WO2023208030A1 - 一种诱导单面叶片光合作用装置 - Google Patents

一种诱导单面叶片光合作用装置 Download PDF

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Publication number
WO2023208030A1
WO2023208030A1 PCT/CN2023/090803 CN2023090803W WO2023208030A1 WO 2023208030 A1 WO2023208030 A1 WO 2023208030A1 CN 2023090803 W CN2023090803 W CN 2023090803W WO 2023208030 A1 WO2023208030 A1 WO 2023208030A1
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carbon dioxide
leaf
chamber
external circulation
organic
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PCT/CN2023/090803
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English (en)
French (fr)
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常硕其
石丹丹
欧阳翔
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湖南杂交水稻研究中心
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Publication of WO2023208030A1 publication Critical patent/WO2023208030A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098—Plants or trees
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004—Gaseous mixtures, e.g. polluted air
    • G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
    • G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
    • G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
    • G01N33/004—CO or CO2

Definitions

  • the Chinese patent application with publication number CN203981676U discloses a device and method for rapid measurement of photosynthetic rate of whole plant tissue culture seedlings, including an IRGA sample chamber of a plant photosynthetic meter, a soil leaf chamber mounting plate, an IRGA sample chamber and a soil leaf chamber mounting plate. It forms a complex and is also equipped with a transparent self-made leaf chamber.
  • the self-made leaf chamber is equipped with fixed holes, pores and return pipe interfaces; the self-made leaf chamber is connected to the soil leaf chamber installation plate through the fixed holes, and the self-made leaf chamber is connected to the complex. There is a rigid sealed connection between them; the IRGA sample chamber is connected to the photosynthesizer host through a data line.
  • the photosynthetic meter can intuitively display the photosynthetic rate of the plant in real time without any mechanical pressure damage, and can arbitrarily It can measure the photosynthetic rate of a single plant or a whole bottle (group) of tissue culture seedlings; it also solves the problem that the standard leaf chamber cannot measure a single leaf with an area less than 6cm 2 , but it still cannot measure the photosynthesis of a single leaf of a certain plant. Determination.
  • the Chinese patent application with publication number CN205080115U discloses a plant synthesis rate measuring device, which includes an LED lamp to provide a plant light source and can adjust the light intensity changes; an air pump to transport CO 2 gas; a leaf chamber to fix the measuring blades; and a flow meter to detect CO 2 gas flow; CO 2 analyzer, detects CO 2 concentration; temperature and humidity sensor, detects CO 2 gas temperature and humidity changes; data collector, collects and records the collected data; computer, analyzes the collected data.
  • CO 2 analyzer detects CO 2 concentration
  • temperature and humidity sensor detects CO 2 gas temperature and humidity changes
  • data collector collects and records the collected data
  • computer analyzes the collected data.
  • the Chinese patent application with publication number CN207571108U discloses a device for measuring photosynthetic rate, respiration rate and greenhouse gas emissions at different heights of crop groups, including a test box, a first pole, a second pole, a third pole, 3N Detection component and signal collector, the test box includes a top cover, a box body and a base. A first groove is provided on the top of the box body. A fan is installed on the inner wall of the box. A pair of support brackets are symmetrically installed on the inner wall of the box.
  • the first Both ends of the support pole, the second support pole, and the third support pole are detachably fixed on the two support frames respectively, and the first support pole, the second support pole, and the third support pole are equally spaced in the vertical direction.
  • N detection components are respectively installed on the first pole, the second pole and the third pole.
  • the Chinese patent application with publication number CN108227774A discloses a device that expands the temperature control range of the photosynthesis measurement system by circulating alcohol.
  • the circulating pump is used to transport cooled or heated liquid alcohol, and is sent by a PVC pipe to the LI-6400XT photosynthesis measurement system.
  • the metal part that is tightly attached to the chamber which contains multiple water channels that can circulate alcohol, and changes the temperature of the metal part; gradually adjusts the temperature of the photosynthesis measurement chamber through the heat exchange process, and ultimately effectively expands the portable photosynthesis measurement system ( LI-6400XT) temperature control range.
  • This device allows the LI-6400XT portable photosynthesis measurement system to expand the range of leaf chamber temperature control from the original ambient temperature of ⁇ 7°C to 5°C to 40°C, so that the field measurement of plant/crop photosynthesis parameters is no longer affected by the external environment temperature. It provides measurement technology and methods for in-depth exploration of the response and adaptability mechanism of plant/crop physiological and ecological processes to temperature under field conditions. Since this invention has similar shortcomings to the photosynthetic meter, this invention can only be used for quantitative detection of some leaves and is not suitable for high-quality photosynthetic detection of single-sided leaves.
  • the Chinese patent application with publication number CN 209525597U discloses a photosynthesis meter that automatically adjusts the direction, including a base.
  • the photosynthesis meter is set on the base.
  • the photosynthesis meter is set on the base through a turntable.
  • the lower end of the turntable is equipped with a transmission. gear, a rotating motor is arranged next to the base, a speed change gear is arranged on the rotating shaft of the rotation motor, the transmission gear meshes with the speed change gear, a photosensitive sensor is arranged around the photosynthesis meter, and the direction of real-time sunlight is detected through the photosensitive sensor and the rotation is controlled
  • the motor drives the turntable to rotate. Photosynthesis is detected by a photosynthesis measuring instrument, and the turntable is driven by a rotating motor to rotate horizontally. Photosynthesis cannot be measured on single-sided leaves at different angles.
  • the Chinese patent application with publication number CN109900643A discloses a rapid measurement device for photosynthetic rate and transpiration rate of cotton boll leaf system, including a plant photosynthetic meter host, an IRGA sample chamber of an IRGA analyzer, and a cluster leaf chamber mounting plate.
  • the IRGA sample chamber of the IRGA analyzer is connected to the host of the plant photosynthesis meter through a cable.
  • the characteristic is that the IRGA sample chamber of the IRGA analyzer removes the cover plate and is connected to the cluster leaf chamber installation plate.
  • a self-made leaf chamber is installed on the chamber mounting plate, and a vent hole is provided on the connecting plate. Through the vent hole, the self-made leaf chamber, the cluster leaf chamber mounting plate and the IRGA sample chamber are connected.
  • this invention uses a fan to circulate the self-made blade chamber, it cannot make the air in the blade chamber uniform, and cannot measure blades at different angles.
  • the size of the blade chamber cannot be adjusted with the size of the blade, and is not suitable for high-precision measurement of a single blade, let alone Suitable for single-sided photosynthetic detection of plants.
  • the Chinese patent application with publication number CN113063902 A discloses a portable analysis system for real-time measurement of plant photosynthesis in the field, which consists of an external circulation leaf chamber, a carbon dioxide absorption pool, a circulation pump, a carbon dioxide concentration detector, a carbon dioxide storage tank and multiple It consists of an angle fixed frame.
  • the external circulation leaf chamber is fixed on the multi-angle fixed frame.
  • the carbon dioxide storage tank, carbon dioxide absorption pool and circulation pump are connected to the external circulation leaf chamber through organic hoses and valves.
  • the carbon dioxide concentration detector is placed in the external circulation leaf chamber. In the circulating leaf chamber.
  • This system can make the carbon dioxide concentration in the leaf chamber exactly the same, avoid the uneven carbon dioxide concentration caused by photosynthesis in the leaf chamber, provide the feasibility for long-term measurement of photosynthetic intensity experiments of the entire plant or a single leaf, and can also effectively slow down the It prevents the temperature rise in the closed environment of the leaf chamber and effectively maintains the stability of the ambient humidity. However, it is impossible to detect the intensity of photosynthesis on one side of the plant.
  • Tanaka Takayuki and Matsushima Shozo's "Application of Rice Yield Establishment Principles and Research in Crop Science” also mentioned the method of detecting the photosynthesis intensity of a single leaf, but it cannot be detected after photosynthesis is induced on a certain side of the leaf. It can be seen that neither the measurement method using the classic Licor-6800 photosynthesis meter or similar improved devices, nor the existing real-time online large-area detection photosynthesis method can accurately measure the photosynthesis intensity produced by a single leaf of a plant. For the purpose, no device has been reported to detect photosynthesis after inducing photosynthesis on one side of the leaf.
  • the problems that need to be solved are: First, it can better achieve accurate measurement in isolation from the outside air under natural light or artificial light sources, and can effectively maintain the stability of carbon dioxide concentration; Second, it can accurately induce one of the two sides of the plant leaves to perform photosynthesis, while the other side of the leaves does not perform photosynthesis. It is possible to measure the photosynthesis intensity on one or both sides for a long time without damaging the leaves during the entire detection process, thereby avoiding the defects of the existing technology.
  • the purpose of the present invention is to overcome the shortcomings of existing equipment and provide a method for inducing photosynthetic intensity of single-sided leaves with different growth angles, high accuracy, no damage to the leaves, portable, and real-time carbon 13 labeling. device.
  • the present invention is based on the traditional leaf chamber or an external leaf chamber.
  • the air in the external leaf chamber is circulated through a circulation pump, and a sealing ring is used to isolate it from the outside air.
  • the external leaf chamber can be placed on the plant leaves. It is completely separated when entering, and the external leaf chamber can be installed with artificial controllable light sources.
  • the external leaf chamber is fixed by a multi-angle fixing frame for leaves with different growth angles.
  • one of the principles of the present invention is that, on the basis of traditional leaf chamber detection of photosynthetic intensity, the air flow in the leaf chamber is circulated so that the measured oxygen and carbon dioxide concentrations in all parts of a single leaf surface are consistent, avoiding the direct influence of different carbon dioxide concentrations.
  • the photosynthesis speed causes the measured values to generally decrease (causing systematic errors).
  • the second principle of the invention is that the leaf chambers are separated by plant leaves to form two completely independent air circulation systems. Carbon 13-labeled carbon dioxide is added to one of the systems to achieve the purpose of tracking the distribution and transport of photosynthetic products on both sides of the plant leaves.
  • the third principle of the present invention is that based on the technology of traditional external circulating leaf chambers, the use of fully transparent materials such as organic glass can directly use the irradiation of natural light to solve the light source problem of photosynthesis, and combine with the sealing ring to isolate it from the outside air. Avoid interference from external conditions and achieve photosynthetic intensity measurement of a single leaf surface under natural lighting conditions.
  • the fourth principle of the present invention is that on the basis of the traditional external circulating leaf chamber, by adding an artificial adjustable light source, the light intensity can be controlled, and the photosynthesis measurement of a single leaf surface can be induced under artificial light source conditions, ensuring that the other leaf surface Basically no photosynthesis occurs.
  • the fifth principle of the present invention is that in order to ensure the measurement of leaves with different growth angles, a multi-angle fixing device is used to realize multi-directional fixed external leaf chambers, so that the growth status of the leaves is not changed when measuring photosynthetic intensity, which is more conducive to keeping the measured leaves consistent with The good growth status of the plant prevents the leaf veins from being blocked, damaged or damaged due to the measurement of photosynthetic intensity.
  • the object of the present invention is to provide a device for inducing single-sided leaf photosynthesis of plants.
  • the device includes: a leaf chamber, a circulation pump, a carbon dioxide supply device, a carbon dioxide absorption pool, a carbon dioxide detection device and a small number of valves and organic hoses.
  • the leaf chamber is composed of organic glass and a sealing ring.
  • a detachable artificial light source device is installed on the periphery of the leaf chamber.
  • the circulation pump is connected to the leaf chamber through an organic hose.
  • the carbon dioxide detection device consists of a carbon dioxide concentration detection probe and a carbon dioxide detector. It is composed and connected through optical fiber, and the carbon dioxide concentration detection probe is placed in the leaf chamber.
  • the leaf chamber is made of plant leaves that can be placed in organic glass and a sealing ring, and can be divided therefrom to form two independent spaces, which are two external circulation leaf chambers.
  • the leaf chamber can be illuminated by an artificial light source device.
  • the artificial light source is installed on the periphery of the leaf chamber.
  • the illumination intensity can be adjusted and removed when the photosynthetic intensity is detected under natural conditions.
  • the organic glass is made of a material with a light transmittance of more than 80%, a thickness of 1-10 mm, and a shape of cylindrical, rectangular, spherical, etc.
  • the multi-angle fixation bracket can be fixed at any angle.
  • the depth at which the carbon dioxide concentration detection probe is placed in the external circulation leaf chamber is 5 to 50 mm, with 15 mm being preferred.
  • the carbon dioxide absorption pool is composed of 0.1 to 0.5 mol/L sodium hydroxide aqueous solution.
  • the amount of carbon dioxide absorbed is converted by measuring the change in pH in the solution or directly measuring the amount of carbon dioxide produced by the neutralization reaction of sulfuric acid.
  • the temperature of the solution can also be controlled through cooling equipment, which directly affects the temperature changes in the external circulation leaf chamber.
  • the carbon dioxide supply device consists of a carbon dioxide storage tank with a pressure higher than 1 atmosphere or carbon dioxide produced by the reaction of sodium carbonate and dilute sulfuric acid.
  • the carbon dioxide emission rate is determined by an electronically controlled valve, and
  • the circulation pump correlates the air circulation speed and keeps the carbon dioxide concentration in the organic hose at 0.03%, and then makes appropriate adjustments with the carbon dioxide detector detection data.
  • the device can be used as a carbon marker or other marker for plants.
  • Figure 1 Schematic diagram of the device for inducing photosynthesis in single-sided leaves; 1: organic glass; 2: organic glass; 3: carbon dioxide concentration detection probe; 4: carbon dioxide concentration detection probe; 7: carbon dioxide detector; 8: carbon dioxide detector; 9: Circulation pump; 10: Circulation pump; 11: Organic hose; 12: Organic hose; 13: Three-way valve; 14: Three-way valve; 15: Carbon dioxide supplier; 16: Carbon dioxide supplier; 17: Carbon dioxide absorption Pool; 18: Carbon dioxide absorption pool; 19: Sealing ring; 20: Multi-angle fixing frame; 21: Metal support rod.
  • Figure 2 Schematic diagram of the device for inducing single-sided leaf photosynthesis; 1: organic glass; 2: organic glass; 3: carbon dioxide concentration detection probe; 4: carbon dioxide concentration detection probe; 5: light source; 6: light source; 7: carbon dioxide detection Instrument; 8: Carbon dioxide detector; 9: Circulation pump; 10: Circulation pump; 11: Organic hose; 12: Organic hose; 13: Three-way valve; 14: Three-way valve; 15: Carbon dioxide supplier; 16: Carbon dioxide supplier; 17: carbon dioxide absorption pool; 18: carbon dioxide absorption pool; 19: sealing ring; 20: multi-angle fixed frame; 21: metal support rod.
  • the stomata of the leaves on one side of the plant can be basically closed, so that the leaves on the other side of the plant can perform photosynthesis normally, ensuring that the other leaf side is basically in a state of no photosynthesis, effectively inducing photosynthesis on one side of the plant's leaves. purpose of action.
  • This device can separate both sides of the leaves for photosynthesis, and can effectively achieve the purpose of studying the different photosynthetic mechanisms on both sides of the plant leaves through carbon labeling or other markers.
  • the external circulation pump ensures that the carbon dioxide concentration on both sides of the plant leaves in the leaf chamber is exactly the same, avoiding uneven carbon dioxide concentration caused by inconsistent photosynthesis intensity on both sides.
  • FIG. 1 a schematic diagram of the device for inducing photosynthesis in a single-sided leaf.
  • the organic glass (1) and the sealing ring (19) are combined to form an external circulation leaf chamber A.
  • An artificial light source (5) is installed outside the external circulation leaf chamber A.
  • the external circulation leaf chamber A is connected to the external circulation leaf chamber A through an organic hose.
  • the circulation pump (9) is connected, the carbon dioxide absorption pool (17) is connected with the circulation pump (9) through an organic hose, the carbon dioxide concentration detection probe (3) is placed in the external circulation leaf chamber A, and the carbon dioxide concentration detection probe (3) passes through
  • the optical fiber is connected to the carbon dioxide detector (7).
  • the carbon dioxide supplier (15) is connected to the external circulation chamber A through the organic hose (11) through the three-way valve (13).
  • the three-way valve (13) controls the carbon dioxide flow rate or Turn off the carbon dioxide supply, and the carbon dioxide absorption pool (17) is connected to the external circulation leaf chamber A through the three-way valve (13) through the organic hose (11);
  • the organic glass (2) and the sealing ring (19) are combined to form an external circulation leaf chamber B.
  • An artificial light source (6) is installed outside the external circulation leaf chamber B.
  • the external circulation leaf chamber B communicates with the circulation pump ( 10) is connected.
  • the carbon dioxide absorption pool (18) is connected to the circulation pump (10) through an organic hose.
  • the carbon dioxide concentration detection probe (4) is placed in the external circulation leaf chamber B.
  • the carbon dioxide concentration detection probe (4) communicates with the carbon dioxide through an optical fiber.
  • the detector (8) is connected, and the carbon dioxide supplier (16) is connected to the external circulation chamber B through the organic hose (12) through the three-way valve (14).
  • the three-way valve (14) controls the carbon dioxide flow rate or closes the carbon dioxide supply.
  • the carbon dioxide absorption pool (18) is connected to the external circulation leaf chamber B through the three-way valve (14) and the organic hose (12); the external circulation leaf chambers A and B are fixed at multiple angles through metal struts (21). Put on the rack (20).
  • Example 1 Method for inducing real-time detection of photosynthesis in single-sided leaves, see Figure 1.
  • the external circulation leaf chamber A is connected to the circulation pump (9) through an organic hose
  • the carbon dioxide absorption pool (17) is connected to the circulation pump (9) through an organic hose
  • the carbon dioxide concentration detection probe (3) is placed outside Placed in the circulation leaf chamber A
  • the carbon dioxide concentration detection probe (3) is connected to the carbon dioxide detector (7) through an optical fiber
  • the carbon dioxide supplier (15) passes through the three-way valve (13) and the organic hose (11) to the external circulation
  • the leaf chamber A is connected, and the three-way valve (13) controls the flow rate of carbon dioxide or shuts off the supply of carbon dioxide.
  • the carbon dioxide absorption pool (17) is connected to the external circulation leaf chamber A through the organic hose (11) through the three-way valve (13);
  • the artificial light source (6) placed outside the circulating leaf chamber B is in a closed state.
  • Use the carbon dioxide detector (7) to detect data to control the carbon dioxide concentration in the leaf chamber to be maintained at 0.03%.
  • Example 2 A method for detecting photosynthesis of plant leaves under natural light conditions using any of the above devices, as shown in Figure 2.
  • the plexiglass (1), (2) sealing ring (19) and the plant leaves together form a sealed external circulation leaf chamber A
  • External circulation leaf chamber B, external circulation leaf chamber A are connected to the circulation pump (9) through an organic hose
  • the carbon dioxide absorption pool (17) is connected to the circulation pump (9) through an organic hose
  • the carbon dioxide concentration detection probe (3) Placed in the external circulation leaf chamber A
  • the carbon dioxide concentration detection probe (3) is connected to the carbon dioxide detector (7) through an optical fiber
  • the carbon dioxide supplier (15) is connected to the organic hose (11) through the three-way valve (13).
  • the external circulation leaf chamber A is connected.
  • the three-way valve (13) controls the carbon dioxide flow rate or turns off the carbon dioxide supply.
  • the carbon dioxide absorption pool (17) is connected to the external circulation leaf chamber A through the three-way valve (13) and the organic hose (11). ;
  • the external circulation leaf chamber B is connected to the circulation pump (10) through an organic hose, the carbon dioxide absorption pool (18) is connected to the circulation pump (10) through an organic hose, and the carbon dioxide concentration detection probe (4) is placed in the external circulation leaf In chamber B, the carbon dioxide concentration detection probe (4) is connected to the carbon dioxide detector (8) through an optical fiber, and the carbon dioxide supplier (16) passes through the three-way valve (14) and the organic hose (12) to the external circulation leaf chamber B.
  • the three-way valve (14) controls the carbon dioxide flow rate or turns off the carbon dioxide supply.
  • the carbon dioxide absorption pool (18) is connected to the external circulation leaf chamber B through the three-way valve (14) through the organic hose (12); with the help of the carbon dioxide detector ( 7) and (8) detect data to control the carbon dioxide concentration in the leaf chamber to remain at 0.03%.
  • Example 3 Method for real-time detection of photosynthesis on a certain leaf using carbon 13-labeled carbon dioxide
  • the plexiglass (1), (2) sealing ring (19) and the plant leaves together form a sealed external circulation leaf chamber A
  • External circulation leaf chamber B, external circulation leaf chamber A are connected to the circulation pump (9) through an organic hose
  • the carbon dioxide absorption pool (17) is connected to the circulation pump (9) through an organic hose
  • the carbon dioxide concentration detection probe (3) Placed in the external circulation leaf chamber A
  • the carbon dioxide concentration detection probe (3) is connected to the carbon dioxide detector (7) through an optical fiber
  • the carbon dioxide supplier (15) is connected to the organic hose (11) through the three-way valve (13).
  • the external circulation leaf chamber A is connected.
  • the three-way valve (13) controls the carbon dioxide flow rate or turns off the carbon dioxide supply.
  • the carbon dioxide absorption pool (17) is connected to the external circulation leaf chamber A through the three-way valve (13) and the organic hose (11). ;
  • the external circulation leaf chamber B is connected to the circulation pump (10) through an organic hose, the carbon dioxide absorption pool (18) is connected to the circulation pump (10) through an organic hose, and the carbon dioxide concentration detection probe (4) is placed in the external circulation leaf In chamber B, the carbon dioxide concentration detection probe (4) is connected to the carbon dioxide detector (8) through an optical fiber, and the carbon dioxide supplier (16) passes through the three-way valve (14) and the organic hose (12) to the external circulation leaf chamber B.
  • the three-way valve (14) controls the carbon dioxide flow rate or turns off the carbon dioxide supply, and carbon dioxide in the carbon dioxide supplier (16) is carbon 13 labeled.
  • the carbon dioxide absorption pool (18) passes through the organic hose (14) through the three-way valve (14).
  • 12) Connected to the external circulation leaf chamber B; use carbon dioxide detectors (7) and (8) to detect data to control the carbon dioxide concentration in the leaf chamber to remain at 0.03%.

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Abstract

一种诱导单面叶片光合作用装置，包括：叶室、循环泵、二氧化碳供给装置、二氧化碳吸收池、二氧化碳检测装置及少量阀门和有机软管组成，叶室由有机玻璃（1）与密封圈（19）构成，叶室外围安装可拆卸的人工光源（5），循环泵（9）通过有机软管与叶室相连接，二氧化碳检测装置由二氧化碳浓度检测探头（3）和二氧化碳检测仪（7）组成，并且通过光纤进行连接，二氧化碳浓度检测探头（3）置入叶室中。通过人工光源（5）控制使植株某面叶片的气孔基本关闭，有效达到诱导植物单面叶片光合作用的目的；通过循环泵（9）保证叶室中植株叶片两面的二氧化碳浓度完全相同，避免了因两面光合作用强度不一致以造成二氧化碳浓度不均。

Description

一种诱导单面叶片光合作用装置 技术领域
属于植物叶片光合检测技术领域，涉及一种诱导单面叶片光合作用装置，具体涉及一种在整株生长状态下诱导某一叶片单面光合作用强度的检测，并对某一叶片单面光合产物进行 13 CO 2 标记的叶室。
 背景技术
目前，市场上有Licor-6800、LCi-SD、CIRAS-3等便携式光合仪，为植物生态学研究提供了光合作用测定技术手段，该类光合仪比较适用于室内叶片光合实验。但在野外对某一植株在正常生长状态下检测单面叶片光合作用强度实验时，由于现有野外检测设备未考虑到叶片正反面受到光照强度不一，以及叶片存在正反面气孔数量完全不同等情况，造成检测光合作用强度数据随意性非常大，因此在植物品种筛选时，一是叶片光合作用强度数据支持力度不大，二是无法区分和评价正反面光合速率，不能准确高效对种质资源进行光合速率评估。
公开号为CN203981676U的中国专利申请公开了一种整株组培苗光合速率快速测定装置及方法，包括植物光合测定仪的IRGA样品室，土壤叶室安装板，IRGA样品室与土壤叶室安装板组成复合体，另设有一个透明的自制叶室，在自制叶室上设有固定孔、气孔及回流管接口；自制叶室通过固定孔与土壤叶室安装板连接，自制叶室与复合体之间为刚性密封连接；IRGA样品室通过数据线与光合仪主机连接。该发明虽对现有的采用植物光合测定仪进行改进，采用自制叶室来替代了标准叶室，光合仪能直观地实时显示植株在不受任何机械压迫损伤情况下的光合速率，并可以任意测定单株或者整瓶(群体)组培苗的光合速率；还解决了标准叶室没法测定面积小于6cm 2 的单张叶片的难题，但还是无法对某一植株单面叶片的光合作用进行测定。
公开号为CN205080115U的中国专利申请公开了一种植物合速率测定装置，包括LED灯，提供植物光源，可以调节光强变化；气泵，输送CO 2 气体；叶室，固定测量叶片；流量计，检测CO 2 气体流量；CO 2 分析器，检测CO 2 浓度；温湿度传感器，检测CO 2 气体温湿度变化；数据采集器，收集与记录采集到的数据；计算机，分析采集到的数据。但无法控制CO 2 浓度和完成单面叶片的光合检测。
公开号CN207571108U的中国专利申请公开了一种测定作物群体不同高度处光合速率、呼吸速率和温室气体排放的装置，包括测试箱、第一支杆、第二支杆、第三支杆、3N个检测组件和信号采集器，测试箱包括顶盖、箱体和底座，箱体顶部上设有第一凹槽，箱体内壁上安装有风扇，箱体内壁上对称安装有一对支撑架，第一支杆、第二支杆、第三支杆的两端分别通过可拆卸地方式固定在两支撑架上，第一支杆、第二支杆、第三支杆在竖直方向上等间距分布，第一支杆、第二支杆和第三支杆上分别安装N个检测组件，底座顶部设有的第二凹槽，各电元器件分别与信号采集器电连接。该方法依托于该装置，能测定作物群体不同高度处的光强、温湿度、光合速率、呼吸速率以及温室气体的排放，但该专利也未能考虑到某一叶片单面光合作用强度的测定。
公开号为CN108227774A的中国专利申请公开了一种通过循环酒精扩展光合作用测定系统控温范围的装置，利用循环泵输送冷却或加热的液体酒精，并由PVC管送至与LI-6400XT光合作用测量室紧紧贴合的金属部件(内部含有多条可流通酒精的水道)，并改变该金属部件的温度；通过热交换过程逐渐调整光合作用测量室的温度，最终有效扩展便携式光合作用测定系统(LI-6400XT)的控温范围。该装置使得LI-6400XT便携式光合测定系统调控叶室温度的范围由原有的环境温度±7℃扩展为5℃～40℃，从而使得野外测定植物/作物光合作用参数不再受外界环境温度的限制，为野外条件下深入探讨植物/作物生理生态过程对温度的响应和适应性机理提供了测量技术与方法。由于该发明存在和光合仪类似的缺点，因此该发明只能用于部分叶片的定量检测，并不适合单面叶片的高质量光合检测。
公开号为CN 209525597U的中国专利申请公开了一种自动调整方向的光合作用测定仪，包括底座，底座上设置有光合作用测定仪，光合作用测定仪通过转盘设置在底座上，转盘下端设置有传动齿轮，底座旁设置有转动电机，转动电机的转轴上设置有变速齿轮，传动齿轮与变速齿轮啮合，光合作用测定仪四周设置有光敏传感器，通过光敏传感器对实时阳光的方向进行检测，并且控制转动电机带动转盘进行转动。通过光合作用测定仪进行光合作用检测，并且通过转动电机带动转盘水平转动，无法对不同角度的单面叶片进行光合测定。
公开号为CN109900643A的中国专利申请公开了一种棉花铃叶系统光合速率和蒸腾速率的快速测定装置，包括植物光合测定仪主机、IRGA分析器的IRGA样品室、簇状叶室安装板，所述IRGA分析器的IRGA样品室通过电缆线与植物光合测定仪主机相连接，其特征在于所述IRGA分析器的IRGA样品室去除盖板，与簇状叶室安装板连接，在所述簇状叶室安装板上安装有自制叶室，所述连接板上设有通气孔，通过该通气孔，使得所述自制叶室、簇状叶室安装板与IRGA样品室相连通。该发明虽通过风扇使自制叶室循环，但无法使叶室中空气均匀，对不同角度的叶片无法进行测定，叶室大小不能随叶片大小调整，并不适合单一叶片的高精度测定，更不适合植株单面光合检测。
公开号为CN113063902 A的中国专利申请公开了一种便携式用于野外实时测定植物光合作用的分析系统，由外置循环叶室、二氧化碳吸收池、循环泵、二氧化碳浓度检测器、二氧化碳储存罐和多角度固定架组成，外置循环叶室固定在多角度固定架上，二氧化碳储存罐、二氧化碳吸收池、循环泵通过有机软管和阀门与外置循环叶室连通，二氧化碳浓度检测器置入在外置循环叶室中。该系统能使叶室内的二氧化碳浓度完全相同，避免了在叶室中因光合作用造成二氧化碳浓度不均，为长时间测定整个植物或单一叶片的光合强度实验提供可行性，同时还能有效地减缓了叶室封闭环境温度上升，有效地维持了环境湿度的稳定。但无法完成对植株单面光合作用强度的检测。
田中孝幸·松島省三在“水稻产量成立原理应用及在作物学的研究”也提及了单一叶片的光合作用强度检测方法，但无法诱导对叶片某一面光合作用后进行检测。由此可见，无论是使用经典Licor-6800合光测定仪或类似改进装置的测定方法，还是现有的实时在线大面积检测光合方法，都无法满足精确测定植株单面叶片所产生的光合作用强度的目的，更未报道过诱导叶片一面光合作用后进行检测的装置。
 发明内容
因此，实现植株单面叶片进行光合作用检测时，需要解决的问题在于：一是在自然光或人工光源照射条件下能更好地实现与外界空气隔绝进行精确测定，能有效维持二氧化碳浓度的稳定；二是能精确诱导植株叶片两面中某一面叶片进行光合作用，且另一面叶片不进行光合作用。实现整个检测过程中在不损伤叶片条件下长时间测定单面或两面光合作用强度，从而避免现有技术存在的缺陷。
因此，本发明的目的是要克服现有设备的不足之处，提供一种不同生长角度叶片、高准确性、不损伤叶片、便携式、可实现碳13标记的实时诱导单面叶片光合作用强度的装置。
为了实现上述技术目的，本发明在传统叶室或外置叶室的基础上，通过循环泵使外置叶室内空气循环，采用密封圈与外界空气隔绝，且外置叶室能在植株叶片置入时完全分开，外置叶室可安装人工可控制光源，通过多角度固定架固定外置叶室用于不同生长角度的叶片。
因此，本发明原理之一在于，在传统叶室检测光合强度的基础上，将循环叶室内的空气流动，使得所测单一叶面各个部位的氧气和二氧化碳浓度一致，避免因二氧化碳浓度不同直接影响其光合作用速度而使测定数值普遍下降(造成系统误差)。
本发明原理之二在于，通过植株叶片将叶室分隔形成两个完全独立的空气循环体系，其中一个体系中添加碳13标记的二氧化碳，可以实现追踪植株叶片两面的光合产物分配转运的目的。
本发明原理之三在于，在传统外置循环叶室的技术上，使用全透光材料如有机玻璃等材料，可直接利用自然光的照射解决光合作用的光源问题，结合密封圈与外界空气隔绝，避免外界条件下干扰，实现单一叶面在自然光照条件下的光合强度测定。
本发明原理之四在于，在传统外置循环叶室的基础上，通过外加人工可调节光源，达到可控制光照强度，实现诱导单一叶面在人工光源条件下光合作用测定，保证另一叶面基本不进行光合作用。
本发明原理之五在于，为了保证测定不同生长角度的叶片，使多角度固定装置实现多方向固定外置叶室，在测定光合强度时不改变叶片的生长状态，更有利于保持所测叶片与植株的良好生长状态，避免了叶片因测定光合强度造成叶脉堵塞、损伤或损坏掉。
因此，本发明的目的是提供一种诱导植物单面叶片光合作用装置，该装置包括：叶室、循环泵、二氧化碳供给装置、二氧化碳吸收池、二氧化碳检测装置及少量阀门和有机软管组成，所述叶室由有机玻璃与密封圈构成，叶室外围安装可拆卸的人工光源装置，所述循环泵通过有机软管与叶室相连接，所述二氧化碳检测装置由二氧化碳浓度检测探头和二氧化碳检测仪组成，并且通过光纤进行连接，二氧化碳浓度检测探头置入叶室中。
在上述任一的实施方案中，所述叶室由植物叶片可置入有机玻璃与密封圈中，并能与之分割形成两个独立的空间，为两个外置循环叶室。
在上述任一的实施方案中，所述叶室可由人工光源装置提供光照，人工光源安装在叶室外围，光照强度可以进行调节，在自然条件下检测光合强度时去除。
在上述任一的实施方案中，所述有机玻璃的材质为透光率达到80％以上，其厚度为1-10mm，其形状为圆柱形或长方形或球形等。
在上述任一的实施方案中，所述多角度固定架为任意角度的固定。
在上述任一的实施方案中，所述二氧化碳浓度检测探头放入外置循环叶室的深度为5～50mm，其中优选是15mm。
在上述任一的实施方案中，所述二氧化碳吸收池，由0.1～0.5mol/L氢氧化钠水溶液组成，通过测定溶液中pH的变化换算出二氧化碳吸收量或直接测定硫酸中和反应产二氧化碳的量，还可通过降温设备进行控制其溶液的温度，直接影响外置循环叶室内的温度变化。
在上述任一的实施方案中，所述二氧化碳供给装置，由高于1个大气压的二氧化碳储存罐或由碳酸钠与稀硫酸反应生产二氧化碳，都是通过电子控制的阀门决定二氧化碳的排放速度，与循环泵使空气循环速度相关，保持有机软管中二氧化碳浓度在0.03％即可，再与二氧化碳检测仪检测数据进行适当调整。
在上述任一的实施方案中，该装置可用作为植株的碳标记或其它标记等方面的应用。
 附图说明
图1、诱导单面叶片光合作用装置的示意图；其中1：有机玻璃；2：有机玻璃；3：二氧化碳浓度检测探头；4：二氧化碳浓度检测探头；7：二氧化碳检测仪；8：二氧化碳检测仪；9：循环泵；10：循环泵；11：有机软管；12：有机软管；13：三通阀门；14：三通阀门；15：二氧化碳供给器；16：二氧化碳供给器；17：二氧化碳吸收池；18：二氧化碳吸收池；19：密封圈；20：多角度固定架；21：金属支杆。
图2、诱导单面叶片光合作用装置的示意图；其中1：有机玻璃；2：有机玻璃；3：二氧化碳浓度检测探头；4：二氧化碳浓度检测探头；5：光源；6：光源；7：二氧化碳检测仪；8：二氧化碳检测仪；9：循环泵；10：循环泵；11：有机软管；12：有机软管；13：三通阀门；14：三通阀门；15：二氧化碳供给器；16：二氧化碳供给器；17：二氧化碳吸收池；18：二氧化碳吸收池；19：密封圈；20：多角度固定架；21：金属支杆。
技术效果
1、通过人工光源控制，可以使植株某面叶片的气孔基本关闭，从而实现植株另一面叶片能正常进行光合作用，保证另一叶面基本不进行光合作用状态，有效达到诱导植物单面叶片光合作用的目的。
2、该装置能将叶片两面分开进行光合作用，可通过碳标记或其它标记有效实现植株叶片两面不同光合机理研究的目的。
3、通过外循环泵保证叶室中植株叶片两面的二氧化碳浓度完全相同，避免了因两面光合作用强度不一致以造成二氧化碳浓度不均。
4、通过先吸收空气中二氧化碳、后期重新补充二氧化碳的方式，为长时间测定整个植物或单一叶片的光合强度实验提供可行性，同时还能有效地减缓了叶室封闭环境温度上升，有效地维持了环境湿度的稳定。
5、通过多角度固定架在测定光合时，不会影响测定植物不同生长角度叶片的光合强度，也更加方便实时比较不同生长角度叶片的光合强度，而且大大减轻了操作人员的工作强度，为植物光合基础研究和生物工程研究提供了一种非常高效的工具。
 实施方式
下面结合附图和实施例对本发明进一步说明。但以下所述，仅是本发明的较佳实施例而已，并非对本发明作任何形式上的限制，故凡是未脱离本发明技术方案内容，依据本发明的技术实质对以上实施例所作的任何简单修改、等同变化与修饰，均仍属于本发明技术方案的范围内。
如图1所示，诱导单面叶片光合作用装置的示意图。其中，将有机玻璃(1)与密封圈(19)进行合并构成外置循环叶室A，在外置循环叶室A外安装有人工光源(5)，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；
有机玻璃(2)与密封圈(19)进行合并构成外置循环叶室B，在外置循环叶室B外安装有人工光源(6)，外置循环叶室B通过有机软管与循环泵(10)连通，二氧化碳吸收池(18)通过有机软管与循环泵(10)连通，二氧化碳浓度检测探头(4)置入在外置循环叶室B内，二氧化碳浓度检测探头(4)通过光纤与二氧化碳检测仪(8)相连通，二氧化碳供给器(16)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通，三通阀门(14)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(18)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通；外置循环叶室A、B通过金属支杆(21)固定在多角度固定架(20)上。
实施例1：诱导单面叶片实时检测光合作用的方法，见图1。
首先将植物叶片取出，采用人工光源(5)进行光照，通过观察叶片上气孔关闭状态，调节适合的光照强度，再将有机玻璃与密封圈打开，选择植物叶片放入有机玻璃与密封圈构成的间隙中，有机玻璃、密封圈和植物叶片一并形成完全独立的外置循环叶室A、外置循环叶室B，打开人工光源(5)，调节适合的光照强度使另一叶面的气孔处于关闭状态，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；而外置循环叶室B外的人工光源(6)处于关闭状态。借助二氧化碳检测仪(7)检测数据以控制叶室中二氧化碳浓度保持在0.03％。通过测定二氧化碳吸收池(17)的pH值变化(吸收二氧化碳的量)与二氧化碳供给器(16)(供给二氧化碳的量)的输入量，计算出单面光合过程中二氧化碳的消耗量。
实施例2：利用上述任一装置来进行植物叶片在自然光条件用下检测光合作用的方法，见图2。
首先将密封圈打开，选择植物叶片放入有机玻璃与密封圈构成的间隙中，有机玻璃(1)、(2)密封圈(19)和植物叶片一并形成密封的外置循环叶室A、外置循环叶室B，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；外置循环叶室B通过有机软管与循环泵(10)连通，二氧化碳吸收池(18)通过有机软管与循环泵(10)连通，二氧化碳浓度检测探头(4)置入在外置循环叶室B内，二氧化碳浓度检测探头(4)通过光纤与二氧化碳检测仪(8)相连通，二氧化碳供给器(16)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通，三通阀门(14)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(18)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通；借助二氧化碳检测仪(7)、(8)检测数据以控制叶室中二氧化碳浓度保持在0.03％。通过测定二氧化碳吸收池(17)、(18)的pH值变化(吸收二氧化碳的量)与二氧化碳供给器(15)、(16)(供给二氧化碳的量)的输入量，计算出整个光合过程中二氧化碳的消耗量。
实施例3：利用碳13标记的二氧化碳进行某一面叶片实时检测光合作用的方法
首先将密封圈打开，选择植物叶片放入有机玻璃与密封圈构成的间隙中，有机玻璃(1)、(2)密封圈(19)和植物叶片一并形成密封的外置循环叶室A、外置循环叶室B，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；外置循环叶室B通过有机软管与循环泵(10)连通，二氧化碳吸收池(18)通过有机软管与循环泵(10)连通，二氧化碳浓度检测探头(4)置入在外置循环叶室B内，二氧化碳浓度检测探头(4)通过光纤与二氧化碳检测仪(8)相连通，二氧化碳供给器(16)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通，三通阀门(14)控制二氧化碳流速或关闭二氧化碳供给，并且将二氧化碳供给器(16)中的二氧化碳进行碳13标记，二氧化碳吸收池(18)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通；借助二氧化碳检测仪(7)、(8)检测数据以控制叶室中二氧化碳浓度保持在0.03％。通过测定二氧化碳吸收池(17)、(18)的pH值变化(吸收二氧化碳的量)与二氧化碳供给器(15)、(16)(供给二氧化碳的量)的输入量，计算出整个光合过程中二氧化碳的消耗量。另外将植株取出，可以通过碳13标记观察碳的运行轨迹。
以上所述，仅是本发明的较佳实施例而已，并非对本发明作任何形式上的限制，故凡是未脱离本发明技术方案内容，依据本发明的技术实质对以上实施例所作的任何简单修改、等同变化与修饰，均仍属于本发明技术方案的范围内。

Claims (10)

  1. 一种诱导植物单面叶片光合作用装置，该装置包括：叶室、循环泵、二氧化碳供给装置、二氧化碳吸收池、二氧化碳检测装置及少量阀门和有机软管组成，所述有机玻璃(1)与密封圈(19)进行合并构成外置循环叶室A，在外置循环叶室A外安装有人工光源(5)，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；
    有机玻璃(2)与密封圈(19)进行合并构成外置循环叶室B，在外置循环叶室B外安装有人工光源(6)，外置循环叶室B通过有机软管与循环泵(10)连通，二氧化碳吸收池(18)通过有机软管与循环泵(10)连通，二氧化碳浓度检测探头(4)置入在外置循环叶室B内，二氧化碳浓度检测探头(4)通过光纤与二氧化碳检测仪(8)相连通，二氧化碳供给器(16)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通，三通阀门(14)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(18)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通；外置循环叶室A、B通过金属支杆(21)固定在多角度固定架(20)上。
  2. 一种诱导植物单面叶片光合作用装置，该装置包括：叶室、循环泵、二氧化碳供给装置、二氧化碳吸收池、二氧化碳检测装置及少量阀门和有机软管组成，所述将有机玻璃(1)与密封圈(19)进行合并构成外置循环叶室A，外置循环叶室A通过有机软管与循环泵(9)连通，二氧化碳吸收池(17)通过有机软管与循环泵(9)连通，二氧化碳浓度检测探头(3)置入在外置循环叶室A内，二氧化碳浓度检测探头(3)通过光纤与二氧化碳检测仪(7)相连通，二氧化碳供给器(15)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通，三通阀门(13)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(17)经三通阀门(13)通过有机软管(11)与外置循环叶室A连通；
    有机玻璃(2)与密封圈(19)进行合并构成外置循环叶室B，外置循环叶室B通过有机软管与循环泵(10)连通，二氧化碳吸收池(18)通过有机软管与循环泵(10)连通，二氧化碳浓度检测探头(4)置入在外置循环叶室B内，二氧化碳浓度检测探头(4)通过光纤与二氧化碳检测仪(8)相连通，二氧化碳供给器(16)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通，三通阀门(14)控制二氧化碳流速或关闭二氧化碳供给，二氧化碳吸收池(18)经三通阀门(14)通过有机软管(12)与外置循环叶室B连通；外置循环叶室A、B通过金属支杆(21)固定在多角度固定架(20)上。
  3. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述有机玻璃(1)和(2)的材质为透光率达到80％以上，其厚度为1-10mm，其形状为圆柱形或长方形或球形等。
  4. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述多角度固定架(20)为任意角度的固定。
  5. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述二氧化碳浓度检测探头(3)和(4)分别放入外置循环叶室A、B的深度为5～50mm，其中优选是15mm。
  6. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述二氧化碳吸收池(17)、(18)，由0.1～0.5mol/L氢氧化钠水溶液组成，通过测定溶液中pH的变化换算出二氧化碳吸收量或直接测定硫酸中和反应产二氧化碳的量，还可通过降温设备进行控制其溶液的温度，直接影响外置循环叶室A、B内的温度变化。
  7. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述二氧化碳供给器(15)、(16)，由高于1个大气压的二氧化碳储存罐或由碳酸钠与稀硫酸反应生产二氧化碳，都是通过电子控制阀门决定二氧化碳的排放速度，与循环泵(9)、(10)使空气循环速度相关，保持有机软管中二氧化碳浓度在0.03％即可，再与二氧化碳检测仪(7)、(8)检测数据进行调整。
  8. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，所述叶室由植物叶片可置入有机玻璃与密封圈中，并能与之分割形成两个独立的空间，为两个外置循环叶室。
  9. 根据权利要求1的一种诱导植物单面叶片光合作用装置，所述叶室由人工光源装置提供光照，人工光源安装在叶室外围，光照强度可以进行调节，在自然条件下检测光合强度时去除。
  10. 根据权利要求1或2的一种诱导植物单面叶片光合作用装置，作为植株的碳标记或其它标记等方面的应用。
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CN113063902A (zh) * 2021-04-25 2021-07-02 湖南杂交水稻研究中心 一种便携式用于野外实时测定植物光合作用的分析系统
CN113063903A (zh) * 2021-04-25 2021-07-02 湖南杂交水稻研究中心 一种可调节用于植物光合作用同位素标记装置
CN114814104A (zh) * 2022-04-28 2022-07-29 湖南杂交水稻研究中心 一种诱导单面叶片光合作用装置

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