WO2023154011A3 - Compositions et méthodes pour édition génomique - Google Patents

Compositions et méthodes pour édition génomique Download PDF

Info

Publication number
WO2023154011A3
WO2023154011A3 PCT/SG2023/050068 SG2023050068W WO2023154011A3 WO 2023154011 A3 WO2023154011 A3 WO 2023154011A3 SG 2023050068 W SG2023050068 W SG 2023050068W WO 2023154011 A3 WO2023154011 A3 WO 2023154011A3
Authority
WO
WIPO (PCT)
Prior art keywords
prime
genome editing
compositions
methods
editing
Prior art date
Application number
PCT/SG2023/050068
Other languages
English (en)
Other versions
WO2023154011A2 (fr
Inventor
Haojie YU
Original Assignee
National University Of Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Of Singapore filed Critical National University Of Singapore
Publication of WO2023154011A2 publication Critical patent/WO2023154011A2/fr
Publication of WO2023154011A3 publication Critical patent/WO2023154011A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Abstract

L'invention concerne de manière générale le domaine de l'édition génomique. En particulier, la spécification concerne un polypeptide éditeur primaire pouvant réaliser une édition primaire en présence d'un ARN guide d'édition primaire (ARNpeg).
PCT/SG2023/050068 2022-02-08 2023-02-08 Compositions et méthodes pour édition génomique WO2023154011A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202201201R 2022-02-08
SG10202201201R 2022-02-08

Publications (2)

Publication Number Publication Date
WO2023154011A2 WO2023154011A2 (fr) 2023-08-17
WO2023154011A3 true WO2023154011A3 (fr) 2023-09-21

Family

ID=87565226

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2023/050068 WO2023154011A2 (fr) 2022-02-08 2023-02-08 Compositions et méthodes pour édition génomique

Country Status (1)

Country Link
WO (1) WO2023154011A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021226558A1 (fr) * 2020-05-08 2021-11-11 The Broad Institute, Inc. Méthodes et compositions d'édition simultanée des deux brins d'une séquence nucléotidique double brin cible

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021226558A1 (fr) * 2020-05-08 2021-11-11 The Broad Institute, Inc. Méthodes et compositions d'édition simultanée des deux brins d'une séquence nucléotidique double brin cible

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BÖCK DESIRÉE, ROTHGANGL TANJA, VILLIGER LUKAS, SCHMIDHEINI LUKAS, MATHIS NICHOLAS, IOANNIDI ELEONORA, KREUTZER SUSANNE, KONTARAKIS: "Treatment of a metabolic liver disease by in vivo prime editing in mice", BIORXIV, 17 August 2021 (2021-08-17), XP093093410, [retrieved on 20231020], DOI: 10.1101/2021.08.17.456632 *
GAO ZONGLIANG, HALDRUP JAKOB, RAVENDRAN SUJAN, MIKKELSEN NANNA S., MIKKELSEN JACOB GIEHM, BAK RASMUS O.: "A truncated reverse transcriptase enhances prime editing by split AAV vectors", BIORXIV, 5 November 2021 (2021-11-05), XP093043208, Retrieved from the Internet <URL:https://www.biorxiv.org/content/10.1101/2021.11.05.467423v1.full.pdf> [retrieved on 20230501], DOI: 10.1101/2021.11.05.467423 *
ZHENG CHUNWEI, LIANG SHUN-QING, LIU BIN, LIU PENGPENG, KWAN SUET-YAN, WOLFE SCOT A., XUE WEN: "Development of a flexible split prime editor using truncated reverse transcriptase", BIORXIV, 29 August 2021 (2021-08-29), XP055915218, Retrieved from the Internet <URL:https://www.biorxiv.org/content/10.1101/2021.08.26.457801v2.full.pdf> [retrieved on 20220425], DOI: 10.1101/2021.08.26.457801 *

Also Published As

Publication number Publication date
WO2023154011A2 (fr) 2023-08-17

Similar Documents

Publication Publication Date Title
WO2021226558A8 (fr) Méthodes et compositions d&#39;édition simultanée des deux brins d&#39;une séquence nucléotidique double brin cible
JPWO2021226558A5 (fr)
WO2020191153A3 (fr) Procédés et compositions pour l&#39;édition de séquences nucléotidiques
EP1963525B2 (fr) Synthese de molecules d&#39;acides nucleiques a reduction d&#39;erreurs
CN106062209B (zh) 合成性长阅读dna测序
WO2004074503A3 (fr) Methodes, kits et reactifs pour le sequençage d’acides nucleiques
DE60335543D1 (de) Zusammensetzung und verfahren zur herstellung von nukleinsäuren aus sputum
KR20130101508A (ko) 단분자 ｄｎａ로 형성되는 환상 ｄｎａ의 작성 방법
CN115943209A (zh) 激活γ-珠蛋白基因表达的方法和组合物
WO2023154011A3 (fr) Compositions et méthodes pour édition génomique
JP4284063B2 (ja) 核酸塩基配列決定方法
CN107488655A (zh) 测序文库构建中5’和3’接头连接副产物的去除方法
CN110846383A (zh) 构建mRNA文库的方法及试剂盒
KR102159008B1 (ko) 자가 증폭이 가능한 헤어핀 구조의 ｎｇｓ 라이브러리 제작용 어댑터 및 이를 이용한 ｎｇｓ 라이브러리 제조방법
DE602004017980D1 (de) Verfahren zur herstellung von dna-fragmenten durch selektive fragmentierung von nukleinsäuren sowie anwendungen davon
CN110846724A (zh) 构建mRNA链特异文库的方法及试剂盒
WO2002064774A3 (fr) Construction de plasmides permettant d&#39;obtenir une orientation voulue
JP5061346B2 (ja) Ｄｎａ断片の製造方法
WO2003020873A3 (fr) Multiplication d&#39;acides ribonucleiques
JPWO2019044820A1 (ja) ２本鎖ｄｎａ断片を製造する方法
EP4079945A1 (fr) Procédé de construction de bibliothèque de mutations géniques
JP7268352B2 (ja) アダプターダイマーの生成抑制方法
US6929914B2 (en) Method for accelerated genome walking and DNA fingerprinting
JP7485104B2 (ja) アダプターダイマーの生成抑制方法
WO2003078566A3 (fr) Sequences d&#39;adn de proteines secretees massivement a partir du tetrahymena cilie, et ses utilisations