WO2023113249A1 - Carbon sheet planar heating element device capable of far-infrared ray emission and temperature control for cell culturing - Google Patents

Carbon sheet planar heating element device capable of far-infrared ray emission and temperature control for cell culturing Download PDF

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Publication number
WO2023113249A1
WO2023113249A1 PCT/KR2022/017706 KR2022017706W WO2023113249A1 WO 2023113249 A1 WO2023113249 A1 WO 2023113249A1 KR 2022017706 W KR2022017706 W KR 2022017706W WO 2023113249 A1 WO2023113249 A1 WO 2023113249A1
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carbon sheet
heating element
planar heating
sheet planar
cell culture
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PCT/KR2022/017706
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French (fr)
Korean (ko)
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황지영
조인호
박상유
오세영
최영민
박진희
박영수
송태진
Original Assignee
재단법인 한국탄소산업진흥원
이화여자대학교 산학협력단
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Publication of WO2023113249A1 publication Critical patent/WO2023113249A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Definitions

  • the present invention relates to a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture.
  • Cell culture is to cultivate cells isolated from tissues of an organism under artificial conditions, and the cells to be cultured are very diverse, such as animal cells, sperm, eggs, anaerobic cells, microorganisms, and the like. Since cells are sensitively affected by the surrounding environment, it is important to maintain a constant temperature during cell culture.
  • a ceramic lamp heating element using nichrome wire and copper wire was used as a heating element applied to maintain the temperature of cell culture.
  • a heating element does not provide constant heat over the entire heating area, and the temperature of the heating element itself is high at 500 ° C or higher and damage to cells and tissues due to heat is a problem when heating for a long time, and power consumption for high temperature heating is high.
  • Korean Patent Registration (10-1155136) proposes a multi-channel photobioreactor for culturing photosynthetic microorganisms to which a planar heater is applied.
  • An object of the present invention is to provide a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture, which can solve the above problems.
  • a carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture to achieve the above object
  • an elevating stage unit movably connected to the guide shaft and moving in a direction approaching and away from the culture vessel;
  • a movement unit coupled to the upper plate, connected to the elevation stage unit, and moving the elevation stage unit;
  • the elevating stage unit It is coupled to the elevating stage unit and characterized in that it includes a heating unit equipped with a planar heating element for heating the cell culture medium.
  • the heating unit includes a carbon sheet planar heating element, and applies heat to the cell culture medium through the carbon sheet planar heating element. Because of this, it is possible to easily test the effect of far-infrared rays on cells through the far-infrared rays emitted from the carbon sheet planar heating element.
  • a heating unit including a planar heating element of a highly conductive carbon sheet is brought close to a cell culture medium placed on a lower plate through an elevating stage unit and a moving unit to heat the cell culture medium.
  • the height of the heating unit can be variously adjusted in the range of 20mm to 140mm with the lifting stage unit and the moving unit, and the temperature of the carbon sheet surface heating element can be variously adjusted in the range of 25°C to 80°C.
  • a plurality of containers can be placed on the lower plate and irradiated with far-infrared rays at the same time. Therefore, using the present invention, under various far-infrared irradiation conditions (irradiation distance, irradiation temperature), it is possible to test the effect of far-infrared rays on cell culture.
  • a first hydrophobic film and a second hydrophobic film are disposed on the upper and lower surfaces of the carbon sheet planar heating element to be heated on top of the cell culture medium. Accordingly, moisture evaporated from the cell culture medium is prevented from permeating into the carbon sheet planar heating element or the heat insulating block. Because of this, even after the irradiation time of the carbon sheet planar heater, far-infrared rays can be irradiated to cells at a constant temperature in the same state as the first time, and the effect of far-infrared rays on cell culture can be tested under consistent conditions.
  • the number of carbon sheet planar heating elements included in the heating unit may be variously included. Due to this, it is possible to precisely control the amount of heat and far-infrared rays emitted from the carbon sheet planar heating element. Therefore, the temperature range can be divided in more detail, and the effect on cells according to the emission amount of far-infrared rays can be tested under more precise conditions.
  • FIG. 1 is a view showing a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to a first embodiment of the present invention.
  • FIG. 2 is a photograph actually taken of a carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to a first embodiment of the present invention.
  • FIG. 3 is an exploded view of the heating unit shown in FIG. 1;
  • FIG. 4 is a side view of the heating unit shown in FIG. 1;
  • FIG. 5 is a view showing a state in which a culture vessel is placed in the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture shown in FIG. 1.
  • FIG. 6 is a side view of the heating unit of the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to a second embodiment of the present invention.
  • FIG. 7 is a graph (A. Heating to 60 ° C by the CF sheet) showing a temperature change according to an image taken with a thermal imaging camera and a heating unit heated to 60 ° C by a carbon sheet planar heating element, and 60 ° C
  • the carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture includes a lower plate 110, an upper plate 120, It consists of a guide shaft 130, a lift stage unit 140, a moving unit 150, a heating unit 160, and a temperature controller 170.
  • a culture vessel (S) containing cell culture medium is placed on the lower plate 110.
  • At least one culture vessel (S) may be placed on the lower plate 110, and the size and height of the culture vessel (S) may be formed in various ways.
  • the lower plate 110 is manufactured in a size capable of placing about four 96-well plates, and 200x200mm far-infrared radiation can be irradiated within an A4 size.
  • a scale indicator is formed on the upper surface of the lower plate 110 to visually check the size of the culture vessel.
  • the scale indicator may be formed as a grid pattern scale. In this embodiment, by attaching an A4 cutting mat to the lower plate 110 as a scale indicator, the culture vessel S can always be maintained in the same position and arrangement. (See Fig. 2)
  • the upper plate 120 is spaced apart from the lower plate 110 in an upper region of the lower plate 110 .
  • the upper plate 120 and the lower plate 110 are formed in the shape of a rectangular plate having the same size.
  • the guide shaft 130 is connected to the upper plate 120 and the lower plate 110 .
  • the upper plate 120 and the lower plate 110 are spaced apart from each other by the guide shaft 130 .
  • the elevating stage unit 140 is movably connected to the guide shaft 130 and moves in a direction approaching and separating from the culture vessel S placed on the lower plate 110 .
  • the lifting stage unit 140 is composed of a lifting plate 141 and a guide bush 142 .
  • the elevating plate 141 is connected to the moving unit 150 .
  • the elevating plate 141 is made of aluminum. However, it is not limited thereto and may be made of various materials such as acrylic.
  • a support groove 141a is formed in the elevating plate 141 to which a screw shaft 152 to be described later of the moving unit 150 is rotatably coupled.
  • the guide bush 142 is coupled to the elevating plate 141 and is connected to the outer circumferential surface of the guide shaft 130 to be slidably movable.
  • a through hole (not shown) through which the guide shaft 130 passes is formed in the guide bush 142 .
  • the moving unit 150 is coupled to the upper plate 120 and is connected to the lifting stage unit 140 to move the lifting stage unit 140 .
  • the moving unit 150 can adjust the height of the heating unit 160 from the lower plate 110 in a range of 20 mm to 140 mm.
  • a ruler for checking the height of the elevating stage unit 140 may be attached between the upper plate 120 and the lower plate 110 . (See Fig. 2)
  • the moving unit 150 is composed of a fixed hub 151, a screw shaft 152, and a rotating member 153.
  • the fixing hub 151 is coupled to the central region of the upper plate 120 .
  • a screw groove (not shown) to which a spiral screw (not shown) formed on the outer circumferential surface of the screw shaft 152 is connected is formed in the fixing hub 151 .
  • the screw shaft 152 is screwed to the fixing hub 151 and rotatably coupled to the elevating plate 141 .
  • the lower end area of the screw shaft 152 is rotatably coupled to the support groove 141a formed in the elevating plate 141 .
  • the rotating member 153 is coupled to the upper end of the screw shaft 152 and rotates the screw shaft 152.
  • a handle for rotation is used as the rotating member 153 in this embodiment.
  • the screw shaft 152 is manually rotated.
  • the screw shaft 152 may be automatically rotated using a rotating motor as the rotating member 153 .
  • the heating unit 160 is coupled to the elevating stage unit 140 .
  • the heating unit 160 includes a carbon sheet planar heating element 161, a heat insulation block 162, a first hydrophobic film 163, a second hydrophobic film 164, a metal electrode ( 166).
  • the carbon sheet planar heater 161 heats the cell culture medium and emits far-infrared rays to the cell culture medium and cells.
  • Far-infrared rays are physically light in the wavelength range of 3 ⁇ m to 1,000 ⁇ m, and are generally known to have thermal action, drying, biological effect, water activation, aging and growth promotion, penetration action, and radiation action. Recently, due to these various actions, far-infrared rays are known to have useful effects on the human body, such as anti-inflammation, osteoporosis, arthritis, and bone regeneration, and many studies are being conducted on the effects of far-infrared rays on the human body.
  • the carbon sheet planar heating element 161 is made of a highly conductive carbon sheet. Due to this, not only can the temperature of the cell culture solution be maintained, but also the effect of the far-infrared source on the cells can be simultaneously tested using the far-infrared rays emitted by the carbon sheet.
  • the carbon sheet planar heating element 161 is made of 200 ⁇ 200 mm.
  • the carbon sheet planar heating element 161 manufactured in this way can generate heat in a temperature range of 25° C. to 80° C.
  • the characteristics of the carbon sheet planar heating element 161 manufactured in this embodiment are as follows.
  • the insulating block 162 is coupled to the lower surface of the elevating plate 141 of the elevating stage unit 140 .
  • the heat insulating block 162 insulates heat generated from the carbon sheet planar heating element 161 .
  • the insulating block 162 is made of a cork material. However, it is not limited thereto, and may be made of materials such as glass, felt, flame retardant plastic, and urethane board.
  • a temperature measuring device for measuring the temperature of the carbon sheet planar heating element 161 may be mounted on the insulating block 162 .
  • a mounting groove for a temperature measuring device to which a temperature measuring device is mounted is formed on the lower surface of the insulating block 162 .
  • a thermocouple may be installed as the temperature measuring instrument.
  • the first hydrophobic film 163 and the second hydrophobic film 164 are attached to the carbon sheet planar heating element 161 .
  • the upper surface of the first hydrophobic film 163 is bonded to the lower surface of the heat insulating block 162 .
  • the lower surface of the first hydrophobic film 163 is disposed above the planar heating element 161 of the carbon sheet and covers the upper surface of the planar heating element 161 of the carbon sheet.
  • the first hydrophobic film 163 is formed smaller than the carbon sheet planar heating element 161, and does not cover the metal electrodes 166 disposed at both ends of the carbon sheet planar heating element 161.
  • the upper surface of the second hydrophobic film 164 is disposed under the carbon sheet planar heating element 161, and covers a portion of the lower surface of the carbon sheet planar heating element 161, that is, the central region.
  • the first hydrophobic film 163 and the second hydrophobic film 164 are made of thermoplastic polyurethane (TPU) material.
  • TPU thermoplastic polyurethane
  • the first hydrophobic film 163 and the second hydrophobic film 164 prevent moisture evaporated from the cell culture medium from forming on the carbon sheet planar heating element 161 or the heat insulating block 162 or permeating into the inside.
  • a metal electrode 166 is attached to the carbon sheet planar heating element 161.
  • the metal electrodes 166 are attached to both ends of the carbon sheet planar heating element 161 .
  • the metal electrode 166 is attached to the upper surface of the carbon sheet planar heating element 161, but unlike this, it may be attached to the lower surface of the carbon sheet planar heating element 161 or attached to both the upper and lower surfaces.
  • the metal electrode 166 may be made of a metal including copper, aluminum, silver, nickel, tin, or the like, or an alloy thereof.
  • the metal electrode 166 may have a ribbon or line shape. Copper is used as the metal electrode 166 in this embodiment. Attach copper tape to both ends of the carbon sheet planar heating element 161 and connect power.
  • the four sides of the heating unit 160 made of the carbon sheet planar heating element 161, the insulation block 162, the first hydrophobic film 163, the second hydrophobic film 164, and the metal electrode 166 are wrapped with an insulating film.
  • an insulating film polyimide (PI), polypropylene (PP), polyethylene terephthalate (PET), ethylene vinyl acetate (EVA), and the like may be used.
  • the temperature controller 170 adjusts the heating temperature of the carbon sheet planar heating element 161 by controlling the power connected to the carbon sheet planar heating element 161 .
  • the temperature of the cell culture medium is about 37°C and should be maintained at this temperature. Therefore, a process of preheating the lower plate 110 is required before placing the culture container S containing the cell culture medium in the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture.
  • power is connected to the metal electrode 166 of the heating unit 160 to heat the carbon sheet planar heating element 161 to about 60°C.
  • the heating temperature may be adjusted to 60 ° C to 80 ° C according to the height of the heating unit 160 and the size of the culture vessel (S) so as to maintain the culture medium at 37 ° C.
  • the lifting plate 141 of the lifting stage unit 140 is lowered to the lower plate 110 by operating the rotating member 153 of the moving unit 150 .
  • the temperature of the carbon sheet planar heating element 161 in a state where the distance between the heating unit 160 and the lower plate 110 is about 40 mm, the temperature of the carbon sheet planar heating element 161 is maintained at about 60 ° C. and after about 1 minute passes, the lower plate 110 ) becomes about 35 °C ⁇ 40 °C (average 37 °C). (See Fig. 7)
  • the lower plate 110 requires a preheating process of about 30 minutes, and after the preheating process is finished, the elevating plate 141 is raised to widen the gap between the heating unit 160 and the lower plate 110, and then the cell culture medium is contained therein.
  • the culture vessel (S) is placed in the central region of the lower plate (110).
  • the elevating plate 141 is lowered until the distance between the heating unit 160 and the lower plate 110 becomes about 40 mm.
  • the cells and cell culture medium are incubated for about 30 minutes to 4 hours.
  • FIGS. 1 and 6 a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to a second embodiment of the present invention will be described in detail. Reference is made primarily to FIGS. 1 and 6 .
  • the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to the second embodiment of the present invention is different from the first embodiment in the heating unit 260.
  • the heating unit 260 according to the second embodiment includes a plurality of thin carbon sheet planar heating elements. If a plurality of thin carbon sheet planar heating elements are used, the amount of heat and far-infrared rays emitted from the carbon sheet planar heating element can be more accurately and easily controlled than when using one thick carbon sheet planar heating element. Therefore, a more diverse temperature range can be configured, and the effect on cells according to the emission amount of far-infrared rays can be tested under various conditions.
  • the heating unit 260 includes two carbon sheet planar heating elements, and hydrophobic films are attached to upper and lower surfaces of each carbon sheet planar heating element.
  • the number of planar heating elements of the carbon sheet may be formed to be greater than this, and the hydrophobic film may be correspondingly increased.
  • the heating unit 260 is coupled to the elevating stage unit 140 .
  • the heating unit 260 includes a first carbon sheet planar heating element 261a, a second carbon sheet planar heating element 261b, a heat insulating block 262, a first hydrophobic film 263, and a second carbon sheet planar heating element 261b. It is composed of a hydrophobic film 264, a third hydrophobic film 265, and a metal electrode 266. Details identical to those of the heat generating unit 160 according to the first embodiment will be omitted, and will be described focusing on distinct features.
  • the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b are made of highly conductive carbon sheets and have the same size.
  • the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b may be manufactured to have a size of 200 ⁇ 200 mm.
  • the insulating block 262 is the same as the insulating block 162 of the heating unit 160 of the first embodiment.
  • the first hydrophobic film 263 and the second hydrophobic film 264 are attached to the upper and lower surfaces of the first carbon sheet planar heating element 261a, respectively, and the second hydrophobic film 264 and the third hydrophobic film 265 are The second carbon sheet is attached to the upper and lower surfaces of the planar heating element 261b, respectively.
  • the upper surface of the first hydrophobic film 263 is bonded to the lower surface of the heat insulating block 262 .
  • the lower surface of the first hydrophobic film 263 is disposed above the first carbon sheet planar heating element 261a and covers the upper surface of the first carbon sheet planar heating element 261a.
  • the second hydrophobic film 264 has an upper surface disposed below the first carbon sheet planar heating element 261a, and covers the lower surface of the first carbon sheet planar heating element 261a.
  • the lower surface of the second hydrophobic film 264 is disposed above the second carbon sheet planar heating element 261b and covers the upper surface of the second carbon sheet planar heating element 261b.
  • the upper surface of the third hydrophobic film 265 is disposed below the second carbon sheet planar heating element 261b, and covers the lower surface of the second carbon sheet planar heating element 261b.
  • the first hydrophobic film 263 and the second hydrophobic film 264 are formed to have a shorter length than the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, so that the first carbon sheet planar heating element 261a
  • the metal electrodes 266 attached to both ends of the second carbon sheet planar heating element 261b are not covered by the first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265.
  • the first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265 are made of thermoplastic polyurethane (TPU) material.
  • TPU thermoplastic polyurethane
  • first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265 moisture evaporated from the cell culture medium is transferred to the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b. Alternatively, it prevents condensation on the insulating block 262 or permeation into the inside.
  • a metal electrode 266 is used to connect the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b. ) are attached to each.
  • the metal electrode 266 is attached to both ends of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b.
  • the metal electrode 266 is attached to the upper surfaces of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, respectively. It may be attached to each lower surface of the carbon sheet planar heating element 261b or attached to both the upper and lower surfaces.
  • Copper is used as the metal electrode 266 in this embodiment. Copper tape is attached to both ends of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, and power is connected to each metal electrode 266.

Abstract

In the present invention, a heat generating unit including a highly conductive carbon sheet planar heating element is brought close to a cell culture medium placed on a lower plate through an elevating stage unit and a moving unit to heat the cell culture medium. Accordingly, far-infrared rays can be constantly emitted to cells while stable heat generation is performed at a temperature suitable for cell culturing by using low power. In addition, the height of the heat generating unit can be variously adjusted in the range of 20mm to 140mm by using the elevating stage unit and the moving unit, and the temperature of the carbon sheet planar heat generating element can be variously adjusted in the range of 25˚C to 80˚C, thereby performing simultaneous heating multiple culture vessels of various sizes and heights which are placed on the lower plate. Therefore, effects of far-infrared rays on cell culturing can be tested under various far-infrared irradiation conditions (irradiation distance, irradiation temperature) by using the present invention.

Description

세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치Carbon sheet surface heating device capable of emitting far-infrared rays and controlling temperature for cell culture
본 발명은 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치에 관한 것이다.The present invention relates to a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture.
세포배양은 생물체의 조직으로부터 분리된 세포를 인공적인 조건 하에서 배양하는 것으로서, 배양되는 세포는 동물세포, 정자, 난자, 혐기성 세포, 미생물 등으로 매우 다양하다. 세포는 주변의 환경에 민감하게 영향을 받기 때문에 세포가 배양되는 동안 온도를 일정하게 유지해주는 것이 중요하다. Cell culture is to cultivate cells isolated from tissues of an organism under artificial conditions, and the cells to be cultured are very diverse, such as animal cells, sperm, eggs, anaerobic cells, microorganisms, and the like. Since cells are sensitively affected by the surrounding environment, it is important to maintain a constant temperature during cell culture.
종래의 경우, 세포배양의 온도를 유지하기 위해 적용되는 발열체로 니크롬선 및 구리선을 이용한 세라믹램프 발열체가 사용되었다. 그러나 이러한 발열체는 발열면적 전체에 걸쳐 일정한 열을 제공하지 못하고, 발열체 자체의 온도가 500℃ 이상의 고온으로 장시간 발열시에 열에 의한 세포 및 조직의 손상이 문제 되며, 고온 발열을 위한 전력 소비가 크다는 문제가 있다.In the conventional case, a ceramic lamp heating element using nichrome wire and copper wire was used as a heating element applied to maintain the temperature of cell culture. However, such a heating element does not provide constant heat over the entire heating area, and the temperature of the heating element itself is high at 500 ° C or higher and damage to cells and tissues due to heat is a problem when heating for a long time, and power consumption for high temperature heating is high. there is
이러한 문제를 해결하기 위하여, 한국등록특허(10-1155136)에서는, 면상발열체가 적용된 광합성 미생물 배양을 위한 다채널 광생물 반응기를 제시하고 있다.In order to solve this problem, Korean Patent Registration (10-1155136) proposes a multi-channel photobioreactor for culturing photosynthetic microorganisms to which a planar heater is applied.
그러나, 이러한 다채널 광생물 반응기로는, 다양한 원적외선 조사 환경에서, 원적외선이 세포배양에 미치는 효과를 테스트할 수 없다.However, with this multi-channel photobioreactor, the effect of far-infrared rays on cell culture cannot be tested under various far-infrared irradiation environments.
본 발명의 목적은, 상술한 문제점을 해결할 수 있는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치를 제공하는 데 있다.An object of the present invention is to provide a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture, which can solve the above problems.
상기 목적을 달성하기 위한 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치는,A carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture to achieve the above object,
세포 배양액이 담긴 배양용기가 놓이는 하부 플레이트;A lower plate on which a culture vessel containing cell culture medium is placed;
상기 하부 플레이트의 상부 영역에 상기 하부 플레이트에 대해 이격되어 배치되는 상부 플레이트;an upper plate disposed spaced apart from the lower plate in an upper region of the lower plate;
상기 상부 플레이트와 상기 하부 플레이트에 연결되는 가이드 샤프트;guide shafts connected to the upper plate and the lower plate;
상기 가이드 샤프트에 이동 가능하게 연결되며, 상기 배양용기에 대해 접근 및 이격되는 방향으로 이동되는 승강 스테이지유닛;an elevating stage unit movably connected to the guide shaft and moving in a direction approaching and away from the culture vessel;
상기 상부 플레이트에 결합되고, 상기 승강 스테이지유닛에 연결되며, 상기 승강 스테이지유닛을 이동시키는 이동유닛; 및a movement unit coupled to the upper plate, connected to the elevation stage unit, and moving the elevation stage unit; and
상기 승강 스테이지유닛에 결합되며, 상기 세포 배양액을 가열하는 카본시트 면상발열체가 장착된 발열유닛을 포함하는 것을 특징으로 한다.It is coupled to the elevating stage unit and characterized in that it includes a heating unit equipped with a planar heating element for heating the cell culture medium.
본 발명은 발열유닛이 카본시트 면상발열체를 포함하여, 카본시트 면상발열체를 통해 세포 배양액에 열을 가한다. 이로 인해, 카본시트 면상발열체에서 방출되는 원적외선을 통해, 원적외선이 세포에 미치는 영향을 쉽게 테스트해 볼 수 있다.In the present invention, the heating unit includes a carbon sheet planar heating element, and applies heat to the cell culture medium through the carbon sheet planar heating element. Because of this, it is possible to easily test the effect of far-infrared rays on cells through the far-infrared rays emitted from the carbon sheet planar heating element.
본 발명은 고전도성의 카본시트 면상발열체를 포함하는 발열유닛을 승강 스테이지유닛과 이동유닛을 통해 하부 플레이트에 놓인 세포 배양액에 가까이 근접시켜 세포 배양액을 가열한다. 이로 인해, 저전력으로 세포배양에 적정한 온도에서 안정하게 발열하면서 세포에 일정하게 원적외선을 조사할 수 있다. 또한, 승강 스테이지유닛과 이동유닛으로 발열유닛의 높이를 20mm~140mm 범위에서 다양하게 조절하고, 카본시트 면상발열체의 온도를 25℃~80℃ 범위에서 다양하게 조절할 수 있어, 크기와 높이가 다양한 배양용기 다수 개를 하부 플레이트에 두고 동시에 원적외선 조사가 가능하다. 따라서, 본 발명을 사용하면, 다양한 원적외선 조사 조건(조사 거리, 조사 온도)에서, 원적외선이 세포배양에 미치는 효과를 테스트할 수 있다.According to the present invention, a heating unit including a planar heating element of a highly conductive carbon sheet is brought close to a cell culture medium placed on a lower plate through an elevating stage unit and a moving unit to heat the cell culture medium. As a result, it is possible to constantly irradiate far-infrared rays to cells while stably generating heat at a temperature suitable for cell culture with low power. In addition, the height of the heating unit can be variously adjusted in the range of 20mm to 140mm with the lifting stage unit and the moving unit, and the temperature of the carbon sheet surface heating element can be variously adjusted in the range of 25℃ to 80℃. A plurality of containers can be placed on the lower plate and irradiated with far-infrared rays at the same time. Therefore, using the present invention, under various far-infrared irradiation conditions (irradiation distance, irradiation temperature), it is possible to test the effect of far-infrared rays on cell culture.
본 발명은 세포 배양액의 상부에 가열하는 카본시트 면상발열체의 상면과 하면에 제1소수성필름과 제2소수성필름이 배치된다. 이로 인해, 세포 배양액에서 증발한 수분이 카본시트 면상발열체 또는 단열블록에 스며드는 것이 방지된다. 이로 인해, 카본시트 면상발열체가 조사 시간이 지나도 처음과 같은 상태로, 원적외선을 일정한 온도로 세포에 조사할 수 있어, 원적외선이 세포배양에 미치는 효과를 일관된 조건하에서 테스트할 수 있다.In the present invention, a first hydrophobic film and a second hydrophobic film are disposed on the upper and lower surfaces of the carbon sheet planar heating element to be heated on top of the cell culture medium. Accordingly, moisture evaporated from the cell culture medium is prevented from permeating into the carbon sheet planar heating element or the heat insulating block. Because of this, even after the irradiation time of the carbon sheet planar heater, far-infrared rays can be irradiated to cells at a constant temperature in the same state as the first time, and the effect of far-infrared rays on cell culture can be tested under consistent conditions.
본 발명은 발열유닛이 포함하는 카본시트 면상발열체의 개수를 다양하게 포함할 수 있다. 이로 인해, 카본시트 면상발열체에서 방출되는 열과 원적외선의 방출량을 정밀하게 조절할 수 있다. 따라서 온도 범위를 더 세밀하게 나눌 수 있고, 원적외선의 방출량에 따른 세포에 미치는 영향을 더 정밀한 조건하에서 테스트해 볼 수 있다.In the present invention, the number of carbon sheet planar heating elements included in the heating unit may be variously included. Due to this, it is possible to precisely control the amount of heat and far-infrared rays emitted from the carbon sheet planar heating element. Therefore, the temperature range can be divided in more detail, and the effect on cells according to the emission amount of far-infrared rays can be tested under more precise conditions.
도 1은 본 발명의 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치를 나타낸 도면이다.1 is a view showing a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to a first embodiment of the present invention.
도 2는 본 발명의 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치를 실제로 찍은 사진이다.2 is a photograph actually taken of a carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to a first embodiment of the present invention.
도 3은 도 1에 도시된 발열유닛을 분해한 도면이다.3 is an exploded view of the heating unit shown in FIG. 1;
도 4는 도 1에 도시된 발열유닛의 측면도이다.4 is a side view of the heating unit shown in FIG. 1;
도 5는 도 1에 도시된 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치에 배양용기가 놓인 상태를 나타낸 도면이다.5 is a view showing a state in which a culture vessel is placed in the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture shown in FIG. 1.
도 6은 본 발명의 제2실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치의 발열유닛의 측면도이다.6 is a side view of the heating unit of the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to a second embodiment of the present invention.
도 7은 카본시트 면상발열체에 의해 60℃로 가열된 발열유닛을 열화상카메라로 촬영한 영상 및 가열시간에 따른 온도변화를 나타낸 그래프(A. Heating to 60℃ by the CF sheet)와, 60℃에서 가열된 하부 플레이트를 열화상카메라로 촬영한 영상과 가열시간에 따른 온도변화를 나타낸 그래프(B. Cutting Mat at 60℃)를 나타낸다.7 is a graph (A. Heating to 60 ° C by the CF sheet) showing a temperature change according to an image taken with a thermal imaging camera and a heating unit heated to 60 ° C by a carbon sheet planar heating element, and 60 ° C The graph (B. Cutting Mat at 60 ℃) showing the temperature change according to the heating time and the image taken with the thermal imaging camera of the lower plate heated in .
이하, 본 발명의 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치를 자세히 설명한다.Hereinafter, a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to a first embodiment of the present invention will be described in detail.
도 1 및 도 2에 도시된 바와 같이, 본 발명의 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치는, 하부 플레이트(110), 상부 플레이트(120), 가이드 샤프트(130), 승강 스테이지유닛(140), 이동유닛(150), 발열유닛(160), 온도조절기(170)로 구성된다. As shown in FIGS. 1 and 2, the carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to the first embodiment of the present invention includes a lower plate 110, an upper plate 120, It consists of a guide shaft 130, a lift stage unit 140, a moving unit 150, a heating unit 160, and a temperature controller 170.
[하부 플레이트(110), 상부 플레이트(120), 가이드 샤프트(130)][Lower plate 110, upper plate 120, guide shaft 130]
하부 플레이트(110)에는 세포 배양액이 담긴 배양용기(S)가 놓인다. 배양용기(S)는 하나 이상이 하부 플레이트(110)에 놓을 수 있고, 배양용기(S)의 크기와 높이는 다양하게 형성될 수 있다. 일예로, 하부 플레이트(110)는96-웰 플레이트(96-well plate)를 4개 정도 올려놓을 수 있는 크기로 제작되며, A4 사이즈 내에서 200x200mm 원적외선 조사가 가능하다. On the lower plate 110, a culture vessel (S) containing cell culture medium is placed. At least one culture vessel (S) may be placed on the lower plate 110, and the size and height of the culture vessel (S) may be formed in various ways. For example, the lower plate 110 is manufactured in a size capable of placing about four 96-well plates, and 200x200mm far-infrared radiation can be irradiated within an A4 size.
하부 플레이트(110)의 상면에는 배양용기의 크기를 시각적으로 확인할 수 있는 눈금표시기가 형성된다. 눈금표시기는 격자무늬 눈금으로 형성될 수 있다. 본 실시예에서는 하부 플레이트(110)에 눈금표시기로 A4 커팅 매트(cutting mat)를 부착하여 배양용기(S)를 항상 동일한 위치 및 배열을 유지할 수 있다. (도 2 참조)A scale indicator is formed on the upper surface of the lower plate 110 to visually check the size of the culture vessel. The scale indicator may be formed as a grid pattern scale. In this embodiment, by attaching an A4 cutting mat to the lower plate 110 as a scale indicator, the culture vessel S can always be maintained in the same position and arrangement. (See Fig. 2)
상부 플레이트(120)는 하부 플레이트(110)의 상부 영역에 하부 플레이트(110)에 대해 이격되어 배치된다.The upper plate 120 is spaced apart from the lower plate 110 in an upper region of the lower plate 110 .
본 실시예에서 상부 플레이트(120)와 하부 플레이트(110)는 동일한 크기의 사각판 형상으로 형성된다.In this embodiment, the upper plate 120 and the lower plate 110 are formed in the shape of a rectangular plate having the same size.
가이드 샤프트(130)는 상부 플레이트(120)와 하부 플레이트(110)에 연결된다. 상부 플레이트(120)와 하부 플레이트(110)는 가이드 샤프트(130)에 의해 서로 이격된다.The guide shaft 130 is connected to the upper plate 120 and the lower plate 110 . The upper plate 120 and the lower plate 110 are spaced apart from each other by the guide shaft 130 .
[승강 스테이지유닛(140)][Elevating stage unit 140]
승강 스테이지유닛(140)은 가이드 샤프트(130)에 이동 가능하게 연결되며, 하부 플레이트(110)에 놓인 배양용기(S)에 대해 접근 및 이격되는 방향으로 이동된다.The elevating stage unit 140 is movably connected to the guide shaft 130 and moves in a direction approaching and separating from the culture vessel S placed on the lower plate 110 .
승강 스테이지유닛(140)은 승강 플레이트(141), 가이드 부시(142)로 구성된다. The lifting stage unit 140 is composed of a lifting plate 141 and a guide bush 142 .
승강 플레이트(141)는 이동유닛(150)에 연결된다. 본 실시예에서 승강 플레이트(141)는 알루미늄 재질로 제작된다. 그러나 이에 한정되지 않으며 아크릴 등 다양한 재질로 제작될 수 있다. 승강 플레이트(141)에는 이동유닛(150)의 후술할 스크류 샤프트(152)가 회전 가능하게 결합되는 지지홈(141a)이 형성된다.The elevating plate 141 is connected to the moving unit 150 . In this embodiment, the elevating plate 141 is made of aluminum. However, it is not limited thereto and may be made of various materials such as acrylic. A support groove 141a is formed in the elevating plate 141 to which a screw shaft 152 to be described later of the moving unit 150 is rotatably coupled.
가이드 부시(142)는 승강 플레이트(141)에 결합되며, 가이드 샤프트(130)의 외주면에 슬라이딩 이동 가능하게 연결된다. 가이드 부시(142)에는 가이드 샤프트(130)가 관통하는 관통홀(미도시)이 형성된다. The guide bush 142 is coupled to the elevating plate 141 and is connected to the outer circumferential surface of the guide shaft 130 to be slidably movable. A through hole (not shown) through which the guide shaft 130 passes is formed in the guide bush 142 .
[이동유닛(150)][Movement unit 150]
이동유닛(150)은 상부 플레이트(120)에 결합되고, 승강 스테이지유닛(140)에 연결되며, 승강 스테이지유닛(140)을 이동시킨다. 이동유닛(150)은 발열유닛(160)을 하부 플레이트(110)로부터 20mm~140mm 범위에서 높이 조절이 가능하다. 승강 스테이지유닛(140)의 높이를 확인하기 위한 자가 상부 플레이트(120)와 하부 플레이트(110) 사이에 부착될 수 있다. (도 2 참조)The moving unit 150 is coupled to the upper plate 120 and is connected to the lifting stage unit 140 to move the lifting stage unit 140 . The moving unit 150 can adjust the height of the heating unit 160 from the lower plate 110 in a range of 20 mm to 140 mm. A ruler for checking the height of the elevating stage unit 140 may be attached between the upper plate 120 and the lower plate 110 . (See Fig. 2)
이동유닛(150)은 고정 허브(151), 스크류 샤프트(152), 회전부재(153)로 구성된다. The moving unit 150 is composed of a fixed hub 151, a screw shaft 152, and a rotating member 153.
고정 허브(151)는 상부 플레이트(120)의 중앙영역에 결합된다. 고정 허브(151)에는 스크류 샤프트(152)의 외주면에 형성된 나선형의 스크류(미도시)가 연결되는 나사홈(미도시) 형성된다. The fixing hub 151 is coupled to the central region of the upper plate 120 . A screw groove (not shown) to which a spiral screw (not shown) formed on the outer circumferential surface of the screw shaft 152 is connected is formed in the fixing hub 151 .
스크류 샤프트(152)는 고정 허브(151)에 나사결합되며, 승강 플레이트(141)에 회전 가능하게 결합된다. 스크류 샤프트(152)의 하단부 영역은, 승강 플레이트(141)에 형성된 지지홈(141a)에 회전 가능하게 결합된다. The screw shaft 152 is screwed to the fixing hub 151 and rotatably coupled to the elevating plate 141 . The lower end area of the screw shaft 152 is rotatably coupled to the support groove 141a formed in the elevating plate 141 .
회전부재(153)는 스크류 샤프트(152)의 상단부에 결합되며 스크류 샤프트(152)를 회전시킨다. 본 실시예에서 회전부재(153)로 회전용 손잡이가 사용된다. 회전부재(153)가 회전용 손잡이인 경우 스크류 샤프트(152)를 수동으로 회전시킨다. 이와 달리 회전부재(153)로 회전용 모터를 사용하여 스크류 샤프트(152)를 자동으로 회전시킬 수도 있다.The rotating member 153 is coupled to the upper end of the screw shaft 152 and rotates the screw shaft 152. A handle for rotation is used as the rotating member 153 in this embodiment. When the rotating member 153 is a handle for rotation, the screw shaft 152 is manually rotated. Alternatively, the screw shaft 152 may be automatically rotated using a rotating motor as the rotating member 153 .
[발열유닛(160)][heating unit 160]
발열유닛(160)은 승강 스테이지유닛(140)에 결합된다. The heating unit 160 is coupled to the elevating stage unit 140 .
도 3 및 도 4에 도시된 바와 같이, 발열유닛(160)은 카본시트 면상발열체(161), 단열블록(162), 제1소수성필름(163), 제2소수성필름(164), 금속전극(166)으로 구성된다. 3 and 4, the heating unit 160 includes a carbon sheet planar heating element 161, a heat insulation block 162, a first hydrophobic film 163, a second hydrophobic film 164, a metal electrode ( 166).
<카본시트 면상발열체(161)><Carbon Sheet Planar Heating Element (161)>
카본시트 면상발열체(161)는 세포 배양액을 가열하며, 세포 배양액 및 세포로 원적외선을 방출한다. The carbon sheet planar heater 161 heats the cell culture medium and emits far-infrared rays to the cell culture medium and cells.
원적외선은 물리학적으로 3㎛∼1,000㎛ 파장대의 빛으로, 일반적으로 온열작용, 건조, 생체효과, 물의 활성화, 숙성 및 생육촉진, 침투작용, 복사작용 등이 있는 것으로 알려져 있다. 최근 이런 다양한 작용으로 인해, 원적외선이 항염증, 골다공증, 관절염, 뼈재생 등 인체에 유용한 효과가 있다고 알려지면서, 원적외선이 인체에 미치는 영향에 대한 많은 연구가 이루어지고 있다.Far-infrared rays are physically light in the wavelength range of 3 μm to 1,000 μm, and are generally known to have thermal action, drying, biological effect, water activation, aging and growth promotion, penetration action, and radiation action. Recently, due to these various actions, far-infrared rays are known to have useful effects on the human body, such as anti-inflammation, osteoporosis, arthritis, and bone regeneration, and many studies are being conducted on the effects of far-infrared rays on the human body.
카본시트 면상발열체(161)는 고전도성 카본시트로 만들어진다. 이로 인해, 세포 배양액의 온도를 유지할 수 있을 뿐만 아니라 카본시트가 방출하는 원적외선을 이용하여 원적외원이 세포에 미치는 영향에 대해서도 동시에 테스트해 볼 수 있다. The carbon sheet planar heating element 161 is made of a highly conductive carbon sheet. Due to this, not only can the temperature of the cell culture solution be maintained, but also the effect of the far-infrared source on the cells can be simultaneously tested using the far-infrared rays emitted by the carbon sheet.
본 실시예에서 카본시트 면상발열체(161)는 200×200mm로 제작된다. 이렇게 제작된 카본시트 면상발열체(161)는 25℃~80℃의 온도 범위에서 발열이 가능하다.In this embodiment, the carbon sheet planar heating element 161 is made of 200 × 200 mm. The carbon sheet planar heating element 161 manufactured in this way can generate heat in a temperature range of 25° C. to 80° C.
본 실시예에서 제작된 카본시트 면상발열체(161)의 특성은 다음과 같다.The characteristics of the carbon sheet planar heating element 161 manufactured in this embodiment are as follows.
카본시트 면상발열체의 전기적 특성Electrical Characteristics of Carbon Sheet Planar Heating Element
Thickness (mm)Thickness (mm) Surface resistance (ohm/sq)Surface resistance (ohm/sq) Volume resistivity Volume resistivity
(ohm·cm)(ohm cm) 		Electrical conductivity (S/cm)Electrical conductivity (S/cm)
실시예Example 0.462 ± 0.0180.462 ± 0.018 4.378 ± 0.3884.378 ± 0.388 0.202 ± 0.0190.202 ± 0.019 4.996 ± 0.5434.996 ± 0.543
카본시트 면상발열체의 원적외선 방출 특성Far-infrared ray emission characteristics of carbon sheet planar heating element
Emissivity Emissivity
(5~20 μm, (5~20 μm,
37℃)37℃) 		Radiation energy Radiation energy
(W/㎡·μm, (W/㎡ μm,
37℃)37℃) 		Emissivity Emissivity
(5~20 μm, (5~20 μm,
60℃)60℃) 		Radiation energy Radiation energy
(W/㎡·μm, (W/㎡ μm,
60℃)60℃)
실시예Example 0.9180.918 3.54 x 102 3.54 x 10 2 0.9140.914 4.84 x 102 4.84 x 10 2
<단열블록(162)><Insulation block (162)>
단열블록(162)은 승강 스테이지유닛(140)의 승강 플레이트(141)의 하면에 결합된다. 단열블록(162)은 카본시트 면상발열체(161)에서 발생한 열을 단열한다. 본 실시예에서 단열블록(162)은 코르크 재질로 제작된다. 그러나 이에 한정되지 않으며, 유리면, 펠트, 난연플라스틱, 우레탄보드 등의 재질로 제작될 수 있다.The insulating block 162 is coupled to the lower surface of the elevating plate 141 of the elevating stage unit 140 . The heat insulating block 162 insulates heat generated from the carbon sheet planar heating element 161 . In this embodiment, the insulating block 162 is made of a cork material. However, it is not limited thereto, and may be made of materials such as glass, felt, flame retardant plastic, and urethane board.
단열블록(162)에는 카본시트 면상발열체(161)의 온도를 측정하는 온도 측정기가 장착될 수 있다. 이 경우, 단열블록(162)의 하면에는 온도 측정기가 장착되는 온도 측정기용 장착홈이 형성된다. 온도 측정기로는 써모커플(Thermocouple)이 장착될 수 있다.A temperature measuring device for measuring the temperature of the carbon sheet planar heating element 161 may be mounted on the insulating block 162 . In this case, a mounting groove for a temperature measuring device to which a temperature measuring device is mounted is formed on the lower surface of the insulating block 162 . A thermocouple may be installed as the temperature measuring instrument.
<제1소수성필름(163), 제2소수성필름(164)><First hydrophobic film 163, second hydrophobic film 164>
제1소수성필름(163)과 제2소수성필름(164)은 카본시트 면상발열체(161)에 부착된다. The first hydrophobic film 163 and the second hydrophobic film 164 are attached to the carbon sheet planar heating element 161 .
제1소수성필름(163)은 상면이 단열블록(162)의 하면에 결합된다. 제1소수성필름(163)의 하면은 카본시트 면상발열체(161)의 상부에 배치되며, 카본시트 면상발열체(161)의 상면을 커버한다. 제1소수성필름(163)은 카본시트 면상발열체(161)보다 작게 형성되어, 카본시트 면상발열체(161)의 양단부에 배치된 금속전극(166)은 커버하지 않는다. The upper surface of the first hydrophobic film 163 is bonded to the lower surface of the heat insulating block 162 . The lower surface of the first hydrophobic film 163 is disposed above the planar heating element 161 of the carbon sheet and covers the upper surface of the planar heating element 161 of the carbon sheet. The first hydrophobic film 163 is formed smaller than the carbon sheet planar heating element 161, and does not cover the metal electrodes 166 disposed at both ends of the carbon sheet planar heating element 161.
제2소수성필름(164)은 상면이 카본시트 면상발열체(161)의 하부에 배치되며, 카본시트 면상발열체(161)의 하면의 일부분 즉, 중앙영역을 커버한다. The upper surface of the second hydrophobic film 164 is disposed under the carbon sheet planar heating element 161, and covers a portion of the lower surface of the carbon sheet planar heating element 161, that is, the central region.
본 실시예에서 제1소수성필름(163)과 제2소수성필름(164)은 열가소성 폴리우레탄(thermoplastic polyurethane, TPU) 재질로 제작된다. 그러나 이에 한정되지 않으며, 폴리에틸렌 테레프탈레이트(polyethylene terephthalate, PET), 에틸렌초산비닐(Ethylene Vinyl Acetate, EVA) 등의 필름으로 제작될 수 있다.In this embodiment, the first hydrophobic film 163 and the second hydrophobic film 164 are made of thermoplastic polyurethane (TPU) material. However, it is not limited thereto, and may be made of a film such as polyethylene terephthalate (PET) or ethylene vinyl acetate (EVA).
제1소수성필름(163)과 제2소수성필름(164)은 세포 배양액에서 증발된 수분이 카본시트 면상발열체(161)나 단열블록(162)에 맺히거나 내부로 스며드는 것을 방지한다. The first hydrophobic film 163 and the second hydrophobic film 164 prevent moisture evaporated from the cell culture medium from forming on the carbon sheet planar heating element 161 or the heat insulating block 162 or permeating into the inside.
<금속전극(166)><Metal electrode 166>
카본시트 면상발열체(161)에 전원을 연결하기 위해, 금속전극(166)이 카본시트 면상발열체(161)에 부착된다. 금속전극(166)은 카본시트 면상발열체(161)의 양단부에 부착된다. 본 실시예에서 금속전극(166)은 카본시트 면상발열체(161)의 상면에 부착되었으나, 이와 달리 카본시트 면상발열체(161)의 하면에 부착되거나 상면과 하면 모두에 부착될 수 있다.In order to connect power to the carbon sheet planar heating element 161, a metal electrode 166 is attached to the carbon sheet planar heating element 161. The metal electrodes 166 are attached to both ends of the carbon sheet planar heating element 161 . In this embodiment, the metal electrode 166 is attached to the upper surface of the carbon sheet planar heating element 161, but unlike this, it may be attached to the lower surface of the carbon sheet planar heating element 161 or attached to both the upper and lower surfaces.
금속전극(166)은 구리, 알루미늄, 은, 니켈, 주석 등을 포함하는 금속 또는 이들의 합금으로 이루어지질 수 있다. 금속전극(166)은 리본 또는 선 형태일 수 있다. 본 실시예에서 금속전극(166)으로 구리가 사용된다. 카본시트 면상발열체(161)의 양단부에 구리테이프를 붙이고 전원을 연결한다.The metal electrode 166 may be made of a metal including copper, aluminum, silver, nickel, tin, or the like, or an alloy thereof. The metal electrode 166 may have a ribbon or line shape. Copper is used as the metal electrode 166 in this embodiment. Attach copper tape to both ends of the carbon sheet planar heating element 161 and connect power.
카본시트 면상발열체(161), 단열블록(162), 제1소수성필름(163), 제2소수성필름(164), 금속전극(166)으로 만들어진 발열유닛(160)의 네 측면은 절연필름으로 감싸진다. 절연필름으로는 폴리이미드(polyimide, PI), 폴리프로필렌(polypropylene, PP), 폴리에틸렌 테레프탈레이트(polyethylene terephthalate, PET), 에틸렌초산비닐(Ethylene Vinyl Acetate, EVA) 등이 사용될 수 있다. The four sides of the heating unit 160 made of the carbon sheet planar heating element 161, the insulation block 162, the first hydrophobic film 163, the second hydrophobic film 164, and the metal electrode 166 are wrapped with an insulating film. lose As the insulating film, polyimide (PI), polypropylene (PP), polyethylene terephthalate (PET), ethylene vinyl acetate (EVA), and the like may be used.
[온도조절기(170)][Thermostat (170)]
온도조절기(170)는 카본시트 면상발열체(161)에 연결되는 전원을 조절하여 카본시트 면상발열체(161)의 발열온도를 조절한다.The temperature controller 170 adjusts the heating temperature of the carbon sheet planar heating element 161 by controlling the power connected to the carbon sheet planar heating element 161 .
이하, 본 발명의 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치의 작동을 자세히 설명한다.Hereinafter, the operation of the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to the first embodiment of the present invention will be described in detail.
세포 배양액의 온도는 약 37℃이며 이 온도를 유지해야 한다. 따라서 세포 배양액이 담긴 배양용기(S)를 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치에 배치하기 전에, 하부 플레이트(110)를 예열하는 과정이 필요하다.The temperature of the cell culture medium is about 37°C and should be maintained at this temperature. Therefore, a process of preheating the lower plate 110 is required before placing the culture container S containing the cell culture medium in the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture.
본 실시예에서는 발열유닛(160)의 금속전극(166)에 전원을 연결하여 카본시트 면상발열체(161)의 온도를 약 60℃로 가열한다. 가열온도는 배양액을 37℃로 유지할 수 있도록, 발열유닛(160)의 높이와 배양용기(S)의 크기에 따라 60℃~80℃로 조절될 수 있다. 이동유닛(150)의 회전부재(153)를 작동시켜 승강 스테이지유닛(140)의 승강 플레이트(141)를 하부 플레이트(110)로 하강시킨다.In this embodiment, power is connected to the metal electrode 166 of the heating unit 160 to heat the carbon sheet planar heating element 161 to about 60°C. The heating temperature may be adjusted to 60 ° C to 80 ° C according to the height of the heating unit 160 and the size of the culture vessel (S) so as to maintain the culture medium at 37 ° C. The lifting plate 141 of the lifting stage unit 140 is lowered to the lower plate 110 by operating the rotating member 153 of the moving unit 150 .
본 실시예에서는 발열유닛(160)과 하부 플레이트(110) 사이의 간격이 약 40mm인 상태에서, 카본시트 면상발열체(161)의 온도를 약 60℃로 유지하고 약 1분이 지나면, 하부 플레이트(110)의 온도가 약 35℃~40℃(평균 37℃)가 된다. (도 7 참조)In this embodiment, in a state where the distance between the heating unit 160 and the lower plate 110 is about 40 mm, the temperature of the carbon sheet planar heating element 161 is maintained at about 60 ° C. and after about 1 minute passes, the lower plate 110 ) becomes about 35 ℃ ~ 40 ℃ (average 37 ℃). (See Fig. 7)
하부 플레이트(110)은 30분 정도의 예열과정이 필요하며, 예열과정이 끝나면 승강 플레이트(141)를 상승시켜 발열유닛(160)과 하부 플레이트(110) 사이의 간격을 벌린 후, 세포 배양액이 담긴 배양용기(S)를 하부 플레이트(110)의 중앙 영역에 배치시킨다. The lower plate 110 requires a preheating process of about 30 minutes, and after the preheating process is finished, the elevating plate 141 is raised to widen the gap between the heating unit 160 and the lower plate 110, and then the cell culture medium is contained therein. The culture vessel (S) is placed in the central region of the lower plate (110).
도 5에 도시된 바와 같이, 발열유닛(160)과 하부 플레이트(110) 사이의 간격이 약 40mm이 될 때까지 승강 플레이트(141)를 하강시킨다. 세포 및 세포 배양액을 약 30분~4시간 동안 배양시킨다.As shown in FIG. 5 , the elevating plate 141 is lowered until the distance between the heating unit 160 and the lower plate 110 becomes about 40 mm. The cells and cell culture medium are incubated for about 30 minutes to 4 hours.
이하, 본 발명의 제2실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치를 자세히 설명한다. 도 1 및 도 6을 기본적으로 참조한다. Hereinafter, a carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture according to a second embodiment of the present invention will be described in detail. Reference is made primarily to FIGS. 1 and 6 .
본 발명의 제2실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치는, 발열유닛(260)에서 제1실시예와 차이가 있다. 제2실시예에 따른 발열유닛(260)은 얇은 카본시트 면상발열체 복수 개를 구비한다. 얇은 카본시트 면상발열체를 복수 개 사용하면, 두꺼운 카본시트 면상발열체를 한 장 사용할 때 보다, 카본시트 면상발열체에서 방출되는 열과 원적외선의 방출량을 보다 정밀하고 쉽게 조절할 수 있다. 따라서 온도 범위를 더 다양하게 구성할 수 있고, 원적외선의 방출량에 따른 세포에 미치는 영향을 다양한 조건하에서 테스트해 볼 수 있다.The carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to the second embodiment of the present invention is different from the first embodiment in the heating unit 260. The heating unit 260 according to the second embodiment includes a plurality of thin carbon sheet planar heating elements. If a plurality of thin carbon sheet planar heating elements are used, the amount of heat and far-infrared rays emitted from the carbon sheet planar heating element can be more accurately and easily controlled than when using one thick carbon sheet planar heating element. Therefore, a more diverse temperature range can be configured, and the effect on cells according to the emission amount of far-infrared rays can be tested under various conditions.
제2실시예에서는 발열유닛(260)이 두 개의 카본시트 면상발열체를 포함하고, 각 카본시트 면상발열체의 상면과 하면에 소수성필름이 부착된다. 그러나 이에 한정되지 않고, 카본시트 면상발열체의 개수는 이보다 더 많게 형성될 수 있고, 이에 상응하여 소수성필름도 증가될 수 있다.In the second embodiment, the heating unit 260 includes two carbon sheet planar heating elements, and hydrophobic films are attached to upper and lower surfaces of each carbon sheet planar heating element. However, it is not limited thereto, and the number of planar heating elements of the carbon sheet may be formed to be greater than this, and the hydrophobic film may be correspondingly increased.
나머지 구성은 제1실시예와 동일하므로, 동일한 구성에 대한 자세한 설명은 생략한다. 동일한 구성에 대해서는 동일한 부호를 사용한다.Since the rest of the configuration is the same as that of the first embodiment, a detailed description of the same configuration will be omitted. Identical symbols are used for identical configurations.
[발열유닛(260)][heating unit 260]
발열유닛(260)은 승강 스테이지유닛(140)에 결합된다. The heating unit 260 is coupled to the elevating stage unit 140 .
도 6에 도시된 바와 같이, 발열유닛(260)은 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b), 단열블록(262), 제1소수성필름(263), 제2소수성필름(264), 제3소수성필름(265), 금속전극(266)으로 구성된다. 제1실시예에 따른 발열유닛(160)과 동일한 내용은 자세한 설명을 생략하고, 구별되는 특징을 중심으로 설명하기로 한다. As shown in FIG. 6, the heating unit 260 includes a first carbon sheet planar heating element 261a, a second carbon sheet planar heating element 261b, a heat insulating block 262, a first hydrophobic film 263, and a second carbon sheet planar heating element 261b. It is composed of a hydrophobic film 264, a third hydrophobic film 265, and a metal electrode 266. Details identical to those of the heat generating unit 160 according to the first embodiment will be omitted, and will be described focusing on distinct features.
<제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)><First carbon sheet planar heating element 261a and second carbon sheet planar heating element 261b>
제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)는 고전도성 카본시트로 만들어지며, 동일한 크기로 만들어진다. 본 실시예에서 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)는 200×200mm로 제작될 수 있다. The first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b are made of highly conductive carbon sheets and have the same size. In this embodiment, the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b may be manufactured to have a size of 200×200 mm.
<단열블록(262)><Insulation block (262)>
단열블록(262)은 제1실시예의 발열유닛(160)의 단열블록(162)과 동일하다.The insulating block 262 is the same as the insulating block 162 of the heating unit 160 of the first embodiment.
<제1소수성필름(263), 제2소수성필름(264), 제3소수성필름(265)><First hydrophobic film 263, second hydrophobic film 264, third hydrophobic film 265>
제1소수성필름(263)과 제2소수성필름(264)은 제1카본시트 면상발열체(261a)의 상면과 하면에 각각 부착되고, 제2소수성필름(264)과 제3소수성필름(265)은 제2카본시트 면상발열체(261b)의 상면과 하면에 각각 부착된다.The first hydrophobic film 263 and the second hydrophobic film 264 are attached to the upper and lower surfaces of the first carbon sheet planar heating element 261a, respectively, and the second hydrophobic film 264 and the third hydrophobic film 265 are The second carbon sheet is attached to the upper and lower surfaces of the planar heating element 261b, respectively.
제1소수성필름(263)은 상면이 단열블록(262)의 하면에 결합된다. 제1소수성필름(263)의 하면은 제1카본시트 면상발열체(261a)의 상부에 배치되어, 제1카본시트 면상발열체(261a)의 상면을 커버한다.The upper surface of the first hydrophobic film 263 is bonded to the lower surface of the heat insulating block 262 . The lower surface of the first hydrophobic film 263 is disposed above the first carbon sheet planar heating element 261a and covers the upper surface of the first carbon sheet planar heating element 261a.
제2소수성필름(264)은 상면이 제1카본시트 면상발열체(261a)의 하부에 배치되어, 제1카본시트 면상발열체(261a)의 하면을 커버한다. 제2소수성필름(264)의 하면은 제2카본시트 면상발열체(261b)의 상부에 배치되어, 제2카본시트 면상발열체(261b)의 상면을 커버한다.The second hydrophobic film 264 has an upper surface disposed below the first carbon sheet planar heating element 261a, and covers the lower surface of the first carbon sheet planar heating element 261a. The lower surface of the second hydrophobic film 264 is disposed above the second carbon sheet planar heating element 261b and covers the upper surface of the second carbon sheet planar heating element 261b.
제3소수성필름(265)은 상면이 제2카본시트 면상발열체(261b)의 하부에 배치되어, 제2카본시트 면상발열체(261b)의 하면을 커버한다.The upper surface of the third hydrophobic film 265 is disposed below the second carbon sheet planar heating element 261b, and covers the lower surface of the second carbon sheet planar heating element 261b.
제1소수성필름(263)과 제2소수성필름(264)은 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)보다는 길이가 작게 형성되어 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)의 양단부에 부착된 금속전극(266)은 제1소수성필름(263)과 제2소수성필름(264)과 제3소수성필름(265)에 의해 커버되지 않는다.The first hydrophobic film 263 and the second hydrophobic film 264 are formed to have a shorter length than the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, so that the first carbon sheet planar heating element 261a The metal electrodes 266 attached to both ends of the second carbon sheet planar heating element 261b are not covered by the first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265.
본 실시예에서 제1소수성필름(263)과 제2소수성필름(264)과 제3소수성필름(265)은 열가소성 폴리우레탄(thermoplastic polyurethane, TPU) 재질로 제작된다. 그러나 이에 한정되지 않으며, 폴리에틸렌 테레프탈레이트(polyethylene terephthalate, PET), 에틸렌초산비닐(Ethylene Vinyl Acetate, EVA) 등의 필름으로 제작될 수 있다.In this embodiment, the first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265 are made of thermoplastic polyurethane (TPU) material. However, it is not limited thereto, and may be made of a film such as polyethylene terephthalate (PET) or ethylene vinyl acetate (EVA).
제1소수성필름(263)과 제2소수성필름(264)과 제3소수성필름(265)은 세포 배양액에서 증발된 수분이 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b) 또는 단열블록(262)에 맺히거나 내부로 스며드는 것을 방지한다. In the first hydrophobic film 263, the second hydrophobic film 264, and the third hydrophobic film 265, moisture evaporated from the cell culture medium is transferred to the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b. Alternatively, it prevents condensation on the insulating block 262 or permeation into the inside.
<금속전극(266)><Metal electrode 266>
제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)에 전원을 연결하기 위해, 금속전극(266)이 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)에 각각 부착된다. 금속전극(266)은 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)의 양단부에 부착된다. 본 실시예에서 금속전극(266)은 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)의 상면에 각각 부착되었으나, 이와 달리 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)의 하면에 각각 부착되거나 상면과 하면 모두에 부착될 수도 있다.In order to connect power to the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, a metal electrode 266 is used to connect the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b. ) are attached to each. The metal electrode 266 is attached to both ends of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b. In this embodiment, the metal electrode 266 is attached to the upper surfaces of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, respectively. It may be attached to each lower surface of the carbon sheet planar heating element 261b or attached to both the upper and lower surfaces.
본 실시예에서 금속전극(266)으로 구리가 사용된다. 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)의 양단부에 구리테이프를 붙이고, 각 금속전극(266)에 전원을 연결한다. Copper is used as the metal electrode 266 in this embodiment. Copper tape is attached to both ends of the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b, and power is connected to each metal electrode 266.
이하, 본 발명의 제2실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치의 작동을 자세히 설명한다. 도 1 및 도 6을 기본적으로 참조한다. Hereinafter, the operation of the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to a second embodiment of the present invention will be described in detail. Reference is made primarily to FIGS. 1 and 6 .
기본적으로는 제1실시예에 따른 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치의 작동과 동일하므로, 제1실시예와 구별되는 작동을 중심으로 설명하기로 한다.Since it is basically the same as the operation of the carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture according to the first embodiment, the operation differentiated from the first embodiment will be mainly described.
발열유닛(260)의 금속전극(266)에 전원을 연결할 때, 제1실시예와 같은 온도 범위와 원적외선의 방출량으로 시험하고자 할 때에는 제2카본시트 면상발열체(261b)에만 전원을 공급한다.When power is connected to the metal electrode 266 of the heat generating unit 260, power is supplied only to the second carbon sheet planar heating element 261b when testing is performed in the same temperature range and far-infrared emission amount as in the first embodiment.
이와 달리, 제1실시예의 온도 범위와 원적외선의 방출량보다 더 큰 범위의 열을 방출하고자 할 때에는 제1카본시트 면상발열체(261a)와 제2카본시트 면상발열체(261b)에 전원을 공급한다.Unlike this, when it is desired to emit heat in a range larger than the temperature range and far-infrared emission amount of the first embodiment, power is supplied to the first carbon sheet planar heating element 261a and the second carbon sheet planar heating element 261b.

Claims (5)

  1. 세포 배양액이 담긴 배양용기가 놓이는 하부 플레이트; A lower plate on which a culture vessel containing cell culture medium is placed;
    상기 하부 플레이트의 상부 영역에 상기 하부 플레이트에 대해 이격되어 배치되는 상부 플레이트;an upper plate disposed spaced apart from the lower plate in an upper region of the lower plate;
    상기 상부 플레이트와 상기 하부 플레이트에 연결되는 가이드 샤프트;guide shafts connected to the upper plate and the lower plate;
    상기 가이드 샤프트에 이동 가능하게 연결되며, 상기 배양용기에 대해 접근 및 이격되는 방향으로 이동되는 승강 스테이지유닛;an elevating stage unit movably connected to the guide shaft and moving in a direction approaching and away from the culture container;
    상기 상부 플레이트에 결합되고, 상기 승강 스테이지유닛에 연결되며, 상기 승강 스테이지유닛을 이동시키는 이동유닛; 및a movement unit coupled to the upper plate, connected to the elevation stage unit, and moving the elevation stage unit; and
    상기 승강 스테이지유닛에 결합되며, 상기 세포 배양액을 가열하는 카본시트 면상발열체가 장착된 발열유닛을 포함하는 것을 특징으로 하는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치.A carbon sheet planar heating element device capable of emitting far-infrared rays and controlling temperature for cell culture, characterized in that it includes a heating unit coupled to the lifting stage unit and equipped with a carbon sheet planar heating element for heating the cell culture medium.
  2. 제1항에 있어서,According to claim 1,
    상기 발열유닛은,The heating unit is
    상기 카본시트 면상발열체;The carbon sheet planar heating element;
    상기 승강 스테이지유닛의 하면에 결합되는 단열블록;an insulating block coupled to a lower surface of the elevating stage unit;
    상기 단열블록의 하면에 결합되며, 상기 카본시트 면상발열체의 상부에 배치되어 상기 카본시트 면상발열체의 상면을 커버하는 제1소수성필름; a first hydrophobic film coupled to a lower surface of the heat insulating block and disposed on an upper surface of the carbon sheet planar heating element to cover an upper surface of the carbon sheet planar heating element;
    상기 카본시트 면상발열체의 하부에 배치되어 상기 카본시트 면상발열체의 하면을 커버하는 제2소수성필름; 및a second hydrophobic film disposed under the carbon sheet planar heating element to cover the lower surface of the carbon sheet planar heating element; and
    상기 카본시트 면상발열체에 부착되는 금속전극을 포함하는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치.A carbon sheet planar heating element device capable of emitting far-infrared rays and controlling temperature for cell culture including a metal electrode attached to the carbon sheet planar heating element.
  3. 제2항에 있어서,According to claim 2,
    상기 단열블록에는,In the insulation block,
    상기 카본시트 면상발열체의 온도를 측정하는 온도 측정기가 장착되는 것을 특징으로 하는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치.A carbon sheet planar heating element device capable of emitting far-infrared rays and controlling temperature for cell culture, characterized in that a temperature measuring device for measuring the temperature of the carbon sheet planar heating element is mounted.
  4. 제1항에 있어서,According to claim 1,
    상기 승강 스테이지유닛은, The lifting stage unit,
    상기 이동유닛에 연결되는 승강 플레이트; 및an elevation plate connected to the moving unit; and
    상기 승강 플레이트에 결합되며, 상기 가이드 샤프트의 외주면에 슬라이딩 이동 가능하게 연결되는 가이드 부시를 포함하는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치.A carbon sheet planar heating device capable of emitting far-infrared rays and controlling temperature for cell culture, including a guide bush coupled to the elevating plate and movably connected to an outer circumferential surface of the guide shaft.
  5. 제4항에 있어서, According to claim 4,
    상기 이동유닛은,The mobile unit,
    상기 상부 플레이트에 결합되는 고정 허브;a fixed hub coupled to the upper plate;
    상기 고정 허브에 나사결합되며, 상기 승강 플레이트에 회전 가능하게 결합되는 스크류 샤프트; 및a screw shaft screwed to the fixing hub and rotatably coupled to the elevating plate; and
    상기 스크류 샤프트에 결합되며 상기 스크류 샤프트를 회전시키는 회전부재를 포함하는 세포배양을 위한 원적외선 방출 및 온도조절이 가능한 카본시트 면상발열체 장치.A carbon sheet planar heating element capable of emitting far-infrared rays and controlling temperature for cell culture comprising a rotation member coupled to the screw shaft and rotating the screw shaft.
PCT/KR2022/017706 2021-12-16 2022-11-11 Carbon sheet planar heating element device capable of far-infrared ray emission and temperature control for cell culturing WO2023113249A1 (en)

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