WO2023214819A1 - Composition for preventing or treating psoriasis comprising isatidis folium extract - Google Patents

Composition for preventing or treating psoriasis comprising isatidis folium extract Download PDF

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WO2023214819A1
WO2023214819A1 PCT/KR2023/006111 KR2023006111W WO2023214819A1 WO 2023214819 A1 WO2023214819 A1 WO 2023214819A1 KR 2023006111 W KR2023006111 W KR 2023006111W WO 2023214819 A1 WO2023214819 A1 WO 2023214819A1
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extract
psoriasis
preventing
composition
present
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PCT/KR2023/006111
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French (fr)
Korean (ko)
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손미원
김신연
최진규
김세웅
김동현
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엠테라파마 주식회사
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Definitions

  • the present invention relates to a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract as an active ingredient.
  • the present invention relates to a food composition, cosmetic composition, or animal feed composition for preventing or improving psoriasis containing the Daecheong leaf extract.
  • Psoriasis is a chronic inflammatory skin disease in which a rash consisting mainly of red papules or plaques of various sizes, covered with silvery-white scales and with clear borders, occurs repeatedly on the skin of the whole body. Histological examination shows epidermal hyperplasia and It is characterized by inflammation of the dermis. As a small, millet-like rash appears on the skin, layers of white, dandruff-like dead skin cells appear on top of it, and as new rashes around it clump together or grow larger, they spread to the surrounding area, covering almost all of the skin on the body with the rash. It usually occurs most often on the knees and elbows, but it also commonly appears on the buttocks, head skin, and other parts of the body, and may be accompanied by itching.
  • T cells which are immune cells in the skin
  • the secreted immune substances stimulate keratinocytes in the skin, causing excessive proliferation and inflammation of keratinocytes.
  • Skin cells grow rapidly and normal differentiation is disrupted, causing dandruff-like keratin to accumulate on the skin. Genetic factors, environmental factors, drugs, skin irritation, dryness, upper respiratory tract inflammation, mental stress, etc. cause this phenomenon. It is known to be a factor that causes or worsens this condition.
  • psoriasis can be accompanied by various complications. Acute and subacute psoriasis may occur, such as systemic pustular psoriasis where pus blisters appear all over the body, or exfoliative psoriasis where dead skin cells fall off. One or more joints may swell and become painful when pressed. Psoriatic arthritis, which causes joint stiffness, may also occur. In addition, it was confirmed that the more severe the psoriasis, the higher the risk of developing cardiovascular diseases such as myocardial infarction, atherosclerosis, pulmonary thrombosis, and stroke, and metabolic syndromes such as high blood pressure, hyperlipidemia, obesity, and diabetes.
  • cardiovascular diseases such as myocardial infarction, atherosclerosis, pulmonary thrombosis, and stroke
  • metabolic syndromes such as high blood pressure, hyperlipidemia, obesity, and diabetes.
  • Treatment of psoriasis includes topical treatment with medication, phototherapy with exposure to light, systemic treatment with medication, and complex treatment that combines two or more of these methods. In mild cases, treatment is usually started with topical medications, and in moderate or severe cases, treatment is performed with phototherapy or oral medications. However, recently, patients with severe psoriasis who do not respond to these treatments or cannot receive these treatments due to side effects are being treated. Biological agent treatment is also used as a target.
  • psoriasis treatments developed to date have the problem of causing infections by simply suppressing the immune system, or causing a rebound phenomenon in which psoriasis worsens rapidly when the drug is discontinued, and have low treatment efficiency.
  • psoriasis is a chronic recurrent disease, there is a need to develop a treatment with fewer side effects and excellent treatment effectiveness.
  • Isatidis Folium is the leaf of Isatis indigotica Fortune, a biennial herb belonging to the Cruciferae family, and is collected in summer and fall.
  • the plant is called Sungram, and the root is called Panramgeun, and it is known to be effective in treating influenza, hepatitis, acute pneumonia, and jaundice.
  • the present inventors made efforts to develop a new treatment with excellent effects on psoriasis using relatively safe natural materials, and as a result, discovered that Daecheong leaf extract can improve psoriasis, and completed the present invention.
  • the purpose of the present invention is to provide a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
  • the present invention aims to provide a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the present invention aims to provide a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the present invention aims to provide a feed composition for animals for preventing or improving psoriasis, including a Daecheong leaf extract.
  • the present invention provides a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
  • the present invention provides a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the present invention provides a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the present invention provides a feed composition for animals for preventing or improving psoriasis, comprising a Daecheong leaf extract.
  • the extract may be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, and mixed solvents thereof, preferably 0.01% to 90% ethanol extract, more preferably 60% to 60%. It may be an 80% ethanol extract, most preferably a 70% ethanol extract.
  • the present invention provides a composition for the prevention, improvement or treatment of psoriasis, comprising extract of cod leaf extract.
  • the present invention provides the use of a composition containing a cocoa leaf extract for preventing, improving or treating psoriasis.
  • the present invention provides a method for preventing, improving, or treating psoriasis by administering a composition containing a cocoa leaf extract to a subject with psoriasis disease.
  • the composition containing the Daecheong leaf extract of the present invention as an active ingredient has an excellent therapeutic effect on psoriasis and can be used as a composition for preventing, improving, or treating psoriasis. It is an extract of a natural product, has few side effects, and is safe, preventing psoriasis caused by various causes. It can be very useful for patients with
  • Figure 1 shows the results of confirming the degree of improvement in psoriasis following administration of the extract of the present invention.
  • Figure 2 shows the measurement results of psoriasis severity index (PASI) according to administration of the extract of the present invention.
  • PASI psoriasis severity index
  • the present invention provides a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
  • the Daecheong leaves include dried leaves of Isatis indigotica Fortune.
  • Isatidis Folium of the present invention is the leaf of Isatis indigotica Fortune, a biennial herb belonging to the Cruciferae family. It is collected in summer and fall, and the forerunner is called Sungram and the root is called Panramgeun.
  • the extract used in the present invention can be obtained using a conventional extraction solvent known in the art.
  • a polar solvent or a non-polar solvent can be used.
  • Polar solvents include water, C1 to C6 alcohols (e.g., methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, and n-butanol, etc.), acetic acid, or mixtures of the above polar solvents.
  • Nonpolar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, butyl acetate, fluoroalkane, hexane, ether, chloroform, dichloromethane, or mixtures of the above nonpolar solvents.
  • the extract of the present invention may preferably be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohols, and mixed solvents thereof, preferably 0.01% to 90% ethanol extract, and more preferably May be a 60% to 80% ethanol extract, most preferably a 70% ethanol extract.
  • a solvent selected from the group consisting of water, C1 to C6 alcohols, and mixed solvents thereof, preferably 0.01% to 90% ethanol extract, and more preferably May be a 60% to 80% ethanol extract, most preferably a 70% ethanol extract.
  • the extract used in the present invention may be extracted through hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction, or conventional extraction methods known in the art.
  • extract used in the present invention refers to what is commonly used in the art as a crude extract, but in a broad sense also includes fractions obtained by additional fractionation of the extract. That is, the extract includes not only those obtained using the above-mentioned solvents, but also those obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity, or affinity), etc. Fractions obtained through purification methods are also included in the extract of the present invention.
  • the present invention provides a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the food composition of the present invention may be a health functional food, dairy product, fermented product, or food additive.
  • the above health functional foods refer to foods manufactured and processed using raw materials or ingredients with functional properties useful to the human body. “Functional” refers to foods that are useful for health purposes such as regulating nutrients for the structure and function of the human body or physiological effects. It means taking it for the purpose of obtaining effects.
  • the present invention provides a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
  • the present invention provides a feed composition for animals for preventing or improving psoriasis, comprising a Daecheong leaf extract.
  • the animal feed composition of the present invention can be ingested by all non-human animals, such as non-human primates, sheep, dogs, cows, horses, etc.
  • prevention refers to all actions that suppress or delay a disease, etc. by administration of the composition according to the present invention
  • improvement refers to parameters related to the condition treated by administration of the composition of the present invention, For example, it refers to any action that at least reduces the degree of symptoms
  • treatment refers to any action that improves or beneficially changes the symptoms of an individual suspected or affected by a disease by administering the composition of the present invention.
  • Washed and dried Daecheong leaves (Isatidis Folium) were pulverized according to herbal medicine and used.
  • a 10-fold 70% ethanol aqueous solution was added to the Daecheong leaves and extracted for 72 hours at room temperature. After filtering the extract, it was concentrated under reduced pressure at 40 to 60°C to obtain a powdered herbal extract.
  • Psoriasis Severity Index (PASI) evaluation according to extract administration
  • PASI stands for ‘Psoriasis Severity Index’ and is a type of composite index that combines the psoriasis lesion involvement area (BSA) and the severity of the lesion. It is used to evaluate the efficacy of treatment as an index to measure the severity of the psoriasis affected area by calculating the distribution range of the three characteristics of psoriasis: Erythema (skin redness), scaling (skin keratinization), and ear thickness. (Refer to Table 2 below).
  • Psoriasis Area Severity Index (PASI) scoring (range 0 to 9) Erythema score Scaling score Ear Thickness score no redness, pale pink 0 0% 0 ⁇ 0.25mm 0 pale pink with ⁇ 20% red patches One ⁇ 20% One 0.25-0.3mm One pink back with 20-60% red patches 2 20-60% 2 0.3-0.35mm 2 pink/red back with >60% red patches 3 >60% 3 0.35mm ⁇ 3
  • the Daecheong leaf extract of the present invention has an excellent psoriasis treatment effect.

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Abstract

The present invention relates to a composition for the prevention, amelioration or treatment of psoriasis comprising Isatidis Folium extract as an active ingredient, which is an extract of a natural product and is safe with few side effects and can be very useful for patients with psoriasis caused by various causes.

Description

대청엽 추출물을 포함하는 건선의 예방 또는 치료용 조성물Composition for preventing or treating psoriasis containing Daecheong leaf extract
본 발명은 대청엽 추출물을 유효성분으로 포함하는 건선의 예방 또는 치료용 약학 조성물에 관한 것이다. 또한, 본 발명은 상기 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 식품 조성물, 화장료 조성물 또는 동물용 사료 조성물에 관한 것이다.The present invention relates to a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract as an active ingredient. In addition, the present invention relates to a food composition, cosmetic composition, or animal feed composition for preventing or improving psoriasis containing the Daecheong leaf extract.
건선(psoriasis)은 은백색의 비늘로 덮여 있고, 경계가 뚜렷하며 크기가 다양한 붉은색의 구진이나 판으로 주로 구성된 발진이 전신의 피부에 반복적으로 발생하는 만성 염증성 피부질환으로, 조직 검사상 표피의 증식과 진피의 염증을 특징으로 한다. 피부에 작은 좁쌀 같은 발진이 생기면서 그 위로 새하얀 비듬 같은 각질이 겹겹이 쌓여 나타나게 되고, 주위에서 발생한 새로운 발진들과 서로 뭉쳐지거나 커지면서 주위로 퍼져 나가 전신의 거의 모든 피부가 발진으로 덮이기도 한다. 통상적으로 무릎과 팔꿈치에 가장 많이 생기나 엉덩이나 머리 피부 및 몸의 다른 부위에도 흔히 나타나며, 가려움증이 동반되기도 한다.Psoriasis is a chronic inflammatory skin disease in which a rash consisting mainly of red papules or plaques of various sizes, covered with silvery-white scales and with clear borders, occurs repeatedly on the skin of the whole body. Histological examination shows epidermal hyperplasia and It is characterized by inflammation of the dermis. As a small, millet-like rash appears on the skin, layers of white, dandruff-like dead skin cells appear on top of it, and as new rashes around it clump together or grow larger, they spread to the surrounding area, covering almost all of the skin on the body with the rash. It usually occurs most often on the knees and elbows, but it also commonly appears on the buttocks, head skin, and other parts of the body, and may be accompanied by itching.
건선의 원인은 아직 완벽하게 밝혀지지 않았지만, 피부에 있는 면역세포인 T세포의 활동성이 증가되어 그 결과 분비된 면역 물질이 피부의 각질세포를 자극하여 각질세포의 과다한 증식과 염증을 일으키는 것으로 보고되고 있다. 피부 세포가 빠르게 자라나고 정상적인 분화에 장애가 발생되어 피부 위에 비듬 같은 각질이 겹겹이 쌓여서 나타나게 되고, 이러한 현상이 발생됨에 있어서 유전적 요인, 환경적 요인, 약물, 피부자극, 건조, 상기도 염증, 정신적 스트레스 등이 원인이 되거나 악화시키는 요인으로 알려져 있다.Although the cause of psoriasis is not fully known yet, it is reported that the activity of T cells, which are immune cells in the skin, increases, and as a result, the secreted immune substances stimulate keratinocytes in the skin, causing excessive proliferation and inflammation of keratinocytes. there is. Skin cells grow rapidly and normal differentiation is disrupted, causing dandruff-like keratin to accumulate on the skin. Genetic factors, environmental factors, drugs, skin irritation, dryness, upper respiratory tract inflammation, mental stress, etc. cause this phenomenon. It is known to be a factor that causes or worsens this condition.
또한, 건선은 여러 가지 합병증을 동반할 수 있다. 몸 전체에 고름물집이 생기는 전신성 농포성 건선이나 각질이 떨어져나가는 박탈성 건선과 같은 급성(acute) 건선과 아급성(subacute) 건선이 발생하기도 하고, 관절 부위가 하나 이상 부어오르면서 누르면 아프기도 하고 관절이 뻣뻣해지는 건선 관절염이 발생하기도 한다. 또한, 건선이 심할수록 심근경색, 죽경화증, 폐혈전증, 뇌졸중과 같은 심혈관계질환이나 고혈압, 고지혈증, 비만, 당뇨병과 같은 대사증후군의 발생위험률이 높다는 것이 확인되었다.Additionally, psoriasis can be accompanied by various complications. Acute and subacute psoriasis may occur, such as systemic pustular psoriasis where pus blisters appear all over the body, or exfoliative psoriasis where dead skin cells fall off. One or more joints may swell and become painful when pressed. Psoriatic arthritis, which causes joint stiffness, may also occur. In addition, it was confirmed that the more severe the psoriasis, the higher the risk of developing cardiovascular diseases such as myocardial infarction, atherosclerosis, pulmonary thrombosis, and stroke, and metabolic syndromes such as high blood pressure, hyperlipidemia, obesity, and diabetes.
이러한 건선의 치료에는 약을 바르는 국소치료, 광선을 쪼이는 광치료, 약을 먹는 전신치료, 그리고 이러한 방법을 두 가지 이상 복합한 복합 치료 등이 있다. 가벼운 경우에는 대개 바르는 약으로 치료를 시작하고 중등증이나 중증이 되면 광치료나 먹는 약으로 치료를 하는 것이 일반적이나, 최근에는 이러한 치료에 반응이 없거나 부작용 등으로 이러한 치료를 받을 수 없는 중증 건선 환자를 대상으로 생물학적 제제 치료가 사용되기도 한다.Treatment of psoriasis includes topical treatment with medication, phototherapy with exposure to light, systemic treatment with medication, and complex treatment that combines two or more of these methods. In mild cases, treatment is usually started with topical medications, and in moderate or severe cases, treatment is performed with phototherapy or oral medications. However, recently, patients with severe psoriasis who do not respond to these treatments or cannot receive these treatments due to side effects are being treated. Biological agent treatment is also used as a target.
그러나 현재까지 개발된 건선 치료제는 단순히 면역을 억제함으로써 감염증을 유발하거나 약물을 중단하는 경우 건선이 급격히 악화되는 반동현상이 일어날 수 있으며, 치료 효율이 낮은 문제점이 있다. 또한 건선은 만성 재발성 질환이므로 부작용이 적고 치료 효과가 우수한 치료제의 개발이 필요한 실정이다.However, psoriasis treatments developed to date have the problem of causing infections by simply suppressing the immune system, or causing a rebound phenomenon in which psoriasis worsens rapidly when the drug is discontinued, and have low treatment efficiency. In addition, since psoriasis is a chronic recurrent disease, there is a need to develop a treatment with fewer side effects and excellent treatment effectiveness.
한편, 대청엽(Isatidis Folium)은 십자화과(Cruciferae)에 속한 이년생 초본인 숭람(Isatis indigotica Fortune)의 잎으로, 여름과 가을에 채취한다. 전초는 숭람, 뿌리는 판람근이라 하며, 유행성감기, 간염, 급성폐렴, 황달 등에 효능이 있는 것으로 알려져 있다.Meanwhile, Isatidis Folium is the leaf of Isatis indigotica Fortune, a biennial herb belonging to the Cruciferae family, and is collected in summer and fall. The plant is called Sungram, and the root is called Panramgeun, and it is known to be effective in treating influenza, hepatitis, acute pneumonia, and jaundice.
이에, 본 발명자들은 비교적 안전한 천연물 유래 소재를 이용한 건선에 대한 우수한 효과를 갖는 신규한 치료제를 개발하기 위해 노력한 결과, 대청엽 추출물이 건선을 개선시킬 수 있는 것을 발견하여, 본 발명을 완성하였다.Accordingly, the present inventors made efforts to develop a new treatment with excellent effects on psoriasis using relatively safe natural materials, and as a result, discovered that Daecheong leaf extract can improve psoriasis, and completed the present invention.
본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 치료용 약학 조성물을 제공하는 것을 목적으로 한다.The purpose of the present invention is to provide a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 식품 조성물을 제공하는 것을 목적으로 한다.Additionally, the present invention aims to provide a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 화장료 조성물을 제공하는 것을 목적으로 한다.In addition, the present invention aims to provide a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 동물용 사료 조성물을 제공하는 것을 목적으로 한다.In addition, the present invention aims to provide a feed composition for animals for preventing or improving psoriasis, including a Daecheong leaf extract.
본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 치료용 약학 조성물을 제공한다.The present invention provides a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 식품 조성물을 제공한다.In addition, the present invention provides a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 화장료 조성물을 제공한다.In addition, the present invention provides a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 동물용 사료 조성물을 제공한다.In addition, the present invention provides a feed composition for animals for preventing or improving psoriasis, comprising a Daecheong leaf extract.
상기 추출물은 물, C1 내지 C6의 알코올, 및 이들의 혼합 용매로 이루어지는 군으로부터 선택되는 용매로 추출된 것일 수 있으며, 바람직하게 0.01% 내지 90% 에탄올 추출물일 수 있으며, 더욱 바람직하게는 60% 내지 80% 에탄올 추출물일 수 있고, 가장 바람직하게는 70% 에탄올 추출물일 수 있다.The extract may be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, and mixed solvents thereof, preferably 0.01% to 90% ethanol extract, more preferably 60% to 60%. It may be an 80% ethanol extract, most preferably a 70% ethanol extract.
본 발명은 대청엽 추출물을 포함하는 건선의 예방, 개선 또는 치료 용도를 위한 조성물을 제공한다.The present invention provides a composition for the prevention, improvement or treatment of psoriasis, comprising extract of cod leaf extract.
본 발명은 대청엽 추출물을 포함하는 건선의 예방, 개선 또는 치료를 위한 조성물의 용도를 제공한다.The present invention provides the use of a composition containing a cocoa leaf extract for preventing, improving or treating psoriasis.
본 발명은 대청엽 추출물을 포함하는 조성물을 건선 질환을 갖는 대상체에 투여하여 건선의 예방, 개선 또는 치료하는 방법을 제공한다.The present invention provides a method for preventing, improving, or treating psoriasis by administering a composition containing a cocoa leaf extract to a subject with psoriasis disease.
본 발명의 대청엽 추출물을 유효성분으로 포함하는 조성물은 건선에 대하여 우수한 치료 효과를 나타내어 건선의 예방, 개선 또는 치료용 조성물로 사용될 수 있으며, 천연물의 추출물로 부작용이 적고 안전하여 다양한 원인으로 인한 건선을 갖는 환자들에게 매우 유용하게 사용될 수 있다.The composition containing the Daecheong leaf extract of the present invention as an active ingredient has an excellent therapeutic effect on psoriasis and can be used as a composition for preventing, improving, or treating psoriasis. It is an extract of a natural product, has few side effects, and is safe, preventing psoriasis caused by various causes. It can be very useful for patients with
도 1은 본 발명의 추출물 투여에 따른 건선 개선 정도를 확인한 결과를 나타낸다.Figure 1 shows the results of confirming the degree of improvement in psoriasis following administration of the extract of the present invention.
도 2는 본 발명의 추출물 투여에 따른 건선 중증도 지수(PASI)의 측정 결과를 나타낸다.Figure 2 shows the measurement results of psoriasis severity index (PASI) according to administration of the extract of the present invention.
이하, 첨부한 도면을 참조하여 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본원의 실시태양 및 실시예를 상세히 설명한다. 그러나 본원은 여러 가지 형태로 구현될 수 있으며 여기에서 설명하는 실시태양 및 실시예에 한정되지 않는다.Hereinafter, with reference to the attached drawings, embodiments and examples of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present application may be implemented in various forms and is not limited to the embodiments and examples described herein.
본원 명세서 전체에서, 어떤 부분이 어떤 구성 요소를 "포함" 한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성 요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.Throughout the specification of the present application, when a part "includes" a certain component, this means that it may further include other components rather than excluding other components unless specifically stated to the contrary.
본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 치료용 약학 조성물을 제공한다.The present invention provides a pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaf extract.
상기 대청엽은 숭람(Isatis indigotica Fortune)의 잎을 건조한 것을 포함한다.The Daecheong leaves include dried leaves of Isatis indigotica Fortune.
본 발명의 대청엽(Isatidis Folium)은 십자화과(Cruciferae)에 속한 이년생 초본인 숭람(Isatis indigotica Fortune)의 잎으로, 여름과 가을에 채취하고, 전초는 숭람, 뿌리는 판람근이라 한다.Isatidis Folium of the present invention is the leaf of Isatis indigotica Fortune, a biennial herb belonging to the Cruciferae family. It is collected in summer and fall, and the forerunner is called Sungram and the root is called Panramgeun.
본 발명에 사용되는 추출물은 당업계에서 공지된 통상적인 추출용매를 사용하여 얻을 수 있다. 추출용매로는 극성 용매 또는 비극성 용매를 이용할 수 있다. 극성 용매로는 물, C1 내지 C6의 알코올(예: 메탄올, 에탄올, 프로판올, 부탄올, 노말-프로판올, 이소-프로판올 및 노말-부탄올 등), 아세트산, 또는 상기 극성 용매들의 혼합물을 포함한다. 비극성 용매로는 아세톤, 아세토나이트릴, 에틸아세테이트, 메틸아세테이트, 부틸아세테이트, 플루오로알칸, 헥산, 에테르, 클로로포름, 디클로로메탄 또는 상기 비극성 용매들의 혼합물을 포함한다.The extract used in the present invention can be obtained using a conventional extraction solvent known in the art. As the extraction solvent, a polar solvent or a non-polar solvent can be used. Polar solvents include water, C1 to C6 alcohols (e.g., methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, and n-butanol, etc.), acetic acid, or mixtures of the above polar solvents. Nonpolar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, butyl acetate, fluoroalkane, hexane, ether, chloroform, dichloromethane, or mixtures of the above nonpolar solvents.
본 발명의 추출물은 바람직하게 물, C1 내지 C6의 알코올, 및 이들의 혼합 용매로 이루어지는 군으로부터 선택되는 용매로 추출될 수 있고, 바람직하게는 0.01% 내지 90% 에탄올 추출물일 수 있으며, 더욱 바람직하게는 60% 내지 80% 에탄올 추출물일 수 있고, 가장 바람직하게는 70% 에탄올 추출물일 수 있다.The extract of the present invention may preferably be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohols, and mixed solvents thereof, preferably 0.01% to 90% ethanol extract, and more preferably May be a 60% to 80% ethanol extract, most preferably a 70% ethanol extract.
본 발명에 사용되는 추출물은 열수 추출, 냉침 추출, 환류 냉각 추출, 초음파 추출 또는 당업계에 알려진 통상적인 추출방법을 통해 추출한 것일 수 있다.The extract used in the present invention may be extracted through hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction, or conventional extraction methods known in the art.
본 발명에 사용된 용어 “추출물”은 당업계에서 조추출물(crude extract)로 통용되는 것을 의미하지만, 광의적으로 추출물을 추가적으로 분획(fractionation)한 분획물도 포함한다. 즉 추출물은 상술한 용매를 이용하여 얻은 것뿐만 아니라, 여기에 정제 과정을 추가적으로 적용하여 얻은 것을 포함한다. 예컨대, 상기 추출물을 일정한 분자량 컷-오프 값을 갖는 한외여과막을 통과시켜 얻은 분획, 다양한 크로마토그래프(크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 추출물에 포함된다.The term “extract” used in the present invention refers to what is commonly used in the art as a crude extract, but in a broad sense also includes fractions obtained by additional fractionation of the extract. That is, the extract includes not only those obtained using the above-mentioned solvents, but also those obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity, or affinity), etc. Fractions obtained through purification methods are also included in the extract of the present invention.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 식품 조성물을 제공한다.In addition, the present invention provides a food composition for preventing or improving psoriasis containing Daecheong leaf extract.
본 발명의 식품 조성물은 건강기능식품, 유제품, 발효제품 또는 식품 첨가물일 수 있다.The food composition of the present invention may be a health functional food, dairy product, fermented product, or food additive.
상기 건강기능식품은 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미하며, “기능성”은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻을 목적으로 섭취하는 것을 의미한다.The above health functional foods refer to foods manufactured and processed using raw materials or ingredients with functional properties useful to the human body. “Functional” refers to foods that are useful for health purposes such as regulating nutrients for the structure and function of the human body or physiological effects. It means taking it for the purpose of obtaining effects.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 화장료 조성물을 제공한다.In addition, the present invention provides a cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
또한, 본 발명은 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 동물용 사료 조성물을 제공한다.In addition, the present invention provides a feed composition for animals for preventing or improving psoriasis, comprising a Daecheong leaf extract.
본 발명의 동물용 사료 조성물은 모든 비-인간 동물, 예를 들어 비-인간 영장류, 양, 개, 소, 말 등에게 섭취시킬 수 있다.The animal feed composition of the present invention can be ingested by all non-human animals, such as non-human primates, sheep, dogs, cows, horses, etc.
본 발명에 사용된 용어 “예방”은 본 발명에 따른 조성물의 투여에 의해 질환 등을 억제 또는 지연시키는 모든 행위를 의미하고, “개선”은 본 발명의 조성물의 투여로 치료되는 상태와 관련된 파라미터, 예를 들면 증상의 정도를 적어도 감소시키는 모든 행위를 의미하며, “치료”는 본 발명의 조성물의 투여에 의해 질환의 의심 및 발병 개체의 증상이 호전되거나 이롭게 변경되는 모든 행위를 의미한다.As used in the present invention, the term “prevention” refers to all actions that suppress or delay a disease, etc. by administration of the composition according to the present invention, and “improvement” refers to parameters related to the condition treated by administration of the composition of the present invention, For example, it refers to any action that at least reduces the degree of symptoms, and “treatment” refers to any action that improves or beneficially changes the symptoms of an individual suspected or affected by a disease by administering the composition of the present invention.
이하 실시예를 통하여 본 발명을 더욱 상세하게 설명하고자 하나, 하기의 실시예는 단지 설명의 목적을 위한 것이며 본원 발명의 범위를 한정하고자 하는 것은 아니다.The present invention will be described in more detail through examples below. However, the examples below are for illustrative purposes only and are not intended to limit the scope of the present invention.
[제조예][Manufacturing example]
대청엽 추출물의 제조Preparation of Daecheong leaf extract
세척 및 건조된 대청엽(Isatidis Folium)을 생약에 따라 분쇄하여 사용하였다. 상기 대청엽에 10배의 70% 에탄올 수용액을 가하여 상온에서 72시간 동안 추출하였다. 추출액을 여과한 후, 40 ~ 60 ℃에서 감압 농축하여 분말 상태의 생약 추출물을 수득하였다.Washed and dried Daecheong leaves (Isatidis Folium) were pulverized according to herbal medicine and used. A 10-fold 70% ethanol aqueous solution was added to the Daecheong leaves and extracted for 72 hours at room temperature. After filtering the extract, it was concentrated under reduced pressure at 40 to 60°C to obtain a powdered herbal extract.
[실시예 1][Example 1]
추출물 투여에 따른 건선 중증도 지수(PASI) 평가Psoriasis Severity Index (PASI) evaluation according to extract administration
상기 제조예를 통해 제조된 대청엽 추출물의 건선의 치료 효능을 평가하기 위하여, 6주령의 BALB/cAnNCrlOri 수컷 마우스를 1주일간 예비사육한 후 하기 표 1에 나타낸 바와 같이 6마리씩 나누어 실험을 진행하였다.In order to evaluate the psoriasis treatment efficacy of the Daecheong leaf extract prepared through the above preparation example, 6-week-old BALB/cAnNCrlOri male mice were preliminarily reared for 1 week, and then the experiment was conducted by dividing them into 6 mice as shown in Table 1 below.
정상군Jeongsang-gun --
음성대조군Negative control group Aldara™ 5% cream imiquimod (IMQ) 62.5mg 피부 도포Aldara™ 5% cream imiquimod (IMQ) 62.5mg dermal application
양성대조군Positive control group Aldara™ 5% cream imiquimod (IMQ) 62.5mg 피부 도포 + Methotrexate 1mg/kgAldara™ 5% cream imiquimod (IMQ) 62.5mg dermal application + Methotrexate 1mg/kg
실험군experimental group Aldara™ 5% cream imiquimod (IMQ) 62.5mg 피부 도포 + 대청엽 추출물 100mg/kgAldara™ 5% cream imiquimod (IMQ) 62.5mg dermal application + cod extract 100mg/kg
실험 시작 하루 전에 각 군 마우스의 등 및 엉덩이 부위에 제모크림을 이용하여 제모하고 1일 후 이미퀴모드 5% 크림(Aldara™ 5% cream imiquimod (IMQ), 동아에스티)을 매일 62.5mg/mice씩 등에 8일간 도포하였다. 상기 동물 모델에 메토트렉세이트(methotrexate) 1mg/kg 및 대청엽 추출물 100mg/kg을 매일 경구투여한 후 건선 개선 정도를 육안으로 측정하고, 건선의 중증도 지수(PASI(Psoriasis Area and Severity Index)) 스코어를 측정하여 각 군의 건선 중증도 지수를 평가하였다.One day before the start of the experiment, hair was removed using hair removal cream on the back and buttocks of each group of mice, and one day later, 62.5 mg/mice of imiquimod 5% cream (Aldara™ 5% cream imiquimod (IMQ), Dong-A ST) was administered daily. It was applied to the back for 8 days. After daily oral administration of 1 mg/kg of methotrexate and 100 mg/kg of Daecheong leaf extract to the animal model, the degree of improvement in psoriasis was visually measured, and the psoriasis severity index (PASI (Psoriasis Area and Severity Index)) score was measured. The psoriasis severity index of each group was then evaluated.
PASI는 ‘건선 중증도 지수’의 약자로 건선 병변의 침범 면적(BSA)과 병변의 중증도를 결합시킨 일종의 복합지수이다. 건선의 3가지 특징인 병변의 Erythema(피부발적), Scaling(피부각질화), 두께(Ear thickness)의 분포 범위를 계산하여 건선 침범 부위의 심각성 정도를 측정하는 지표로 치료제의 효능을 평가하는 데 사용된다(하기 표 2 참고).PASI stands for ‘Psoriasis Severity Index’ and is a type of composite index that combines the psoriasis lesion involvement area (BSA) and the severity of the lesion. It is used to evaluate the efficacy of treatment as an index to measure the severity of the psoriasis affected area by calculating the distribution range of the three characteristics of psoriasis: Erythema (skin redness), scaling (skin keratinization), and ear thickness. (Refer to Table 2 below).
Psoriasis Area Severity Index (PASI) scoring (range 0 to 9)Psoriasis Area Severity Index (PASI) scoring (range 0 to 9)
ErythemaErythema 점수score ScalingScaling 점수score Ear ThicknessEar Thickness 점수score
no redness, pale pinkno redness, pale pink 00 0%0% 00 <0.25mm<0.25mm 00
pale pink with <20% red patchespale pink with <20% red patches 1One <20%<20% 1One 0.25-0.3mm0.25-0.3mm 1One
pink back with 20-60% red patchespink back with 20-60% red patches 22 20-60%20-60% 22 0.3-0.35mm0.3-0.35mm 22
pink/red back with >60% red patchespink/red back with >60% red patches 33 >60%>60% 33 0.35mm<0.35mm< 33
그 결과, 도 1 및 2에 나타낸 바와 같이, 이미퀴모드 도포 8일째, 정상군(NC)과 비교하여 음성대조군에서는 현저히 피부 병변의 홍반(Erythema), 각질(Scaling), 두꺼워짐(Ear thickness)이 증가하여, 건선의 임상적 변화를 나타내었다(PASI score: 3.11). 반면, 양성대조군(PASI score: 1.94) 및 실험군(PASI score: 2.0)에서는 피부 병변의 홍반, 각질, 두꺼워짐이 감소하는 것으로 나타났고, 음성대조군과 비교하여 건선 중증도 지수(PASI)가 현저하게 감소되는 것을 확인하였다.As a result, as shown in Figures 1 and 2, on the 8th day of application of imiquimod, erythema, scaling, and thickening of skin lesions were significantly observed in the negative control group compared to the normal group (NC). This increased, indicating clinical changes in psoriasis (PASI score: 3.11). On the other hand, the positive control group (PASI score: 1.94) and the experimental group (PASI score: 2.0) showed a decrease in erythema, keratinization, and thickening of skin lesions, and the Psoriasis Severity Index (PASI) significantly decreased compared to the negative control group. It was confirmed that this was the case.
따라서, 본 발명의 대청엽 추출물은 우수한 건선 치료 효과를 갖는 것을 알 수 있다.Therefore, it can be seen that the Daecheong leaf extract of the present invention has an excellent psoriasis treatment effect.

Claims (8)

  1. 대청엽 추출물을 포함하는 건선의 예방 또는 치료용 약학 조성물.A pharmaceutical composition for preventing or treating psoriasis containing extract of Daecheong leaves.
  2. 제1항에 있어서, 상기 추출물은 물, C1 내지 C6의 알코올, 및 이들의 혼합 용매로 이루어지는 군으로부터 선택되는 용매로 추출되는 것을 특징으로 하는, 건선의 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating psoriasis according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, and mixed solvents thereof.
  3. 제1항에 있어서, 상기 추출물은 0.01% 내지 90% 에탄올 추출물인 것을 특징으로 하는, 건선의 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating psoriasis according to claim 1, wherein the extract is a 0.01% to 90% ethanol extract.
  4. 제1항에 있어서, 상기 추출물은 60% 내지 80% 에탄올 추출물인 것을 특징으로 하는, 건선의 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating psoriasis according to claim 1, wherein the extract is a 60% to 80% ethanol extract.
  5. 제1항에 있어서, 상기 추출물은 70% 에탄올 추출물인 것을 특징으로 하는, 건선의 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating psoriasis according to claim 1, wherein the extract is a 70% ethanol extract.
  6. 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 식품 조성물.A food composition for preventing or improving psoriasis containing Daecheong leaf extract.
  7. 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 화장료 조성물.A cosmetic composition for preventing or improving psoriasis containing Daecheong leaf extract.
  8. 대청엽 추출물을 포함하는 건선의 예방 또는 개선용 동물용 사료 조성물.An animal feed composition for preventing or improving psoriasis containing extract of Daecheong leaf.
PCT/KR2023/006111 2022-05-04 2023-05-04 Composition for preventing or treating psoriasis comprising isatidis folium extract WO2023214819A1 (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050004354A (en) * 2003-07-02 2005-01-12 나드리화장품주식회사 Cosmetics composition containing herb medicine extracts and perfumes for preventing acne or inflammation
KR101191724B1 (en) * 2010-10-07 2012-10-16 재단법인 한국한방산업진흥원 Composition comprising a leaf extract of Isatis indigotica Fort showing skin whitening and anti-wrinkle activity
CN103705664A (en) * 2012-10-09 2014-04-09 黑龙江省中医研究院 Pharmaceutical composition used for treating psoriasis, and preparation method thereof
CN107929669A (en) * 2017-12-11 2018-04-20 汤阴县瑞星生物有限公司 A kind of Chinese medicine composition for treating psoriasis and preparation method thereof
CN111035668A (en) * 2019-12-11 2020-04-21 中国科学院新疆理化技术研究所 Preparation method and application of instant isatis tinctoria leaf extract

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050004354A (en) * 2003-07-02 2005-01-12 나드리화장품주식회사 Cosmetics composition containing herb medicine extracts and perfumes for preventing acne or inflammation
KR101191724B1 (en) * 2010-10-07 2012-10-16 재단법인 한국한방산업진흥원 Composition comprising a leaf extract of Isatis indigotica Fort showing skin whitening and anti-wrinkle activity
CN103705664A (en) * 2012-10-09 2014-04-09 黑龙江省中医研究院 Pharmaceutical composition used for treating psoriasis, and preparation method thereof
CN107929669A (en) * 2017-12-11 2018-04-20 汤阴县瑞星生物有限公司 A kind of Chinese medicine composition for treating psoriasis and preparation method thereof
CN111035668A (en) * 2019-12-11 2020-04-21 中国科学院新疆理化技术研究所 Preparation method and application of instant isatis tinctoria leaf extract

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