defined by the groove 121.
  • the container body 120 has a transparent portion 124, which covers the groove 121 on the side opposite to the glass slide 110.
  • the transparent portion 124 may have a size greater than or equal to the opening size of the groove 121.
  • the provided biochip container itself includes a chip, which can be fixed on a glass slide or in a bottomed groove of a container body, and the biochip container can be assembled after the biological sample is loaded into the chip.
  • the exemplary embodiment of the present disclosure also provides a method for preparing a biochip container 100, which mainly includes the following steps:
  • the flat carrier as the container body 120 is processed to form a groove 121 therein, the groove defining a receiving space suitable for receiving the chip 1 on which the biological sample to be analyzed will be loaded;
  • the assembled cover glass 130 and the container body 120 are sealed and assembled to the glass slide 110, so that the chip 1 loaded with the biological sample to be analyzed is accommodated in the accommodating space.
  • a method for preparing a biochip container 100′ is also provided, which mainly includes the following steps:
  • the flat plate carrier as the container body 120 is processed to form a bottom groove 121 in the flat plate carrier, and the bottom groove 121 defines a receiving space suitable for receiving the chip 1 on which the biological sample to be analyzed will be loaded;
  • the cover glass 130 is assembled onto the container body 120 so that at least the transparent portion 131 of the cover glass 130 is covered with the bottom groove 121 .
  • a method for preparing a biochip container 100 ′′ is also provided, which mainly includes the following steps:
  • the flat plate carrier as the container body 120 is processed to form a bottom groove 121 in the flat plate carrier, the bottom groove defines a receiving space suitable for receiving the chip 1 on which the biological sample to be analyzed is loaded, and the bottom of the bottom groove is a transparent portion 124;
  • the container body 120 is sealedly assembled to the glass slide 110 on the side thereof facing away from the transparent part 124, so that the chip 1 loaded with the biological sample to be analyzed is accommodated in the accommodating space to allow the biological sample to be photographed through the transparent part 124.
  • a through hole can also be formed in the cover glass 130, and the through hole is connected to the fluid of the receiving space after the cover glass 130 is assembled on the container body 120, so as to allow one or more operations to be performed through the through hole, including adding reaction liquid to the receiving space, extracting waste liquid from the receiving space, recovering reaction products from the receiving space, etc.
  • the chip 1 can be fixed in the chip positioning area of the glass slide 110, so that the prepared biochip container itself includes a chip, and then the biological sample to be analyzed can be loaded onto the chip 1, and then the container body 120 and the glass slide 110 are sealed and assembled.
  • an opening can be formed in the flat carrier, and the opening is connected to the fluid in the receiving space to allow one or more operations to be performed alone through the opening, or through both the opening and the through hole, including adding reaction liquid to the receiving space, extracting waste liquid from the receiving space, recovering reaction products from the receiving space, etc.
  • the biochip container provided in the embodiments of the present disclosure can be used to analyze biological samples, wherein after the biological sample is loaded on the chip and the assembly of the biochip container is completed, the biochip container containing the chip carrying the biological sample can be clamped or adsorbed to place the biochip container in or remove it from an analytical instrument, which can include, for example, a spatial omics or spatiotemporal omics operation instrument; the biological sample in the biochip container can be photographed in the analytical instrument; in the analytical instrument, operations such as pipetting, waste liquid extraction, biochemical reactions (such as staining, in situ information labeling, capture, etc.), and product recovery can be performed in the biochip container.
  • an analytical instrument which can include, for example, a spatial omics or spatiotemporal omics operation instrument
  • the biological sample in the biochip container can be photographed in the analytical instrument
  • operations such as pipetting, waste liquid extraction, biochemical reactions (such as staining, in situ information labeling, capture, etc.), and product recovery can be performed in the biochip container.
  • a biochip container which provides a solution for the automation of chip photography and subsequent experimental operations.
  • the chip can be placed in the container by tools such as tweezers, and then various operations can be performed on the container, such as clamping or adsorption operations, placing the container containing the biochip in a spatial omics or spatiotemporal omics operation instrument, photographing the biological sample loaded on the chip, performing operations such as pipetting, waste liquid extraction, biochemical reactions, and product recovery in the container, thereby completing a series of biochemical operations including heating.
  • this chip container to accommodate the biochip can perform biochemical reactions such as staining, in-situ information labeling, and capture.
  • biochips of different sizes can be placed in the chip container, which is compatible with the pipetting, waste liquid extraction, biochemical reactions, product recovery and other operational processes of biochips of different sizes; in addition, multiple chip slots can be provided on the biochip container so that multiple full-chip samples can be processed simultaneously to achieve high-throughput processing.

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Abstract

La présente divulgation concerne un contenant de biopuce et des procédés de fabrication et d'utilisation de celui-ci. Le contenant de biopuce comprend un corps de contenant (120) pourvu d'une fente (121), la fente définissant un espace de logement, l'espace de logement étant conçu pour loger une puce (1) ; un échantillon biologique à analyser sera chargé sur la puce ; le contenant de biopuce comporte une partie transparente (131 ; 124), la partie transparente étant sensiblement alignée sur la fente (121) et la recouvrant dans le sens de l'épaisseur (z), de manière à permettre à l'échantillon biologique sur la puce logée dans l'espace de logement d'être photographié au travers de la partie transparente.
PCT/CN2022/127996 2022-10-27 2022-10-27 Contenant de biopuce et ses procédés de préparation et d'utilisation WO2024087107A1 (fr)

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