WO2023186084A1 - Dispositif à puce et instrument pour la détection d'acide nucléique, et leur application - Google Patents

Dispositif à puce et instrument pour la détection d'acide nucléique, et leur application Download PDF

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Publication number
WO2023186084A1
WO2023186084A1 PCT/CN2023/085437 CN2023085437W WO2023186084A1 WO 2023186084 A1 WO2023186084 A1 WO 2023186084A1 CN 2023085437 W CN2023085437 W CN 2023085437W WO 2023186084 A1 WO2023186084 A1 WO 2023186084A1
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WIPO (PCT)
Prior art keywords
chamber
sample
nucleic acid
amplification reaction
chip device
Prior art date
Application number
PCT/CN2023/085437
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English (en)
Chinese (zh)
Inventor
赵海峰
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恒泰医疗有限公司
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Publication of WO2023186084A1 publication Critical patent/WO2023186084A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Definitions

  • the pressurized chamber increases the air pressure in the chamber through thermal expansion.
  • thermal expansion generally refers to the phenomenon that the length or volume of a solid or liquid material changes due to a change in temperature when the pressure remains constant.
  • the thermal expansion may be in the form of physical thermal expansion pressurization or chemical thermal expansion pressurization.
  • the nucleic acid amplification reaction of the method is an isothermal amplification method, such as loop-mediated DNA isothermal amplification (LAMP).
  • LAMP loop-mediated DNA isothermal amplification
  • FIG. 2 is a schematic diagram of an exemplary chip device for detecting nucleic acids in samples provided by the present invention, configuring reagents and working after adding samples.
  • FIG. 2A is a schematic cross-sectional view of an exemplary chip device for nucleic acid detection in samples provided by the present invention after reagents are configured therein.
  • Figure 2B is a schematic diagram of the operation of the exemplary chip device after adding a sample.
  • the invention provides a chip device for detecting nucleic acids in samples.
  • the chip device is usually used to detect the presence and amount of nucleic acid in a sample after extracting and amplifying it.
  • the chip device for nucleic acid detection in samples provided by the present invention includes a substrate 1, a sample chamber 2 arranged vertically on the substrate, and a pressurized chamber 3 arranged parallel to the sample chamber 2, and An amplification reaction chamber 4 is provided in the chip device substrate.
  • the sample chamber 2 and the pressurizing chamber 3 are cylindrical chambers surrounded by walls perpendicular to the bottom plate.
  • the sample chamber 2 and the pressurizing chamber 3 are connected through an air flow channel 5 .
  • the sample chamber 2 is connected to the amplification reaction chamber 4 through the liquid flow channel 6 provided in the substrate below it.
  • the bottom of the sample chamber 2 has an opening communicating with the liquid flow channel 6 provided in the bottom plate.
  • the sample chamber 2 is used to accommodate the sample to be detected and to separate nucleic acids in the sample.
  • the top of the sample chamber has an openable or closable cover, such as a nut 21 as shown in FIG. 1 , which has internal threads and is matched with external threads outside the sample chamber.
  • the cover of the sample chamber is a gland, which has a handle fixedly connected to the sample chamber.
  • the chip device for nucleic acid detection in this embodiment is particularly suitable for nucleic acid extraction from samples using the direct extraction method, that is, the solution obtained after the sample contacts and reacts with the nucleic acid extraction reagent contains nucleic acids that can be used for subsequent amplification reactions.
  • Samples suitable for nucleic acid extraction and amplification using the direct extraction method include synthetic clones, in vitro transcribed RNA, plasmids, serum, plasma, urine, cotton swab eluates, sputum, alveolar lavage fluid and other samples .
  • Nucleic acid extraction reagents used to extract the above samples usually contain lysis reagents, including various surfactants such as SDS, Triton, NP-40, etc., as well as other chemical reagents such as buffers, protease inhibitors, reducing agents, etc., as well as various lysis agents.
  • lysis reagents including various surfactants such as SDS, Triton, NP-40, etc., as well as other chemical reagents such as buffers, protease inhibitors, reducing agents, etc., as well as various lysis agents.
  • the main functions of the nucleic acid extraction reagent are: (1) using detergents to destroy lipid bilayers and rupture cells; (2) dissolve proteins; (3) promote protein denaturation; (4) inhibit proteases and nucleases. active.
  • Commercially available direct-extraction nucleic acid extraction reagents include sample release reagent (model S1014) provided by China Shengxiang Biotechnology Co., Ltd.
  • the amount of gas generated in the pressurized chamber 3 is the amount of original gas (i.e., volume) in the sample chamber 2 and the pressurized chamber 3, usually excluding the volume of the nucleic acid processing solution added to the sample chamber. ) about 1-50 times, preferably about 5-20 times.
  • the air pressure in the pressurizing chamber 3 can be increased through thermal expansion, including physical thermal expansion pressurization or chemical thermal expansion pressurization.
  • a filter element for filtering undesired substances (such as acidic gas or liquid) of the gas is provided in the air flow channel 5 or at its opening with the sample chamber 2 or the pressurized chamber 3 .
  • the filter element is a filter membrane 51 disposed at the opening of the air flow channel and the pressurizing chamber 3 .
  • the filter element is a filter column disposed in the air flow channel. More preferably, the shapes of the air flow channel and the filter column are set to gradually shrink from one end of the pressurizing chamber to one end of the sample chamber. This prevents the filter column from moving in the air flow path due to gas pressure.
  • the filter element is made of filter material that can absorb acidic and/or alkaline substances.
  • the workflow of the chip device for nucleic acid detection in samples is: open the cover of the sample chamber, put the sample to be tested (such as a throat swab), and A nucleic acid separation reaction is performed in the sample preservation solution, and the nucleic acid is released into the liquid; the pressurized chamber 3 is heated, and the heating causes the separation medium 81/83 to melt, and the first reactant 83 (for example, sulfuric acid) and the second reactant 84 (for example, sodium bicarbonate) contacts and reacts, and generates gas (such as carbon dioxide).
  • the first reactant 83 for example, sulfuric acid
  • the second reactant 84 for example, sodium bicarbonate
  • the amplified nucleic acid is detected by detecting a fluorescent signal carried by the nucleic acid.
  • the primers and oligonucleotides contained in the reaction system of the amplification reaction chamber can be radioactive, fluorescent or non-radioactive detectably labeled by methods well known to those skilled in the art. Commonly used fluorescent dyes and their signal-related wavelengths are shown in Table 1 below.
  • a nucleic acid detection chip as shown in Figure 1 was prepared.
  • the thickness of substrate 1 is about 2.0mm;
  • the cavity volume of sample chamber 2 is about 1.6ml, and the inner diameter is about 7.0mm;
  • the cavity volume of booster chamber 3 is about 1.2ml, and the inner diameter is about 7.0mm.
  • the size of the cross-section of the liquid flow channel is approximately 0.4mm x 0.5mm.
  • sample release agent (Changzhou Jinmag Biotechnology Co., Ltd., Su Chang Mechanical Preparation No. 20200123) into the sample chamber.
  • the testing principle of the sample release agent is to use protein denaturants and biochemical reagents to quickly destroy the virus capsid structure in the throat swab sample, thereby causing the virus to cleave and release the nucleic acid; at the same time, the nucleic acid contained is stable
  • the agent can effectively prevent the degradation of nucleic acids, thereby achieving the function of extracting and preserving nucleic acids.
  • Applicable specimen types throat swab samples or body fluid samples.
  • the reagents provided by Beijing Biolab Technology Co., Ltd.'s SARS-CoV-2 novel coronavirus RT-LAMP kit (N gene) were used as test reagents, including SARS-CoV-2N gene RT-LAMP positive control.
  • the SARS-CoV-2N gene RT-LAMP primer mixture and 2 ⁇ LAMP MagicMix in the above kit are processed to form a freeze-dried powder and added to the amplification reaction chamber.
  • the pressurized chamber is heated to about 70°C, the paraffin melts, HCl and NaHCO react to generate CO gas .
  • the sample preservation solution is squeezed into the amplification reaction chamber through the flow channel. Reconstitute the amplification raw materials pre-placed in the amplification reaction chamber.
  • a nasopharyngeal swab without SARS nucleic acid adsorption was added to another chip as a control.
  • a nasopharyngeal swab without SARS nucleic acid adsorption was added to another chip as a control, and the remaining steps were the same as in the experimental group.
  • the instrument has a chip device receiving and motion control system for receiving the chip device and performing various treatments on the chip, including heating treatment.
  • the instrument may also have a signal detection module for detecting nucleic acid amplification products, such as a fluorescence detection system.
  • the instrument has a system for temperature controlling the nucleic acid amplification area of the chip.
  • the instrument has a nucleic acid amplification result analysis and/or output system.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif à puce pour la détection d'acide nucléique d'un échantillon, ledit dispositif étant pourvu d'un substrat, d'une chambre d'échantillon et d'une chambre de pressurisation, ainsi que d'une chambre de réaction d'amplification agencée dans le substrat. L'invention concerne en outre un instrument pour la détection d'acide nucléique d'un échantillon en utilisant le dispositif à puce, et en particulier un instrument POCT. L'invention concerne en outre un procédé de détection d'acide nucléique d'un échantillon à l'aide du dispositif à puce ou de l'instrument.
PCT/CN2023/085437 2022-03-31 2023-03-31 Dispositif à puce et instrument pour la détection d'acide nucléique, et leur application WO2023186084A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202210329941 2022-03-31
CN202210329941.4 2022-03-31

Publications (1)

Publication Number Publication Date
WO2023186084A1 true WO2023186084A1 (fr) 2023-10-05

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PCT/CN2023/085437 WO2023186084A1 (fr) 2022-03-31 2023-03-31 Dispositif à puce et instrument pour la détection d'acide nucléique, et leur application

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CN (2) CN116891800A (fr)
WO (1) WO2023186084A1 (fr)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5783148A (en) * 1994-03-14 1998-07-21 Becton Dickinson And Company Nucleic acid amplification method and apparatus
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US20060222569A1 (en) * 2003-04-25 2006-10-05 Roland Barten Device and method for the preparation of analyte comprising liquids
CN101321867A (zh) * 2006-03-24 2008-12-10 株式会社东芝 核酸检测盒及核酸检测装置
JP2015062361A (ja) * 2013-09-25 2015-04-09 積水化学工業株式会社 核酸の増幅方法、並びに、核酸増幅用マイクロチップ
JP2015062360A (ja) * 2013-09-25 2015-04-09 積水化学工業株式会社 試料の加熱方法、並びに、マイクロチップ
CN111607506A (zh) * 2020-06-11 2020-09-01 上海前瞻创新研究院有限公司 一种薄膜式核酸扩增微流控芯片及其制备和应用方法
CN113492024A (zh) * 2021-09-09 2021-10-12 中国医学科学院北京协和医院 一种带自驱动单元的微流控芯片、微流控方法及其应用
WO2021229582A1 (fr) * 2020-05-14 2021-11-18 Technion Research And Development Foundation Ltd. Dispositif, systèmes, kits et procédés de détection rapide et simple d'agents pathogènes
CN114085743A (zh) * 2021-11-11 2022-02-25 杭州天微基因科技有限公司 全自动核酸处理扩增检测方法及磁控检测试管

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5783148A (en) * 1994-03-14 1998-07-21 Becton Dickinson And Company Nucleic acid amplification method and apparatus
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US20060222569A1 (en) * 2003-04-25 2006-10-05 Roland Barten Device and method for the preparation of analyte comprising liquids
CN101321867A (zh) * 2006-03-24 2008-12-10 株式会社东芝 核酸检测盒及核酸检测装置
JP2015062361A (ja) * 2013-09-25 2015-04-09 積水化学工業株式会社 核酸の増幅方法、並びに、核酸増幅用マイクロチップ
JP2015062360A (ja) * 2013-09-25 2015-04-09 積水化学工業株式会社 試料の加熱方法、並びに、マイクロチップ
WO2021229582A1 (fr) * 2020-05-14 2021-11-18 Technion Research And Development Foundation Ltd. Dispositif, systèmes, kits et procédés de détection rapide et simple d'agents pathogènes
CN111607506A (zh) * 2020-06-11 2020-09-01 上海前瞻创新研究院有限公司 一种薄膜式核酸扩增微流控芯片及其制备和应用方法
CN113492024A (zh) * 2021-09-09 2021-10-12 中国医学科学院北京协和医院 一种带自驱动单元的微流控芯片、微流控方法及其应用
CN114085743A (zh) * 2021-11-11 2022-02-25 杭州天微基因科技有限公司 全自动核酸处理扩增检测方法及磁控检测试管

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAFAVIEH EF, MANOJ K KANAKASABAPATHY, FARHANG TARLAN, MINHAZ U AHMED, MOHAMMED ZOUROB, WASEEM ASGHAR, HADI SHAFIEE : "Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection", ACS BIOMATERIALS SCIENCE & ENGINEERING, vol. 2, no. 3, 14 March 2016 (2016-03-14), pages 278 - 294, XP055923541, DOI: 10.1021/acsbiomaterials.5b00449 *

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CN220703691U (zh) 2024-04-02

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