WO2023160091A1 - 一种纯化柱 - Google Patents

一种纯化柱 Download PDF

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Publication number
WO2023160091A1
WO2023160091A1 PCT/CN2022/135594 CN2022135594W WO2023160091A1 WO 2023160091 A1 WO2023160091 A1 WO 2023160091A1 CN 2022135594 W CN2022135594 W CN 2022135594W WO 2023160091 A1 WO2023160091 A1 WO 2023160091A1
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purification column
cylinder
support wall
film
wall
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PCT/CN2022/135594
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English (en)
French (fr)
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易初丽
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武汉医蒂生物科技有限公司
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Publication of WO2023160091A1 publication Critical patent/WO2023160091A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes

Definitions

  • the invention relates to the technical field of experimental containers, in particular to a purification column.
  • nucleic acid purification columns are often used, and in the nucleic acid extraction or purification process of the column method, nucleic acid purification columns are required.
  • a nucleic acid adsorption membrane is set at the liquid outlet of the nucleic acid purification column. When the solution is transferred into the nucleic acid purification column, the nucleic acid is adsorbed by the nucleic acid adsorption membrane, and substances other than nucleic acid pass through the nucleic acid adsorption membrane and flow out from the outlet.
  • the nucleic acid solution sample to be purified such as various cell or virus solutions
  • cell lysate is added to the test tube to lyse and rupture the cells or viruses in the sample, and the nucleic acid in the cell released into the solution, and then transfer the solution in the test tube to the nucleic acid purification column through pipettes and pipette tips and other pipetting parts, and then make the solution in the nucleic acid purification column flow down through centrifugation or negative pressure.
  • the nucleic acid in the solution is adsorbed by the nucleic acid adsorption membrane, and the substances other than nucleic acid flow away through the nucleic acid adsorption membrane, and then the nucleic acid adsorption membrane is cleaned and eluted to obtain purified nucleic acid molecules.
  • the current column nucleic acid purification process requires the use of consumables such as test tubes. At the same time, it takes a lot of time to transfer the solution in the test tube to the nucleic acid purification column. At the same time, it requires the use of equipment for transferring the solution, wasting time and cost.
  • the purpose of the present invention is to provide a purification column, which can be used as both a test tube and a purification column, and does not need to prepare test tube consumables separately, which not only saves the test tube, but also saves the time for transferring the solution from the test tube to the purification column, and saves costs and increased efficiency.
  • the present invention provides the following technical solutions:
  • a purification column comprising:
  • a cylinder the cylinder is a cylinder with an open top and a sealed bottom, the bottom of the cylinder is provided with a liquid outlet, and the liquid outlet communicates with the inner cavity of the cylinder;
  • a plug arranged on the adsorption membrane, for blocking the bottom end of the cylinder
  • the plug includes a supporting wall and a covering film connected together, the upper and lower ends of the supporting wall are open, the covering film is connected to the end surface of the supporting wall away from the adsorption film, and covers the This end is open.
  • the outer wall surface of the support wall is set in conformity with the inner surface of the barrel, and the size of the outer wall surface of the support wall is set corresponding to the size of the inner surface of the barrel.
  • the adsorption film covers the inner bottom surface of the barrel, and the adsorption film is arranged correspondingly to the inner bottom surface of the barrel.
  • the thickness of the covering film is less than 0.3mm.
  • the height of the supporting wall is 2-5mm.
  • the column is a cylindrical barrel.
  • the supporting wall is a cylindrical wall.
  • the thickness of the supporting wall is 2-4mm.
  • the supporting wall is made of plastic, silicone or nylon.
  • the material of the cover film is plastic, silica gel or nylon;
  • the adsorption membrane is a nucleic acid adsorption membrane or other purification membranes.
  • the purification column provided by the present invention seals the liquid outlet at the bottom of the column by setting a plug at the bottom of the column to prevent the liquid in the column from flowing out and form a top opening and a bottom seal
  • the container can temporarily block the solution in the purification column from flowing down, so that the purification column can be used as a test tube.
  • the cover membrane is punctured, the solution in the purification column flows down through the punctured hole in the cover membrane, so that the purification column returns to the function of the column.
  • the purification column By sealing the plug on the adsorption membrane, the purification column can be used as both a test tube and a purification column, and there is no need to prepare test tube consumables separately, which not only saves the test tube, but also saves the time for transferring the solution from the test tube to the purification column. The cost is saved and the experimental efficiency is improved.
  • the purification column of the present invention has a simple structure and is easy to manufacture and use. The existing purification column does not need to be modified in structure and can be used only by inserting a plug, thereby reducing the cost of biochemical molecule purification and improving purification efficiency.
  • Fig. 1 is the structural representation of the purification column provided by the embodiment of the present invention.
  • Fig. 2 is a schematic cross-sectional structure diagram along the axial direction of the purification column provided by the embodiment of the present invention
  • FIG. 3 is a schematic diagram of a three-dimensional structure of a plug provided by an embodiment of the present invention.
  • Fig. 4 is a schematic cross-sectional structure diagram along the axial direction of the plug provided by the embodiment of the present invention.
  • Fig. 5 is a schematic structural diagram of a cylinder provided by an embodiment of the present invention.
  • Fig. 6 is a schematic structural view of the covering film in the purification column provided by the embodiment of the present invention after being punctured;
  • Fig. 7 is a schematic cross-sectional structural diagram of Fig. 6 along the axial direction;
  • Fig. 8 is a schematic diagram of the three-dimensional structure after the covering film of the stopper provided by the embodiment of the present invention is pierced;
  • Fig. 9 is a schematic cross-sectional structural diagram of Fig. 8 along the axial direction;
  • Fig. 10 is a schematic cross-sectional structural diagram along the axial direction of a purification column provided by another embodiment of the present invention.
  • the core of the present invention is to provide a purification column, which can be used as both a test tube and a purification column, and does not need to prepare test tube consumables separately, which not only saves the test tube, but also saves the time for transferring the solution from the test tube to the purification column, and saves costs and increased efficiency.
  • the purification column of the present invention includes a column body 1 , an adsorption membrane 3 and a plug 2 .
  • the cylinder 1 is a cylinder with an open top and a sealed bottom, and a liquid outlet 101 is provided at the bottom of the cylinder, and the liquid outlet 101 communicates with the inner cavity of the cylinder for discharging the liquid in the cylinder.
  • the adsorption film 3 is laid on the inner bottom surface of the barrel, covering the liquid outlet 101 , so that the solution in the barrel passes through the adsorption film 3 and then flows out through the liquid outlet 101 .
  • the plug 2 is arranged on the adsorption membrane 3 , specifically, the plug 2 is placed on the upper surface of the adsorption membrane 3 .
  • the plug 2 is used to block the bottom end surface of the cylinder 1, so that the cylinder 1 forms a cylinder with a closed bottom.
  • the plug 2 comprises a supporting wall 202 and a covering film 201 connected together, as shown in FIGS. 3 and 4 .
  • the upper and lower ends of the support wall 202 are open, and the cover film 201 is connected to the end face of the support wall 202 away from the adsorption film 3.
  • the cover film 201 covers the opening of this end of the support wall 202, so that the cover film 201 is not pierced.
  • the cylinder 1 is isolated from the liquid outlet 101, so that the bottom end of the cylinder is closed.
  • a plug 2 is arranged at the bottom end of the column body 1 to block the liquid outlet 101 located at the bottom end of the column body 1, so as to prevent the liquid in the column body 1 from flowing out and form a container with an open top and a sealed bottom end. It plays the role of temporarily blocking the solution in the purification column from flowing down, so that the purification column can be used as a test tube.
  • the cover film 201 is punctured, as shown in FIGS. 6 to 9 , the solution in the purification column flows down through the pierced hole 2010 on the cover film 201 , so that the purification column returns to the function of the column.
  • the purification column By sealing the plug 2 on the adsorption membrane 3, the purification column can be used both as a test tube and as a purification column, and there is no need to prepare test tube consumables separately, which not only saves the test tube, but also saves the time for transferring the solution from the test tube to the purification column. Time is saved, cost is improved, and experimental efficiency is improved.
  • the purification column of the present invention has a simple structure and is easy to manufacture and use. The existing purification column can be used without structural improvement and only needs to be inserted into the plug 2, which reduces the cost of biochemical molecule purification and improves purification efficiency.
  • the outer wall surface of the support wall 202 is set in conformity with the inner surface of the cylinder body, and the size of the outer wall surface of the support wall 202 is set correspondingly to the size of the inner surface of the cylinder body, thereby improving
  • the sealing performance of the purification column when used as a test tube is improved, and the solution in the column body 1 is prevented from flowing out through the gap between the outer wall surface of the support wall 202 and the inner surface of the cylinder body.
  • the adsorption film 3 covers the inner bottom surface of the cylinder, and the adsorption film 3 is arranged correspondingly to the inner bottom surface of the cylinder.
  • the corresponding setting here refers to the outer wall of the adsorption membrane 3 and the inner side of the cylinder.
  • the walls are mated and set to be identical in shape and size.
  • the covering film 201 is a very thin film-shaped structure, and the thickness of the covering film 201 is less than 0.3mm.
  • the height of the support wall 202 is greater than 2mm, specifically 2-5mm.
  • the column body 1 is a cylindrical barrel, and correspondingly, the support wall 202 is a cylindrical wall.
  • the wall thickness of the supporting wall 202 is greater than 2 mm, specifically, the wall thickness of the supporting wall 202 is 2-4 mm.
  • the material of the support wall 202 is plastic, silicone or nylon
  • the material of the cover film 201 is plastic, silicone or nylon
  • the adsorption membrane 3 is a nucleic acid adsorption membrane or other purification membranes.
  • the covering film 201 has a thickness of 0.05 mm, which is convenient to be punctured.
  • the support wall 202 is a cylindrical structure with a wall thickness of 2.5 mm, and the height of the support wall 202 is 2.5 mm.
  • a plastic ring pad 203 is arranged on the end face of the support wall 202 in contact with the adsorption film 3, as shown in Figure 10, the plastic ring pad and the support
  • the end surface of the wall 202 is arranged correspondingly to form a flexible contact surface, and the plastic ring pad 203 can also play the function of clamping the adsorption film 3 .
  • the plastic ring pad 203 and the covering film 201 are respectively fixedly connected to two ends of the support wall 202 .
  • a plug 2 of soft material is inserted into the bottom end of the column body 1, and the plug 2 is made of a soft material so that it is closely arranged with the inner wall of the column body 1 after being inserted into the column body 1, so that the plug 2 of the column body 1 Bottom seal.
  • the column body 1 Under the situation that the covering film 201 on the stopper 2 is not pierced, the column body 1 is used as a test tube for preparing a nucleic acid solution, and under the situation that the covering film 201 on the stopper 2 is pierced, the column body 1 is used as a purification column, Therefore, it is not necessary to prepare additional test tubes during the nucleic acid purification process, which saves consumables such as test tubes, and at the same time saves the transfer time of transferring the nucleic acid solution in the test tube to the purification column, saves time, and improves the efficiency of nucleic acid purification.
  • the first feature may be in direct contact with the first feature or the first and second feature may be in direct contact with the second feature through an intermediary. touch.
  • “above”, “above” and “above” the first feature on the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature.
  • “Below”, “beneath” and “beneath” the first feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is less horizontally than the second feature.

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  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种纯化柱，包括柱体（1）、吸附膜（3）和塞子（2），柱体（1）为顶部开口底部封口的筒体，筒体的底端设置有出液口（101），出液口（101）与筒体的内腔连通；吸附膜（3）铺设于筒体的内底面，覆盖出液口（101）；塞子（2）设置于吸附膜（3）上，用于封堵筒体的底端；塞子（2）包括连接在一起的支撑壁（202）和覆盖膜（201），支撑壁（202）的上下两端开口，覆盖膜（201）连接在支撑壁（202）远离吸附膜（3）的端面上，并覆盖住支撑壁（202）的此端开口。

Description

一种纯化柱
本申请要求于2022年02月25日提交中国专利局、申请号为202220404601.9、申请名称为“一种纯化柱”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及实验容器技术领域，特别涉及一种纯化柱。
背景技术
在生物化学反应中，经常要使用纯化柱，在柱式法的核酸提取或纯化过程中，需要使用核酸纯化柱。核酸纯化柱的出液口位置设置核酸吸附膜，当溶液转放入核酸纯化柱时，核酸被核酸吸附膜吸附，核酸以外的物质通过核酸吸附膜后由出液口流出。
常规的柱式法核酸纯化，首先将待纯化的核酸溶液样品如各种细胞或病毒溶液转入试管内，再向试管中加入细胞裂解液使样品中的细胞或病毒裂解破裂，细胞内的核酸释放至溶液中，然后通过移液器和移液吸头等移液部件将试管中的溶液转移至核酸纯化柱内，再通过离心或负压的方法使核酸纯化柱内的溶液下流，在下流的过程中溶液中的核酸被核酸吸附膜吸附，核酸以外的物质则通过核酸吸附膜流走，然后对核酸吸附膜进行清洗和洗脱，得到纯化的核酸分子。目前的柱式法核酸纯化过程，需要使用试管这种耗材，同时将试管内的溶液转移到核酸纯化柱内需要浪费大量的时间，同时需要使用转移溶液的器具，浪费时间和成本。
发明内容
本发明的目的是提供一种纯化柱，既能作为试管使用又能作为纯化柱使用，不需要单独准备试管耗材，既节约了试管，又节约了溶液从试管向纯化柱转移的时间，节约了成本，提高了效率。
为实现上述目的，本发明提供如下技术方案：
一种纯化柱，包括：
柱体，所述柱体为顶部开口底部封口的筒体，所述筒体的底端设置有出液口，所述出液口与所述筒体的内腔连通；
吸附膜，铺设于所述筒体的内底面，覆盖所述出液口；
塞子，设置于所述吸附膜上，用于封堵所述筒体的底端；
所述塞子包括连接在一起的支撑壁和覆盖膜，所述支撑壁的上下两端开口，所述覆盖膜连接在所述支撑壁远离所述吸附膜的端面上，并覆盖住所述支撑壁的此端开口。
可选地，所述支撑壁的外壁面与所述筒体的内表面随型设置，所述支撑壁的外壁面的尺寸与所述筒体的内表面的尺寸对应设置。
可选地，所述吸附膜覆盖于所述筒体的内底面，所述吸附膜与述筒体的内底面对应设置。
可选地，所述覆盖膜的厚度小于0.3mm。
可选地，所述支撑壁的高度为2-5mm。
可选地，所述柱体为圆柱形筒体。
可选地，所述支撑壁为圆筒型壁。
可选地，所述支撑壁的壁厚为2-4mm。
可选地，所述支撑壁的材质为塑料、硅胶或尼龙。
可选地，所述覆盖膜的材质为塑料、硅胶或尼龙；
所述吸附膜为核酸吸附膜或其它纯化膜。
从上述技术方案可以看出，本发明提供的纯化柱，通过在柱体的底端设置塞子，封堵住位于柱体底端的出液口，避免柱体内的液体流出，形成顶端开口底端封口的容器，起到暂时阻档纯化柱内的溶液下流的作用，使纯化柱充当试管使用。当覆盖膜被刺破时，纯化柱内的溶液通过覆盖膜上的刺出的破洞下流，使纯化柱回归到柱子的功能。通过在吸附膜上封堵塞子，可使纯化柱既能作为试管使用又能作为纯化柱使用，不需要单独准备试管耗材，既节约了试管，又节约了溶液从试管向纯化柱转移的时间，节约了成本，提高了实验效率。同时，本发明的纯化柱结构简单，容易制造和使用，现有的纯化柱不需要结构改进只需要塞入塞子即可使用，使生物化学分子纯化的成本降低，纯化效率提高。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据提供的附图获得其他的附图。
图1为本发明实施例提供的纯化柱的结构示意图；
图2为本发明实施例提供的纯化柱的沿轴向的剖视结构示意图；
图3为本发明实施例提供的塞子的立体结构示意图；
图4为本发明实施例提供的塞子的沿轴向的剖视结构示意图；
图5为本发明实施例提供的柱体的结构示意图；
图6为本发明实施例提供的纯化柱内的覆盖膜被刺破后的结构示意图；
图7为图6沿轴向的剖视结构示意图；
图8为本发明实施例提供的塞子的覆盖膜被刺破后的立体结构示意图；
图9为图8沿轴向的剖视结构示意图；
图10为本发明另一实施例提供的纯化柱的沿轴向的剖视结构示意图。
其中：
1-柱体，
101-出液口，
2-塞子，
201-覆盖膜，2010-破洞，202-支撑壁，203-塑料环垫，
3-吸附膜。
具体实施方式
本发明的核心是提供一种纯化柱，既能作为试管使用又能作为纯化柱使用，不需要单独准备试管耗材，既节约了试管，又节约了溶液从试管向纯化柱转移的时间，节约了成本，提高了效率。
为了使本领域的技术人员更好地理解本发明的技术方案，下面结合附图和实施方式对本发明作进一步的详细说明。
请参阅图1至图5，本发明的纯化柱，包括柱体1、吸附膜3和塞子2。柱体1为顶部开口底部封口的筒体，所述筒体的底端设置有出液口101，出液口101与所述筒体的内腔连通，用于排出所述筒体内的液体。吸附膜3铺设于所述筒体的内底面，覆盖出液口101，使得所述筒体内的溶液通过吸附膜3后再通过出液口101流出。塞子2设置于吸附膜3上，具体的，塞子2放置于吸附膜3的上表面。
其中，塞子2用于封堵柱体1的底端面，使得柱体1形成底端封闭的筒体。塞子2包括连接在一起的支撑壁202和覆盖膜201，如图3和图4所示。支撑壁202的上下两端开口，覆盖膜201连接在支撑壁202远离吸附膜3的端面上，覆盖膜201覆盖住支撑壁202的此端开口，从而在覆盖膜201未刺破的情况下使柱体1与出液口101隔断，使得所述筒体底端封闭。
本发明的纯化柱，通过在柱体1的底端设置塞子2，封堵住位于柱体1底端的出液口101，避免柱体1内的液体流出，形成顶端开口底端封口的容器，起到暂时阻档纯化柱内的溶液下流的作用，使纯化柱充当试管使用。当覆盖膜201被刺破时，参照图6至图9所示，纯化柱内的溶液通过覆盖膜201上的刺出的破洞2010下流，使纯化柱回归到柱子的功能。通过在吸附膜3上封堵塞子2，可使纯化柱既能作为试管使用又能作为纯化柱使用，不需要单独准备试管耗材，既节约了试管，又节约了溶液从试管向纯化柱转移的时间，节约了成本，提高了实验效率。同时，本发明的纯化柱结构简单，容易制造和使用，现有的纯化柱不需要结构改进只需要塞入塞子2即可使用，使生物化学分子纯化的成本降低，提高了纯化效率。
为了实现较好的封堵效果，支撑壁202的外壁面与所述筒体的内表面随型设置，支撑壁202的外壁面的尺寸与所述筒体的内表面的尺寸对应设置，从而提高了纯化柱作为试管使用时的密闭性能，避免柱体1内的溶液通过支撑壁202的外壁面与所述筒体的内表面之间的空隙流出。
具体的，吸附膜3覆盖于所述筒体的内底面，吸附膜3与述筒体的内底面对应设置，此处的对应设置，是指吸附膜3的外侧壁与所述筒体的内侧壁配合设置，形状和尺寸均相同。
为了便于刺破，覆盖膜201是很薄的膜片状结构，覆盖膜201的厚度小于0.3mm。
为了避免刺破覆盖膜201时刺破吸附膜3，支撑壁202的高度大于2mm，具体为2-5mm。当塞子2被塞入纯化柱的柱体1内后，塞子2上端面的覆盖膜201被尖物捅破时，如图8和图9所示，支撑壁202的高度可以防止尖物刺伤塞子2下端面位置的吸附膜3。
在一具体实施例中，柱体1为圆柱形筒体，对应的，支撑壁202为圆筒型壁。
为了提高塞子2的支撑能力，支撑壁202的壁厚大于2mm，具体的，支撑壁202的壁厚为2-4mm。
进一步的，支撑壁202的材质为塑料、硅胶或尼龙，覆盖膜201的材质为塑料、硅胶或尼龙。其中，吸附膜3为核酸吸附膜或其它纯化膜。
在一具体实施例中，覆盖膜201的厚度为0.05mm，便于被刺破，支撑壁202是圆筒状结构，其壁厚为2.5mm，支撑壁202的高度为2.5mm。
为了避免支撑壁202与吸附膜3接触的端面对吸附膜3产生破坏，支撑壁202与吸附膜3接触的端面上设置塑料环垫203，如图10所示，所述塑料环垫与支撑壁202的此端端面对应设置，形成柔性接触面，同时塑料环垫203还可起到卡紧吸附膜3的功能。塑料环垫203和覆盖膜201分别固定连接在支撑壁202的两端。
本发明的纯化柱，在柱体1的底端塞入软材质的塞子2，塞子2选用软材质以便于其塞入柱体1后与柱体1的内壁紧密设置,从而将柱体1的底端密封。在塞子2上的覆盖膜201未刺破的情况下，柱体1作为试管使用，用于制备核酸溶液，在塞子2上的覆盖膜201刺破的情况下，柱体1作为纯化柱使用，从而使核酸纯化过程中不需要另外准备试管，节约了试管这种耗材，同时节约了 将试管内的核酸溶液转移至纯化柱内的转移时间，节省时间，提高了核酸纯化的效率。
在本发明中，除非另有明确的规定和限定，第一特征在第二特征“上”或“下”可以是第一和第二特征直接接触，或第一和第二特征通过中间媒介间接接触。而且，第一特征在第二特征“之上”、“上方”和“上面”可是第一特征在第二特征正上方或斜上方，或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”可以是第一特征在第二特征正下方或斜下方，或仅仅表示第一特征水平高度小于第二特征。
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。

Claims (10)

  1. 一种纯化柱，其特征在于，包括：
    柱体(1)，所述柱体(1)为顶部开口底部封口的筒体，所述筒体的底端设置有出液口(101)，所述出液口(101)与所述筒体的内腔连通；
    吸附膜(3)，铺设于所述筒体的内底面，覆盖所述出液口(101)；
    塞子(2)，设置于所述吸附膜(3)上，用于封堵所述筒体的底端；
    所述塞子(2)包括连接在一起的支撑壁(202)和覆盖膜(201)，所述支撑壁(202)的上下两端开口，所述覆盖膜(201)连接在所述支撑壁(202)远离所述吸附膜(3)的端面上，并覆盖住所述支撑壁(202)的此端开口。
  2. 根据权利要求1所述的纯化柱，其特征在于，所述支撑壁(202)的外壁面与所述筒体的内表面随型设置，所述支撑壁(202)的外壁面的尺寸与所述筒体的内表面的尺寸对应设置。
  3. 根据权利要求1所述的纯化柱，其特征在于，所述吸附膜(3)覆盖于所述筒体的内底面，所述吸附膜(3)与述筒体的内底面对应设置。
  4. 根据权利要求1所述的纯化柱，其特征在于，所述覆盖膜(201)的厚度小于0.3mm。
  5. 根据权利要求1所述的纯化柱，其特征在于，所述支撑壁(202)的高度为2-5mm。
  6. 根据权利要求1所述的纯化柱，其特征在于，所述柱体(1)为圆柱形筒体。
  7. 根据权利要求1-6任一项所述的纯化柱，其特征在于，所述支撑壁(202)为圆筒型壁。
  8. 根据权利要求1所述的纯化柱，其特征在于，所述支撑壁(202)的壁厚为2-4mm。
  9. 根据权利要求1所述的纯化柱，其特征在于，所述支撑壁(202)的材质为塑料、硅胶或尼龙。
  10. 根据权利要求1所述的纯化柱，其特征在于，所述覆盖膜(201)的材质为塑料、硅胶或尼龙；
    所述吸附膜(3)为核酸吸附膜。
PCT/CN2022/135594 2022-02-25 2022-11-30 一种纯化柱 WO2023160091A1 (zh)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070148649A1 (en) * 2003-10-21 2007-06-28 Fuji Photo Film Co., Ltd. Cartridge for nucleic acid separation and purification and method for producing the same
CN106086003A (zh) * 2016-06-17 2016-11-09 易初丽 一种核酸纯化系统
CN208414378U (zh) * 2018-03-13 2019-01-22 武汉医蒂生物科技有限公司 一种核酸提取纯化装置
WO2019174539A1 (zh) * 2018-03-13 2019-09-19 武汉医蒂生物科技有限公司 核酸提取纯化装置以及生物化学分子的提取纯化装置
CN209797999U (zh) * 2018-12-20 2019-12-17 武汉医蒂生物科技有限公司 一种生物化学分子的提取纯化装置
CN214830261U (zh) * 2021-04-23 2021-11-23 厦门大学附属第一医院 一种防污染生物组织提取仪
CN217526452U (zh) * 2022-02-25 2022-10-04 武汉医蒂生物科技有限公司 一种纯化柱

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070148649A1 (en) * 2003-10-21 2007-06-28 Fuji Photo Film Co., Ltd. Cartridge for nucleic acid separation and purification and method for producing the same
CN106086003A (zh) * 2016-06-17 2016-11-09 易初丽 一种核酸纯化系统
CN208414378U (zh) * 2018-03-13 2019-01-22 武汉医蒂生物科技有限公司 一种核酸提取纯化装置
WO2019174539A1 (zh) * 2018-03-13 2019-09-19 武汉医蒂生物科技有限公司 核酸提取纯化装置以及生物化学分子的提取纯化装置
CN209797999U (zh) * 2018-12-20 2019-12-17 武汉医蒂生物科技有限公司 一种生物化学分子的提取纯化装置
CN214830261U (zh) * 2021-04-23 2021-11-23 厦门大学附属第一医院 一种防污染生物组织提取仪
CN217526452U (zh) * 2022-02-25 2022-10-04 武汉医蒂生物科技有限公司 一种纯化柱

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