WO2024082134A1 - 一种高通量制备用于质谱检测的微生物样品的取样装置 - Google Patents

一种高通量制备用于质谱检测的微生物样品的取样装置 Download PDF

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Publication number
WO2024082134A1
WO2024082134A1 PCT/CN2022/125918 CN2022125918W WO2024082134A1 WO 2024082134 A1 WO2024082134 A1 WO 2024082134A1 CN 2022125918 W CN2022125918 W CN 2022125918W WO 2024082134 A1 WO2024082134 A1 WO 2024082134A1
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sampling
plate body
mounting seat
needles
groove
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PCT/CN2022/125918
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English (en)
French (fr)
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司同
付立豪
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中国科学院深圳先进技术研究院
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Priority to PCT/CN2022/125918 priority Critical patent/WO2024082134A1/zh
Publication of WO2024082134A1 publication Critical patent/WO2024082134A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler

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  • the present application relates to the technical field of microbial detection, and in particular to a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection.
  • the purpose of the present application is to provide a high-throughput sampling device for preparing microbial samples for mass spectrometry detection, so as to solve the problems of complex microbial sampling operation and low efficiency in the prior art.
  • An embodiment of the present application provides a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection, comprising: a mounting seat; a plurality of sampling needles, which are arranged on the mounting seat in a preset arrangement and at intervals, and each of the sampling needles comprises: an elongated body roughly perpendicular to the mounting seat; a connecting end formed at one end of the body and connected to the mounting seat; and a sampling end formed at the other end of the body.
  • a plurality of sampling needles are provided on the mounting seat, and a plurality of microbial samples can be obtained through a single sampling operation, thereby simplifying the sampling operation and improving the sampling efficiency.
  • FIG1 is a schematic diagram of a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection according to an embodiment of the present application
  • FIG2 is a side view of the sampling device in FIG1 ;
  • FIG3 is a perspective view of the sampling device in FIG1 ;
  • FIG4 is a top view of the sampling device in FIG1 ;
  • FIG5 is a schematic diagram of a sampling needle according to an embodiment of the present application.
  • FIG6 is a schematic diagram of a first plate body according to an embodiment of the present application.
  • FIG7 is a schematic diagram of a second plate body according to an embodiment of the present application.
  • FIG8 is a schematic diagram of a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection according to another embodiment of the present application.
  • FIG9 is a side view of the sampling device in FIG8 ;
  • FIG10 is a schematic diagram of a bracket according to an embodiment of the present application.
  • FIG. 11 is a side view of the bracket in FIG. 10 .
  • the embodiment of the present application provides a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection, which is used for sampling microorganisms.
  • a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection can be used for sampling during MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) detection.
  • MALDI-TOF-MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • MALDI-TOF-MS is a new type of high-throughput mass spectrometry. Its principle is to use laser to irradiate the co-crystallized film formed by the sample and the matrix. The matrix absorbs energy from the laser and transfers it to the biomolecules. During the ionization process, protons are transferred to the biomolecules or protons are obtained from the biomolecules, thereby ionizing the biomolecules.
  • MALDI-TOF-MS can directly detect microbial clones without the need for complex sample extraction processes and large-scale cultivation.
  • the sampling device of the present application can be used to transfer microbial clones from the culture plate to the detection metal target plate to replace manual sampling.
  • FIGS. 1 to 4 are schematic diagrams of a sampling device for high-throughput preparation of microbial samples for mass spectrometry detection according to an embodiment of the present application.
  • the sampling device includes a mounting seat 1 and a plurality of sampling needles 2.
  • the plurality of sampling needles 2 are arranged on the mounting seat 1 at intervals in a preset arrangement.
  • the mounting seat 1 is generally plate-shaped, and the plurality of sampling needles 2 are arranged on one side of the mounting seat 1 in a plurality of rows and columns, each sampling needle 2 is roughly perpendicular to the mounting seat 1, and the plurality of sampling needles 2 are roughly parallel.
  • the number of sampling needles 2 is 96, and the 96 sampling needles 2 are arranged in 12 rows and 8 columns.
  • the number of sampling needles 2 and the arrangement of the sampling needles 2 can be set according to actual sampling requirements, and this application does not impose any restrictions on this.
  • each sampling needle 2 comprises a main body 21, a connecting end 22 and a sampling end 23.
  • the main body 21 is an elongated structure and is substantially perpendicular to the mounting base 1.
  • the connecting end 22 is formed at one end of the main body 21 and is connected to the mounting base 1.
  • the sampling end 23 is formed at the other end of the main body 21 and is used for sampling.
  • the grasping mechanism can be used to grasp the mounting base 1 and move it to the location to be sampled. Multiple microbial samples can be simultaneously obtained from the location to be sampled through multiple sampling needles 2, thereby achieving multiple microbial samples at one time, simplifying the sampling operation, and improving sampling efficiency.
  • the sampling needle 2 is detachably connected to the mounting base 1 via the connecting end 22 to facilitate replacement and maintenance of the sampling needle 2 and sterilization of the entire sampling needle 2 .
  • the mounting seat 1 includes a first plate body 11 and a second plate body 12 detachably connected in a stacked state.
  • the first plate body 11 and the second plate body 12 may be stainless steel plates.
  • a plurality of through holes 111 for a plurality of sampling needles 2 to pass through are provided on the first body, the main body 21 of the sampling needle 2 passes through the through holes 111 of the first plate body 11, and the connecting end 22 of the sampling needle 2 is clamped by the first plate body 11 and the second plate body 12 in a stacked state to fix the sampling needle 2 on the mounting seat 1.
  • the sampling needle 2 can be easily removed from the mounting seat 1.
  • the first plate body 11 and the second plate body 12 can be detachably connected by a screw 13.
  • a plurality of receiving grooves 112 are provided on the first plate body 11.
  • the receiving grooves 112 are part of the through hole 111 for the sampling needle 2 to pass through and can accommodate a part of the connecting end 22 of a single sampling needle 2.
  • a groove 121 is provided on the second plate body 12 for accommodating another part of the connecting ends 22 of the plurality of sampling needles 2. As shown in FIG.
  • the groove 121 and the plurality of receiving grooves 112 are connected to jointly accommodate the connecting ends 22 of the plurality of sampling needles 2, so as to achieve clamping of the connecting ends 22 of the plurality of sampling needles 2.
  • the main body 21 of the sampling needle 2 is passed through the through hole 111 of the first plate body 11 until a part of the connecting end 22 of the sampling needle 2 is located in the receiving groove 112, and the other part of the connecting end 22 protrudes from the first plate body 11;
  • the second plate 12 is placed on the first plate 11 with the groove 121 facing the first plate 11. At this time, another part of the connecting end 22 is accommodated by the groove 121, and the end surface of the connecting end 22 away from the groove 121 contacts the bottom surface of the groove 121.
  • first plate body 11 and the second plate body 12 are connected to clamp and fix the connecting end 22 .
  • each accommodating groove 112 is a conical groove, which is used to accommodate the smaller diameter portion of a single conical head.
  • the groove 121 is a square groove, which is used to accommodate the larger diameter portions of multiple conical heads.
  • the mounting seat 1 and the connecting end 22 of the sampling needle 2 can be detachably connected by a threaded connection.
  • This method requires that the multiple sampling needles 2 be screwed onto the mounting seat 1 one by one, while the previous solution only requires that the multiple sampling needles 2 be inserted into the multiple through holes 111 of the first plate body 11 one by one, so the operation of the previous solution is simpler and faster.
  • the sampling needle 2 further includes an elastic sleeve 24, which is removably sleeved on the outside of the other end of the main body 21 to form a sampling end 23.
  • the sampling end 23 dips the microbial sample through the elastic sleeve 24 to achieve sampling.
  • the elastic sleeve 24 can be removed from the other end of the main body 21 for disinfection.
  • the elastic sleeve 24 is sleeved on the outside of the other end of the main body 21, which is very convenient to operate.
  • One end of the elastic sleeve 24 is open and the other end is closed.
  • the closed end of the elastic sleeve 24 can cover the end surface of the other end of the main body 21, which is the main sampling part.
  • the elastic sleeve 24 has a certain elasticity, and the inner diameter of the elastic sleeve 24 can be slightly smaller than the outer diameter of the other end of the main body 21, so the elastic sleeve 24 can be tightly covered on the other end of the main body 21 and is not easy to fall off.
  • the elastic sleeve 24 is a silicone sleeve.
  • the main body 21 and the conical head are made of stainless steel, so that the sampling needle 2 has better rigidity.
  • the sampling device further includes a support 3, and the support 3 is used to support the mounting seat 1 in a manner that the multiple sampling needles 2 are facing downward and suspended.
  • the mounting seat 1 is placed on the support 3 with the sampling needles 2 facing downward; when sampling is required, the grabbing mechanism can grab the mounting seat 1 on the support 3; after sampling, the grabbing mechanism can put the mounting seat 1 back on the support 3.
  • the bracket 3 includes a support platform 31 and a positioning structure 32.
  • the support platform 31 is used to support the outer peripheral portion of the mounting seat 1, and the space 33 inside the support platform 31 is used to accommodate a plurality of sampling needles 2.
  • the positioning structure 32 is provided on the outer peripheral side of the support platform 31, and is used to position the mounting seat 1 on the support platform 31 to prevent the mounting seat 1 from being displaced relative to the support platform 31.
  • the positioning structure 32 includes a first boss 321 provided on opposite sides of the length direction of the support platform 31, and a second boss 322 provided on opposite sides of the width direction of the support platform 31.
  • the first boss 321 may be a block-shaped structure, and the number of the first boss 321 may be four, that is, two first bosses 321 are provided on opposite sides of the length direction of the support platform 31, and the two first bosses 321 on the same side are arranged at intervals along the width direction of the support platform 31.
  • the second boss 322 may be a strip-shaped structure, and the number of the second boss 322 may be two, that is, one second boss 322 is provided on opposite sides of the width direction of the support platform 31, and each second boss 322 extends along the length direction of the support platform 31.
  • an inclined surface 323 is provided on the inner side of the second boss 322 facing the support platform 31, which is used to guide the mounting seat 1 when the mounting seat 1 is placed, so that the mounting seat 1 falls onto the support platform 31 along the inclined surface 323.
  • the bracket 3 further includes four supporting legs, and the supporting platform 31 is supported by the four supporting legs.
  • two opposite sides of the mounting seat 1 are respectively provided with protruding structures 14 for cooperating with the grabbing mechanism.
  • each of the two opposite sides of the mounting seat 1 is provided with a plurality of spaced protruding structures 14, and the protruding structures 14 protrude outward along the plane where the mounting seat 1 is located.
  • the protruding structures 14 are provided on two opposite sides of the second plate body 12.
  • the adapter head of the 96-channel pipette is used as a grasping mechanism to grasp the mounting seat 1 placed on the bracket 3 on the table of the automated pipetting workstation and move it to the sampling location, and the adapter head of the pipette descends to dip the 96 dot matrix colonies through multiple sampling needles 2 to obtain bacterial samples of the 96 dot matrix colonies; then the adapter head of the pipette continues to drive the mounting seat 1 to move to the metal target plate for mass spectrometry detection, and prints the dipped bacterial sample on the metal target plate; then the adapter head of the pipette continues to drive the mounting seat 1 to move to the bracket 3, and puts the mounting seat 1 back on the support platform 31 of the bracket 3;
  • the experimenter can add organic reagents to the metal target plate for MALDI-TOF-MS detection and analysis.
  • 96 microbial samples can be sampled at one time, and the sampling operation can be completed within 1 minute. Compared with the manual method which takes about 60 minutes to complete the sampling of 96 microbial samples, the sampling efficiency is greatly improved.

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Abstract

一种高通量制备用于质谱检测的微生物样品的取样装置，包括：安装座；多个取样针，以预设排列方式间隔设置于安装座上，各取样针分别包括：大致垂直于安装座的细长的主体；形成于主体的一端且与安装座连接的连接端；形成于主体的另一端的取样端。本申请的取样装置通过一次取样操作即可获取多个微生物样品，简化取样操作，提高取样效率。

Description

一种高通量制备用于质谱检测的微生物样品的取样装置 技术领域
本申请涉及微生物检测技术领域，尤其是一种高通量制备用于质谱检测的微生物样品的取样装置。
背景技术
在微生物检测中，经常需要对微生物取样。
但目前通常采用手工取样方法对微生物取样，操作复杂，取样效率低。
发明内容
本申请的目的是提供一种高通量制备用于质谱检测的微生物样品的取样装置，以解决现有技术中微生物取样操作复杂、效率低的问题。
本申请实施例提供一种高通量制备用于质谱检测的微生物样品的取样装置，包括：安装座；多个取样针，以预设排列方式间隔设置于所述安装座上，各所述取样针分别包括：大致垂直于所述安装座的细长的主体；形成于所述主体的一端且与所述安装座连接的连接端；形成于所述主体的另一端的取样端。
本申请实施例的取样装置中，安装座上设有多个取样针，通过一次取样操作即可获取多个微生物样品，简化取样操作，提高取样效率。
附图说明
为了更清楚地说明本说明书实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本说明书中记载的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。在附图中：
图1是本申请一实施例的高通量制备用于质谱检测的微生物样品的取样装置的示意图；
图2是图1中取样装置的侧视图；
图3是图1中取样装置的透视图；
图4是图1中取样装置的俯视图；
图5是本申请一实施例的取样针的示意图；
图6是本申请一实施例的第一板体的示意图；
图7是本申请一实施例的第二板体的示意图；
图8是本申请另一实施例的高通量制备用于质谱检测的微生物样品的取样装置的示意图；
图9是图8中取样装置的侧视图；
图10是本申请一实施例的支架的示意图；
图11是图10中支架的侧视图。
具体实施方式
为了使本技术领域的人员更好地理解本说明书中的技术方案，下面将结合本说明书实施例中的附图，对本说明书实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本说明书一部分实施例，而不是全部的实施例。基于本说明书中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都应当属于本说明书保护的范围。
下面参照附图对本申请实施例的实施方式进行说明。
本申请实施例提供一种高通量制备用于质谱检测的微生物样品的取样装置，用于对微生物取样。
例如，高通量制备用于质谱检测的微生物样品的取样装置可以用于MALDI-TOF-MS(基质辅助激光解析电离飞行时间质谱)检测时的取样。MALDI-TOF-MS是一种新型高通量质谱，其原理是用激光照射样品与基质形成的共结晶薄膜，基质从激光中吸收能量传递给生物分子，而电离过程中将质子转移到生物分子或从生物分子得到质子，而使生物分子电离的过程。其相对于高效液相色谱串联质谱的较长检测时间，仅需5～10秒即可完成一个样本的检测。并且MALDI-TOF-MS可直接对微生物克隆进行检测，不需要复杂的样品提取过程和大量培养。在进行MALDI-TOF-MS检测之前，可以采用本申请的取样装置将微生物克隆从培养平板中转移到检测金属靶板上，以替代手工取样。
图1至图4是本申请一实施例的高通量制备用于质谱检测的微生物样品的取样装置的示意图。
如图1至图4所示，取样装置包括安装座1和多个取样针2。多个取样针2以预设排列方式间隔设置于安装座1上。在图1至图4的示例中，安装座1大体呈板状，多个取样针2以多行多列的排列方式设置于安装座1的一侧，各取样针2大致垂直于安装座1，多个取样针2大致平行。例如，取样针2的数量为96个，96个取样针2排列为12行8列。当然，取样针2的数量、以及取样针2的排列方式都可以根据实际取样需求进行设置，本申请对此不 作限制。
如图1-图3和图5所示，各取样针2分别包括主体21、连接端22和取样端23。主体21为细长的结构，且大致垂直于安装座1。连接端22形成于主体21的一端并与安装座1连接。取样端23形成于主体21的另一端，用于取样。
采用本实施例的取样装置取样时，可以利用抓取机构抓取安装座1并移动至待取样处，通过多个取样针2可以同时从待取样处获取多个微生物样品，从而实现一次获取多个微生物样品，简化取样操作，提高取样效率。
在一些实施例中，取样针2通过连接端22与安装座1可拆卸地连接，以方便更换和维修取样针2、以及对取样针2整体进行消毒。
在本实施例的一种可行技术方案中，如图1、图3所示，安装座1包括以叠置状态可拆卸地连接的第一板体11和第二板体12。第一板体11和第二板体12可以是不锈钢板。如图3、图6所示，第一本体上设有多个供多个取样针2穿过的穿孔111，取样针2的主体21穿过第一板体11的穿孔111，且取样针2的连接端22由处于叠置状态的第一板体11和第二板体12夹持，以将取样针2固定于安装座1上。由于第一板体11和第二板体12以夹持方式固定取样针2的连接端22，在需要更换、维修取样针2，或需要对取样针2整体进行消毒时，可以很方便地将取样针2从安装座1上拆下。例如，第一板体11和第二板体12可以通过螺钉13可拆卸地连接。
在图3和图6的示例中，第一板体11上设有多个容纳槽112，容纳槽112作为穿孔111的一部分，供取样针2穿过且能容纳单个取样针2的连接端22的一部分。在图3和图7的示例中，第二板体12上设有一个凹槽121，用于容纳多个取样针2的连接端22的另一部分。如图3所示，在第一板体11和第二板体12处于叠置状态时，凹槽121和多个容纳槽112连通，以共同容纳多个取样针2的连接端22，以实现对多个取样针2的连接端22的夹持。
将取样针2安装于安装板的操作如下：
将取样针2的主体21穿过第一板体11的穿孔111，直至取样针2的连接端22的一部分位于容纳槽112内，此时连接端22的另一部分凸出于第一板体11；
再将第二板体12以凹槽121朝向第一板体11的状态放置于第一板体11上，此时连接端22的另一部分被凹槽121容纳，且连接端22的远离的端面与凹槽121的底面相接触；
然后将第一板体11和第二板体12连接，从而将连接端22夹持固定。
将取样针2从安装板拆卸的操作与上述安装操作流程相反，故不赘述。
在图5的示例中，取样针2的连接端22为锥形头，锥形头具有直径较小部分和直径较大部分，锥形头的直径较小部分与主体21连接。在图6的示例中，各容纳槽112为锥形槽，用 于容纳单个锥形头的直径较小部分。在图7的示例中，凹槽121为方形槽，用于容纳多个锥形头的直径较大部分。参见图3，当取样针2的主体21穿过第一板体11的穿孔111时，取样针2的锥形头的直径较小部分与锥形槽相配合，这种锥形面配合的结构，不仅在取样针2的径向方向上对其定位，并且允许第二板体12在取样针2的轴向方向上对其压紧和定位，从而将取样针2稳固地固定在安装板上。
在本实施例的另一种可行技术方案中，安装座1和取样针2的连接端22可以通过螺纹连接方式可拆卸地连接。此方式需要将多个取样针2逐个旋紧于安装座1上，而前一种方案只需要将多个取样针2逐个插在第一板体11的多个穿孔111内，因此前一种方案的操作更简便、快捷。
在一些实施例中，如图5所示，取样针2还包括弹性套24，弹性套24可移除地套于主体21的另一端外侧，以形成取样端23。取样端23通过弹性套24蘸取微生物样品，实现取样。取样结束后，可以将弹性套24从主体21的另一端取下进行消毒，消毒完毕后，将弹性套24套在主体21的另一端外侧，操作非常方便。
弹性套24的一端敞口、另一端封闭，弹性套24的封闭端能够包覆主体21的另一端的端面，是主要取样部位。弹性套24具有一定弹性，弹性套24的内径可以略小于主体21另一端的外径，因此弹性套24可以紧套在主体21的另一端而不易掉落。
可选地，弹性套24为硅胶套。
可选地，主体21和锥形头的材质为不锈钢，以使取样针2具有较好的刚性。
在一些实施例中，如图8、图9所示，取样装置还支架3，支架3用于对安装座1以多个取样针2朝下且悬空的方式进行支撑。在不需要取样时，将安装座1以取样针2朝下的方式放置在支架3上；当需要取样时，抓取机构可以抓取支架3上的安装座1；取样结束后，抓取机构可以将安装座1再放回支架3上。
在一种可行的技术方案中，如图10所示，支架3包括支撑平台31和定位结构32。支撑平台31用于支撑安装座1的外周部分，支撑平台31内侧的空间33用于容置多个取样针2。定位结构32设于支撑平台31的外周侧，用于将安装座1定位于支撑平台31，以阻止安装座1相对于支撑平台31的错位移动。
在图10和图11的示例中，定位结构32包括设于支撑平台31的长度方向的相对两侧的第一凸台321、以及设于支撑平台31的宽度方向的相对两侧的第二凸台322。第一凸台321可以是方块形结构，第一凸台321的数量可以是四个，即，支撑平台31的长度方向的相对两侧分别设有两个第一凸台321，位于同一侧的两个第一凸台321沿支撑平台31的宽度方向间隔设置。第二凸台322可以是长条形结构，第二凸台322的数量可以是两个，即，支撑平台 31的宽度方向的相对两侧分别设有一个第二凸台322，每个第二凸台322沿支撑平台31的长度方向延伸。可选地，第二凸台322的朝向支撑平台31的内侧设有倾斜面323，用于在放置安装座1时对安装座1导向，使安装座1沿该倾斜面323下落至支撑平台31上。
如图10所示，支架3还包括四条支撑腿，支撑平台31由四条支撑腿支撑。
在一些实施例中，如图1所示，安装座1的两个相对的侧边分别设有用于与抓取机构配合的凸出结构14。例如，安装座1的两个相对的侧边的每一个设有多个间隔的凸出结构14，凸出结构14沿安装座1所在的平面朝外凸出。具体是，例如，凸出结构14设于第二板体12的两个相对的侧边。
以下结合一应用实例说明本申请的取样装置的操作方法。
在需要进行MALDI-TOF-MS(基质辅助激光解析电离飞行时间质谱)检测时，通常需要首先培养菌落，方法是，采用自动化移液工作站的移液器(例如96通道移液器)将微生物菌液滴加到在方形平皿上，培养后形成96点阵的菌落；
然后，以96通道移液器的适配头作为抓取机构，对放置在自动化移液工作站台面上的支架3上的安装座1进行抓取并移动至取样处，移液器的适配头下降，以通过多个取样针2对96点阵菌落进行蘸取，获取96点阵菌落的菌体样品；然后移液器的适配头继续带动安装座1移动至质谱检测的金属靶板处，并将蘸取的菌体样品拓印到金属靶板上；之后移液器的适配头继续带动安装座1移动至支架3处，并将安装座1放回到支架3的支撑平台31上；
之后，实验人员可以在金属靶板上添加有机试剂，以进行MALDI-TOF-MS的检测分析。
使用本申请的取样装置，可以一次完成96个微生物样品的取样，1min内即可完成取样操作，相较于手工方法需要60min左右才能完成96个微生物样品的取样，大幅提高取样效率。
以上所述仅为本说明书的实施例而已，并不用于限制本说明书。对于本领域技术人员来说，本说明书可以有各种更改和变化。凡在本说明书的精神和原理之内所作的任何修改、等同替换、改进等，均应包含在本说明书的权利要求范围之内。

Claims (10)

  1. 一种高通量制备用于质谱检测的微生物样品的取样装置，其中，包括：
    安装座；
    多个取样针，以预设排列方式间隔设置于所述安装座上，各所述取样针分别包括：
    大致垂直于所述安装座的细长的主体；
    形成于所述主体的一端且与所述安装座连接的连接端；
    形成于所述主体的另一端的取样端。
  2. 根据权利要求1所述的装置，其中，
    所述取样针通过所述连接端与所述安装座可拆卸地连接。
  3. 根据权利要求2所述的装置，其中，
    所述安装座包括以叠置状态可拆卸地连接的第一板体和第二板体；
    所述取样针的主体穿过所述第一板体，且所述取样针的连接端由处于所述叠置状态的所述第一板体和所述第二板体夹持。
  4. 根据权利要求3所述的装置，其中，
    所述第一板体上设有多个容纳槽，分别用于容纳多个所述取样针的连接端的一部分；所述第二板体上设有一个凹槽，用于容纳多个所述取样针的连接端的另一部分；
    在所述第一板体和所述第二板体处于所述叠置状态时，所述凹槽和多个所述容纳槽连通，以共同容纳多个所述取样针的连接端。
  5. 根据权利要求4所述的装置，其中，
    所述取样针的连接端为锥形头；
    各所述容纳槽为锥形槽，用于容纳单个所述锥形头的直径较小部分；
    所述凹槽为方形槽，用于容纳多个所述锥形头的直径较大部分。
  6. 根据权利要求3所述的装置，其中，
    所述取样针的连接端为锥形头；
    所述第一板体上设有朝向所述第二板体的锥形槽，用于与所述锥形头配合；
    在所述第一板体和所述第二板体处于所述叠置状态时，所述第二板体将所述锥形头压紧 于所述锥形头内。
  7. 根据权利要求1所述的装置，其中，所述取样针还包括：
    弹性套，可移除地套于所述主体的另一端外侧，以形成所述取样端。
  8. 根据权利要求7所述的装置，其中，所述弹性套为硅胶套。
  9. 根据权利要求1所述的装置，其中，还包括：
    支架，用于对所述安装座以多个所述取样针朝下且悬空的方式进行支撑。
  10. 根据权利要求9所述的装置，其中，所述支架包括：
    支撑平台，用于支撑所述安装座的外周部分，所述支撑平台内侧的空间用于容置多个所述取样针；
    定位结构，设于所述支撑平台的外周侧，用于将所述安装座定位于所述支撑平台上。
PCT/CN2022/125918 2022-10-18 2022-10-18 一种高通量制备用于质谱检测的微生物样品的取样装置 WO2024082134A1 (zh)

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* Cited by examiner, † Cited by third party
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JP2000146775A (ja) * 1998-11-04 2000-05-26 Shimadzu Corp 自動試料注入装置
CN105176865A (zh) * 2015-08-17 2015-12-23 湖北工业大学 亚硝酸菌的高通量筛选方法
CN206408226U (zh) * 2016-12-07 2017-08-15 常州英德生物科技有限公司 一种多孔板移液器
CN112725164A (zh) * 2020-12-14 2021-04-30 北京市理化分析测试中心 一种固体表面微生物采样器
CN114437910A (zh) * 2022-01-24 2022-05-06 内蒙古自治区农牧业科学院 一种快速复制转移培养微生物用手持式多针板
CN216837911U (zh) * 2022-02-28 2022-06-28 深圳先进技术研究院 微生物分离纯化装置

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000146775A (ja) * 1998-11-04 2000-05-26 Shimadzu Corp 自動試料注入装置
CN105176865A (zh) * 2015-08-17 2015-12-23 湖北工业大学 亚硝酸菌的高通量筛选方法
CN206408226U (zh) * 2016-12-07 2017-08-15 常州英德生物科技有限公司 一种多孔板移液器
CN112725164A (zh) * 2020-12-14 2021-04-30 北京市理化分析测试中心 一种固体表面微生物采样器
CN114437910A (zh) * 2022-01-24 2022-05-06 内蒙古自治区农牧业科学院 一种快速复制转移培养微生物用手持式多针板
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