WO2023274430A2

WO2023274430A2 - Method for prolonging storage period of egg masses of telenomus remus nixon

Info

Publication number: WO2023274430A2
Application number: PCT/CN2022/127370
Authority: WO
Inventors: 黄保宏; 王增霞; 蔡清华; 武德功; 杜军利
Original assignee: 安徽科技学院
Priority date: 2022-01-18
Filing date: 2022-10-25
Publication date: 2023-01-05
Also published as: ZA202300354B; CN114403129A; CN114403129B; WO2023274430A3

Abstract

Disclosed in the present invention is a method for prolonging the storage period of egg masses of Telenomus remus Nixon. The method involves materials and a reaction device. The material components are, in parts by weight: 5-10 parts of a thickening agent, 1-3 parts of an inorganic salt, 30-40 parts of sodium alginate, 20-30 parts of carrageenan, 5-10 parts of an inorganic antibacterial agent, 40-50 parts of egg white, 3-6 parts of quick lime, 2-4 parts of a preservative and 1-3 parts of cellulase. 30-40 parts of sodium alginate, 20-30 parts of carrageenan and 40-50 parts of egg white are contained inside the reaction device, and the reaction device is a main outer frame mounting structure of an apparatus. In the method for prolonging the storage period of egg masses of Telenomus remus Nixon, the raw materials are formed into a semi-solid material, and egg masses are placed inside same. The semi-solid material has a good cold absorption property, can cooperatively maintain an internal low-temperature state, and can prolong the incubation time of the egg masses. In addition, the raw materials contain nutrient substances, such that the success rate of eclosion of the egg masses is increased.

Description

A method for prolonging the storage period of the egg mass of Noctuid spp.

technical field

The invention relates to the technical field of insect breeding, in particular to a method for prolonging the storage period of egg masses of the noctuid moth.

Background technique

The wasp is an important parasitic natural enemy in the egg stage of a variety of Lepidoptera Noctuidae insects. Because the wasp has high parasitic performance and is easy to reproduce in large numbers, it makes It has become one of the main biological factors in the control of Lepidoptera Noctuidae pests. As early as the 1980s, Venezuela had multiplied Noctuid moth and used it to prevent and control Spodoptera frugiperda. Among them, in areas with high humidity, in corn fields The highest parasitism rate of eggs can reach 90%. In Mexico, in the corn-soybean, corn-pumpkin, corn-soybean-pumpkin and single corn planting areas, through the quantitative release of Noctuid spp. The parasitism rate of moth eggs shows that it is found that the parasitism rate and the released amount conform to the Poisson regression relationship, which has a significant biological control effect. In Latin America and Central America, such as Honduras, Dominica, Peru, Bolivia and Cuba, noctuid Black egg bee is widely used in the control of fall armyworm. In Venezuela and Colombia, the black egg bee is used as an important biological control product to control fall armyworm. Fall armyworm invades Africa Later, through egg molecular identification and population survey in South Africa, East Africa and West Africa, it was shown that the noctuid moth wasp is an important egg parasitoid of Spodoptera frugiperda in Africa, and it can be used as an important biological control parasitoid for the control of Spodoptera frugiperda.

However, the successful application of parasitoids in biological control is mainly achieved through field release. However, field release requires a large number of parasitoids as the basis, and a large number of parasitoids are required to be released at the same time, and the process of multi-batch breeding and eclosion takes a long time. , the preparation time for the future is relatively short, the flexibility is low, and it is difficult to regulate the development process of the egg mass of the noctuid moth, therefore, a method for prolonging the storage period of the egg mass of the noctuid moth is needed.

Contents of the invention

The purpose of the present invention is to provide a method for prolonging the storage period of the egg mass of the noctuid moth, so as to solve the problem of multi-batch breeding and then eclosion in the above-mentioned background technology. Problems regulating the developmental progression of the egg mass of the wasps of the genus Spodoptera.

In order to achieve the above object, the present invention provides the following technical scheme: a method for prolonging the storage period of the egg mass of Spodoptera spp. , 1-3 parts of inorganic salt, 30-40 parts of sodium alginate, 20-30 parts of carrageenan, 5-10 parts of inorganic antibacterial agent, 40-50 parts of egg white, 3-6 parts of quicklime, 2-4 parts of preservative, 1-3 parts of cellulase, 30-40 parts of sodium alginate, 20-30 parts of carrageenan and 40-50 parts of egg white are installed inside the reaction device. The reaction device is the main outer frame installation structure of the equipment. The top of the reaction device is provided with an extraction box, the interior of the extraction box is provided with a condensation plate, the bottom surface of the reaction device is provided with a motor, the output end of the motor is connected to a rotating shaft, and one end of the rotating shaft runs through the reaction device and a condensation plate, the bottom of the reaction device is provided with a support frame, and the inside of the reaction device is respectively provided with a feed pipe and a discharge pipe.

As a preferred technical solution of the present invention, the outer surface of the shaft is provided with a first paddle, a second paddle and a third paddle sequentially from top to bottom, and the first paddle, the second paddle and the third paddle The outer surfaces of one end of the paddles have the same inclination angle, and the internal structures of the first paddle, the second paddle and the third paddle are the same.

Using the above technical solution, the gap between the first paddle, the second paddle and the third paddle can form a multi-stage vortex when stirring, and one end of the first paddle, the second paddle and the third paddle is from top to bottom. The bottom is gradually inclined, which can guide the materials in a concentrated manner, thereby improving the mixing effect.

As a preferred technical solution of the present invention, the outer surface of the first paddle, the second paddle and the third paddle is provided with a movable shaft, and the outer surface of the movable shaft is annularly distributed with stirring blades, and the number of the stirring blades is the same as that of the first paddle The number of the first paddle board, the second paddle board and the third paddle board is the same.

Using the above technical scheme, when the first paddle, the second paddle and the third paddle are rotating, driven by the water flow, the stirring blades at the bottom of the first paddle, the second paddle and the third paddle will move along the The movable shaft rotates to further improve the mixing reaction effect, and at the same time disrupt the regular stirring inside the equipment and increase the stirring area.

As a preferred technical solution of the present invention, a stirring shaft is arranged symmetrically on the bottom surface of the rotating shaft, one end of the stirring shaft is in an arc shape, a turbine is arranged inside the stirring shaft, and the inside of the stirring shaft is a hollow structure.

With the above technical solution, when the stirring shaft rotates, a vacuum area is generated on the back side, and the negative pressure is used to entrain the water flow, so that the turbine rotates at a high speed, and the raw materials inside the equipment are discharged from the inside of the stirring shaft into the inside of the rotating shaft, increasing the The discharge effect is convenient for the extraction of materials.

As a preferred technical solution of the present invention, through cavities are distributed at equal intervals on the top of the rotating shaft, one end of the through cavity is inclined upward, the inside of the rotating shaft is hollowed out, and the inner surface of the rotating shaft is inclined.

With the above technical solution, the centrifugal force generated by the rotation of the rotating shaft, combined with the shape and structure inside the rotating shaft, makes the material at the bottom of the rotating shaft slide upwards along the inner wall and is discharged from the through cavity. During the discharge process, it is combined with quicklime to avoid the solidification of raw materials , It can also carry out secondary stirring work on the material to improve the overall reaction effect of the raw material.

As a preferred technical solution of the present invention, a material guide frame is provided inside the extraction box, and the material guide frame is in a diamond-shaped structure. A water tank is provided on the top surface of the extraction box, and the water tank and the extraction box are threaded. A discharge channel is opened between the material guide frame and the condensation plate.

With the above technical solution, the material guide frame can not only collect and guide the raw materials thrown out by the rotating shaft according to its own shape and structure, but also guide the water inside the water tank so that an appropriate amount of water can be mixed with the raw materials to increase the amount of raw materials in the raw materials. The dilution is convenient for subsequent shaping work.

As a preferred technical solution of the present invention, fixed frames are distributed at equal intervals inside the extraction box, the perimeter of the fixed frame is smaller than the perimeter of the discharge channel, the fixed frame is connected to the condensation plate, and the bottom of the extraction box A return pipe is provided.

With the above technical scheme, the discharge channel discharges the raw materials into the fixed frame, and the fixed frame absorbs the cooling capacity of the condensing plate, which facilitates the semi-solidification of the raw materials, and the fixed frame is easy to take out from the extraction box, so that the raw materials can be used according to actual needs. applied in different scenarios.

As a preferred technical solution of the present invention, mounting seats are equidistantly distributed on the inner surface of the fixing frame, an extension pole is provided between the mounting seats and the fixing frame, a shaping cylinder is arranged inside the mounting seat, and the fixing The frame and the extraction box are connected by a snug slide.

With the above technical solution, the mounting seat and the extension rod control the position of the shaping cylinder, and multiple fixed frames are placed overlappingly so that the positions of the mounting seats do not overlap each other, so as to avoid uneven distribution of raw materials in the shaping barrel when the raw materials are flowing , thereby increasing the uniformity of material distribution and improving the overall practicability.

The method for prolonging the storage period of the ovum mass of the noctuid moth, comprising the following steps:

S1. Discharge sodium alginate, carrageenan and egg white into the reaction equipment in an appropriate proportion for reaction, and add an appropriate amount of water, stirring at a temperature of 60-80°, and stirring for 30-60 minutes, to obtain egg mass cultivation slurry raw materials.

S2. After step S2 is completed, immediately add quicklime, inorganic antibacterial agent and preservative to the inside of the equipment, speed up the stirring speed, and keep the speed at 12000-15000 rpm.

S3. After the step S3 is completed, the raw materials are discharged into the material guide frame under the centrifugation for static storage, and the thickener, inorganic salt and cellulase are sequentially added to precipitate inside the raw materials.

S4. Lower the temperature inside the extraction box to 2-10 degrees Celsius and let it stand for 10 minutes to obtain semi-solidified material as egg mass placenta.

By adopting the above-mentioned technical scheme, by setting semi-solid raw materials with strong cold absorption to store egg masses, the hatching time of insect eggs can be prolonged in a low-temperature manner, and the raw materials have an antibacterial effect, and at the same time, they have nutrition at one end, which can increase the number of insect eggs. Egg emergence success rate.

Compared with the prior art, the beneficial effect of the present invention is: the method for prolonging the storage period of the egg mass of Noctuid spp.

1. By discharging the raw materials into the fixed frame, the fixed frame absorbs the cooling capacity of the condensation plate, which facilitates the semi-solidification of the raw material, and the fixed frame is easy to take out from the extraction box, so that the raw material can be used in different scenarios according to actual needs At the same time, the raw material after molding is a semi-solid material, and the egg mass can be placed inside. The semi-solid material has good cold absorption, and the combination can maintain the internal low temperature state, prolong the hatching time of the egg mass, and there are business substances in the raw material. Improve the success rate of egg mass eclosion;

2. Through the centrifugal force generated by the rotation of the rotating shaft, combined with the shape and structure inside the rotating shaft, the material at the bottom of the rotating shaft slides upward along the inner wall and is discharged from the through cavity. The material can be stirred twice to improve the overall reaction effect of the raw material. By setting the semi-solid raw material with strong cold absorption to store the egg mass, the hatching time of the eggs can be prolonged at a low temperature, and the raw material has antibacterial properties. Effect.

Description of drawings

Fig. 1 is the schematic view of the internal structure of the reaction device of the present invention;

Fig. 2 is a schematic diagram of the three-dimensional structure of the fixed frame of the present invention;

Fig. 3 is a schematic diagram of the internal front view structure of the rotating shaft of the present invention;

Fig. 4 is a schematic diagram of the internal top view structure of the rotating shaft of the present invention.

In the figure: 1. Reaction device; 2. Support frame; 3. Motor; 4. Rotating shaft; 5. First paddle; 6. Second paddle; 7. Third paddle; 8. Stirring shaft; 9. Turbine ;10, movable shaft; 11, feed pipe; 12, discharge pipe; 13, through cavity; 14, extraction box; 15, condensation plate; 16, material guide frame; 17, water tank; , material return pipe; 20, fixed frame; 21, extension pole; 22, mounting seat; 23, shaping cylinder; 24, stirring blade.

detailed description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Please refer to Fig. 1- Fig. 4, the present invention provides a kind of technical scheme: a kind of prolongation method for the storage period of the ovum mass of Noctuid moth, including material and reaction device 1, material components in parts by weight, thickener 5-10 1-3 parts of inorganic salt, 30-40 parts of sodium alginate, 20-30 parts of carrageenan, 5-10 parts of inorganic antibacterial agent, 40-50 parts of egg white, 3-6 parts of quicklime, 2-4 parts of preservative , 1-3 parts of cellulase, 30-40 parts of sodium alginate, 20-30 parts of carrageenan and 40-50 parts of egg white are installed inside the reaction device 1. The reaction device 1 is the main outer frame installation structure of the equipment, and the reaction device 1 is provided with an extraction box 14, the interior of the extraction box 14 is provided with a condensation plate 15, the bottom surface of the reaction device 1 is provided with a motor 3, the output end of the motor 3 is connected to a rotating shaft 4, and one end of the rotating shaft 4 runs through the reaction device 1 and A cooling plate 15, a support frame 2 is provided at the bottom of the reaction device 1, and a feed pipe 11 and a discharge pipe 12 are respectively provided inside the reaction device 1.

The outer surface of the rotating shaft 4 is sequentially provided with a first paddle 5, a second paddle 6 and a third paddle 7 from top to bottom, and the outer surface of one end of the first paddle 5, the second paddle 6 and the third paddle 7 The angle of inclination is the same, and the internal structure of the first paddle 5, the second paddle 6 and the third paddle 7 is identical; the gap between the first paddle 5, the second paddle 6 and the third paddle 7, when stirring, can A multi-stage vortex is formed, and one end of the first paddle 5, the second paddle 6, and the third paddle 7 is gradually inclined from top to bottom, which can guide the materials in a concentrated manner, thereby improving the mixing effect.

The outer surface of the first paddle 5, the second paddle 6 and the third paddle 7 is provided with a movable shaft 10, and the outer surface of the movable shaft 10 is annularly distributed with stirring blades 24, and the number of the stirring blades 24 is the same as that of the first paddle 5, the second The number of paddle boards 6 and the third paddle boards 7 is the same; the first paddle board 5, the second paddle board 6 and the third paddle board 7 are driven by the water flow to make the first paddle board 5 and the second paddle board 6 and the stirring blade 24 at the bottom of the third paddle 7 rotates along the movable shaft 10, further improving the mixing reaction effect, disrupting the regular stirring inside the equipment and increasing the stirring area.

The bottom surface of the rotating shaft 4 is symmetrically provided with a stirring shaft 8, and one end of the stirring shaft 8 is in an arc-shaped structure. The inside of the stirring shaft 8 is provided with a turbine 9, and the inside of the stirring shaft 8 is a hollow structure. When the stirring shaft 8 rotates, the back side generates a vacuum area, and use the negative pressure to entrain the water flow, so that the turbine 9 rotates at a high speed, and the raw materials inside the equipment are discharged from the inside of the stirring shaft 8 to the inside of the rotating shaft 4, which increases the discharge effect and facilitates the extraction of materials.

There are through cavities 13 distributed at equal intervals on the top of the rotating shaft 4. One end of the through cavity 13 is inclined upward. The inside of the rotating shaft 4 is a hollow structure, and the inner surface of the rotating shaft 4 is inclined. The shape structure makes the material at the bottom of the rotating shaft 4 slide upward along the inner wall and is discharged from the through cavity 13. During the discharge process, quicklime is used to avoid the solidification of the raw material, and the material can also be stirred twice to improve the overall stability of the raw material. Reaction effect.

The inside of the extraction box 14 is provided with a material guide frame 16, the material guide frame 16 is a diamond-shaped structure, and the top surface of the extraction box 14 is provided with a water tank 17. There is a discharge channel 18; according to its own shape and structure, the material guide frame 16 can not only collect and guide the raw materials thrown out by the rotating shaft 4, but also guide the water inside the water tank 17, so that an appropriate amount of water can be mixed with the raw materials , to increase the dilution in the raw material, which is convenient for the subsequent shaping work.

The interior of the extraction box 14 is equidistantly distributed with fixed frames 20, the perimeter of the fixed frame 20 is less than the perimeter of the discharge channel 18, the fixed frame 20 is connected to the condensation plate 15, and the bottom of the extraction box 14 is provided with a return pipe 19; The channel 18 discharges the raw materials into the fixed frame 20, and the fixed frame 20 absorbs the cooling capacity of the condensation plate 15, which facilitates the semi-solidification of the raw materials, and the fixed frame 20 is convenient to take out from the extraction box 14, so that the raw materials can be used according to actual needs in different scenarios.

Mounting seats 22 are distributed at equal intervals on the inner surface of the fixed frame 20, and an extension pole 21 is provided between the mounting seats 22 and the fixed frame 20. A plastic tube 23 is arranged inside the mounting seat 22, and the fixed frame 20 and the extraction box 14 are bonded together. Sliding connection; the mounting seat 22 and the extension rod 21 control the position of the shaping tube 23, and a plurality of fixed frames 20 are stacked so that the positions of the mounting seats 22 do not overlap each other, so as to avoid the The distribution of raw materials is uneven, which increases the uniformity of material distribution and improves the overall practicability.

Working principle: When using the method for extending the storage period of the egg mass of the noctuid moth, the prepared materials are first put into the reaction device 1 in sequence according to the proportion, and then the motor 3 is started to rotate the rotating shaft 4, and the first paddle plate 5, The second paddle 6 and the third paddle 7 stir the raw materials. During the stirring process, cooperate with the movable shaft 10 and the stirring blade 24 to stir the materials step by step to improve the stirring effect, and the raw materials inside the equipment are sucked by the turbine 9 To the inside of the rotating shaft 4, under the action of high-speed centrifugation, the raw material slides upward along the inner surface of the rotating shaft 4, and is thrown out from the inside of the through cavity 13, and then the clean water in the water tank 17 flows out evenly along the inner wall of the material guide frame 16, increasing The dilution of the raw material is finally discharged from the discharge channel 18 into the interior of the fixed frame 20, and accumulated in the interior of the shaping cylinder 23 to wait for cooling and forming. The formed raw material is a semi-solid material, and the eggs can be placed inside. The semi-solid material It has good cold absorption, and can maintain the internal low temperature state with the combination to prolong the hatching time of the egg mass, and the raw material contains commercial substances, which can improve the success rate of the egg mass eclosion, so that the egg mass can be placed in the prefabricated material, according to the material characteristics to achieve control of the eclosion time of egg masses, and improve the storage effect on egg masses of the noctuid moth.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.

Claims

A method for prolonging the storage period of the ovum mass of Spodoptera japonica, characterized in that it includes materials and a reaction device (1), the material components are in parts by weight, 5-10 parts of the thickener, 1-10 parts of inorganic salt 3 parts, sodium alginate 30-40 parts, carrageenan 20-30 parts, inorganic antibacterial agent 5-10 parts, egg white 40-50 parts, quicklime 3-6 parts, preservative 2-4 parts, cellulase 1- 3 parts, 30-40 parts of sodium alginate, 20-30 parts of carrageenan and 40-50 parts of egg white are housed inside the reaction device (1), and the reaction device (1) is the main outer frame installation structure of the equipment, The top of the reaction unit (1) is provided with an extraction box (14), the inside of the extraction box (14) is provided with a condensation plate (15), and the bottom surface of the reaction unit (1) is provided with a motor (3) , the output end of the motor (3) is connected with a rotating shaft (4), one end of the rotating shaft (4) runs through the reaction device (1) and the condensation plate (15), and the bottom of the reaction device (1) is provided with a support frame (2), and the inside of the reaction device (1) is respectively provided with a feed pipe (11) and a discharge pipe (12).
A method for prolonging the storage period of the egg mass of Noctuid moth wasp according to claim 1, characterized in that: the outer surface of the rotating shaft (4) is sequentially provided with a first paddle (5) and a second paddle (5) from top to bottom. The paddle (6) and the third paddle (7), the first paddle (5), the second paddle (6) and the third paddle (7) have the same inclination angle on the outer surface of one end, the first paddle The paddle (5), the second paddle (6) and the third paddle (7) have the same inner structure.
A method for prolonging the storage period of the egg mass of Noctuid moth wasp according to claim 2, characterized in that: outside the first paddle (5), the second paddle (6) and the third paddle (7) The surface is provided with a movable shaft (10), and the outer surface of the movable shaft (10) is annularly distributed with stirring blades (24), and the number of the stirring blades (24) is the same as that of the first paddle plate (5) and the second paddle plate (6). ) is identical with the third paddle board (7) quantity.
A method for prolonging the storage period of the egg mass of Noctuid spp. It is an arc-shaped structure, and the inside of the stirring shaft (8) is provided with a turbine (9), and the inside of the stirring shaft (8) is a hollow structure.
A method for prolonging the storage period of the egg mass of Noctuid moth wasp according to claim 1, characterized in that: through cavities (13) are equidistantly distributed on the top of the rotating shaft (4), and one end of the through cavity (13) Inclining upward, the inside of the rotating shaft (4) is a hollow structure, and the inner surface of the rotating shaft (4) is inclined.
A kind of method for prolonging the storage period of the ovum mass of Spodoptera spp. Diamond-shaped structure, the top surface of the extraction box (14) is provided with a water tank (17), the water tank (17) is threaded with the extraction box (14), the material guide frame (16) and the condensation plate (15) A discharge channel (18) is provided between them.
A kind of method for prolonging the storage period of the ovum mass of Noctuid spp. The length is less than the perimeter of the discharge channel (18), the fixed frame (20) is connected to the condensation plate (15), and the bottom of the extraction box (14) is provided with a return pipe (19).
A method for prolonging the storage period of the egg mass of Noctuid moth wasp according to claim 7, characterized in that: the inner surface of the fixed frame (20) is equidistantly distributed with mounting seats (22), and the mounting seats (22) An extension pole (21) is provided between the fixed frame (20), and a plastic tube (23) is provided inside the mounting seat (22), and the fixed frame (20) is attached to the extraction box (14) Swipe to connect.
A kind of method for prolonging the storage period of the ovum mass of Noctuid nigrum according to claim 1, it is characterized in that, comprises the following steps:

S1. Discharge sodium alginate, carrageenan and egg white into the reaction equipment in an appropriate proportion for reaction, and add an appropriate amount of water, stirring at a temperature of 60-80°, and stirring for 30-60 minutes, to obtain egg mass cultivation slurry raw materials.
S2. After step S2 is completed, immediately add quicklime, inorganic antibacterial agent and preservative to the inside of the equipment, speed up the stirring speed, and keep the speed at 12000-15000 rpm.
S3. After the step S3 is completed, the raw materials are discharged into the material guide frame under the centrifugation for static storage, and the thickener, inorganic salt and cellulase are sequentially added to precipitate inside the raw materials.
S4. Lower the temperature inside the extraction box to 2-10 degrees Celsius and let it stand for 10 minutes to obtain semi-solidified material as egg mass placenta.