WO2024034948A1 - Composition and method for preventing or treating scuticociliatosis in paralichthys olivaceus - Google Patents
Composition and method for preventing or treating scuticociliatosis in paralichthys olivaceus Download PDFInfo
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- WO2024034948A1 WO2024034948A1 PCT/KR2023/011121 KR2023011121W WO2024034948A1 WO 2024034948 A1 WO2024034948 A1 WO 2024034948A1 KR 2023011121 W KR2023011121 W KR 2023011121W WO 2024034948 A1 WO2024034948 A1 WO 2024034948A1
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- composition
- preventing
- scutica
- infection
- liquid sulfur
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- A61P33/04—Amoebicides
Definitions
- the present invention relates to a composition and method for preventing or treating scutica infection in flounder. More specifically, a composition for preventing or treating scutica infection in flatfish containing micronized liquid sulfur as an active ingredient, or a feed composition for preventing or improving flatfish, comprising the step of administering the composition directly to flatfish or to a farm. It relates to a method of preventing or treating scutica infection.
- Scuticosis a parasitic infectious disease
- Scuticociliatids a type of protozoan ciliary worm. It occurs when the parasite Scootica invades the subcutaneous tissue of flatfish and causes ulcers. If the fish body is infected with this worm, it continues to proliferate in the seedlings, increasing the mortality rate.
- Formalin is made by dissolving formaldehyde, a representative carcinogen, in water to a concentration of 35-38%. It is widely used as a disinfectant and preservative, and in Korea, it is classified as a poison and requires strict management under the Water Quality Conservation Act (Article 29), the Toxic Chemicals Control Act (Article 15), and the Waste Management Act (Article 25). Formaldehyde is classified as a hazardous substance according to the Clean Air Conservation Act (Article 2).
- Sulfur is used industrially for a variety of purposes, including manufacturing sulfuric acid, smelting nickel, vulcanizing rubber, and as a raw material for gunpowder, matches, and medicine.
- Sulfur has a melting point of about 118°C, and the H 2 S component extracted from the desulfurization process that removes sulfur content from petroleum products passes through a sulfur recovery facility and is produced as molten sulfur.
- Molten sulfur is transported in a state maintained above 132°C in well-equipped tanks, or cooled and pulverized to produce sulfur powder and distributed.
- the sulfur powder produced at this time typically has a maximum particle size of 127 ⁇ m or more.
- the purpose of the present invention is to provide a composition for preventing or treating scutica infection in flatfish containing micronized liquid sulfur as an active ingredient.
- Another object of the present invention is to provide a feed composition for preventing or improving scutica infection in flatfish containing micronized liquid sulfur as a feed additive.
- Another object of the present invention is to provide a method for preventing or treating scutica infection in flounder, comprising administering to a fish farm a composition containing micronized liquid sulfur as an active ingredient.
- Another object of the present invention is to provide a method for preventing or treating scutica infection in flatfish, comprising feeding the feed composition to flatfish.
- the present invention provides a composition for preventing or treating scutica infection in flounder, comprising micronized liquid sulfur as an active ingredient.
- micronized liquid sulfur refers to sulfur whose particle size is micronized.
- the maximum particle size of sulfur is 10 to 60 ⁇ m, preferably 10 to 20 ⁇ m, and the average particle size of sulfur is 10 ⁇ m or less. Preferably it is 5 ⁇ m or less.
- the micronized liquid sulfur is prepared by mixing 5 to 50% by weight of sulfur powder, 0.01 to 0.05% by weight of surfactant, and the remaining water and grinding in a wet mill for more than 5 hours, preferably for 22 to 100 hours. It can be.
- the surfactant serves to evenly disperse the sulfur powder in water, and surfactants used in food, cosmetics, or veterinary medicine can be used.
- surfactants include alkyldimethylamine oxide, polysorbate (polysorbate 60, polysorbate 80), sorbitan (POE), POE (polyoxyethylene), and PEG (polyethylene glycol) (PEG 80, PEG).
- AOS Alpha Olefin Sulphonate
- AE Polyoxyethylene alkyl ether
- SLS Sodium lauryl sulfate
- SLES Sodium laureth sulfate
- tristyrylphenyl ether phosphate polyoxyethylene-polyoxypropylene copolymer lecithin (Lecithin) ), COCO-BETAIN, Solubilizer, Apple Wash, Emulsifying Wax, Olive Emulsifying Wax, Olive Oil peg-7 ESTERS ), Babassuamidopropyl Betain, Conacopa (Decyl Glucoside), triacetin, Calcium stearoyl lactate, Disodium Cocoamphodiacetate and mixtures thereof. You can.
- tristyrylphenyl etherphosphate was used as a surfactant (Preparation Example 1).
- Scootica is a ciliate parasite that parasitizes farmed fish and causes great damage to flounder fry being farmed.
- Scootica causes flounder scotiosis, which is caused by 30-40 ⁇ m Scootica ciliates and is used in marine fish cultured in aquaculture tanks on land or in offshore cages, especially artificial seedlings and breeding fish of flatfish. It causes deep infection in the body surface, fins, liver, blood vessels, and brain tissue, and causes mass death of flounder mostly through destruction of plastic connective tissue.
- this scutica ciliate is a disease that is difficult to treat when infected because it invades brain tissue through blood vessels and becomes parasitic.
- composition according to the present invention is suitable for preventing and treating scutica infection in flounder.
- prevention refers to any action that suppresses or delays the onset of scutica infection in flatfish by the composition
- treatment refers to any action that improves or beneficially changes the symptoms caused by scutica infection in flatfish by the composition. means action.
- the composition can be formulated and used in various forms according to conventional methods, and since it is used on fish, it may be most preferable to provide it in the form of a medicated bath.
- each formulation may be prepared by additionally including veterinary acceptable carriers such as buffers, preservatives, analgesics, solubilizers, isotonic agents, stabilizers, bases, excipients, lubricants, etc. known in the art. You can.
- veterinary acceptable carriers such as buffers, preservatives, analgesics, solubilizers, isotonic agents, stabilizers, bases, excipients, lubricants, etc. known in the art. You can.
- composition according to the present invention is administered in a veterinary effective amount.
- a veterinary effective amount refers to an amount that is sufficient to treat a disease with a reasonable benefit/risk ratio applicable to veterinary treatment and does not cause side effects.
- the effective dose level refers to the health status of the fish, severity, activity of the drug, It can be determined based on factors including sensitivity to the drug, method of administration, time of administration, route of administration and excretion rate, duration of treatment, drugs combined or used simultaneously, and other factors well known in the veterinary field.
- the composition when provided in the form of a medicated bath, it can be administered to the fish farm (water tank) at 100 to 1,000 ppm, preferably at 150 to 500 ppm.
- the bathing time of the medicinal bath for flatfish can be 1 to 4 hours, and the number of bathing times can be 1 time/day.
- the interval between drug baths may be 2 to 3 days.
- a feed composition for preventing or improving scutica infection in flatfish containing micronized liquid sulfur as a feed additive is provided.
- micronized liquid sulfur may be included in an amount of 0.01 to 3% by weight, preferably 0.01 to 1% by weight.
- the micronized liquid sulfur is less than the above range, the insecticidal efficacy against scutica insects is weak, and when it exceeds the above range, the toxicity of the substance itself may be a problem.
- Feed additives are a general term for substances added in small amounts to feed for nutritional or specific purposes. In the present invention, they refer to substances added for the purpose of preventing or improving scutica infection in fish.
- the micronized liquid sulfur of the present invention can be included in the feed composition as a feed additive.
- Feed refers to all foods supplied to fish.
- Formulated feeds commonly used in feed compositions include one or more protein sources selected from fish meal, casein, meat and bone meal, and shrimp powder, or one or more carbohydrate sources selected from sweet potato starch, potato starch, and wheat starch, or rapeseed oil, soybean oil, and corn.
- carbohydrate sources selected from sweet potato starch, potato starch, and wheat starch, or rapeseed oil, soybean oil, and corn.
- lipid sources selected from oil, pollack liver oil, and squid liver oil, and even when used in combination with the above-mentioned mixed feed, the effect of preventing or improving scutica infection of the feed additive of the present invention remains the same. do.
- the feed composition of the present invention may additionally contain binders, emulsifiers, preservatives, etc., and may additionally contain amino acids, vitamins, enzymes, probiotics, flavoring agents, nitrogen compounds, silicate agents, buffers, colorants, extractants, oligosaccharides, etc. It may be included, but is not limited to this.
- a method for preventing or treating scutica infection in flounder comprising administering to a fish farm a composition containing micronized liquid sulfur as an active ingredient.
- the composition of the present invention can be administered to the fish farm at 100 to 1,000 ppm, preferably at 150 to 500 ppm.
- the bathing (administration) time of the medicinal bath for flatfish in the fish farm can be 1 to 4 hours, and the number of bathing times can be set to 1 time/day.
- the interval between drug baths may be 2 to 3 days.
- the dosage of the composition of the present invention in a fish farm may vary depending on the weight of the treated fish, health status, administration time, and severity of disease.
- a method of preventing or treating scutica infection in flatfish comprising feeding the feed composition of the present invention to flatfish.
- the feeding amount of the feed composition can be the same as that fed during normal flounder breeding.
- composition of the present invention has an excellent effect in controlling Scootica disease in flounder, and can be safely and effectively used to prevent or treat Scotica infection in flounder, instead of the highly harmful formalin previously used to control Scootica disease in fish. there is.
- Figure 1 is a photograph before and after adding a surfactant to a mixture of sulfur powder and water.
- Figure 2 is a photograph showing an example of the manufacturing process of micronized sulfur powder.
- Figure 3 is an electron microscope photograph of a sulfur powder dispersion before wet mill grinding, after 2 hours of grinding, and after 4 hours of grinding.
- Figure 4 is a graph showing the change in sulfur particle size distribution as wet mill grinding time increases.
- Figure 5 is a graph showing the maximum particle size and average particle size of sulfur according to wet mill grinding time.
- Figure 6 is a photograph showing the exposure safety analysis test process of micronized liquid sulfur according to the present invention on flounder seeds.
- Figure 7 is a photograph showing the treatment of a composition (micronized liquid sulfur) according to the present invention by administering it to a tank of flounder infected with Scootica.
- Figure 8 is a photograph showing flounder seeds infected with Scootica worms.
- Figure 9 is a photograph showing flounder seeds from which Scootica worms were rescued after treatment with the composition of the present invention (micronized liquid sulfur).
- the maximum particle size of the sulfur particles in the dispersion before grinding with a wet mill, after grinding for 2 hours, and after grinding for 4 hours was reduced to 127 ⁇ m, 98 ⁇ m, and 76 ⁇ m, respectively.
- liquid sulfur was prepared in Comparative Example 1 by mixing 7.999 kg of water and 0.001 kg of surfactant in 2 kg of sulfur powder (maximum particle size 127 ⁇ m) before wet mill grinding and dispersing it by stirring.
- micronized liquid sulfur was administered to the aquarium under the following conditions and then exposed to flounder seeds for 48 hours. Survival and growth after two weeks of exposure were analyzed:
- Control (0ppm), 10mg/L (10ppm), 20mg/L (20ppm), 40mg/L (40ppm), 80mg/L (80ppm), 160mg/L (160ppm) treatment group
- the micronized liquid sulfur according to the present invention is safe for flatfish, and when the above results regarding flatfish seeds are converted to adult flatfish, it can be seen that the micronized liquid sulfur according to the present invention is safe even at 1000 ppm. there is.
- Test Example 2 Flatfish Scootica infection treatment effect analysis test
- Administer sample (Example 1 or Comparative Example 1) at a concentration of 150 ppm to water in the breeding tank.
- Example 1 the flounder seeds bathed with micronized liquid sulfur in Example 1 according to the present invention were all alive after four baths, and no scutica worms were observed in the wound area and body surface (FIG. 9).
- composition containing micronized liquid sulfur as an active ingredient according to the present invention is excellent in the extermination effect of scutica worms in flounder.
- Test Example 3 Flatfish seed growth test according to feed composition feeding
- a feed composition containing 1% by weight of micronized liquid sulfur of Example 1 prepared in Preparation Example 1 (treatment) and a feed composition not containing micronized liquid sulfur (control) were added to flounder seeds under the following conditions, respectively. After rearing while feeding, growth was analyzed by observing body length and weight, and the results are shown in Tables 1 and 2, respectively:
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Abstract
Provided in the present invention are a composition and a method for preventing or treating scuticociliatosis in Paralichthys olivaceus, the composition containing micronized liquid sulfur as an active ingredient. The composition of the present invention has the excellent effect of exterminating scuticociliates in Paralichthys olivaceus, and thus can be used, instead of highly harmful formalin, which is conventionally used for exterminating scuticociliatosis in fish, for the safe and effective prevention or treatment of scuticociliatosis in Paralichthys olivaceus.
Description
본 발명은 광어의 스쿠티카충 감염 예방 또는 치료 조성물 및 방법에 관한 것이다. 보다 상세하게는 미세화된 액상유황을 유효성분으로 포함하는 광어의 스쿠티카충 감염 예방 또는 치료용 조성물, 또는 예방 또는 개선용 사료 조성물, 상기 조성물을 광어에 직접 또는 양식장에 투여하는 단계를 포함하는 광어의 스쿠티카충 감염 예방 또는 치료 방법에 관한 것이다.The present invention relates to a composition and method for preventing or treating scutica infection in flounder. More specifically, a composition for preventing or treating scutica infection in flatfish containing micronized liquid sulfur as an active ingredient, or a feed composition for preventing or improving flatfish, comprising the step of administering the composition directly to flatfish or to a farm. It relates to a method of preventing or treating scutica infection.
어류양식어업은 수산자원의 고갈에 대항하는 혁신적인 대안으로 1990년대 중반부터 정부에서 적극 장려하고 있다. 그러나, 현재 우리나라 양식의 폐사율은 연간 20%를 상회하며, 그 주요원인으로 수온 상승으로 인한 난치성 질병 발생 및 혼합 감염 (바이러스, 세균, 기생충), 약제 치료효과의 저감이 지목되고 있다.Fish farming has been actively promoted by the government since the mid-1990s as an innovative alternative to combating the depletion of fisheries resources. However, the current mortality rate of aquaculture in Korea exceeds 20% per year, and the main causes are the occurrence of intractable diseases due to increased water temperature, mixed infections (viruses, bacteria, parasites), and reduced drug treatment effectiveness.
특히 양식어류에 스쿠티카충이 감염될 경우에 피해가 상당하여 대량 폐사의 원인이 되어 양식 산업에 있어서 큰 문제가 된다. 기생충성 감염 질환의 하나인 스쿠티카증은 육상의 약식수조나 해상가두리에서 양식되고 있는 해수 어류, 특히 광어 등에서 주로 발병하는 질병으로서 원생동물의 섬모충의 일종인 스쿠티코실리아티다목 (Scuticociliatids)에 속하는 기생충인 스쿠티카충이 광어의 피하조직에 침입하여 궤양을 일으킴으로서 발생한다. 이 충에 어체가 감염되면 종묘에서 지속적으로 증식하여 폐사율이 높아지며, 이 경우는 밀식을 피하고 유기물질이 수조 밑바닥에 남지 않도록 제거해 주어야 하며, 종래 사용되어왔던 치료방법으로는 스쿠티카충이 체표나 아가미 및 지느러미와 같은 외부에 기생했을 때 100~150ppm의 포르말린으로 1시간 정도 약욕하는 방법을 사용하여 왔다(한국특허등록 10-1719669호).In particular, when farmed fish are infected with Scotica worms, the damage is significant and causes mass mortality, which is a major problem in the aquaculture industry. Scuticosis, a parasitic infectious disease, is a disease that mainly occurs in marine fish, especially flounder, cultivated in aquatic tanks on land or in offshore cages. It belongs to the order Scuticociliatids, a type of protozoan ciliary worm. It occurs when the parasite Scootica invades the subcutaneous tissue of flatfish and causes ulcers. If the fish body is infected with this worm, it continues to proliferate in the seedlings, increasing the mortality rate. In this case, overcrowding should be avoided and organic matter should be removed so that it does not remain at the bottom of the tank. Conventionally used treatment methods include scutica worms being infected on the body surface, gills, and When parasites appear on external surfaces such as fins, a method of bathing them in 100 to 150 ppm formalin for about an hour has been used (Korean Patent Registration No. 10-1719669).
포르말린은 대표적 발암물질인 포름알데히드를 물에 녹여 35~38%의 농도로 만든 것이다. 이는 소독제, 방부제로 널리 쓰이며 국내에서는 수질환경보전법(제29조), 유해화학물질관리법(제15조), 폐기물관리법(제25조) 등에 따라 독극물로 분류돼 엄격한 관리를 요구받고 있고, 포름알데히드는 대기환경보전법(제2조)에 따라 유해물질로 분류된다.Formalin is made by dissolving formaldehyde, a representative carcinogen, in water to a concentration of 35-38%. It is widely used as a disinfectant and preservative, and in Korea, it is classified as a poison and requires strict management under the Water Quality Conservation Act (Article 29), the Toxic Chemicals Control Act (Article 15), and the Waste Management Act (Article 25). Formaldehyde is classified as a hazardous substance according to the Clean Air Conservation Act (Article 2).
유황은 산업적으로는 황산 제조, 니켈 제련, 고무의 가황, 화약, 성냥 및 의약품의 원료 등 다양한 용도로 활용되고 있다. 유황은 녹는점이 약 118℃로 석유제품의 유황분을 제거하는 탈황공정으로부터 추출되는 H2S 성분이 유황 회수시설을 거쳐 용융된 유황으로 생산된다. 용융된 유황은 보온시설이 잘 갖추어진 탱크에 132℃ 이상으로 유지한 상태로 수송되거나, 냉각 및 분쇄하여 유황분말로 제조되어 유통된다. 이때 제조된 유황분말은 통상적으로 최대입자크기가 127 ㎛ 이상으로 크다. Sulfur is used industrially for a variety of purposes, including manufacturing sulfuric acid, smelting nickel, vulcanizing rubber, and as a raw material for gunpowder, matches, and medicine. Sulfur has a melting point of about 118°C, and the H 2 S component extracted from the desulfurization process that removes sulfur content from petroleum products passes through a sulfur recovery facility and is produced as molten sulfur. Molten sulfur is transported in a state maintained above 132℃ in well-equipped tanks, or cooled and pulverized to produce sulfur powder and distributed. The sulfur powder produced at this time typically has a maximum particle size of 127 ㎛ or more.
따라서 스쿠티카충의 치료약으로서 포르말린의 사용은 물론 국내의 양식장에서도 더 이상 사용이 어렵게 되었으며, 이를 대체할 약제 조성물 등의 개발이 필요하나, 아직 특별한 대체조성물이 개발되지 않은 상태이며, 포르말린을 대체할 천연물 유래 스쿠티카충 구제제의 개발이 시급한 실정이다.Therefore, it has become difficult not only to use formalin as a treatment for scutica worms, but also to use it in domestic fish farms. It is necessary to develop a pharmaceutical composition to replace it, but a special replacement composition has not yet been developed, and natural products to replace formalin are needed. The development of an exterminator for Scootica worms is urgently needed.
본 발명의 목적은 미세화된 액상유황을 유효성분으로 포함하는 광어의 스쿠티카충 감염 예방 또는 치료용 조성물을 제공하는 것이다.The purpose of the present invention is to provide a composition for preventing or treating scutica infection in flatfish containing micronized liquid sulfur as an active ingredient.
본 발명의 또 다른 목적은 미세화된 액상유황을 사료첨가제로 포함하는 광어의 스쿠티카충 감염 예방 또는 개선용 사료 조성물을 제공하는 것이다.Another object of the present invention is to provide a feed composition for preventing or improving scutica infection in flatfish containing micronized liquid sulfur as a feed additive.
본 발명의 또 다른 하나의 목적은 미세화된 액상유황을 유효성분으로 포함하는 조성물을 양식장에 투여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법을 제공하는 것이다.Another object of the present invention is to provide a method for preventing or treating scutica infection in flounder, comprising administering to a fish farm a composition containing micronized liquid sulfur as an active ingredient.
본 발명의 또 다른 목적은 상기 사료 조성물을 광어에 급여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법을 제공하는 것이다.Another object of the present invention is to provide a method for preventing or treating scutica infection in flatfish, comprising feeding the feed composition to flatfish.
상기 과제를 해결하기 위한 하나의 양태로서, 본 발명은 미세화된 액상유황을 유효성분으로 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료용 조성물을 제공한다.As an aspect for solving the above problem, the present invention provides a composition for preventing or treating scutica infection in flounder, comprising micronized liquid sulfur as an active ingredient.
본 발명에서 미세화된 액상유황은 입자크기가 미세화된 유황을 의미하는 것으로, 유황의 최대입자크기가 10~60 ㎛, 바람직하게는 10~20 ㎛이며, 유황의 평균입자크가가 10 ㎛ 이하, 바람직하게는 5 ㎛ 이하이다. In the present invention, micronized liquid sulfur refers to sulfur whose particle size is micronized. The maximum particle size of sulfur is 10 to 60 ㎛, preferably 10 to 20 ㎛, and the average particle size of sulfur is 10 ㎛ or less. Preferably it is 5 μm or less.
본 발명에서 미세화된 액상유황은 유황분말 5~50 중량%, 계면활성제 0.01~0.05 중량% 및 나머지 물을 혼합하여 습식밀(wet mill)로 5시간 이상, 바람직하게는 22~100시간 분쇄하여 제조될 수 있다. In the present invention, the micronized liquid sulfur is prepared by mixing 5 to 50% by weight of sulfur powder, 0.01 to 0.05% by weight of surfactant, and the remaining water and grinding in a wet mill for more than 5 hours, preferably for 22 to 100 hours. It can be.
본 발명에서 계면활성제는 유황분말을 물에 고르게 분산되게 하는 역할을 하며, 식품, 화장품 또는 동물용 의약품에 사용되는 계면활성제가 사용될 수 있다. 구체적으로 계면활성제로는 알킬디메틸아민옥사이드, 폴리소르베이트계 (polysorbate 60, polysorbate 80), 소르비탄계(Sorbitan Laurate), POE(폴리옥시에틸렌)계, PEG(폴리에틸렌글리콜)계(PEG 80, PEG 100), AOS (Alpha Olefin Sulphonate), AE (Polyoxyethylene alkyl ether), SLS (Sodium lauryl sulfate), SLES (Sodium laureth sulfate), 트리스티릴페닐에테르인산, 폴리옥시에틸렌-폴리옥시프로필렌 코폴리머 레시틴(Lecithin), 코코베타인(COCO-BETAIN), 솔빌라이저(Solubilizer), 애플워시(Apple Wash), 이멀시파잉왁스 (Emulsifying Wax), 올리브 왁스(Olive Emulsifying Wax), 올리브 리퀴드(Olive oil peg-7 ESTERS), 바바수베타인(Babassuamidopropyl Betain), 코나코파(Decyl Glucoside), 트리아세틴(triacetin), 스테아릴젖산 칼슘 (Calcium stearoyl lactate), 다이소듐코코암포디아세테이트(Disodium Cocoamphodiacetate) 및 이의 혼합물로부터 선택될 수 있다.In the present invention, the surfactant serves to evenly disperse the sulfur powder in water, and surfactants used in food, cosmetics, or veterinary medicine can be used. Specifically, surfactants include alkyldimethylamine oxide, polysorbate (polysorbate 60, polysorbate 80), sorbitan (POE), POE (polyoxyethylene), and PEG (polyethylene glycol) (PEG 80, PEG). 100), AOS (Alpha Olefin Sulphonate), AE (Polyoxyethylene alkyl ether), SLS (Sodium lauryl sulfate), SLES (Sodium laureth sulfate), tristyrylphenyl ether phosphate, polyoxyethylene-polyoxypropylene copolymer lecithin (Lecithin) ), COCO-BETAIN, Solubilizer, Apple Wash, Emulsifying Wax, Olive Emulsifying Wax, Olive Oil peg-7 ESTERS ), Babassuamidopropyl Betain, Conacopa (Decyl Glucoside), triacetin, Calcium stearoyl lactate, Disodium Cocoamphodiacetate and mixtures thereof. You can.
본 발명의 일 실시예에서는 계면활성제로 트리스티릴페닐에테르인산( Tristyrylphenyl etherphosphate)을 사용하였다 (제조예 1). In one example of the present invention, tristyrylphenyl etherphosphate was used as a surfactant (Preparation Example 1).
본 발명에서 상기와 같이 미세화된 액상유황은 광어의 스쿠티카충의 치료 효과가 있음이 확인되었다 (시험예 2). In the present invention, it was confirmed that the micronized liquid sulfur as described above is effective in treating scutica worms in flounder (Test Example 2).
본 발명에서 스쿠티카충은 양식어에 기생하는 섬모충으로 양식중인 광어 치어에 기생하여 큰 피해를 준다. 스쿠티카충은 광어 스쿠티카증을 유발하는데, 스쿠티카증은 30~40㎛의 스쿠티카 섬모충에 의해 발생되며 육상의 양식수조나 해상가두리에서 양식되고 있는 해수 어류, 특히 광어의 인공종묘 및 양성어에 감염되며 감염 부위도 체표와 지느러미 그리고 간장, 혈관 및 뇌조직까지의 심부 감염을 유발하고, 대부분 소성결합조직의 파괴를 통하여 광어를 대량 폐사시킨다. 이 스쿠티카 섬모충은 다른 어류의 기생충과는 달리 혈관을 통하여 뇌조직까지 침입하여 기생하기 때문에 감염시 치료가 어려운 질병이다.In the present invention, Scootica is a ciliate parasite that parasitizes farmed fish and causes great damage to flounder fry being farmed. Scootica causes flounder scotiosis, which is caused by 30-40㎛ Scootica ciliates and is used in marine fish cultured in aquaculture tanks on land or in offshore cages, especially artificial seedlings and breeding fish of flatfish. It causes deep infection in the body surface, fins, liver, blood vessels, and brain tissue, and causes mass death of flounder mostly through destruction of plastic connective tissue. Unlike other fish parasites, this scutica ciliate is a disease that is difficult to treat when infected because it invades brain tissue through blood vessels and becomes parasitic.
본 발명에 따른 조성물은 광어의 스쿠티카충 감염 예방 및 치료 용도에 적합하다. The composition according to the present invention is suitable for preventing and treating scutica infection in flounder.
본 발명에서 예방은 상기 조성물에 의해 광어의 스쿠티카충 감염을 억제하거나 발병을 지연시키는 모든 행위를 의미하며, 치료는 상기 조성물에 의해 광어의 스쿠티카충 감염에 의한 증세가 호전되거나 이롭게 변경되는 모든 행위를 의미한다.In the present invention, prevention refers to any action that suppresses or delays the onset of scutica infection in flatfish by the composition, and treatment refers to any action that improves or beneficially changes the symptoms caused by scutica infection in flatfish by the composition. means action.
본 발명에서 조성물은 각각 통상의 방법에 따라 다양한 형태로 제형화하여 사용될 수 있으며, 어류에 사용되므로 약욕제의 제형으로 제공되는 것이 가장 바람직할 수 있다.In the present invention, the composition can be formulated and used in various forms according to conventional methods, and since it is used on fish, it may be most preferable to provide it in the form of a medicated bath.
또한, 각각의 제형에 따라 수의학적으로 허용가능한 담체, 예컨대 완충제, 보존제, 무통화제, 가용화제, 등장제, 안정화제, 기제, 부형제, 윤활제 등 당업계에 공지된 담체를 추가로 포함하여 제조할 수 있다. In addition, each formulation may be prepared by additionally including veterinary acceptable carriers such as buffers, preservatives, analgesics, solubilizers, isotonic agents, stabilizers, bases, excipients, lubricants, etc. known in the art. You can.
본 발명에 따른 조성물은 수의학적으로 유효한 양으로 투여한다. 수의학적으로 유효한 양은 수의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분하며 부작용을 일으키지 않을 정도의 양을 의미하며, 유효용량 수준은 어류의 건강상태, 중증도, 약물의 활성, 약물에 대한 민감도, 투여 방법, 투여 시간, 투여 경로 및 배출 비율, 치료기간, 배합 또는 동시 사용되는 약물을 포함한 요소 및 기타 수의학 분야에 잘 알려진 요소에 따라 결정될 수 있다. The composition according to the present invention is administered in a veterinary effective amount. A veterinary effective amount refers to an amount that is sufficient to treat a disease with a reasonable benefit/risk ratio applicable to veterinary treatment and does not cause side effects. The effective dose level refers to the health status of the fish, severity, activity of the drug, It can be determined based on factors including sensitivity to the drug, method of administration, time of administration, route of administration and excretion rate, duration of treatment, drugs combined or used simultaneously, and other factors well known in the veterinary field.
본 발명에서 조성물이 약욕제의 제형으로 제공될 경우, 100 ~ 1,000 ppm 으로, 바람직하게는 150 ~ 500 ppm으로 양식장(수조)에 투여할 수 있다. 광어에 대한 약욕제의 약욕 시간은 1 ~ 4 시간, 약욕 횟수는 1회/일로 할 수 있다. 약욕 간격은 2~3일일 수 있다. In the present invention, when the composition is provided in the form of a medicated bath, it can be administered to the fish farm (water tank) at 100 to 1,000 ppm, preferably at 150 to 500 ppm. The bathing time of the medicinal bath for flatfish can be 1 to 4 hours, and the number of bathing times can be 1 time/day. The interval between drug baths may be 2 to 3 days.
본 발명의 또 다른 목적에 따라서, 미세화된 액상유황을 사료첨가제로 포함하는 광어의 스쿠티카충 감염 예방 또는 개선용 사료 조성물을 제공한다. According to another object of the present invention, a feed composition for preventing or improving scutica infection in flatfish containing micronized liquid sulfur as a feed additive is provided.
본 발명의 사료 조성물에서 미세화된 액상유황은 0.01 내지 3 중량%, 바람직하게는 0.01 내지 1 중량%으로 포함될 수 있다. 미세화된 액상유황이 상기 범위 미만일 때는 스쿠티카충에 대한 살충 효능이 미약하고, 상기 범위를 초과하는 경우에는 물질 자체의 독성이 문제될 수 있다. In the feed composition of the present invention, micronized liquid sulfur may be included in an amount of 0.01 to 3% by weight, preferably 0.01 to 1% by weight. When the micronized liquid sulfur is less than the above range, the insecticidal efficacy against scutica insects is weak, and when it exceeds the above range, the toxicity of the substance itself may be a problem.
사료 첨가제는 영양적 또는 특정 목적을 위하여 사료에 미량으로 첨가되는 물질을 총칭하는 것으로, 본 발명에서는 어류의 스쿠티카충 감염 예방 또는 개선을 목적으로 첨가되는 물질을 의미한다. 본 발명의 미세화된 액상유황은 사료 첨가제로서 사료 조성물에 포함될 수 있다. Feed additives are a general term for substances added in small amounts to feed for nutritional or specific purposes. In the present invention, they refer to substances added for the purpose of preventing or improving scutica infection in fish. The micronized liquid sulfur of the present invention can be included in the feed composition as a feed additive.
사료는 어류에 공급되는 모든 식품을 총칭한다. 사료 조성물에 통상적으로 사용되는 배합사료로는 어분, 카제인, 육골분, 새우분말 중에서 선택되는 어느 하나 이상의 단백질원 또는 고구마 전분, 감자전분, 밀 전분 중에서 선택되는 어느 하나 이상의 탄수화물원 또는 채종유, 대두유, 옥수수유, 명태간유, 오징어간유 중에서 선택되는 어느 하나 이상의 지질원 등이 있으나 이에 한정되는 것은 아니며, 상기 배합사료와 배합하여 사용하더라도 본 발명의 사료 첨가제의 스쿠티카충 감염 예방 또는 개선 효과는 동일하게 발생된다. Feed refers to all foods supplied to fish. Formulated feeds commonly used in feed compositions include one or more protein sources selected from fish meal, casein, meat and bone meal, and shrimp powder, or one or more carbohydrate sources selected from sweet potato starch, potato starch, and wheat starch, or rapeseed oil, soybean oil, and corn. There is, but is not limited to, one or more lipid sources selected from oil, pollack liver oil, and squid liver oil, and even when used in combination with the above-mentioned mixed feed, the effect of preventing or improving scutica infection of the feed additive of the present invention remains the same. do.
본 발명의 사료 조성물에는 결착제, 유화제, 보존제 등을 추가로 포함될 수 있고, 아미노산제, 비타민제, 효소제, 생균제, 향미제, 질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등을 추가로 포함될 수 있으나, 이에 한정된 것은 아니다.The feed composition of the present invention may additionally contain binders, emulsifiers, preservatives, etc., and may additionally contain amino acids, vitamins, enzymes, probiotics, flavoring agents, nitrogen compounds, silicate agents, buffers, colorants, extractants, oligosaccharides, etc. It may be included, but is not limited to this.
본 발명의 또 다른 목적에 따라서, 미세화된 액상유황을 유효성분으로 포함하는 조성물을 양식장에 투여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법을 제공한다.According to another object of the present invention, there is provided a method for preventing or treating scutica infection in flounder, comprising administering to a fish farm a composition containing micronized liquid sulfur as an active ingredient.
상기 방법에서 본 발명의 조성물은 100 ~ 1,000 ppm 으로, 바람직하게는 150 ~ 500 ppm으로 양식장에 투여할 수 있다. 상기 방법에서 양식장에서의 광어의 약욕제의 약욕(투여) 시간은 1 ~ 4 시간, 약욕 횟수는 1회/일로 할 수 있다. 약욕 간격은 2~3일일 수 있다. 본 발명의 조성물의 양식장에 투여량은 피처리 어류의 중량, 건강상태, 투여시간, 질환의 중증도에 따라 그 범위를 달리할 수 있다. In the above method, the composition of the present invention can be administered to the fish farm at 100 to 1,000 ppm, preferably at 150 to 500 ppm. In the above method, the bathing (administration) time of the medicinal bath for flatfish in the fish farm can be 1 to 4 hours, and the number of bathing times can be set to 1 time/day. The interval between drug baths may be 2 to 3 days. The dosage of the composition of the present invention in a fish farm may vary depending on the weight of the treated fish, health status, administration time, and severity of disease.
본 발명의 또 다른 목적에 따라서, 본 발명의 사료 조성물을 광어에 급여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법을 제공한다. According to another object of the present invention, there is provided a method of preventing or treating scutica infection in flatfish, comprising feeding the feed composition of the present invention to flatfish.
사료 조성물의 급여량은 통상의 광어 사육시 급여하는 양으로 수행할 수 있다. The feeding amount of the feed composition can be the same as that fed during normal flounder breeding.
본 발명의 조성물은 광어의 스쿠티카충 구제 효과가 우수하여, 종래 어류의 스쿠티카병 구제를 위하여 사용되었던 유해성이 높은 포르말린을 대신하여, 안전하고 효과적으로 광어의 스쿠티카충 감염 예방 또는 치료에 사용될 수 있다.The composition of the present invention has an excellent effect in controlling Scootica disease in flounder, and can be safely and effectively used to prevent or treat Scotica infection in flounder, instead of the highly harmful formalin previously used to control Scootica disease in fish. there is.
도 1은 유황분말과 물의 혼합물에 계면활성제 첨가 전과 후의 사진이다.Figure 1 is a photograph before and after adding a surfactant to a mixture of sulfur powder and water.
도 2는 미세화된 유황분말의 제조 공정의 일례를 보여주는 사진이다. Figure 2 is a photograph showing an example of the manufacturing process of micronized sulfur powder.
도 3은 유황분말 분산액의 습식밀 분쇄 전, 2시간 분쇄 후, 4시간 분쇄 후의 전자현미경 사진이다. Figure 3 is an electron microscope photograph of a sulfur powder dispersion before wet mill grinding, after 2 hours of grinding, and after 4 hours of grinding.
도 4는 습식밀 분쇄 시간이 증가함에 따른 유황 입자크기의 분포 변화를 보여주는 그래프이다. Figure 4 is a graph showing the change in sulfur particle size distribution as wet mill grinding time increases.
도 5는 습식밀 분쇄 시간에 따른 유황의 최대입자크기와 평균입자크기를 보여주는 그래프이다. Figure 5 is a graph showing the maximum particle size and average particle size of sulfur according to wet mill grinding time.
도 6은 광어종자에 대한 본 발명에 따른 미세화된 액상유황의 노출 안전성 분석 시험 과정을 보여주는 사진이다. Figure 6 is a photograph showing the exposure safety analysis test process of micronized liquid sulfur according to the present invention on flounder seeds.
도 7은 본 발명에 따른 조성물(미세화된 액상유황)을 스쿠티카충에 감염된 광어의 수조에 투여하여 처리하는 모습을 보여주는 사진이다. Figure 7 is a photograph showing the treatment of a composition (micronized liquid sulfur) according to the present invention by administering it to a tank of flounder infected with Scootica.
도 8은 스쿠티카충에 감염된 광어 종자를 보여주는 사진이다.Figure 8 is a photograph showing flounder seeds infected with Scootica worms.
도 9는 본 발명의 조성물(미세화된 액상유황) 처리 후 스쿠티카충이 구제된 광어 종자를 보여주는 사진이다.Figure 9 is a photograph showing flounder seeds from which Scootica worms were rescued after treatment with the composition of the present invention (micronized liquid sulfur).
이하, 본 발명의 이해를 돕기 위하여 구체적인 실시예를 통하여 본 발명의 구성 및 효과를 보다 상세히 설명하기로 한다. 그러나 하기 실시예 및 실험예는 본 발명을 보다 명확하게 이해시키기 위하여 예시한 것일 뿐이며, 본 발명의 권리범위가 하기 실시예에 의해 한정되는 것은 아니다. Hereinafter, in order to facilitate understanding of the present invention, the configuration and effects of the present invention will be described in more detail through specific examples. However, the following examples and experimental examples are merely illustrative for a clearer understanding of the present invention, and the scope of the present invention is not limited by the following examples.
제조예 1: 미세화 액상유황의 제조 및 최적 조건 도출Preparation Example 1: Preparation of micronized liquid sulfur and derivation of optimal conditions
유황분말 ((주)에이치설퍼, 최대입자크기 127 ㎛) 2 kg에 물 7.999 kg 과 계면활성제 0.001 kg (Tristyrylphenyl etherphosphate, ㈜이룸켐엔택. ER-SC480)을 혼합하고 교반하여 분산시켰다 (도 1). 2 kg of sulfur powder (H Sulfur Co., Ltd., maximum particle size 127 ㎛) was mixed with 7.999 kg of water and 0.001 kg of surfactant (Tristyrylphenyl etherphosphate, Erum Chem Entech, Inc. ER-SC480) and stirred to disperse (Figure 1 ).
분산액을 교반하면서 습식밀 (800 ~ 1,000 rpm)로 2시간을 1사이클로 하여 유황입자를 분쇄하였으며, 사이클을 반복하여 최종 98시간까지 유황입자를 분쇄하였으며 (도 2), 각 사이클마다 입도분석기로 입자크기 변화를 분석하였고 그 결과를 도 3 ~ 도 5에 나타냈다. While stirring the dispersion, the sulfur particles were pulverized using a wet mill (800 ~ 1,000 rpm) for one cycle of 2 hours. The cycle was repeated until the final 98 hours were pulverized (Figure 2), and the particles were analyzed with a particle size analyzer for each cycle. The size change was analyzed and the results are shown in Figures 3 to 5.
도 3에 도시된 바와 같이, 분산액의 유황 입자는 습식밀로 분쇄전, 2시간 분쇄 후, 4시간 분쇄 후 최대입자크기는 각각 127 ㎛, 98 ㎛, 76 ㎛로 작아졌다. As shown in Figure 3, the maximum particle size of the sulfur particles in the dispersion before grinding with a wet mill, after grinding for 2 hours, and after grinding for 4 hours was reduced to 127 ㎛, 98 ㎛, and 76 ㎛, respectively.
도 4에 도시된 바와 같이, 습식밀로 분쇄 시간이 늘어날수록 유황 입자크기분포는 작아졌으며, 분쇄시간이 98시간 초과해도 더 이상 유황 입자크기분포는 작아지지 않았다. 유황의 최대입자크기의 임계치는 10 ㎛ 이상인 것으로 확인되었다. As shown in Figure 4, as the grinding time with the wet mill increased, the sulfur particle size distribution became smaller, and even when the grinding time exceeded 98 hours, the sulfur particle size distribution no longer became smaller. The critical value of the maximum particle size of sulfur was confirmed to be 10 ㎛ or more.
도 5에 도시된 바와 같이, 습식밀 분쇄는 5시간 이상 수행시 유황 최대입자크기가 60 ㎛ 이하, 유황 평균입자크기가 10 ㎛ 이하가 되어서 본 발명에 따른 미세화된 액상유황이 제조될 수 있음이 확인되었다. 습식밀 분쇄를 22 ~ 100 시간 수행시 유황 최대입자크기가 20 ㎛ 이하, 유황 편균입자크기가 5 ㎛가 되어서 본 발명에 따른 미세화된 액상유황으로 가장 바람직함이 확인되었다. As shown in Figure 5, when wet mill grinding is performed for more than 5 hours, the maximum sulfur particle size becomes 60 ㎛ or less and the average sulfur particle size becomes 10 ㎛ or less, indicating that finely divided liquid sulfur according to the present invention can be produced. Confirmed. When wet mill grinding was performed for 22 to 100 hours, the maximum sulfur particle size was 20 ㎛ or less and the average sulfur particle size was 5 ㎛, confirming that the micronized liquid sulfur according to the present invention is most desirable.
상기와 같이 유황분말 2 kg에 물 7.999 kg 과 계면활성제 0.001 kg을 혼합하고 교반하여 분산시킨 분산액을 습식밀로 22시간 분쇄하여 제조한 미세화된 액상유황을 사용하여 하기 시험예에서 분석하였다 (실시예 1). As described above, 2 kg of sulfur powder was mixed with 7.999 kg of water and 0.001 kg of surfactant, stirred, and dispersed. Micronized liquid sulfur prepared by pulverizing the dispersion with a wet mill for 22 hours was analyzed in the following test example (Example 1 ).
비교를 위하여, 습식밀 분쇄전 유황분말 (최대입자크기 127 ㎛) 2 kg에 물 7.999 kg 과 계면활성제 0.001 kg을 혼합하고 교반하여 분산시킨 액상유황을 비교예 1로 준비하였다. For comparison, liquid sulfur was prepared in Comparative Example 1 by mixing 7.999 kg of water and 0.001 kg of surfactant in 2 kg of sulfur powder (maximum particle size 127 ㎛) before wet mill grinding and dispersing it by stirring.
시험예 1: 미세화된 액상유황의 안전성 분석 시험Test Example 1: Safety analysis test of micronized liquid sulfur
광어종자에 대한 상기 제조예 1에서 제조된 미세화된 액상유황 (실시예 1)의 안전성분석을 제주대학교 해양연구소에서 수행하였다 (도 6). Safety analysis of micronized liquid sulfur (Example 1) prepared in Preparation Example 1 for flatfish seeds was performed at the Jeju National University Marine Research Institute (FIG. 6).
구체적으로는 하기와 같은 조건으로 미세화된 액상유황을 수조에 투여한 후 광어종자에 48시간 노출시킨 후 생존 여부와, 노출 2주 후의 성장 정도를 분석하였다: Specifically, micronized liquid sulfur was administered to the aquarium under the following conditions and then exposed to flounder seeds for 48 hours. Survival and growth after two weeks of exposure were analyzed:
- 실험어 : 광어 종자 (체장 17.3±0.9 cm, 체중 56.5±12.5g)- Experimental fish: flatfish seed (length 17.3±0.9 cm, weight 56.5±12.5g)
- 사육방법 : 80L 사육 수조에 5마리씩 침지- Breeding method: 5 fish each in an 80L breeding tank.
- 처리(투여) 농도 : 대조구(0ppm), 10mg/L(10ppm), 20mg/L(20ppm), 40mg/L(40ppm), 80mg/L(80ppm), 160mg/L(160ppm) 처리구 - Treatment (administration) concentration: Control (0ppm), 10mg/L (10ppm), 20mg/L (20ppm), 40mg/L (40ppm), 80mg/L (80ppm), 160mg/L (160ppm) treatment group
- 처리(노출) 기간 : 48시간- Processing (exposure) period: 48 hours
48시간 노출 시험 후 대조구와 처리구 모두 광어종자가 100% 생존하여 생존율에 차이가 없었고, 노출 2주 후 대조구와 처리구 광어종자의 성장 차이도 확인되지 않았다.After the 48-hour exposure test, 100% of the flounder seeds survived in both the control and treatment groups, so there was no difference in survival rate, and after 2 weeks of exposure, there was no difference in growth between the flounder seeds in the control and treatment groups.
따라서 본 발명에 따른 미세화된 액상유황은 광어에 대해 안전성을 가짐을 확인할 수 있으며, 광어 종자에 관한 상기 결과를 다자란 광어에 대해 환산하면 본 발명에 따른 미세화된 액상유황은 1000ppm 에서도 안전함을 알 수 있다. Therefore, it can be confirmed that the micronized liquid sulfur according to the present invention is safe for flatfish, and when the above results regarding flatfish seeds are converted to adult flatfish, it can be seen that the micronized liquid sulfur according to the present invention is safe even at 1000 ppm. there is.
시험예 2: 광어 스쿠티카충 감염 치료효과 분석 시험Test Example 2: Flatfish Scootica infection treatment effect analysis test
하기와 같은 조건으로 스쿠티카충에 감염된 광어 종자에 제조예 1에서 제조된 액상유황인 실시예 1 및 비교예 1을 각각 처리(약욕)시켜 스쿠티카충 감염에 대한 치료효과를 분석하였다: The treatment effect on Scootica infection was analyzed by treating (bathing) flounder seeds infected with Scootica worms with Example 1 and Comparative Example 1, the liquid sulfur prepared in Preparation Example 1, respectively, under the following conditions:
- 시험어 : 스쿠티카충에 감염된 광어 종자 (체장 19.3±0.8 cm, 체중 74.3±7.9g)- Test fish: Flatfish seed infected with Scootica worm (length 19.3±0.8 cm, weight 74.3±7.9g)
- 사육방법 : 2톤 사육수조 (Φ3.0x0.3m)에 30마리씩- Breeding method: 30 animals each in a 2-ton breeding tank (Φ3.0x0.3m)
- 투여농도 : 사육수조 물에 시료 (실시예 1 또는 비교예 1) 150ppm 농도로 투여- Administration concentration: Administer sample (Example 1 or Comparative Example 1) at a concentration of 150 ppm to water in the breeding tank.
- 처리(약욕) 횟수 : 2시간/일, 2일 간격으로 4회 - Number of treatments (medicinal bathing): 2 hours/day, 4 times at 2-day intervals
비교예 1의 액상유황으로 약욕시킨 광어 종자는 4회 약욕이 완료되기 전에 대부분 폐사되었고, 살아남은 광어 종자도 스쿠티카충의 감염이 거의 그대로 남아 있었다 (도 8).Most of the flounder seeds bathed with liquid sulfur in Comparative Example 1 died before the four baths were completed, and the surviving flounder seeds also remained largely infected with scutica worms (FIG. 8).
이에 비하여, 본 발명에 따른 실시예 1의 미세화된 액상유황으로 약욕시킨 광어 종자는 4회 약욕 완료 후까지 모두 살아있었으며, 상처 부위와 체표면에서는 스쿠티카충이 관찰되지 않았다(도 9). In contrast, the flounder seeds bathed with micronized liquid sulfur in Example 1 according to the present invention were all alive after four baths, and no scutica worms were observed in the wound area and body surface (FIG. 9).
따라서 본 발명에 따른 미세화된 액상유황을 유효성분으로 포함하는 조성물은 광어에서 스쿠티카충의 구제 효과가 우수함을 확인할 수 있다. Therefore, it can be confirmed that the composition containing micronized liquid sulfur as an active ingredient according to the present invention is excellent in the extermination effect of scutica worms in flounder.
시험예 3: 사료 조성물 급여에 따른 광어종자 성장 시험Test Example 3: Flatfish seed growth test according to feed composition feeding
하기와 같은 조건으로 광어 종자에 제조예 1에서 제조된 실시예 1의 미세화된 액상유황을 1중량% 포함하는 사료 조성물 (처리구)과, 미세화된 액상유황을 포함하지 않는 사료 조성물 (대조구)을 각각 급여하면서 사육한 후 체장과 체중 관찰을 통하여 성장을 분석하였고, 그 결과를 각각 표 1과 표 2에 나타냈다: A feed composition containing 1% by weight of micronized liquid sulfur of Example 1 prepared in Preparation Example 1 (treatment) and a feed composition not containing micronized liquid sulfur (control) were added to flounder seeds under the following conditions, respectively. After rearing while feeding, growth was analyzed by observing body length and weight, and the results are shown in Tables 1 and 2, respectively:
- 시험어 : 광어 종자 (체장 19.3±0.8 cm, 체중 74.3±7.9g)- Test fish: flatfish seed (length 19.3±0.8 cm, weight 74.3±7.9g)
- 사육방법 : 2톤 사육수조 (Φ3.0x0.3m)에 50마리씩 입식- Breeding method: Stocking 50 animals each in a 2-ton breeding tank (Φ3.0x0.3m)
- 사육일 : 14일- Raising date: 14 days
| 체장(cm)Length (cm) | 사육 전Before breeding | 2주 사육 후 After 2 weeks of rearing | 증감 increase |
| 대조구Control | 19.3±0.919.3±0.9 | 19.5±0.919.5±0.9 | 0.3 cm 증가 0.3 cm increase |
| 처리구treatment area | 19.2±0.819.2±0.8 | 19.8±1.019.8±1.0 | 0.6 cm 증가 0.6cm increase |
| 체중(g)Weight (g) | 사육 전Before breeding | 2주 사육 후 After 2 weeks of rearing | 증감 increase |
| 대조구Control | 74.7±9.774.7±9.7 | 84.0±9.684.0±9.6 | 9.3g 증가9.3g increase |
| 처리구treatment area | 73.9±6.173.9±6.1 | 84.5±9.184.5±9.1 | 10.6g 증가10.6g increase |
대조구와 처리구 간의 광어 종자의 성장을 비교해 보면 처리구가 체장과 체중이 더 증가하였으나, 유의적인 차이는 없었다. Comparing the growth of flounder seeds between the control and treatment groups, the treatment group increased length and body weight, but there was no significant difference.
Claims (13)
- 미세화된 액상유황을 유효성분으로 포함하는 광어의 스쿠티카충 감염 예방 또는 치료용 조성물.A composition for preventing or treating scutica infection in flounder, comprising micronized liquid sulfur as an active ingredient.
- 제 1항에 있어서, 미세화된 액상유황은 유황의 최대입자크기가 10~60 ㎛ 및 평균입자크기가 10 ㎛ 이하인 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물.The composition for preventing or treating scutica infection in flounder according to claim 1, wherein the micronized liquid sulfur has a maximum particle size of 10 to 60 ㎛ and an average particle size of 10 ㎛ or less.
- 제 2항에 있어서, 미세화된 액상유황은 유황의 최대입자크기가 10~20 ㎛ 및 평균입자크기가 5 ㎛ 이하인 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물.The composition of claim 2, wherein the micronized liquid sulfur has a maximum particle size of 10 to 20 ㎛ and an average particle size of 5 ㎛ or less.
- 제 1항에 있어서, 미세화된 액상유황은 유황분말 5~50 중량%, 계면활성제 0.01~0.05 중량% 및 나머지 물을 혼합하여 습식밀로 5~100시간 분쇄되어 제조된 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물. The method of claim 1, wherein the micronized liquid sulfur is prepared by mixing 5 to 50% by weight of sulfur powder, 0.01 to 0.05% by weight of surfactant and the remaining water and grinding it with a wet mill for 5 to 100 hours. Composition for prevention or treatment.
- 제 4항에 있어서, 미세화된 액상유황은 습식밀로 22~100시간 분쇄되어 제조된 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물. The composition for preventing or treating scutica infection in flounder according to claim 4, wherein the micronized liquid sulfur is prepared by grinding with wet wheat for 22 to 100 hours.
- 제 1항에 있어서, 상기 조성물은 약욕제 제형인 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물. The composition of claim 1, wherein the composition is a medicinal bath formulation for preventing or treating scutica infection in flounder.
- 제 6항에 있어서, 약욕제는 100 ~ 1,000 ppm 으로 양식장에 투여되고 약욕은 1회 1 ~ 4 시간 수행되는 것인 광어의 스쿠티카충 감염 예방 또는 치료용 조성물. The composition for preventing or treating scutica infection in flatfish according to claim 6, wherein the medicinal bath is administered to the fish farm at 100 to 1,000 ppm and the medicinal bath is performed once for 1 to 4 hours.
- 미세화된 액상유황을 사료첨가제로 포함하는 광어의 스쿠티카충 감염 예방 또는 개선용 사료 조성물로, A feed composition for preventing or improving scutica infection in flatfish containing micronized liquid sulfur as a feed additive,상기 미세화된 액상유황은 유황의 최대입자크기가 10~60 ㎛ 및 평균입자크기가 10 ㎛ 이하인 것인 사료 조성물. The micronized liquid sulfur is a feed composition in which the maximum particle size of sulfur is 10 to 60 ㎛ and the average particle size is 10 ㎛ or less.
- 제 8항에 있어서, 상기 사료 조성물에서 미세화된 액상유황은 0.01 내지 3 중량%로 포함되는 것인 광어의 스쿠티카충 감염 예방 또는 개선용 사료 조성물. The feed composition for preventing or improving scutica infection in flatfish according to claim 8, wherein the micronized liquid sulfur is contained in an amount of 0.01 to 3% by weight in the feed composition.
- 미세화된 액상유황을 유효성분으로 포함하는 조성물을 양식장에 투여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법으로, A method for preventing or treating scutica infection in flounder, comprising administering to a fish farm a composition containing micronized liquid sulfur as an active ingredient,상기 미세화된 액상유황은 유황의 최대입자크기가 10~60 ㎛ 및 평균입자크기가 10 ㎛ 이하인 것인 방법. The micronized liquid sulfur has a maximum particle size of 10 to 60 ㎛ and an average particle size of 10 ㎛ or less.
- 제 10항에 있어서, 상기 조성물은 100 ~ 1,000 ppm 으로 양식장에 투여되는 것인 광어의 스쿠티카충 감염 예방 또는 치료 방법. The method of claim 10, wherein the composition is administered to the fish farm at 100 to 1,000 ppm.
- 제 10에 있어서, 양식장에서의 광어의 약욕 시간은 1 ~ 4 시간/일, 약욕 간격은 2~3일인 것인, 광어의 스쿠티카충 감염 예방 또는 치료 방법. The method of claim 10, wherein the bathing time of flatfish in the fish farm is 1 to 4 hours/day, and the bathing interval is 2 to 3 days.
- 제 8항에 따른 사료 조성물을 광어에 급여하는 단계를 포함하는, 광어의 스쿠티카충 감염 예방 또는 치료 방법.A method of preventing or treating scutica infection in flatfish, comprising feeding the feed composition according to claim 8 to flatfish.
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| KR101850697B1 (en) * | 2017-08-29 | 2018-05-31 | 한국해양과학기술원 | Composition for preventing or treating scuticociliatosis in fishes, and natural feed having the same |
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| KR101850697B1 (en) * | 2017-08-29 | 2018-05-31 | 한국해양과학기술원 | Composition for preventing or treating scuticociliatosis in fishes, and natural feed having the same |
| KR101917564B1 (en) * | 2018-02-09 | 2018-11-09 | 유진형 | Method for exterminating scutica |
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| " Report, Maritime and Fisheries Research and Development Program.", 1 July 2017, NDSL, Korea, article THE HEAD OF THE RESEARCH - HEEJEONG LEE: "Grant no. 20150337- Development of Eco-friendly Treatment Agents that Are Effective against Aquatic Bacteria in Fish and Bacterium by Using decontaminated Natural Sulfur.", pages: 1 - 156, XP009554267 * |
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