WO2023204285A1 - Agent de régulation de croissance de plante contenant des cellules viables ou une culture de souche du genre bacillus en tant que principe actif, et son procédé d'utilisation - Google Patents

Agent de régulation de croissance de plante contenant des cellules viables ou une culture de souche du genre bacillus en tant que principe actif, et son procédé d'utilisation Download PDF

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WO2023204285A1
WO2023204285A1 PCT/JP2023/015839 JP2023015839W WO2023204285A1 WO 2023204285 A1 WO2023204285 A1 WO 2023204285A1 JP 2023015839 W JP2023015839 W JP 2023015839W WO 2023204285 A1 WO2023204285 A1 WO 2023204285A1
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plant
growth
bacillus
strain
plant growth
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PCT/JP2023/015839
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Japanese (ja)
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勝美 古瀬
菜穂 市川
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クミアイ化学工業株式会社
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Definitions

  • the present invention relates to a plant growth regulator containing a live or cultured Bacillus strain as an active ingredient, and its use.
  • Patent Document 1 Some strains of Bacillus bacteria used for disease control have been found to have a germination rate promoting effect on plant seeds (Patent Document 1); It is not known to directly increase yield.
  • microorganisms are suitable as the active ingredient for a new plant growth regulator.
  • As a result of intensive screening we found that microorganisms of the genus Bacillus ( We have succeeded in developing a new plant growth regulator containing live bacteria or a culture of this strain as an active ingredient.
  • Bacillus sp. which is the active ingredient of the plant growth regulator according to the present invention.
  • D747 Bacillus sp., which is the active ingredient of the plant growth regulator according to the present invention.
  • the present inventors discovered, completely unexpectedly, that this strain confers tolerance to environmental stress on crops, which is a completely new and extremely effective action and property.
  • the present invention was completed based on the above-mentioned new knowledge and as a result of further studies, and is a novel, useful, and highly safe agent that imparts tolerance to environmental stress in crops of an unprecedented type. It also provides.
  • the present invention is naturally derived, safe to use, and can contribute to improving agricultural production by regulating crop growth and imparting tolerance to environmental stress, increasing yield, and simultaneously controlling plant diseases. This was done for the purpose of providing microbial materials that can be used.
  • the present inventor investigated various aspects and found that live bacteria or cultures of Bacillus bacteria used for disease control regulate the growth of crops, and in addition, provide resistance to environmental stress.
  • the present inventors have discovered that the present invention has the effect of increasing the yield by doing so, and have found a way to utilize this effect, leading to the completion of the present invention.
  • embodiments of the present invention are as follows. (1) It is characterized by containing live bacteria or a culture of Bacillus subtilis or Bacillus amyloliquefaciens as an active ingredient, and having a plant growth-promoting or growth-inhibiting effect. Plant growth regulator. (2) Bacillus sp. strain D747, Bacillus subtilis strain HAI-0404, Bacillus subtilis Y1336 strain, Bacillus amyloliquefaciens strain s amyloliquefaciens) F727 strain 1. A plant growth regulator characterized in that it contains one or more living bacteria or cultures as an active ingredient, and has a plant growth-promoting or growth-inhibiting effect.
  • a plant growth regulator characterized by containing live bacteria or a culture of Bacillus sp. D747 strain as an active ingredient, and having a plant growth promoting effect or a growth suppressing effect.
  • the agent according to any one of (1) to (3) which is characterized by imparting environmental stress tolerance to plants in addition to the plant growth-promoting or growth-inhibiting effect.
  • the agent according to any one of (1) to (4) which has a plant disease controlling effect in addition to a plant growth promoting effect or a growth suppressing effect.
  • the agent according to any one of (1) to (5) characterized in that it regulates the growth of plants of the Solanaceae family.
  • the agent according to any one of (1) to (5) characterized in that it regulates the growth of leguminous plants.
  • the agent according to any one of (1) to (5) characterized in that it regulates the growth of Brassicaceae plants.
  • It is characterized by comprising a step of bringing one or more plant growth regulators according to any one of (1) to (9) into contact with a plant body and/or soil (especially rhizosphere soil).
  • the growth of crops can be regulated and resistance to environmental stress can be imparted by simply using a plant growth regulator containing living bacteria or cultures of single or multiple bacterial strains, which are natural products, as an active ingredient.
  • a plant growth regulator containing living bacteria or cultures of single or multiple bacterial strains, which are natural products, as an active ingredient.
  • Environmental stress in the present invention includes high temperature stress, low temperature stress, water stress, dry stress, salt stress, ultraviolet stress, wind stress, etc., but is not limited to the above. Also, mention may be made especially of high temperature stress and water stress.
  • An example of the active ingredient in the present invention is Bacillus sp. D747 strain.
  • This D747 strain was isolated from the air in Kikugawa City, Shizuoka Prefecture, and is a strain that was isolated from the air in Kikugawa City, Shizuoka Prefecture. : Postal code 292-0818 (2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan) on November 28, 2000, Bacillus sp. It has been internationally deposited as D747 (FERM BP-8234). Note that this strain is also commercially available, as described later, and can be easily obtained by anyone.
  • Morphological properties Morphology: Bacillus Size: Width 1.0-1.2 ⁇ m, length 3-5 ⁇ m Motility: + Epiphytic state of flagella: Perifelgate Endospore: + Spore position: Center Spore expansion: -
  • Bacillus sp. D747 strain is commercially available under the brand name Ecoshot (registered trademark).
  • Bacillus subtilis strain HAI-0404 Bacillus subtilis Y1336 strain, Bacillus amyloliquefaciens strain F727, etc.
  • Bacillus subtilis strain HAI-0404 is the trade name of Agrocare (registered trademark) hydrating powder
  • Bacillus subtilis strain Y1336 is the trade name of Batistar (registered trademark) hydrating powder
  • Bacillus amyloliquefaciens strain F727. is commercially available under the trade name STARGUS (registered trademark).
  • there are notations such as Bacillus, Bacillus, Bacillus, etc. in addition to Bacillus, but these are all Japanese notations of the scientific name "Bacillus" and are the same.
  • Examples of the present invention include those containing live bacteria or a culture of Bacillus sp. D747 as an active ingredient.
  • the D747 strain can be used alone, but live bacteria or cultures of mutants of the D747 strain can also be used alone or together with live bacteria or cultures of the D747 strain. I can do it.
  • the mutants have the bacteriological characteristics of the D747 strain described above and have plant growth regulating effects, and include natural mutant strains, mutant strains produced using ultraviolet rays or chemical mutagens, cell fusion strains, and gene mutants. Recombinant strains are also available.
  • the D747 strain contained in the plant growth regulator of the present invention includes mutants of the D747 strain.
  • the culture used in the present invention is obtained from a culture of bacteria belonging to the genus Bacillus.
  • the culture refers to a mixture of microbial cells and a medium.
  • Bacteria belonging to the genus Bacillus can be cultured according to conventional culture methods for bacteria belonging to the genus Bacillus, such as reciprocating shaking culture, jar fermenter culture, liquid culture in a culture tank, or solid culture. Examples include general media such as meat extract media, as well as media containing glucose, peptone, and yeast extract.
  • a solid medium such as a slant medium containing agar or a plate medium may also be used. By culturing, bacteria belonging to the genus Bacillus can be grown to obtain a desired culture.
  • Any carbon source that can be assimilated by the above bacterial strain can be used as a carbon source for the culture medium.
  • various synthetic or natural carbon sources that can be used by strains belonging to the genus Bacillus can be mentioned, such as glucose, arabinose, mannose, starch hydrolyzate, and molasses.
  • various synthetic or natural products that can be used by the strain can be used, including organic nitrogen-containing substances such as peptone, meat extract, yeast extract, and soybean meal.
  • trace nutrients such as inorganic salts such as table salt and phosphates, salts of metals such as calcium, magnesium, and iron, vitamins, amino acids, and nucleic acid-related substances may be added as necessary. I can do it. Furthermore, various additives such as antifoaming agents can be added as necessary.
  • Cultivation can be carried out under aerobic conditions such as shaking culture and aeration culture.
  • the appropriate culture temperature is 20 to 30°C, preferably 25 to 30°C
  • the pH is 5 to 8, preferably 6 to 7, and the culture period is 1 to 4 days, preferably 2 to 3 days.
  • the Bacillus strain of the active ingredient of the present invention cultivated as described above can be used as an active ingredient of a plant growth regulator in the form of a culture containing viable bacteria without separating the bacteria.
  • viable cells are separated from the above culture by a conventional method such as membrane separation or centrifugation, and if necessary, the cultured isolated cells themselves or their processed material (cultured isolated cells and other It is also possible to use mixtures of ingredients (such as mixtures of ingredients) as active ingredients.
  • dosage forms can also be used in the form of formulations in various dosage forms with various additives according to conventional methods for agricultural chemical formulations.
  • dosage forms include granules, emulsions, wettable powders, flowable preparations, and the like.
  • the concentration of viable bacteria contained in the plant growth regulator of the present invention is not particularly limited as long as it exhibits the desired effect; however, if the concentration of bacteria is too low, sufficient results may not be obtained; Even if the amount is increased, bacteria may be wasted, so for example, when preparing and using it as a liquid, it can be adjusted as appropriate within the range of 1 x 10 5 to 1 x 10 11 cfu/ml, preferably 1 A range of ⁇ 10 6 to 1 ⁇ 10 10 cfu/ml is preferred.
  • "cfu” means colony forming unit.
  • it may be designed appropriately according to the above-mentioned viable cell concentration.
  • Examples of plants whose growth regulation, resistance to environmental stress, and disease control are expected to be achieved by the plant growth regulator of the present invention include cereals (e.g., rice, wheat, barley, corn, and buckwheat), and tubers (e.g., potatoes, sweet potatoes). , taro, yam), legumes (e.g. soybeans, green beans, adzuki beans, peas), vegetables (e.g. cucurbits, tomatoes, eggplants, green peppers, cabbage, Chinese cabbage, radish, lettuce, carrots, green onions, onions, strawberries, spinach, sugar beets, beets, chard, celery), fruit trees (e.g.
  • cereals e.g., rice, wheat, barley, corn, and buckwheat
  • tubers e.g., potatoes, sweet potatoes.
  • legumes e.g. soybeans, green beans, adzuki beans, peas
  • vegetables e.g. cucur
  • the plant pathogenic bacteria that can be controlled by the plant growth regulator and/or plant disease control agent and/or environmental stress imparting agent of the present invention include bacteria of the genus Pseudoperonospora, such as cucumber Pseudoperonospora cubensis, Venturia sp., such as Venturia inaequalis, Erysiphe sp., such as wheat powdery mildew (Erysiphe gr. amines), Pyricularia spp., e.g.
  • Botrytis genus bacteria such as cucumber botrytis cinerea, Rhizoctonia genus bacteria, such as rice sheath blight fungus (Rhizoctonia) olani), Cladosporium spp.
  • Botrytis genus bacteria such as cucumber botrytis cinerea
  • Rhizoctonia genus bacteria such as rice sheath blight fungus (Rhizoctonia) olani)
  • Cladosporium spp For example, tomato leaf mold (Cladosporium fulvum), Colletotrichum (Colletotrichum), strawberry anthracnose (Colletotrichum fragariae), Puccinia (Puccinia), wheat rust (Pucci) nia recondita), Septoria spp.
  • Septoria nodorum For example, Septoria nodorum, Sclerotinia, Sclerotinia sclerotiorum, Pythium, Pythium dwarf, etc. ebaryanum Hesse), Geumanomyces ( Bacteria of the genus Gaeumannomyces, such as Gaeumannomyces graminis, and bacteria such as Peudomonas syringae, but are not limited to these examples.
  • the plant growth regulator and/or environmental stress tolerance imparting agent and/or plant disease control agent according to the present invention can be applied directly as is or after being diluted with water or the like.
  • the method of application as a chemical is not particularly limited, and includes, for example, directly attaching it to crops or seeds by spraying or dipping, spraying it on the soil, irrigating the soil, or using water or fertilizer applied to crops or soil. Examples include methods of adding it, and methods of treating it to agricultural machinery. Among these, the method of directly spraying on crops is more suitable.
  • the plant growth regulator and/or environmental stress tolerance imparting agent and/or plant disease control agent according to the present invention can be present on the plant body such as roots, stems, leaves, and seeds, or in the cultivation soil thereof. It regulates plant growth, increases yield by imparting resistance to environmental stress, and suppresses plant diseases.
  • the amount of the chemical applied in the present invention varies depending on the target crop, the target disease, the application method, the tendency of occurrence, the degree of damage, the environmental conditions, the dosage form used, etc., so it is preferable to adjust it appropriately, but it cannot be unconditionally defined.
  • a method can be exemplified in which 10 ml to 1 L, preferably 50 ml to 1 L of the solution is applied to each plant.
  • the timing of application also varies depending on the target disease, dosage form, etc., but it is preferable to apply the treatment appropriately within two weeks before and after planting.
  • the present invention is a microbial material that does not have to worry about the emergence of resistant plant pathogenic bacteria, it can be used continuously for several days or in continuous crops.
  • the plant growth regulator and/or environmental stress tolerance imparting agent and/or plant disease control agent according to the present invention may be used with other fertilizers, agricultural chemicals, such as fungicides, antiviral agents, insecticides, and pesticides, as necessary. It can be used in combination with acaricides, nematicides, synergists, attractants, herbicides, plant growth regulators, etc. In this case, they may be applied as a mixture under conditions that do not significantly affect the bacterial strain that is the active ingredient, or they may be applied separately or at the same time.
  • fungicides fungicidal active ingredients
  • disease control compounds that may be used in combination or in combination are illustrated below, but the invention is not limited to these examples.
  • Fungicidal active ingredient or disease control compound Agrobacterium radiobacter, azaconazole, acibenzolar-S-methyl, azoxystrobin, ani lazine), amisulbrom, aminopyrifene ( aminopyrifen), ametoctradin, aldimorph, isotianil, isopyrazam, isofetamide, isoflucypram ), isoprothiolane, ipconazole, ipflufenoquine ( ipflufenoquin), ipfentrifluconazole, iprodione, iprovalicarb, iprobenfos, imazalil, imi iminoctadine-albesilate, iminoctadine-triacetate , imibenconazole, impyrfluxam, imprimatin A, imprimatin B, edifenphos, etaconazol e), ethaboxam, ethyrimol (et
  • insecticides insecticidal active ingredients
  • acaricides acaricidal active ingredients
  • nematicides nematicidal active ingredients
  • synergist compounds synergist compounds
  • Herbicide compound or herbicidal active ingredient ioxynil (including lithium salt, sodium salt, salt with octanoic acid, etc.), aclonifen, acrolein, azafenidine, acifluorfen (including salt with sodium, etc.) ), azimsulfuron, asulam, acetochlor, atrazine, anisiflupurin, anilofos, amicarbazon e), amidosulfuron, amitrol (amitrole), aminocyclopyrachlor, aminopyralid, amiprofos-methyl, ametryn, Araujia Mosaic Virus), alachlor, Alternaria destruens destruens), alloxydim (including salts with sodium etc.), ancymidol, isouron, isoxachlortole, isoxaflutole, isoxaben , Isodecyl alcohol ethoxylate, isoproturon, ipfenc
  • N4-(2,6-difluorophenyl)-6-(1-fluoro- 1-Methyl-ethyl)-1,3,5-triazine-2,4-diamine N4-(2,6-difluorophenyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5 -triazine-2,4-diamine
  • chemical name, CAS registration number: 1606999-43-2 International Publication No. 2014/064094, International Publication No.
  • AE-F-150944 Code number
  • F9960 Code number
  • IR-6396 Code number
  • MCPA-thioethyl NC-656
  • SYP-298 code number
  • SYP-300 code number
  • S-ethyldipropylthiocarbamate EPTC
  • S-metolachlor S-metolachlor
  • S-9750 code number
  • MSMA MSMA
  • HW-02 code number
  • S-523 code number
  • SL-1201 code number
  • Plant growth regulator 1-naphthylacetamide, 1-methylcyclopropene, 1,3-diphenylurea, 2,3,5-triiodobenzoic acid -triiodobenzoic acid), 2-methyl-4-chlorophenoxybutyric acid (MCPB) [including sodium salt, ethyl ester, etc.], 2-(naphthalene-1-yl)acetamide (2-(naphthalene-1-yl)acetamide) , 2,6-diisopropylnaphthalene, 3-[(6-chloro-4-phenylquinazolin-2-yl)amino]propan-1-ol (3-[(6-chloro-4- phenylquinazoline-2-yl)amino]propane-1-ol), 4-oxo-4-(2-phenylethyl)aminobutyric acid (chemical name, CAS registration number: 1083-55-2), 4-chlorophenoxyacetic acid
  • Biological pesticides Haplothrips brevitubus, Franklinothrips vespiformis, Diglyphus isaea, Encarsia f. ormosa), Amlyseius cucumeris, Pseudaphycus malinus, Woolly mite (Amblyseius womersleyi), Aphidius colemani, Desert wasp (Eretmocerus eremicus), Aphidoletes aphidimyza, Swarski's cabbage mite ( Amblyseius swirskii), Orius strigicollis, Phytoseius persimilis ), Amblyseius degenerans, Phytoseius persimilis, Orius sauteri, Dacnusa sibirica, Dacnusa sibirica, Phytoseius persimilis (Amblyseius californicus), Chrysoperla nippon
  • Agricultural materials Ethylene, hypochlorous acid water (limited to those obtained by electrolyzing hydrochloric acid or potassium chloride aqueous solution), baking soda, vinegar, humus, humic acid, fulvic acid, seaweed extract, polysaccharides, amino acids, microorganisms Materials, functional ingredients derived from animals and plants, microbial metabolites, microbial activation materials, soil spreading agents, soil permeability regulating materials, soil water retention materials, etc., and biostimulants.
  • Fertilizers include inorganic fertilizers and organic fertilizers, such as ammonium chloride, ammonium sulfate, ammonium nitrate, ammonium dihydrogen phosphate, ammonium urea nitrate, urea, lime nitrogen, potassium nitrate, lime superphosphate, and lime double superphosphate. , potassium dihydrogen phosphate, potassium chloride, potassium sulfate, potassium carbonate, potassium silicate, oil cake, fish meal, rice bran, bat guano, fermented chicken manure, etc.
  • the plant growth regulator and/or environmental stress tolerance imparting agent and/or plant disease control agent of the present invention can be used to improve pest resistance, disease resistance, and weed control through new breeding techniques such as genetic recombination and genome editing, and artificial breeding. It can also be used for plants that have acquired characteristics such as chemical resistance.
  • the plant growth regulating agent of the present invention containing live bacteria or a culture of a strain belonging to the genus Bacillus as an active ingredient has a plant growth regulating effect, increases yield by imparting resistance to environmental stress, and suppresses yield loss due to disease control. It is possible to provide materials that contribute to crop production in both aspects.
  • Bacillus sp. D747 strain (FERM BP-82 34) isolated from the air in Shizuoka Prefecture was cultured on a flat plate medium, the isolated colony was inoculated into a flask, and 20 ml of bouillon medium (meat extract 1%, After culturing with shaking at 27°C and 120 rpm for 1 day in 1% peptone, 0.5% sodium chloride), the resulting culture solution was incubated with 1% glucose, 2% soluble starch, 0.5% polypeptone, and 1% dry yeast.
  • Weight to control ratio (weight of treated area/weight of control area) x 100
  • Soybean (variety: Fukuyutaka) was grown in a 128-hole cell tray filled with Yosaku (registered trademark, manufactured by J-Cam Agri Co., Ltd.), a seedling raising soil, until the first leaves developed. Then, 2.6 ml of a 500-fold diluted solution of the hydrating powder produced in accordance with Example 3 with water per soybean plant was irrigated onto the base of the soybean plant. Soybeans for comparison were irrigated with the same amount of water (control group). The next day after the irrigation treatment, the plants were transplanted into plastic cups (Tomei Chemical Industry Co., Ltd. T-600) filled with soil.
  • plastic cups Tomei Chemical Industry Co., Ltd. T-600
  • Sugar beet (variety: Papirica) is filled with Yosaku (registered trademark, manufactured by J-Cam Agri Co., Ltd.), a seedling raising soil, in paper pots for sugar beet (each size is 19 mm in diameter and 130 mm in length, bottomless and without lid). It was grown in a special tube (1,400 tubes (20 rows x 70 rows) tied together to make one book) until the four-leaf stage. Then, 0.71 ml of a 500-fold diluted solution of the hydrating powder produced in accordance with Example 3 with water per sugar beet plant was irrigated onto the base of the sugar beet plant.
  • Yosaku registered trademark, manufactured by J-Cam Agri Co., Ltd.
  • the main root of the sugar beet was crushed and filtered through double gauze, and the sugar content of the crushed liquid was measured using a digital saccharimeter.
  • the residue filtered through gauze and the crushed liquid were collected, kept in a dryer set at 120° C. for 4 days, and then weighed to determine the dry weight of the main root.
  • the weight to control ratio was calculated using equation 1.
  • the test results are shown in Table 4 and FIG. 4 below.
  • the dry weight of the taproot was 5.12 g/plant, and the sugar content of the taproot was 14.6%.
  • the taproot dry weight was 4.14 g/plant and the taproot sugar content was 13.3%, and the taproot dry weight and taproot sugar content significantly decreased under high temperature conditions.
  • the dry weight of the taproot in the normal temperature area is 5.36g/plant, and the sugar content of the taproot is 15.5%
  • the dry weight of the taproot in the high temperature area is 4.78g/plant, and the sugar content of the taproot is 14.
  • Bacillus sp. D747 strain hydrating powder showed a growth-promoting effect on sugar beets, and this effect was particularly remarkable under high temperature conditions. Therefore, Bacillus sp. D747 strain hydrating powder confers resistance to high temperature stress. it was thought.
  • Sugar beet (variety: Papirica) is filled with Yosaku (registered trademark, manufactured by J-Cam Agri Co., Ltd.), a seedling raising soil, in paper pots for sugar beet (each size is 19 mm in diameter and 130 mm in length, bottomless and without lid). It was grown in a special tube (1,400 tubes (20 rows x 70 rows) tied together to make one book) until the four-leaf stage. Then, 0.71 ml of a 500-fold diluted solution of the hydrating powder produced in accordance with Example 3 with water per sugar beet plant was irrigated onto the base of the sugar beet plant.
  • Yosaku registered trademark, manufactured by J-Cam Agri Co., Ltd.
  • the tap root of the sugar beet was kept in a dryer set at 120°C for 3 days, and then weighed to determine the dry weight of the tap root.
  • the weight to control ratio was calculated using equation 1.
  • the weight of the above-ground parts of the cabbage was measured.
  • the underground portion was kept in a dryer set at 120° C. for 2 days and then weighed to determine the dry weight of the underground portion.
  • the weight to control ratio was calculated using equation 1.
  • the test results are shown in Table 6 and Figure 6 below.
  • the aboveground living weight was 12.81 g/plant, and the underground dry weight was 0.40 g/plant.
  • the aboveground living weight was 10.84 g/plant and the underground dry weight was 0.32 g/plant, and the above ground living weight and underground dry weight were significantly increased under water stress conditions. decreased.
  • the above-ground living weight in the normal water management area was 13.12 g/plant
  • the underground dry weight was 0.44 g/plant
  • the above-ground living weight in the water-stressed area was 12.11 g/plant
  • the underground weight was 13.12 g/plant.
  • the dry weight of each plant was 0.40 g/plant, which was higher than that of the corresponding control, and the difference was particularly remarkable in the water-stressed area. There were no pests or diseases in any of the test plots. Bacillus sp. D747 strain hydrating powder showed a growth-promoting effect on cabbage, and the effect was particularly remarkable under water stress conditions. It was thought that it would be given.
  • the test results are shown in Table 7 and FIG. 7 below.
  • the above-ground living weight was 10.43 g/plant and the underground dry weight was 0.80 g/plant
  • the above-ground living weight was 11.50 g/plant
  • the dry underground part was 0.80 g/plant.
  • the weight was 0.86 g/plant, and both the aboveground living weight and underground dry weight were higher than the control.
  • Bacillus sp. D747 strain hydrating agent showed a growth promoting effect on soybean.
  • Green peppers (variety: Kyo Hikari) were grown to the 2-leaf stage in a 128-hole cell tray filled with Yosaku (registered trademark, manufactured by J-Cam Agri Co., Ltd.), a seedling raising soil. Then, 2.6 ml of a 500-fold diluted solution of the hydrating powder prepared in accordance with Example 3 with water per green pepper plant was applied to the roots of the green pepper plants. Bell peppers for comparison were irrigated with the same amount of water (control group). The next day after the irrigation treatment, the plants were transplanted into plastic cups (Tomei Chemical Industry Co., Ltd. T-600) filled with soil.
  • plastic cups Tomei Chemical Industry Co., Ltd. T-600
  • the above-ground living weight of the green pepper was measured.
  • the underground portion was kept in a dryer set at 120° C. for 2 days and then weighed to determine the dry weight of the underground portion.
  • the weight to control ratio was calculated using equation 1.
  • the test results are shown in Table 8 and FIG. 8 below.
  • the above-ground living weight was 2.11 g/plant and the underground dry weight was 0.21 g/plant
  • the above-ground living weight was 2.40 g/plant
  • the dry underground part was 0.21 g/plant.
  • the weight was 0.24 g/plant, and both the aboveground living weight and underground dry weight were higher than the control.
  • Bacillus sp. D747 strain hydrating agent showed a growth-promoting effect on green peppers.
  • the test results are shown in Table 9 and FIG. 9 below.
  • the above-ground live weight of the control plot was 0.062 g/plant, while the above-ground live weight of the wettable powder treatment plot was 0.095 g/plant, which was higher than the control. .
  • Bacillus sp. D747 strain hydrating agent showed a growth promoting effect on rapeseed.
  • the present invention can be summarized as follows.
  • the present invention is naturally derived, safe to use, and can contribute to improving agricultural production by regulating crop growth and imparting tolerance to environmental stress, increasing yield, and simultaneously controlling plant diseases.
  • the purpose is to provide microbial materials that can be used.
  • the growth of crops can be adjusted, and in addition, yield can be increased by imparting resistance to environmental stress. It is possible to provide plant growth regulators and/or plant disease control agents that increase
  • Live bacteria or cultures of Bacillus bacteria used for disease control which was unknown to conventional knowledge, regulate crop growth and increase yield by imparting resistance to environmental stress.
  • the purpose of this project is to discover and provide previously unknown knowledge that it has an action and how to use that action.
  • accession numbers of microorganisms for which the deposit procedure has been carried out in the present invention are shown below.
  • Bacillus sp. D747 (FERM BP-8234).

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  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention a pour but de fournir un matériel microbien qui est d'origine naturelle, peut être utilisé en toute sécurité, et peut contribuer à l'amélioration de la quantité de production agricole par une augmentation du rendement en régulant la croissance de cultures et en conférant une résistance au stress environnemental, tout en empêchant simultanément une maladie de plante.　La solution selon l'invention porte sur : un agent de régulation de croissance de plante qui, en contenant des cellules viables d'une souche spécifique appartenant au genre Bacillus, ou une culture comprenant les cellules viables, en tant que principe actif, augmente le rendement en régulant la croissance de cultures et en conférant une résistance au stress environnemental ; et/ou un agent de prévention de maladie de plante et similaire.　Aucun dessin sélectionné.
PCT/JP2023/015839 2022-04-22 2023-04-20 Agent de régulation de croissance de plante contenant des cellules viables ou une culture de souche du genre bacillus en tant que principe actif, et son procédé d'utilisation WO2023204285A1 (fr)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10276579A (ja) * 1997-04-09 1998-10-20 Ee H C:Kk バチルス属微生物を用いた植物の生長促進剤および生長促進方法
JP2006124280A (ja) * 2004-10-26 2006-05-18 Kumiai Chem Ind Co Ltd 植物種子発芽率向上剤
JP2016519572A (ja) * 2013-03-15 2016-07-07 スポジェン・バイオテック・インコーポレイテッドＳｐｏｇｅｎ Ｂｉｏｔｅｃｈ Ｉｎｃ． 植物成長促進細菌及び使用方法
JP2018502111A (ja) * 2014-12-29 2018-01-25 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ バチルス・アミロリケファシエンス（ｂａｃｉｌｌｕｓ ａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ｒｔｉ３０１組成物および植物成長に利益を与えかつ植物病害を処置するための使用方法
JP2018508470A (ja) * 2014-12-29 2018-03-29 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ 植物の成長に利益を与えるために土壌殺虫剤と組み合わせて使用するための微生物組成物
JP2021522792A (ja) * 2018-05-08 2021-09-02 ローカス アグリカルチャー アイピー カンパニー エルエルシー 植物の根および免疫の健康を増進するための微生物ベースの製品

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10276579A (ja) * 1997-04-09 1998-10-20 Ee H C:Kk バチルス属微生物を用いた植物の生長促進剤および生長促進方法
JP2006124280A (ja) * 2004-10-26 2006-05-18 Kumiai Chem Ind Co Ltd 植物種子発芽率向上剤
JP2016519572A (ja) * 2013-03-15 2016-07-07 スポジェン・バイオテック・インコーポレイテッドＳｐｏｇｅｎ Ｂｉｏｔｅｃｈ Ｉｎｃ． 植物成長促進細菌及び使用方法
JP2018502111A (ja) * 2014-12-29 2018-01-25 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ バチルス・アミロリケファシエンス（ｂａｃｉｌｌｕｓ ａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ｒｔｉ３０１組成物および植物成長に利益を与えかつ植物病害を処置するための使用方法
JP2018508470A (ja) * 2014-12-29 2018-03-29 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ 植物の成長に利益を与えるために土壌殺虫剤と組み合わせて使用するための微生物組成物
JP2021522792A (ja) * 2018-05-08 2021-09-02 ローカス アグリカルチャー アイピー カンパニー エルエルシー 植物の根および免疫の健康を増進するための微生物ベースの製品

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