WO2023123292A1 - Procédé de préparation d'un biocapteur à nanopores et procédé de séquençage - Google Patents

Procédé de préparation d'un biocapteur à nanopores et procédé de séquençage Download PDF

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Publication number
WO2023123292A1
WO2023123292A1 PCT/CN2021/143407 CN2021143407W WO2023123292A1 WO 2023123292 A1 WO2023123292 A1 WO 2023123292A1 CN 2021143407 W CN2021143407 W CN 2021143407W WO 2023123292 A1 WO2023123292 A1 WO 2023123292A1
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WIPO (PCT)
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voltage
membrane
nanopore
preparation
protein
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PCT/CN2021/143407
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English (en)
Chinese (zh)
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王佩如
董宇亮
郑荣荣
季州翔
黎宇翔
曾涛
章文蔚
徐讯
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深圳华大生命科学研究院
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Priority to PCT/CN2021/143407 priority Critical patent/WO2023123292A1/fr
Publication of WO2023123292A1 publication Critical patent/WO2023123292A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de préparation d'un biocapteur à nanopores et un procédé de séquençage. Le procédé de préparation comprend : sous l'action d'une première tension, l'insertion d'une protéine de nanocanal dans un matériau de membrane pour constituer un biocapteur à nanopores ayant un canal de nanopore, la première tension étant une tension incrémentielle. Au moyen de la tension incrémentielle, la fusion de la protéine de nanocanal et du matériau de membrane est favorisée, ce qui permet d'améliorer la stabilité du biocapteur à nanopores, et de réduire la probabilité de glissement de la protéine de nanocanal hors du matériau de membrane.
PCT/CN2021/143407 2021-12-30 2021-12-30 Procédé de préparation d'un biocapteur à nanopores et procédé de séquençage WO2023123292A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2021/143407 WO2023123292A1 (fr) 2021-12-30 2021-12-30 Procédé de préparation d'un biocapteur à nanopores et procédé de séquençage

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PCT/CN2021/143407 WO2023123292A1 (fr) 2021-12-30 2021-12-30 Procédé de préparation d'un biocapteur à nanopores et procédé de séquençage

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WO2023123292A1 true WO2023123292A1 (fr) 2023-07-06

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102203618A (zh) * 2008-10-30 2011-09-28 郭培瑄 用于dna测序和其他用途的膜集成病毒dna包装马达蛋白连接器生物传感器
US20120255862A1 (en) * 2011-03-08 2012-10-11 Electronic Biosciences, Llc Methods for voltage-induced protein incorporation into planar lipid bilayers
CN105637082A (zh) * 2013-10-23 2016-06-01 吉尼亚科技公司 用于生物传感器小孔形成的方法
US20200054999A1 (en) * 2017-05-17 2020-02-20 The Royal Institution For The Advancement Of Learning / Mcgill University Method and apparatus for making a nanopore in a membrane using an electric field applied via a conductive tip
CN110954445A (zh) * 2019-10-31 2020-04-03 四川大学华西医院 一种活细胞生物传感器及其制备方法与应用
CN112119033A (zh) * 2018-02-09 2020-12-22 俄亥俄州国家创新基金会 源自噬菌体的纳米孔传感器
CN112345743A (zh) * 2016-11-24 2021-02-09 牛津纳米孔技术公司 用于控制膜通道插入膜中的设备和方法
CN113260449A (zh) * 2018-12-11 2021-08-13 豪夫迈·罗氏有限公司 用于膜中自限性蛋白质孔插入的系统和方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102203618A (zh) * 2008-10-30 2011-09-28 郭培瑄 用于dna测序和其他用途的膜集成病毒dna包装马达蛋白连接器生物传感器
US20120255862A1 (en) * 2011-03-08 2012-10-11 Electronic Biosciences, Llc Methods for voltage-induced protein incorporation into planar lipid bilayers
CN105637082A (zh) * 2013-10-23 2016-06-01 吉尼亚科技公司 用于生物传感器小孔形成的方法
CN112345743A (zh) * 2016-11-24 2021-02-09 牛津纳米孔技术公司 用于控制膜通道插入膜中的设备和方法
US20200054999A1 (en) * 2017-05-17 2020-02-20 The Royal Institution For The Advancement Of Learning / Mcgill University Method and apparatus for making a nanopore in a membrane using an electric field applied via a conductive tip
CN112119033A (zh) * 2018-02-09 2020-12-22 俄亥俄州国家创新基金会 源自噬菌体的纳米孔传感器
CN113260449A (zh) * 2018-12-11 2021-08-13 豪夫迈·罗氏有限公司 用于膜中自限性蛋白质孔插入的系统和方法
CN110954445A (zh) * 2019-10-31 2020-04-03 四川大学华西医院 一种活细胞生物传感器及其制备方法与应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LIU, HANG: "Development and Preliminary Evaluation of Nanopore Single Molecule Sensors", INFORMATION & TECHNOLOGY, CHINA DOCTORAL DISSERTATIONS/MASTER'S THESES FULL-TEXT DATABASE (DOCTORAL), no. 01, 15 January 2020 (2020-01-15), pages 1 - 76, ISSN: 1674-022X *
NOAKES MATTHEW T.; BRINKERHOFF HENRY; LASZLO ANDREW H.; DERRINGTON IAN M.; LANGFORD KYLE W.; MOUNT JONATHAN W.; BOWMAN JASMINE L.;: "Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 37, no. 6, 22 April 2019 (2019-04-22), New York, pages 651 - 656, XP036797521, ISSN: 1087-0156, DOI: 10.1038/s41587-019-0096-0 *

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