WO2023137746A1 - Culture dish filling device - Google Patents

Culture dish filling device Download PDF

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Publication number
WO2023137746A1
WO2023137746A1 PCT/CN2022/073435 CN2022073435W WO2023137746A1 WO 2023137746 A1 WO2023137746 A1 WO 2023137746A1 CN 2022073435 W CN2022073435 W CN 2022073435W WO 2023137746 A1 WO2023137746 A1 WO 2023137746A1
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WO
WIPO (PCT)
Prior art keywords
petri dish
turntable
storage
dish
culture
Prior art date
Application number
PCT/CN2022/073435
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French (fr)
Chinese (zh)
Inventor
张智彧
司同
庞任维
Original Assignee
深圳先进技术研究院
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Publication date
Application filed by 深圳先进技术研究院 filed Critical 深圳先进技术研究院
Priority to PCT/CN2022/073435 priority Critical patent/WO2023137746A1/en
Publication of WO2023137746A1 publication Critical patent/WO2023137746A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/22Petri dishes

Definitions

  • the invention relates to the technical field of microbial experiment equipment, in particular, to a petri dish filling equipment.
  • Culture medium refers to the nutrient matrix prepared by different combinations of nutrients for the growth and reproduction of microorganisms, plants or animals (or tissues). According to the physical state, it can be divided into solid medium, liquid medium, and semi-solid medium. It is usually subpackaged when used. The solidification point of the agar in the solid medium is about 40°C. Therefore, it is necessary to fill the sterilized solid medium into a petri dish at 50°C-55°C (before it solidifies). In the manual experiment operation, the experimental technicians need to measure a certain amount of medium, and complete the operations of opening, filling, covering and stacking the petri dish in sequence, which is time-consuming and laborious. The difference in the filling amount of personnel will also lead to inconsistent thickness of the flat petri dish. Since the filling needs to ensure a sterile environment, the experiment needs to be carried out in a narrow ultra-clean workbench, which also greatly reduces the preparation efficiency of the flat petri dish.
  • the purpose of the present invention is to provide a petri dish filling equipment to solve the technical problem that the current automatic filling equipment cannot automatically fill square petri dishes.
  • the invention provides a petri dish filling equipment, comprising: an equipment rack; a filling mechanism installed on the equipment rack, and the filling mechanism has a discharge pipe; a storage mechanism, installed on the equipment rack, on which a plurality of culture dishes to be filled are stored; a moving mechanism, installed on the equipment rack, can transfer a plurality of the culture dishes to the bottom of the discharge pipe one by one, and the discharge pipe can be moved to a plurality of holes of the culture dish for filling.
  • the culture dish filling equipment of the present invention transfers a plurality of culture dishes stored on the storage mechanism to the bottom of the discharge pipe of the filling mechanism one by one through the moving mechanism, and then by setting the movable discharge pipe, the discharge pipe is moved to a plurality of holes of the square culture dish, and then the liquid medium is filled to each hole, thereby realizing the automatic filling of the square culture dish.
  • Fig. 1 is a structural schematic diagram of the culture dish filling equipment of the present invention.
  • Fig. 2 is a structural schematic diagram of the filling mechanism of the present invention.
  • Fig. 3 is a structural schematic diagram of the moving mechanism of the present invention.
  • Fig. 4 is a schematic structural diagram of the first turntable of the present invention.
  • Fig. 5 is a schematic structural view of the fixed mounting plate of the present invention.
  • Fig. 6 is a schematic structural view of the storage mechanism of the present invention.
  • Fig. 7 is a structural schematic diagram of the third clamping structure and the second pushing structure of the present invention.
  • Fig. 8 is a schematic structural diagram of the equipment rack of the present invention.
  • the present invention provides a kind of petri dish filling equipment, comprising: equipment rack 1; Filling mechanism 2 is installed on the equipment rack 1, and filling mechanism 2 has discharge pipe 203; Filling is performed at a plurality of hole positions 501 .
  • the plurality of culture dishes 5 stored on the storage mechanism 4 are transferred to the bottom of the discharge pipe 203 of the filling mechanism 2 one by one through the moving mechanism 3, and then the discharge pipe 203 is moved to a plurality of hole positions 501 of the culture dish 5 by setting a movable discharge pipe 203, and then the liquid medium is filled to each hole position 501, thereby realizing automatic filling of the culture dish 5.
  • the petri dish 5 can be different types of square petri dishes, such as single-hole type, 4-hole type, 8-hole type, 24-hole type, etc., all of which can be automatically filled by the petri dish filling equipment of the present invention.
  • the petri dish is a petri dish of other shapes such as a circle.
  • the number of discharge pipes 203 of the filling mechanism 2 is one, and one discharge pipe 203 is sequentially moved to each hole 501 of the culture dish 5 for filling.
  • multiple discharge pipes can be set, and the multiple discharge pipes can be simultaneously moved to multiple holes of the culture dish for filling.
  • the discharge pipe 203 can be moved along the length direction L and the width direction W of the culture dish 5 .
  • a plurality of holes 501 in the culture dish 5 are arranged at intervals along its length direction L and width direction W, therefore, by moving the discharge pipe 203 along the length direction L and width direction W of the culture dish 5, the discharge pipe 203 can be moved to a plurality of holes 501 of various types of culture dishes 5.
  • the filling mechanism 2 includes: a transverse movement structure 201 installed on the equipment rack 1; a longitudinal movement structure 202, which is slidably matched with the transverse movement structure 201 along the length direction L of the culture dish 5;
  • the transverse movement structure 201 drives the longitudinal movement structure 202 to slide along the length direction L of the culture dish 5.
  • the transverse movement structure 201 includes a transverse movement motor 2011 and a transverse sliding seat 2013.
  • the transverse movement motor 2011 is installed on the equipment rack 1.
  • the transverse movement motor 2011 can drive the transverse movement seat 2013 to slide along the length direction L of the culture dish 5.
  • the longitudinal movement structure 202 also includes a longitudinal movement motor 2021 and a longitudinal slide rail 2022, the longitudinal movement motor 2021 and the longitudinal slide rail 2022 are all installed on the transverse sliding seat 2013, and the longitudinal sliding seat 2023 is slidably matched with the longitudinal slide rail 2022 along the width direction W of the culture dish 5.
  • the longitudinal movement structure is installed on the equipment frame, and the transverse movement structure is slidingly matched with the longitudinal movement structure along the width direction of the culture dish.
  • the transverse movement structure 201 includes a transverse slide rail 2012, and the longitudinal movement structure 202 is slidably matched with the transverse slide rail 2012 along the length direction L of the culture dish 5.
  • a waste trough 204 is provided on one side of the transverse slide rail 2012, and the longitudinal movement structure 202 can drive the discharge pipe 203 to move to the top of the waste trough 204.
  • the discharge pipe 203 can rinse the discharge pipe 203 and discharge the rinsed waste liquid into the waste tank 204 before filling the petri dish 5, and also discharge the remaining feed liquid in the discharge pipe 203 to the waste tank 204 after filling.
  • the moving mechanism 3 includes a first turntable 301 on which at least one petri dish placement slot 302 is provided, and the first turntable 301 can drive at least one petri dish placement slot 302 to move to the bottom of the storage mechanism 4 .
  • the petri dish 5 on the storage mechanism 4 is first transferred to the petri dish placement tank 302 , and then the petri dish 5 is driven by the first turntable 301 to be transferred to the bottom of the discharge pipe 203 so that the filling mechanism 2 can fill the petri dish 5 .
  • the petri dish placement groove 302 has the same shape as the petri dish 5 , and the size of the petri dish placement groove 302 is slightly larger than that of the petri dish 5 .
  • moving mechanism 3 comprises second turntable 303, and second turntable 303 is positioned at the top of first turntable 301, and second turntable 303 and first turntable 301 rotate synchronously and set, and second turntable 303 is provided with at least one petri dish through hole 304, and petri dish 5 is covered with petri dish lid 6, and petri dish 5 to be filled passes through petri dish through hole 304 and is placed in petri dish placement groove 302, and petri dish lid 6 Intercepted by the second turntable 303 and placed on the second turntable 303, the discharge pipe 203 extends from between the first turntable 301 and the second turntable 303 to the top of the culture dish 5 to be filled.
  • the petri dish 5 is covered with the petri dish cover 6 before filling, so as to avoid the petri dish 5 being polluted before filling, and the second turntable 303 can transfer the petri dish cover 6 and the petri dish 5 on the first turntable 301 to the side of the filling mechanism 2 synchronously, so that when the petri dish 5 is filled between the first turntable 301 and the second turntable 303, the discharge pipe 203 will The top of 5 is covered with a petri dish cover 6, which still has a certain protective effect on the petri dish 5 during the filling process.
  • the petri dish 5 and the petri dish cover 6 can be re-covered, thereby preventing the petri dish 5 from being polluted after canning.
  • the petri dishes 5 stored on the storage mechanism 4 are all petri dishes 5 with a petri dish cover 6, that is, petri dishes with lids, so as to ensure that the petri dishes 5 will not be polluted during storage.
  • the peripheral dimension of the petri dish cover 6 is greater than the peripheral dimension of the petri dish 5, and the size of the petri dish through hole 304 is greater than the peripheral dimension of the petri dish 5 but less than the peripheral dimension of the petri dish cap 6, so that the petri dish cap 6 cannot pass through the petri dish through the hole 304 and is intercepted by the second turntable 303, thereby being placed on the second turntable 303, while the petri dish 5 can pass through the petri dish through the hole 304 and fall into the petri dish placement groove 302 of the first turntable 301 below.
  • the second turntable 303 and the first turntable 301 are disc structures with the same radial dimension.
  • the second turntable 303 is mounted on the first turntable 301 through a plurality of support columns 312 arranged at intervals along its circumference.
  • the second turntable 303 is provided with at least one first clamping structure 305, and the first clamping structure 305 can clamp the culture dish cover 6 under the state that the culture dish 5 is separated from the culture dish cover 6 and loosen the culture dish cover 6 under the state that the culture dish 5 and the culture dish cover 6 are covered again.
  • the petri dish cover 6 is clamped by setting a first clamping structure 305 on the second turntable 303 .
  • the first clamping structure 305 includes two clamping plates 3051, and the two clamping plates 3051 are located on both sides of the culture dish passing hole 304.
  • the two clamping plates 3051 can move toward each other to clamp the culture dish cover 6.
  • the equipment rack 1 is provided with a first pushing structure 306, and the first pushing structure 306 can push the two clamping plates 3051 to move away from each other to release the culture dish cover 6.
  • the first pushing structure 306 pushes the two clamping plates 3051 to move away from each other, so that the two clamping plates 3051 move away from the top of the petri dish passing hole 304, and then when the petri dish with a cover moves down, the petri dish cover 6 is intercepted by the second turntable 303, and the petri dish 5 then passes through the petri dish passing hole 304 and falls into the petri dish placement groove 302, and then the first pushing structure 306 is lifted from the two splints 30 51, so that the two splints 3051 clamp the petri dish cover 6.
  • the first push structure 306 pushes the two splints 3051 away again, so that the petri dish cover 6 can be moved upwards synchronously with the filled petri dish 5.
  • the first push structure 306 includes a first push motor 3061 and a first push rod 3062 , the first push motor 3061 is installed on the equipment rack 1 , and the first push motor 3061 can drive the first push rod 3062 to move along the radial direction of the second turntable 303 .
  • the two splints 3051 are provided with a pressure block 3052, and the pressure block 3052 has a pressure slope 3053.
  • the pressure slope 3053 is gradually inclined toward the two sides of the culture dish through the hole 304 along the radially outward direction of the second turntable 303.
  • the first push rod 3062 has two pushing ends arranged at intervals. When the first push rod 3062 moves inward along the radial direction of the second turntable 303 and abuts against the two pressure-receiving blocks 3052, the two pressure-receiving blocks 3052 drive the two splints 3051 to move away from each other under the extrusion of the two pushing inclined surfaces 3063;
  • the first clamping structure may also adopt other clamping structures such as a clamping cylinder.
  • At least three petri dish placement slots 302 are provided on the first turntable 301 , and at least three petri dish placement slots 302 are arranged at intervals along the circumference of the first turntable 301 .
  • the feeding operation, filling operation and material return operation of different petri dish 5 can be performed at the same time, that is, at least one petri dish placement groove 302 moves to the below of the storage mechanism 4, so that a petri dish 5 falls into the petri dish placement groove 302, thereby completing the feeding operation of a petri dish 5;
  • the first turntable 301 is provided with three petri dish placement slots 302
  • the second turntable 303 is provided with corresponding three petri dish passage holes 304 .
  • the three first clamping structures 305 are arranged at intervals along the circumference of the second turntable 303
  • the second turntable 303 drives the three first clamping structures 305 to move to the positions of feeding operation, filling operation and unloading operation respectively.
  • the two first pushing structures 306 are installed on the equipment rack 1 and correspond to the positions of the feeding operation and the discharging operation respectively.
  • One of the first pushing structures 306 is used to push the first clamping structure 305 at the position of the feeding operation to be released, so that the petri dish with a cover is separated during feeding; the other first pushing structure 306 is used to promote the first clamping structure 305 at the position of the unloading operation to be released, so that the re-covered petri dish 5 and the petri dish cover 6 can be withdrawn synchronously; The petri dish cover 6 is clamped all the time in the process of moving to the position of the material return operation.
  • the moving mechanism 3 is installed on the equipment frame 1 through a fixed mounting plate 307.
  • the fixed mounting plate 307 is provided with at least one feed lifting structure 308.
  • the first turntable 301 is installed on the fixed mounting plate 307.
  • the first turntable 301 can drive at least one petri dish placing groove 302 to move to the top of at least one feeding lifting structure 308.
  • the feeding lifting structure 308 can drive the petri dish 5 to be filled on the storage mechanism 4 to drop into the petri dish placing groove 302.
  • the feeding lifting structure 308 includes a feeding lifting motor 3082 and a feeding lifting platform 3081.
  • the feeding lifting motor 3082 is installed on the fixed mounting plate 307, and the feeding lifting motor 3082 drives the feeding lifting platform 3081 to rise or fall.
  • the bottom surface of the lift culture dish placement tank 302 is provided with a lift pass hole, and the size of the lift pass hole is greater than the size of the feeding lifting platform 3081 and smaller than the size of the culture dish 5 .
  • the feeding lifting platform 3081 passes through the lifting passage hole and the petri dish passing hole 304 to rise to the bottom of the storage mechanism 4.
  • the petri dish with a cover on the storage mechanism 4 falls on the feeding lifting platform 3081, and then the petri dish with a cover is driven down by the feeding lifting platform 3081, so that the petri dish cover 6 is intercepted on the second turntable 303, and the petri dish 5 falls into the petri dish placement groove 302, thereby completing the feeding and uncapping operations of the petri dish with a cover.
  • the fixed mounting plate 307 is provided with a first turntable drive motor 310, and the first turntable 301 is connected with the first turntable drive motor 310 by a turntable shaft 311.
  • the fixed mounting plate 307 is provided with at least one lifting structure 309 for returning materials, and at least one lifting structure 308 for feeding and at least one lifting structure 309 for returning materials are located on both sides of the filling mechanism 2 .
  • the lifting structure 309 includes a lifting motor 3092 and a lifting platform 3091.
  • the lifting motor 3092 is installed on the fixed mounting plate 307, and the lifting motor 3092 drives the lifting platform 3091 to rise or fall.
  • the material return lifting platform 3091 When returning the material, the material return lifting platform 3091 first passes through the lifting passage hole to lift the petri dish 5 in the petri dish placement groove 302, and then drives the petri dish 5 to move upwards to the petri dish passing hole 304 and re-covers the petri dish cover 6, and then lifts the petri dish with a cover into the storage mechanism 4, thereby completing the closing and material removal operations of the petri dish with a lid.
  • the equipment rack 1 includes a cabinet 101 and four moving wheels 102.
  • the four moving wheels 102 are installed on the bottom of the cabinet 101.
  • the moving wheels 102 are Foma wheels, adjustable in height, and convenient to operate the entire equipment to move.
  • the filling mechanism 2, the storage mechanism 4 and the fixed mounting plate 307 are all installed on the top of the cabinet 101.
  • the first turntable drive motor 310, the feeding lifting motor 3082 and the feeding lifting motor 3092 are all located in the cabinet 101.
  • the cabinet 101 is provided with a support rod 313 for supporting the fixed mounting plate 307.
  • the storage mechanism 4 includes a storage carousel 401, on which at least one feeding storage rack 403 is arranged, and a plurality of petri dishes 5 to be filled are stacked on the feeding storage rack 403 along its height direction, and the storage carousel 401 can drive at least one feeding storage rack 403 to rotate to the top of the feeding lifting structure 308.
  • the storage carousel 401 is installed on the equipment rack 1 through the second carousel drive motor 410 , and the storage carousel 401 is partly erected above the second carousel 303 .
  • the storage carousel 401 is provided with at least one feeding through hole, and the feeding through hole is positioned at the bottom of the feeding storage rack 403.
  • the bottom of the feeding storage rack 403 is provided with a second clamping structure. Clamp down the petri dish 5 next to the bottom. When feeding is not needed, the bottom petri dish 5 in the feed storage rack 403 is clamped by the second clamping structure, so that all petri dishes 5 in the feed storage rack 403 cannot fall;
  • the size of the feeding passage hole is larger than the radial size of the petri dish with a cover but smaller than the radial size of the feeding storage shelf 403 .
  • the equipment rack 1 is provided with a second push structure 407 , the second push structure 407 includes a second push motor 4071 and a second push rod 4072 , and the second push motor 4071 can drive the second push rod 4072 to move radially along the storage carousel 401 .
  • the specific structure and working principle of the second clamping structure are similar to those of the above-mentioned first clamping structure 305, and the working principle of the second pushing structure 407 pushing the second clamping structure to release is similar to the working principle of the above-mentioned first pushing structure 306 pushing the first clamping structure 305 to loosen, and will not be repeated here.
  • the third clamping structure 406 is mounted on the second push rod 4072 .
  • the second push motor 4071 drives the second push rod 4072 to push the second clamping structure inwardly along the radial direction of the storage carousel 401, so that the second clamping structure loosens the bottommost petri dish 5, while the third clamping structure 406 clamps the bottommost petri dish 5.
  • the third clamping structure 406 includes a gripper motor 4061 and two grippers 4062 , and gripper gaskets 4063 are laid on opposite inner surfaces of the two grippers 4062 to prevent the grippers 4062 from damaging the culture dish 5 .
  • the storage carousel 401 is also provided with at least one return storage rack 404, and the storage turntable 401 can drive at least one return storage rack 404 to rotate to the top of the return lifting structure 309, and the return lifting structure 309 can raise the filled culture dish 5 and place it on the return storage rack 404.
  • a plurality of storage racks 402 are arranged at intervals along its circumference on the storage carousel 401, and the plurality of storage racks 402 are detachably connected to the storage carousel 401, and the top of the storage rack 402 is provided with a handle 405 for easy operation.
  • One of the storage racks 402 is located directly above the lifting structure 309, and no culture dish 5 to be filled is placed, thereby forming a material return storage rack 404, while the other storage racks 402 are stacked with a plurality of culture dishes 5 to be filled, forming a plurality of feeding storage racks 403, and the storage turntable 401 drives the plurality of feeding storage racks 403 to rotate one by one to the top of the feeding lifting structure 308 for feeding operation.
  • the return storage rack 404 is also filled with filled petri dishes 5, and the empty feed storage rack 403 is driven by the storage turntable 401 to rotate to the top of the return lifting structure 309, and then it is transformed into a new return storage rack 404.
  • the full return storage rack 404 from the storage carousel 401 and replacing it with a new feed storage rack 403, continuous feeding of the culture dishes 5 to be filled and continuous return of the filled culture dishes 5 are realized.
  • the material feeding storage rack 403 and the returning material storage rack 404 mentioned in the present invention do not specifically refer to a storage rack 402, but refer to storage racks 402 in two usage states.
  • the storage carousel 401 is arranged with six storage racks 402 along its circumference, among which five storage racks 402 are stacked with a plurality of petri dishes 5 to be filled, which is the feeding storage rack 403, and the remaining storage rack 402 is not placed with the petri dishes 5 to be filled, which is the return storage rack 404.
  • the storage turntable 401 drives a plurality of storage racks 402 to move, so that the return storage rack 404 is located above the return lifting structure 309 and adjacent to it.
  • a feed storage rack 403 is located above the feed lifting structure 308.
  • the storage turntable 401 drives another feed storage rack 403 adjacent to it to rotate to the top of the feed lift structure 308, and the feed storage rack 403, the petri dish passing hole 304, the petri dish placement groove 302 and the feed lifting structure 308 are located on the same axis. Rotate to the top of the material lifting structure 309 to become a new material storage rack 404.
  • the storage carousel 401 is provided with at least one return material passing hole, and the return material passing hole is located below the return material storage rack 404, and the bottom of the return material storage rack 404 is provided with a fourth clamping structure, and the fourth clamping structure can clamp the bottom culture dish 5 in the return material storage rack 404.
  • the structure of the feeding passage hole is the same as that of the feeding passage hole
  • the structure of the fourth clamping structure is the same as that of the second clamping structure, which will not be repeated here.
  • a third pushing structure 409 is provided on the equipment rack 1 , and the specific structure of the third pushing structure 409 is the same as that of the second pushing structure 407 , which will not be repeated here.
  • the fourth clamping structure is pushed by the third pushing structure 409 to loosen the culture dish 5 at the bottom.
  • the storage carousel 401 is provided with at least one return buffer rack 408, the return buffer rack 408 is located below the return storage rack 404, and the bottom of the return buffer rack 408 is provided with a one-way stopper 4081, and the one-way stopper 4081 can only be opened when the return lifting structure 309 drives the filled culture dish 5 to rise to allow the filled culture dish 5 to enter the return buffer rack 408.
  • the return buffer rack 408 is erected below the storage turntable 401, and is located directly below the return passage hole, that is, the return buffer rack 408 is fixedly arranged relative to the storage turntable 401, and when the storage turntable 401 drives the empty storage rack 402 to rotate to the top of the return buffer rack 408, the storage rack 402 forms a return storage rack 404. Since the return buffer rack 408 is provided, and the one-way stopper 4081 is set at the bottom of the return buffer rack 408, the one-way stopper 4081 is similar to the structure and working principle of the existing one-way door, that is, the one-way stopper 4081 can only be flipped up and opened when the return lifting structure 309 drives the filled culture dish 5 to lift upward.
  • the one-way stopper 4081 acts as an elastic return Flip down to a horizontal state and block the bottom of the return buffer rack 408, thereby supporting the filled culture dish 5 in the return buffer rack 408 and the return storage rack 404 when there is no need to return the material. Therefore, the third push structure 409 does not need to be provided with a clamping structure to clamp the lowermost petri dish 5 in the return storage rack 404 when returning the material.
  • the fourth clamping structure only needs to fill the return storage rack 404 completely. Clamping without clamping the lowermost culture dish 5 in the material storage rack 404 after each completed returning of a filled culture dish 5 .
  • the third push structure 409 is used to promote the fourth clamping structure to be in a loose state, so that the material return lifting structure 309 can continuously push the filled culture dish 5 upwards into the material return storage rack 404 until the material return storage rack 404 is full.

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Abstract

Disclosed is a culture dish filling device, comprising: a device frame (1); a filling mechanism (2), mounted on the device frame (1), the filling mechanism (2) being provided with a discharging pipe (203); a storage mechanism (4), mounted on the device frame (1), a plurality of culture dishes (5) to be filled being stored on the storage mechanism (4); and a moving mechanism (3), mounted on the device frame (1), wherein the moving mechanism (3) can transfer the plurality of culture dishes (5) one by one to the position below the discharging pipe (203), and the discharging pipe (203) can be moved to a plurality of hole positions (501) of the culture dish (5) for filling. According to the culture dish filling device in the present invention, the plurality of culture dishes (5) stored on the storage mechanism (4) are transferred to the position below the discharging pipe (203) of the filling mechanism (2) one by one by means of the moving mechanism (3), and then the movable discharging pipe (203) is provided, so that the discharging pipe (203) is moved to a plurality of hole positions (501) of a square culture dish, and then a liquid culture medium is filled to each hole position (501), thereby achieving automatic filling of the square culture dish.

Description

培养皿灌装设备Petri dish filling equipment 技术领域technical field
本发明涉及微生物实验设备技术领域,特别的,涉及一种培养皿灌装设备。The invention relates to the technical field of microbial experiment equipment, in particular, to a petri dish filling equipment.
背景技术Background technique
培养基,是指供给微生物、植物或动物(或组织)生长繁殖的,由不同营养物质组合配制而成的营养基质,根据物理状态可分为固体培养基、液体培养基、半固体培养基,使用时通常会进行分装,其中固体培养基中的琼脂凝固点大约在40℃,因此需要对已灭菌的固体培养基在50℃-55℃下(还未凝固前)灌装到培养皿。在手工实验操作中,实验技术人员需要量取一定量的培养基,依次完成培养皿开盖、灌装、上盖及叠放包装等操作,费时费力,人员灌装量的差异也会导致平板培养皿的厚度不一致,且由于灌装需要保证无菌环境,故需要在狭小的超净工作台内进行实验,这也大大降低了平板培养皿的制备效率。Culture medium refers to the nutrient matrix prepared by different combinations of nutrients for the growth and reproduction of microorganisms, plants or animals (or tissues). According to the physical state, it can be divided into solid medium, liquid medium, and semi-solid medium. It is usually subpackaged when used. The solidification point of the agar in the solid medium is about 40°C. Therefore, it is necessary to fill the sterilized solid medium into a petri dish at 50°C-55°C (before it solidifies). In the manual experiment operation, the experimental technicians need to measure a certain amount of medium, and complete the operations of opening, filling, covering and stacking the petri dish in sequence, which is time-consuming and laborious. The difference in the filling amount of personnel will also lead to inconsistent thickness of the flat petri dish. Since the filling needs to ensure a sterile environment, the experiment needs to be carried out in a narrow ultra-clean workbench, which also greatly reduces the preparation efficiency of the flat petri dish.
对于全自动化生物实验中,对平板划线培养、平板挑克隆等实验工艺操作需要有标准的参数来保证实验的准确性,现目前已有的自动化灌装设备是应用于圆形培养皿的灌装。由于方形培养皿有许多类型(如单孔、4孔、8孔、24孔等),现有的设备无法对方形培养皿的多个孔位进行灌装。For fully automated biological experiments, standard parameters are required for experimental process operations such as plate streak culture and plate picking clones to ensure the accuracy of the experiment. Currently, the existing automatic filling equipment is applied to the filling of round petri dishes. Since the square culture dish has many types (such as single hole, 4 holes, 8 holes, 24 holes, etc.), existing equipment cannot fill the multiple hole positions of the square culture dish.
发明内容Contents of the invention
本发明的目的是提供一种培养皿灌装设备,以解决目前的自动化灌装设备无法对方形培养皿进行自动灌装的技术问题。The purpose of the present invention is to provide a petri dish filling equipment to solve the technical problem that the current automatic filling equipment cannot automatically fill square petri dishes.
本发明的上述目的可采用下列技术方案来实现:Above-mentioned purpose of the present invention can adopt following technical scheme to realize:
本发明提供一种培养皿灌装设备,包括:设备架;灌装机构,安装在所述设备架上,所述灌装机构具有出料管;储存机构,安装在所述设备架上,所述储存机构上存放有多个待灌装的培养皿;移动机构,安装在所述设备架上,所述移动机构能将多个所述培养皿逐个转移至所述出料管的下方,所述出料管可移动至所述培养皿的多个孔位处进行灌装。The invention provides a petri dish filling equipment, comprising: an equipment rack; a filling mechanism installed on the equipment rack, and the filling mechanism has a discharge pipe; a storage mechanism, installed on the equipment rack, on which a plurality of culture dishes to be filled are stored; a moving mechanism, installed on the equipment rack, can transfer a plurality of the culture dishes to the bottom of the discharge pipe one by one, and the discharge pipe can be moved to a plurality of holes of the culture dish for filling.
本发明的特点及优点是:Features and advantages of the present invention are:
本发明的培养皿灌装设备,通过移动机构将储存机构上存放的多个培养皿逐个转移 至灌装机构的出料管的下方,进而通过设置可移动的出料管,使出料管移动至方形培养皿的多个孔位处,进而将液态培养基灌装至各个孔位处,从而实现方形培养皿的自动灌装。The culture dish filling equipment of the present invention transfers a plurality of culture dishes stored on the storage mechanism to the bottom of the discharge pipe of the filling mechanism one by one through the moving mechanism, and then by setting the movable discharge pipe, the discharge pipe is moved to a plurality of holes of the square culture dish, and then the liquid medium is filled to each hole, thereby realizing the automatic filling of the square culture dish.
附图说明Description of drawings
为了更清楚地说明本发明实施例中的技术方案,下面将对实施例描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本发明的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative work.
图1为本发明的培养皿灌装设备的结构示意图。Fig. 1 is a structural schematic diagram of the culture dish filling equipment of the present invention.
图2为本发明的灌装机构的结构示意图。Fig. 2 is a structural schematic diagram of the filling mechanism of the present invention.
图3为本发明的移动机构的结构示意图。Fig. 3 is a structural schematic diagram of the moving mechanism of the present invention.
图4为本发明的第一转盘的结构示意图。Fig. 4 is a schematic structural diagram of the first turntable of the present invention.
图5为本发明的固定安装板的结构示意图。Fig. 5 is a schematic structural view of the fixed mounting plate of the present invention.
图6为本发明的储存机构的结构示意图。Fig. 6 is a schematic structural view of the storage mechanism of the present invention.
图7为本发明的第三夹持结构和第二推动结构的结构示意图。Fig. 7 is a structural schematic diagram of the third clamping structure and the second pushing structure of the present invention.
图8为本发明的设备架的结构示意图。Fig. 8 is a schematic structural diagram of the equipment rack of the present invention.
图中:In the picture:
1、设备架;101、机柜;102、移动轮;2、灌装机构;201、横向运动结构;2011、横向运动电机;2012、横向滑轨;2013、横向滑动座;202、纵向运动结构;2021、纵向运动电机;2022、纵向滑轨;2023、纵向滑动座;203、出料管;204、废料槽;3、移动机构;301、第一转盘;302、培养皿放置槽;303、第二转盘;304、培养皿通过孔;305、第一夹紧结构;3051、夹板;3052、受压块;3053、受压斜面;306、第一推动结构;3061、第一推动电机;3062、第一推杆;3063、推动斜面;307、固定安装板;308、进料升降结构;3081、进料升降台;3082、进料升降电机;309、退料升降结构;3091、退料升降台;3092、退料升降电机;310、第一转盘驱动电机;311、转盘轴;312、支撑柱;313、支撑杆;4、储存机构;401、储存转盘;402、储存架;403、进料储存架;404、退料储存架;405、把手;406、第三夹紧结构;4061、抓手电机;4062、抓手;4063、抓手垫片;407、第二推动结构;4071、第二推动电机;4072、第二推杆;408、退料缓存架;4081、单向通行挡块;409、第三推动结构;410、第二转盘驱动电机;5、培养皿;501、孔位;6、培养皿盖。1. Equipment frame; 101. Cabinet; 102. Moving wheel; 2. Filling mechanism; 201. Lateral movement structure; 2011. Lateral movement motor; 2012. Lateral slide rail; 2013. Lateral sliding seat; 202. Longitudinal movement structure; Turntable; 302, culture dish placement groove; 303, second turntable; 304, culture dish through hole; 305, first clamping structure; 3051, splint; 3052, pressure block; 3053, pressure slope; 306, first push structure; Material lifting platform; 3082, feeding lifting motor; 309, returning material lifting structure; 3091, returning material lifting platform; 3092, returning material lifting motor; 310, first turntable drive motor; 311, turntable shaft; 312, supporting column; The third clamping structure; 4061, gripper motor; 4062, gripper; 4063, gripper gasket; 407, the second push structure; 4071, the second push motor; 4072, the second push rod;
具体实施方式Detailed ways
下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
如图1、图2以及图3所示,本发明提供一种培养皿灌装设备,包括:设备架1;灌装机构2,安装在设备架1上,灌装机构2具有出料管203;储存机构4,安装在设备架1上,储存机构4上存放有多个待灌装的培养皿5;移动机构3,安装在设备架1上,移动机构3能将多个培养皿5逐个转移至出料管203的下方,出料管203可移动至培养皿5的多个孔位501处进行灌装。As shown in Fig. 1, Fig. 2 and Fig. 3, the present invention provides a kind of petri dish filling equipment, comprising: equipment rack 1; Filling mechanism 2 is installed on the equipment rack 1, and filling mechanism 2 has discharge pipe 203; Filling is performed at a plurality of hole positions 501 .
本发明的培养皿灌装设备,通过移动机构3将储存机构4上存放的多个培养皿5逐个转移至灌装机构2的出料管203的下方,进而通过设置可移动的出料管203,使出料管203移动至培养皿5的多个孔位501处,进而将液态培养基灌装至各个孔位501处,从而实现培养皿5的自动灌装。In the culture dish filling equipment of the present invention, the plurality of culture dishes 5 stored on the storage mechanism 4 are transferred to the bottom of the discharge pipe 203 of the filling mechanism 2 one by one through the moving mechanism 3, and then the discharge pipe 203 is moved to a plurality of hole positions 501 of the culture dish 5 by setting a movable discharge pipe 203, and then the liquid medium is filled to each hole position 501, thereby realizing automatic filling of the culture dish 5.
具体的,培养皿5可为不同类型的方形培养皿,如单孔型、4孔型、8孔型、24孔型等,均能通过本发明的培养皿灌装设备实现自动灌装。可选的,培养皿为圆形等其他形状的培养皿。灌装机构2的出料管203的数量为一,通过一个出料管203依次移动至培养皿5的各个孔位501进行灌装。可选的,出料管可设置为多个,通过多个出料管同时移动至培养皿的多个孔位处进行灌装。Specifically, the petri dish 5 can be different types of square petri dishes, such as single-hole type, 4-hole type, 8-hole type, 24-hole type, etc., all of which can be automatically filled by the petri dish filling equipment of the present invention. Optionally, the petri dish is a petri dish of other shapes such as a circle. The number of discharge pipes 203 of the filling mechanism 2 is one, and one discharge pipe 203 is sequentially moved to each hole 501 of the culture dish 5 for filling. Optionally, multiple discharge pipes can be set, and the multiple discharge pipes can be simultaneously moved to multiple holes of the culture dish for filling.
如图2和图4所示,本发明的实施方式中,出料管203能沿培养皿5的长度方向L和宽度方向W移动设置。培养皿5中多个孔位501沿其长度方向L和宽度方向W间隔排布,因此,通过将出料管203沿培养皿5的长度方向L和宽度方向W移动设置,便可使出料管203能够移动至各种类型的培养皿5的多个孔位501处。As shown in FIG. 2 and FIG. 4 , in the embodiment of the present invention, the discharge pipe 203 can be moved along the length direction L and the width direction W of the culture dish 5 . A plurality of holes 501 in the culture dish 5 are arranged at intervals along its length direction L and width direction W, therefore, by moving the discharge pipe 203 along the length direction L and width direction W of the culture dish 5, the discharge pipe 203 can be moved to a plurality of holes 501 of various types of culture dishes 5.
如图2和图4所示,灌装机构2包括:横向运动结构201,安装在设备架1上;纵向运动结构202,沿培养皿5的长度方向L与横向运动结构201滑动配合,纵向运动结构202具有一纵向滑动座2023,纵向滑动座2023沿培养皿5的宽度方向W滑动设置,出料管203安装在纵向滑动座2023上。通过横向运动结构201带动纵向运动结构202沿培养皿5的长度方向L滑动,具体的,横向运动结构201包括横向运动电机2011和横向滑动座2013,横向运动电机2011安装在设备架1上,该横向运动电机2011可带动横向滑动座2013沿培养皿5的长度方向L滑动。纵向运动结构202还包括纵向运动电 机2021和纵向滑轨2022,纵向运动电机2021和纵向滑轨2022均安装在横向滑动座2013上,纵向滑动座2023沿培养皿5的宽度方向W与纵向滑轨2022滑动配合。可选的,纵向运动结构安装在设备架上,横向运动结构沿培养皿的宽度方向与纵向运动结构滑动配合。As shown in Figures 2 and 4, the filling mechanism 2 includes: a transverse movement structure 201 installed on the equipment rack 1; a longitudinal movement structure 202, which is slidably matched with the transverse movement structure 201 along the length direction L of the culture dish 5; The transverse movement structure 201 drives the longitudinal movement structure 202 to slide along the length direction L of the culture dish 5. Specifically, the transverse movement structure 201 includes a transverse movement motor 2011 and a transverse sliding seat 2013. The transverse movement motor 2011 is installed on the equipment rack 1. The transverse movement motor 2011 can drive the transverse movement seat 2013 to slide along the length direction L of the culture dish 5. The longitudinal movement structure 202 also includes a longitudinal movement motor 2021 and a longitudinal slide rail 2022, the longitudinal movement motor 2021 and the longitudinal slide rail 2022 are all installed on the transverse sliding seat 2013, and the longitudinal sliding seat 2023 is slidably matched with the longitudinal slide rail 2022 along the width direction W of the culture dish 5. Optionally, the longitudinal movement structure is installed on the equipment frame, and the transverse movement structure is slidingly matched with the longitudinal movement structure along the width direction of the culture dish.
如图2和图4所示,横向运动结构201包括横向滑轨2012,纵向运动结构202沿培养皿5的长度方向L与横向滑轨2012滑动配合,横向滑轨2012的一侧设有废料槽204,纵向运动结构202可带动出料管203移动至废料槽204的上方。通过设置废料槽204,出料管203在灌装培养皿5之前,可对出料管203进行润洗并将润洗的废液排放至废料槽204中,以及在灌装结束后也可将出料管203中剩余的料液排放至废料槽204中。As shown in Figures 2 and 4, the transverse movement structure 201 includes a transverse slide rail 2012, and the longitudinal movement structure 202 is slidably matched with the transverse slide rail 2012 along the length direction L of the culture dish 5. A waste trough 204 is provided on one side of the transverse slide rail 2012, and the longitudinal movement structure 202 can drive the discharge pipe 203 to move to the top of the waste trough 204. By setting the waste tank 204, the discharge pipe 203 can rinse the discharge pipe 203 and discharge the rinsed waste liquid into the waste tank 204 before filling the petri dish 5, and also discharge the remaining feed liquid in the discharge pipe 203 to the waste tank 204 after filling.
如图1和图4所示,本发明的实施方式中,移动机构3包括第一转盘301,第一转盘301上设有至少一培养皿放置槽302,第一转盘301可带动至少一培养皿放置槽302移动至储存机构4的下方。储存机构4上的培养皿5先转移至培养皿放置槽302中,再通过第一转盘301带动该培养皿5转移至出料管203的下方,以使灌装机构2对培养皿5进行灌装。具体的,培养皿放置槽302与培养皿5的形状相同,且培养皿放置槽302的尺寸略大于培养皿5的尺寸。As shown in FIGS. 1 and 4 , in an embodiment of the present invention, the moving mechanism 3 includes a first turntable 301 on which at least one petri dish placement slot 302 is provided, and the first turntable 301 can drive at least one petri dish placement slot 302 to move to the bottom of the storage mechanism 4 . The petri dish 5 on the storage mechanism 4 is first transferred to the petri dish placement tank 302 , and then the petri dish 5 is driven by the first turntable 301 to be transferred to the bottom of the discharge pipe 203 so that the filling mechanism 2 can fill the petri dish 5 . Specifically, the petri dish placement groove 302 has the same shape as the petri dish 5 , and the size of the petri dish placement groove 302 is slightly larger than that of the petri dish 5 .
如图1、图2以及图3所示,本发明的实施方式中,移动机构3包括第二转盘303,第二转盘303位于第一转盘301的上方,且第二转盘303与第一转盘301同步转动设置,第二转盘303上设有至少一培养皿通过孔304,培养皿5上盖合有培养皿盖6,待灌装的培养皿5穿过培养皿通过孔304并置于培养皿放置槽302中,培养皿盖6被第二转盘303拦截并置于第二转盘303上,出料管203从第一转盘301和第二转盘303之间伸至待灌装的培养皿5的上方。通过设置第二转盘303并在第二转盘303上设置培养皿通过孔304,使得培养皿5在灌装前均盖合有培养皿盖6,避免培养皿5在灌装前被污染,并且第二转盘303能将培养皿盖6与第一转盘301上的培养皿5同步转移至灌装机构2的一侧,使出料管203从第一转盘301和第二转盘303之间对培养皿5进行灌装时,培养皿5的上方遮盖有培养皿盖6,在灌装过程中对培养皿5仍具有一定的防护作用,此外,灌装结束后,通过将培养皿5向上穿过培养皿通过孔304,便可使灌装完的培养皿5与培养皿盖6重新盖合,从而避免培养皿5在罐装后被污染。As shown in Fig. 1, Fig. 2 and Fig. 3, in the embodiment of the present invention, moving mechanism 3 comprises second turntable 303, and second turntable 303 is positioned at the top of first turntable 301, and second turntable 303 and first turntable 301 rotate synchronously and set, and second turntable 303 is provided with at least one petri dish through hole 304, and petri dish 5 is covered with petri dish lid 6, and petri dish 5 to be filled passes through petri dish through hole 304 and is placed in petri dish placement groove 302, and petri dish lid 6 Intercepted by the second turntable 303 and placed on the second turntable 303, the discharge pipe 203 extends from between the first turntable 301 and the second turntable 303 to the top of the culture dish 5 to be filled. By setting the second turntable 303 and setting the petri dish passing hole 304 on the second turntable 303, the petri dish 5 is covered with the petri dish cover 6 before filling, so as to avoid the petri dish 5 being polluted before filling, and the second turntable 303 can transfer the petri dish cover 6 and the petri dish 5 on the first turntable 301 to the side of the filling mechanism 2 synchronously, so that when the petri dish 5 is filled between the first turntable 301 and the second turntable 303, the discharge pipe 203 will The top of 5 is covered with a petri dish cover 6, which still has a certain protective effect on the petri dish 5 during the filling process. In addition, after the filling is completed, by passing the petri dish 5 upward through the petri dish through the hole 304, the filled petri dish 5 and the petri dish cover 6 can be re-covered, thereby preventing the petri dish 5 from being polluted after canning.
如图3和图4所示,具体的,储存机构4上存放的培养皿5均为带有培养皿盖6的培养皿5,即带盖培养皿,确保培养皿5在储存的过程中不会被污染。培养皿盖6的外周尺寸大于培养皿5的外周尺寸,而培养皿通过孔304的尺寸大于培养皿5的外周尺寸而小于培养皿盖6的外周尺寸,使得培养皿盖6无法通过该培养皿通过孔304而被第二 转盘303拦截,从而放置在第二转盘303上,而培养皿5则能穿过该培养皿通过孔304落入下方第一转盘301的培养皿放置槽302中。第二转盘303和第一转盘301为径向尺寸相同的圆盘结构。第二转盘303通过沿其周向间隔排布的多个支撑柱312安装在第一转盘301上。As shown in Figures 3 and 4, specifically, the petri dishes 5 stored on the storage mechanism 4 are all petri dishes 5 with a petri dish cover 6, that is, petri dishes with lids, so as to ensure that the petri dishes 5 will not be polluted during storage. The peripheral dimension of the petri dish cover 6 is greater than the peripheral dimension of the petri dish 5, and the size of the petri dish through hole 304 is greater than the peripheral dimension of the petri dish 5 but less than the peripheral dimension of the petri dish cap 6, so that the petri dish cap 6 cannot pass through the petri dish through the hole 304 and is intercepted by the second turntable 303, thereby being placed on the second turntable 303, while the petri dish 5 can pass through the petri dish through the hole 304 and fall into the petri dish placement groove 302 of the first turntable 301 below. The second turntable 303 and the first turntable 301 are disc structures with the same radial dimension. The second turntable 303 is mounted on the first turntable 301 through a plurality of support columns 312 arranged at intervals along its circumference.
如图3所示,本发明的实施方式中,第二转盘303上设有至少一第一夹紧结构305,第一夹紧结构305能在培养皿5与培养皿盖6分离的状态下将培养皿盖6夹紧并在培养皿5与培养皿盖6重新盖合的状态下将培养皿盖6松开。为防止培养皿盖6倾斜而从培养皿通过孔304掉落,通过在第二转盘303上设置第一夹紧结构305将培养皿盖6夹紧。As shown in Figure 3, in the embodiment of the present invention, the second turntable 303 is provided with at least one first clamping structure 305, and the first clamping structure 305 can clamp the culture dish cover 6 under the state that the culture dish 5 is separated from the culture dish cover 6 and loosen the culture dish cover 6 under the state that the culture dish 5 and the culture dish cover 6 are covered again. In order to prevent the petri dish cover 6 from tilting and falling from the petri dish through the hole 304 , the petri dish cover 6 is clamped by setting a first clamping structure 305 on the second turntable 303 .
如图3所示,第一夹紧结构305包括两个夹板3051,两个夹板3051位于培养皿通过孔304的两侧,两个夹板3051能朝靠近彼此的方向移动而将培养皿盖6夹紧,设备架1上设有第一推动结构306,第一推动结构306能推动两个夹板3051朝远离彼此的方向移动而将培养皿盖6松开。在储存机构4上的带盖培养皿向下转移至移动机构3之前,第一推动结构306推动两个夹板3051朝远离彼此的方向移动,使两个夹板3051从培养皿通过孔304的上方让开,进而使带盖培养皿下移时,培养皿盖6被第二转盘303拦截,而培养皿5则穿过培养皿通过孔304落入培养皿放置槽302中,然后再将第一推动结构306从两个夹板3051之间移开,使得两个夹板3051夹紧培养皿盖6,灌装结束后,当培养皿5向上移动至培养皿通过孔304处而与培养皿盖6重新盖合时,第一推动结构306再次将两个夹板3051推开,使培养皿盖6能随灌装完的培养皿5同步向上移出。As shown in Figure 3, the first clamping structure 305 includes two clamping plates 3051, and the two clamping plates 3051 are located on both sides of the culture dish passing hole 304. The two clamping plates 3051 can move toward each other to clamp the culture dish cover 6. The equipment rack 1 is provided with a first pushing structure 306, and the first pushing structure 306 can push the two clamping plates 3051 to move away from each other to release the culture dish cover 6. Before the petri dish with cover on the storage mechanism 4 is transferred downward to the moving mechanism 3, the first pushing structure 306 pushes the two clamping plates 3051 to move away from each other, so that the two clamping plates 3051 move away from the top of the petri dish passing hole 304, and then when the petri dish with a cover moves down, the petri dish cover 6 is intercepted by the second turntable 303, and the petri dish 5 then passes through the petri dish passing hole 304 and falls into the petri dish placement groove 302, and then the first pushing structure 306 is lifted from the two splints 30 51, so that the two splints 3051 clamp the petri dish cover 6. After filling, when the petri dish 5 moves upwards to the petri dish through the hole 304 and re-closes with the petri dish cover 6, the first push structure 306 pushes the two splints 3051 away again, so that the petri dish cover 6 can be moved upwards synchronously with the filled petri dish 5.
如图3所示,具体的,两个夹板3051均通过弹性复位件安装在第二转盘303上,自由状态下两个夹板3051之间的距离小于培养皿盖6的宽度尺寸,因此,在未受到第一推动结构306的推力时,两个夹板3051可在弹性复位件的作用下朝靠近彼此的方向移动而将培养皿盖6夹紧。第一推动结构306包括第一推动电机3061和第一推杆3062,第一推动电机3061安装在设备架1上,第一推动电机3061能带动第一推杆3062沿第二转盘303的径向移动。两个夹板3051上设有受压块3052,该受压块3052上具有受压斜面3053,该受压斜面3053沿第二转盘303的径向向外的方向逐渐朝培养皿通过孔304的两侧倾斜,第一推杆3062具有间隔排布的两个推动端,该推动端上设有与受压斜面3053相配合的推动斜面3063,当第一推动电机3061带动第一推杆3062沿第二转盘303的径向向内移动而与两个受压块3052相抵接时,两个受压块3052在两个推动斜面3063的挤压下带动两个夹板3051朝远离彼此的方向移动,当第一推动电机3061带动第一推杆3062沿第二转盘303的径向向外移动时,两个夹板3051在弹性回复力的作用下朝靠近彼此的方向移动。可选的,第一夹紧结构也可采用夹持气缸等其他夹持结构。As shown in FIG. 3 , specifically, the two splints 3051 are installed on the second turntable 303 through the elastic reset member, and the distance between the two splints 3051 in the free state is smaller than the width of the culture dish cover 6 , therefore, when not being pushed by the first push structure 306, the two splints 3051 can move toward each other under the action of the elastic reset member to clamp the culture dish cover 6 . The first push structure 306 includes a first push motor 3061 and a first push rod 3062 , the first push motor 3061 is installed on the equipment rack 1 , and the first push motor 3061 can drive the first push rod 3062 to move along the radial direction of the second turntable 303 . The two splints 3051 are provided with a pressure block 3052, and the pressure block 3052 has a pressure slope 3053. The pressure slope 3053 is gradually inclined toward the two sides of the culture dish through the hole 304 along the radially outward direction of the second turntable 303. The first push rod 3062 has two pushing ends arranged at intervals. When the first push rod 3062 moves inward along the radial direction of the second turntable 303 and abuts against the two pressure-receiving blocks 3052, the two pressure-receiving blocks 3052 drive the two splints 3051 to move away from each other under the extrusion of the two pushing inclined surfaces 3063; Optionally, the first clamping structure may also adopt other clamping structures such as a clamping cylinder.
如图4所示,本发明的实施方式中,第一转盘301上设有至少三个培养皿放置槽302,至少三个培养皿放置槽302沿第一转盘301的周向间隔排布。通过在第一转盘301上设置至少三个培养皿放置槽302,可同时进行不同培养皿5的进料操作、灌装操作以及退料操作,即至少一培养皿放置槽302移动至储存机构4的下方,使一培养皿5落入该培养皿放置槽302中,从而完成一培养皿5的进料操作;至少一培养皿放置槽302上的培养皿5移动至出料管203的下方进行灌装,从而完成另一培养皿5的灌装操作;至少一培养皿放置槽302将灌装完的培养皿5移动至出料管203的侧方,使该培养皿5与培养皿盖6重新盖合后从移动机构3上离开,从而完成又一培养皿5的退料操作,整个设备的连续性好,灌装效率更高。As shown in FIG. 4 , in an embodiment of the present invention, at least three petri dish placement slots 302 are provided on the first turntable 301 , and at least three petri dish placement slots 302 are arranged at intervals along the circumference of the first turntable 301 . By arranging at least three petri dish placement grooves 302 on the first turntable 301, the feeding operation, filling operation and material return operation of different petri dish 5 can be performed at the same time, that is, at least one petri dish placement groove 302 moves to the below of the storage mechanism 4, so that a petri dish 5 falls into the petri dish placement groove 302, thereby completing the feeding operation of a petri dish 5; Loading operation: at least one petri dish placement slot 302 moves the filled petri dish 5 to the side of the discharge pipe 203, so that the petri dish 5 and the petri dish cover 6 are re-closed and then left from the moving mechanism 3, thereby completing the material return operation of another petri dish 5, the continuity of the entire equipment is good, and the filling efficiency is higher.
具体的,如图1、图3以及图4所示,第一转盘301上设有三个培养皿放置槽302,第二转盘303上设有相对应的三个培养皿通过孔304。三个第一夹紧结构305沿第二转盘303的周向间隔排布,第二转盘303带动三个第一夹紧结构305分别移动至进料操作的位置、灌装操作的位置以及退料操作的位置。两个第一推动结构306安装在设备架1上,并分别与进料操作的位置和退料操作的位置相对应。其中一第一推动结构306用于推动进料操作的位置处的第一夹紧结构305松开,以使带盖的培养皿在进料时分离;另一第一推动结构306用于推动退料操作的位置处的第一夹紧结构305松开,以使重新盖合的培养皿5和培养皿盖6能同步退出;而第一夹紧结构305在从进料操作的位置移动至灌装操作的位置的过程中、灌装操作的过程中、以及从灌装操作的位置移动至退料操作的位置的过程中始终将培养皿盖6夹紧。Specifically, as shown in FIG. 1 , FIG. 3 and FIG. 4 , the first turntable 301 is provided with three petri dish placement slots 302 , and the second turntable 303 is provided with corresponding three petri dish passage holes 304 . The three first clamping structures 305 are arranged at intervals along the circumference of the second turntable 303 , and the second turntable 303 drives the three first clamping structures 305 to move to the positions of feeding operation, filling operation and unloading operation respectively. The two first pushing structures 306 are installed on the equipment rack 1 and correspond to the positions of the feeding operation and the discharging operation respectively. One of the first pushing structures 306 is used to push the first clamping structure 305 at the position of the feeding operation to be released, so that the petri dish with a cover is separated during feeding; the other first pushing structure 306 is used to promote the first clamping structure 305 at the position of the unloading operation to be released, so that the re-covered petri dish 5 and the petri dish cover 6 can be withdrawn synchronously; The petri dish cover 6 is clamped all the time in the process of moving to the position of the material return operation.
如图4和图5所示,移动机构3通过固定安装板307安装在设备架1上,固定安装板307上设有至少一进料升降结构308,第一转盘301安装在固定安装板307上,第一转盘301可带动至少一培养皿放置槽302移动至至少一进料升降结构308的上方,进料升降结构308可带动储存机构4上待灌装的培养皿5下降至培养皿放置槽302内。As shown in Fig. 4 and Fig. 5, the moving mechanism 3 is installed on the equipment frame 1 through a fixed mounting plate 307. The fixed mounting plate 307 is provided with at least one feed lifting structure 308. The first turntable 301 is installed on the fixed mounting plate 307. The first turntable 301 can drive at least one petri dish placing groove 302 to move to the top of at least one feeding lifting structure 308. The feeding lifting structure 308 can drive the petri dish 5 to be filled on the storage mechanism 4 to drop into the petri dish placing groove 302.
如图3、图4以及图5所示,具体的,进料升降结构308包括进料升降电机3082和进料升降台3081,进料升降电机3082安装在固定安装板307上,通过进料升降电机3082带动进料升降台3081上升或下降。升降培养皿放置槽302的槽底面上设有一升降通过孔,该升降通过孔的尺寸大于进料升降台3081的尺寸而小于培养皿5的尺寸。进料时,进料升降台3081依次穿过升降通过孔和培养皿通过孔304升至储存机构4的下方,储存机构4上的带盖培养皿落在进料升降台3081上,进而通过进料升降台3081带动带盖培养皿下移,而使培养皿盖6被拦截在第二转盘303上,培养皿5则落入培养皿放置槽302中,从而完成带盖培养皿的进料和开盖操作。固定安装板307设有第一转盘 驱动电机310,第一转盘301通过转盘轴311与第一转盘驱动电机310连接。As shown in Figure 3, Figure 4 and Figure 5, specifically, the feeding lifting structure 308 includes a feeding lifting motor 3082 and a feeding lifting platform 3081. The feeding lifting motor 3082 is installed on the fixed mounting plate 307, and the feeding lifting motor 3082 drives the feeding lifting platform 3081 to rise or fall. The bottom surface of the lift culture dish placement tank 302 is provided with a lift pass hole, and the size of the lift pass hole is greater than the size of the feeding lifting platform 3081 and smaller than the size of the culture dish 5 . When feeding, the feeding lifting platform 3081 passes through the lifting passage hole and the petri dish passing hole 304 to rise to the bottom of the storage mechanism 4. The petri dish with a cover on the storage mechanism 4 falls on the feeding lifting platform 3081, and then the petri dish with a cover is driven down by the feeding lifting platform 3081, so that the petri dish cover 6 is intercepted on the second turntable 303, and the petri dish 5 falls into the petri dish placement groove 302, thereby completing the feeding and uncapping operations of the petri dish with a cover. The fixed mounting plate 307 is provided with a first turntable drive motor 310, and the first turntable 301 is connected with the first turntable drive motor 310 by a turntable shaft 311.
固定安装板307上设有至少一退料升降结构309,且至少一进料升降结构308和至少一退料升降结构309位于灌装机构2的两侧,第一转盘301可带动至少一培养皿放置槽302移动至至少一退料升降结构309的上方,至少一退料升降结构309可带动培养皿放置槽302内灌装完的培养皿5上升至储存机构4上。具体的,退料升降结构309包括退料升降电机3092和退料升降台3091,退料升降电机3092安装在固定安装板307上,通过退料升降电机3092带动退料升降台3091上升或下降。退料时,退料升降台3091先穿过升降通过孔将培养皿放置槽302内的培养皿5顶起,进而带动培养皿5向上移动至培养皿通过孔304处与培养皿盖6重新盖合,进而将带盖培养皿升至储存机构4内,从而完成带盖培养皿的合盖和退料操作。The fixed mounting plate 307 is provided with at least one lifting structure 309 for returning materials, and at least one lifting structure 308 for feeding and at least one lifting structure 309 for returning materials are located on both sides of the filling mechanism 2 . Specifically, the lifting structure 309 includes a lifting motor 3092 and a lifting platform 3091. The lifting motor 3092 is installed on the fixed mounting plate 307, and the lifting motor 3092 drives the lifting platform 3091 to rise or fall. When returning the material, the material return lifting platform 3091 first passes through the lifting passage hole to lift the petri dish 5 in the petri dish placement groove 302, and then drives the petri dish 5 to move upwards to the petri dish passing hole 304 and re-covers the petri dish cover 6, and then lifts the petri dish with a cover into the storage mechanism 4, thereby completing the closing and material removal operations of the petri dish with a lid.
如图1和图8所示,设备架1包括机柜101和四个移动轮102,四个移动轮102安装在机柜101的底部,该移动轮102为福马轮,可调节高度,且便于操作整个设备移动。灌装机构2、储存机构4以及固定安装板307均安装在机柜101的顶部,第一转盘驱动电机310、进料升降电机3082以及退料升降电机3092均位于机柜101内,机柜101内设有用于对固定安装板307进行支撑的支撑杆313。As shown in Figures 1 and 8, the equipment rack 1 includes a cabinet 101 and four moving wheels 102. The four moving wheels 102 are installed on the bottom of the cabinet 101. The moving wheels 102 are Foma wheels, adjustable in height, and convenient to operate the entire equipment to move. The filling mechanism 2, the storage mechanism 4 and the fixed mounting plate 307 are all installed on the top of the cabinet 101. The first turntable drive motor 310, the feeding lifting motor 3082 and the feeding lifting motor 3092 are all located in the cabinet 101. The cabinet 101 is provided with a support rod 313 for supporting the fixed mounting plate 307.
如图1和图6所示,本发明的实施方式中,储存机构4包括储存转盘401,储存转盘401上设有至少一进料储存架403,进料储存架403上沿其高度方向叠放有多个待灌装的培养皿5,储存转盘401可带动至少一进料储存架403转动至进料升降结构308的上方。具体的,储存转盘401通过第二转盘驱动电机410安装在设备架1上,且储存转盘401部分架设于第二转盘303的上方。As shown in Figures 1 and 6, in the embodiment of the present invention, the storage mechanism 4 includes a storage carousel 401, on which at least one feeding storage rack 403 is arranged, and a plurality of petri dishes 5 to be filled are stacked on the feeding storage rack 403 along its height direction, and the storage carousel 401 can drive at least one feeding storage rack 403 to rotate to the top of the feeding lifting structure 308. Specifically, the storage carousel 401 is installed on the equipment rack 1 through the second carousel drive motor 410 , and the storage carousel 401 is partly erected above the second carousel 303 .
如图1、图5以及图6所示,储存转盘401上设有至少一进料通过孔,进料通过孔位于进料储存架403的下方,进料储存架403的底部设有第二夹紧结构,第二夹紧结构能在无需进料的状态下将进料储存架403内最底层的培养皿5夹紧并在进料状态下将该培养皿5松开,设备架1上设有第三夹紧结构406,第三夹紧结构406能在进料状态下将紧邻最底层的培养皿5夹紧。当无需进料时,通过第二夹紧结构将进料储存架403内最底层的培养皿5夹紧,使得进料储存架403中所有的培养皿5均无法下落,当需要进料时,则通过第三夹紧结构406将紧邻最底层的培养皿5夹紧并使第二夹紧结构将最底层的培养皿5松开,使最底层的培养皿5能落到下方的进料升降结构308上。As shown in Fig. 1, Fig. 5 and Fig. 6, the storage carousel 401 is provided with at least one feeding through hole, and the feeding through hole is positioned at the bottom of the feeding storage rack 403. The bottom of the feeding storage rack 403 is provided with a second clamping structure. Clamp down the petri dish 5 next to the bottom. When feeding is not needed, the bottom petri dish 5 in the feed storage rack 403 is clamped by the second clamping structure, so that all petri dishes 5 in the feed storage rack 403 cannot fall;
具体的,进料通过孔的尺寸大于带盖培养皿的径向尺寸而小于进料储存架403的径向尺寸。如图6和图7所示,设备架1上设有第二推动结构407,第二推动结构407包括第二推动电机4071和第二推杆4072,第二推动电机4071可带动第二推杆4072沿储 存转盘401的径向移动。第二夹紧结构与上述第一夹紧结构305的具体结构和工作原理相似,第二推动结构407推动第二夹紧结构松开的工作原理与上述第一推动结构306推动第一夹紧结构305松开的工作原理相似,在此不再赘述。第三夹紧结构406安装在第二推杆4072上。第二推动电机4071带动第二推杆4072沿储存转盘401的径向向内推动第二夹紧结构,使第二夹紧结构将最底层的培养皿5松开,同时第三夹紧结构406将紧邻最底层的培养皿5夹紧。第三夹紧结构406包括抓手电机4061和两个抓手4062,且两个抓手4062相对的内侧面上铺设有抓手垫片4063,以防止抓手4062对培养皿5造成损坏。Specifically, the size of the feeding passage hole is larger than the radial size of the petri dish with a cover but smaller than the radial size of the feeding storage shelf 403 . As shown in FIGS. 6 and 7 , the equipment rack 1 is provided with a second push structure 407 , the second push structure 407 includes a second push motor 4071 and a second push rod 4072 , and the second push motor 4071 can drive the second push rod 4072 to move radially along the storage carousel 401 . The specific structure and working principle of the second clamping structure are similar to those of the above-mentioned first clamping structure 305, and the working principle of the second pushing structure 407 pushing the second clamping structure to release is similar to the working principle of the above-mentioned first pushing structure 306 pushing the first clamping structure 305 to loosen, and will not be repeated here. The third clamping structure 406 is mounted on the second push rod 4072 . The second push motor 4071 drives the second push rod 4072 to push the second clamping structure inwardly along the radial direction of the storage carousel 401, so that the second clamping structure loosens the bottommost petri dish 5, while the third clamping structure 406 clamps the bottommost petri dish 5. The third clamping structure 406 includes a gripper motor 4061 and two grippers 4062 , and gripper gaskets 4063 are laid on opposite inner surfaces of the two grippers 4062 to prevent the grippers 4062 from damaging the culture dish 5 .
如图1、图5以及图6所示,本发明的实施方式中,储存转盘401上还设有至少一退料储存架404,储存转盘401可带动至少一退料储存架404转动至退料升降结构309的上方,退料升降结构309能将灌装完的培养皿5上升并放置于退料储存架404上。As shown in Figures 1, 5 and 6, in an embodiment of the present invention, the storage carousel 401 is also provided with at least one return storage rack 404, and the storage turntable 401 can drive at least one return storage rack 404 to rotate to the top of the return lifting structure 309, and the return lifting structure 309 can raise the filled culture dish 5 and place it on the return storage rack 404.
如图1、图5以及图6所示,具体的,储存转盘401上沿其周向间隔排布有多个储存架402,且多个储存架402与储存转盘401可拆卸连接,储存架402的顶部设有把手405,便于操作。其中一储存架402位于退料升降结构309的正上方,且未放置待灌装的培养皿5,从而构成退料储存架404,而另外的多个储存架402则叠放有多个待灌装的培养皿5,构成多个进料储存架403,通过储存转盘401带动多个进料储存架403逐个转动至进料升降结构308的上方进行进料操作。当进料储存架403上的培养皿5全部完成进料操作的同时,退料储存架404上也装满了灌装完的培养皿5,通过储存转盘401带动空的进料储存架403转动至退料升降结构309的上方,则转变成新的退料储存架404。通过将装满的退料储存架404从储存转盘401上拆下,更换上新的进料储存架403,从而实现待灌装的培养皿5的连续进料以及灌装完的培养皿5的连续退料。As shown in Figure 1, Figure 5 and Figure 6, specifically, a plurality of storage racks 402 are arranged at intervals along its circumference on the storage carousel 401, and the plurality of storage racks 402 are detachably connected to the storage carousel 401, and the top of the storage rack 402 is provided with a handle 405 for easy operation. One of the storage racks 402 is located directly above the lifting structure 309, and no culture dish 5 to be filled is placed, thereby forming a material return storage rack 404, while the other storage racks 402 are stacked with a plurality of culture dishes 5 to be filled, forming a plurality of feeding storage racks 403, and the storage turntable 401 drives the plurality of feeding storage racks 403 to rotate one by one to the top of the feeding lifting structure 308 for feeding operation. When the petri dishes 5 on the feed storage rack 403 have all completed the feed operation, the return storage rack 404 is also filled with filled petri dishes 5, and the empty feed storage rack 403 is driven by the storage turntable 401 to rotate to the top of the return lifting structure 309, and then it is transformed into a new return storage rack 404. By removing the full return storage rack 404 from the storage carousel 401 and replacing it with a new feed storage rack 403, continuous feeding of the culture dishes 5 to be filled and continuous return of the filled culture dishes 5 are realized.
由此可知,本发明中所述的进料储存架403和退料储存架404并不是特指某个储存架402,而是指两种使用状态下的储存架402。本实施例中,储存转盘401沿其周向排布有六个储存架402,其中五个储存架402上均叠放有多个待灌装的培养皿5,为进料储存架403,而剩下的一个储存架402则未放置待灌装的培养皿5,为退料储存架404,通过储存转盘401带动多个储存架402移动,使退料储存架404位于退料升降结构309的上方,而与其相邻的一进料储存架403位于进料升降结构308的上方,当该进料储存架403上的培养皿5全部完成进料后,通过储存转盘401带动与其相邻的另一进料储存架403转动至进料升降结构308的上方,且该进料储存架403、培养皿通过孔304、培养皿放置槽302以及进料升降结构308位于同一轴向上,同时全部完成进料的进料储存架403则转动至退料升降结构309的上方成为新的退料储存架404.It can be seen from this that the material feeding storage rack 403 and the returning material storage rack 404 mentioned in the present invention do not specifically refer to a storage rack 402, but refer to storage racks 402 in two usage states. In this embodiment, the storage carousel 401 is arranged with six storage racks 402 along its circumference, among which five storage racks 402 are stacked with a plurality of petri dishes 5 to be filled, which is the feeding storage rack 403, and the remaining storage rack 402 is not placed with the petri dishes 5 to be filled, which is the return storage rack 404. The storage turntable 401 drives a plurality of storage racks 402 to move, so that the return storage rack 404 is located above the return lifting structure 309 and adjacent to it. A feed storage rack 403 is located above the feed lifting structure 308. When all the petri dishes 5 on the feed storage rack 403 have been fed, the storage turntable 401 drives another feed storage rack 403 adjacent to it to rotate to the top of the feed lift structure 308, and the feed storage rack 403, the petri dish passing hole 304, the petri dish placement groove 302 and the feed lifting structure 308 are located on the same axis. Rotate to the top of the material lifting structure 309 to become a new material storage rack 404.
如图1、图5以及图6所示,本发明的实施方式中,储存转盘401上设有至少一退料通过孔,退料通过孔位于退料储存架404的下方,退料储存架404的底部设有第四夹紧结构,第四夹紧结构能将退料储存架404内最底层的培养皿5夹紧。具体的,退料通过孔与进料通过孔的结构相同,第四夹紧结构与第二夹紧结构的结构相同,在此不再赘述。设备架1上设有第三推动结构409,第三推动结构409与第二推动结构407的具体结构相同,在此不再赘述。通过第三推动结构409推动第四夹紧结构将最底层的培养皿5松开。As shown in Fig. 1, Fig. 5 and Fig. 6, in the embodiment of the present invention, the storage carousel 401 is provided with at least one return material passing hole, and the return material passing hole is located below the return material storage rack 404, and the bottom of the return material storage rack 404 is provided with a fourth clamping structure, and the fourth clamping structure can clamp the bottom culture dish 5 in the return material storage rack 404. Specifically, the structure of the feeding passage hole is the same as that of the feeding passage hole, and the structure of the fourth clamping structure is the same as that of the second clamping structure, which will not be repeated here. A third pushing structure 409 is provided on the equipment rack 1 , and the specific structure of the third pushing structure 409 is the same as that of the second pushing structure 407 , which will not be repeated here. The fourth clamping structure is pushed by the third pushing structure 409 to loosen the culture dish 5 at the bottom.
如图5和图6所示,储存转盘401上设有至少一退料缓存架408,退料缓存架408位于退料储存架404的下方,退料缓存架408的底部设有单向通行挡块4081,单向通行挡块4081仅能在退料升降结构309带动灌装完的培养皿5上升的状态下开启而使灌装完的培养皿5进入退料缓存架408内。As shown in Figures 5 and 6, the storage carousel 401 is provided with at least one return buffer rack 408, the return buffer rack 408 is located below the return storage rack 404, and the bottom of the return buffer rack 408 is provided with a one-way stopper 4081, and the one-way stopper 4081 can only be opened when the return lifting structure 309 drives the filled culture dish 5 to rise to allow the filled culture dish 5 to enter the return buffer rack 408.
具体的,退料缓存架408架设在储存转盘401的下方,且位于退料通过孔的正下方,即退料缓存架408相对于储存转盘401固定设置,当储存转盘401带动空的储存架402转动至退料缓存架408的上方时,则该储存架402形成退料储存架404。由于设置了退料缓存架408,并在退料缓存架408的底部设置单向通行挡块4081,单向通行挡块4081与现有的单向门的结构和工作原理相似,即该单向通行挡块4081只能在退料升降结构309带动灌装完的培养皿5向上顶升时而向上翻转开启,退料升降结构309下降后,单向通行挡块4081则在弹性复位件的作用下向下翻转至水平状态而挡于退料缓存架408的底部,从而在无需退料时对退料缓存架408以及退料储存架404中灌装完的培养皿5进行支撑,因此,第三推动结构409上无需设置夹紧结构在退料时将退料储存架404内紧邻最底层的培养皿5夹紧,第四夹紧结构只需在退料储存架404中全部装满后将退料储存架404内最底层的培养皿5夹紧,而无需在每完成一个灌装完的培养皿5的退料后将退料储存架404内最底层的培养皿5夹紧。当退料储存架404转动至退料缓存架408的正上方后,便通过第三推动结构409推动第四夹紧结构为松开状态,使退料升降结构309能够将灌装完的培养皿5不断地向上顶入退料储存架404中,直至退料储存架404装满后将第三推动结构409从第四夹紧结构处移开,使第四夹紧结构夹紧退料储存架404中最底层的培养皿5。Specifically, the return buffer rack 408 is erected below the storage turntable 401, and is located directly below the return passage hole, that is, the return buffer rack 408 is fixedly arranged relative to the storage turntable 401, and when the storage turntable 401 drives the empty storage rack 402 to rotate to the top of the return buffer rack 408, the storage rack 402 forms a return storage rack 404. Since the return buffer rack 408 is provided, and the one-way stopper 4081 is set at the bottom of the return buffer rack 408, the one-way stopper 4081 is similar to the structure and working principle of the existing one-way door, that is, the one-way stopper 4081 can only be flipped up and opened when the return lifting structure 309 drives the filled culture dish 5 to lift upward. After the return lift structure 309 descends, the one-way stopper 4081 acts as an elastic return Flip down to a horizontal state and block the bottom of the return buffer rack 408, thereby supporting the filled culture dish 5 in the return buffer rack 408 and the return storage rack 404 when there is no need to return the material. Therefore, the third push structure 409 does not need to be provided with a clamping structure to clamp the lowermost petri dish 5 in the return storage rack 404 when returning the material. The fourth clamping structure only needs to fill the return storage rack 404 completely. Clamping without clamping the lowermost culture dish 5 in the material storage rack 404 after each completed returning of a filled culture dish 5 . When the material return storage rack 404 rotates to the right above the material return buffer rack 408, the third push structure 409 is used to promote the fourth clamping structure to be in a loose state, so that the material return lifting structure 309 can continuously push the filled culture dish 5 upwards into the material return storage rack 404 until the material return storage rack 404 is full.
以上所述仅为本发明的几个实施例,本领域的技术人员依据申请文件公开的内容可以对本发明实施例进行各种改动或变型而不脱离本发明的精神和范围。The above are only a few embodiments of the present invention, and those skilled in the art can make various changes or modifications to the embodiments of the present invention according to the contents disclosed in the application documents without departing from the spirit and scope of the present invention.

Claims (16)

  1. 一种培养皿灌装设备,其中,包括:A petri dish filling equipment, including:
    设备架;equipment rack;
    灌装机构,安装在所述设备架上,所述灌装机构具有出料管;The filling mechanism is installed on the equipment frame, and the filling mechanism has a discharge pipe;
    储存机构,安装在所述设备架上,所述储存机构上存放有多个待灌装的培养皿;The storage mechanism is installed on the equipment rack, and a plurality of culture dishes to be filled are stored on the storage mechanism;
    移动机构,安装在所述设备架上,所述移动机构能将多个所述培养皿逐个转移至所述出料管的下方,所述出料管可移动至所述培养皿的多个孔位处进行灌装。The moving mechanism is installed on the equipment frame, and the moving mechanism can transfer a plurality of the culture dishes to the bottom of the discharge pipe one by one, and the discharge pipe can be moved to a plurality of holes of the culture dishes for filling.
  2. 根据权利要求1所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 1, wherein,
    所述出料管能沿所述培养皿的长度方向和宽度方向移动设置。The discharge pipe can be moved along the length direction and the width direction of the culture dish.
  3. 根据权利要求2所述的培养皿灌装设备,其中,所述灌装机构包括:The culture dish filling device according to claim 2, wherein the filling mechanism comprises:
    横向运动结构,安装在所述设备架上;a lateral movement structure installed on the equipment frame;
    纵向运动结构,沿所述培养皿的长度方向与所述横向运动结构滑动配合,所述纵向运动结构具有一纵向滑动座,所述纵向滑动座沿所述培养皿的宽度方向滑动设置,所述出料管安装在所述纵向滑动座上。The longitudinal movement structure is slidingly matched with the transverse movement structure along the length direction of the culture dish, the longitudinal movement structure has a longitudinal sliding seat, the longitudinal sliding seat is slidably arranged along the width direction of the culture dish, and the discharge pipe is installed on the longitudinal sliding seat.
  4. 根据权利要求3所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 3, wherein,
    所述横向运动结构包括横向滑轨,所述纵向运动结构沿所述培养皿的长度方向与所述横向滑轨滑动配合,所述横向滑轨的一侧设有废料槽,所述纵向运动结构可带动所述出料管移动至所述废料槽的上方。The transverse movement structure includes a transverse slide rail, and the longitudinal movement structure is slidably matched with the transverse slide rail along the length direction of the culture dish. One side of the transverse slide rail is provided with a waste trough, and the longitudinal movement structure can drive the discharge pipe to move above the waste trough.
  5. 根据权利要求1所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 1, wherein,
    所述移动机构包括第一转盘,所述第一转盘上设有至少一培养皿放置槽,所述第一转盘可带动至少一所述培养皿放置槽移动至所述储存机构的下方。The moving mechanism includes a first turntable, on which at least one culture dish placement slot is provided, and the first turntable can drive at least one culture dish placement slot to move to the bottom of the storage mechanism.
  6. 根据权利要求5所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 5, wherein,
    所述移动机构包括第二转盘,所述第二转盘位于所述第一转盘的上方,且所述第二转盘与所述第一转盘同步转动设置,所述第二转盘上设有至少一培养皿通过孔,所述培养皿上盖合有培养皿盖,待灌装的所述培养皿穿过所述培养皿通过孔并置于所述培养皿放置槽中,所述培养皿盖被所述第二转盘拦截并置于所述第二转盘上,所述出料管从所述第一转盘和所述第二转盘之间伸至待灌装的所述培养皿的上方。The moving mechanism includes a second turntable, the second turntable is located above the first turntable, and the second turntable is rotated synchronously with the first turntable, the second turntable is provided with at least one petri dish through hole, the petri dish is covered with a petri dish cover, the petri dish to be filled passes through the petri dish through hole and placed in the petri dish placement groove, the petri dish cover is intercepted by the second turntable and placed on the second turntable, and the discharge pipe extends from between the first turntable and the second turntable to the culture dish to be filled the top of the dish.
  7. 根据权利要求6所述的培养皿灌装设备,其中,The petri dish filling device according to claim 6, wherein,
    所述第二转盘上设有至少一第一夹紧结构,所述第一夹紧结构能在所述培养皿与所 述培养皿盖分离的状态下将所述培养皿盖夹紧并在所述培养皿与所述培养皿盖重新盖合的状态下将所述培养皿盖松开。The second turntable is provided with at least one first clamping structure, and the first clamping structure can clamp the petri dish cover when the petri dish is separated from the petri dish cap and loosen the petri dish cap when the petri dish and the petri dish cap are re-closed.
  8. 根据权利要求7所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 7, wherein,
    所述第一夹紧结构包括两个夹板,两个所述夹板位于所述培养皿通过孔的两侧,两个所述夹板能朝靠近彼此的方向移动而将所述培养皿盖夹紧,所述设备架上设有推动结构,所述推动结构能推动两个所述夹板朝远离彼此的方向移动而将所述培养皿盖松开。The first clamping structure includes two splints, and the two splints are located on both sides of the passage hole of the culture dish. The two splints can move toward each other to clamp the lid of the petri dish. The equipment frame is provided with a pushing structure, and the pushing structure can push the two splints to move away from each other to loosen the lid of the petri dish.
  9. 根据权利要求5所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 5, wherein,
    所述第一转盘上设有至少三个所述培养皿放置槽,至少三个所述培养皿放置槽沿所述第一转盘的周向间隔排布。The first turntable is provided with at least three petri dish placement slots, and at least three petri dish placement slots are arranged at intervals along the circumference of the first turntable.
  10. 根据权利要求9所述的培养皿灌装设备,其中,Petri dish filling equipment according to claim 9, wherein,
    所述移动机构通过固定安装板安装在所述设备架上,所述固定安装板上设有至少一进料升降结构,所述第一转盘安装在所述固定安装板上,所述第一转盘可带动至少一所述培养皿放置槽移动至至少一所述进料升降结构的上方,所述进料升降结构可带动所述储存机构上待灌装的所述培养皿下降至所述培养皿放置槽内。The moving mechanism is installed on the equipment frame through a fixed mounting plate, the fixed mounting plate is provided with at least one feeding lifting structure, the first turntable is installed on the fixed mounting plate, the first rotating disk can drive at least one of the culture dish placement grooves to move above the at least one feeding lifting structure, and the feeding lifting structure can drive the culture dishes to be filled on the storage mechanism to drop into the culture dish placement grooves.
  11. 根据权利要求10所述的培养皿灌装设备,其中,The Petri dish filling device according to claim 10, wherein,
    所述固定安装板上设有至少一退料升降结构,且至少一所述进料升降结构和至少一所述退料升降结构位于所述灌装机构的两侧,所述第一转盘可带动至少一所述培养皿放置槽移动至至少一所述退料升降结构的上方,至少一所述退料升降结构可带动所述培养皿放置槽内灌装完的所述培养皿上升至所述储存机构上。The fixed mounting plate is provided with at least one material return lifting structure, and at least one feeding lifting structure and at least one material returning lifting structure are located on both sides of the filling mechanism, the first turntable can drive at least one culture dish placement tank to move to the top of at least one material return lifting structure, and at least one material return lifting structure can drive the culture dish filled in the culture dish placement tank to rise to the storage mechanism.
  12. 根据权利要求11所述的培养皿灌装设备,其中,The petri dish filling device according to claim 11, wherein,
    所述储存机构包括储存转盘,所述储存转盘上设有至少一进料储存架,所述进料储存架上沿其高度方向叠放有多个待灌装的所述培养皿,所述储存转盘可带动至少一所述进料储存架转动至所述进料升降结构的上方。The storage mechanism includes a storage turntable, on which at least one feeding storage rack is arranged, and a plurality of culture dishes to be filled are stacked on the feeding storage rack along its height direction, and the storage turntable can drive at least one feeding storage rack to rotate above the feeding lifting structure.
  13. 根据权利要求12所述的培养皿灌装设备,其中,The petri dish filling device according to claim 12, wherein,
    所述储存转盘上设有至少一进料通过孔,所述进料通过孔位于所述进料储存架的下方所述进料储存架的底部设有第二夹紧结构,所述第二夹紧结构能在无需进料的状态下将所述进料储存架内最底层的所述培养皿夹紧并在进料状态下将该培养皿松开,所述设备架上设有第三夹紧结构,所述第三夹紧结构能在进料状态下将紧邻最底层的所述培养皿夹紧。The storage carousel is provided with at least one feeding through hole, and the feeding through hole is located below the feeding storage rack. The bottom of the feeding storage rack is provided with a second clamping structure, and the second clamping structure can clamp the culture dish at the bottom in the feeding storage rack without feeding and release the culture dish in the feeding state.
  14. 根据权利要求12所述的培养皿灌装设备,其中,The petri dish filling device according to claim 12, wherein,
    所述储存转盘上还设有至少一退料储存架,所述储存转盘可带动至少一所述退料储存架转动至所述退料升降结构的上方,所述退料升降结构能将灌装完的所述培养皿上升并放置于所述退料储存架上。The storage carousel is also provided with at least one material return storage rack, and the storage turntable can drive at least one material return storage rack to rotate above the material return lifting structure, and the material return lifting structure can lift the filled culture dish and place it on the material return storage rack.
  15. 根据权利要求14所述的培养皿灌装设备,其中,The petri dish filling device according to claim 14, wherein,
    所述储存转盘上设有至少一退料通过孔,所述退料通过孔位于所述退料储存架的下方,所述退料储存架的底部设有第四夹紧结构,所述第四夹紧结构能将所述退料储存架内最底层的所述培养皿夹紧。The storage carousel is provided with at least one material return passage hole, the material return passage hole is located below the material return storage rack, the bottom of the material return storage rack is provided with a fourth clamping structure, and the fourth clamping structure can clamp the petri dish at the bottom of the material return storage rack.
  16. 根据权利要求15所述的培养皿灌装设备,其中,The petri dish filling device according to claim 15, wherein,
    所述储存转盘上设有至少一退料缓存架,所述退料缓存架位于所述退料储存架的下方,所述退料缓存架的底部设有单向通行挡块,所述单向通行挡块仅能在所述退料升降结构带动灌装完的所述培养皿上升的状态下开启而使灌装完的所述培养皿进入所述退料缓存架内。The storage carousel is provided with at least one material return buffer rack, the material return buffer rack is located below the material return storage rack, and the bottom of the material return buffer rack is provided with a one-way stopper, and the one-way stopper can only be opened when the material return lifting structure drives the filled culture dish to rise, so that the filled culture dish enters the material return buffer rack.
PCT/CN2022/073435 2022-01-24 2022-01-24 Culture dish filling device WO2023137746A1 (en)

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FR2643891A1 (en) * 1989-03-03 1990-09-07 Armor Equipement Scient DEVICE FOR FILLING PETRI BOXES
JP2012213333A (en) * 2011-03-31 2012-11-08 Rabotekku Kk Sample making device
CN104210704A (en) * 2014-08-29 2014-12-17 清华大学 Culture dish taking and storing device
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EP3228694A1 (en) * 2016-04-04 2017-10-11 Clever Culture Systems AG Input/output system for automatically supplying petri dishes to a processing stage and a carrier for manually supplying petri dishes to an input/output system
CN207976489U (en) * 2018-04-10 2018-10-16 深圳市博辰智控有限公司 A kind of microwell plate sample adding system
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FR2643891A1 (en) * 1989-03-03 1990-09-07 Armor Equipement Scient DEVICE FOR FILLING PETRI BOXES
JP2012213333A (en) * 2011-03-31 2012-11-08 Rabotekku Kk Sample making device
CN104210704A (en) * 2014-08-29 2014-12-17 清华大学 Culture dish taking and storing device
EP3228694A1 (en) * 2016-04-04 2017-10-11 Clever Culture Systems AG Input/output system for automatically supplying petri dishes to a processing stage and a carrier for manually supplying petri dishes to an input/output system
CN206096163U (en) * 2016-09-26 2017-04-12 北京慧荣和科技有限公司 Ames test appearance
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